RchiOBHm_Chr2g0136851

CCR4-associated factor 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000757
Physical Location & Seq
Reverse (-)
54166156 .. 54167214
1059 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50763

Sequence Viewer

Length: 1059 bp
ATGATGATAGTTCAGAGAGGAATTCCGATCTTCCCAATTGGGCCTGCTTCTGATTTCCGCGGCGGAGTTCCGGTGGTCTTCCACCGCCGACCTGTGCCTCTGATTCCTCTACACCACCGCCCTATACCCCTTTACCCTCCTCAATTTCAGAGCCGCGTCCCTGTTTTCAGCGTTGTCGGCGCCCCAATTTGTCGTCAAGGATCTGTGGAGAAAGCCGATCGGTTCGAGGTTCGAAGTGTTTGTCGCTATAATTACAAAGCCGAGATTGCCGTAATTCAGAGTCTAATCAGGGCCGGCTACCGATTTGCTAATTTCGACACCGAATTCCCCGGGACAGTGGTTAAATCCGAAATTCCGAAGCATCTGATATCTCAAGCTGTTCCGGTTGATGTGTATAAGATGATGAAGACCAATGTGGACCAAACCCATATTATCCAATTGGGTCTTTCACTCTCTGACCCATTTGGGAATTTGCCGGTTTATGATGGTGCTTACTGTTGTTGGGAGTTTAATTTCAGTGATTTTGATGTGGACTGTACTCAACATTTGAGGAATACTGTCTCGATTGATGTCCTGAAGCGGCAGGGAATAGACTTTTCCGACAACAAATGCACTGGTATTTGTTCTGCAGACTTTGCTGAGGAGATTGAGAGGTCTGGGCTAGTGGAGTTGCTTCCTTGCTTGACTTGGGCTACTTTCCAGAGTGCATATGATTTTGGGTACTTGATAAAGATGTTCACTGGGAACAAGGAACTGCCAGAGGATATCAAGGAGTTCATGGGCAACGTGAAGACATATTTCGGACCGAATGTGTACGACATCAAGTACATGATGAAGTTCTGTGATGGACTGTTTGGCGGTTTTAATAGCGTGGCAGAAACCCTTGGCGTTGACCGTGTGGCTGGGAGTAGCCACCAGGCTGGTTCTGATAGTCTTTTGACACTGCAAACGTTTGTCAAGATGCTCAAGATGGAGAAGAACAAAGGGAAAGATCTCTGGGAAGAATGTGGAGGAGTTTTGTTTGGTTTAGGAAGCTTGGATTTCTGGCATCATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1 RVT_3

Protein Analysis

352

Amino Acids

39.59

Weight (kDa)

6.15

Isoelectric Point (pI)

