Rmu_ssc0000186.1_g000036

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000186.1
OG0001684
Physical Location & Seq
Forward (+)
198607 .. 203677
5071 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000186.1_g000036.1.cds

Sequence Viewer

Length: 1017 bp
atggacgcagaacccaatgttactactcttgtgactgttttgtcttcgtgttctagtttaagagctttcaagttaggtaaagctgttcatggccattgtttgaggaacgtacacgactataatatgattttggacaatgcaatgttggatttctatctgagatgtggttctttggtggctgcaagatacttgtttgtcattatgcccaagagagattttgtttcttggagtagcatggtgggtggttatgcacatagaggtttatgtgaggaggcaatgagacttttccaacaactggtgctgggctgggaagttgagccgaatgaggctaccattgtcaatgtattgtcagcatgttcttctgtttgttccttgagttcaagccagcaggtgcatttctatatcagtactcagccagatctcatggtgaatagcaatatgggaaatgccttgattaacatgtacgttaggagtggacatttgggtatggctatttcggtttttaaggccttggagtgcaaagatatcattttatggagtaccataataagcgggtcgaggggaagagtcaaaaggcagaaatttgcaaatgctgaggagaattttgagagaatcaagtacaaggtcaaaaggagggagaagtcatcctccaccttgctgtgctccggtgaccagtggccagtcgcggtctccggtgacaggagtccggcgtccagtcaccggaatcccgaatccgactaccggactggcgcccagctcctcttctctcttctttccggttgcagattcggaacatggcgacacaccctacatctgttcgtcgggtttcagcccacggtgggaagtgtgcacaacgctattgtcaatgataggaagctcgtccacgtggttaagaggacgatatacccgaagaaaaccttgtcgttgggaggagatcagagtcgcagacgtcgatttatatggaggttgacgtcacagacgtcgaatgcgcggcggagatcggaggaagacgactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

338

Amino Acids

38.12

Weight (kDa)

9.66

Isoelectric Point (pI)

