Rh6DG133000

CCR4-associated factor 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000757
Physical Location & Seq
Forward (+)
18942814 .. 18943869
1056 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG133000.1

Sequence Viewer

Length: 1056 bp
ATGATAGTTCAGAGAGGAATTCCGATCTTCCCAATTGGGCCTGCTTCTCATTTCCACGGCGGAGTTCCGGTGGTCTTCCACAGCCGACCTGTGCCTCCGATTCCTCTACACCACCGCCCTATACCCCTTTATCCTCCTCAATTTCAGAGCCGCGTCCCTGTTTTCCACGTTGTCGGCGCCCCAATTTGTCGTCAAGGATCTGTGAAGAAAGCCGATCGGTTCGAGGTTCGAAGCGTTTGTCGCTATAATTTCAAAGCCGAGATTGCCGTAATTCAGAGTCTAATCAGGGCCGGCTACCGATTTGCTAGTTTCGACACCGAATTCCCCGGGACAGTGGTTAAATCCGAAATTCCGAAACATCTGATATCTCAAGCTGTTCCGGTTGATGTGTATAAGATGATGAAGACCAATGTGGACCAAACCCATATTATCCAATTGGGTTTTTCACTCTCTGACCCATTTGGGAATTTGCCGGTTTATGATGGTGCTTACTGTTGTTGGGAGTTTAATTTCAATGATTTTGATGTGGACTGTGCTCAACATTTGCAGAATAGTGTCTCGATTGATGTCCTGAAGCGGCAAGGAATAGACTTTTCCGAAAACAAATGCACTGGTATTTGTTCTGCAGACTTTGCTGAGGAGATTGAGAGGTCTGGGCTAGTGGAGTTGCTTCCTTGCTTGACTTGGGCTACCTTCCAGAGTGCATATGATTTTGGGTACTTGATAAAGATGTTCACTGGGAACAAGGAATTGCCAGAGGATATCAACGAGTTCATGGGCAATGTGAAGACATATTTTGGACCGAATGTGTACGACATCAAGTACATGATGAAGTTCTGTGATGGACTGTTTGGCGGTTTGAATAGCGTGGCAGAAACCCTTGGCGTTGACCGTGTGGCTGGGAGTAGCCACCAGGCTGGTTCTGATAGTCTTTTGACACTGCAAACGTTTGTCAAGATGCTCAAGATGGAGAAGAACAAAGGGAAAGAGCTCTGGGAAGAATGTGGAGGAGTTTTGTTTGGTTTAGGAAGCTTGGATTTCTGGCATCATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1 RVT_3

Protein Analysis

351

Amino Acids

39.35

Weight (kDa)

6.18

Isoelectric Point (pI)

