RchiOBHm_Chr2g0134441

CCR4-associated factor 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000757
Physical Location & Seq
Reverse (-)
51570899 .. 51572698
1800 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50548

Sequence Viewer

Length: 1083 bp
ATGATGATAGTTCAGAGAGGAATTCTGATCTTCCCAATTGGGCCTGCTTCTCACTTCCACGGCGGAGTTCCGGTGGTCTTCCACCGCCGACCTATGCCTCAGATTCCTCTACACCACCATCCTATACCCCTTTATCCTCCTCAATTTCAGAGCCGCGTCCCTGTTTTCCGGGTTGTTGGCGCCCCAATTTGTCGTCAAGGATCTGTGAAGAAAGCCGATCAGTTCAAGGTTCGAAGAGTTTGTCGCTATAATTTCAAAGTCGAGATTGCCGTAATTCAGAGTCTAATCAGGGCCGGCTACCGATTTGCTAGTTTCGACACGGAATTCCCCGGGACAGTGGTTAAATCTGAAATTCCGAAACATCTGATATCTCAAGCTGTTCTGGTTGATGTGTATAAGAAGAAGCACCGAAACGGGTATAAGATGATGAAGACCAATGTGGACCAAACCCATATTATCCACTTGGGTCTTTCACTCTCTTACCCATTTGGGAATTTTCCGGTTTATGATGGTGCCTACTGCTGTTGGGAGTTTAATTTCAATGATTTCGATGTAGACTGTGCTAACCATTTGCGGAATAGTGTCTCGATTGATGTCCTGAAGCGGCAAGGAATAGACTTTTCCGAAAACAAATGCACTGGTATTTGTTCTGCAGACTTTGCTGAGGAGATTAAGAGGTCTGGGCTAGTGGAGTTGCTTCCTTGCTTGACTTGGGCTACTTTCCAGAGTGCATATGATTTTGGGTACTTGATAAAGATGTTCACTGGGAACAAGGAATTGCCAGAGGATATCAAGGAGTTCATGGGGAACGTGAAGACATATTTTGGACCGAATGTGTACGACATCAAGTACATGATGAAGTTTTGTGATGGACTGTTTGGCGGTTTGAATAGCGTGGCAGAAACCCTTGGGGTTGACTGTGTGGCTGGGAGTAGCCACCAGGCTGGTTCTGATAGTCTTTTGACACTGCAAACGTTTGCCAAGATGCTCAAGATGGAGAAGAACAAAGGGAAAAGGCTGTGGGAAGAATGTGGAGGAGTTTTGTTTGGTTCAGGAAACTTGGATTTCTGGCATCATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1 RVT_3

Protein Analysis

360

Amino Acids

40.76

Weight (kDa)

8.81

Isoelectric Point (pI)

