RchiOBHm_Chr2g0167861

CCR4-associated factor 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000757
Physical Location & Seq
Reverse (-)
82182654 .. 82183478
825 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53562

Sequence Viewer

Length: 825 bp
ATGTCTGCAGTTTCCCTCACCTCCTGTGCCGGTCATAAATCCTTACTTGGGTACAATGGAGTACTGCTGCCCATGCATGATCCCGACCCTCTTCCTATACCTGGCGTTATATATCCGGTTTGGAAGAGCAATTTTGTGCCTGCGTTTTCGCAGATTTGGAAACTAATTAGCCAAGGTGGCCGCTGGTTCGCTTCTATAGACACAGAATTCCCTGGCACAATTTATGATTGTGACAAAGGCCGTCTCCATGACCCTCTGTACAAGTATATGATGATGAAGAAGAACGTGAACGACACCAACATAATCCAGTTGGGTCTCACACTCGCAGACCTTAACCAAAACATCTACACCTGGGAGTTCAATTTCAGCGACTTTGATATTGACCGTGAAGATCACAAGCATATCAAGCGCTCAATTGAGTTACTTAAGCGTCACGGAATCGATTTCTGGAGAAACAAGGAGGATGGTATACCTTCGCATGAGTTCGCGAGAATGTGTCGGGAAGTTGGGCTGTTAGAAAACAGATTGGTAGAGTTGACATGGGTGGGTTTCCAGATCTCATCTGATTTGGGCTACTTCACCAAGATCCTCACTGGACAAAAGTTGCCAGACGATTTTGAGGAGTACAAGAAGGCTGAAAGGATCTACTTTGGGGATAGGGTTTGTGATGTCAAGGACATGATGAACTCGTTTCCGACACTGAAAGGCGGTTTGACCAAAGTGGCGGAGAAACTGGGGATTGCACGTGTGGCCGGCAAGAGTCACCAGGCCGGCTCTGATAGTCTGTTGACATATCTAAGCTTCATCAAGCTGAGCTCAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1

Protein Analysis

274

Amino Acids

31.3

Weight (kDa)

6.71

Isoelectric Point (pI)

