Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0000757

Physical Location & Seq

Genomic Coordinates Reverse (-)

9237718 .. 9238734

Gene Structure 1017 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1080800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1017 bp

ATGGCCAACAGATTCACGGCCGTTTGGGCCGACAATTTTGCACAGGAAATCGCCGCCGTCGATCGCTTTCTCACCCACTTCCCCATCGTCTCCTTCGACGCGGAGTTTCCCGGATTTCTCCGCAACACGCCCAGAAGTGCTTCCGACGCTCTCCGCTACCAAGATCTCCGCTTTAACGTCGATTCCCTCAAACTCATCCAATTGGGTATCACCCTCTTCGACGATCTCGGCAACATCGGGGGCACCTGGGAGTTCAATTTCCGGGGTTTTGACGAGGAAACCGACCCGCACGTCGCCGATTCCATCACCCTTCTCAAAGCCAACGGCTTGGATCTCGAGAAATTCCGGAAATTCGGAATCGACCCGGAGGTTTTCGAGGAGGGTTTTGCCGAGGTTTTGCGGATGCACAGAGGGCGCCTCTTATGGGTGAGCTTCCACGGCCTCTATGACTCGGCCTATGTGATGAAGTTCATGACCCGGAAGGCCATGCCGAGCTCACCTACCGAGTTCGCCGCGATGGCGGGAAATCTATTTGAGAGGGTGTGTGACCTGAAATTTATGGCCAAATTTTACCAGGGCCTGCTTGGGGGCGAAATTGGGCTCGAGAGGATGGCGAAAATCCTCCGAGTGCAGCGTTACGGCGATGCCCACCAAGCGGGTTCCGATAGTTTGTTAACGGCTGATGCATTTGTGAGGATGAGGGCTCAATTTGTGTTGAAGCCAGGGATGTATGAGGGGTTTCTATATGGGTTAAGTCCAAAGATTTGTGCTTCTTCTGTACACAGGGTTAGAGCTCCTCCTGGTCAGGTTATCCTTGTCCCTGTTGTACCCACTAGACCTGTCATTCTCGTCCGCCACTCCTCCTCCTCCCGTGATCAGTCCTTCCAGAGCCATCACCAGGCTTCGCCTATGCTCTATCCTTATCCTTCTCCAGCTCCGATGCTTATGCATAGGCCGATGTGCATTCCTTTTCATGCCTTGCCTCAAGCCCCTCCCCACTATCAGATTCAAATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000175 GO:0000288 GO:0000291 GO:0000932 GO:0000956 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004535 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008408 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0017148 GO:0019222 GO:0019439 GO:0030014 GO:0030015 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032268 GO:0032269 GO:0032991 GO:0034248 GO:0034249 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1990904 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

CAF1

Protein Analysis

339

Amino Acids

38.24

Weight (kDa)

7.81

Isoelectric Point (pI)

