Rroxscaffold_3G00228190

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001684
Physical Location & Seq
Forward (+)
12560674 .. 12562182
1509 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00228190.1

Sequence Viewer

Length: 1509 bp
ATGAGACAACTAAACCAAATCCATGCTCTGCTGGTCAAAAATCCAAAACCCCAAGTTTTAAACCCCTGGCTGGGATATCTGACAAACTCTTCAGCACCACAAAATGCTCTTTTTCTCTATAACCAAATGCTCCACCACCCAACTTCCCATAACCACTACACATTTACTTATGCTCTAAAGGCATGCTGCTTGCTCCATTCACCCCATAAAGGCCAAGAAATCCAAGCCCACGTCACAAAATCCGGTCACATATCTGATACCTTCATCCAAAACTCTTTGCTCCACTTTTACGTAATCCTATCTGACATTGTTTCTGCTACCCGCGTTTTTGATTCAATACCCGTACCGGATGTTGTTTCCTGGACTTCGATGATTTCGGGTCTTTCCAAGTGTGGGTTTGTGGAGGAAGCCATTGTGAAGTTTATGTCCATGGACGTGAAACCTAATTCTACTACTCTTGTGACTGTTTTGTCTTCTTGTTCTACTTTAAGAGCTTTGAAATTTGGTAAAGCCGTTCATGGCCATTGTTTGAGGAACTTTCGGGAAAGAAATTTGATTTTGGACAATGCGGTGTTGGATTTCTATCTGAGATGTGGATCTTTGGCGAGTGCACGATACCTGTTTGTCAATATGCCCAAGAGAGATGTGGTTTCTTGGACTATCATGGTGGGTGGTTATGCACAAAGAGGTTTTTGTGAAGAGGCAGTAAAACTTTTCCAACAACTGCTGCAGGGTGGAGAAGCTGAGCCTAATGAGGCTACCATTGTCAATGTATTGTCAGCATGTTCTTCGATTTGTGCATTAAGTTCAGGCCAGCAGGTGCATTCCTATATCAGTACTCGACGAGATCTCATGGTGAATGGCAATGTGGGGAATGCTTTAATCAATATGTATGTGAAGTGTGGAAACTTGGAAATGGCTATCTCAGTTTTTCAGTCCCTGGAGCACAAAGACATCATTTCATGGAGTACCATAATATGCGGCATGGCCATGAATGGCCATGGCATACACTTGTTGCAGCTCTTTTCCAGTATGCTAGTCAATGGGGTGTCTCCTGATGGTGTAACCTTCCTCGGGTTGTTATCAGCATGCAGTCATGCTGGCCTGGTAAATCAAGGGTTGATGTTCTTCAATGCTATGAAGAAAGTTTACCGGATTGTCCCTGAAACACAGCATTATGCGTGCCTGGTCGATATGTATGGTCGAGCTGGGCTATTAGAGGAAGCAGAGGCTTTTATTAAAGAAATGCCAACAGAAGCTGATGGTCCTGTTTGGGGAGCGCTGCTTAATGCTTGTAAAATCTATGGGAATGAGGAGATGGTTGAGAGGATCAGAGAAGGCCTCCGCAATAGTGCAAGAGTGAGCACCGGAACTTATGCTCTGTTATCAAATGCTTATGCTAAACATGATAGGTGGGATGATTCTAATAAGGTTCGTGATGAAATGAGAGAGATGGGGTTGAAGGCACCACCAGGTCGTAGTTGGATTGAAATTGGTCCGTCCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

502

Amino Acids

55.67

Weight (kDa)

7.91

Isoelectric Point (pI)

41.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 116 - 160 1e-05 PPR repeat family
PPR PF01535 217 - 244 7.6e-06 PPR repeat
PPR_2 PF13041 317 - 365 4.8e-07 PPR repeat family
