Rh2DG408200

CCR4-associated factor 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000757
Physical Location & Seq
Forward (+)
59175022 .. 59176077
1056 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG408200.1

Sequence Viewer

Length: 1056 bp
ATGATAGTTCAGAGAGGAACTCCGATCTTCCCAGTTGGGCCTGCTTCTCACTTCCACGGCGGAGTTCCGGTGGTCTTCCACCGCCGACCTATGCCTCAGATTCCTCTACACCACCGCCCTATACCCCTTTATCCTCCTCAATTTCAGAGCCGCGTCCCTGTTTTCCGGGTTGTCGGCGCCCCAATTTGTCGTCAAGGATCTGTGAAGAAAGCCGATCGGTTCGAGGTTCGAAGTGTTTGTCGCTATAATTTCAAAGCCGAGATTGCCGTAATTCAGAGTCTAATCAGGGCCGGCTACCGATTTGCTAGTTTCGACACCGAATTCCCCGGGACAGTGGTTAAATCCGAAATTCCGAAACATCTGATATCTCAAGCTGTTCCGGTTGATGTGTATAAGATGATGAAGACCAATGTGGACCAAACCCATATTATCCAATTGGGTCTTTCACTCTCTGACCCATGTGGGAATTTGCCGGTTTATGATGGTGCGTACTGCTGTTGGGAGTTTAATTTCAGTGATTTTGATGTAGACTGTGAAAAACATTTGCGGAATAGTGACTCAATTGACCTCCTGAAGCGGCAAGGAATAGACTTTTCCGAAAACAAATGCACTGGTATTTGTTCTGCAGACTTTGCTGAGGAGATTGAGAGGTCCGGGCTAGTGGAGTTGCTTCCTTGCTTGACTTGGGCTACTTTCCAGAGTGCATATGATTTTGGGTACTTGATAAAGATGTTCACTGGGAACAAGGAATTGCCAGAGGATATCAAGGAGTTCATGGGCAATGTGAAGACATATTTTGGACCGAATGTGTACGACATCAAGTACATGATGAAGTTCTGTGATGGACTGTTTGGCGGTTTGAATAGCGTGGCAGCAACCCTTGGCGTTGACCGTGTGGCTGGGAGTAGCCACCAGGCTGGTTCTGATAGTCTTTTGACACTGCAGATGTTTGTCAAGATGCTCAAGATGGAGAAGAACAAAGGGAAAGAGCTCTGGGAAGAATGCGGAGGAGTTTTGTTTGGTTTAGGAAGCTTGGATTTCTGGCATCATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1 RVT_3

Protein Analysis

351

Amino Acids

39.48

Weight (kDa)

6.82

Isoelectric Point (pI)

