RchiOBHm_Chr2g0134461

CCR4-associated factor 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000757
Physical Location & Seq
Reverse (-)
51593421 .. 51594079
659 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50549

Sequence Viewer

Length: 618 bp
ATGATGAAGACCAATGTGGACCAAACCCATATTATCCAATTGGGTCTTTCACTCTCTGCCCATTTGGGAATTTGCCGTGATTTTGATGTGGACTGTGCTCAACATTTACGGAATAGTGTCTCGATTGATGTCCTGAAGCAGCAGGGAATAGACTTTTCCGACAACAAATGCACTGGTATTTGTTCTGCAGACTTTGCTGAGGAGATTGAGAGGTCTGGGCTAGTGGAGTTACTTCCTTGCGTGACTTGGGCTACCTTCCAGAGTGCATATGATTTTGGGTACTTGATAAAGATGTTCACTGGGAACAAGGAATTGCCAGAGGATATCAAGGAGTTCATGGGCAATGTGAAGACATATTTTGGACCGAATGTGTACGACATCAAGTACATGATCAAGTTCTGTGATGGACTGTTTGGCGGTTTGAATAGCGTGGCAGCAACCCTTGGCGTTGACCGTGTGGCTGGGAGTAGCCACCAGGCTGGTTCTAATAGTCTTTTGACACTGCAAACGTTTGTCAAGATGCTCAAGATGGAGAAGAACAAAGGGAAAGAGCTCTGGGAAGAATGTGGAGGAGTTTTGTTTGGTTTAGGAAGCTTGAATTTCTGGCATCATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1 RVT_3

Protein Analysis

205

Amino Acids

22.77

Weight (kDa)

5.47

Isoelectric Point (pI)

