RLG00000019225

CCR4-associated factor 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000757
Physical Location & Seq
Forward (+)
48623952 .. 48624809
858 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019225

Sequence Viewer

Length: 858 bp
ATGCAAGCTGTCGAAGGTACACCATGTCTGCAGCTTCCCTTACCTCCTGTGCCGGTCATAAATCCTTACTTGGGTACAATGGAGTACTGCTACCCAATTATTCCCCTTCCCATGGATGATCCCGACCCTCTTCCTATACCTGGCGTTATATATCCGGTTTGGAAGAGCAATTTAGTGCCGGCGTTTTTGCAAATTTGGAAACTAATTAGCCAAGGTGGCCGCTGGTTCGCTTCTATAGACACAGAATTCCCTGGCACAATTTATAAATCCGACATAGGCCGTCTCCATGACCCTCGATACAAGTATATGATGATGACGAAGAATGTGAACGAGACCAACATAATCCAGTTGGGTCTCACACTTGCAGACCTTAACCAAAACATCTACACCTGGGAGTTCAATTTCAGCGACTTTGATATTGACCGTGATCGTCAAGCCGAGGACTCAATTCAGTTACTTAAGGGTCAAGGAATCGATTTCTGGAGAAACAAGGAGGATGGTATACCTTCGCATGAGTTCGCGAGAATGTGTCGGGAAGTTGGGCTGTTAGAGAACAGATTGGTAGAGTTGACATGGGTTGGTTTCCAGATCTCATCTGATTTGGGCTACTTCACCAAGATCCTCACTGGGCAAAAGTTGCCAGACGATTTTGAGGAGTACAAGAAGGCTGAAAGGATCTACTTTGGGGATAGGGTGTGTGATGTCAAGGACATGATGAACTCGTTTCCGACACTAAAAGGCGGTTTGAACAACGTGGCGGAGAAACTGGGGATTGCTCGTCTGGCCGGCAAGAGTCACCAGGCCGGGTCTGATAGTCTGTTGACATATCTAACCTTCATCAAGCTGAGCTCAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1

Protein Analysis

286

Amino Acids

32.52

Weight (kDa)

5.01

Isoelectric Point (pI)