53.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 379
AasI GACNNNNNNGTC 1 cut(s) 40
AatII GACGTC 3 cut(s) 952, 974, 983
Acc36I ACCTGC 1 cut(s) 379
AccB1I GGYRCC 1 cut(s) 749
AccB7I CCANNNNNTGG 1 cut(s) 295
AccII CGCG 2 cut(s) 686, 991
AciI CCGC 4 cut(s) 552, 686, 991, 994
AcoI YGGCCR 2 cut(s) 91, 677
AcsI RAATTY 2 cut(s) 581, 601
AcvI CACGTG 1 cut(s) 886
AcyI GRCGYC 5 cut(s) 710, 750, 949, 971, 980
AfaI GTAC 5 cut(s) 111, 409, 464, 541, 620
AfiI CCNNNNNNNGG 5 cut(s) 295, 699, 720, 741, 839
AflIII ACRYGT 1 cut(s) 459
AgsI TTSAA 2 cut(s) 70, 381
AluBI AGCT 4 cut(s) 65, 83, 757, 877
AluI AGCT 4 cut(s) 65, 83, 757, 877
Alw21I GWGCWC 2 cut(s) 665, 852
Alw26I GTCTC 2 cut(s) 274, 694
Alw44I GTGCAC 1 cut(s) 848
AoxI GGCC 3 cut(s) 91, 507, 677
ApaLI GTGCAC 1 cut(s) 848
ApeKI GCWGC 1 cut(s) 179
ApoI RAATTY 2 cut(s) 581, 601
AspLEI GCGC 2 cut(s) 752, 991
AsuHPI GGTGA 4 cut(s) 439, 680, 707, 710
BaeGI GKGCMC 1 cut(s) 852
BalI TGGCCA 2 cut(s) 93, 679
BanI GGYRCC 1 cut(s) 749
BbrPI CACGTG 1 cut(s) 886
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 2 cut(s) 665, 852
BbvCI CCTCAGC 1 cut(s) 594
BbvI GCAGC 1 cut(s) 166
BcoDI GTCTC 2 cut(s) 274, 694
BfaI CTAG 1 cut(s) 54
BfoI RGCGCY 1 cut(s) 753
BfuAI ACCTGC 1 cut(s) 379
BglII AGATCT 1 cut(s) 418
BisI GCNGC 2 cut(s) 180, 992
BlsI GCNGC 2 cut(s) 181, 993
BmcAI AGTACT 1 cut(s) 409
BmiI GGNNCC 1 cut(s) 751
BoxI GACNNNNGTC 1 cut(s) 702
BpiI GAAGAC 1 cut(s) 36
Bpu10I CCTNAGC 1 cut(s) 594
BpuEI CTTGAG 1 cut(s) 394
BsaAI YACGTR 1 cut(s) 886
BsaBI GATNNNNATC 1 cut(s) 153
BsaHI GRCGYC 5 cut(s) 710, 750, 949, 971, 980
BsaI GGTCTC 1 cut(s) 694
BsaJI CCNNGG 2 cut(s) 510, 834
BsaWI WCCGGW 5 cut(s) 665, 692, 720, 741, 776
Bsc4I CCNNNNNNNGG 5 cut(s) 295, 699, 720, 741, 839
Bse1I ACTGG 5 cut(s) 300, 673, 680, 714, 751
Bse3DI GCAATG 2 cut(s) 147, 282
Bse8I GATNNNNATC 1 cut(s) 153
BseDI CCNNGG 2 cut(s) 510, 834
BseGI GGATG 1 cut(s) 644
BseJI GATNNNNATC 1 cut(s) 153
BseLI CCNNNNNNNGG 5 cut(s) 295, 699, 720, 741, 839
BseMI GCAATG 2 cut(s) 147, 282
BseMII CTCAG 3 cut(s) 149, 425, 585
BseNI ACTGG 5 cut(s) 300, 673, 680, 714, 751
BseRI GAGGAG 4 cut(s) 284, 611, 749, 945
BseSI GKGCMC 1 cut(s) 852
BseXI GCAGC 1 cut(s) 166
BseYI CCCAGC 3 cut(s) 301, 306, 753
Bsh1236I CGCG 2 cut(s) 686, 991
BshFI GGCC 3 cut(s) 93, 509, 679