48.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CAF1	PF04857	8 - 120	4.1e-08	CAF1 family ribonuclease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000757)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06450 AT1G27820 AT1G27890 AT1G61470 AT3G44240
fragaria_vesca	FvH4_6g28110 FvH4_6g28140 FvH4_6g29280 FvH4_6g29280
malus_domestica	MD03G1041900.v1.1 MD09G1229300.v1.1 MD11G1042700.v1.1 MD12G1082100.v1.1 MD14G1080800.v1.1
prunus_persica	Prupe.1G188200_v2.0.a1 Prupe.1G190800_v2.0.a1 Prupe.1G190900_v2.0.a1 Prupe.3G105100_v2.0.a1 Prupe.7G010200_v2.0.a1
pyrus_communis	pycom09g14790 pycom14g07010
rosa_chinensis	RchiOBHm_Chr2g0132091 RchiOBHm_Chr2g0132101 RchiOBHm_Chr2g0134441 RchiOBHm_Chr2g0134461 RchiOBHm_Chr2g0136851 RchiOBHm_Chr2g0167821 RchiOBHm_Chr2g0167861 RchiOBHm_Chr2g0167901 RchiOBHm_Chr2g0167921 RchiOBHm_Chr6g0266841
rosa_laevigata	RLG00000019225 RLG00000019226 RLG00000019393
rosa_multiflora	Rmu_sc0001299.1_g000004 Rmu_sc0005571.1_g000018
rosa_roxburghii	Rroxscaffold_2G00110210 Rroxscaffold_2G00110240 Rroxscaffold_2G00112400 Rroxscaffold_3G00239330 Rroxscaffold_4G00313080
rosa_rugosa	Rorug02G0302000 Rorug02G0302200 Rorug02G0317200 Rorug02G0317200 Rorug03G0220600
rosa_samantha	Rh2AG355100 Rh2AG355200 Rh2AG369800 Rh2AG369900 Rh2AG384800 Rh2CG338700 Rh2CG338800 Rh2CG353700 Rh2CG371400 Rh2DG370100 Rh2DG377900 Rh2DG378000 Rh2DG392800 Rh2DG392900 Rh2DG408200 Rh6BG148800 Rh6BG149300 Rh6CG159200 Rh6DG133000 Rh6DG133400
rosa_wichuraiana	Rw1G013790 Rw2G028830 Rw2G028840 Rw2G031470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	290
AccB1I	GGYRCC	2 cut(s)	242, 414
AccII	CGCG	2 cut(s)	101, 515
AccIII	TCCGGA	1 cut(s)	345
AclWI	GGATC	1 cut(s)	339
AcoI	YGGCCR	3 cut(s)	3, 18, 561
AcsI	RAATTY	4 cut(s)	341, 350, 554, 566
AcyI	GRCGYC	1 cut(s)	415
AfaI	GTAC	2 cut(s)	780, 828
AfiI	CCNNNNNNNGG	4 cut(s)	424, 586, 655, 898
AgsI	TTSAA	3 cut(s)	256, 718, 1010
AjiI	CACGTC	1 cut(s)	292
AjnI	CCWGG	5 cut(s)	245, 573, 721, 799, 897
AluBI	AGCT	4 cut(s)	432, 495, 794, 935
AluI	AGCT	4 cut(s)	432, 495, 794, 935
Alw21I	GWGCWC	2 cut(s)	497, 796
Alw26I	GTCTC	1 cut(s)	94
AlwI	GGATC	1 cut(s)	339
AlwNI	CAGNNNCTG	1 cut(s)	580
Ama87I	CYCGRG	2 cut(s)	335, 602
Aor13HI	TCCGGA	1 cut(s)	345
AoxI	GGCC	9 cut(s)	3, 18, 27, 439, 453, 483, 561, 577, 953
ApeKI	GCWGC	1 cut(s)	631
ApoI	RAATTY	4 cut(s)	341, 350, 554, 566
Asp700I	GAANNNNTTC	1 cut(s)	139
AspLEI	GCGC	1 cut(s)	417
AspS9I	GGNCC	2 cut(s)	27, 577
AsuC2I	CCSGG	4 cut(s)	111, 263, 365, 478