E_motif PF20431 445 - 497 4e-10 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 808
AasI GACNNNNNNGTC 1 cut(s) 469
Acc36I ACCTGC 1 cut(s) 808
AccB1I GGYRCC 1 cut(s) 1465
AccII CGCG 1 cut(s) 324
AciI CCGC 4 cut(s) 322, 569, 981, 1345
AclWI GGATC 2 cut(s) 604, 1337
AcoI YGGCCR 3 cut(s) 520, 987, 997
AcsI RAATTY 2 cut(s) 500, 550
AcuI CTGAAG 1 cut(s) 75
AfaI GTAC 3 cut(s) 345, 838, 970
AfeI AGCGCT 1 cut(s) 1281
AfiI CCNNNNNNNGG 6 cut(s) 70, 71, 209, 393, 1074, 1274
AgsI TTSAA 5 cut(s) 336, 499, 1132, 1462, 1490
AjiI CACGTC 2 cut(s) 232, 436
AjnI CCWGG 6 cut(s) 65, 359, 939, 1104, 1185, 1471
AluBI AGCT 5 cut(s) 494, 743, 1021, 1208, 1259
AluI AGCT 5 cut(s) 494, 743, 1021, 1208, 1259
Alw21I GWGCWC 3 cut(s) 613, 948, 1367
Alw26I GTCTC 1 cut(s) 1056
Alw44I GTGCAC 1 cut(s) 609
AlwI GGATC 2 cut(s) 604, 1337
AlwNI CAGNNNCTG 2 cut(s) 940, 1259
Ama87I CYCGRG 1 cut(s) 1073
Aor51HI AGCGCT 1 cut(s) 1281
AoxI GGCC 7 cut(s) 211, 520, 811, 987, 997, 1102, 1339
ApaLI GTGCAC 1 cut(s) 609
ApeKI GCWGC 4 cut(s) 186, 727, 1018, 1282
ApoI RAATTY 2 cut(s) 500, 550
AspLEI GCGC 1 cut(s) 1282
AspS9I GGNCC 2 cut(s) 1265, 1496
AsuHPI GGTGA 2 cut(s) 192, 868
AvaI CYCGRG 1 cut(s) 1073
AvaII GGWCC 2 cut(s) 1265, 1496
BaeGI GKGCMC 1 cut(s) 613
BalI TGGCCA 3 cut(s) 522, 989, 999
BanI GGYRCC 1 cut(s) 1465
BarI GAAGNNNNNNTAC 2 cut(s) 1133, 1165
BbsI GAAGAC 1 cut(s) 465
Bbv12I GWGCWC 3 cut(s) 613, 948, 1367
BbvI GCAGC 4 cut(s) 173, 714, 1030, 1269
BccI CCATC 4 cut(s) 1052, 1256, 1312, 1447
BceAI ACGGC 1 cut(s) 497
BcgI CGANNNNNNTGC 2 cut(s) 771, 805
BciT130I CCWGG 6 cut(s) 67, 361, 941, 1106, 1187, 1473
BcoDI GTCTC 1 cut(s) 1056
BfaI CTAG 1 cut(s) 1037
BfmI CTRYAG 1 cut(s) 728
BfoI RGCGCY 1 cut(s) 1283
BfuAI ACCTGC 1 cut(s) 808
BglII AGATCT 1 cut(s) 847
BisI GCNGC 5 cut(s) 187, 728, 982, 1019, 1283
BlpI GCTNAGC 1 cut(s) 744
BlsI GCNGC 5 cut(s) 188, 729, 983, 1020, 1284
BmcAI AGTACT 1 cut(s) 838
Bme1390I CCNGG 6 cut(s) 67, 361, 941, 1106, 1187, 1473
Bme18I GGWCC 2 cut(s) 1265, 1496
BmeT110I CYCGRG 1 cut(s) 1073
BmgBI CACGTC 2 cut(s) 232, 436
BmgT120I GGNCC 2 cut(s) 1265, 1496
BmiI GGNNCC 1 cut(s) 1467
BmrFI CCNGG 6 cut(s) 67, 361, 941, 1106, 1187, 1473
