FvH4_6g29280

CCR4-associated factor 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000757
Physical Location & Seq
Reverse (-)
22585849 .. 22588579
2731 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g29280.t1

Sequence Viewer

Length: 1200 bp
ATGATGATCGTGCAGAGAGGGATTCCTCTCTTCCCAATCTCTCCTGCTCCTCAATTCCGACCTACCCCTCCAATTGCTCTACTCCACCACCGCCCTTATCCTCCTCCGCTCTCCCCCGTGAAGATCTCGGATCGGGTCGAGGTTCGGAAAGTGTGTCGCTACAATCTCAAAGCCGAGCTCGCCGTTATTCAGACTCTGATCAGGACCGGCTTTCGAGTGGCTAGCTTCGACACCGAGTTCCCCGGAACAGTGGTGCCCTCTAAGGTTCCAAGGCATTTGATTTCTCAGGCGGGTCCTGCTGATGTTTACGAGATGATGAAAGCTAATGTGGACAAGACCCACATTATTCAATTGGGGCTTTCACTTTGTGACCCGTGTGGGAACTTGCCTGTTTACGATGGCGCTTACTGCTGCTGGGAGTTCAACTTTAGTGATTTCGATGTGGAGTGTGATGATCATATGAGGAATAGTGATTCGATTGATCTGTTGGAGAGGCAAGGAATTGACTTTTCTGAGAATAGAGCTACTGGTATTTGTTCGGCTGATTTTGCTGAGGAGATTGTGAGATCTGGGCTTGCGCAGTTGCTTCCCTGCTTGACTTGGGCAACATTTCAATGTGCCTATGATTTTGGGTACCTGGTGAAGGTTTTCAGTGGGAAGAAGGAATTGCCTGAGGATGTGAATGGGTTTATGTCTCATGTTAAGGCTTACTTTGGACACAAGGTGTATGACATCAAGTACATGATGAGATACTGTGATAGGTTGTTTGGAGGTTTGGACAGAGTGGCAGAAACTCTTGGTGTCTGTCGTGTCGCTGGGAGTAGCCACCAGGCTGGTTCAGATAGTCTTTTGACTCTGCAGACATTTATTAAGATTCTCAACATGGAGAAGAGCAAAGGGAAAGAGCTCTCTGAGATCAAAAGATACCCATATGCTCATGTCTATGAGTATTCCAAATTGTTCCTACTGCTCTTCTCCTATGAGCTTCATCGCCAACTTGGCTTGTTGGACATATCTCATCACATCTTTGTCAATGCTGTGGATCCTGATGTTGTTGAAACCAAAATGATGCGAGAATTTCCTCCATGCCTATCCAGTTTGGCTTTTAAAATTTTGAGAAGTTGCCTCTTGCAGTCACCTGAAGTTGGAATTGGCTCCATTCTTGATGCAGTAGTTGCTCCACCAGTAAGCTTATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1 RVT_3

Protein Analysis

400

Amino Acids

44.9

Weight (kDa)

6.32

Isoelectric Point (pI)