34.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 40 - 158 6.2e-11 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 469
AccII CGCG 1 cut(s) 488
AciI CCGC 3 cut(s) 181, 708, 725
AclWI GGATC 3 cut(s) 74, 580, 650
AcoI YGGCCR 2 cut(s) 178, 750
AcsI RAATTY 1 cut(s) 206
AcvI CACGTG 1 cut(s) 746
AfaI GTAC 4 cut(s) 53, 63, 260, 626
AfeI AGCGCT 1 cut(s) 410
AfiI CCNNNNNNNGG 2 cut(s) 48, 101
AflII CTTAAG 1 cut(s) 425
AflIII ACRYGT 1 cut(s) 745
AgsI TTSAA 1 cut(s) 361
AjnI CCWGG 4 cut(s) 100, 211, 350, 765
AluBI AGCT 3 cut(s) 801, 811, 816
AluI AGCT 3 cut(s) 801, 811, 816
Alw21I GWGCWC 1 cut(s) 818
Alw26I GTCTC 2 cut(s) 248, 320
AlwI GGATC 3 cut(s) 74, 580, 650
Aor51HI AGCGCT 1 cut(s) 410
AoxI GGCC 4 cut(s) 178, 238, 750, 768
ApeKI GCWGC 1 cut(s) 67
ApoI RAATTY 1 cut(s) 206
AspLEI GCGC 1 cut(s) 411
AsuHPI GGTGA 3 cut(s) 10, 571, 755
BanII GRGCYC 1 cut(s) 818
BbrPI CACGTG 1 cut(s) 746
Bbv12I GWGCWC 1 cut(s) 818
BbvI GCAGC 1 cut(s) 54
BccI CCATC 1 cut(s) 458
BceAI ACGGC 1 cut(s) 225
BciT130I CCWGG 4 cut(s) 102, 213, 352, 767
BcoDI GTCTC 2 cut(s) 248, 320
BfmI CTRYAG 2 cut(s) 6, 195
BfoI RGCGCY 1 cut(s) 412
BfrI CTTAAG 1 cut(s) 425
BglI GCCNNNNNGGC 1 cut(s) 177
BglII AGATCT 1 cut(s) 555
BisI GCNGC 2 cut(s) 68, 181
BlpI GCTNAGC 1 cut(s) 812
BlsI GCNGC 2 cut(s) 69, 182
BmcAI AGTACT 1 cut(s) 63
Bme1390I CCNGG 4 cut(s) 102, 213, 352, 767
BmrFI CCNGG 4 cut(s) 102, 213, 352, 767
BmrI ACTGGG 1 cut(s) 743
BmuI ACTGGG 1 cut(s) 743
BpmI CTGGAG 1 cut(s) 469
Bpu1102I GCTNAGC 1 cut(s) 812
Bsa29I ATCGAT 1 cut(s) 441
BsaAI YACGTR 1 cut(s) 746
BsaI GGTCTC 1 cut(s) 320
BsaJI CCNNGG 3 cut(s) 172, 211, 351
BsaWI WCCGGW 1 cut(s) 115
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 101
Bse118I RCCGGY 3 cut(s) 29, 752, 770
Bse1I ACTGG 3 cut(s) 307, 598, 738
BseBI CCWGG 4 cut(s) 102, 213, 352, 767
BseCI ATCGAT 1 cut(s) 441
BseDI CCNNGG 3 cut(s) 172, 211, 351
BseGI GGATG 1 cut(s) 469
BseLI CCNNNNNNNGG 2 cut(s) 48, 101
BseMII CTCAG 1 cut(s) 803
BseNI ACTGG 3 cut(s) 307, 598, 738
BseRI GAGGAG 1 cut(s) 635
BseXI GCAGC 1 cut(s) 54
Bsh1236I CGCG 1 cut(s) 488
BshFI GGCC 4 cut(s) 180, 240, 752, 770
BshVI ATCGAT 1 cut(s) 441
BsiHKAI GWGCWC 1 cut(s) 818
BsiSI CCGG 4 cut(s) 30, 116, 753, 771
BslI CCNNNNNNNGG 2 cut(s) 48, 101
BsmAI GTCTC 2 cut(s) 248, 320