45.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 79 - 207 4.8e-08 CAF1 family ribonuclease
CAF1 PF04857 235 - 279 2.9e-06 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 176
AccII CGCG 1 cut(s) 153
AciI CCGC 5 cut(s) 60, 115, 151, 577, 855
AclI AACGTT 1 cut(s) 947
AclWI GGATC 1 cut(s) 205
AcsI RAATTY 4 cut(s) 18, 320, 348, 466
AcuI CTGAAG 1 cut(s) 593
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 3 cut(s) 719, 812, 824
AgsI TTSAA 3 cut(s) 253, 514, 862
AjnI CCWGG 1 cut(s) 912
AluBI AGCT 3 cut(s) 374, 991, 1032
AluI AGCT 3 cut(s) 374, 991, 1032
Alw21I GWGCWC 2 cut(s) 538, 993
Alw26I GTCTC 1 cut(s) 562
AlwI GGATC 1 cut(s) 205
Ama87I CYCGRG 1 cut(s) 326
AoxI GGCC 2 cut(s) 38, 288
ApoI RAATTY 4 cut(s) 18, 320, 348, 466
ArsI GACNNNNNNTTYG 2 cut(s) 223, 255
AspLEI GCGC 1 cut(s) 179
AspS9I GGNCC 4 cut(s) 38, 288, 415, 800
AsuC2I CCSGG 2 cut(s) 327, 328
AsuII TTCGAA 1 cut(s) 229
AvaI CYCGRG 1 cut(s) 326
AvaII GGWCC 2 cut(s) 415, 800
BanI GGYRCC 1 cut(s) 176
BanII GRGCYC 1 cut(s) 993
BbsI GAAGAC 3 cut(s) 67, 410, 794
Bbv12I GWGCWC 2 cut(s) 538, 993
BbvCI CCTCAGC 1 cut(s) 636
BccI CCATC 3 cut(s) 476, 836, 961
BceAI ACGGC 2 cut(s) 73, 251
BciT130I CCWGG 1 cut(s) 914
BcnI CCSGG 2 cut(s) 327, 328
BcoDI GTCTC 1 cut(s) 562
BfaI CTAG 2 cut(s) 306, 659
BfmI CTRYAG 1 cut(s) 624
BfoI RGCGCY 1 cut(s) 180
BisI GCNGC 2 cut(s) 151, 578
BlsI GCNGC 2 cut(s) 152, 579
Bme1390I CCNGG 3 cut(s) 327, 328, 914
Bme18I GGWCC 2 cut(s) 415, 800
BmeT110I CYCGRG 1 cut(s) 326
BmgT120I GGNCC 4 cut(s) 38, 288, 415, 800
BmiI GGNNCC 1 cut(s) 178
BmrFI CCNGG 3 cut(s) 327, 328, 914
BmrI ACTGGG 1 cut(s) 747
BmsI GCATC 1 cut(s) 948
BmuI ACTGGG 1 cut(s) 747
BpiI GAAGAC 3 cut(s) 67, 410, 794
Bpu10I CCTNAGC 1 cut(s) 636
Bpu14I TTCGAA 1 cut(s) 229
BpuEI CTTGAG 2 cut(s) 354, 947
BpuMI CCSGG 2 cut(s) 327, 328
BsaHI GRCGYC 1 cut(s) 177
BsaJI CCNNGG 4 cut(s) 55, 325, 326, 880
BsaWI WCCGGW 2 cut(s) 67, 379
BsaXI ACNNNNNCTCC 2 cut(s) 54, 84
Bse118I RCCGGY 2 cut(s) 290, 472
Bse1I ACTGG 2 cut(s) 616, 742
Bse3DI GCAATG 1 cut(s) 787
BseBI CCWGG 1 cut(s) 914
BseDI CCNNGG 4 cut(s) 55, 325, 326, 880
BseMI GCAATG 1 cut(s) 787
BseMII CTCAG 1 cut(s) 627
BseNI ACTGG 2 cut(s) 616, 742
BseRI GAGGAG 3 cut(s) 126, 653, 1023
BseYI CCCAGC 1 cut(s) 899