41.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 52 - 178 4.8e-08 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 579
Acc65I GGTACC 1 cut(s) 633
AccB1I GGYRCC 2 cut(s) 253, 633
AccBSI CCGCTC 1 cut(s) 109
AciI CCGC 3 cut(s) 91, 107, 290
AclWI GGATC 3 cut(s) 138, 1037, 1050
AcsI RAATTY 2 cut(s) 1076, 1110
AcuI CTGAAG 1 cut(s) 1161
AdeI CACNNNGTG 2 cut(s) 368, 724
AfaI GTAC 2 cut(s) 635, 740
AfiI CCNNNNNNNGG 2 cut(s) 643, 1145
AgsI TTSAA 4 cut(s) 350, 424, 614, 1058
AjnI CCWGG 2 cut(s) 636, 828
AjuI GAANNNNNNNTTGG 2 cut(s) 1134, 1166
AluBI AGCT 7 cut(s) 178, 225, 323, 524, 907, 985, 1191
AluI AGCT 7 cut(s) 178, 225, 323, 524, 907, 985, 1191
Alw21I GWGCWC 2 cut(s) 180, 909
Alw26I GTCTC 1 cut(s) 699
AlwI GGATC 3 cut(s) 138, 1037, 1050
AlwNI CAGNNNCTG 1 cut(s) 196
ApeKI GCWGC 1 cut(s) 411
ApoI RAATTY 2 cut(s) 1076, 1110
Asp700I GAANNNNTTC 1 cut(s) 647
Asp718I GGTACC 1 cut(s) 633
AspLEI GCGC 2 cut(s) 404, 580
AspS9I GGNCC 2 cut(s) 204, 293
AsuC2I CCSGG 1 cut(s) 243
AsuHPI GGTGA 2 cut(s) 652, 1128
AsuNHI GCTAGC 1 cut(s) 221
AvaII GGWCC 2 cut(s) 204, 293
AxyI CCTNAGG 1 cut(s) 672
BaeGI GKGCMC 1 cut(s) 258
BamHI GGATCC 1 cut(s) 1042
BanI GGYRCC 2 cut(s) 253, 633
BanII GRGCYC 2 cut(s) 180, 909
Bbv12I GWGCWC 2 cut(s) 180, 909
BbvCI CCTCAGC 1 cut(s) 552
BbvI GCAGC 1 cut(s) 398
BccI CCATC 1 cut(s) 392
BceAI ACGGC 1 cut(s) 167
BciT130I CCWGG 2 cut(s) 638, 830
BclI TGATCA 2 cut(s) 198, 454
BcnI CCSGG 1 cut(s) 243
BcoDI GTCTC 1 cut(s) 699
BfaI CTAG 1 cut(s) 222
BfmI CTRYAG 1 cut(s) 857
BfoI RGCGCY 1 cut(s) 405
BglI GCCNNNNNGGC 1 cut(s) 999
BglII AGATCT 2 cut(s) 123, 566
BisI GCNGC 1 cut(s) 412
BlsI GCNGC 1 cut(s) 413
Bme1390I CCNGG 3 cut(s) 243, 638, 830
Bme18I GGWCC 2 cut(s) 204, 293
BmgT120I GGNCC 2 cut(s) 204, 293
BmiI GGNNCC 6 cut(s) 255, 267, 294, 635, 1044, 1156
BmrFI CCNGG 3 cut(s) 243, 638, 830
BmsI GCATC 2 cut(s) 1059, 1156
BmtI GCTAGC 1 cut(s) 225
Bpu10I CCTNAGC 1 cut(s) 552
BpuMI CCSGG 1 cut(s) 243
BsaJI CCNNGG 2 cut(s) 241, 269
Bsc4I CCNNNNNNNGG 2 cut(s) 643, 1145
Bse118I RCCGGY 1 cut(s) 206
Bse1I ACTGG 3 cut(s) 532, 1095, 1184
Bse21I CCTNAGG 1 cut(s) 672
BseBI CCWGG 2 cut(s) 638, 830
BseDI CCNNGG 2 cut(s) 241, 269
BseGI GGATG 1 cut(s) 682
BseLI CCNNNNNNNGG 2 cut(s) 643, 1145
BseMII CTCAG 5 cut(s) 299, 504, 543, 663, 903
BseNI ACTGG 3 cut(s) 532, 1095, 1184
BseRI GAGGAG 3 cut(s) 39, 93, 569
BseSI GKGCMC 1 cut(s) 258
BseXI GCAGC 1 cut(s) 398
BseYI CCCAGC 2 cut(s) 414, 815
