RchiOBHm_Chr4g0406551

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001684
Physical Location & Seq
Forward (+)
28369897 .. 28370508
612 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37789

Sequence Viewer

Length: 540 bp
ATGTCCATGGACGCAGATCCCAATGTTACTACTCTTGTGACTGTTTTGTCTTCGTGTTCTAGTTTAAGAGCTTTCAAGTTAGGTAAAGCTGTTCATGGCCATTGTTTGAGGAACGTACACAAGTGTAATATGATTTTGGACAATGCAATGTTGGATTTCTATCTGAGATGTGGTTCTTTGGTGGCTGCAAGATACTTGTTTGTCATTATGCCCAAGAGAGATTTTGTTTCTTGGAGTAGCATGGTGGGTGGTTATGCACATAGAGGTTTATGTGAGGAGGCAATGAGACTTTTCCAACAACTGGTGCTGGGCTGGGAAGTTGAGCCGAATGAGGCTACCATTGTCAATGTATTGTCAGCCTGTTCTTCTGTTTGTTCCTTGAGTTCAAGCCAGCAGGTGCATTTCTATGTCAGTACTCAGCCAGATCTCATGGTGAATAGCAATGTGGGAAATGCCTGGATCAACATGTATGTTAGGAGTGGACATTTGGGTATGGCTATTTCGGTTTTTAAGGCCTTGGAGTGCAAAGATTTCATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

179

Amino Acids

19.85

Weight (kDa)

7.6

Isoelectric Point (pI)

