FvH4_6g28140

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000757
Physical Location & Seq
Forward (+)
21665145 .. 21666441
1297 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g28140.t1

Sequence Viewer

Length: 1125 bp
ATGCGAGCAGGTACTTTACTCTGTATTCCATATCATCAGCTTCATCACCATCATCCTTACCTATTTCAGACCATCGTCGAGTCCCGAGATCCTATGCAGAACCATCTACTGCCCAGTTCGCATCCTATTGTTCTTCCAGTTCCCCGTTCCCAACGTATTGTTTTTCCAGTCCCTGGCATAATCAATCAGGTTTGGCACAAGAATTTGGGGTACGAGTTCAAGAAAATCCGCAACTTACTGTTCTGGCCCGACGAGAACCATGCCCGTGACCGCTGCTTTCTTGCTTCTATAGACACCGAATTCCCCGGTGTACTTTCTACATCCGACAAGGGCCGTCTCCAAGATCCACACTACAATTATGAGCAGATGAAGTACAATGTGAACGCCACCAACATAATCCAACTGGGTTTGACTCTCTCGGACAGATACGGAAACTTCTACACATGGGAGTTCAATTTCAGCGACTTTGATATCGAAACTGATGATCACAACGACGCCTCAATTGAGCTTCTCAAGACTCACGGGATCGATTTCGAGAGAAACAAGAAGGAAGGCATTCCTTCTTCTGAGTTTGCAGAGTTGTGTCGGCGCTCTGGGCTGGTAGGAAACAATTCACAGATACCTTTGACATGGGTGGGTTTCCAGAGTTCTTATGATTTTGGCTTCTTCAGCAAGATTCTCACTGGAAAAAATTTGCCAGACGACATGGAAGTGTTCATGAGATTCGTAAAGATGTGCTTTGGGCCTAGCATGTTTGACATTAAACACATGATTACGAGTCTTGGTGCTGGACTTTATGGAGGTTTGGAGAAGGTGGCAGAGATGCTCGGGGTTGCTTGGGCAGCCGGGAAGAGTCACACGGCTGGTTCTGACAGTCTTTTGACATTGAAAACCTTTATCGAGCTTGTATATGTGTGTTTGGAGCCTGCATATTCTAATACCAGCTGCATTGAGACATTGCAGAATTTTACAATGAATATGAGTTTTGCGAATGCCATGGCTCCTTGTGATATTGATAAGGCCAAGGAAGTCTTTGAGTTTCTTATAAGAGGGGAGGAAGAAAGAGAGAAACAGAACTATGATAAGTTGTATAGTTGTAAAGGACTCAAGGGGCTTTGTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1

Protein Analysis

375

Amino Acids

42.76

Weight (kDa)

5.87

Isoelectric Point (pI)