BsmBI CGTCTC 1 cut(s) 248
BsnI GGCC 4 cut(s) 180, 240, 752, 770
Bso31I GGTCTC 1 cut(s) 320
Bsp1286I GDGCHC 1 cut(s) 818
Bsp1407I TGTACA 1 cut(s) 258
Bsp143I GATC 5 cut(s) 79, 391, 555, 585, 642
Bsp1720I GCTNAGC 1 cut(s) 812
Bsp68I TCGCGA 1 cut(s) 488
BspACI CCGC 3 cut(s) 181, 708, 725
BspANI GGCC 4 cut(s) 180, 240, 752, 770
BspCNI CTCAG 1 cut(s) 804
BspDI ATCGAT 1 cut(s) 441
BspFNI CGCG 1 cut(s) 488
BspMAI CTGCAG 1 cut(s) 10
BspPI GGATC 3 cut(s) 74, 580, 650
BspQI GCTCTTC 1 cut(s) 119
BspTI CTTAAG 1 cut(s) 425
BspTNI GGTCTC 1 cut(s) 320
BsrFI RCCGGY 3 cut(s) 29, 752, 770
BsrGI TGTACA 1 cut(s) 258
BsrI ACTGG 3 cut(s) 307, 598, 738
BssAI RCCGGY 3 cut(s) 29, 752, 770
BssECI CCNNGG 3 cut(s) 172, 211, 351
BssMI GATC 5 cut(s) 79, 391, 555, 585, 642
BssNAI GTATAC 1 cut(s) 470
BssT1I CCWWGG 1 cut(s) 172
Bst1107I GTATAC 1 cut(s) 470
Bst2UI CCWGG 4 cut(s) 102, 213, 352, 767
Bst4CI ACNGT 1 cut(s) 386
Bst6I CTCTTC 2 cut(s) 96, 119
BstAFI CTTAAG 1 cut(s) 425
BstAUI TGTACA 1 cut(s) 258
BstBAI YACGTR 1 cut(s) 746
BstC8I GCNNGC 3 cut(s) 141, 754, 772
BstDEI CTNAG 2 cut(s) 797, 812
BstF5I GGATG 1 cut(s) 469
BstFNI CGCG 1 cut(s) 488
BstH2I RGCGCY 1 cut(s) 412
BstHHI GCGC 1 cut(s) 411
BstKTI GATC 5 cut(s) 82, 394, 558, 588, 645
BstMAI GTCTC 2 cut(s) 248, 320
BstMBI GATC 5 cut(s) 79, 391, 555, 585, 642
BstMWI GCNNNNNNNGC 4 cut(s) 73, 177, 406, 749
BstNI CCWGG 4 cut(s) 102, 213, 352, 767
BstSCI CCNGG 4 cut(s) 100, 211, 350, 765
BstSFI CTRYAG 2 cut(s) 6, 195
BstUI CGCG 1 cut(s) 488
BstV1I GCAGC 1 cut(s) 54
BstX2I RGATCY 3 cut(s) 555, 585, 642
BstYI RGATCY 3 cut(s) 555, 585, 642
BstZ17I GTATAC 1 cut(s) 470
Bsu15I ATCGAT 1 cut(s) 441
BsuRI GGCC 4 cut(s) 180, 240, 752, 770
BsuTUI ATCGAT 1 cut(s) 441
BtsCI GGATG 1 cut(s) 469
BtsIMutI CAGTG 2 cut(s) 591, 698
BtuMI TCGCGA 1 cut(s) 488
Cac8I GCNNGC 3 cut(s) 141, 754, 772
CfoI GCGC 1 cut(s) 411
Cfr10I RCCGGY 3 cut(s) 29, 752, 770
ClaI ATCGAT 1 cut(s) 441
CseI GACGC 1 cut(s) 419
Csp6I GTAC 4 cut(s) 52, 62, 259, 625
CviAII CATG 6 cut(s) 73, 77, 248, 479, 540, 679
CviQI GTAC 4 cut(s) 52, 62, 259, 625
DdeI CTNAG 2 cut(s) 797, 812
DpnI GATC 5 cut(s) 81, 393, 557, 587, 644
DpnII GATC 5 cut(s) 79, 391, 555, 585, 642
EaeI YGGCCR 2 cut(s) 178, 750
Eam1104I CTCTTC 2 cut(s) 96, 119
EarI CTCTTC 2 cut(s) 96, 119
EciI GGCGGA 1 cut(s) 740