BsgI GTGCAG 1 cut(s) 32
BshNI GGYRCC 2 cut(s) 253, 633
BsiHKAI GWGCWC 2 cut(s) 180, 909
BsiSI CCGG 2 cut(s) 207, 243
BslI CCNNNNNNNGG 2 cut(s) 643, 1145
BsmAI GTCTC 1 cut(s) 699
Bsp1286I GDGCHC 3 cut(s) 180, 258, 909
Bsp143I GATC 9 cut(s) 6, 123, 130, 198, 454, 481, 566, 915, 1042
BspACI CCGC 3 cut(s) 91, 107, 290
BspCNI CTCAG 5 cut(s) 298, 505, 544, 664, 904
BspLI GGNNCC 6 cut(s) 255, 267, 294, 635, 1044, 1156
BspMAI CTGCAG 1 cut(s) 861
BspOI GCTAGC 1 cut(s) 225
BspPI GGATC 3 cut(s) 138, 1037, 1050
BspQI GCTCTTC 2 cut(s) 884, 977
BspT107I GGYRCC 2 cut(s) 253, 633
BsrBI CCGCTC 1 cut(s) 109
BsrFI RCCGGY 1 cut(s) 206
BsrI ACTGG 3 cut(s) 532, 1095, 1184
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 2 cut(s) 241, 269
BssMI GATC 9 cut(s) 6, 123, 130, 198, 454, 481, 566, 915, 1042
BssT1I CCWWGG 1 cut(s) 269
Bst2UI CCWGG 2 cut(s) 638, 830
Bst4CI ACNGT 2 cut(s) 250, 755
Bst6I CTCTTC 3 cut(s) 35, 884, 977
BstAPI GCANNNNNTGC 1 cut(s) 1175
BstC8I GCNNGC 3 cut(s) 180, 223, 576
BstDEI CTNAG 6 cut(s) 261, 285, 513, 552, 672, 912
BstENI CCTNNNNNAGG 1 cut(s) 641
BstF5I GGATG 1 cut(s) 682
BstH2I RGCGCY 1 cut(s) 405
BstHHI GCGC 2 cut(s) 404, 580
BstKTI GATC 9 cut(s) 9, 126, 133, 201, 457, 484, 569, 918, 1045
BstMAI GTCTC 1 cut(s) 699
BstMBI GATC 9 cut(s) 6, 123, 130, 198, 454, 481, 566, 915, 1042
BstMWI GCNNNNNNNGC 6 cut(s) 179, 296, 408, 548, 999, 1175
BstNI CCWGG 2 cut(s) 638, 830
BstSCI CCNGG 3 cut(s) 241, 636, 828
BstSFI CTRYAG 1 cut(s) 857
BstSLI GKGCMC 1 cut(s) 258
BstV1I GCAGC 1 cut(s) 398
BstX2I RGATCY 3 cut(s) 123, 566, 1042
BstXI CCANNNNNNTGG 1 cut(s) 833
BstYI RGATCY 3 cut(s) 123, 566, 1042
Bsu36I CCTNAGG 1 cut(s) 672
BtgZI GCGATG 1 cut(s) 974
BtsCI GGATG 1 cut(s) 682
BtsIMutI CAGTG 2 cut(s) 255, 658
Cac8I GCNNGC 3 cut(s) 180, 223, 576
CaiI CAGNNNCTG 1 cut(s) 196
CfoI GCGC 2 cut(s) 404, 580
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 2 cut(s) 204, 293
CsiI ACCWGGT 1 cut(s) 636
Csp6I GTAC 2 cut(s) 634, 739
CviAII CATG 5 cut(s) 698, 742, 883, 938, 1086
CviQI GTAC 2 cut(s) 634, 739
DdeI CTNAG 6 cut(s) 261, 285, 513, 552, 672, 912
DpnI GATC 9 cut(s) 8, 125, 132, 200, 456, 483, 568, 917, 1044
DpnII GATC 9 cut(s) 6, 123, 130, 198, 454, 481, 566, 915, 1042
DraI TTTAAA 1 cut(s) 1108
DraIII CACNNNGTG 2 cut(s) 368, 724
Eam1104I CTCTTC 3 cut(s) 35, 884, 977
EarI CTCTTC 3 cut(s) 35, 884, 977
Ecl136II GAGCTC 2 cut(s) 178, 907
Eco130I CCWWGG 1 cut(s) 269
Eco24I GRGCYC 2 cut(s) 180, 909