35.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 92 - 187 2.5e-10 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1046
AccB7I CCANNNNNTGG 1 cut(s) 173
AciI CCGC 2 cut(s) 229, 271
AclWI GGATC 3 cut(s) 83, 338, 533
AcsI RAATTY 4 cut(s) 202, 299, 691, 964
AcuI CTGAAG 1 cut(s) 652
AcyI GRCGYC 1 cut(s) 495
AfaI GTAC 4 cut(s) 13, 212, 312, 374
AfiI CCNNNNNNNGG 1 cut(s) 173
AgsI TTSAA 3 cut(s) 220, 454, 889
AjnI CCWGG 1 cut(s) 172
AluBI AGCT 4 cut(s) 40, 508, 904, 945
AluI AGCT 4 cut(s) 40, 508, 904, 945
Alw26I GTCTC 2 cut(s) 341, 947
AlwI GGATC 3 cut(s) 83, 338, 533
AlwNI CAGNNNCTG 1 cut(s) 173
Ama87I CYCGRG 2 cut(s) 84, 827
AoxI GGCC 4 cut(s) 245, 331, 743, 1020
ApeKI GCWGC 3 cut(s) 273, 842, 945
ApoI RAATTY 4 cut(s) 202, 299, 691, 964
Asp700I GAANNNNTTC 2 cut(s) 555, 610
AspLEI GCGC 1 cut(s) 591
AspS9I GGNCC 3 cut(s) 246, 331, 743
AsuC2I CCSGG 2 cut(s) 306, 847
AsuHPI GGTGA 1 cut(s) 38
AvaI CYCGRG 2 cut(s) 84, 827
BbvI GCAGC 3 cut(s) 260, 854, 932
BccI CCATC 3 cut(s) 57, 80, 111
BceAI ACGGC 2 cut(s) 318, 876
BcgI CGANNNNNNTGC 2 cut(s) 242, 276
BciT130I CCWGG 1 cut(s) 174
BclI TGATCA 1 cut(s) 484
BcnI CCSGG 2 cut(s) 306, 847
BcoDI GTCTC 2 cut(s) 341, 947
BfaI CTAG 1 cut(s) 747
BfmI CTRYAG 1 cut(s) 288
BfoI RGCGCY 1 cut(s) 592
BisI GCNGC 3 cut(s) 274, 843, 946
BlsI GCNGC 3 cut(s) 275, 844, 947
Bme1390I CCNGG 3 cut(s) 174, 306, 847
BmeT110I CYCGRG 2 cut(s) 84, 827
BmgT120I GGNCC 3 cut(s) 246, 331, 743
BmiI GGNNCC 2 cut(s) 924, 1002
BmrFI CCNGG 3 cut(s) 174, 306, 847
BmrI ACTGGG 2 cut(s) 108, 413
BmsI GCATC 2 cut(s) 130, 813
BmuI ACTGGG 2 cut(s) 108, 413
BoxI GACNNNNGTC 1 cut(s) 74
BpuEI CTTGAG 2 cut(s) 497, 1091
BpuMI CCSGG 2 cut(s) 306, 847
Bsa29I ATCGAT 1 cut(s) 528
BsaHI GRCGYC 1 cut(s) 495
BsaJI CCNNGG 4 cut(s) 172, 304, 996, 1023
Bsc4I CCNNNNNNNGG 1 cut(s) 173
Bse1I ACTGG 5 cut(s) 114, 137, 167, 408, 688
Bse3DI GCAATG 1 cut(s) 956
BseBI CCWGG 1 cut(s) 174
BseCI ATCGAT 1 cut(s) 528
BseDI CCNNGG 4 cut(s) 172, 304, 996, 1023
BseGI GGATG 3 cut(s) 52, 121, 320
BseLI CCNNNNNNNGG 1 cut(s) 173
BseMI GCAATG 1 cut(s) 956
BseMII CTCAG 1 cut(s) 558
BseNI ACTGG 5 cut(s) 114, 137, 167, 408, 688
BseXI GCAGC 3 cut(s) 260, 854, 932
BshFI GGCC 4 cut(s) 247, 333, 745, 1022
BshVI ATCGAT 1 cut(s) 528
BsiHKCI CYCGRG 2 cut(s) 84, 827