Ecl136II GAGCTC 1 cut(s) 816
Eco130I CCWWGG 1 cut(s) 172
Eco24I GRGCYC 1 cut(s) 818
Eco31I GGTCTC 1 cut(s) 320
Eco47III AGCGCT 1 cut(s) 410
Eco53kI GAGCTC 1 cut(s) 816
Eco72I CACGTG 1 cut(s) 746
EcoICRI GAGCTC 1 cut(s) 816
EcoRI GAATTC 1 cut(s) 206
EcoRII CCWGG 4 cut(s) 100, 211, 350, 765
EcoT14I CCWWGG 1 cut(s) 172
EcoT22I ATGCAT 1 cut(s) 78
EcoT38I GRGCYC 1 cut(s) 818
ErhI CCWWGG 1 cut(s) 172
Esp3I CGTCTC 1 cut(s) 248
FaeI CATG 6 cut(s) 76, 80, 251, 482, 543, 682
FatI CATG 6 cut(s) 72, 76, 247, 478, 539, 678
FblI GTMKAC 1 cut(s) 469
Fnu4HI GCNGC 2 cut(s) 68, 181
FokI GGATG 1 cut(s) 476
FriOI GRGCYC 1 cut(s) 818
Fsp4HI GCNGC 2 cut(s) 68, 181
GlaI GCGC 1 cut(s) 410
GluI GCNGC 2 cut(s) 68, 181
GsuI CTGGAG 1 cut(s) 469
HaeII RGCGCY 1 cut(s) 412
HaeIII GGCC 4 cut(s) 180, 240, 752, 770
HapII CCGG 4 cut(s) 30, 116, 753, 771
HgaI GACGC 1 cut(s) 419
HhaI GCGC 1 cut(s) 411
Hin1II CATG 6 cut(s) 76, 80, 251, 482, 543, 682
Hin6I GCGC 1 cut(s) 409
HinP1I GCGC 1 cut(s) 409
HincII GTYRAC 2 cut(s) 537, 789
HindII GTYRAC 2 cut(s) 537, 789
HindIII AAGCTT 1 cut(s) 799
HinfI GANTC 2 cut(s) 438, 760
HpaII CCGG 4 cut(s) 30, 116, 753, 771
HphI GGTGA 3 cut(s) 10, 571, 755
Hpy166II GTNNAC 4 cut(s) 289, 470, 537, 789
Hpy188I TCNGA 3 cut(s) 565, 696, 778
Hpy188III TCNNGA 5 cut(s) 83, 448, 487, 500, 553
Hpy8I GTNNAC 4 cut(s) 289, 470, 537, 789
HpyAV CCTTC 2 cut(s) 483, 625
HpyCH4III ACNGT 1 cut(s) 386
HpyCH4IV ACGT 2 cut(s) 285, 745
HpyCH4V TGCA 3 cut(s) 8, 76, 743
HpyF10VI GCNNNNNNNGC 4 cut(s) 73, 177, 406, 749
HpyF3I CTNAG 2 cut(s) 797, 812
HpySE526I ACGT 2 cut(s) 285, 745
Hsp92II CATG 6 cut(s) 76, 80, 251, 482, 543, 682
HspAI GCGC 1 cut(s) 409
KroI GCCGGC 2 cut(s) 752, 770
KroNI GCCGGC 2 cut(s) 754, 772
Kzo9I GATC 5 cut(s) 79, 391, 555, 585, 642
LguI GCTCTTC 1 cut(s) 119
Lsp1109I GCAGC 1 cut(s) 54
MaeII ACGT 2 cut(s) 285, 745
MaeIII GTNAC 4 cut(s) 230, 420, 431, 761
MalI GATC 5 cut(s) 81, 393, 557, 587, 644
MboI GATC 5 cut(s) 79, 391, 555, 585, 642
MboII GAAGA 5 cut(s) 83, 136, 289, 292, 401
MfeI CAATTG 1 cut(s) 414
MflI RGATCY 3 cut(s) 555, 585, 642
MhlI GDGCHC 1 cut(s) 818
MluCI AATT 6 cut(s) 130, 165, 206, 219, 361, 414
MlyI GAGTC 1 cut(s) 769
MmeI TCCRAC 1 cut(s) 719
MnlI CCTC 7 cut(s) 26, 31, 99, 264, 454, 599, 613
Mph1103I ATGCAT 1 cut(s) 78