46.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 81 - 208 1.5e-06 CAF1 family ribonuclease
CAF1 PF04857 236 - 279 3.5e-06 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 179
AccII CGCG 2 cut(s) 60, 156
AciI CCGC 8 cut(s) 58, 60, 63, 85, 118, 154, 580, 858
AclI AACGTT 1 cut(s) 950
AclWI GGATC 1 cut(s) 208
AcsI RAATTY 4 cut(s) 21, 323, 351, 469
AcuI CTGAAG 1 cut(s) 596
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 4 cut(s) 538, 722, 815, 827
AjnI CCWGG 1 cut(s) 915
AluBI AGCT 2 cut(s) 377, 1035
AluI AGCT 2 cut(s) 377, 1035
Alw26I GTCTC 1 cut(s) 565
AlwI GGATC 1 cut(s) 208
Ama87I CYCGRG 1 cut(s) 329
AoxI GGCC 2 cut(s) 41, 291
ApoI RAATTY 4 cut(s) 21, 323, 351, 469
ArsI GACNNNNNNTTYG 2 cut(s) 226, 258
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 4 cut(s) 41, 291, 418, 803
AsuC2I CCSGG 2 cut(s) 330, 331
AsuII TTCGAA 1 cut(s) 232
AvaI CYCGRG 1 cut(s) 329
AvaII GGWCC 2 cut(s) 418, 803
BanI GGYRCC 1 cut(s) 179
BbsI GAAGAC 3 cut(s) 70, 413, 797
BbvCI CCTCAGC 1 cut(s) 639
BccI CCATC 3 cut(s) 479, 839, 964
BceAI ACGGC 1 cut(s) 254
BciT130I CCWGG 1 cut(s) 917
BcnI CCSGG 2 cut(s) 330, 331
BcoDI GTCTC 1 cut(s) 565
BfaI CTAG 1 cut(s) 662
BfmI CTRYAG 1 cut(s) 627
BfoI RGCGCY 1 cut(s) 183
BglII AGATCT 1 cut(s) 991
BisI GCNGC 3 cut(s) 61, 154, 581
BlsI GCNGC 3 cut(s) 62, 155, 582
Bme1390I CCNGG 3 cut(s) 330, 331, 917
Bme18I GGWCC 2 cut(s) 418, 803
BmeT110I CYCGRG 1 cut(s) 329
BmgT120I GGNCC 4 cut(s) 41, 291, 418, 803
BmiI GGNNCC 1 cut(s) 181
BmrFI CCNGG 3 cut(s) 330, 331, 917
BmrI ACTGGG 1 cut(s) 750
BmsI GCATC 2 cut(s) 370, 951
BmuI ACTGGG 1 cut(s) 750
BpiI GAAGAC 3 cut(s) 70, 413, 797
Bpu10I CCTNAGC 1 cut(s) 639
Bpu14I TTCGAA 1 cut(s) 232
BpuEI CTTGAG 2 cut(s) 357, 950
BpuMI CCSGG 2 cut(s) 330, 331
BsaHI GRCGYC 1 cut(s) 180
BsaJI CCNNGG 4 cut(s) 58, 328, 329, 883
BsaWI WCCGGW 2 cut(s) 70, 382
BsaXI ACNNNNNCTCC 2 cut(s) 57, 87
Bse118I RCCGGY 2 cut(s) 293, 475
Bse1I ACTGG 2 cut(s) 619, 745
BseBI CCWGG 1 cut(s) 917
BseDI CCNNGG 4 cut(s) 58, 328, 329, 883
BseMII CTCAG 1 cut(s) 630
BseNI ACTGG 2 cut(s) 619, 745
BseRI GAGGAG 3 cut(s) 129, 656, 1026
BseYI CCCAGC 1 cut(s) 902
Bsh1236I CGCG 2 cut(s) 60, 156
Bsh1285I CGRYCG 1 cut(s) 220
BshFI GGCC 2 cut(s) 43, 293
BshNI GGYRCC 1 cut(s) 179
BsiEI CGRYCG 1 cut(s) 220
BsiHKCI CYCGRG 1 cut(s) 329
BsiSI CCGG 5 cut(s) 71, 294, 330, 383, 476
BslFI GGGAC 2 cut(s) 143, 346
BsmAI GTCTC 1 cut(s) 565
BsmFI GGGAC 2 cut(s) 143, 346
BsnI GGCC 2 cut(s) 43, 293
BsoBI CYCGRG 1 cut(s) 329
Bsp119I TTCGAA 1 cut(s) 232
Bsp143I GATC 4 cut(s) 27, 200, 217, 991
BspACI CCGC 8 cut(s) 58, 60, 63, 85, 118, 154, 580, 858
BspANI GGCC 2 cut(s) 43, 293
BspCNI CTCAG 1 cut(s) 631
BspFNI CGCG 2 cut(s) 60, 156
BspLI GGNNCC 1 cut(s) 181
BspMAI CTGCAG 1 cut(s) 631
BspPI GGATC 1 cut(s) 208
BspT104I TTCGAA 1 cut(s) 232
