RLG00000019226

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000757
Physical Location & Seq
Forward (+)
48627436 .. 48628539
1104 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019226

Sequence Viewer

Length: 1104 bp
ATGATGCACGCAGCTACTGTAGGTATACCATATCCGCCGATTTATCATCATTATCCTTACCTTCTTCAGCCAATTGCCGCTTCGGACTGCTTATATCCTATGCAGAACCATCTGGTGCCCAATCCCCACCCTATTATACTTCCAATCCCTGGCATAATCAATCGGGTTTGGCACAAGAATTTGGAGTCTGAGTTTAAGAAAATCCGGAACTTACTCTTTTGGCCCGGTGAGCCCCATACGCATGACCGCTGCTTTCTTGCTTCTATAGACACTGAATTCCCCGGTCAACTGTCTGAACCTGACAAAGGCCGTCTCCGAGAGCCGCAGTACAATTATGAGATGATGAAGAACAATGTGAACGCCACCAACATAATCCAATTGGGTTTGACTCTCTCGGACAGATTTGGAAACTTCTACGTATGGGAGTTCAATTTCAGTGACTTTGATATCGAAAGTGATAATCAAAACAAGGTCTCAATTGAGCTTCTCAAGACTCATGGGACCGATTTCCAGAGAAACAAGAAGGATGGTATTCCTTCCTCTAAGTTCGCAGAGTTGTGTCGGGGCTCTGGGCTGGTAGGAAACAGTTCAGAGGTACATTTGACATGGGTGGGTTTCCAGAGTTCTTATGATTTTGGCTTCTTCACCAAGATTCTCAGTGGGCAAAAGTTGCCAGACAACATGCATGGGTTCATGAGAAATGTGAAGAACTACTTTGGGCCTAGCGTGTTTGACATGAAACACATGATCACGAGGTTCTGTTCCGGAATTTATGGGGGTTTGGAGAAGGTGGCAGAGACGCTCGGGGTTGCTCGGGCCGCCGGGAAGAGTCACATGGCTGGTTCTGACAGTCTTTTGACATTGCAAACCTTTCTCAAGCTTATAAATGTGTGTTTGAAGCCAGCAGATTCTAACAGCAGCAGCATTGAGACATTGCAGAAGTTTACAATGAATATGAGTTTTGTGAATTCCCGCGCTCCTTGTGATATCGGTTTGGCATTGCAGGTCTTTAAGATGCTTATAAGACTTGAGGAAGAAAGAGAAAAACAGAACTACTTTAAGTTGTATAGTTGTCAAGGGATTTTGTATGGTTTAGCTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1

Protein Analysis

368

Amino Acids

41.7

Weight (kDa)

7.66

Isoelectric Point (pI)