43.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 244 - 287 3.4e-06 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 179, 512
AccI GTMKAC 1 cut(s) 555
AccII CGCG 1 cut(s) 156
AciI CCGC 6 cut(s) 63, 85, 154, 574, 604, 882
AclI AACGTT 1 cut(s) 974
AclWI GGATC 1 cut(s) 208
AcsI RAATTY 4 cut(s) 21, 323, 351, 493
AcuI CTGAAG 1 cut(s) 620
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 3 cut(s) 746, 839, 851
AgsI TTSAA 4 cut(s) 226, 256, 541, 889
AjnI CCWGG 1 cut(s) 939
AluBI AGCT 1 cut(s) 377
AluI AGCT 1 cut(s) 377
Alw26I GTCTC 1 cut(s) 589
AlwI GGATC 1 cut(s) 208
Ama87I CYCGRG 1 cut(s) 329
AoxI GGCC 2 cut(s) 41, 291
ApoI RAATTY 4 cut(s) 21, 323, 351, 493
ArsI GACNNNNNNTTYG 2 cut(s) 226, 258
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 4 cut(s) 41, 291, 442, 827
AsuC2I CCSGG 3 cut(s) 170, 330, 331
AsuII TTCGAA 1 cut(s) 232
AvaI CYCGRG 1 cut(s) 329
AvaII GGWCC 2 cut(s) 442, 827
BanI GGYRCC 2 cut(s) 179, 512
BbsI GAAGAC 3 cut(s) 70, 437, 821
BbvCI CCTCAGC 1 cut(s) 663
BccI CCATC 4 cut(s) 126, 503, 863, 988
BceAI ACGGC 2 cut(s) 76, 254
BciT130I CCWGG 1 cut(s) 941
BcnI CCSGG 3 cut(s) 170, 330, 331
BcoDI GTCTC 1 cut(s) 589
BfaI CTAG 2 cut(s) 309, 686
BfmI CTRYAG 1 cut(s) 651
BfoI RGCGCY 1 cut(s) 183
BisI GCNGC 2 cut(s) 154, 605
BlsI GCNGC 2 cut(s) 155, 606
Bme1390I CCNGG 4 cut(s) 170, 330, 331, 941
Bme18I GGWCC 2 cut(s) 442, 827
BmeT110I CYCGRG 1 cut(s) 329
BmgT120I GGNCC 4 cut(s) 41, 291, 442, 827
BmiI GGNNCC 2 cut(s) 181, 514
BmrFI CCNGG 4 cut(s) 170, 330, 331, 941
BmrI ACTGGG 1 cut(s) 774
BmsI GCATC 1 cut(s) 975
BmuI ACTGGG 1 cut(s) 774
BpiI GAAGAC 3 cut(s) 70, 437, 821
Bpu10I CCTNAGC 1 cut(s) 663
Bpu14I TTCGAA 1 cut(s) 232
BpuEI CTTGAG 2 cut(s) 357, 974
BpuMI CCSGG 3 cut(s) 170, 330, 331
BsaHI GRCGYC 1 cut(s) 180
BsaJI CCNNGG 4 cut(s) 58, 328, 329, 907
BsaWI WCCGGW 2 cut(s) 70, 499
BsaXI ACNNNNNCTCC 2 cut(s) 57, 87
Bse118I RCCGGY 1 cut(s) 293
Bse1I ACTGG 2 cut(s) 643, 769
BseBI CCWGG 1 cut(s) 941
BseDI CCNNGG 4 cut(s) 58, 328, 329, 907
BseGI GGATG 1 cut(s) 118
BseMII CTCAG 2 cut(s) 113, 654
BseNI ACTGG 2 cut(s) 643, 769
BseRI GAGGAG 3 cut(s) 129, 680, 1050
BseYI CCCAGC 1 cut(s) 926
Bsh1236I CGCG 1 cut(s) 156
BshFI GGCC 2 cut(s) 43, 293
BshNI GGYRCC 2 cut(s) 179, 512
BsiHKCI CYCGRG 1 cut(s) 329
BsiSI CCGG 5 cut(s) 71, 169, 294, 330, 500
BslFI GGGAC 2 cut(s) 143, 346
BsmAI GTCTC 1 cut(s) 589
BsmFI GGGAC 2 cut(s) 143, 346
BsnI GGCC 2 cut(s) 43, 293
BsoBI CYCGRG 1 cut(s) 329
Bsp119I TTCGAA 1 cut(s) 232
Bsp143I GATC 3 cut(s) 27, 200, 217
BspACI CCGC 6 cut(s) 63, 85, 154, 574, 604, 882
BspANI GGCC 2 cut(s) 43, 293
BspCNI CTCAG 2 cut(s) 112, 655
BspFNI CGCG 1 cut(s) 156
BspLI GGNNCC 2 cut(s) 181, 514
BspMAI CTGCAG 1 cut(s) 655
BspPI GGATC 1 cut(s) 208
BspT104I TTCGAA 1 cut(s) 232
BspT107I GGYRCC 2 cut(s) 179, 512
BsrFI RCCGGY 1 cut(s) 293
BsrI ACTGG 2 cut(s) 643, 769
BssAI RCCGGY 1 cut(s) 293
BssECI CCNNGG 4 cut(s) 58, 328, 329, 907
