Rw2G028830

CCR4-associated factor 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000757
Physical Location & Seq
Forward (+)
46305671 .. 46306534
864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G028830.1

Sequence Viewer

Length: 864 bp
ATGCAAGCTGTCGAAGGTACACCATGTCTGCAGTTTCCCTCACCTCCTGTGCCGGTCATAAATCCTTACTTGGGTACAATGGAGTACTGCTGCCCAATTATTCCCCTTCCCATGCATGATCCCGACCCTCTTCCTATACCTGGCGTTATATATCCGGTTTGGAAGAGCAATTTAGTGCCTGCGTTTTCGCAGATTTGGAAACTAATTAGCCAAGGTGGCCGCTGGTTCGCTTCTATAGACACAGAATTCCCTGGCACAATTTATGATTACGACAAAGGCCGTCTCCATGACCCTCTGTATAAGTATATGATGATGAAGAAGAACGTGAACGACACCAACATAATCCAGTTGGGTCTCACACTCGCAGACCTTAACCAAAACATCTATACCTGGGAGTTCAATTTCAGCGACTTTGATATTGACCGTGAAGATCACAAGCATATCAAGCGCTCAATTGAGTTACTTAAGCGTCACGGAATCGATTTCTGGAGAAACAAGGAGGATGGTATACCTTCGCATGAGTTCGCGAGAATGTGTCGGGAAGTTGGGCTATTAGAAAACAGATTGGTAGAGTTGACATGGGTGGGTTTCCAGATCTCATCTGATTTGGGCTACTTCACCAAGATCCTCACTGGGCAAAAGTTGCCAGACGATTTTGAGGAGTACAAAAAGGCTGAAAGGATCTACTTTGGGGATAGGGTTTGTGATGTCAAGGACATGATGAACTCGTTTCCGACACTGAAAGGCGGTTTGACCAAAGTGGCGGAGAAACTGGGGATTGCTCGTGTGGCCGGCAAGAGTCACCAGGCCGGCTCTGATAGTCTGTTGACATATCTAAGCTTCATCAAGCTGAGCTCAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1

Protein Analysis

287

Amino Acids

32.92

Weight (kDa)

5.99

Isoelectric Point (pI)