40.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 84 - 179 4e-09 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 884, 1022
Acc36I ACCTGC 1 cut(s) 994
AccB1I GGYRCC 1 cut(s) 115
AccB7I CCANNNNNTGG 1 cut(s) 149
AccI GTMKAC 1 cut(s) 25
AccII CGCG 1 cut(s) 975
AccIII TCCGGA 2 cut(s) 204, 764
AciI CCGC 6 cut(s) 35, 78, 247, 323, 819, 973
AcsI RAATTY 4 cut(s) 178, 275, 768, 967
AcuI CTGAAG 1 cut(s) 50
AfaI GTAC 2 cut(s) 329, 597
AfiI CCNNNNNNNGG 2 cut(s) 149, 305
AgsI TTSAA 2 cut(s) 430, 898
AjnI CCWGG 1 cut(s) 148
AjuI GAANNNNNNNTTGG 2 cut(s) 64, 96
AluBI AGCT 4 cut(s) 14, 484, 880, 1097
AluI AGCT 4 cut(s) 14, 484, 880, 1097
Alw26I GTCTC 4 cut(s) 317, 478, 791, 923
AlwNI CAGNNNCTG 1 cut(s) 17
Ama87I CYCGRG 2 cut(s) 803, 813
Aor13HI TCCGGA 2 cut(s) 204, 764
AoxI GGCC 4 cut(s) 221, 307, 719, 816
ApeKI GCWGC 4 cut(s) 11, 249, 918, 921
ApoI RAATTY 4 cut(s) 178, 275, 768, 967
Asp700I GAANNNNTTC 1 cut(s) 586
AspLEI GCGC 1 cut(s) 977
AspS9I GGNCC 4 cut(s) 222, 501, 719, 816
AsuC2I CCSGG 3 cut(s) 225, 282, 823
AsuHPI GGTGA 2 cut(s) 239, 637
AvaI CYCGRG 2 cut(s) 803, 813
AvaII GGWCC 1 cut(s) 501
BaeGI GKGCMC 1 cut(s) 120
BanI GGYRCC 1 cut(s) 115
BanII GRGCYC 2 cut(s) 234, 569
BauI CACGAG 1 cut(s) 751
BbvI GCAGC 4 cut(s) 23, 236, 930, 933
BccI CCATC 2 cut(s) 117, 521
BceAI ACGGC 1 cut(s) 294
BciT130I CCWGG 1 cut(s) 150
BclI TGATCA 1 cut(s) 747
BcnI CCSGG 3 cut(s) 225, 282, 823
BcoDI GTCTC 4 cut(s) 317, 478, 791, 923
BfaI CTAG 2 cut(s) 723, 1098
BfmI CTRYAG 2 cut(s) 18, 264
BfuAI ACCTGC 1 cut(s) 994
BisI GCNGC 7 cut(s) 12, 78, 250, 323, 819, 919, 922
BlsI GCNGC 7 cut(s) 13, 79, 251, 324, 820, 920, 923
Bme1390I CCNGG 4 cut(s) 150, 225, 282, 823
Bme18I GGWCC 1 cut(s) 501
BmeT110I CYCGRG 2 cut(s) 803, 813
BmgT120I GGNCC 4 cut(s) 222, 501, 719, 816
BmiI GGNNCC 2 cut(s) 117, 502
BmrFI CCNGG 4 cut(s) 150, 225, 282, 823
BmsI GCATC 1 cut(s) 1005
BpuEI CTTGAG 3 cut(s) 473, 860, 1049
BpuMI CCSGG 3 cut(s) 225, 282, 823
BsaAI YACGTR 1 cut(s) 418
BsaI GGTCTC 1 cut(s) 478
BsaJI CCNNGG 2 cut(s) 148, 280
BsaWI WCCGGW 2 cut(s) 204, 764
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
Bsc4I CCNNNNNNNGG 2 cut(s) 149, 305
Bse3DI GCAATG 3 cut(s) 860, 932, 998
BseAI TCCGGA 2 cut(s) 204, 764
BseBI CCWGG 1 cut(s) 150
BseDI CCNNGG 2 cut(s) 148, 280
BseGI GGATG 1 cut(s) 532
BseLI CCNNNNNNNGG 2 cut(s) 149, 305
BseMI GCAATG 3 cut(s) 860, 932, 998
BseMII CTCAG 2 cut(s) 180, 670
BseSI GKGCMC 1 cut(s) 120
BseXI GCAGC 4 cut(s) 23, 236, 930, 933
Bsh1236I CGCG 1 cut(s) 975
BshFI GGCC 4 cut(s) 223, 309, 721, 818