27.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 53 - 169 9.4e-09 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 264
AccI GTMKAC 1 cut(s) 502
AccII CGCG 1 cut(s) 521
AciI CCGC 3 cut(s) 220, 741, 758
AclWI GGATC 3 cut(s) 113, 613, 683
AcoI YGGCCR 2 cut(s) 217, 783
AcsI RAATTY 2 cut(s) 192, 245
AfaI GTAC 4 cut(s) 19, 76, 86, 659
AfiI CCNNNNNNNGG 3 cut(s) 71, 112, 140
AflII CTTAAG 1 cut(s) 458
AgsI TTSAA 2 cut(s) 400, 748
AjnI CCWGG 4 cut(s) 139, 250, 389, 798
AluBI AGCT 4 cut(s) 8, 34, 844, 849
AluI AGCT 4 cut(s) 8, 34, 844, 849
Alw21I GWGCWC 1 cut(s) 851
Alw26I GTCTC 3 cut(s) 287, 326, 359
AlwI GGATC 3 cut(s) 113, 613, 683
AoxI GGCC 4 cut(s) 217, 277, 783, 801
ApeKI GCWGC 1 cut(s) 31
ApoI RAATTY 2 cut(s) 192, 245
AsuC2I CCSGG 1 cut(s) 805
AsuHPI GGTGA 2 cut(s) 604, 788
BanII GRGCYC 1 cut(s) 851
Bbv12I GWGCWC 1 cut(s) 851
BbvI GCAGC 1 cut(s) 43
BccI CCATC 1 cut(s) 491
BceAI ACGGC 1 cut(s) 264
BciT130I CCWGG 4 cut(s) 141, 252, 391, 800
BcnI CCSGG 1 cut(s) 805
BcoDI GTCTC 3 cut(s) 287, 326, 359
BfmI CTRYAG 2 cut(s) 29, 234
BfrI CTTAAG 1 cut(s) 458
BglI GCCNNNNNGGC 1 cut(s) 216
BglII AGATCT 1 cut(s) 588
BisI GCNGC 2 cut(s) 32, 220
BlpI GCTNAGC 1 cut(s) 845
BlsI GCNGC 2 cut(s) 33, 221
BmcAI AGTACT 1 cut(s) 86
Bme1390I CCNGG 5 cut(s) 141, 252, 391, 800, 805
BmrFI CCNGG 5 cut(s) 141, 252, 391, 800, 805
BmrI ACTGGG 2 cut(s) 636, 776
BmuI ACTGGG 2 cut(s) 636, 776
BpmI CTGGAG 1 cut(s) 502
Bpu1102I GCTNAGC 1 cut(s) 845
BpuMI CCSGG 1 cut(s) 805
Bsa29I ATCGAT 1 cut(s) 474
BsaI GGTCTC 2 cut(s) 326, 359
BsaJI CCNNGG 5 cut(s) 111, 211, 250, 390, 438
BsaWI WCCGGW 1 cut(s) 154
Bsc4I CCNNNNNNNGG 3 cut(s) 71, 112, 140
Bse118I RCCGGY 3 cut(s) 52, 178, 785
Bse1I ACTGG 3 cut(s) 346, 631, 771
BseBI CCWGG 4 cut(s) 141, 252, 391, 800
BseCI ATCGAT 1 cut(s) 474
BseDI CCNNGG 5 cut(s) 111, 211, 250, 390, 438
BseGI GGATG 2 cut(s) 121, 502
BseLI CCNNNNNNNGG 3 cut(s) 71, 112, 140
BseMII CTCAG 1 cut(s) 836
BseNI ACTGG 3 cut(s) 346, 631, 771
BseRI GAGGAG 1 cut(s) 668
BseXI GCAGC 1 cut(s) 43
Bsh1236I CGCG 1 cut(s) 521
BshFI GGCC 4 cut(s) 219, 279, 785, 803
BshVI ATCGAT 1 cut(s) 474
BsiHKAI GWGCWC 1 cut(s) 851
BsiSI CCGG 5 cut(s) 53, 155, 179, 786, 804
BslI CCNNNNNNNGG 3 cut(s) 71, 112, 140
BsmAI GTCTC 3 cut(s) 287, 326, 359
BsmBI CGTCTC 1 cut(s) 287
BsnI GGCC 4 cut(s) 219, 279, 785, 803
Bso31I GGTCTC 2 cut(s) 326, 359
Bsp1286I GDGCHC 1 cut(s) 851
Bsp143I GATC 5 cut(s) 118, 427, 588, 618, 675
Bsp1720I GCTNAGC 1 cut(s) 845
Bsp19I CCATGG 1 cut(s) 111
Bsp68I TCGCGA 1 cut(s) 521
BspACI CCGC 3 cut(s) 220, 741, 758
BspANI GGCC 4 cut(s) 219, 279, 785, 803
BspCNI CTCAG 1 cut(s) 837
BspDI ATCGAT 1 cut(s) 474
BspFNI CGCG 1 cut(s) 521
BspMAI CTGCAG 1 cut(s) 33
BspPI GGATC 3 cut(s) 113, 613, 683
BspQI GCTCTTC 1 cut(s) 158
BspTI CTTAAG 1 cut(s) 458
BspTNI GGTCTC 2 cut(s) 326, 359
BsrFI RCCGGY 3 cut(s) 52, 178, 785
BsrI ACTGG 3 cut(s) 346, 631, 771