43.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 76 - 101 6.8e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 385
AasI GACNNNNNNGTC 1 cut(s) 46
Acc36I ACCTGC 1 cut(s) 385
AccB7I CCANNNNNTGG 1 cut(s) 301
AclWI GGATC 2 cut(s) 11, 467
AcoI YGGCCR 1 cut(s) 97
AfaI GTAC 2 cut(s) 117, 415
AfiI CCNNNNNNNGG 1 cut(s) 301
AflIII ACRYGT 1 cut(s) 465
AgsI TTSAA 2 cut(s) 76, 387
AjnI CCWGG 1 cut(s) 455
AluBI AGCT 2 cut(s) 71, 89
AluI AGCT 2 cut(s) 71, 89
Alw26I GTCTC 1 cut(s) 280
AlwI GGATC 2 cut(s) 11, 467
AoxI GGCC 2 cut(s) 97, 513
ApeKI GCWGC 1 cut(s) 185
AsuHPI GGTGA 1 cut(s) 445
BalI TGGCCA 1 cut(s) 99
BbsI GAAGAC 1 cut(s) 42
BbvI GCAGC 1 cut(s) 172
BciT130I CCWGG 1 cut(s) 457
BcoDI GTCTC 1 cut(s) 280
BfaI CTAG 1 cut(s) 60
BfuAI ACCTGC 1 cut(s) 385
BglII AGATCT 1 cut(s) 424
BisI GCNGC 1 cut(s) 186
BlsI GCNGC 1 cut(s) 187
BmcAI AGTACT 1 cut(s) 415
Bme1390I CCNGG 1 cut(s) 457
BmrFI CCNGG 1 cut(s) 457
BpiI GAAGAC 1 cut(s) 42
BpuEI CTTGAG 1 cut(s) 400
BsaBI GATNNNNATC 1 cut(s) 159
BsaJI CCNNGG 2 cut(s) 6, 516
Bsc4I CCNNNNNNNGG 1 cut(s) 301
Bse1I ACTGG 1 cut(s) 306
Bse3DI GCAATG 3 cut(s) 153, 288, 448
Bse8I GATNNNNATC 1 cut(s) 159
BseBI CCWGG 1 cut(s) 457
BseDI CCNNGG 2 cut(s) 6, 516
BseJI GATNNNNATC 1 cut(s) 159
BseLI CCNNNNNNNGG 1 cut(s) 301
BseMI GCAATG 3 cut(s) 153, 288, 448
BseMII CTCAG 2 cut(s) 155, 431
BseNI ACTGG 1 cut(s) 306
BseRI GAGGAG 1 cut(s) 290
BseXI GCAGC 1 cut(s) 172
BseYI CCCAGC 2 cut(s) 307, 312
BshFI GGCC 2 cut(s) 99, 515
BslI CCNNNNNNNGG 1 cut(s) 301
BsmAI GTCTC 1 cut(s) 280
BsnI GGCC 2 cut(s) 99, 515
Bsp143I GATC 3 cut(s) 16, 424, 459
Bsp19I CCATGG 1 cut(s) 6
BspANI GGCC 2 cut(s) 99, 515
BspCNI CTCAG 2 cut(s) 156, 430
BspMI ACCTGC 1 cut(s) 385
BspPI GGATC 2 cut(s) 11, 467
BsrDI GCAATG 3 cut(s) 153, 288, 448
BsrI ACTGG 1 cut(s) 306
BssECI CCNNGG 2 cut(s) 6, 516
BssMI GATC 3 cut(s) 16, 424, 459
BssT1I CCWWGG 2 cut(s) 6, 516
Bst2UI CCWGG 1 cut(s) 457
Bst4CI ACNGT 1 cut(s) 43
BstC8I GCNNGC 1 cut(s) 392
BstDEI CTNAG 2 cut(s) 164, 417
BstDSI CCRYGG 1 cut(s) 6
BstKTI GATC 3 cut(s) 19, 427, 462
BstMAI GTCTC 1 cut(s) 280
BstMBI GATC 3 cut(s) 16, 424, 459
BstNI CCWGG 1 cut(s) 457
BstNSI RCATGY 1 cut(s) 469
BstSCI CCNGG 1 cut(s) 455
BstV1I GCAGC 1 cut(s) 172
BstV2I GAAGAC 1 cut(s) 42
BstX2I RGATCY 2 cut(s) 16, 424
BstYI RGATCY 2 cut(s) 16, 424
BsuRI GGCC 2 cut(s) 99, 515
BtgI CCRYGG 1 cut(s) 6
BveI ACCTGC 1 cut(s) 385
Cac8I GCNNGC 1 cut(s) 392
CseI GACGC 1 cut(s) 20
Csp6I GTAC 2 cut(s) 116, 414
CviAII CATG 5 cut(s) 7, 95, 241, 430, 466
CviQI GTAC 2 cut(s) 116, 414
DdeI CTNAG 2 cut(s) 164, 417
DpnI GATC 3 cut(s) 18, 426, 461
DpnII GATC 3 cut(s) 16, 424, 459
DrdI GACNNNNNNGTC 1 cut(s) 46
DseDI GACNNNNNNGTC 1 cut(s) 46
EaeI YGGCCR 1 cut(s) 97