BshNI GGYRCC 1 cut(s) 115
BsiHKCI CYCGRG 2 cut(s) 803, 813
BsiSI CCGG 5 cut(s) 205, 225, 282, 765, 822
BslFI GGGAC 1 cut(s) 514
BslI CCNNNNNNNGG 2 cut(s) 149, 305
BsmAI GTCTC 4 cut(s) 317, 478, 791, 923
BsmBI CGTCTC 2 cut(s) 317, 791
BsmFI GGGAC 1 cut(s) 514
BsnI GGCC 4 cut(s) 223, 309, 721, 818
Bso31I GGTCTC 1 cut(s) 478
BsoBI CYCGRG 2 cut(s) 803, 813
Bsp1286I GDGCHC 3 cut(s) 120, 234, 569
Bsp13I TCCGGA 2 cut(s) 204, 764
Bsp143I GATC 1 cut(s) 747
BspACI CCGC 6 cut(s) 35, 78, 247, 323, 819, 973
BspANI GGCC 4 cut(s) 223, 309, 721, 818
BspCNI CTCAG 2 cut(s) 181, 669
BspEI TCCGGA 2 cut(s) 204, 764
BspFNI CGCG 1 cut(s) 975
BspHI TCATGA 1 cut(s) 693
BspLI GGNNCC 2 cut(s) 117, 502
BspMI ACCTGC 1 cut(s) 994
BspT107I GGYRCC 1 cut(s) 115
BspTNI GGTCTC 1 cut(s) 478
BsrDI GCAATG 3 cut(s) 860, 932, 998
BssECI CCNNGG 2 cut(s) 148, 280
BssMI GATC 1 cut(s) 747
BssNAI GTATAC 1 cut(s) 26
BssSI CACGAG 1 cut(s) 751
Bst1107I GTATAC 1 cut(s) 26
Bst2BI CACGAG 1 cut(s) 751
Bst2UI CCWGG 1 cut(s) 150
Bst4CI ACNGT 4 cut(s) 19, 291, 587, 851
Bst6I CTCTTC 1 cut(s) 821
BstAPI GCANNNNNTGC 1 cut(s) 670
BstBAI YACGTR 1 cut(s) 418
BstC8I GCNNGC 2 cut(s) 9, 903
BstDEI CTNAG 3 cut(s) 189, 543, 656
BstENI CCTNNNNNAGG 1 cut(s) 303
BstF5I GGATG 1 cut(s) 532
BstFNI CGCG 1 cut(s) 975
BstHHI GCGC 1 cut(s) 977
BstKTI GATC 1 cut(s) 750
BstMAI GTCTC 4 cut(s) 317, 478, 791, 923
BstMBI GATC 1 cut(s) 747
BstMWI GCNNNNNNNGC 4 cut(s) 229, 238, 670, 818
BstNI CCWGG 1 cut(s) 150
BstNSI RCATGY 1 cut(s) 685
BstSCI CCNGG 4 cut(s) 148, 223, 280, 821
BstSFI CTRYAG 2 cut(s) 18, 264
BstSLI GKGCMC 1 cut(s) 120
BstSNI TACGTA 1 cut(s) 418
BstUI CGCG 1 cut(s) 975
BstV1I GCAGC 4 cut(s) 23, 236, 930, 933
BstZ17I GTATAC 1 cut(s) 26
BsuRI GGCC 4 cut(s) 223, 309, 721, 818
BtsCI GGATG 1 cut(s) 532
BtsIMutI CAGTG 3 cut(s) 270, 442, 664
BveI ACCTGC 1 cut(s) 994
Cac8I GCNNGC 2 cut(s) 9, 903
CaiI CAGNNNCTG 1 cut(s) 17
CciI TCATGA 1 cut(s) 693
CfoI GCGC 1 cut(s) 977
Cfr13I GGNCC 4 cut(s) 222, 501, 719, 816
CseI GACGC 1 cut(s) 808
Csp6I GTAC 2 cut(s) 328, 596
CviAII CATG 9 cut(s) 242, 497, 606, 682, 686, 694, 736, 745, 835
CviQI GTAC 2 cut(s) 328, 596
DdeI CTNAG 3 cut(s) 189, 543, 656
DpnI GATC 1 cut(s) 749
DpnII GATC 1 cut(s) 747
Eam1104I CTCTTC 1 cut(s) 821
EarI CTCTTC 1 cut(s) 821
EciI GGCGGA 1 cut(s) 24
Eco105I TACGTA 1 cut(s) 418
Eco24I GRGCYC 2 cut(s) 234, 569
Eco31I GGTCTC 1 cut(s) 478
Eco32I GATATC 2 cut(s) 448, 988
Eco47I GGWCC 1 cut(s) 501
Eco57I CTGAAG 1 cut(s) 50
Eco88I CYCGRG 2 cut(s) 803, 813