AsuHPI	GGTGA	6 cut(s)	64, 202, 298, 439, 489, 887
AvaI	CYCGRG	2 cut(s)	335, 602
BaeGI	GKGCMC	1 cut(s)	245
BalI	TGGCCA	2 cut(s)	5, 563
BanI	GGYRCC	2 cut(s)	242, 414
BanII	GRGCYC	4 cut(s)	497, 603, 706, 796
Bbv12I	GWGCWC	2 cut(s)	497, 796
BbvI	GCAGC	1 cut(s)	643
BccI	CCATC	5 cut(s)	92, 311, 511, 604, 900
BceAI	ACGGC	7 cut(s)	5, 33, 41, 340, 454, 655, 693
BcgI	CGANNNNNNTGC	4 cut(s)	20, 54, 928, 962
BciT130I	CCWGG	5 cut(s)	247, 575, 723, 801, 899
BclI	TGATCA	1 cut(s)	874
BcnI	CCSGG	4 cut(s)	111, 263, 365, 478
BcoDI	GTCTC	1 cut(s)	94
BfaI	CTAG	1 cut(s)	834
BfoI	RGCGCY	1 cut(s)	418
BglI	GCCNNNNNGGC	2 cut(s)	26, 518
BglII	AGATCT	1 cut(s)	163
BisI	GCNGC	3 cut(s)	54, 513, 632
BlsI	GCNGC	3 cut(s)	55, 514, 633
Bme1390I	CCNGG	9 cut(s)	111, 247, 263, 365, 478, 575, 723, 801, 899
BmeT110I	CYCGRG	2 cut(s)	335, 602
BmgBI	CACGTC	1 cut(s)	292
BmgT120I	GGNCC	2 cut(s)	27, 577
BmiI	GGNNCC	3 cut(s)	244, 416, 661
BmrFI	CCNGG	9 cut(s)	111, 247, 263, 365, 478, 575, 723, 801, 899
BmsI	GCATC	4 cut(s)	393, 634, 673, 930
BplI	GAGNNNNNCTC	2 cut(s)	402, 434
BpmI	CTGGAG	1 cut(s)	915
BpuEI	CTTGAG	1 cut(s)	969
BpuMI	CCSGG	4 cut(s)	111, 263, 365, 478
BsaHI	GRCGYC	1 cut(s)	415
BsaJI	CCNNGG	6 cut(s)	246, 262, 390, 436, 574, 722
BsaWI	WCCGGW	1 cut(s)	345
BsaXI	ACNNNNNCTCC	2 cut(s)	848, 878
Bsc4I	CCNNNNNNNGG	4 cut(s)	424, 586, 655, 898
BseAI	TCCGGA	1 cut(s)	345
BseBI	CCWGG	5 cut(s)	247, 575, 723, 801, 899
BseDI	CCNNGG	6 cut(s)	246, 262, 390, 436, 574, 722
BseGI	GGATG	5 cut(s)	195, 408, 615, 702, 732
BseLI	CCNNNNNNNGG	4 cut(s)	424, 586, 655, 898
BseRI	GAGGAG	5 cut(s)	392, 786, 850, 853, 856
BseSI	GKGCMC	1 cut(s)	245
BseX3I	CGGCCG	1 cut(s)	18
BseXI	GCAGC	1 cut(s)	643
BsgI	GTGCAG	1 cut(s)	650
Bsh1236I	CGCG	2 cut(s)	101, 515
Bsh1285I	CGRYCG	2 cut(s)	21, 64
BshFI	GGCC	9 cut(s)	5, 20, 29, 441, 455, 485, 563, 579, 955
BshNI	GGYRCC	2 cut(s)	242, 414
BsiEI	CGRYCG	2 cut(s)	21, 64
BsiHKAI	GWGCWC	2 cut(s)	497, 796
BsiHKCI	CYCGRG	2 cut(s)	335, 602
BsiSI	CCGG	5 cut(s)	111, 262, 346, 365, 478
BslFI	GGGAC	1 cut(s)	803
BslI	CCNNNNNNNGG	4 cut(s)	424, 586, 655, 898
BsmAI	GTCTC	1 cut(s)	94
BsmBI	CGTCTC	1 cut(s)	94
BsmFI	GGGAC	1 cut(s)	803
BsmI	GAATGC	1 cut(s)	963
BsnI	GGCC	9 cut(s)	5, 20, 29, 441, 455, 485, 563, 579, 955
BsoBI	CYCGRG	2 cut(s)	335, 602
Bsp1286I	GDGCHC	5 cut(s)	245, 497, 603, 706, 796
Bsp13I	TCCGGA	1 cut(s)	345
Bsp1407I	TGTACA	1 cut(s)	778
Bsp143I	GATC	5 cut(s)	61, 163, 223, 331, 874
BspANI	GGCC	9 cut(s)	5, 20, 29, 441, 455, 485, 563, 579, 955
BspEI	TCCGGA	1 cut(s)	345