46.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 79 - 207 2.3e-09 CAF1 family ribonuclease
CAF1 PF04857 235 - 278 3.3e-06 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 176
AccI GTMKAC 1 cut(s) 528
AccII CGCG 1 cut(s) 153
AciI CCGC 8 cut(s) 60, 82, 115, 151, 547, 577, 855, 1005
AclWI GGATC 1 cut(s) 205
AcsI RAATTY 3 cut(s) 320, 348, 466
AcuI CTGAAG 1 cut(s) 593
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 4 cut(s) 491, 719, 812, 824
AgsI TTSAA 2 cut(s) 253, 862
AjnI CCWGG 1 cut(s) 912
AluBI AGCT 3 cut(s) 374, 991, 1032
AluI AGCT 3 cut(s) 374, 991, 1032
Alw21I GWGCWC 1 cut(s) 993
AlwI GGATC 1 cut(s) 205
Ama87I CYCGRG 1 cut(s) 326
AoxI GGCC 2 cut(s) 38, 288
ApeKI GCWGC 1 cut(s) 872
ApoI RAATTY 3 cut(s) 320, 348, 466
ArsI GACNNNNNNTTYG 2 cut(s) 223, 255
AspLEI GCGC 1 cut(s) 179
AspS9I GGNCC 5 cut(s) 38, 288, 415, 651, 800
AsuC2I CCSGG 4 cut(s) 167, 327, 328, 655
AsuII TTCGAA 1 cut(s) 229
AvaI CYCGRG 1 cut(s) 326
AvaII GGWCC 3 cut(s) 415, 651, 800
BanI GGYRCC 1 cut(s) 176
BanII GRGCYC 1 cut(s) 993
BbsI GAAGAC 3 cut(s) 67, 410, 794
Bbv12I GWGCWC 1 cut(s) 993
BbvCI CCTCAGC 1 cut(s) 636
BbvI GCAGC 1 cut(s) 884
BccI CCATC 3 cut(s) 476, 836, 961
BceAI ACGGC 2 cut(s) 73, 251
BciT130I CCWGG 1 cut(s) 914
BcnI CCSGG 4 cut(s) 167, 327, 328, 655
BfaI CTAG 2 cut(s) 306, 659
BfmI CTRYAG 2 cut(s) 624, 941
BfoI RGCGCY 1 cut(s) 180
BisI GCNGC 3 cut(s) 151, 578, 873
BlsI GCNGC 3 cut(s) 152, 579, 874
Bme1390I CCNGG 5 cut(s) 167, 327, 328, 655, 914
Bme18I GGWCC 3 cut(s) 415, 651, 800
BmeT110I CYCGRG 1 cut(s) 326
BmgT120I GGNCC 5 cut(s) 38, 288, 415, 651, 800
BmiI GGNNCC 1 cut(s) 178
BmrFI CCNGG 5 cut(s) 167, 327, 328, 655, 914
BmrI ACTGGG 2 cut(s) 26, 747
BmsI GCATC 1 cut(s) 948
BmuI ACTGGG 2 cut(s) 26, 747
BpiI GAAGAC 3 cut(s) 67, 410, 794
BplI GAGNNNNNCTC 1 cut(s) 36
Bpu10I CCTNAGC 1 cut(s) 636
Bpu14I TTCGAA 1 cut(s) 229
BpuEI CTTGAG 2 cut(s) 354, 947
BpuMI CCSGG 4 cut(s) 167, 327, 328, 655
BsaHI GRCGYC 1 cut(s) 177
BsaJI CCNNGG 4 cut(s) 55, 325, 326, 880
BsaWI WCCGGW 2 cut(s) 67, 379
BsaXI ACNNNNNCTCC 2 cut(s) 54, 84
Bse118I RCCGGY 2 cut(s) 290, 472
Bse1I ACTGG 3 cut(s) 32, 616, 742
Bse3DI GCAATG 1 cut(s) 787
BseBI CCWGG 1 cut(s) 914
BseDI CCNNGG 4 cut(s) 55, 325, 326, 880
BseMI GCAATG 1 cut(s) 787
BseMII CTCAG 2 cut(s) 110, 627
BseNI ACTGG 3 cut(s) 32, 616, 742
BseRI GAGGAG 3 cut(s) 126, 653, 1023
BseXI GCAGC 1 cut(s) 884