EcoNI CCTNNNNNAGG 1 cut(s) 303
EcoRI GAATTC 2 cut(s) 275, 967
EcoRII CCWGG 1 cut(s) 148
EcoRV GATATC 2 cut(s) 448, 988
EcoT22I ATGCAT 1 cut(s) 687
EcoT38I GRGCYC 2 cut(s) 234, 569
Esp3I CGTCTC 2 cut(s) 317, 791
FaeI CATG 9 cut(s) 245, 500, 609, 685, 689, 697, 739, 748, 838
FalI AAGNNNNNCTT 2 cut(s) 698, 730
FaqI GGGAC 1 cut(s) 514
FatI CATG 9 cut(s) 241, 496, 605, 681, 685, 693, 735, 744, 834
FauI CCCGC 1 cut(s) 980
FbaI TGATCA 1 cut(s) 747
FblI GTMKAC 1 cut(s) 25
Fnu4HI GCNGC 7 cut(s) 12, 78, 250, 323, 819, 919, 922
FokI GGATG 1 cut(s) 539
FriOI GRGCYC 2 cut(s) 234, 569
Fsp4HI GCNGC 7 cut(s) 12, 78, 250, 323, 819, 919, 922
FspBI CTAG 2 cut(s) 723, 1098
GlaI GCGC 1 cut(s) 976
GluI GCNGC 7 cut(s) 12, 78, 250, 323, 819, 919, 922
HaeIII GGCC 4 cut(s) 223, 309, 721, 818
HapII CCGG 5 cut(s) 205, 225, 282, 765, 822
HgaI GACGC 1 cut(s) 808
HhaI GCGC 1 cut(s) 977
Hin1II CATG 9 cut(s) 245, 500, 609, 685, 689, 697, 739, 748, 838
Hin6I GCGC 1 cut(s) 975
HinP1I GCGC 1 cut(s) 975
HincII GTYRAC 1 cut(s) 287
HindII GTYRAC 1 cut(s) 287
HindIII AAGCTT 1 cut(s) 878
HinfI GANTC 6 cut(s) 185, 388, 493, 652, 829, 908
HpaII CCGG 5 cut(s) 205, 225, 282, 765, 822
HphI GGTGA 2 cut(s) 239, 637
Hpy166II GTNNAC 4 cut(s) 26, 287, 358, 945
Hpy188I TCNGA 7 cut(s) 85, 190, 295, 317, 397, 592, 847
Hpy188III TCNNGA 7 cut(s) 205, 490, 511, 619, 694, 751, 765
Hpy8I GTNNAC 4 cut(s) 26, 287, 358, 945
HpyAV CCTTC 4 cut(s) 71, 517, 546, 781
HpyCH4III ACNGT 4 cut(s) 19, 291, 587, 851
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 6 cut(s) 7, 103, 685, 865, 937, 1003
HpyF10VI GCNNNNNNNGC 4 cut(s) 229, 238, 670, 818
HpyF3I CTNAG 3 cut(s) 189, 543, 656
HpySE526I ACGT 1 cut(s) 417
Hsp92II CATG 9 cut(s) 245, 500, 609, 685, 689, 697, 739, 748, 838
HspAI GCGC 1 cut(s) 975
Kpn2I TCCGGA 2 cut(s) 204, 764
Ksp22I TGATCA 1 cut(s) 747
Kzo9I GATC 1 cut(s) 747
LmnI GCTCC 1 cut(s) 982
Lsp1109I GCAGC 4 cut(s) 23, 236, 930, 933
LweI GCATC 1 cut(s) 1005
MaeI CTAG 2 cut(s) 723, 1098
MaeII ACGT 1 cut(s) 417
MaeIII GTNAC 2 cut(s) 437, 830
MalI GATC 1 cut(s) 749
MboI GATC 1 cut(s) 747
MboII GAAGA 6 cut(s) 56, 358, 634, 718, 838, 1046
MfeI CAATTG 3 cut(s) 72, 377, 477
MhlI GDGCHC 3 cut(s) 120, 234, 569
MluCI AATT 9 cut(s) 72, 178, 275, 331, 377, 430, 477, 768, 967
MlyI GAGTC 4 cut(s) 194, 382, 487, 838
MnlI CCTC 4 cut(s) 550, 586, 747, 1024
Mph1103I ATGCAT 1 cut(s) 687
MroI TCCGGA 2 cut(s) 204, 764
MroXI GAANNNNTTC 1 cut(s) 586
MseI TTAA 4 cut(s) 195, 1011, 1059, 1102
MslI CAYNNNNRTG 1 cut(s) 240
MspA1I CMGCKG 1 cut(s) 249