Eco47I GGWCC 2 cut(s) 204, 293
Eco53kI GAGCTC 2 cut(s) 178, 907
Eco57I CTGAAG 1 cut(s) 1161
Eco81I CCTNAGG 1 cut(s) 672
EcoICRI GAGCTC 2 cut(s) 178, 907
EcoNI CCTNNNNNAGG 1 cut(s) 641
EcoO109I RGGNCCY 1 cut(s) 293
EcoRII CCWGG 2 cut(s) 636, 828
EcoT14I CCWWGG 1 cut(s) 269
EcoT38I GRGCYC 2 cut(s) 180, 909
ErhI CCWWGG 1 cut(s) 269
FaeI CATG 5 cut(s) 701, 745, 886, 941, 1089
FalI AAGNNNNNCTT 2 cut(s) 695, 727
FatI CATG 5 cut(s) 697, 741, 882, 937, 1085
FauI CCCGC 1 cut(s) 283
FauNDI CATATG 2 cut(s) 459, 931
FbaI TGATCA 2 cut(s) 198, 454
Fnu4HI GCNGC 1 cut(s) 412
FokI GGATG 1 cut(s) 689
FriOI GRGCYC 2 cut(s) 180, 909
Fsp4HI GCNGC 1 cut(s) 412
FspBI CTAG 1 cut(s) 222
FspI TGCGCA 1 cut(s) 579
GlaI GCGC 2 cut(s) 403, 579
GluI GCNGC 1 cut(s) 412
GsaI CCCAGC 2 cut(s) 418, 819
HaeII RGCGCY 1 cut(s) 405
HapII CCGG 2 cut(s) 207, 243
HhaI GCGC 2 cut(s) 404, 580
Hin1II CATG 5 cut(s) 701, 745, 886, 941, 1089
Hin6I GCGC 2 cut(s) 402, 578
HinP1I GCGC 2 cut(s) 402, 578
HindIII AAGCTT 1 cut(s) 1189
HinfI GANTC 5 cut(s) 22, 193, 473, 853, 874
HpaII CCGG 2 cut(s) 207, 243
HphI GGTGA 2 cut(s) 652, 1128
Hpy166II GTNNAC 3 cut(s) 307, 331, 394
Hpy188I TCNGA 8 cut(s) 59, 130, 147, 192, 198, 514, 841, 913
Hpy188III TCNNGA 3 cut(s) 202, 1046, 1163
Hpy8I GTNNAC 3 cut(s) 307, 331, 394
HpyAV CCTTC 2 cut(s) 637, 655
HpyCH4III ACNGT 2 cut(s) 250, 755
HpyCH4V TGCA 4 cut(s) 13, 859, 1132, 1169
HpyF10VI GCNNNNNNNGC 6 cut(s) 179, 296, 408, 548, 999, 1175
HpyF3I CTNAG 6 cut(s) 261, 285, 513, 552, 672, 912
Hsp92II CATG 5 cut(s) 701, 745, 886, 941, 1089
HspAI GCGC 2 cut(s) 402, 578
KpnI GGTACC 1 cut(s) 637
Ksp22I TGATCA 2 cut(s) 198, 454
Kzo9I GATC 9 cut(s) 6, 123, 130, 198, 454, 481, 566, 915, 1042
LguI GCTCTTC 2 cut(s) 884, 977
LmnI GCTCC 3 cut(s) 52, 1160, 1183
Lsp1109I GCAGC 1 cut(s) 398
LweI GCATC 2 cut(s) 1059, 1156
MabI ACCWGGT 1 cut(s) 636
MaeI CTAG 1 cut(s) 222
MaeIII GTNAC 2 cut(s) 368, 1134
MalI GATC 9 cut(s) 8, 125, 132, 200, 456, 483, 568, 917, 1044
MbiI CCGCTC 1 cut(s) 109
MboI GATC 9 cut(s) 6, 123, 130, 198, 454, 481, 566, 915, 1042
MboII GAAGA 5 cut(s) 22, 133, 670, 901, 964
MfeI CAATTG 2 cut(s) 72, 350
MflI RGATCY 3 cut(s) 123, 566, 1042
MhlI GDGCHC 3 cut(s) 180, 258, 909
MluCI AATT 9 cut(s) 53, 72, 350, 501, 665, 956, 1076, 1110, 1149
MlyI GAGTC 2 cut(s) 187, 847
MmeI TCCRAC 4 cut(s) 82, 468, 987, 1126
MroXI GAANNNNTTC 1 cut(s) 647
MseI TTAA 3 cut(s) 702, 870, 1107
MslI CAYNNNNRTG 2 cut(s) 613, 942
MspI CCGG 2 cut(s) 207, 243