MroNI GCCGGC 2 cut(s) 752, 770
MseI TTAA 2 cut(s) 333, 426
MspA1I CMGCKG 1 cut(s) 183
MspCI CTTAAG 1 cut(s) 425
MspI CCGG 4 cut(s) 30, 116, 753, 771
MspR9I CCNGG 4 cut(s) 102, 213, 352, 767
MunI CAATTG 1 cut(s) 414
MvaI CCWGG 4 cut(s) 102, 213, 352, 767
MvnI CGCG 1 cut(s) 488
MwoI GCNNNNNNNGC 4 cut(s) 73, 177, 406, 749
NaeI GCCGGC 2 cut(s) 754, 772
NdeII GATC 5 cut(s) 79, 391, 555, 585, 642
NgoMIV GCCGGC 2 cut(s) 752, 770
NlaIII CATG 6 cut(s) 76, 80, 251, 482, 543, 682
NmuCI GTSAC 3 cut(s) 230, 431, 761
NruI TCGCGA 1 cut(s) 488
NsiI ATGCAT 1 cut(s) 78
PciSI GCTCTTC 1 cut(s) 119
PdiI GCCGGC 2 cut(s) 754, 772
PfeI GAWTC 1 cut(s) 438
PkrI GCNGC 2 cut(s) 69, 182
PleI GAGTC 1 cut(s) 768
PmaCI CACGTG 1 cut(s) 746
PmlI CACGTG 1 cut(s) 746
PpsI GAGTC 1 cut(s) 768
Ppu21I YACGTR 1 cut(s) 746
Psp124BI GAGCTC 1 cut(s) 818
Psp6I CCWGG 4 cut(s) 100, 211, 350, 765
PspCI CACGTG 1 cut(s) 746
PspGI CCWGG 4 cut(s) 100, 211, 350, 765
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 3 cut(s) 555, 585, 642
RruI TCGCGA 1 cut(s) 488
RsaI GTAC 4 cut(s) 53, 63, 260, 626
RsaNI GTAC 4 cut(s) 52, 62, 259, 625
SacI GAGCTC 1 cut(s) 818
SapI GCTCTTC 1 cut(s) 119
SaqAI TTAA 2 cut(s) 333, 426
SatI GCNGC 2 cut(s) 68, 181
Sau3AI GATC 5 cut(s) 79, 391, 555, 585, 642
ScaI AGTACT 1 cut(s) 63
SchI GAGTC 1 cut(s) 769
ScrFI CCNGG 4 cut(s) 102, 213, 352, 767
SduI GDGCHC 1 cut(s) 818
SfcI CTRYAG 2 cut(s) 6, 195
SmlI CTYRAG 1 cut(s) 425
SmoI CTYRAG 1 cut(s) 425
Sse9I AATT 6 cut(s) 130, 165, 206, 219, 361, 414
SsiI CCGC 3 cut(s) 181, 708, 725
SstI GAGCTC 1 cut(s) 818
StyD4I CCNGG 4 cut(s) 100, 211, 350, 765
StyI CCWWGG 1 cut(s) 172
TaaI ACNGT 1 cut(s) 386
TaiI ACGT 2 cut(s) 288, 748
TaqI TCGA 1 cut(s) 441
TasI AATT 6 cut(s) 130, 165, 206, 219, 361, 414
TatI WGTACW 3 cut(s) 61, 258, 624
TauI GCSGC 1 cut(s) 183
TfiI GAWTC 1 cut(s) 438
Tru1I TTAA 2 cut(s) 333, 426
Tru9I TTAA 2 cut(s) 333, 426
TscAI CASTG 2 cut(s) 598, 705
TseFI GTSAC 3 cut(s) 230, 431, 761
TseI GCWGC 1 cut(s) 67
Tsp45I GTSAC 3 cut(s) 230, 431, 761
TspDTI ATGAA 3 cut(s) 290, 698, 793
TspGWI ACGGA 1 cut(s) 450
TspRI CASTG 2 cut(s) 598, 705
Vha464I CTTAAG 1 cut(s) 425
XapI RAATTY 1 cut(s) 206
XmiI GTMKAC 1 cut(s) 469
ZrmI AGTACT 1 cut(s) 63
Zsp2I ATGCAT 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.