BssMI GATC 3 cut(s) 27, 200, 217
BssNI GRCGYC 1 cut(s) 180
BssT1I CCWWGG 1 cut(s) 907
Bst2UI CCWGG 1 cut(s) 941
Bst4CI ACNGT 4 cut(s) 337, 560, 876, 920
Bst6I CTCTTC 1 cut(s) 229
BstACI GRCGYC 1 cut(s) 180
BstAPI GCANNNNNTGC 1 cut(s) 659
BstBI TTCGAA 1 cut(s) 232
BstC8I GCNNGC 2 cut(s) 45, 295
BstDEI CTNAG 2 cut(s) 99, 663
BstDSI CCRYGG 1 cut(s) 58
BstF5I GGATG 1 cut(s) 118
BstFNI CGCG 1 cut(s) 156
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 3 cut(s) 30, 203, 220
BstMAI GTCTC 1 cut(s) 589
BstMBI GATC 3 cut(s) 27, 200, 217
BstMWI GCNNNNNNNGC 1 cut(s) 659
BstNI CCWGG 1 cut(s) 941
BstSCI CCNGG 4 cut(s) 168, 328, 329, 939
BstSFI CTRYAG 1 cut(s) 651
BstUI CGCG 1 cut(s) 156
BstV2I GAAGAC 3 cut(s) 70, 437, 821
BstX2I RGATCY 1 cut(s) 200
BstXI CCANNNNNNTGG 1 cut(s) 944
BstYI RGATCY 1 cut(s) 200
BsuRI GGCC 2 cut(s) 43, 293
BtgI CCRYGG 1 cut(s) 58
BtsCI GGATG 1 cut(s) 118
BtsI GCAGTG 1 cut(s) 965
BtsIMutI CAGTG 4 cut(s) 342, 636, 762, 965
Cac8I GCNNGC 2 cut(s) 45, 295
CfoI GCGC 1 cut(s) 182
Cfr10I RCCGGY 1 cut(s) 293
Cfr13I GGNCC 4 cut(s) 41, 291, 442, 827
Cfr9I CCCGGG 1 cut(s) 329
CseI GACGC 1 cut(s) 145
Csp6I GTAC 3 cut(s) 745, 838, 850
CviAII CATG 2 cut(s) 802, 853
CviQI GTAC 3 cut(s) 745, 838, 850
DdeI CTNAG 2 cut(s) 99, 663
DinI GGCGCC 1 cut(s) 181
DpnI GATC 3 cut(s) 29, 202, 219
DpnII GATC 3 cut(s) 27, 200, 217
Eam1104I CTCTTC 1 cut(s) 229
EarI CTCTTC 1 cut(s) 229
EciI GGCGGA 1 cut(s) 78
Eco130I CCWWGG 1 cut(s) 907
Eco32I GATATC 2 cut(s) 369, 790
Eco47I GGWCC 2 cut(s) 442, 827
Eco57I CTGAAG 1 cut(s) 620
Eco88I CYCGRG 1 cut(s) 329
EcoRI GAATTC 2 cut(s) 21, 323
EcoRII CCWGG 1 cut(s) 939
EcoRV GATATC 2 cut(s) 369, 790
EcoT14I CCWWGG 1 cut(s) 907
EgeI GGCGCC 1 cut(s) 181
EheI GGCGCC 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 907
FaeI CATG 2 cut(s) 805, 856
FaqI GGGAC 2 cut(s) 143, 346
FatI CATG 2 cut(s) 801, 852
FauNDI CATATG 1 cut(s) 733
FblI GTMKAC 1 cut(s) 555
Fnu4HI GCNGC 2 cut(s) 154, 605
FokI GGATG 1 cut(s) 105
Fsp4HI GCNGC 2 cut(s) 154, 605
FspBI CTAG 2 cut(s) 309, 686
GlaI GCGC 1 cut(s) 181
GluI GCNGC 2 cut(s) 154, 605
GsaI CCCAGC 1 cut(s) 930
HaeII RGCGCY 1 cut(s) 183
HaeIII GGCC 2 cut(s) 43, 293
HapII CCGG 5 cut(s) 71, 169, 294, 330, 500
HgaI GACGC 1 cut(s) 145
HhaI GCGC 1 cut(s) 182
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 2 cut(s) 805, 856
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HincII GTYRAC 1 cut(s) 916
HindII GTYRAC 1 cut(s) 916
HinfI GANTC 2 cut(s) 103, 280
HpaII CCGG 5 cut(s) 71, 169, 294, 330, 500
Hpy166II GTNNAC 5 cut(s) 442, 556, 762, 838, 916
Hpy188III TCNNGA 6 cut(s) 262, 586, 598, 724, 991, 1053
Hpy8I GTNNAC 5 cut(s) 442, 556, 762, 838, 916
HpyCH4III ACNGT 4 cut(s) 337, 560, 876, 920
HpyCH4IV ACGT 2 cut(s) 810, 974
HpyCH4V TGCA 4 cut(s) 636, 653, 731, 970
HpyF10VI GCNNNNNNNGC 1 cut(s) 659
HpyF3I CTNAG 2 cut(s) 99, 663
HpySE526I ACGT 2 cut(s) 810, 974