Bsh1236I CGCG 1 cut(s) 153
Bsh1285I CGRYCG 1 cut(s) 217
BshFI GGCC 2 cut(s) 40, 290
BshNI GGYRCC 1 cut(s) 176
BsiEI CGRYCG 1 cut(s) 217
BsiHKAI GWGCWC 2 cut(s) 538, 993
BsiHKCI CYCGRG 1 cut(s) 326
BsiSI CCGG 5 cut(s) 68, 291, 327, 380, 473
BslFI GGGAC 2 cut(s) 140, 343
BsmAI GTCTC 1 cut(s) 562
BsmFI GGGAC 2 cut(s) 140, 343
BsnI GGCC 2 cut(s) 40, 290
BsoBI CYCGRG 1 cut(s) 326
Bsp119I TTCGAA 1 cut(s) 229
Bsp1286I GDGCHC 2 cut(s) 538, 993
Bsp143I GATC 3 cut(s) 24, 197, 214
BspACI CCGC 5 cut(s) 60, 115, 151, 577, 855
BspANI GGCC 2 cut(s) 40, 290
BspCNI CTCAG 1 cut(s) 628
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 1 cut(s) 178
BspMAI CTGCAG 1 cut(s) 628
BspPI GGATC 1 cut(s) 205
BspT104I TTCGAA 1 cut(s) 229
BspT107I GGYRCC 1 cut(s) 176
BsrDI GCAATG 1 cut(s) 787
BsrFI RCCGGY 2 cut(s) 290, 472
BsrI ACTGG 2 cut(s) 616, 742
BssAI RCCGGY 2 cut(s) 290, 472
BssECI CCNNGG 4 cut(s) 55, 325, 326, 880
BssMI GATC 3 cut(s) 24, 197, 214
BssNI GRCGYC 1 cut(s) 177
BssT1I CCWWGG 1 cut(s) 880
Bst2UI CCWGG 1 cut(s) 914
Bst4CI ACNGT 5 cut(s) 334, 494, 533, 849, 893
BstACI GRCGYC 1 cut(s) 177
BstAPI GCANNNNNTGC 1 cut(s) 632
BstBI TTCGAA 1 cut(s) 229
BstC8I GCNNGC 2 cut(s) 42, 292
BstDEI CTNAG 1 cut(s) 636
BstDSI CCRYGG 1 cut(s) 55
BstFNI CGCG 1 cut(s) 153
BstH2I RGCGCY 1 cut(s) 180
BstHHI GCGC 1 cut(s) 179
BstKTI GATC 3 cut(s) 27, 200, 217
BstMAI GTCTC 1 cut(s) 562
BstMBI GATC 3 cut(s) 24, 197, 214
BstMCI CGRYCG 1 cut(s) 217
BstMWI GCNNNNNNNGC 3 cut(s) 240, 263, 632
BstNI CCWGG 1 cut(s) 914
BstSCI CCNGG 3 cut(s) 325, 326, 912
BstSFI CTRYAG 1 cut(s) 624
BstUI CGCG 1 cut(s) 153
BstV2I GAAGAC 3 cut(s) 67, 410, 794
BstX2I RGATCY 1 cut(s) 197
BstXI CCANNNNNNTGG 1 cut(s) 917
BstYI RGATCY 1 cut(s) 197
BsuRI GGCC 2 cut(s) 40, 290
BtgI CCRYGG 1 cut(s) 55
BtsI GCAGTG 1 cut(s) 938
BtsIMutI CAGTG 4 cut(s) 339, 609, 735, 938
Cac8I GCNNGC 2 cut(s) 42, 292
CfoI GCGC 1 cut(s) 179
Cfr10I RCCGGY 2 cut(s) 290, 472
Cfr13I GGNCC 4 cut(s) 38, 288, 415, 800
Cfr9I CCCGGG 1 cut(s) 326
CseI GACGC 1 cut(s) 142
Csp6I GTAC 3 cut(s) 718, 811, 823
CviAII CATG 2 cut(s) 775, 826
CviQI GTAC 3 cut(s) 718, 811, 823
DdeI CTNAG 1 cut(s) 636
DinI GGCGCC 1 cut(s) 178
DpnI GATC 3 cut(s) 26, 199, 216
DpnII GATC 3 cut(s) 24, 197, 214
EciI GGCGGA 1 cut(s) 75