BspFNI	CGCG	2 cut(s)	101, 515
BspHI	TCATGA	1 cut(s)	471
BspLI	GGNNCC	3 cut(s)	244, 416, 661
BspPI	GGATC	1 cut(s)	339
BspT107I	GGYRCC	2 cut(s)	242, 414
BsrGI	TGTACA	1 cut(s)	778
BssECI	CCNNGG	6 cut(s)	246, 262, 390, 436, 574, 722
BssMI	GATC	5 cut(s)	61, 163, 223, 331, 874
BssNI	GRCGYC	1 cut(s)	415
Bst2UI	CCWGG	5 cut(s)	247, 575, 723, 801, 899
Bst6I	CTCTTC	1 cut(s)	221
BstACI	GRCGYC	1 cut(s)	415
BstAUI	TGTACA	1 cut(s)	778
BstC8I	GCNNGC	1 cut(s)	581
BstDSI	CCRYGG	1 cut(s)	436
BstF5I	GGATG	5 cut(s)	195, 408, 615, 702, 732
BstFNI	CGCG	2 cut(s)	101, 515
BstH2I	RGCGCY	1 cut(s)	418
BstHHI	GCGC	1 cut(s)	417
BstKTI	GATC	5 cut(s)	64, 166, 226, 334, 877
BstMAI	GTCTC	1 cut(s)	94
BstMBI	GATC	5 cut(s)	61, 163, 223, 331, 874
BstMCI	CGRYCG	2 cut(s)	21, 64
BstMWI	GCNNNNNNNGC	6 cut(s)	26, 146, 412, 438, 518, 653
BstNI	CCWGG	5 cut(s)	247, 575, 723, 801, 899
BstSCI	CCNGG	9 cut(s)	109, 245, 261, 363, 476, 573, 721, 799, 897
BstSLI	GKGCMC	1 cut(s)	245
BstUI	CGCG	2 cut(s)	101, 515
BstV1I	GCAGC	1 cut(s)	643
BstX2I	RGATCY	2 cut(s)	163, 331
BstXI	CCANNNNNNTGG	1 cut(s)	328
BstYI	RGATCY	2 cut(s)	163, 331
BstZI	CGGCCG	1 cut(s)	18
BsuRI	GGCC	9 cut(s)	5, 20, 29, 441, 455, 485, 563, 579, 955
BtgI	CCRYGG	1 cut(s)	436
BtgZI	GCGATG	2 cut(s)	530, 657
BtrI	CACGTC	1 cut(s)	292
BtsCI	GGATG	5 cut(s)	195, 408, 615, 702, 732
Cac8I	GCNNGC	1 cut(s)	581
CaiI	CAGNNNCTG	1 cut(s)	580
CciI	TCATGA	1 cut(s)	471
CfoI	GCGC	1 cut(s)	417
Cfr13I	GGNCC	2 cut(s)	27, 577
CseI	GACGC	2 cut(s)	107, 155
Csp6I	GTAC	2 cut(s)	779, 827
CviAII	CATG	3 cut(s)	472, 487, 974
CviQI	GTAC	2 cut(s)	779, 827
DinI	GGCGCC	1 cut(s)	416
DpnI	GATC	5 cut(s)	63, 165, 225, 333, 876
DpnII	GATC	5 cut(s)	61, 163, 223, 331, 874
DrdI	GACNNNNNNGTC	1 cut(s)	290
DseDI	GACNNNNNNGTC	1 cut(s)	290
EaeI	YGGCCR	3 cut(s)	3, 18, 561
EagI	CGGCCG	1 cut(s)	18
Eam1104I	CTCTTC	1 cut(s)	221
EarI	CTCTTC	1 cut(s)	221
EciI	GGCGGA	1 cut(s)	842
Ecl136II	GAGCTC	2 cut(s)	495, 794
EclXI	CGGCCG	1 cut(s)	18
Eco24I	GRGCYC	4 cut(s)	497, 603, 706, 796
Eco52I	CGGCCG	1 cut(s)	18
Eco53kI	GAGCTC	2 cut(s)	495, 794
Eco88I	CYCGRG	2 cut(s)	335, 602
EcoICRI	GAGCTC	2 cut(s)	495, 794
EcoO109I	RGGNCCY	1 cut(s)	577
EcoRII	CCWGG	5 cut(s)	245, 573, 721, 799, 897
EcoT22I	ATGCAT	2 cut(s)	688, 951
EcoT38I	GRGCYC	4 cut(s)	497, 603, 706, 796
EgeI	GGCGCC	1 cut(s)	416
EheI	GGCGCC	1 cut(s)	416
Esp3I	CGTCTC	1 cut(s)	94
FaeI	CATG	3 cut(s)	475, 490, 977
FaqI	GGGAC	1 cut(s)	803
FatI	CATG	3 cut(s)	471, 486, 973
FauI	CCCGC	3 cut(s)	294, 514, 649