MspR9I CCNGG 3 cut(s) 243, 638, 830
MunI CAATTG 2 cut(s) 72, 350
MvaI CCWGG 2 cut(s) 638, 830
MwoI GCNNNNNNNGC 6 cut(s) 179, 296, 408, 548, 999, 1175
NciI CCSGG 1 cut(s) 243
NdeI CATATG 2 cut(s) 459, 931
NdeII GATC 9 cut(s) 6, 123, 130, 198, 454, 481, 566, 915, 1042
NheI GCTAGC 1 cut(s) 221
NlaIII CATG 5 cut(s) 701, 745, 886, 941, 1089
NlaIV GGNNCC 6 cut(s) 255, 267, 294, 635, 1044, 1156
NmeAIII GCCGAG 1 cut(s) 199
NmuCI GTSAC 2 cut(s) 368, 1134
NsbI TGCGCA 1 cut(s) 579
PciSI GCTCTTC 2 cut(s) 884, 977
PdmI GAANNNNTTC 1 cut(s) 647
PfeI GAWTC 3 cut(s) 22, 473, 874
PkrI GCNGC 1 cut(s) 413
PleI GAGTC 2 cut(s) 187, 847
PpsI GAGTC 2 cut(s) 187, 847
PpuMI RGGWCCY 1 cut(s) 293
Psp124BI GAGCTC 2 cut(s) 180, 909
Psp5II RGGWCCY 1 cut(s) 293
Psp6I CCWGG 2 cut(s) 636, 828
PspFI CCCAGC 2 cut(s) 414, 815
PspGI CCWGG 2 cut(s) 636, 828
PspN4I GGNNCC 6 cut(s) 255, 267, 294, 635, 1044, 1156
PspPI GGNCC 2 cut(s) 204, 293
PspPPI RGGWCCY 1 cut(s) 293
PstI CTGCAG 1 cut(s) 861
PstNI CAGNNNCTG 1 cut(s) 196
PsuI RGATCY 3 cut(s) 123, 566, 1042
RsaI GTAC 2 cut(s) 635, 740
RsaNI GTAC 2 cut(s) 634, 739
RseI CAYNNNNRTG 2 cut(s) 613, 942
SacI GAGCTC 2 cut(s) 180, 909
SapI GCTCTTC 2 cut(s) 884, 977
SaqAI TTAA 3 cut(s) 702, 870, 1107
SatI GCNGC 1 cut(s) 412
Sau3AI GATC 9 cut(s) 6, 123, 130, 198, 454, 481, 566, 915, 1042
Sau96I GGNCC 2 cut(s) 204, 293
SchI GAGTC 2 cut(s) 187, 847
ScrFI CCNGG 3 cut(s) 243, 638, 830
SduI GDGCHC 3 cut(s) 180, 258, 909
SexAI ACCWGGT 1 cut(s) 636
SfaNI GCATC 2 cut(s) 1059, 1156
SfcI CTRYAG 1 cut(s) 857
SinI GGWCC 2 cut(s) 204, 293
SmiMI CAYNNNNRTG 2 cut(s) 613, 942
Sse9I AATT 9 cut(s) 53, 72, 350, 501, 665, 956, 1076, 1110, 1149
SsiI CCGC 3 cut(s) 91, 107, 290
SspMI CTAG 1 cut(s) 222
SstI GAGCTC 2 cut(s) 180, 909
StyD4I CCNGG 3 cut(s) 241, 636, 828
StyI CCWWGG 1 cut(s) 269
TaaI ACNGT 2 cut(s) 250, 755
TaqI TCGA 5 cut(s) 138, 214, 228, 438, 476
TasI AATT 9 cut(s) 53, 72, 350, 501, 665, 956, 1076, 1110, 1149
TatI WGTACW 1 cut(s) 738
TfiI GAWTC 3 cut(s) 22, 473, 874
Tru1I TTAA 3 cut(s) 702, 870, 1107
Tru9I TTAA 3 cut(s) 702, 870, 1107
TscAI CASTG 2 cut(s) 255, 658
TseFI GTSAC 2 cut(s) 368, 1134
TseI GCWGC 1 cut(s) 411
Tsp45I GTSAC 2 cut(s) 368, 1134
TspDTI ATGAA 2 cut(s) 332, 977
TspRI CASTG 2 cut(s) 255, 658
VpaK11BI GGWCC 2 cut(s) 204, 293
XagI CCTNNNNNAGG 1 cut(s) 641
XapI RAATTY 2 cut(s) 1076, 1110
XmnI GAANNNNTTC 1 cut(s) 647
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.