pycom09g14790

CCR4-associated factor 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000757
Physical Location & Seq
Reverse (-)
14558077 .. 14559928
1852 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g14790.1

Sequence Viewer

Length: 1071 bp
ATGAAGAGCGTAGCGATTCGACGGGTTTGGCAGCACAACGCAGCATTCGAGTTTCATCTGATTACGTCCTTGATTCGTCACTACACTTTCGTCTCTCTAGACACCGAATTTCCTGGGACAGTTTTCCAAATTCCTGCACAGACGCCTGCATCTAAATACCACCTCATGAGGGAAAACGTGAACGCCACCAAGATAATCCAATTAGGTCTAACGCTCTCCGACCGCCATGGAAACCTTCCGGACTTGGGAACTGACACCTGCTACATTTGGGAGTTCAACTTCAGGGACTTTGATATCGATCGCGACTGTCAAAACAATGACTCAATCGAGTTACTTAAGAGGCAAGGAATCGATTTCTTGGAGAACAAGCAAAATGGTATTAGCGCTTCCTACTTCTCTTCTCTGTTGAGGAATTCCGGTCTGATCTCCAGGGAATCCAACTTGACATGGGTGACATTCCACAGTGCTTATGATTTCGGGTTCTTGATCAAGATTCTAAACGAGGTGTTGCCGCACGACATTACGAGTTTCATGTGGATGATGGATTTATACTTTGGACAAAGAGTGTATGACATCAAATACATGATTAGGTTTTGCCAAGGGCTTCATGGAGGTCTCGAGAAAGTGGCGAATGCACTCAATGTCGATCGTGTGGCCGGAAAGAGTCACCAGGCTGGCTCCGATAGTCTGCTGACTTTGCAAACGTTTATGAAGCTCAAGGATGTGTATTTCAAAACTAGCTTATTGGAACAAAACTGCAACTCAAACTTTTATTGCTGTCAAGGAGTTTTGTATGGCTTGGAAGTTGAGGATCGGAGAGTAGATTTTTTCTTTCCTCTAGACAGCTTTTTGCTCAATCTCTCATGGAGTAAAATTCTTATCAGTACAAGTCAAGGGAATGGCTCATTGTCCTTTTATTTCCATATAAAGGACACGACGAGCATAAATACCCTCTTTAATTTCTATTCTGATGGACTGAATGTCTTTCATAAGGACTCAAAGGAATATGCAACGATTTTTTCCAGGAAATCCCCAACGAAAAATACGCTCTATGACCTCTTTTATATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1 RVT_3

Protein Analysis

357

Amino Acids

41.21

Weight (kDa)

6.41

Isoelectric Point (pI)