BssAI RCCGGY 3 cut(s) 52, 178, 785
BssECI CCNNGG 5 cut(s) 111, 211, 250, 390, 438
BssMI GATC 5 cut(s) 118, 427, 588, 618, 675
BssNAI GTATAC 1 cut(s) 503
BssT1I CCWWGG 2 cut(s) 111, 211
Bst1107I GTATAC 1 cut(s) 503
Bst2UI CCWGG 4 cut(s) 141, 252, 391, 800
Bst4CI ACNGT 1 cut(s) 425
Bst6I CTCTTC 2 cut(s) 135, 158
BstAFI CTTAAG 1 cut(s) 458
BstAPI GCANNNNNTGC 1 cut(s) 637
BstC8I GCNNGC 3 cut(s) 6, 180, 787
BstDEI CTNAG 1 cut(s) 845
BstDSI CCRYGG 1 cut(s) 111
BstF5I GGATG 2 cut(s) 121, 502
BstFNI CGCG 1 cut(s) 521
BstKTI GATC 5 cut(s) 121, 430, 591, 621, 678
BstMAI GTCTC 3 cut(s) 287, 326, 359
BstMBI GATC 5 cut(s) 118, 427, 588, 618, 675
BstMWI GCNNNNNNNGC 3 cut(s) 216, 637, 782
BstNI CCWGG 4 cut(s) 141, 252, 391, 800
BstSCI CCNGG 5 cut(s) 139, 250, 389, 798, 803
BstSFI CTRYAG 2 cut(s) 29, 234
BstUI CGCG 1 cut(s) 521
BstV1I GCAGC 1 cut(s) 43
BstX2I RGATCY 3 cut(s) 588, 618, 675
BstYI RGATCY 3 cut(s) 588, 618, 675
BstZ17I GTATAC 1 cut(s) 503
Bsu15I ATCGAT 1 cut(s) 474
BsuRI GGCC 4 cut(s) 219, 279, 785, 803
BsuTUI ATCGAT 1 cut(s) 474
BtgI CCRYGG 1 cut(s) 111
BtsCI GGATG 2 cut(s) 121, 502
BtsIMutI CAGTG 1 cut(s) 624
BtuMI TCGCGA 1 cut(s) 521
Cac8I GCNNGC 3 cut(s) 6, 180, 787
Cfr10I RCCGGY 3 cut(s) 52, 178, 785
ClaI ATCGAT 1 cut(s) 474
Csp6I GTAC 4 cut(s) 18, 75, 85, 658
CviAII CATG 6 cut(s) 24, 112, 287, 512, 573, 712
CviQI GTAC 4 cut(s) 18, 75, 85, 658
DdeI CTNAG 1 cut(s) 845
DpnI GATC 5 cut(s) 120, 429, 590, 620, 677
DpnII GATC 5 cut(s) 118, 427, 588, 618, 675
EaeI YGGCCR 2 cut(s) 217, 783
Eam1104I CTCTTC 2 cut(s) 135, 158
EarI CTCTTC 2 cut(s) 135, 158
EciI GGCGGA 1 cut(s) 773
Ecl136II GAGCTC 1 cut(s) 849
Eco130I CCWWGG 2 cut(s) 111, 211
Eco24I GRGCYC 1 cut(s) 851
Eco31I GGTCTC 2 cut(s) 326, 359
Eco53kI GAGCTC 1 cut(s) 849
EcoICRI GAGCTC 1 cut(s) 849
EcoRI GAATTC 1 cut(s) 245
EcoRII CCWGG 4 cut(s) 139, 250, 389, 798
EcoT14I CCWWGG 2 cut(s) 111, 211
EcoT38I GRGCYC 1 cut(s) 851
ErhI CCWWGG 2 cut(s) 111, 211
Esp3I CGTCTC 1 cut(s) 287
FaeI CATG 6 cut(s) 27, 115, 290, 515, 576, 715
FatI CATG 6 cut(s) 23, 111, 286, 511, 572, 711
FblI GTMKAC 1 cut(s) 502
Fnu4HI GCNGC 2 cut(s) 32, 220
FokI GGATG 2 cut(s) 128, 509
FriOI GRGCYC 1 cut(s) 851
Fsp4HI GCNGC 2 cut(s) 32, 220
GluI GCNGC 2 cut(s) 32, 220
GsuI CTGGAG 1 cut(s) 502
HaeIII GGCC 4 cut(s) 219, 279, 785, 803
HapII CCGG 5 cut(s) 53, 155, 179, 786, 804
Hin1II CATG 6 cut(s) 27, 115, 290, 515, 576, 715
HincII GTYRAC 2 cut(s) 570, 822
HindII GTYRAC 2 cut(s) 570, 822
HinfI GANTC 3 cut(s) 443, 471, 793
HpaII CCGG 5 cut(s) 53, 155, 179, 786, 804
HphI GGTGA 2 cut(s) 604, 788
Hpy166II GTNNAC 5 cut(s) 20, 328, 503, 570, 822
Hpy188I TCNGA 4 cut(s) 271, 598, 729, 811
Hpy188III TCNNGA 5 cut(s) 122, 481, 520, 533, 586
Hpy8I GTNNAC 5 cut(s) 20, 328, 503, 570, 822
HpyAV CCTTC 5 cut(s) 8, 116, 516, 658, 844
HpyCH4III ACNGT 1 cut(s) 425
HpyCH4IV ACGT 1 cut(s) 753
HpyCH4V TGCA 4 cut(s) 4, 31, 190, 365
