MD03G1041900.v1.1

CCR4-associated factor 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000757
Physical Location & Seq
Forward (+)
3313975 .. 3314448
474 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1041900.v1.1.491

Sequence Viewer

Length: 474 bp
ATGGAGAACAAGGAAAAGGGTATTCGTTCTTCCGACTTCGCCTTGCTGTGGCGTAAATTTTTGCTGTTCTCCAAGAAATCCCGGTTGACATGGGTGACTTTCCATAGCGCTTACGATTTCGGGTACCTGATCAAGATGCTGAATCACAATCGTTTGCCGCAAGACATAAACACTTTCACGGACATGATGGATATATACTTTGGGAAAAATGTGTATGACATCAAAAGCATGATTAGGGTTTCTCGAGAGATTTATGGAGGATTAGAGAGAGTGGCTAACAAGCTTGGGGTTGATCGTGTGGCTGGGAAGAGTCATCAGGCTGCTTATGATAGTTTGCTGACTTGGCAAACGTTTCGGAAGCTTACAGATGTGTATCACAGAAGCATGCTAGGGGAAGATGGCTGCAGTTTAGATTTGAGAGATTTGGATTGGTCTCAAAGGGTTTTGTATGGTTTGGAAGTTGAGGCTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1 RVT_3

Protein Analysis

158

Amino Acids

18.45

Weight (kDa)

8.94

Isoelectric Point (pI)