38.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 53 - 171 4.1e-10 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 508
AccII CGCG 1 cut(s) 527
AciI CCGC 3 cut(s) 220, 747, 764
AclWI GGATC 3 cut(s) 113, 619, 689
AcoI YGGCCR 2 cut(s) 217, 789
AcsI RAATTY 1 cut(s) 245
AfaI GTAC 4 cut(s) 19, 76, 86, 665
AfeI AGCGCT 1 cut(s) 449
AfiI CCNNNNNNNGG 2 cut(s) 71, 140
AflII CTTAAG 1 cut(s) 464
AgsI TTSAA 1 cut(s) 400
AjnI CCWGG 4 cut(s) 139, 250, 389, 804
AluBI AGCT 4 cut(s) 8, 840, 850, 855
AluI AGCT 4 cut(s) 8, 840, 850, 855
Alw21I GWGCWC 1 cut(s) 857
Alw26I GTCTC 2 cut(s) 287, 359
AlwI GGATC 3 cut(s) 113, 619, 689
Aor51HI AGCGCT 1 cut(s) 449
AoxI GGCC 4 cut(s) 217, 277, 789, 807
ApeKI GCWGC 1 cut(s) 90
ApoI RAATTY 1 cut(s) 245
AspLEI GCGC 1 cut(s) 450
AsuHPI GGTGA 3 cut(s) 33, 610, 794
BanII GRGCYC 1 cut(s) 857
BauI CACGAG 1 cut(s) 783
Bbv12I GWGCWC 1 cut(s) 857
BbvI GCAGC 1 cut(s) 77
BccI CCATC 1 cut(s) 497
BceAI ACGGC 1 cut(s) 264
BciT130I CCWGG 4 cut(s) 141, 252, 391, 806
BcoDI GTCTC 2 cut(s) 287, 359
BfmI CTRYAG 2 cut(s) 29, 234
BfoI RGCGCY 1 cut(s) 451
BfrI CTTAAG 1 cut(s) 464
BglI GCCNNNNNGGC 1 cut(s) 216
BglII AGATCT 1 cut(s) 594
BisI GCNGC 2 cut(s) 91, 220
BlpI GCTNAGC 1 cut(s) 851
BlsI GCNGC 2 cut(s) 92, 221
BmcAI AGTACT 1 cut(s) 86
Bme1390I CCNGG 4 cut(s) 141, 252, 391, 806
BmrFI CCNGG 4 cut(s) 141, 252, 391, 806
BmrI ACTGGG 2 cut(s) 642, 782
BmuI ACTGGG 2 cut(s) 642, 782
BpmI CTGGAG 1 cut(s) 508
Bpu1102I GCTNAGC 1 cut(s) 851
Bsa29I ATCGAT 1 cut(s) 480
BsaI GGTCTC 1 cut(s) 359
BsaJI CCNNGG 3 cut(s) 211, 250, 390
BsaWI WCCGGW 1 cut(s) 154
Bsc4I CCNNNNNNNGG 2 cut(s) 71, 140
Bse118I RCCGGY 3 cut(s) 52, 791, 809
Bse1I ACTGG 3 cut(s) 346, 637, 777
BseBI CCWGG 4 cut(s) 141, 252, 391, 806
BseCI ATCGAT 1 cut(s) 480
BseDI CCNNGG 3 cut(s) 211, 250, 390
BseGI GGATG 1 cut(s) 508
BseLI CCNNNNNNNGG 2 cut(s) 71, 140
BseMII CTCAG 1 cut(s) 842
BseNI ACTGG 3 cut(s) 346, 637, 777
BseRI GAGGAG 1 cut(s) 674
BseXI GCAGC 1 cut(s) 77
Bsh1236I CGCG 1 cut(s) 527
BshFI GGCC 4 cut(s) 219, 279, 791, 809
BshVI ATCGAT 1 cut(s) 480
BsiHKAI GWGCWC 1 cut(s) 857
BsiSI CCGG 4 cut(s) 53, 155, 792, 810
BslI CCNNNNNNNGG 2 cut(s) 71, 140
BsmAI GTCTC 2 cut(s) 287, 359
BsmBI CGTCTC 1 cut(s) 287
BsnI GGCC 4 cut(s) 219, 279, 791, 809
Bso31I GGTCTC 1 cut(s) 359
Bsp1286I GDGCHC 1 cut(s) 857
Bsp143I GATC 5 cut(s) 118, 430, 594, 624, 681
Bsp1720I GCTNAGC 1 cut(s) 851
Bsp68I TCGCGA 1 cut(s) 527
BspACI CCGC 3 cut(s) 220, 747, 764
BspANI GGCC 4 cut(s) 219, 279, 791, 809
BspCNI CTCAG 1 cut(s) 843
BspDI ATCGAT 1 cut(s) 480
BspFNI CGCG 1 cut(s) 527
BspMAI CTGCAG 1 cut(s) 33
BspPI GGATC 3 cut(s) 113, 619, 689
BspQI GCTCTTC 1 cut(s) 158
BspTI CTTAAG 1 cut(s) 464
BspTNI GGTCTC 1 cut(s) 359
BsrFI RCCGGY 3 cut(s) 52, 791, 809
BsrI ACTGG 3 cut(s) 346, 637, 777
BssAI RCCGGY 3 cut(s) 52, 791, 809
BssECI CCNNGG 3 cut(s) 211, 250, 390
BssMI GATC 5 cut(s) 118, 430, 594, 624, 681
BssNAI GTATAC 1 cut(s) 509
BssSI CACGAG 1 cut(s) 783
BssT1I CCWWGG 1 cut(s) 211