HpyF10VI GCNNNNNNNGC 3 cut(s) 216, 637, 782
HpyF3I CTNAG 1 cut(s) 845
HpySE526I ACGT 1 cut(s) 753
Hsp92II CATG 6 cut(s) 27, 115, 290, 515, 576, 715
KroI GCCGGC 2 cut(s) 178, 785
KroNI GCCGGC 2 cut(s) 180, 787
Kzo9I GATC 5 cut(s) 118, 427, 588, 618, 675
LguI GCTCTTC 1 cut(s) 158
Lsp1109I GCAGC 1 cut(s) 43
MaeII ACGT 1 cut(s) 753
MaeIII GTNAC 2 cut(s) 453, 794
MalI GATC 5 cut(s) 120, 429, 590, 620, 677
MboI GATC 5 cut(s) 118, 427, 588, 618, 675
MboII GAAGA 3 cut(s) 122, 175, 331
MflI RGATCY 3 cut(s) 588, 618, 675
MhlI GDGCHC 1 cut(s) 851
MluCI AATT 8 cut(s) 96, 169, 192, 204, 245, 258, 400, 447
MlyI GAGTC 2 cut(s) 437, 802
MmeI TCCRAC 2 cut(s) 294, 752
MnlI CCTC 7 cut(s) 54, 138, 303, 433, 487, 632, 646
MroNI GCCGGC 2 cut(s) 178, 785
MseI TTAA 2 cut(s) 372, 459
MspA1I CMGCKG 1 cut(s) 222
MspCI CTTAAG 1 cut(s) 458
MspI CCGG 5 cut(s) 53, 155, 179, 786, 804
MspR9I CCNGG 5 cut(s) 141, 252, 391, 800, 805
MvaI CCWGG 4 cut(s) 141, 252, 391, 800
MvnI CGCG 1 cut(s) 521
MwoI GCNNNNNNNGC 3 cut(s) 216, 637, 782
NaeI GCCGGC 2 cut(s) 180, 787
NciI CCSGG 1 cut(s) 805
NcoI CCATGG 1 cut(s) 111
NdeII GATC 5 cut(s) 118, 427, 588, 618, 675
NgoMIV GCCGGC 2 cut(s) 178, 785
NlaIII CATG 6 cut(s) 27, 115, 290, 515, 576, 715
NmeAIII GCCGAG 1 cut(s) 463
NmuCI GTSAC 1 cut(s) 794
NruI TCGCGA 1 cut(s) 521
PciSI GCTCTTC 1 cut(s) 158
PdiI GCCGGC 2 cut(s) 180, 787
PfeI GAWTC 1 cut(s) 471
PkrI GCNGC 2 cut(s) 33, 221
PleI GAGTC 2 cut(s) 437, 801
PpsI GAGTC 2 cut(s) 437, 801
PsiI TTATAA 1 cut(s) 264
Psp124BI GAGCTC 1 cut(s) 851
Psp6I CCWGG 4 cut(s) 139, 250, 389, 798
PspGI CCWGG 4 cut(s) 139, 250, 389, 798
PstI CTGCAG 1 cut(s) 33
PsuI RGATCY 3 cut(s) 588, 618, 675
RruI TCGCGA 1 cut(s) 521
RsaI GTAC 4 cut(s) 19, 76, 86, 659
RsaNI GTAC 4 cut(s) 18, 75, 85, 658
SacI GAGCTC 1 cut(s) 851
SapI GCTCTTC 1 cut(s) 158
SaqAI TTAA 2 cut(s) 372, 459
SatI GCNGC 2 cut(s) 32, 220
Sau3AI GATC 5 cut(s) 118, 427, 588, 618, 675
ScaI AGTACT 1 cut(s) 86
SchI GAGTC 2 cut(s) 437, 802
ScrFI CCNGG 5 cut(s) 141, 252, 391, 800, 805
SduI GDGCHC 1 cut(s) 851
SfcI CTRYAG 2 cut(s) 29, 234
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
Sse9I AATT 8 cut(s) 96, 169, 192, 204, 245, 258, 400, 447
SsiI CCGC 3 cut(s) 220, 741, 758
SstI GAGCTC 1 cut(s) 851
StyD4I CCNGG 5 cut(s) 139, 250, 389, 798, 803
StyI CCWWGG 2 cut(s) 111, 211
TaaI ACNGT 1 cut(s) 425
TaiI ACGT 1 cut(s) 756
TaqI TCGA 3 cut(s) 12, 295, 474
TasI AATT 8 cut(s) 96, 169, 192, 204, 245, 258, 400, 447
TatI WGTACW 2 cut(s) 84, 657
TauI GCSGC 1 cut(s) 222
TfiI GAWTC 1 cut(s) 471
Tru1I TTAA 2 cut(s) 372, 459
Tru9I TTAA 2 cut(s) 372, 459
TscAI CASTG 1 cut(s) 631
TseFI GTSAC 1 cut(s) 794
TseI GCWGC 1 cut(s) 31
Tsp45I GTSAC 1 cut(s) 794
TspDTI ATGAA 2 cut(s) 731, 826
TspRI CASTG 1 cut(s) 631
Vha464I CTTAAG 1 cut(s) 458
XapI RAATTY 2 cut(s) 192, 245
XmiI GTMKAC 1 cut(s) 502
ZrmI AGTACT 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.