31.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 9 - 128 2.6e-11 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 266
Acc36I ACCTGC 1 cut(s) 266
AccII CGCG 1 cut(s) 303
AccIII TCCGGA 1 cut(s) 238
AciI CCGC 2 cut(s) 223, 512
AclI AACGTT 1 cut(s) 704
AclWI GGATC 1 cut(s) 819
AcoI YGGCCR 1 cut(s) 654
AcsI RAATTY 4 cut(s) 107, 129, 412, 873
AcuI CTGAAG 1 cut(s) 265
AcyI GRCGYC 1 cut(s) 143
AfaI GTAC 1 cut(s) 886
AfeI AGCGCT 1 cut(s) 385
AfiI CCNNNNNNNGG 3 cut(s) 169, 245, 928
AflII CTTAAG 1 cut(s) 335
AgsI TTSAA 2 cut(s) 277, 733
AhdI GACNNNNNGTC 1 cut(s) 980
AjnI CCWGG 4 cut(s) 112, 428, 669, 1022
AluBI AGCT 3 cut(s) 715, 741, 846
AluI AGCT 3 cut(s) 715, 741, 846
Alw26I GTCTC 2 cut(s) 97, 620
AlwI GGATC 1 cut(s) 819
Ama87I CYCGRG 1 cut(s) 617
Aor13HI TCCGGA 1 cut(s) 238
Aor51HI AGCGCT 1 cut(s) 385
AoxI GGCC 1 cut(s) 654
ApeKI GCWGC 2 cut(s) 31, 41
ApoI RAATTY 4 cut(s) 107, 129, 412, 873
AspLEI GCGC 1 cut(s) 386
AsuHPI GGTGA 2 cut(s) 463, 659
AvaI CYCGRG 1 cut(s) 617
BarI GAAGNNNNNNTAC 2 cut(s) 370, 402
BbvI GCAGC 2 cut(s) 43, 53
BccI CCATC 2 cut(s) 535, 965
BciT130I CCWGG 4 cut(s) 114, 430, 671, 1024
BclI TGATCA 1 cut(s) 486
BcoDI GTCTC 2 cut(s) 97, 620
BfaI CTAG 3 cut(s) 98, 738, 839
BfoI RGCGCY 1 cut(s) 387
BfrI CTTAAG 1 cut(s) 335
BfuAI ACCTGC 1 cut(s) 266
BisI GCNGC 3 cut(s) 32, 42, 512
BlsI GCNGC 3 cut(s) 33, 43, 513
Bme1390I CCNGG 4 cut(s) 114, 430, 671, 1024
BmeRI GACNNNNNGTC 1 cut(s) 980
BmeT110I CYCGRG 1 cut(s) 617
BmiI GGNNCC 1 cut(s) 679
BmrFI CCNGG 4 cut(s) 114, 430, 671, 1024
BmsI GCATC 1 cut(s) 158
BpmI CTGGAG 1 cut(s) 412
BpuEI CTTGAG 1 cut(s) 701
Bsa29I ATCGAT 2 cut(s) 297, 351
BsaBI GATNNNNATC 1 cut(s) 297
BsaHI GRCGYC 1 cut(s) 143
BsaI GGTCTC 1 cut(s) 620
BsaJI CCNNGG 4 cut(s) 113, 226, 429, 598
BsaWI WCCGGW 2 cut(s) 238, 416
Bsc4I CCNNNNNNNGG 3 cut(s) 169, 245, 928
Bse8I GATNNNNATC 1 cut(s) 297
BseAI TCCGGA 1 cut(s) 238
BseBI CCWGG 4 cut(s) 114, 430, 671, 1024
BseCI ATCGAT 2 cut(s) 297, 351
BseDI CCNNGG 4 cut(s) 113, 226, 429, 598
BseGI GGATG 2 cut(s) 543, 727
BseJI GATNNNNATC 1 cut(s) 297
BseLI CCNNNNNNNGG 3 cut(s) 169, 245, 928
BseXI GCAGC 2 cut(s) 43, 53
BsgI GTGCAG 1 cut(s) 120
Bsh1236I CGCG 1 cut(s) 303
Bsh1285I CGRYCG 3 cut(s) 223, 301, 649
BshFI GGCC 1 cut(s) 656
BshVI ATCGAT 2 cut(s) 297, 351
BsiEI CGRYCG 3 cut(s) 223, 301, 649
BsiHKCI CYCGRG 1 cut(s) 617