BpiI GAAGAC 1 cut(s) 465
BplI GAGNNNNNCTC 2 cut(s) 1326, 1358
BpmI CTGGAG 1 cut(s) 962
Bpu1102I GCTNAGC 1 cut(s) 744
BsaAI YACGTR 1 cut(s) 292
BsaBI GATNNNNATC 2 cut(s) 582, 595
BsaJI CCNNGG 5 cut(s) 65, 429, 939, 1000, 1072
BsaWI WCCGGW 4 cut(s) 242, 346, 1152, 1367
Bsc4I CCNNNNNNNGG 6 cut(s) 70, 71, 209, 393, 1074, 1274
Bse1I ACTGG 1 cut(s) 1029
Bse3DI GCAATG 1 cut(s) 871
Bse8I GATNNNNATC 2 cut(s) 582, 595
BseBI CCWGG 6 cut(s) 67, 361, 941, 1106, 1187, 1473
BseDI CCNNGG 5 cut(s) 65, 429, 939, 1000, 1072
BseGI GGATG 3 cut(s) 264, 355, 1423
BseJI GATNNNNATC 2 cut(s) 582, 595
BseLI CCNNNNNNNGG 6 cut(s) 70, 71, 209, 393, 1074, 1274
BseMI GCAATG 1 cut(s) 871
BseMII CTCAG 3 cut(s) 578, 735, 939
BseNI ACTGG 1 cut(s) 1029
BseRI GAGGAG 1 cut(s) 1328
BseSI GKGCMC 1 cut(s) 613
BseXI GCAGC 4 cut(s) 173, 714, 1030, 1269
BseYI CCCAGC 2 cut(s) 70, 1208
Bsh1236I CGCG 1 cut(s) 324
BshFI GGCC 7 cut(s) 213, 522, 813, 989, 999, 1104, 1341
BshNI GGYRCC 1 cut(s) 1465
BsiHKAI GWGCWC 3 cut(s) 613, 948, 1367
BsiHKCI CYCGRG 1 cut(s) 1073
BsiSI CCGG 4 cut(s) 243, 347, 1153, 1368
BslFI GGGAC 2 cut(s) 922, 1145
BslI CCNNNNNNNGG 6 cut(s) 70, 71, 209, 393, 1074, 1274
BsmAI GTCTC 1 cut(s) 1056
BsmFI GGGAC 2 cut(s) 922, 1145
BsmI GAATGC 2 cut(s) 823, 880
BsnI GGCC 7 cut(s) 213, 522, 813, 989, 999, 1104, 1341
BsoBI CYCGRG 1 cut(s) 1073
Bsp1286I GDGCHC 3 cut(s) 613, 948, 1367
Bsp143I GATC 3 cut(s) 596, 847, 1329
Bsp1720I GCTNAGC 1 cut(s) 744
Bsp19I CCATGG 2 cut(s) 429, 1000
BspACI CCGC 4 cut(s) 322, 569, 981, 1345
BspANI GGCC 7 cut(s) 213, 522, 813, 989, 999, 1104, 1341
BspCNI CTCAG 3 cut(s) 579, 736, 938
BspFNI CGCG 1 cut(s) 324
BspLI GGNNCC 1 cut(s) 1467
BspMAI CTGCAG 1 cut(s) 732
BspMI ACCTGC 1 cut(s) 808
BspPI GGATC 2 cut(s) 604, 1337
BspT107I GGYRCC 1 cut(s) 1465
BsrDI GCAATG 1 cut(s) 871
BsrI ACTGG 1 cut(s) 1029
BssECI CCNNGG 5 cut(s) 65, 429, 939, 1000, 1072
BssMI GATC 3 cut(s) 596, 847, 1329
BssT1I CCWWGG 2 cut(s) 429, 1000
Bst2UI CCWGG 6 cut(s) 67, 361, 941, 1106, 1187, 1473
Bst4CI ACNGT 1 cut(s) 466
Bst6I CTCTTC 2 cut(s) 94, 693
BstBAI YACGTR 1 cut(s) 292