BsiSI CCGG 2 cut(s) 306, 846
BslFI GGGAC 2 cut(s) 67, 155
BslI CCNNNNNNNGG 1 cut(s) 173
BsmAI GTCTC 2 cut(s) 341, 947
BsmBI CGTCTC 1 cut(s) 341
BsmFI GGGAC 2 cut(s) 67, 155
BsmI GAATGC 2 cut(s) 555, 997
BsnI GGCC 4 cut(s) 247, 333, 745, 1022
BsoBI CYCGRG 2 cut(s) 84, 827
Bsp143I GATC 4 cut(s) 88, 343, 484, 525
Bsp19I CCATGG 1 cut(s) 996
BspACI CCGC 2 cut(s) 229, 271
BspANI GGCC 4 cut(s) 247, 333, 745, 1022
BspCNI CTCAG 1 cut(s) 559
BspDI ATCGAT 1 cut(s) 528
BspHI TCATGA 1 cut(s) 717
BspLI GGNNCC 2 cut(s) 924, 1002
BspPI GGATC 3 cut(s) 83, 338, 533
BsrDI GCAATG 1 cut(s) 956
BsrI ACTGG 5 cut(s) 114, 137, 167, 408, 688
BssECI CCNNGG 4 cut(s) 172, 304, 996, 1023
BssMI GATC 4 cut(s) 88, 343, 484, 525
BssNI GRCGYC 1 cut(s) 495
BssT1I CCWWGG 2 cut(s) 996, 1023
Bst2UI CCWGG 1 cut(s) 174
Bst4CI ACNGT 2 cut(s) 240, 875
Bst6I CTCTTC 1 cut(s) 845
BstACI GRCGYC 1 cut(s) 495
BstC8I GCNNGC 2 cut(s) 6, 927
BstDEI CTNAG 1 cut(s) 567
BstDSI CCRYGG 1 cut(s) 996
BstF5I GGATG 3 cut(s) 52, 121, 320
BstH2I RGCGCY 1 cut(s) 592
BstHHI GCGC 1 cut(s) 591
BstKTI GATC 4 cut(s) 91, 346, 487, 528
BstMAI GTCTC 2 cut(s) 341, 947
BstMBI GATC 4 cut(s) 88, 343, 484, 525
BstMWI GCNNNNNNNGC 4 cut(s) 118, 595, 669, 842
BstNI CCWGG 1 cut(s) 174
BstNSI RCATGY 1 cut(s) 754
BstPAI GACNNNNGTC 1 cut(s) 74
BstSCI CCNGG 3 cut(s) 172, 304, 845
BstSFI CTRYAG 1 cut(s) 288
BstV1I GCAGC 3 cut(s) 260, 854, 932
BstX2I RGATCY 2 cut(s) 88, 343
BstYI RGATCY 2 cut(s) 88, 343
Bsu15I ATCGAT 1 cut(s) 528
BsuRI GGCC 4 cut(s) 247, 333, 745, 1022
BsuTUI ATCGAT 1 cut(s) 528
BtgI CCRYGG 1 cut(s) 996
BtsCI GGATG 3 cut(s) 52, 121, 320
BtsIMutI CAGTG 1 cut(s) 681
Cac8I GCNNGC 2 cut(s) 6, 927
CaiI CAGNNNCTG 1 cut(s) 173
CciI TCATGA 1 cut(s) 717
CfoI GCGC 1 cut(s) 591
Cfr13I GGNCC 3 cut(s) 246, 331, 743
ClaI ATCGAT 1 cut(s) 528
CseI GACGC 1 cut(s) 503
Csp6I GTAC 4 cut(s) 12, 211, 311, 373
CviAII CATG 8 cut(s) 260, 444, 630, 706, 718, 751, 769, 997
CviQI GTAC 4 cut(s) 12, 211, 311, 373
DdeI CTNAG 1 cut(s) 567
DpnI GATC 4 cut(s) 90, 345, 486, 527
DpnII GATC 4 cut(s) 88, 343, 484, 525
Eam1104I CTCTTC 1 cut(s) 845
EarI CTCTTC 1 cut(s) 845
Eco130I CCWWGG 2 cut(s) 996, 1023
Eco32I GATATC 1 cut(s) 472
Eco57I CTGAAG 1 cut(s) 652
Eco88I CYCGRG 2 cut(s) 84, 827
EcoRI GAATTC 1 cut(s) 299