Bst1107I GTATAC 1 cut(s) 509
Bst2BI CACGAG 1 cut(s) 783
Bst2UI CCWGG 4 cut(s) 141, 252, 391, 806
Bst4CI ACNGT 1 cut(s) 425
Bst6I CTCTTC 2 cut(s) 135, 158
BstAFI CTTAAG 1 cut(s) 464
BstAPI GCANNNNNTGC 1 cut(s) 643
BstC8I GCNNGC 4 cut(s) 6, 180, 793, 811
BstDEI CTNAG 2 cut(s) 836, 851
BstF5I GGATG 1 cut(s) 508
BstFNI CGCG 1 cut(s) 527
BstH2I RGCGCY 1 cut(s) 451
BstHHI GCGC 1 cut(s) 450
BstKTI GATC 5 cut(s) 121, 433, 597, 627, 684
BstMAI GTCTC 2 cut(s) 287, 359
BstMBI GATC 5 cut(s) 118, 430, 594, 624, 681
BstMWI GCNNNNNNNGC 4 cut(s) 216, 445, 643, 788
BstNI CCWGG 4 cut(s) 141, 252, 391, 806
BstSCI CCNGG 4 cut(s) 139, 250, 389, 804
BstSFI CTRYAG 2 cut(s) 29, 234
BstUI CGCG 1 cut(s) 527
BstV1I GCAGC 1 cut(s) 77
BstX2I RGATCY 3 cut(s) 594, 624, 681
BstYI RGATCY 3 cut(s) 594, 624, 681
BstZ17I GTATAC 1 cut(s) 509
Bsu15I ATCGAT 1 cut(s) 480
BsuRI GGCC 4 cut(s) 219, 279, 791, 809
BsuTUI ATCGAT 1 cut(s) 480
BtsCI GGATG 1 cut(s) 508
BtsIMutI CAGTG 2 cut(s) 630, 737
BtuMI TCGCGA 1 cut(s) 527
Cac8I GCNNGC 4 cut(s) 6, 180, 793, 811
CfoI GCGC 1 cut(s) 450
Cfr10I RCCGGY 3 cut(s) 52, 791, 809
ClaI ATCGAT 1 cut(s) 480
CseI GACGC 1 cut(s) 458
Csp6I GTAC 4 cut(s) 18, 75, 85, 664
CviAII CATG 7 cut(s) 24, 112, 116, 287, 518, 579, 718
CviQI GTAC 4 cut(s) 18, 75, 85, 664
DdeI CTNAG 2 cut(s) 836, 851
DpnI GATC 5 cut(s) 120, 432, 596, 626, 683
DpnII GATC 5 cut(s) 118, 430, 594, 624, 681
EaeI YGGCCR 2 cut(s) 217, 789
Eam1104I CTCTTC 2 cut(s) 135, 158
EarI CTCTTC 2 cut(s) 135, 158
EciI GGCGGA 1 cut(s) 779
Ecl136II GAGCTC 1 cut(s) 855
Eco130I CCWWGG 1 cut(s) 211
Eco24I GRGCYC 1 cut(s) 857
Eco31I GGTCTC 1 cut(s) 359
Eco47III AGCGCT 1 cut(s) 449
Eco53kI GAGCTC 1 cut(s) 855
EcoICRI GAGCTC 1 cut(s) 855
EcoRI GAATTC 1 cut(s) 245
EcoRII CCWGG 4 cut(s) 139, 250, 389, 804
EcoT14I CCWWGG 1 cut(s) 211
EcoT22I ATGCAT 1 cut(s) 117
EcoT38I GRGCYC 1 cut(s) 857
ErhI CCWWGG 1 cut(s) 211
Esp3I CGTCTC 1 cut(s) 287
FaeI CATG 7 cut(s) 27, 115, 119, 290, 521, 582, 721
FatI CATG 7 cut(s) 23, 111, 115, 286, 517, 578, 717
FblI GTMKAC 1 cut(s) 508
Fnu4HI GCNGC 2 cut(s) 91, 220
FokI GGATG 1 cut(s) 515
FriOI GRGCYC 1 cut(s) 857
Fsp4HI GCNGC 2 cut(s) 91, 220
GlaI GCGC 1 cut(s) 449
GluI GCNGC 2 cut(s) 91, 220
GsuI CTGGAG 1 cut(s) 508
HaeII RGCGCY 1 cut(s) 451
HaeIII GGCC 4 cut(s) 219, 279, 791, 809
HapII CCGG 4 cut(s) 53, 155, 792, 810
HgaI GACGC 1 cut(s) 458
HhaI GCGC 1 cut(s) 450
Hin1II CATG 7 cut(s) 27, 115, 119, 290, 521, 582, 721
Hin6I GCGC 1 cut(s) 448
HinP1I GCGC 1 cut(s) 448
HincII GTYRAC 2 cut(s) 576, 828
HindII GTYRAC 2 cut(s) 576, 828
HindIII AAGCTT 1 cut(s) 838
HinfI GANTC 2 cut(s) 477, 799
HpaII CCGG 4 cut(s) 53, 155, 792, 810
HphI GGTGA 3 cut(s) 33, 610, 794
Hpy166II GTNNAC 5 cut(s) 20, 328, 509, 576, 828
Hpy188I TCNGA 3 cut(s) 604, 735, 817
Hpy188III TCNNGA 5 cut(s) 122, 487, 526, 539, 592
Hpy8I GTNNAC 5 cut(s) 20, 328, 509, 576, 828
HpyAV CCTTC 3 cut(s) 8, 116, 522
HpyCH4III ACNGT 1 cut(s) 425
HpyCH4IV ACGT 1 cut(s) 324
HpyCH4V TGCA 3 cut(s) 4, 31, 115