BstC8I GCNNGC 6 cut(s) 184, 191, 815, 1090, 1102, 1183
BstDEI CTNAG 3 cut(s) 587, 744, 925
BstDSI CCRYGG 2 cut(s) 429, 1000
BstF5I GGATG 3 cut(s) 264, 355, 1423
BstFNI CGCG 1 cut(s) 324
BstH2I RGCGCY 1 cut(s) 1283
BstHHI GCGC 1 cut(s) 1282
BstKTI GATC 3 cut(s) 599, 850, 1332
BstMAI GTCTC 1 cut(s) 1056
BstMBI GATC 3 cut(s) 596, 847, 1329
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstNI CCWGG 6 cut(s) 67, 361, 941, 1106, 1187, 1473
BstNSI RCATGY 3 cut(s) 186, 786, 1092
BstSCI CCNGG 6 cut(s) 65, 359, 939, 1104, 1185, 1471
BstSFI CTRYAG 1 cut(s) 728
BstSLI GKGCMC 1 cut(s) 613
BstSNI TACGTA 1 cut(s) 292
BstUI CGCG 1 cut(s) 324
BstV1I GCAGC 4 cut(s) 173, 714, 1030, 1269
BstV2I GAAGAC 1 cut(s) 465
BstX2I RGATCY 2 cut(s) 596, 847
BstYI RGATCY 2 cut(s) 596, 847
BsuRI GGCC 7 cut(s) 213, 522, 813, 989, 999, 1104, 1341
BtgI CCRYGG 2 cut(s) 429, 1000
BtrI CACGTC 2 cut(s) 232, 436
BtsCI GGATG 3 cut(s) 264, 355, 1423
BveI ACCTGC 1 cut(s) 808
Cac8I GCNNGC 6 cut(s) 184, 191, 815, 1090, 1102, 1183
CaiI CAGNNNCTG 2 cut(s) 940, 1259
CfoI GCGC 1 cut(s) 1282
Cfr13I GGNCC 2 cut(s) 1265, 1496
CsiI ACCWGGT 1 cut(s) 1471
Csp6I GTAC 3 cut(s) 344, 837, 969
CviQI GTAC 3 cut(s) 344, 837, 969
DdeI CTNAG 3 cut(s) 587, 744, 925
DpnI GATC 3 cut(s) 598, 849, 1331
DpnII GATC 3 cut(s) 596, 847, 1329
DraI TTTAAA 1 cut(s) 60
DrdI GACNNNNNNGTC 1 cut(s) 469
DseDI GACNNNNNNGTC 1 cut(s) 469
EaeI YGGCCR 3 cut(s) 520, 987, 997
Eam1104I CTCTTC 2 cut(s) 94, 693
EarI CTCTTC 2 cut(s) 94, 693
Eco105I TACGTA 1 cut(s) 292
Eco130I CCWWGG 2 cut(s) 429, 1000
Eco147I AGGCCT 1 cut(s) 1341
Eco32I GATATC 1 cut(s) 77
Eco47I GGWCC 2 cut(s) 1265, 1496
Eco47III AGCGCT 1 cut(s) 1281
Eco57I CTGAAG 1 cut(s) 75
Eco88I CYCGRG 1 cut(s) 1073
EcoRII CCWGG 6 cut(s) 65, 359, 939, 1104, 1185, 1471
EcoRV GATATC 1 cut(s) 77
EcoT14I CCWWGG 2 cut(s) 429, 1000
ErhI CCWWGG 2 cut(s) 429, 1000
FaqI GGGAC 2 cut(s) 922, 1145
FauI CCCGC 1 cut(s) 329
Fnu4HI GCNGC 5 cut(s) 187, 728, 982, 1019, 1283
FokI GGATG 3 cut(s) 251, 362, 1430
Fsp4HI GCNGC 5 cut(s) 187, 728, 982, 1019, 1283
FspBI CTAG 1 cut(s) 1037
GlaI GCGC 1 cut(s) 1281