BshNI GGYRCC 1 cut(s) 749
BsiHKAI GWGCWC 2 cut(s) 665, 852
BsiSI CCGG 6 cut(s) 666, 693, 707, 721, 742, 777
BslI CCNNNNNNNGG 5 cut(s) 295, 699, 720, 741, 839
BsmAI GTCTC 2 cut(s) 274, 694
BsmI GAATGC 1 cut(s) 991
BsnI GGCC 3 cut(s) 93, 509, 679
Bso31I GGTCTC 1 cut(s) 694
Bsp1286I GDGCHC 2 cut(s) 665, 852
Bsp143I GATC 3 cut(s) 418, 934, 998
BspACI CCGC 4 cut(s) 552, 686, 991, 994
BspANI GGCC 3 cut(s) 93, 509, 679
BspCNI CTCAG 3 cut(s) 150, 424, 586
BspFNI CGCG 2 cut(s) 686, 991
BspLI GGNNCC 1 cut(s) 751
BspMI ACCTGC 1 cut(s) 379
BspT107I GGYRCC 1 cut(s) 749
BspTNI GGTCTC 1 cut(s) 694
BsrDI GCAATG 2 cut(s) 147, 282
BsrI ACTGG 5 cut(s) 300, 673, 680, 714, 751
BssECI CCNNGG 2 cut(s) 510, 834
BssMI GATC 3 cut(s) 418, 934, 998
BssNI GRCGYC 5 cut(s) 710, 750, 949, 971, 980
BssT1I CCWWGG 1 cut(s) 510
Bst4CI ACNGT 2 cut(s) 37, 838
Bst6I CTCTTC 3 cut(s) 559, 767, 774
BstACI GRCGYC 5 cut(s) 710, 750, 949, 971, 980
BstBAI YACGTR 1 cut(s) 886
BstC8I GCNNGC 1 cut(s) 386
BstDEI CTNAG 3 cut(s) 158, 411, 594
BstDSI CCRYGG 1 cut(s) 834
BstEII GGTNACC 1 cut(s) 668
BstF5I GGATG 1 cut(s) 644
BstFNI CGCG 2 cut(s) 686, 991
BstH2I RGCGCY 1 cut(s) 753
BstHHI GCGC 2 cut(s) 752, 991
BstKTI GATC 3 cut(s) 421, 937, 1001
BstMAI GTCTC 2 cut(s) 274, 694
BstMBI GATC 3 cut(s) 418, 934, 998
BstNSI RCATGY 2 cut(s) 357, 463
BstPAI GACNNNNGTC 1 cut(s) 702
BstPI GGTNACC 1 cut(s) 668
BstSLI GKGCMC 1 cut(s) 852
BstUI CGCG 2 cut(s) 686, 991
BstV1I GCAGC 1 cut(s) 166
BstV2I GAAGAC 1 cut(s) 36
BstX2I RGATCY 1 cut(s) 418
BstYI RGATCY 1 cut(s) 418
BsuRI GGCC 3 cut(s) 93, 509, 679
BtgI CCRYGG 1 cut(s) 834
BtsCI GGATG 1 cut(s) 644
BtsIMutI CAGTG 1 cut(s) 680
BveI ACCTGC 1 cut(s) 379
Cac8I GCNNGC 1 cut(s) 386
CfoI GCGC 2 cut(s) 752, 991
CseI GACGC 2 cut(s) 14, 699
Csp6I GTAC 5 cut(s) 110, 408, 463, 540, 619
CviAII CATG 6 cut(s) 89, 235, 354, 424, 460, 795
CviQI GTAC 5 cut(s) 110, 408, 463, 540, 619
DdeI CTNAG 3 cut(s) 158, 411, 594
DinI GGCGCC 1 cut(s) 751
DpnI GATC 3 cut(s) 420, 936, 1000
DpnII GATC 3 cut(s) 418, 934, 998
DrdI GACNNNNNNGTC 1 cut(s) 40
DseDI GACNNNNNNGTC 1 cut(s) 40
EaeI YGGCCR 2 cut(s) 91, 677
Eam1104I CTCTTC 3 cut(s) 559, 767, 774
EarI CTCTTC 3 cut(s) 559, 767, 774
EciI GGCGGA 1 cut(s) 1009
Eco130I CCWWGG 1 cut(s) 510
Eco147I AGGCCT 1 cut(s) 509
Eco31I GGTCTC 1 cut(s) 694
Eco32I GATATC 1 cut(s) 526