BseYI CCCAGC 1 cut(s) 899
Bsh1236I CGCG 1 cut(s) 153
Bsh1285I CGRYCG 1 cut(s) 217
BshFI GGCC 2 cut(s) 40, 290
BshNI GGYRCC 1 cut(s) 176
BsiEI CGRYCG 1 cut(s) 217
BsiHKAI GWGCWC 1 cut(s) 993
BsiHKCI CYCGRG 1 cut(s) 326
BsiSI CCGG 7 cut(s) 68, 166, 291, 327, 380, 473, 654
BslFI GGGAC 2 cut(s) 140, 343
BsmFI GGGAC 2 cut(s) 140, 343
BsmI GAATGC 1 cut(s) 1007
BsnI GGCC 2 cut(s) 40, 290
BsoBI CYCGRG 1 cut(s) 326
Bsp119I TTCGAA 1 cut(s) 229
Bsp1286I GDGCHC 1 cut(s) 993
Bsp143I GATC 3 cut(s) 24, 197, 214
BspACI CCGC 8 cut(s) 60, 82, 115, 151, 547, 577, 855, 1005
BspANI GGCC 2 cut(s) 40, 290
BspCNI CTCAG 2 cut(s) 109, 628
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 1 cut(s) 178
BspMAI CTGCAG 2 cut(s) 628, 945
BspPI GGATC 1 cut(s) 205
BspT104I TTCGAA 1 cut(s) 229
BspT107I GGYRCC 1 cut(s) 176
BsrDI GCAATG 1 cut(s) 787
BsrFI RCCGGY 2 cut(s) 290, 472
BsrI ACTGG 3 cut(s) 32, 616, 742
BssAI RCCGGY 2 cut(s) 290, 472
BssECI CCNNGG 4 cut(s) 55, 325, 326, 880
BssMI GATC 3 cut(s) 24, 197, 214
BssNI GRCGYC 1 cut(s) 177
BssT1I CCWWGG 1 cut(s) 880
Bst2UI CCWGG 1 cut(s) 914
Bst4CI ACNGT 4 cut(s) 334, 533, 849, 893
BstACI GRCGYC 1 cut(s) 177
BstAPI GCANNNNNTGC 1 cut(s) 632
BstBI TTCGAA 1 cut(s) 229
BstC8I GCNNGC 2 cut(s) 42, 292
BstDEI CTNAG 2 cut(s) 96, 636
BstDSI CCRYGG 1 cut(s) 55
BstFNI CGCG 1 cut(s) 153
BstH2I RGCGCY 1 cut(s) 180
BstHHI GCGC 1 cut(s) 179
BstKTI GATC 3 cut(s) 27, 200, 217
BstMBI GATC 3 cut(s) 24, 197, 214
BstMCI CGRYCG 1 cut(s) 217
BstMWI GCNNNNNNNGC 2 cut(s) 263, 632
BstNI CCWGG 1 cut(s) 914
BstSCI CCNGG 5 cut(s) 165, 325, 326, 653, 912
BstSFI CTRYAG 2 cut(s) 624, 941
BstUI CGCG 1 cut(s) 153
BstV1I GCAGC 1 cut(s) 884
BstV2I GAAGAC 3 cut(s) 67, 410, 794
BstX2I RGATCY 1 cut(s) 197
BstXI CCANNNNNNTGG 1 cut(s) 917
BstYI RGATCY 1 cut(s) 197
BsuRI GGCC 2 cut(s) 40, 290
BtgI CCRYGG 1 cut(s) 55
BtsI GCAGTG 1 cut(s) 938
BtsIMutI CAGTG 5 cut(s) 339, 520, 609, 735, 938
Cac8I GCNNGC 2 cut(s) 42, 292
CfoI GCGC 1 cut(s) 179
Cfr10I RCCGGY 2 cut(s) 290, 472
Cfr13I GGNCC 5 cut(s) 38, 288, 415, 651, 800
Cfr9I CCCGGG 1 cut(s) 326
CseI GACGC 1 cut(s) 142
Csp6I GTAC 4 cut(s) 490, 718, 811, 823
CviAII CATG 3 cut(s) 459, 775, 826
CviQI GTAC 4 cut(s) 490, 718, 811, 823
DdeI CTNAG 2 cut(s) 96, 636
DinI GGCGCC 1 cut(s) 178
DpnI GATC 3 cut(s) 26, 199, 216
DpnII GATC 3 cut(s) 24, 197, 214
EciI GGCGGA 1 cut(s) 75
Ecl136II GAGCTC 1 cut(s) 991