FbaI	TGATCA	1 cut(s)	874
Fnu4HI	GCNGC	3 cut(s)	54, 513, 632
FokI	GGATG	5 cut(s)	182, 415, 622, 709, 739
FriOI	GRGCYC	4 cut(s)	497, 603, 706, 796
Fsp4HI	GCNGC	3 cut(s)	54, 513, 632
FspBI	CTAG	1 cut(s)	834
GlaI	GCGC	1 cut(s)	416
GluI	GCNGC	3 cut(s)	54, 513, 632
GsuI	CTGGAG	1 cut(s)	915
HaeII	RGCGCY	1 cut(s)	418
HaeIII	GGCC	9 cut(s)	5, 20, 29, 441, 455, 485, 563, 579, 955
HapII	CCGG	5 cut(s)	111, 262, 346, 365, 478
HgaI	GACGC	2 cut(s)	107, 155
HhaI	GCGC	1 cut(s)	417
Hin1I	GRCGYC	1 cut(s)	415
Hin1II	CATG	3 cut(s)	475, 490, 977
Hin6I	GCGC	1 cut(s)	415
HinP1I	GCGC	1 cut(s)	415
HincII	GTYRAC	1 cut(s)	675
HindII	GTYRAC	1 cut(s)	675
HinfI	GANTC	6 cut(s)	12, 182, 299, 357, 449, 1006
HpaI	GTTAAC	1 cut(s)	675
HpaII	CCGG	5 cut(s)	111, 262, 346, 365, 478
HphI	GGTGA	6 cut(s)	64, 202, 298, 439, 489, 887
Hpy166II	GTNNAC	2 cut(s)	675, 781
Hpy188I	TCNGA	6 cut(s)	145, 356, 626, 664, 939, 1005
Hpy188III	TCNNGA	6 cut(s)	335, 337, 346, 472, 604, 886
Hpy8I	GTNNAC	2 cut(s)	675, 781
Hpy99I	CGWCG	6 cut(s)	62, 101, 149, 182, 224, 296
HpyAV	CCTTC	5 cut(s)	103, 320, 475, 892, 936
HpyCH4IV	ACGT	2 cut(s)	177, 291
HpyCH4V	TGCA	6 cut(s)	41, 406, 631, 686, 949, 963
HpyF10VI	GCNNNNNNNGC	6 cut(s)	26, 146, 412, 438, 518, 653
HpySE526I	ACGT	2 cut(s)	177, 291
Hsp92I	GRCGYC	1 cut(s)	415
Hsp92II	CATG	3 cut(s)	475, 490, 977
HspAI	GCGC	1 cut(s)	415
KasI	GGCGCC	1 cut(s)	414
Kpn2I	TCCGGA	1 cut(s)	345
Ksp22I	TGATCA	1 cut(s)	874
KspAI	GTTAAC	1 cut(s)	675
Kzo9I	GATC	5 cut(s)	61, 163, 223, 331, 874
LmnI	GCTCC	2 cut(s)	799, 940
Lsp1109I	GCAGC	1 cut(s)	643
LweI	GCATC	4 cut(s)	393, 634, 673, 930
MaeI	CTAG	1 cut(s)	834
MaeII	ACGT	2 cut(s)	177, 291
MaeIII	GTNAC	2 cut(s)	545, 635
MalI	GATC	5 cut(s)	63, 165, 225, 333, 876
MboI	GATC	5 cut(s)	61, 163, 223, 331, 874
MboII	GAAGA	2 cut(s)	208, 765
MfeI	CAATTG	1 cut(s)	200
MflI	RGATCY	2 cut(s)	163, 331
MhlI	GDGCHC	5 cut(s)	245, 497, 603, 706, 796
MlsI	TGGCCA	2 cut(s)	5, 563
MluCI	AATT	9 cut(s)	34, 200, 256, 341, 350, 554, 566, 594, 707
MluNI	TGGCCA	2 cut(s)	5, 563
Mly113I	GGCGCC	1 cut(s)	415
MlyI	GAGTC	1 cut(s)	443
MmeI	TCCRAC	1 cut(s)	168
Mox20I	TGGCCA	2 cut(s)	5, 563
Mph1103I	ATGCAT	2 cut(s)	688, 951
MroI	TCCGGA	1 cut(s)	345
MroXI	GAANNNNTTC	1 cut(s)	139
MscI	TGGCCA	2 cut(s)	5, 563
MseI	TTAA	3 cut(s)	174, 674, 752
Msp20I	TGGCCA	2 cut(s)	5, 563
MspI	CCGG	5 cut(s)	111, 262, 346, 365, 478
MspR9I	CCNGG	9 cut(s)	111, 247, 263, 365, 478, 575, 723, 801, 899
MunI	CAATTG	1 cut(s)	200
Mva1269I	GAATGC	1 cut(s)	963
MvaI	CCWGG	5 cut(s)	247, 575, 723, 801, 899