MspI CCGG 5 cut(s) 205, 225, 282, 765, 822
MspR9I CCNGG 4 cut(s) 150, 225, 282, 823
MunI CAATTG 3 cut(s) 72, 377, 477
MvaI CCWGG 1 cut(s) 150
MvnI CGCG 1 cut(s) 975
MwoI GCNNNNNNNGC 4 cut(s) 229, 238, 670, 818
NciI CCSGG 3 cut(s) 225, 282, 823
NdeII GATC 1 cut(s) 747
NlaIII CATG 9 cut(s) 245, 500, 609, 685, 689, 697, 739, 748, 838
NlaIV GGNNCC 2 cut(s) 117, 502
NmuCI GTSAC 2 cut(s) 437, 830
NsiI ATGCAT 1 cut(s) 687
NspI RCATGY 1 cut(s) 685
PagI TCATGA 1 cut(s) 693
PdmI GAANNNNTTC 1 cut(s) 586
PfeI GAWTC 2 cut(s) 652, 908
PflMI CCANNNNNTGG 1 cut(s) 149
PkrI GCNGC 7 cut(s) 13, 79, 251, 324, 820, 920, 923
PleI GAGTC 4 cut(s) 193, 382, 487, 837
PpsI GAGTC 4 cut(s) 193, 382, 487, 837
Ppu21I YACGTR 1 cut(s) 418
PsiI TTATAA 2 cut(s) 884, 1022
Psp6I CCWGG 1 cut(s) 148
PspGI CCWGG 1 cut(s) 148
PspN4I GGNNCC 2 cut(s) 117, 502
PspPI GGNCC 4 cut(s) 222, 501, 719, 816
PstNI CAGNNNCTG 1 cut(s) 17
RsaI GTAC 2 cut(s) 329, 597
RsaNI GTAC 2 cut(s) 328, 596
RseI CAYNNNNRTG 1 cut(s) 240
SaqAI TTAA 4 cut(s) 195, 1011, 1059, 1102
SatI GCNGC 7 cut(s) 12, 78, 250, 323, 819, 919, 922
Sau3AI GATC 1 cut(s) 747
Sau96I GGNCC 4 cut(s) 222, 501, 719, 816
SchI GAGTC 4 cut(s) 194, 382, 487, 838
ScrFI CCNGG 4 cut(s) 150, 225, 282, 823
SduI GDGCHC 3 cut(s) 120, 234, 569
SfaNI GCATC 1 cut(s) 1005
SfcI CTRYAG 2 cut(s) 18, 264
SinI GGWCC 1 cut(s) 501
SmiMI CAYNNNNRTG 1 cut(s) 240
SmlI CTYRAG 3 cut(s) 488, 875, 1028
SmoI CTYRAG 3 cut(s) 488, 875, 1028
SnaBI TACGTA 1 cut(s) 418
Sse9I AATT 9 cut(s) 72, 178, 275, 331, 377, 430, 477, 768, 967
SsiI CCGC 6 cut(s) 35, 78, 247, 323, 819, 973
SspMI CTAG 2 cut(s) 723, 1098
StyD4I CCNGG 4 cut(s) 148, 223, 280, 821
TaaI ACNGT 4 cut(s) 19, 291, 587, 851
TaiI ACGT 1 cut(s) 420
TaqI TCGA 1 cut(s) 450
TaqII GACCGA 1 cut(s) 518
TasI AATT 9 cut(s) 72, 178, 275, 331, 377, 430, 477, 768, 967
TatI WGTACW 1 cut(s) 327
TauI GCSGC 3 cut(s) 80, 325, 821
TfiI GAWTC 2 cut(s) 652, 908
Tru1I TTAA 4 cut(s) 195, 1011, 1059, 1102
Tru9I TTAA 4 cut(s) 195, 1011, 1059, 1102
TscAI CASTG 3 cut(s) 277, 442, 664
TseFI GTSAC 2 cut(s) 437, 830
TseI GCWGC 4 cut(s) 11, 249, 918, 921
Tsp45I GTSAC 2 cut(s) 437, 830
TspDTI ATGAA 4 cut(s) 359, 682, 752, 965
TspRI CASTG 3 cut(s) 277, 442, 664
Van91I CCANNNNNTGG 1 cut(s) 149
VpaK11BI GGWCC 1 cut(s) 501
XagI CCTNNNNNAGG 1 cut(s) 303
XapI RAATTY 4 cut(s) 178, 275, 768, 967
XceI RCATGY 1 cut(s) 685
XmiI GTMKAC 1 cut(s) 25
XmnI GAANNNNTTC 1 cut(s) 586
XspI CTAG 2 cut(s) 723, 1098
Zsp2I ATGCAT 1 cut(s) 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.