BspT107I GGYRCC 1 cut(s) 179
BsrFI RCCGGY 2 cut(s) 293, 475
BsrI ACTGG 2 cut(s) 619, 745
BssAI RCCGGY 2 cut(s) 293, 475
BssECI CCNNGG 4 cut(s) 58, 328, 329, 883
BssMI GATC 4 cut(s) 27, 200, 217, 991
BssNI GRCGYC 1 cut(s) 180
BssT1I CCWWGG 1 cut(s) 883
Bst2UI CCWGG 1 cut(s) 917
Bst4CI ACNGT 6 cut(s) 337, 497, 536, 559, 852, 896
BstACI GRCGYC 1 cut(s) 180
BstAPI GCANNNNNTGC 1 cut(s) 635
BstBI TTCGAA 1 cut(s) 232
BstC8I GCNNGC 2 cut(s) 45, 295
BstDEI CTNAG 1 cut(s) 639
BstDSI CCRYGG 1 cut(s) 58
BstFNI CGCG 2 cut(s) 60, 156
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 4 cut(s) 30, 203, 220, 994
BstMAI GTCTC 1 cut(s) 565
BstMBI GATC 4 cut(s) 27, 200, 217, 991
BstMCI CGRYCG 1 cut(s) 220
BstMWI GCNNNNNNNGC 3 cut(s) 177, 266, 635
BstNI CCWGG 1 cut(s) 917
BstSCI CCNGG 3 cut(s) 328, 329, 915
BstSFI CTRYAG 1 cut(s) 627
BstUI CGCG 2 cut(s) 60, 156
BstV2I GAAGAC 3 cut(s) 70, 413, 797
BstX2I RGATCY 2 cut(s) 200, 991
BstXI CCANNNNNNTGG 1 cut(s) 920
BstYI RGATCY 2 cut(s) 200, 991
BsuRI GGCC 2 cut(s) 43, 293
BtgI CCRYGG 1 cut(s) 58
BtsI GCAGTG 1 cut(s) 941
BtsIMutI CAGTG 5 cut(s) 342, 523, 612, 738, 941
Cac8I GCNNGC 2 cut(s) 45, 295
CfoI GCGC 1 cut(s) 182
Cfr10I RCCGGY 2 cut(s) 293, 475
Cfr13I GGNCC 4 cut(s) 41, 291, 418, 803
Cfr42I CCGCGG 1 cut(s) 61
Cfr9I CCCGGG 1 cut(s) 329
CpoI CGGWCCG 1 cut(s) 803
CseI GACGC 1 cut(s) 145
Csp6I GTAC 4 cut(s) 537, 721, 814, 826
CspI CGGWCCG 1 cut(s) 803
CviAII CATG 2 cut(s) 778, 829
CviQI GTAC 4 cut(s) 537, 721, 814, 826
DdeI CTNAG 1 cut(s) 639
DinI GGCGCC 1 cut(s) 181
DpnI GATC 4 cut(s) 29, 202, 219, 993
DpnII GATC 4 cut(s) 27, 200, 217, 991
EciI GGCGGA 1 cut(s) 78
Eco130I CCWWGG 1 cut(s) 883
Eco32I GATATC 2 cut(s) 369, 766
Eco47I GGWCC 2 cut(s) 418, 803
Eco57I CTGAAG 1 cut(s) 596
Eco88I CYCGRG 1 cut(s) 329
EcoRI GAATTC 2 cut(s) 21, 323
EcoRII CCWGG 1 cut(s) 915
EcoRV GATATC 2 cut(s) 369, 766
EcoT14I CCWWGG 1 cut(s) 883
EgeI GGCGCC 1 cut(s) 181
EheI GGCGCC 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 883
FaeI CATG 2 cut(s) 781, 832
FaqI GGGAC 2 cut(s) 143, 346
FatI CATG 2 cut(s) 777, 828
FauNDI CATATG 1 cut(s) 709
Fnu4HI GCNGC 3 cut(s) 61, 154, 581
Fsp4HI GCNGC 3 cut(s) 61, 154, 581
FspBI CTAG 1 cut(s) 662
GlaI GCGC 1 cut(s) 181
GluI GCNGC 3 cut(s) 61, 154, 581
GsaI CCCAGC 1 cut(s) 906
HaeII RGCGCY 1 cut(s) 183
HaeIII GGCC 2 cut(s) 43, 293
HapII CCGG 5 cut(s) 71, 294, 330, 383, 476
HgaI GACGC 1 cut(s) 145
HhaI GCGC 1 cut(s) 182
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 2 cut(s) 781, 832
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HincII GTYRAC 1 cut(s) 892
HindII GTYRAC 1 cut(s) 892
HindIII AAGCTT 1 cut(s) 1033
HinfI GANTC 2 cut(s) 103, 280
HpaII CCGG 5 cut(s) 71, 294, 330, 383, 476
Hpy166II GTNNAC 5 cut(s) 418, 532, 738, 814, 892
Hpy188III TCNNGA 5 cut(s) 562, 574, 700, 958, 967