Ecl136II GAGCTC 1 cut(s) 991
Eco130I CCWWGG 1 cut(s) 880
Eco24I GRGCYC 1 cut(s) 993
Eco32I GATATC 2 cut(s) 366, 763
Eco47I GGWCC 2 cut(s) 415, 800
Eco53kI GAGCTC 1 cut(s) 991
Eco57I CTGAAG 1 cut(s) 593
Eco88I CYCGRG 1 cut(s) 326
EcoICRI GAGCTC 1 cut(s) 991
EcoRI GAATTC 2 cut(s) 18, 320
EcoRII CCWGG 1 cut(s) 912
EcoRV GATATC 2 cut(s) 366, 763
EcoT14I CCWWGG 1 cut(s) 880
EcoT38I GRGCYC 1 cut(s) 993
EgeI GGCGCC 1 cut(s) 178
EheI GGCGCC 1 cut(s) 178
ErhI CCWWGG 1 cut(s) 880
FaeI CATG 2 cut(s) 778, 829
FaqI GGGAC 2 cut(s) 140, 343
FatI CATG 2 cut(s) 774, 825
FauNDI CATATG 1 cut(s) 706
Fnu4HI GCNGC 2 cut(s) 151, 578
FriOI GRGCYC 1 cut(s) 993
Fsp4HI GCNGC 2 cut(s) 151, 578
FspBI CTAG 2 cut(s) 306, 659
GlaI GCGC 1 cut(s) 178
GluI GCNGC 2 cut(s) 151, 578
GsaI CCCAGC 1 cut(s) 903
HaeII RGCGCY 1 cut(s) 180
HaeIII GGCC 2 cut(s) 40, 290
HapII CCGG 5 cut(s) 68, 291, 327, 380, 473
HgaI GACGC 1 cut(s) 142
HhaI GCGC 1 cut(s) 179
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 2 cut(s) 778, 829
Hin6I GCGC 1 cut(s) 177
HinP1I GCGC 1 cut(s) 177
HincII GTYRAC 1 cut(s) 889
HindII GTYRAC 1 cut(s) 889
HindIII AAGCTT 1 cut(s) 1030
HinfI GANTC 2 cut(s) 100, 277
HpaII CCGG 5 cut(s) 68, 291, 327, 380, 473
Hpy166II GTNNAC 5 cut(s) 415, 529, 735, 811, 889
Hpy188III TCNNGA 5 cut(s) 559, 571, 697, 955, 964
Hpy8I GTNNAC 5 cut(s) 415, 529, 735, 811, 889
HpyAV CCTTC 1 cut(s) 703
HpyCH4III ACNGT 5 cut(s) 334, 494, 533, 849, 893
HpyCH4IV ACGT 2 cut(s) 168, 947
HpyCH4V TGCA 5 cut(s) 547, 609, 626, 704, 943
HpyF10VI GCNNNNNNNGC 3 cut(s) 240, 263, 632
HpyF3I CTNAG 1 cut(s) 636
HpySE526I ACGT 2 cut(s) 168, 947
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 2 cut(s) 778, 829
HspAI GCGC 1 cut(s) 177
KasI GGCGCC 1 cut(s) 176
KroI GCCGGC 1 cut(s) 290
KroNI GCCGGC 1 cut(s) 292
Kzo9I GATC 3 cut(s) 24, 197, 214
LweI GCATC 1 cut(s) 948
MaeI CTAG 2 cut(s) 306, 659
MaeII ACGT 2 cut(s) 168, 947
MalI GATC 3 cut(s) 26, 199, 216
MboI GATC 3 cut(s) 24, 197, 214
MboII GAAGA 7 cut(s) 19, 67, 217, 415, 799, 985, 1010
MfeI CAATTG 2 cut(s) 33, 434
MflI RGATCY 1 cut(s) 197
MhlI GDGCHC 2 cut(s) 538, 993
Mly113I GGCGCC 1 cut(s) 177
MlyI GAGTC 1 cut(s) 286
MroNI GCCGGC 1 cut(s) 290
MseI TTAA 2 cut(s) 339, 507
MspI CCGG 5 cut(s) 68, 291, 327, 380, 473