BsiSI CCGG 3 cut(s) 239, 417, 657
BslFI GGGAC 2 cut(s) 130, 299
BslI CCNNNNNNNGG 3 cut(s) 169, 245, 928
BsmAI GTCTC 2 cut(s) 97, 620
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 2 cut(s) 130, 299
BsmI GAATGC 2 cut(s) 44, 637
BsnI GGCC 1 cut(s) 656
Bso31I GGTCTC 1 cut(s) 620
BsoBI CYCGRG 1 cut(s) 617
Bsp13I TCCGGA 1 cut(s) 238
Bsp143I GATC 5 cut(s) 298, 423, 486, 646, 811
Bsp19I CCATGG 1 cut(s) 226
Bsp68I TCGCGA 1 cut(s) 303
BspACI CCGC 2 cut(s) 223, 512
BspANI GGCC 1 cut(s) 656
BspDI ATCGAT 2 cut(s) 297, 351
BspEI TCCGGA 1 cut(s) 238
BspFNI CGCG 1 cut(s) 303
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 1 cut(s) 679
BspMI ACCTGC 1 cut(s) 266
BspPI GGATC 1 cut(s) 819
BspTI CTTAAG 1 cut(s) 335
BspTNI GGTCTC 1 cut(s) 620
BssECI CCNNGG 4 cut(s) 113, 226, 429, 598
BssMI GATC 5 cut(s) 298, 423, 486, 646, 811
BssNI GRCGYC 1 cut(s) 143
BssT1I CCWWGG 2 cut(s) 226, 598
Bst2UI CCWGG 4 cut(s) 114, 430, 671, 1024
Bst4CI ACNGT 3 cut(s) 121, 308, 464
Bst6I CTCTTC 1 cut(s) 403
BstACI GRCGYC 1 cut(s) 143
BstAFI CTTAAG 1 cut(s) 335
BstC8I GCNNGC 2 cut(s) 147, 676
BstDSI CCRYGG 1 cut(s) 226
BstF5I GGATG 2 cut(s) 543, 727
BstFNI CGCG 1 cut(s) 303
BstH2I RGCGCY 1 cut(s) 387
BstHHI GCGC 1 cut(s) 386
BstKTI GATC 5 cut(s) 301, 426, 489, 649, 814
BstMAI GTCTC 2 cut(s) 97, 620
BstMBI GATC 5 cut(s) 298, 423, 486, 646, 811
BstMCI CGRYCG 3 cut(s) 223, 301, 649
BstMWI GCNNNNNNNGC 1 cut(s) 697
BstNI CCWGG 4 cut(s) 114, 430, 671, 1024
BstSCI CCNGG 4 cut(s) 112, 428, 669, 1022
BstUI CGCG 1 cut(s) 303
BstV1I GCAGC 2 cut(s) 43, 53
Bsu15I ATCGAT 2 cut(s) 297, 351
BsuRI GGCC 1 cut(s) 656
BsuTUI ATCGAT 2 cut(s) 297, 351
BtgI CCRYGG 1 cut(s) 226
BtsCI GGATG 2 cut(s) 543, 727
BtsIMutI CAGTG 1 cut(s) 469
BtuMI TCGCGA 1 cut(s) 303
BveI ACCTGC 1 cut(s) 266
Cac8I GCNNGC 2 cut(s) 147, 676
CciI TCATGA 1 cut(s) 165
CfoI GCGC 1 cut(s) 386
ClaI ATCGAT 2 cut(s) 297, 351
CseI GACGC 1 cut(s) 151
Csp6I GTAC 1 cut(s) 885
CviAII CATG 7 cut(s) 166, 227, 447, 532, 583, 608, 864
CviJI RGCY 9 cut(s) 604, 656, 674, 678, 715, 741, 798, 846, 903
CviKI_1 RGCY 9 cut(s) 604, 656, 674, 678, 715, 741, 798, 846, 903
CviQI GTAC 1 cut(s) 885
DpnI GATC 5 cut(s) 300, 425, 488, 648, 813
DpnII GATC 5 cut(s) 298, 423, 486, 646, 811
DriI GACNNNNNGTC 1 cut(s) 980
EaeI YGGCCR 1 cut(s) 654
Eam1104I CTCTTC 1 cut(s) 403
Eam1105I GACNNNNNGTC 1 cut(s) 980