GluI GCNGC 5 cut(s) 187, 728, 982, 1019, 1283
GsaI CCCAGC 2 cut(s) 74, 1212
GsuI CTGGAG 1 cut(s) 962
HaeII RGCGCY 1 cut(s) 1283
HaeIII GGCC 7 cut(s) 213, 522, 813, 989, 999, 1104, 1341
HapII CCGG 4 cut(s) 243, 347, 1153, 1368
HhaI GCGC 1 cut(s) 1282
Hin6I GCGC 1 cut(s) 1280
HinP1I GCGC 1 cut(s) 1280
HinfI GANTC 2 cut(s) 332, 1421
HpaII CCGG 4 cut(s) 243, 347, 1153, 1368
HphI GGTGA 2 cut(s) 192, 868
Hpy166II GTNNAC 2 cut(s) 611, 1150
Hpy188I TCNGA 5 cut(s) 81, 256, 304, 588, 1334
Hpy188III TCNNGA 3 cut(s) 542, 1055, 1436
Hpy8I GTNNAC 2 cut(s) 611, 1150
Hpy99I CGWCG 1 cut(s) 846
HpyAV CCTTC 4 cut(s) 271, 1078, 1331, 1456
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4IV ACGT 3 cut(s) 231, 291, 435
HpyCH4V TGCA 8 cut(s) 611, 680, 730, 800, 823, 1018, 1092, 1355
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
HpyF3I CTNAG 3 cut(s) 587, 744, 925
HpySE526I ACGT 3 cut(s) 231, 291, 435
HspAI GCGC 1 cut(s) 1280
Kzo9I GATC 3 cut(s) 596, 847, 1329
LmnI GCTCC 5 cut(s) 135, 198, 285, 943, 1277
Lsp1109I GCAGC 4 cut(s) 173, 714, 1030, 1269
MabI ACCWGGT 1 cut(s) 1471
MaeI CTAG 1 cut(s) 1037
MaeII ACGT 3 cut(s) 231, 291, 435
MaeIII GTNAC 4 cut(s) 232, 245, 460, 1063
MalI GATC 3 cut(s) 598, 849, 1331
MboI GATC 3 cut(s) 596, 847, 1329
MboII GAAGA 6 cut(s) 81, 465, 710, 780, 1120, 1153
MflI RGATCY 2 cut(s) 596, 847
MhlI GDGCHC 3 cut(s) 613, 948, 1367
MlsI TGGCCA 3 cut(s) 522, 989, 999
MluCI AATT 4 cut(s) 445, 500, 550, 1491
MluNI TGGCCA 3 cut(s) 522, 989, 999
MmeI TCCRAC 3 cut(s) 555, 742, 1463
Mox20I TGGCCA 3 cut(s) 522, 989, 999
MscI TGGCCA 3 cut(s) 522, 989, 999
MseI TTAA 6 cut(s) 59, 488, 803, 881, 1239, 1287
MslI CAYNNNNRTG 2 cut(s) 434, 989
Msp20I TGGCCA 3 cut(s) 522, 989, 999
MspI CCGG 4 cut(s) 243, 347, 1153, 1368
MspR9I CCNGG 6 cut(s) 67, 361, 941, 1106, 1187, 1473
Mva1269I GAATGC 2 cut(s) 823, 880
MvaI CCWGG 6 cut(s) 67, 361, 941, 1106, 1187, 1473
MvnI CGCG 1 cut(s) 324
MwoI GCNNNNNNNGC 1 cut(s) 179
NcoI CCATGG 2 cut(s) 429, 1000
NdeII GATC 3 cut(s) 596, 847, 1329
NlaIV GGNNCC 1 cut(s) 1467
NmuCI GTSAC 3 cut(s) 232, 245, 460
NspI RCATGY 3 cut(s) 186, 786, 1092