MspR9I CCNGG 3 cut(s) 327, 328, 914
MunI CAATTG 2 cut(s) 33, 434
MvaI CCWGG 1 cut(s) 914
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 3 cut(s) 240, 263, 632
NaeI GCCGGC 1 cut(s) 292
NarI GGCGCC 1 cut(s) 177
NciI CCSGG 2 cut(s) 327, 328
NdeI CATATG 1 cut(s) 706
NdeII GATC 3 cut(s) 24, 197, 214
NgoMIV GCCGGC 1 cut(s) 290
NlaIII CATG 2 cut(s) 778, 829
NlaIV GGNNCC 1 cut(s) 178
NmeAIII GCCGAG 1 cut(s) 283
NspV TTCGAA 1 cut(s) 229
PdiI GCCGGC 1 cut(s) 292
PfeI GAWTC 1 cut(s) 100
PkrI GCNGC 2 cut(s) 152, 579
Ple19I CGATCG 1 cut(s) 217
PleI GAGTC 1 cut(s) 285
PluTI GGCGCC 1 cut(s) 180
PpsI GAGTC 1 cut(s) 285
Psp124BI GAGCTC 1 cut(s) 993
Psp1406I AACGTT 1 cut(s) 947
Psp6I CCWGG 1 cut(s) 912
PspFI CCCAGC 1 cut(s) 899
PspGI CCWGG 1 cut(s) 912
PspN4I GGNNCC 1 cut(s) 178
PspPI GGNCC 4 cut(s) 38, 288, 415, 800
PstI CTGCAG 1 cut(s) 628
PsuI RGATCY 1 cut(s) 197
PvuI CGATCG 1 cut(s) 217
RsaI GTAC 3 cut(s) 719, 812, 824
RsaNI GTAC 3 cut(s) 718, 811, 823
SacI GAGCTC 1 cut(s) 993
SaqAI TTAA 2 cut(s) 339, 507
SatI GCNGC 2 cut(s) 151, 578
Sau3AI GATC 3 cut(s) 24, 197, 214
Sau96I GGNCC 4 cut(s) 38, 288, 415, 800
SchI GAGTC 1 cut(s) 286
ScrFI CCNGG 3 cut(s) 327, 328, 914
SduI GDGCHC 2 cut(s) 538, 993
SetI ASST 9 cut(s) 91, 171, 228, 376, 653, 695, 950, 993, 1034
SfaNI GCATC 1 cut(s) 948
SfcI CTRYAG 1 cut(s) 624
SfoI GGCGCC 1 cut(s) 178
SfuI TTCGAA 1 cut(s) 229
SinI GGWCC 2 cut(s) 415, 800
SmaI CCCGGG 1 cut(s) 328
SmlI CTYRAG 2 cut(s) 369, 962
SmoI CTYRAG 2 cut(s) 369, 962
SsiI CCGC 5 cut(s) 60, 115, 151, 577, 855
SspDI GGCGCC 1 cut(s) 176
SspMI CTAG 2 cut(s) 306, 659
SstI GAGCTC 1 cut(s) 993
StyD4I CCNGG 3 cut(s) 325, 326, 912
StyI CCWWGG 1 cut(s) 880
TaaI ACNGT 5 cut(s) 334, 494, 533, 849, 893
TaiI ACGT 2 cut(s) 171, 950
TaqI TCGA 4 cut(s) 222, 229, 312, 560
TaqII GACCGA 1 cut(s) 817
TatI WGTACW 1 cut(s) 822
TauI GCSGC 2 cut(s) 153, 580
TfiI GAWTC 1 cut(s) 100
Tru1I TTAA 2 cut(s) 339, 507
Tru9I TTAA 2 cut(s) 339, 507
TscAI CASTG 4 cut(s) 339, 616, 742, 945
TspDTI ATGAA 3 cut(s) 416, 763, 845
TspMI CCCGGG 1 cut(s) 326
TspRI CASTG 4 cut(s) 339, 616, 742, 945
VpaK11BI GGWCC 2 cut(s) 415, 800
XapI RAATTY 4 cut(s) 18, 320, 348, 466
XmaI CCCGGG 1 cut(s) 326
XspI CTAG 2 cut(s) 306, 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.