Eco130I CCWWGG 2 cut(s) 6, 516
Eco147I AGGCCT 1 cut(s) 515
EcoRII CCWGG 1 cut(s) 455
EcoT14I CCWWGG 2 cut(s) 6, 516
ErhI CCWWGG 2 cut(s) 6, 516
FaeI CATG 5 cut(s) 10, 98, 244, 433, 469
FatI CATG 5 cut(s) 6, 94, 240, 429, 465
Fnu4HI GCNGC 1 cut(s) 186
Fsp4HI GCNGC 1 cut(s) 186
FspBI CTAG 1 cut(s) 60
GluI GCNGC 1 cut(s) 186
GsaI CCCAGC 2 cut(s) 311, 316
HaeIII GGCC 2 cut(s) 99, 515
HgaI GACGC 1 cut(s) 20
Hin1II CATG 5 cut(s) 10, 98, 244, 433, 469
HphI GGTGA 1 cut(s) 445
Hpy166II GTNNAC 2 cut(s) 118, 482
Hpy188I TCNGA 1 cut(s) 165
Hpy8I GTNNAC 2 cut(s) 118, 482
HpyCH4III ACNGT 1 cut(s) 43
HpyCH4IV ACGT 1 cut(s) 114
HpyCH4V TGCA 5 cut(s) 146, 188, 257, 400, 525
HpyF3I CTNAG 2 cut(s) 164, 417
HpySE526I ACGT 1 cut(s) 114
Hsp92II CATG 5 cut(s) 10, 98, 244, 433, 469
Kzo9I GATC 3 cut(s) 16, 424, 459
LpnPI CCDG 9 cut(s) 287, 293, 298, 373, 380, 404, 435, 442, 469
Lsp1109I GCAGC 1 cut(s) 172
MaeI CTAG 1 cut(s) 60
MaeII ACGT 1 cut(s) 114
MaeIII GTNAC 2 cut(s) 25, 37
MalI GATC 3 cut(s) 18, 426, 461
MboI GATC 3 cut(s) 16, 424, 459
MboII GAAGA 2 cut(s) 42, 357
MflI RGATCY 2 cut(s) 16, 424
MlsI TGGCCA 1 cut(s) 99
MluNI TGGCCA 1 cut(s) 99
MmeI TCCRAC 2 cut(s) 132, 319
MnlI CCTC 5 cut(s) 102, 257, 268, 271, 325
Mox20I TGGCCA 1 cut(s) 99
MscI TGGCCA 1 cut(s) 99
MseI TTAA 3 cut(s) 65, 510, 538
MslI CAYNNNNRTG 1 cut(s) 405
Msp20I TGGCCA 1 cut(s) 99
MspR9I CCNGG 1 cut(s) 457
MvaI CCWGG 1 cut(s) 457
NcoI CCATGG 1 cut(s) 6
NdeII GATC 3 cut(s) 16, 424, 459
NlaIII CATG 5 cut(s) 10, 98, 244, 433, 469
NmuCI GTSAC 1 cut(s) 37
NspI RCATGY 1 cut(s) 469
PaqCI CACCTGC 1 cut(s) 385
PceI AGGCCT 1 cut(s) 515
PciI ACATGT 1 cut(s) 465
PflMI CCANNNNNTGG 1 cut(s) 301
PkrI GCNGC 1 cut(s) 187
PscI ACATGT 1 cut(s) 465
Psp6I CCWGG 1 cut(s) 455
PspFI CCCAGC 2 cut(s) 307, 312
PspGI CCWGG 1 cut(s) 455
PsuI RGATCY 2 cut(s) 16, 424
RsaI GTAC 2 cut(s) 117, 415
RsaNI GTAC 2 cut(s) 116, 414
RseI CAYNNNNRTG 1 cut(s) 405
SaqAI TTAA 3 cut(s) 65, 510, 538
SatI GCNGC 1 cut(s) 186
Sau3AI GATC 3 cut(s) 16, 424, 459
ScaI AGTACT 1 cut(s) 415
ScrFI CCNGG 1 cut(s) 457
SetI ASST 6 cut(s) 73, 85, 91, 117, 268, 399
SmiMI CAYNNNNRTG 1 cut(s) 405
SmlI CTYRAG 1 cut(s) 379
SmoI CTYRAG 1 cut(s) 379
SseBI AGGCCT 1 cut(s) 515
SspMI CTAG 1 cut(s) 60
StuI AGGCCT 1 cut(s) 515
StyD4I CCNGG 1 cut(s) 455
StyI CCWWGG 2 cut(s) 6, 516
TaaI ACNGT 1 cut(s) 43
TaiI ACGT 1 cut(s) 117
TatI WGTACW 1 cut(s) 413
Tru1I TTAA 3 cut(s) 65, 510, 538
Tru9I TTAA 3 cut(s) 65, 510, 538
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 1 cut(s) 185
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 2 cut(s) 83, 523
Van91I CCANNNNNTGG 1 cut(s) 301
XceI RCATGY 1 cut(s) 469
XspI CTAG 1 cut(s) 60
ZrmI AGTACT 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.