Eco130I CCWWGG 1 cut(s) 880
Eco24I GRGCYC 1 cut(s) 993
Eco32I GATATC 2 cut(s) 366, 763
Eco47I GGWCC 3 cut(s) 415, 651, 800
Eco53kI GAGCTC 1 cut(s) 991
Eco57I CTGAAG 1 cut(s) 593
Eco88I CYCGRG 1 cut(s) 326
EcoICRI GAGCTC 1 cut(s) 991
EcoRI GAATTC 1 cut(s) 320
EcoRII CCWGG 1 cut(s) 912
EcoRV GATATC 2 cut(s) 366, 763
EcoT14I CCWWGG 1 cut(s) 880
EcoT38I GRGCYC 1 cut(s) 993
EgeI GGCGCC 1 cut(s) 178
EheI GGCGCC 1 cut(s) 178
ErhI CCWWGG 1 cut(s) 880
FaeI CATG 3 cut(s) 462, 778, 829
FaqI GGGAC 2 cut(s) 140, 343
FatI CATG 3 cut(s) 458, 774, 825
FauNDI CATATG 1 cut(s) 706
FblI GTMKAC 1 cut(s) 528
Fnu4HI GCNGC 3 cut(s) 151, 578, 873
FriOI GRGCYC 1 cut(s) 993
Fsp4HI GCNGC 3 cut(s) 151, 578, 873
FspBI CTAG 2 cut(s) 306, 659
GlaI GCGC 1 cut(s) 178
GluI GCNGC 3 cut(s) 151, 578, 873
GsaI CCCAGC 1 cut(s) 903
HaeII RGCGCY 1 cut(s) 180
HaeIII GGCC 2 cut(s) 40, 290
HapII CCGG 7 cut(s) 68, 166, 291, 327, 380, 473, 654
HgaI GACGC 1 cut(s) 142
HhaI GCGC 1 cut(s) 179
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 3 cut(s) 462, 778, 829
Hin6I GCGC 1 cut(s) 177
HinP1I GCGC 1 cut(s) 177
HincII GTYRAC 1 cut(s) 889
HindII GTYRAC 1 cut(s) 889
HindIII AAGCTT 1 cut(s) 1030
HinfI GANTC 3 cut(s) 100, 277, 557
HpaII CCGG 7 cut(s) 68, 166, 291, 327, 380, 473, 654
Hpy166II GTNNAC 5 cut(s) 415, 529, 735, 811, 889
Hpy188III TCNNGA 4 cut(s) 571, 697, 955, 964
Hpy8I GTNNAC 5 cut(s) 415, 529, 735, 811, 889
HpyCH4III ACNGT 4 cut(s) 334, 533, 849, 893
HpyCH4V TGCA 4 cut(s) 609, 626, 704, 943
HpyF10VI GCNNNNNNNGC 2 cut(s) 263, 632
HpyF3I CTNAG 2 cut(s) 96, 636
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 3 cut(s) 462, 778, 829
HspAI GCGC 1 cut(s) 177
KasI GGCGCC 1 cut(s) 176
KroI GCCGGC 1 cut(s) 290
KroNI GCCGGC 1 cut(s) 292
Kzo9I GATC 3 cut(s) 24, 197, 214
Lsp1109I GCAGC 1 cut(s) 884
LweI GCATC 1 cut(s) 948
MaeI CTAG 2 cut(s) 306, 659
MaeIII GTNAC 1 cut(s) 554
MalI GATC 3 cut(s) 26, 199, 216
MboI GATC 3 cut(s) 24, 197, 214
MboII GAAGA 7 cut(s) 19, 67, 217, 415, 799, 985, 1010
MfeI CAATTG 2 cut(s) 434, 561
MflI RGATCY 1 cut(s) 197
MhlI GDGCHC 1 cut(s) 993
Mly113I GGCGCC 1 cut(s) 177
MlyI GAGTC 2 cut(s) 286, 551
MroNI GCCGGC 1 cut(s) 290
MseI TTAA 2 cut(s) 339, 507
MspI CCGG 7 cut(s) 68, 166, 291, 327, 380, 473, 654
MspR9I CCNGG 5 cut(s) 167, 327, 328, 655, 914
MunI CAATTG 2 cut(s) 434, 561
Mva1269I GAATGC 1 cut(s) 1007
MvaI CCWGG 1 cut(s) 914