EarI CTCTTC 1 cut(s) 403
Eco130I CCWWGG 2 cut(s) 226, 598
Eco31I GGTCTC 1 cut(s) 620
Eco32I GATATC 1 cut(s) 295
Eco47III AGCGCT 1 cut(s) 385
Eco57I CTGAAG 1 cut(s) 265
Eco88I CYCGRG 1 cut(s) 617
EcoRI GAATTC 1 cut(s) 412
EcoRII CCWGG 4 cut(s) 112, 428, 669, 1022
EcoRV GATATC 1 cut(s) 295
EcoT14I CCWWGG 2 cut(s) 226, 598
ErhI CCWWGG 2 cut(s) 226, 598
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 7 cut(s) 169, 230, 450, 535, 586, 611, 867
FaqI GGGAC 2 cut(s) 130, 299
FatI CATG 7 cut(s) 165, 226, 446, 531, 582, 607, 863
FbaI TGATCA 1 cut(s) 486
Fnu4HI GCNGC 3 cut(s) 32, 42, 512
FokI GGATG 2 cut(s) 550, 734
Fsp4HI GCNGC 3 cut(s) 32, 42, 512
FspBI CTAG 3 cut(s) 98, 738, 839
GlaI GCGC 1 cut(s) 385
GluI GCNGC 3 cut(s) 32, 42, 512
GsuI CTGGAG 1 cut(s) 412
HaeII RGCGCY 1 cut(s) 387
HaeIII GGCC 1 cut(s) 656
HapII CCGG 3 cut(s) 239, 417, 657
HgaI GACGC 1 cut(s) 151
HhaI GCGC 1 cut(s) 386
Hin1I GRCGYC 1 cut(s) 143
Hin1II CATG 7 cut(s) 169, 230, 450, 535, 586, 611, 867
Hin6I GCGC 1 cut(s) 384
HinP1I GCGC 1 cut(s) 384
HinfI GANTC 8 cut(s) 16, 73, 320, 348, 434, 493, 664, 995
HpaII CCGG 3 cut(s) 239, 417, 657
HphI GGTGA 2 cut(s) 463, 659
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 6 cut(s) 60, 220, 423, 682, 816, 970
Hpy188III TCNNGA 9 cut(s) 98, 166, 239, 302, 484, 490, 617, 619, 839
Hpy8I GTNNAC 1 cut(s) 181
Hpy99I CGWCG 2 cut(s) 24, 940
HpyAV CCTTC 1 cut(s) 245
HpyCH4III ACNGT 3 cut(s) 121, 308, 464
HpyCH4IV ACGT 3 cut(s) 65, 177, 704
HpyCH4V TGCA 6 cut(s) 137, 149, 635, 700, 759, 1010
HpyF10VI GCNNNNNNNGC 1 cut(s) 697
HpySE526I ACGT 3 cut(s) 65, 177, 704
Hsp92I GRCGYC 1 cut(s) 143
Hsp92II CATG 7 cut(s) 169, 230, 450, 535, 586, 611, 867
HspAI GCGC 1 cut(s) 384
Kpn2I TCCGGA 1 cut(s) 238
Ksp22I TGATCA 1 cut(s) 486
Kzo9I GATC 5 cut(s) 298, 423, 486, 646, 811
LmnI GCTCC 1 cut(s) 683
Lsp1109I GCAGC 2 cut(s) 43, 53
LweI GCATC 1 cut(s) 158
MaeI CTAG 3 cut(s) 98, 738, 839
MaeII ACGT 3 cut(s) 65, 177, 704
MaeIII GTNAC 4 cut(s) 77, 330, 451, 665
MalI GATC 5 cut(s) 300, 425, 488, 648, 813
MboI GATC 5 cut(s) 298, 423, 486, 646, 811
MboII GAAGA 2 cut(s) 16, 390
MluCI AATT 6 cut(s) 107, 129, 200, 412, 873, 958
MlyI GAGTC 3 cut(s) 314, 673, 989
MmeI TCCRAC 2 cut(s) 243, 462
MroI TCCGGA 1 cut(s) 238
MseI TTAA 3 cut(s) 336, 957, 1069
MslI CAYNNNNRTG 1 cut(s) 536
MspCI CTTAAG 1 cut(s) 335
MspI CCGG 3 cut(s) 239, 417, 657