EcoRII CCWGG 1 cut(s) 172
EcoRV GATATC 1 cut(s) 472
EcoT14I CCWWGG 2 cut(s) 996, 1023
ErhI CCWWGG 2 cut(s) 996, 1023
Esp3I CGTCTC 1 cut(s) 341
FaeI CATG 8 cut(s) 263, 447, 633, 709, 721, 754, 772, 1000
FalI AAGNNNNNCTT 4 cut(s) 722, 754, 1016, 1048
FaqI GGGAC 2 cut(s) 67, 155
FatI CATG 8 cut(s) 259, 443, 629, 705, 717, 750, 768, 996
FbaI TGATCA 1 cut(s) 484
Fnu4HI GCNGC 3 cut(s) 274, 843, 946
FokI GGATG 3 cut(s) 39, 108, 307
Fsp4HI GCNGC 3 cut(s) 274, 843, 946
FspBI CTAG 1 cut(s) 747
GlaI GCGC 1 cut(s) 590
GluI GCNGC 3 cut(s) 274, 843, 946
HaeII RGCGCY 1 cut(s) 592
HaeIII GGCC 4 cut(s) 247, 333, 745, 1022
HapII CCGG 2 cut(s) 306, 846
HgaI GACGC 1 cut(s) 503
HhaI GCGC 1 cut(s) 591
Hin1I GRCGYC 1 cut(s) 495
Hin1II CATG 8 cut(s) 263, 447, 633, 709, 721, 754, 772, 1000
Hin6I GCGC 1 cut(s) 589
HinP1I GCGC 1 cut(s) 589
HinfI GANTC 8 cut(s) 80, 412, 517, 676, 723, 778, 853, 1104
HpaII CCGG 2 cut(s) 306, 846
HphI GGTGA 1 cut(s) 38
Hpy166II GTNNAC 2 cut(s) 311, 382
Hpy188I TCNGA 5 cut(s) 69, 325, 421, 568, 871
Hpy188III TCNNGA 6 cut(s) 84, 220, 514, 535, 643, 718
Hpy8I GTNNAC 2 cut(s) 311, 382
Hpy99I CGWCG 3 cut(s) 80, 254, 497
HpyAV CCTTC 4 cut(s) 541, 545, 570, 805
HpyCH4III ACNGT 2 cut(s) 240, 875
HpyCH4IV ACGT 1 cut(s) 154
HpyCH4V TGCA 5 cut(s) 97, 575, 929, 948, 961
HpyF10VI GCNNNNNNNGC 4 cut(s) 118, 595, 669, 842
HpyF3I CTNAG 1 cut(s) 567
HpySE526I ACGT 1 cut(s) 154
Hsp92I GRCGYC 1 cut(s) 495
Hsp92II CATG 8 cut(s) 263, 447, 633, 709, 721, 754, 772, 1000
HspAI GCGC 1 cut(s) 589
Ksp22I TGATCA 1 cut(s) 484
Kzo9I GATC 4 cut(s) 88, 343, 484, 525
LmnI GCTCC 2 cut(s) 922, 1006
Lsp1109I GCAGC 3 cut(s) 260, 854, 932
LweI GCATC 2 cut(s) 130, 813
MaeI CTAG 1 cut(s) 747
MaeII ACGT 1 cut(s) 154
MaeIII GTNAC 2 cut(s) 266, 854
MalI GATC 4 cut(s) 90, 345, 486, 527
MboI GATC 4 cut(s) 88, 343, 484, 525
MboII GAAGA 5 cut(s) 125, 555, 658, 862, 1070
MfeI CAATTG 1 cut(s) 501
MflI RGATCY 2 cut(s) 88, 343
MluCI AATT 8 cut(s) 202, 299, 355, 454, 501, 610, 691, 964
MlyI GAGTC 6 cut(s) 89, 406, 511, 787, 862, 1098
MmeI TCCRAC 2 cut(s) 348, 424
MnlI CCTC 4 cut(s) 508, 794, 1043, 1048
MroXI GAANNNNTTC 2 cut(s) 555, 610
MseI TTAA 1 cut(s) 762
MslI CAYNNNNRTG 2 cut(s) 264, 710
MspA1I CMGCKG 2 cut(s) 273, 945
MspI CCGG 2 cut(s) 306, 846