38.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 89 - 133 1.7e-06 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 417
AclI AACGTT 1 cut(s) 509
AcsI RAATTY 2 cut(s) 69, 598
AcuI CTGAAG 1 cut(s) 155
AfaI GTAC 3 cut(s) 281, 374, 386
AgsI TTSAA 2 cut(s) 424, 598
AjnI CCWGG 1 cut(s) 474
AluBI AGCT 2 cut(s) 553, 594
AluI AGCT 2 cut(s) 553, 594
Alw21I GWGCWC 2 cut(s) 100, 555
Alw26I GTCTC 1 cut(s) 124
ApeKI GCWGC 2 cut(s) 139, 434
ApoI RAATTY 2 cut(s) 69, 598
AspS9I GGNCC 2 cut(s) 19, 362
AvaII GGWCC 2 cut(s) 19, 362
BanII GRGCYC 1 cut(s) 555
BbsI GAAGAC 2 cut(s) 14, 356
Bbv12I GWGCWC 2 cut(s) 100, 555
BbvCI CCTCAGC 1 cut(s) 198
BbvI GCAGC 2 cut(s) 151, 446
BccI CCATC 2 cut(s) 398, 523
BceAI ACGGC 1 cut(s) 60
BciT130I CCWGG 1 cut(s) 476
BclI TGATCA 1 cut(s) 390
BcoDI GTCTC 1 cut(s) 124
BfaI CTAG 1 cut(s) 221
BfmI CTRYAG 1 cut(s) 186
BisI GCNGC 2 cut(s) 140, 435
BlsI GCNGC 2 cut(s) 141, 436
Bme1390I CCNGG 1 cut(s) 476
Bme18I GGWCC 2 cut(s) 19, 362
BmgT120I GGNCC 2 cut(s) 19, 362
BmrFI CCNGG 1 cut(s) 476
BmrI ACTGGG 1 cut(s) 309
BmsI GCATC 1 cut(s) 510
BmuI ACTGGG 1 cut(s) 309
BpiI GAAGAC 2 cut(s) 14, 356
Bpu10I CCTNAGC 1 cut(s) 198
BpuEI CTTGAG 1 cut(s) 509
BsaJI CCNNGG 1 cut(s) 442
Bse1I ACTGG 2 cut(s) 178, 304
Bse3DI GCAATG 1 cut(s) 349
BseBI CCWGG 1 cut(s) 476
BseDI CCNNGG 1 cut(s) 442
BseMI GCAATG 1 cut(s) 349
BseMII CTCAG 1 cut(s) 189
BseNI ACTGG 2 cut(s) 178, 304
BseRI GAGGAG 2 cut(s) 215, 585
BseXI GCAGC 2 cut(s) 151, 446
BseYI CCCAGC 1 cut(s) 461
BsiHKAI GWGCWC 2 cut(s) 100, 555
BsmAI GTCTC 1 cut(s) 124
Bsp1286I GDGCHC 2 cut(s) 100, 555
Bsp143I GATC 1 cut(s) 390
BspACI CCGC 1 cut(s) 417
BspCNI CTCAG 1 cut(s) 190
BspMAI CTGCAG 1 cut(s) 190
BsrDI GCAATG 1 cut(s) 349
BsrI ACTGG 2 cut(s) 178, 304
BssECI CCNNGG 1 cut(s) 442
BssMI GATC 1 cut(s) 390
BssT1I CCWWGG 1 cut(s) 442
Bst2UI CCWGG 1 cut(s) 476
Bst4CI ACNGT 3 cut(s) 95, 411, 455
BstAPI GCANNNNNTGC 1 cut(s) 194
BstDEI CTNAG 1 cut(s) 198
BstKTI GATC 1 cut(s) 393
BstMAI GTCTC 1 cut(s) 124
BstMBI GATC 1 cut(s) 390
BstMWI GCNNNNNNNGC 1 cut(s) 194
BstNI CCWGG 1 cut(s) 476
BstSCI CCNGG 1 cut(s) 474
BstSFI CTRYAG 1 cut(s) 186
BstV1I GCAGC 2 cut(s) 151, 446
BstV2I GAAGAC 2 cut(s) 14, 356
BstXI CCANNNNNNTGG 1 cut(s) 479
BtsI GCAGTG 1 cut(s) 500
BtsIMutI CAGTG 3 cut(s) 171, 297, 500
Cfr13I GGNCC 2 cut(s) 19, 362
Csp6I GTAC 3 cut(s) 280, 373, 385
CviAII CATG 2 cut(s) 337, 388
CviJI RGCY 7 cut(s) 220, 251, 461, 471, 479, 553, 594
CviKI_1 RGCY 7 cut(s) 220, 251, 461, 471, 479, 553, 594
CviQI GTAC 3 cut(s) 280, 373, 385
DdeI CTNAG 1 cut(s) 198
DpnI GATC 1 cut(s) 392
DpnII GATC 1 cut(s) 390
Ecl136II GAGCTC 1 cut(s) 553
Eco130I CCWWGG 1 cut(s) 442
Eco24I GRGCYC 1 cut(s) 555
Eco32I GATATC 1 cut(s) 325
Eco47I GGWCC 2 cut(s) 19, 362
Eco53kI GAGCTC 1 cut(s) 553
Eco57I CTGAAG 1 cut(s) 155
EcoICRI GAGCTC 1 cut(s) 553
EcoRII CCWGG 1 cut(s) 474
EcoRV GATATC 1 cut(s) 325