MvnI	CGCG	2 cut(s)	101, 515
MwoI	GCNNNNNNNGC	6 cut(s)	26, 146, 412, 438, 518, 653
NarI	GGCGCC	1 cut(s)	415
NciI	CCSGG	4 cut(s)	111, 263, 365, 478
NdeII	GATC	5 cut(s)	61, 163, 223, 331, 874
NlaIII	CATG	3 cut(s)	475, 490, 977
NlaIV	GGNNCC	3 cut(s)	244, 416, 661
NmeAIII	GCCGAG	4 cut(s)	207, 415, 431, 516
NmuCI	GTSAC	1 cut(s)	545
NsiI	ATGCAT	2 cut(s)	688, 951
PaeR7I	CTCGAG	2 cut(s)	335, 602
PagI	TCATGA	1 cut(s)	471
PcsI	WCGNNNNNNNCGW	3 cut(s)	57, 93, 279
PctI	GAATGC	1 cut(s)	963
PdmI	GAANNNNTTC	1 cut(s)	139
PfeI	GAWTC	5 cut(s)	12, 182, 299, 357, 1006
PfoI	TCCNGGA	1 cut(s)	109
PkrI	GCNGC	3 cut(s)	55, 514, 633
Ple19I	CGATCG	1 cut(s)	64
PleI	GAGTC	1 cut(s)	443
PluTI	GGCGCC	1 cut(s)	418
PpsI	GAGTC	1 cut(s)	443
Psp124BI	GAGCTC	2 cut(s)	497, 796
Psp6I	CCWGG	5 cut(s)	245, 573, 721, 799, 897
PspGI	CCWGG	5 cut(s)	245, 573, 721, 799, 897
PspN4I	GGNNCC	3 cut(s)	244, 416, 661
PspPI	GGNCC	2 cut(s)	27, 577
PstNI	CAGNNNCTG	1 cut(s)	580
PsuI	RGATCY	2 cut(s)	163, 331
PvuI	CGATCG	1 cut(s)	64
RsaI	GTAC	2 cut(s)	780, 828
RsaNI	GTAC	2 cut(s)	779, 827
SacI	GAGCTC	2 cut(s)	497, 796
SaqAI	TTAA	3 cut(s)	174, 674, 752
SatI	GCNGC	3 cut(s)	54, 513, 632
Sau3AI	GATC	5 cut(s)	61, 163, 223, 331, 874
Sau96I	GGNCC	2 cut(s)	27, 577
SchI	GAGTC	1 cut(s)	443
ScrFI	CCNGG	9 cut(s)	111, 247, 263, 365, 478, 575, 723, 801, 899
SduI	GDGCHC	5 cut(s)	245, 497, 603, 706, 796
SfaNI	GCATC	4 cut(s)	393, 634, 673, 930
SfiI	GGCCNNNNNGGCC	1 cut(s)	26
SfoI	GGCGCC	1 cut(s)	416
Sfr274I	CTCGAG	2 cut(s)	335, 602
SlaI	CTCGAG	2 cut(s)	335, 602
SmlI	CTYRAG	3 cut(s)	335, 602, 984
SmoI	CTYRAG	3 cut(s)	335, 602, 984
Sse9I	AATT	9 cut(s)	34, 200, 256, 341, 350, 554, 566, 594, 707
SspDI	GGCGCC	1 cut(s)	414
SspMI	CTAG	1 cut(s)	834
SstI	GAGCTC	2 cut(s)	497, 796
StyD4I	CCNGG	9 cut(s)	109, 245, 261, 363, 476, 573, 721, 799, 897
TaiI	ACGT	2 cut(s)	180, 294
TaqI	TCGA	8 cut(s)	60, 96, 180, 219, 336, 360, 375, 603
TasI	AATT	9 cut(s)	34, 200, 256, 341, 350, 554, 566, 594, 707
TatI	WGTACW	1 cut(s)	778
TauI	GCSGC	2 cut(s)	56, 515
TfiI	GAWTC	5 cut(s)	12, 182, 299, 357, 1006
Tru1I	TTAA	3 cut(s)	174, 674, 752
Tru9I	TTAA	3 cut(s)	174, 674, 752
TseFI	GTSAC	1 cut(s)	545
TseI	GCWGC	1 cut(s)	631
Tsp45I	GTSAC	1 cut(s)	545
TspDTI	ATGAA	3 cut(s)	460, 479, 962
XapI	RAATTY	4 cut(s)	341, 350, 554, 566
XcmI	CCANNNNNNNNNTGG	1 cut(s)	581
XhoI	CTCGAG	2 cut(s)	335, 602
XmnI	GAANNNNTTC	1 cut(s)	139
XspI	CTAG	1 cut(s)	834
Zsp2I	ATGCAT	2 cut(s)	688, 951

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.