Eco72I CACGTG 1 cut(s) 886
Eco91I GGTNACC 1 cut(s) 668
EcoO65I GGTNACC 1 cut(s) 668
EcoRV GATATC 1 cut(s) 526
EcoT14I CCWWGG 1 cut(s) 510
EgeI GGCGCC 1 cut(s) 751
EheI GGCGCC 1 cut(s) 751
ErhI CCWWGG 1 cut(s) 510
FaeI CATG 6 cut(s) 92, 238, 357, 427, 463, 798
FalI AAGNNNNNCTT 2 cut(s) 902, 934
FatI CATG 6 cut(s) 88, 234, 353, 423, 459, 794
FauI CCCGC 1 cut(s) 545
Fnu4HI GCNGC 2 cut(s) 180, 992
FokI GGATG 1 cut(s) 631
Fsp4HI GCNGC 2 cut(s) 180, 992
FspBI CTAG 1 cut(s) 54
GlaI GCGC 2 cut(s) 751, 990
GluI GCNGC 2 cut(s) 180, 992
GsaI CCCAGC 3 cut(s) 305, 310, 757
HaeII RGCGCY 1 cut(s) 753
HaeIII GGCC 3 cut(s) 93, 509, 679
HapII CCGG 6 cut(s) 666, 693, 707, 721, 742, 777
HgaI GACGC 2 cut(s) 14, 699
HhaI GCGC 2 cut(s) 752, 991
Hin1I GRCGYC 5 cut(s) 710, 750, 949, 971, 980
Hin1II CATG 6 cut(s) 92, 238, 357, 427, 463, 798
Hin6I GCGC 2 cut(s) 750, 989
HinP1I GCGC 2 cut(s) 750, 989
HincII GTYRAC 1 cut(s) 969
HindII GTYRAC 1 cut(s) 969
HinfI GANTC 7 cut(s) 567, 612, 703, 724, 731, 786, 940
HpaII CCGG 6 cut(s) 666, 693, 707, 721, 742, 777
HphI GGTGA 4 cut(s) 439, 680, 707, 710
Hpy166II GTNNAC 5 cut(s) 112, 476, 850, 883, 969
Hpy188I TCNGA 5 cut(s) 159, 736, 791, 939, 1003
Hpy188III TCNNGA 1 cut(s) 728
Hpy8I GTNNAC 5 cut(s) 112, 476, 850, 883, 969
Hpy99I CGWCG 3 cut(s) 824, 954, 985
HpyCH4III ACNGT 2 cut(s) 37, 838
HpyCH4IV ACGT 6 cut(s) 108, 465, 885, 949, 971, 980
HpyCH4V TGCA 8 cut(s) 140, 182, 251, 394, 519, 587, 783, 850
HpyF3I CTNAG 3 cut(s) 158, 411, 594
HpySE526I ACGT 6 cut(s) 108, 465, 885, 949, 971, 980
Hsp92I GRCGYC 5 cut(s) 710, 750, 949, 971, 980
Hsp92II CATG 6 cut(s) 92, 238, 357, 427, 463, 798
HspAI GCGC 2 cut(s) 750, 989
KasI GGCGCC 1 cut(s) 749
Kzo9I GATC 3 cut(s) 418, 934, 998
LmnI GCTCC 2 cut(s) 668, 762
Lsp1109I GCAGC 1 cut(s) 166
MaeI CTAG 1 cut(s) 54
MaeII ACGT 6 cut(s) 108, 465, 885, 949, 971, 980
MaeIII GTNAC 6 cut(s) 19, 31, 668, 695, 716, 972
MalI GATC 3 cut(s) 420, 936, 1000
MboI GATC 3 cut(s) 418, 934, 998
MboII GAAGA 6 cut(s) 36, 351, 576, 754, 761, 922
MflI RGATCY 1 cut(s) 418
MhlI GDGCHC 2 cut(s) 665, 852
MlsI TGGCCA 2 cut(s) 93, 679
MluCI AATT 2 cut(s) 581, 601
MluNI TGGCCA 2 cut(s) 93, 679
Mly113I GGCGCC 1 cut(s) 750
MlyI GAGTC 3 cut(s) 576, 712, 949
MmeI TCCRAC 3 cut(s) 126, 313, 759
Mox20I TGGCCA 2 cut(s) 93, 679