EcoT14I CCWWGG 1 cut(s) 442
EcoT38I GRGCYC 1 cut(s) 555
ErhI CCWWGG 1 cut(s) 442
FaeI CATG 2 cut(s) 340, 391
FaiI YATR 6 cut(s) 30, 268, 270, 338, 355, 389
FatI CATG 2 cut(s) 336, 387
FauNDI CATATG 1 cut(s) 268
FbaI TGATCA 1 cut(s) 390
Fnu4HI GCNGC 2 cut(s) 140, 435
FriOI GRGCYC 1 cut(s) 555
Fsp4HI GCNGC 2 cut(s) 140, 435
FspBI CTAG 1 cut(s) 221
GluI GCNGC 2 cut(s) 140, 435
GsaI CCCAGC 1 cut(s) 465
Hin1II CATG 2 cut(s) 340, 391
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HindIII AAGCTT 1 cut(s) 592
Hpy166II GTNNAC 5 cut(s) 19, 91, 297, 373, 451
Hpy188I TCNGA 1 cut(s) 160
Hpy188III TCNNGA 5 cut(s) 121, 133, 259, 517, 526
Hpy8I GTNNAC 5 cut(s) 19, 91, 297, 373, 451
HpyAV CCTTC 1 cut(s) 265
HpyCH4III ACNGT 3 cut(s) 95, 411, 455
HpyCH4IV ACGT 1 cut(s) 509
HpyCH4V TGCA 4 cut(s) 171, 188, 266, 505
HpyF10VI GCNNNNNNNGC 1 cut(s) 194
HpyF3I CTNAG 1 cut(s) 198
HpySE526I ACGT 1 cut(s) 509
Hsp92II CATG 2 cut(s) 340, 391
Ksp22I TGATCA 1 cut(s) 390
Kzo9I GATC 1 cut(s) 390
Lsp1109I GCAGC 2 cut(s) 151, 446
LweI GCATC 1 cut(s) 510
MaeI CTAG 1 cut(s) 221
MaeII ACGT 1 cut(s) 509
MaeIII GTNAC 2 cut(s) 228, 241
MalI GATC 1 cut(s) 392
MboI GATC 1 cut(s) 390
MboII GAAGA 4 cut(s) 19, 361, 547, 572
MfeI CAATTG 1 cut(s) 38
MhlI GDGCHC 2 cut(s) 100, 555
MluCI AATT 4 cut(s) 38, 69, 311, 598
MmeI TCCRAC 1 cut(s) 183
MnlI CCTC 4 cut(s) 193, 204, 313, 563
MspR9I CCNGG 1 cut(s) 476
MunI CAATTG 1 cut(s) 38
MvaI CCWGG 1 cut(s) 476
MwoI GCNNNNNNNGC 1 cut(s) 194
NdeI CATATG 1 cut(s) 268
NdeII GATC 1 cut(s) 390
NlaIII CATG 2 cut(s) 340, 391
NmuCI GTSAC 1 cut(s) 241
PkrI GCNGC 2 cut(s) 141, 436
Psp124BI GAGCTC 1 cut(s) 555
Psp1406I AACGTT 1 cut(s) 509
Psp6I CCWGG 1 cut(s) 474
PspFI CCCAGC 1 cut(s) 461
PspGI CCWGG 1 cut(s) 474
PspPI GGNCC 2 cut(s) 19, 362
PstI CTGCAG 1 cut(s) 190
RsaI GTAC 3 cut(s) 281, 374, 386
RsaNI GTAC 3 cut(s) 280, 373, 385
SacI GAGCTC 1 cut(s) 555
SatI GCNGC 2 cut(s) 140, 435
Sau3AI GATC 1 cut(s) 390
Sau96I GGNCC 2 cut(s) 19, 362
ScrFI CCNGG 1 cut(s) 476
SduI GDGCHC 2 cut(s) 100, 555
SetI ASST 5 cut(s) 215, 257, 512, 555, 596
SfaNI GCATC 1 cut(s) 510
SfcI CTRYAG 1 cut(s) 186
SinI GGWCC 2 cut(s) 19, 362
SmlI CTYRAG 1 cut(s) 524
SmoI CTYRAG 1 cut(s) 524
Sse9I AATT 4 cut(s) 38, 69, 311, 598
SsiI CCGC 1 cut(s) 417
SspMI CTAG 1 cut(s) 221
SstI GAGCTC 1 cut(s) 555
StyD4I CCNGG 1 cut(s) 474
StyI CCWWGG 1 cut(s) 442
TaaI ACNGT 3 cut(s) 95, 411, 455
TaiI ACGT 1 cut(s) 512
TaqI TCGA 1 cut(s) 122
TaqII GACCGA 1 cut(s) 379
TasI AATT 4 cut(s) 38, 69, 311, 598
TatI WGTACW 1 cut(s) 384
TscAI CASTG 3 cut(s) 178, 304, 507
TseFI GTSAC 1 cut(s) 241
TseI GCWGC 2 cut(s) 139, 434
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 2 cut(s) 20, 325
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 3 cut(s) 178, 304, 507
VpaK11BI GGWCC 2 cut(s) 19, 362
XapI RAATTY 2 cut(s) 69, 598
XspI CTAG 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.