MspR9I CCNGG 3 cut(s) 174, 306, 847
MunI CAATTG 1 cut(s) 501
Mva1269I GAATGC 2 cut(s) 555, 997
MvaI CCWGG 1 cut(s) 174
MwoI GCNNNNNNNGC 4 cut(s) 118, 595, 669, 842
NciI CCSGG 2 cut(s) 306, 847
NcoI CCATGG 1 cut(s) 996
NdeII GATC 4 cut(s) 88, 343, 484, 525
NlaIII CATG 8 cut(s) 263, 447, 633, 709, 721, 754, 772, 1000
NlaIV GGNNCC 2 cut(s) 924, 1002
NmuCI GTSAC 2 cut(s) 266, 854
NspI RCATGY 1 cut(s) 754
PagI TCATGA 1 cut(s) 717
PctI GAATGC 2 cut(s) 555, 997
PdmI GAANNNNTTC 2 cut(s) 555, 610
PfeI GAWTC 2 cut(s) 676, 723
PflMI CCANNNNNTGG 1 cut(s) 173
PkrI GCNGC 3 cut(s) 275, 844, 947
PleI GAGTC 6 cut(s) 88, 406, 511, 786, 861, 1098
PpsI GAGTC 6 cut(s) 88, 406, 511, 786, 861, 1098
PshAI GACNNNNGTC 1 cut(s) 74
PsiI TTATAA 1 cut(s) 1046
Psp6I CCWGG 1 cut(s) 172
PspGI CCWGG 1 cut(s) 172
PspN4I GGNNCC 2 cut(s) 924, 1002
PspPI GGNCC 3 cut(s) 246, 331, 743
PstNI CAGNNNCTG 1 cut(s) 173
PsuI RGATCY 2 cut(s) 88, 343
PvuII CAGCTG 1 cut(s) 945
RsaI GTAC 4 cut(s) 13, 212, 312, 374
RsaNI GTAC 4 cut(s) 12, 211, 311, 373
RseI CAYNNNNRTG 2 cut(s) 264, 710
SaqAI TTAA 1 cut(s) 762
SatI GCNGC 3 cut(s) 274, 843, 946
Sau3AI GATC 4 cut(s) 88, 343, 484, 525
Sau96I GGNCC 3 cut(s) 246, 331, 743
SchI GAGTC 6 cut(s) 89, 406, 511, 787, 862, 1098
ScrFI CCNGG 3 cut(s) 174, 306, 847
SfaNI GCATC 2 cut(s) 130, 813
SfcI CTRYAG 1 cut(s) 288
SmiMI CAYNNNNRTG 2 cut(s) 264, 710
SmlI CTYRAG 2 cut(s) 512, 1106
SmoI CTYRAG 2 cut(s) 512, 1106
Sse9I AATT 8 cut(s) 202, 299, 355, 454, 501, 610, 691, 964
SsiI CCGC 2 cut(s) 229, 271
SspMI CTAG 1 cut(s) 747
StyD4I CCNGG 3 cut(s) 172, 304, 845
StyI CCWWGG 2 cut(s) 996, 1023
TaaI ACNGT 2 cut(s) 240, 875
TaiI ACGT 1 cut(s) 157
TaqI TCGA 5 cut(s) 78, 474, 528, 534, 900
TasI AATT 8 cut(s) 202, 299, 355, 454, 501, 610, 691, 964
TatI WGTACW 2 cut(s) 310, 372
TfiI GAWTC 2 cut(s) 676, 723
Tru1I TTAA 1 cut(s) 762
Tru9I TTAA 1 cut(s) 762
TscAI CASTG 1 cut(s) 688
TseFI GTSAC 2 cut(s) 266, 854
TseI GCWGC 3 cut(s) 273, 842, 945
Tsp45I GTSAC 2 cut(s) 266, 854
TspDTI ATGAA 4 cut(s) 32, 383, 706, 989
TspGWI ACGGA 1 cut(s) 444
TspRI CASTG 1 cut(s) 688
Van91I CCANNNNNTGG 1 cut(s) 173
XapI RAATTY 4 cut(s) 202, 299, 691, 964
XceI RCATGY 1 cut(s) 754
XmnI GAANNNNTTC 2 cut(s) 555, 610
XspI CTAG 1 cut(s) 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.