MscI TGGCCA 2 cut(s) 93, 679
MseI TTAA 4 cut(s) 59, 456, 504, 891
Msp20I TGGCCA 2 cut(s) 93, 679
MspI CCGG 6 cut(s) 666, 693, 707, 721, 742, 777
Mva1269I GAATGC 1 cut(s) 991
MvnI CGCG 2 cut(s) 686, 991
NarI GGCGCC 1 cut(s) 750
NdeII GATC 3 cut(s) 418, 934, 998
NlaIII CATG 6 cut(s) 92, 238, 357, 427, 463, 798
NlaIV GGNNCC 1 cut(s) 751
NmuCI GTSAC 5 cut(s) 31, 668, 695, 716, 972
NspI RCATGY 2 cut(s) 357, 463
PaqCI CACCTGC 1 cut(s) 379
PceI AGGCCT 1 cut(s) 509
PciI ACATGT 1 cut(s) 459
PcsI WCGNNNNNNNCGW 3 cut(s) 905, 949, 977
PctI GAATGC 1 cut(s) 991
PfeI GAWTC 4 cut(s) 612, 724, 731, 786
PflMI CCANNNNNTGG 1 cut(s) 295
PkrI GCNGC 2 cut(s) 181, 993
PleI GAGTC 3 cut(s) 575, 711, 948
PluTI GGCGCC 1 cut(s) 753
PmaCI CACGTG 1 cut(s) 886
PmlI CACGTG 1 cut(s) 886
PpsI GAGTC 3 cut(s) 575, 711, 948
Ppu21I YACGTR 1 cut(s) 886
PscI ACATGT 1 cut(s) 459
PshAI GACNNNNGTC 1 cut(s) 702
PspCI CACGTG 1 cut(s) 886
PspEI GGTNACC 1 cut(s) 668
PspFI CCCAGC 3 cut(s) 301, 306, 753
PspN4I GGNNCC 1 cut(s) 751
PsuI RGATCY 1 cut(s) 418
RsaI GTAC 5 cut(s) 111, 409, 464, 541, 620
RsaNI GTAC 5 cut(s) 110, 408, 463, 540, 619
SaqAI TTAA 4 cut(s) 59, 456, 504, 891
SatI GCNGC 2 cut(s) 180, 992
Sau3AI GATC 3 cut(s) 418, 934, 998
ScaI AGTACT 1 cut(s) 409
SchI GAGTC 3 cut(s) 576, 712, 949
SduI GDGCHC 2 cut(s) 665, 852
SfoI GGCGCC 1 cut(s) 751
SmlI CTYRAG 1 cut(s) 373
SmoI CTYRAG 1 cut(s) 373
Sse9I AATT 2 cut(s) 581, 601
SseBI AGGCCT 1 cut(s) 509
SsiI CCGC 4 cut(s) 552, 686, 991, 994
SspDI GGCGCC 1 cut(s) 749
SspMI CTAG 1 cut(s) 54
StuI AGGCCT 1 cut(s) 509
StyI CCWWGG 1 cut(s) 510
TaaI ACNGT 2 cut(s) 37, 838
TaiI ACGT 6 cut(s) 111, 468, 888, 952, 974, 983
TaqI TCGA 3 cut(s) 557, 952, 983
TasI AATT 2 cut(s) 581, 601
TatI WGTACW 2 cut(s) 407, 618
TauI GCSGC 1 cut(s) 994
TfiI GAWTC 4 cut(s) 612, 724, 731, 786
Tru1I TTAA 4 cut(s) 59, 456, 504, 891
Tru9I TTAA 4 cut(s) 59, 456, 504, 891
TscAI CASTG 1 cut(s) 680
TseFI GTSAC 5 cut(s) 31, 668, 695, 716, 972
TseI GCWGC 1 cut(s) 179
Tsp45I GTSAC 5 cut(s) 31, 668, 695, 716, 972
TspDTI ATGAA 1 cut(s) 77
TspRI CASTG 1 cut(s) 680
Van91I CCANNNNNTGG 1 cut(s) 295
VneI GTGCAC 1 cut(s) 848
XapI RAATTY 2 cut(s) 581, 601
XceI RCATGY 2 cut(s) 357, 463
XspI CTAG 1 cut(s) 54
ZraI GACGTC 3 cut(s) 950, 972, 981
ZrmI AGTACT 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.