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 2 cut(s) 805, 856
HspAI GCGC 1 cut(s) 180
KasI GGCGCC 1 cut(s) 179
KroI GCCGGC 1 cut(s) 293
KroNI GCCGGC 1 cut(s) 295
Kzo9I GATC 3 cut(s) 27, 200, 217
LweI GCATC 1 cut(s) 975
MaeI CTAG 2 cut(s) 309, 686
MaeII ACGT 2 cut(s) 810, 974
MalI GATC 3 cut(s) 29, 202, 219
MboI GATC 3 cut(s) 27, 200, 217
MboII GAAGA 9 cut(s) 22, 70, 220, 246, 412, 442, 826, 1012, 1037
MfeI CAATTG 1 cut(s) 36
MflI RGATCY 1 cut(s) 200
Mly113I GGCGCC 1 cut(s) 180
MlyI GAGTC 1 cut(s) 289
MnlI CCTC 9 cut(s) 11, 108, 117, 147, 150, 658, 669, 778, 1028
MroNI GCCGGC 1 cut(s) 293
MseI TTAA 3 cut(s) 342, 534, 672
MspI CCGG 5 cut(s) 71, 169, 294, 330, 500
MspR9I CCNGG 4 cut(s) 170, 330, 331, 941
MunI CAATTG 1 cut(s) 36
MvaI CCWGG 1 cut(s) 941
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 1 cut(s) 659
NaeI GCCGGC 1 cut(s) 295
NarI GGCGCC 1 cut(s) 180
NciI CCSGG 3 cut(s) 170, 330, 331
NdeI CATATG 1 cut(s) 733
NdeII GATC 3 cut(s) 27, 200, 217
NgoMIV GCCGGC 1 cut(s) 293
NlaIII CATG 2 cut(s) 805, 856
NlaIV GGNNCC 2 cut(s) 181, 514
NspV TTCGAA 1 cut(s) 232
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PdiI GCCGGC 1 cut(s) 295
PfeI GAWTC 1 cut(s) 103
PkrI GCNGC 2 cut(s) 155, 606
PleI GAGTC 1 cut(s) 288
PluTI GGCGCC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 288
Psp1406I AACGTT 1 cut(s) 974
Psp6I CCWGG 1 cut(s) 939
PspFI CCCAGC 1 cut(s) 926
PspGI CCWGG 1 cut(s) 939
PspN4I GGNNCC 2 cut(s) 181, 514
PspPI GGNCC 4 cut(s) 41, 291, 442, 827
PstI CTGCAG 1 cut(s) 655
PsuI RGATCY 1 cut(s) 200
RsaI GTAC 3 cut(s) 746, 839, 851
RsaNI GTAC 3 cut(s) 745, 838, 850
SaqAI TTAA 3 cut(s) 342, 534, 672
SatI GCNGC 2 cut(s) 154, 605
Sau3AI GATC 3 cut(s) 27, 200, 217
Sau96I GGNCC 4 cut(s) 41, 291, 442, 827
SchI GAGTC 1 cut(s) 289
ScrFI CCNGG 4 cut(s) 170, 330, 331, 941
SetI ASST 6 cut(s) 94, 231, 379, 680, 813, 977
SfaNI GCATC 1 cut(s) 975
SfcI CTRYAG 1 cut(s) 651
SfoI GGCGCC 1 cut(s) 181
SfuI TTCGAA 1 cut(s) 232
SinI GGWCC 2 cut(s) 442, 827
SmaI CCCGGG 1 cut(s) 331
SmlI CTYRAG 2 cut(s) 372, 989
SmoI CTYRAG 2 cut(s) 372, 989
SsiI CCGC 6 cut(s) 63, 85, 154, 574, 604, 882
SspDI GGCGCC 1 cut(s) 179
SspMI CTAG 2 cut(s) 309, 686
StyD4I CCNGG 4 cut(s) 168, 328, 329, 939
StyI CCWWGG 1 cut(s) 907
TaaI ACNGT 4 cut(s) 337, 560, 876, 920
TaiI ACGT 2 cut(s) 813, 977
TaqI TCGA 5 cut(s) 232, 261, 315, 549, 587
TaqII GACCGA 1 cut(s) 844
TatI WGTACW 1 cut(s) 849
TauI GCSGC 2 cut(s) 156, 607
TfiI GAWTC 1 cut(s) 103
Tru1I TTAA 3 cut(s) 342, 534, 672
Tru9I TTAA 3 cut(s) 342, 534, 672
TscAI CASTG 4 cut(s) 342, 643, 769, 972
TspDTI ATGAA 3 cut(s) 443, 790, 872
TspGWI ACGGA 1 cut(s) 335
TspMI CCCGGG 1 cut(s) 329
TspRI CASTG 4 cut(s) 342, 643, 769, 972
VpaK11BI GGWCC 2 cut(s) 442, 827
XapI RAATTY 4 cut(s) 21, 323, 351, 493
XmaI CCCGGG 1 cut(s) 329
XmiI GTMKAC 1 cut(s) 555
XspI CTAG 2 cut(s) 309, 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.