PaeI GCATGC 2 cut(s) 186, 1092
PaqCI CACCTGC 1 cut(s) 808
PceI AGGCCT 1 cut(s) 1341
PctI GAATGC 2 cut(s) 823, 880
PfeI GAWTC 2 cut(s) 332, 1421
PfoI TCCNGGA 1 cut(s) 359
PkrI GCNGC 5 cut(s) 188, 729, 983, 1020, 1284
Ppu21I YACGTR 1 cut(s) 292
Psp6I CCWGG 6 cut(s) 65, 359, 939, 1104, 1185, 1471
PspFI CCCAGC 2 cut(s) 70, 1208
PspGI CCWGG 6 cut(s) 65, 359, 939, 1104, 1185, 1471
PspN4I GGNNCC 1 cut(s) 1467
PspPI GGNCC 2 cut(s) 1265, 1496
PstI CTGCAG 1 cut(s) 732
PstNI CAGNNNCTG 2 cut(s) 940, 1259
PsuI RGATCY 2 cut(s) 596, 847
RsaI GTAC 3 cut(s) 345, 838, 970
RsaNI GTAC 3 cut(s) 344, 837, 969
RseI CAYNNNNRTG 2 cut(s) 434, 989
SaqAI TTAA 6 cut(s) 59, 488, 803, 881, 1239, 1287
SatI GCNGC 5 cut(s) 187, 728, 982, 1019, 1283
Sau3AI GATC 3 cut(s) 596, 847, 1329
Sau96I GGNCC 2 cut(s) 1265, 1496
ScaI AGTACT 1 cut(s) 838
ScrFI CCNGG 6 cut(s) 67, 361, 941, 1106, 1187, 1473
SduI GDGCHC 3 cut(s) 613, 948, 1367
SexAI ACCWGGT 1 cut(s) 1471
SfcI CTRYAG 1 cut(s) 728
SinI GGWCC 2 cut(s) 1265, 1496
SmiMI CAYNNNNRTG 2 cut(s) 434, 989
SnaBI TACGTA 1 cut(s) 292
SphI GCATGC 2 cut(s) 186, 1092
Sse9I AATT 4 cut(s) 445, 500, 550, 1491
SseBI AGGCCT 1 cut(s) 1341
SsiI CCGC 4 cut(s) 322, 569, 981, 1345
SspMI CTAG 1 cut(s) 1037
StuI AGGCCT 1 cut(s) 1341
StyD4I CCNGG 6 cut(s) 65, 359, 939, 1104, 1185, 1471
StyI CCWWGG 2 cut(s) 429, 1000
TaaI ACNGT 1 cut(s) 466
TaiI ACGT 3 cut(s) 234, 294, 438
TaqI TCGA 5 cut(s) 368, 791, 841, 1191, 1204
TasI AATT 4 cut(s) 445, 500, 550, 1491
TatI WGTACW 1 cut(s) 836
TauI GCSGC 1 cut(s) 984
TfiI GAWTC 2 cut(s) 332, 1421
Tru1I TTAA 6 cut(s) 59, 488, 803, 881, 1239, 1287
Tru9I TTAA 6 cut(s) 59, 488, 803, 881, 1239, 1287
TseFI GTSAC 3 cut(s) 232, 245, 460
TseI GCWGC 4 cut(s) 186, 727, 1018, 1282
Tsp45I GTSAC 3 cut(s) 232, 245, 460
TspDTI ATGAA 6 cut(s) 253, 506, 951, 1007, 1154, 1455
TspGWI ACGGA 1 cut(s) 1488
VneI GTGCAC 1 cut(s) 609
VpaK11BI GGWCC 2 cut(s) 1265, 1496
XapI RAATTY 2 cut(s) 500, 550
XceI RCATGY 3 cut(s) 186, 786, 1092
XcmI CCANNNNNNNNNTGG 2 cut(s) 643, 1479
XspI CTAG 1 cut(s) 1037
ZrmI AGTACT 1 cut(s) 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.