MspR9I CCNGG 4 cut(s) 114, 430, 671, 1024
Mva1269I GAATGC 2 cut(s) 44, 637
MvaI CCWGG 4 cut(s) 114, 430, 671, 1024
MvnI CGCG 1 cut(s) 303
MwoI GCNNNNNNNGC 1 cut(s) 697
NcoI CCATGG 1 cut(s) 226
NdeII GATC 5 cut(s) 298, 423, 486, 646, 811
NlaIII CATG 7 cut(s) 169, 230, 450, 535, 586, 611, 867
NlaIV GGNNCC 1 cut(s) 679
NmuCI GTSAC 3 cut(s) 77, 451, 665
NruI TCGCGA 1 cut(s) 303
PaeR7I CTCGAG 1 cut(s) 617
PagI TCATGA 1 cut(s) 165
PaqCI CACCTGC 1 cut(s) 266
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PctI GAATGC 2 cut(s) 44, 637
PfeI GAWTC 5 cut(s) 16, 73, 348, 434, 493
PfoI TCCNGGA 1 cut(s) 1022
PkrI GCNGC 3 cut(s) 33, 43, 513
Ple19I CGATCG 2 cut(s) 301, 649
PleI GAGTC 3 cut(s) 314, 672, 989
PpsI GAGTC 3 cut(s) 314, 672, 989
Psp1406I AACGTT 1 cut(s) 704
Psp6I CCWGG 4 cut(s) 112, 428, 669, 1022
PspGI CCWGG 4 cut(s) 112, 428, 669, 1022
PspN4I GGNNCC 1 cut(s) 679
PvuI CGATCG 2 cut(s) 301, 649
RruI TCGCGA 1 cut(s) 303
RsaI GTAC 1 cut(s) 886
RsaNI GTAC 1 cut(s) 885
RseI CAYNNNNRTG 1 cut(s) 536
SaqAI TTAA 3 cut(s) 336, 957, 1069
SatI GCNGC 3 cut(s) 32, 42, 512
Sau3AI GATC 5 cut(s) 298, 423, 486, 646, 811
SchI GAGTC 3 cut(s) 314, 673, 989
ScrFI CCNGG 4 cut(s) 114, 430, 671, 1024
SfaNI GCATC 1 cut(s) 158
Sfr274I CTCGAG 1 cut(s) 617
SlaI CTCGAG 1 cut(s) 617
SmiMI CAYNNNNRTG 1 cut(s) 536
SmlI CTYRAG 3 cut(s) 335, 617, 716
SmoI CTYRAG 3 cut(s) 335, 617, 716
Sse9I AATT 6 cut(s) 107, 129, 200, 412, 873, 958
SsiI CCGC 2 cut(s) 223, 512
SspMI CTAG 3 cut(s) 98, 738, 839
StyD4I CCNGG 4 cut(s) 112, 428, 669, 1022
StyI CCWWGG 2 cut(s) 226, 598
TaaI ACNGT 3 cut(s) 121, 308, 464
TaiI ACGT 3 cut(s) 68, 180, 707
TaqI TCGA 7 cut(s) 19, 48, 297, 327, 351, 618, 645
TasI AATT 6 cut(s) 107, 129, 200, 412, 873, 958
TatI WGTACW 1 cut(s) 884
TauI GCSGC 1 cut(s) 514
TfiI GAWTC 5 cut(s) 16, 73, 348, 434, 493
Tru1I TTAA 3 cut(s) 336, 957, 1069
Tru9I TTAA 3 cut(s) 336, 957, 1069
TscAI CASTG 1 cut(s) 469
TseFI GTSAC 3 cut(s) 77, 451, 665
TseI GCWGC 2 cut(s) 31, 41
Tsp45I GTSAC 3 cut(s) 77, 451, 665
TspDTI ATGAA 6 cut(s) 17, 44, 520, 596, 725, 977
TspRI CASTG 1 cut(s) 469
Vha464I CTTAAG 1 cut(s) 335
XapI RAATTY 4 cut(s) 107, 129, 412, 873
XbaI TCTAGA 2 cut(s) 97, 838
XcmI CCANNNNNNNNNTGG 1 cut(s) 605
XhoI CTCGAG 1 cut(s) 617
XspI CTAG 3 cut(s) 98, 738, 839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.