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 2 cut(s) 263, 632
NaeI GCCGGC 1 cut(s) 292
NarI GGCGCC 1 cut(s) 177
NciI CCSGG 4 cut(s) 167, 327, 328, 655
NdeI CATATG 1 cut(s) 706
NdeII GATC 3 cut(s) 24, 197, 214
NgoMIV GCCGGC 1 cut(s) 290
NlaIII CATG 3 cut(s) 462, 778, 829
NlaIV GGNNCC 1 cut(s) 178
NmeAIII GCCGAG 1 cut(s) 283
NmuCI GTSAC 1 cut(s) 554
NspV TTCGAA 1 cut(s) 229
PctI GAATGC 1 cut(s) 1007
PdiI GCCGGC 1 cut(s) 292
PfeI GAWTC 1 cut(s) 100
PkrI GCNGC 3 cut(s) 152, 579, 874
Ple19I CGATCG 1 cut(s) 217
PleI GAGTC 2 cut(s) 285, 551
PluTI GGCGCC 1 cut(s) 180
PpsI GAGTC 2 cut(s) 285, 551
Psp124BI GAGCTC 1 cut(s) 993
Psp6I CCWGG 1 cut(s) 912
PspFI CCCAGC 1 cut(s) 899
PspGI CCWGG 1 cut(s) 912
PspN4I GGNNCC 1 cut(s) 178
PspPI GGNCC 5 cut(s) 38, 288, 415, 651, 800
PstI CTGCAG 2 cut(s) 628, 945
PsuI RGATCY 1 cut(s) 197
PvuI CGATCG 1 cut(s) 217
RsaI GTAC 4 cut(s) 491, 719, 812, 824
RsaNI GTAC 4 cut(s) 490, 718, 811, 823
SacI GAGCTC 1 cut(s) 993
SaqAI TTAA 2 cut(s) 339, 507
SatI GCNGC 3 cut(s) 151, 578, 873
Sau3AI GATC 3 cut(s) 24, 197, 214
Sau96I GGNCC 5 cut(s) 38, 288, 415, 651, 800
SchI GAGTC 2 cut(s) 286, 551
ScrFI CCNGG 5 cut(s) 167, 327, 328, 655, 914
SduI GDGCHC 1 cut(s) 993
SetI ASST 7 cut(s) 91, 228, 376, 570, 653, 993, 1034
SfaNI GCATC 1 cut(s) 948
SfcI CTRYAG 2 cut(s) 624, 941
SfoI GGCGCC 1 cut(s) 178
SfuI TTCGAA 1 cut(s) 229
SinI GGWCC 3 cut(s) 415, 651, 800
SmaI CCCGGG 1 cut(s) 328
SmlI CTYRAG 2 cut(s) 369, 962
SmoI CTYRAG 2 cut(s) 369, 962
SsiI CCGC 8 cut(s) 60, 82, 115, 151, 547, 577, 855, 1005
SspDI GGCGCC 1 cut(s) 176
SspMI CTAG 2 cut(s) 306, 659
SstI GAGCTC 1 cut(s) 993
StyD4I CCNGG 5 cut(s) 165, 325, 326, 653, 912
StyI CCWWGG 1 cut(s) 880
TaaI ACNGT 4 cut(s) 334, 533, 849, 893
TaqI TCGA 3 cut(s) 222, 229, 312
TaqII GACCGA 1 cut(s) 817
TatI WGTACW 1 cut(s) 822
TauI GCSGC 2 cut(s) 153, 580
TfiI GAWTC 1 cut(s) 100
Tru1I TTAA 2 cut(s) 339, 507
Tru9I TTAA 2 cut(s) 339, 507
TscAI CASTG 5 cut(s) 339, 520, 616, 742, 945
TseFI GTSAC 1 cut(s) 554
TseI GCWGC 1 cut(s) 872
Tsp45I GTSAC 1 cut(s) 554
TspDTI ATGAA 3 cut(s) 416, 763, 845
TspMI CCCGGG 1 cut(s) 326
TspRI CASTG 5 cut(s) 339, 520, 616, 742, 945
VpaK11BI GGWCC 3 cut(s) 415, 651, 800
XapI RAATTY 3 cut(s) 320, 348, 466
XmaI CCCGGG 1 cut(s) 326
XmiI GTMKAC 1 cut(s) 528
XspI CTAG 2 cut(s) 306, 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.