HpyF10VI GCNNNNNNNGC 4 cut(s) 216, 445, 643, 788
HpyF3I CTNAG 2 cut(s) 836, 851
HpySE526I ACGT 1 cut(s) 324
Hsp92II CATG 7 cut(s) 27, 115, 119, 290, 521, 582, 721
HspAI GCGC 1 cut(s) 448
KroI GCCGGC 2 cut(s) 791, 809
KroNI GCCGGC 2 cut(s) 793, 811
Kzo9I GATC 5 cut(s) 118, 430, 594, 624, 681
LguI GCTCTTC 1 cut(s) 158
Lsp1109I GCAGC 1 cut(s) 77
MaeII ACGT 1 cut(s) 324
MaeIII GTNAC 3 cut(s) 459, 470, 800
MalI GATC 5 cut(s) 120, 432, 596, 626, 683
MboI GATC 5 cut(s) 118, 430, 594, 624, 681
MboII GAAGA 5 cut(s) 122, 175, 328, 331, 440
MfeI CAATTG 1 cut(s) 453
MflI RGATCY 3 cut(s) 594, 624, 681
MhlI GDGCHC 1 cut(s) 857
MluCI AATT 7 cut(s) 96, 169, 204, 245, 258, 400, 453
MlyI GAGTC 1 cut(s) 808
MmeI TCCRAC 1 cut(s) 758
MnlI CCTC 7 cut(s) 49, 54, 138, 303, 493, 638, 652
Mph1103I ATGCAT 1 cut(s) 117
MroNI GCCGGC 2 cut(s) 791, 809
MseI TTAA 2 cut(s) 372, 465
MspA1I CMGCKG 1 cut(s) 222
MspCI CTTAAG 1 cut(s) 464
MspI CCGG 4 cut(s) 53, 155, 792, 810
MspR9I CCNGG 4 cut(s) 141, 252, 391, 806
MunI CAATTG 1 cut(s) 453
MvaI CCWGG 4 cut(s) 141, 252, 391, 806
MvnI CGCG 1 cut(s) 527
MwoI GCNNNNNNNGC 4 cut(s) 216, 445, 643, 788
NaeI GCCGGC 2 cut(s) 793, 811
NdeII GATC 5 cut(s) 118, 430, 594, 624, 681
NgoMIV GCCGGC 2 cut(s) 791, 809
NlaIII CATG 7 cut(s) 27, 115, 119, 290, 521, 582, 721
NmuCI GTSAC 2 cut(s) 470, 800
NruI TCGCGA 1 cut(s) 527
NsiI ATGCAT 1 cut(s) 117
PciSI GCTCTTC 1 cut(s) 158
PdiI GCCGGC 2 cut(s) 793, 811
PfeI GAWTC 1 cut(s) 477
PkrI GCNGC 2 cut(s) 92, 221
PleI GAGTC 1 cut(s) 807
PpsI GAGTC 1 cut(s) 807
Psp124BI GAGCTC 1 cut(s) 857
Psp6I CCWGG 4 cut(s) 139, 250, 389, 804
PspGI CCWGG 4 cut(s) 139, 250, 389, 804
PstI CTGCAG 1 cut(s) 33
PsuI RGATCY 3 cut(s) 594, 624, 681
RruI TCGCGA 1 cut(s) 527
RsaI GTAC 4 cut(s) 19, 76, 86, 665
RsaNI GTAC 4 cut(s) 18, 75, 85, 664
SacI GAGCTC 1 cut(s) 857
SapI GCTCTTC 1 cut(s) 158
SaqAI TTAA 2 cut(s) 372, 465
SatI GCNGC 2 cut(s) 91, 220
Sau3AI GATC 5 cut(s) 118, 430, 594, 624, 681
ScaI AGTACT 1 cut(s) 86
SchI GAGTC 1 cut(s) 808
ScrFI CCNGG 4 cut(s) 141, 252, 391, 806
SduI GDGCHC 1 cut(s) 857
SfcI CTRYAG 2 cut(s) 29, 234
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
Sse9I AATT 7 cut(s) 96, 169, 204, 245, 258, 400, 453
SsiI CCGC 3 cut(s) 220, 747, 764
SstI GAGCTC 1 cut(s) 857
StyD4I CCNGG 4 cut(s) 139, 250, 389, 804
StyI CCWWGG 1 cut(s) 211
TaaI ACNGT 1 cut(s) 425
TaiI ACGT 1 cut(s) 327
TaqI TCGA 2 cut(s) 12, 480
TasI AATT 7 cut(s) 96, 169, 204, 245, 258, 400, 453
TatI WGTACW 2 cut(s) 84, 663
TauI GCSGC 1 cut(s) 222
TfiI GAWTC 1 cut(s) 477
Tru1I TTAA 2 cut(s) 372, 465
Tru9I TTAA 2 cut(s) 372, 465
TscAI CASTG 2 cut(s) 637, 744
TseFI GTSAC 2 cut(s) 470, 800
TseI GCWGC 1 cut(s) 90
Tsp45I GTSAC 2 cut(s) 470, 800
TspDTI ATGAA 3 cut(s) 329, 737, 832
TspGWI ACGGA 1 cut(s) 489
TspRI CASTG 2 cut(s) 637, 744
Vha464I CTTAAG 1 cut(s) 464
XapI RAATTY 1 cut(s) 245
XmiI GTMKAC 1 cut(s) 508
ZrmI AGTACT 1 cut(s) 86
Zsp2I ATGCAT 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.