31.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 123
AccB1I GGYRCC 1 cut(s) 123
AciI CCGC 1 cut(s) 158
AclI AACGTT 1 cut(s) 350
AcsI RAATTY 1 cut(s) 56
AfaI GTAC 1 cut(s) 125
AfeI AGCGCT 1 cut(s) 109
AfiI CCNNNNNNNGG 1 cut(s) 48
AluBI AGCT 2 cut(s) 283, 361
AluI AGCT 2 cut(s) 283, 361
Alw26I GTCTC 1 cut(s) 438
Ama87I CYCGRG 1 cut(s) 243
Aor51HI AGCGCT 1 cut(s) 109
ApeKI GCWGC 2 cut(s) 320, 402
ApoI RAATTY 1 cut(s) 56
Asp718I GGTACC 1 cut(s) 123
AspLEI GCGC 1 cut(s) 110
AsuC2I CCSGG 1 cut(s) 82
AsuHPI GGTGA 1 cut(s) 106
AvaI CYCGRG 1 cut(s) 243
BanI GGYRCC 1 cut(s) 123
BarI GAAGNNNNNNTAC 2 cut(s) 13, 45
BbvI GCAGC 2 cut(s) 307, 389
BccI CCATC 2 cut(s) 181, 392
BcgI CGANNNNNNTGC 2 cut(s) 335, 369
BclI TGATCA 1 cut(s) 129
BcnI CCSGG 1 cut(s) 82
BcoDI GTCTC 1 cut(s) 438
BfaI CTAG 1 cut(s) 389
BfmI CTRYAG 1 cut(s) 403
BfoI RGCGCY 1 cut(s) 111
BisI GCNGC 3 cut(s) 158, 321, 403
BlsI GCNGC 3 cut(s) 159, 322, 404
Bme1390I CCNGG 1 cut(s) 82
BmeT110I CYCGRG 1 cut(s) 243
BmiI GGNNCC 1 cut(s) 125
BmrFI CCNGG 1 cut(s) 82
BmsI GCATC 1 cut(s) 126
BpuMI CCSGG 1 cut(s) 82
BsaBI GATNNNNATC 1 cut(s) 372
BsaI GGTCTC 1 cut(s) 438
Bsc4I CCNNNNNNNGG 1 cut(s) 48
Bse8I GATNNNNATC 1 cut(s) 372
BseJI GATNNNNATC 1 cut(s) 372
BseLI CCNNNNNNNGG 1 cut(s) 48
BseXI GCAGC 2 cut(s) 307, 389
BseYI CCCAGC 1 cut(s) 302
BshNI GGYRCC 1 cut(s) 123
BsiHKCI CYCGRG 1 cut(s) 243
BsiSI CCGG 1 cut(s) 82
BslI CCNNNNNNNGG 1 cut(s) 48
BsmAI GTCTC 1 cut(s) 438
Bso31I GGTCTC 1 cut(s) 438
BsoBI CYCGRG 1 cut(s) 243
Bsp143I GATC 2 cut(s) 129, 292
BspACI CCGC 1 cut(s) 158
BspLI GGNNCC 1 cut(s) 125
BspMAI CTGCAG 1 cut(s) 407
BspT107I GGYRCC 1 cut(s) 123
BspTNI GGTCTC 1 cut(s) 438
BssMI GATC 2 cut(s) 129, 292
Bst6I CTCTTC 1 cut(s) 302
BstC8I GCNNGC 1 cut(s) 386
BstH2I RGCGCY 1 cut(s) 111
BstHHI GCGC 1 cut(s) 110
BstKTI GATC 2 cut(s) 132, 295
BstMAI GTCTC 1 cut(s) 438
BstMBI GATC 2 cut(s) 129, 292
BstMWI GCNNNNNNNGC 1 cut(s) 343
BstNSI RCATGY 1 cut(s) 388
BstSCI CCNGG 1 cut(s) 80
BstSFI CTRYAG 1 cut(s) 403
BstV1I GCAGC 2 cut(s) 307, 389
Cac8I GCNNGC 1 cut(s) 386
CfoI GCGC 1 cut(s) 110
Csp6I GTAC 1 cut(s) 124
CviAII CATG 4 cut(s) 90, 184, 229, 385
CviJI RGCY 7 cut(s) 275, 283, 302, 320, 361, 402, 467
CviKI_1 RGCY 7 cut(s) 275, 283, 302, 320, 361, 402, 467
CviQI GTAC 1 cut(s) 124
DpnI GATC 2 cut(s) 131, 294
DpnII GATC 2 cut(s) 129, 292
Eam1104I CTCTTC 1 cut(s) 302
EarI CTCTTC 1 cut(s) 302
Eco31I GGTCTC 1 cut(s) 438
Eco47III AGCGCT 1 cut(s) 109
Eco88I CYCGRG 1 cut(s) 243
FaeI CATG 4 cut(s) 93, 187, 232, 388
FatI CATG 4 cut(s) 89, 183, 228, 384
FbaI TGATCA 1 cut(s) 129
Fnu4HI GCNGC 3 cut(s) 158, 321, 403
Fsp4HI GCNGC 3 cut(s) 158, 321, 403
FspBI CTAG 1 cut(s) 389
GlaI GCGC 1 cut(s) 109
GluI GCNGC 3 cut(s) 158, 321, 403
GsaI CCCAGC 1 cut(s) 306
HaeII RGCGCY 1 cut(s) 111
HapII CCGG 1 cut(s) 82
HhaI GCGC 1 cut(s) 110
Hin1II CATG 4 cut(s) 93, 187, 232, 388
Hin6I GCGC 1 cut(s) 108
HinP1I GCGC 1 cut(s) 108
HincII GTYRAC 1 cut(s) 87
HindII GTYRAC 1 cut(s) 87
HindIII AAGCTT 2 cut(s) 281, 359
HinfI GANTC 2 cut(s) 142, 310
HpaII CCGG 1 cut(s) 82
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 1 cut(s) 87
Hpy188I TCNGA 2 cut(s) 34, 357
Hpy188III TCNNGA 3 cut(s) 133, 243, 245
Hpy8I GTNNAC 1 cut(s) 87
HpyCH4IV ACGT 1 cut(s) 350
HpyCH4V TGCA 1 cut(s) 405
HpyF10VI GCNNNNNNNGC 1 cut(s) 343
HpySE526I ACGT 1 cut(s) 350
Hsp92II CATG 4 cut(s) 93, 187, 232, 388
HspAI GCGC 1 cut(s) 108
KpnI GGTACC 1 cut(s) 127
Ksp22I TGATCA 1 cut(s) 129
Kzo9I GATC 2 cut(s) 129, 292
LpnPI CCDG 4 cut(s) 95, 140, 288, 302
Lsp1109I GCAGC 2 cut(s) 307, 389
LweI GCATC 1 cut(s) 126
MaeI CTAG 1 cut(s) 389
MaeII ACGT 1 cut(s) 350
MaeIII GTNAC 1 cut(s) 94
MalI GATC 2 cut(s) 131, 294
MboI GATC 2 cut(s) 129, 292
MboII GAAGA 3 cut(s) 21, 319, 407
MluCI AATT 1 cut(s) 56
MlyI GAGTC 1 cut(s) 319
MmeI TCCRAC 1 cut(s) 57
MnlI CCTC 2 cut(s) 251, 457
MslI CAYNNNNRTG 1 cut(s) 182
MspI CCGG 1 cut(s) 82
MspR9I CCNGG 1 cut(s) 82
MwoI GCNNNNNNNGC 1 cut(s) 343
NciI CCSGG 1 cut(s) 82
NdeII GATC 2 cut(s) 129, 292
NlaIII CATG 4 cut(s) 93, 187, 232, 388
NlaIV GGNNCC 1 cut(s) 125
NmuCI GTSAC 1 cut(s) 94
NspI RCATGY 1 cut(s) 388
PaeI GCATGC 1 cut(s) 388
PaeR7I CTCGAG 1 cut(s) 243
PfeI GAWTC 1 cut(s) 142
PkrI GCNGC 3 cut(s) 159, 322, 404
PleI GAGTC 1 cut(s) 318
PpsI GAGTC 1 cut(s) 318
Psp1406I AACGTT 1 cut(s) 350
PspFI CCCAGC 1 cut(s) 302
PspN4I GGNNCC 1 cut(s) 125
PstI CTGCAG 1 cut(s) 407
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
RseI CAYNNNNRTG 1 cut(s) 182
SatI GCNGC 3 cut(s) 158, 321, 403
Sau3AI GATC 2 cut(s) 129, 292
SchI GAGTC 1 cut(s) 319
ScrFI CCNGG 1 cut(s) 82
SetI ASST 4 cut(s) 129, 285, 353, 363
SfaNI GCATC 1 cut(s) 126
SfcI CTRYAG 1 cut(s) 403
Sfr274I CTCGAG 1 cut(s) 243
SlaI CTCGAG 1 cut(s) 243
SmiMI CAYNNNNRTG 1 cut(s) 182
SmlI CTYRAG 1 cut(s) 243
SmoI CTYRAG 1 cut(s) 243
SphI GCATGC 1 cut(s) 388
Sse9I AATT 1 cut(s) 56
SsiI CCGC 1 cut(s) 158
SspMI CTAG 1 cut(s) 389
StyD4I CCNGG 1 cut(s) 80
TaiI ACGT 1 cut(s) 353
TaqI TCGA 1 cut(s) 244
TasI AATT 1 cut(s) 56
TauI GCSGC 1 cut(s) 160
TfiI GAWTC 1 cut(s) 142
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 2 cut(s) 320, 402
Tsp45I GTSAC 1 cut(s) 94
TspGWI ACGGA 1 cut(s) 194
XapI RAATTY 1 cut(s) 56
XceI RCATGY 1 cut(s) 388
XhoI CTCGAG 1 cut(s) 243
XspI CTAG 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.