Hpy8I GTNNAC 5 cut(s) 418, 532, 738, 814, 892
HpyCH4III ACNGT 6 cut(s) 337, 497, 536, 559, 852, 896
HpyCH4IV ACGT 2 cut(s) 786, 950
HpyCH4V TGCA 4 cut(s) 612, 629, 707, 946
HpyF10VI GCNNNNNNNGC 3 cut(s) 177, 266, 635
HpyF3I CTNAG 1 cut(s) 639
HpySE526I ACGT 2 cut(s) 786, 950
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 2 cut(s) 781, 832
HspAI GCGC 1 cut(s) 180
KasI GGCGCC 1 cut(s) 179
KroI GCCGGC 1 cut(s) 293
KroNI GCCGGC 1 cut(s) 295
KspI CCGCGG 1 cut(s) 61
Kzo9I GATC 4 cut(s) 27, 200, 217, 991
LweI GCATC 2 cut(s) 370, 951
MaeI CTAG 1 cut(s) 662
MaeII ACGT 2 cut(s) 786, 950
MalI GATC 4 cut(s) 29, 202, 219, 993
MboI GATC 4 cut(s) 27, 200, 217, 991
MboII GAAGA 6 cut(s) 22, 70, 418, 802, 988, 1013
MfeI CAATTG 2 cut(s) 36, 437
MflI RGATCY 2 cut(s) 200, 991
Mly113I GGCGCC 1 cut(s) 180
MlyI GAGTC 1 cut(s) 289
MmeI TCCRAC 1 cut(s) 624
MroNI GCCGGC 1 cut(s) 293
MseI TTAA 3 cut(s) 342, 510, 864
MspA1I CMGCKG 1 cut(s) 60
MspI CCGG 5 cut(s) 71, 294, 330, 383, 476
MspR9I CCNGG 3 cut(s) 330, 331, 917
MunI CAATTG 2 cut(s) 36, 437
MvaI CCWGG 1 cut(s) 917
MvnI CGCG 2 cut(s) 60, 156
MwoI GCNNNNNNNGC 3 cut(s) 177, 266, 635
NaeI GCCGGC 1 cut(s) 295
NarI GGCGCC 1 cut(s) 180
NciI CCSGG 2 cut(s) 330, 331
NdeI CATATG 1 cut(s) 709
NdeII GATC 4 cut(s) 27, 200, 217, 991
NgoMIV GCCGGC 1 cut(s) 293
NlaIII CATG 2 cut(s) 781, 832
NlaIV GGNNCC 1 cut(s) 181
NmeAIII GCCGAG 1 cut(s) 286
NspV TTCGAA 1 cut(s) 232
PdiI GCCGGC 1 cut(s) 295
PfeI GAWTC 1 cut(s) 103
PkrI GCNGC 3 cut(s) 62, 155, 582
Ple19I CGATCG 1 cut(s) 220
PleI GAGTC 1 cut(s) 288
PluTI GGCGCC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 288
Psp1406I AACGTT 1 cut(s) 950
Psp6I CCWGG 1 cut(s) 915
PspFI CCCAGC 1 cut(s) 902
PspGI CCWGG 1 cut(s) 915
PspN4I GGNNCC 1 cut(s) 181
PspPI GGNCC 4 cut(s) 41, 291, 418, 803
PstI CTGCAG 1 cut(s) 631
PsuI RGATCY 2 cut(s) 200, 991
PvuI CGATCG 1 cut(s) 220
RsaI GTAC 4 cut(s) 538, 722, 815, 827
RsaNI GTAC 4 cut(s) 537, 721, 814, 826
Rsr2I CGGWCCG 1 cut(s) 803
RsrII CGGWCCG 1 cut(s) 803
SacII CCGCGG 1 cut(s) 61
SaqAI TTAA 3 cut(s) 342, 510, 864
SatI GCNGC 3 cut(s) 61, 154, 581
Sau3AI GATC 4 cut(s) 27, 200, 217, 991
Sau96I GGNCC 4 cut(s) 41, 291, 418, 803
SchI GAGTC 1 cut(s) 289
ScrFI CCNGG 3 cut(s) 330, 331, 917
SetI ASST 7 cut(s) 94, 231, 379, 656, 789, 953, 1037
SfaNI GCATC 2 cut(s) 370, 951
SfcI CTRYAG 1 cut(s) 627
SfoI GGCGCC 1 cut(s) 181
Sfr303I CCGCGG 1 cut(s) 61
SfuI TTCGAA 1 cut(s) 232
SgrBI CCGCGG 1 cut(s) 61
SinI GGWCC 2 cut(s) 418, 803
SmaI CCCGGG 1 cut(s) 331
SmlI CTYRAG 2 cut(s) 372, 965
SmoI CTYRAG 2 cut(s) 372, 965
SsiI CCGC 8 cut(s) 58, 60, 63, 85, 118, 154, 580, 858
SspDI GGCGCC 1 cut(s) 179
SspMI CTAG 1 cut(s) 662
StyD4I CCNGG 3 cut(s) 328, 329, 915
StyI CCWWGG 1 cut(s) 883
TaaI ACNGT 6 cut(s) 337, 497, 536, 559, 852, 896
TaiI ACGT 2 cut(s) 789, 953
TaqI TCGA 4 cut(s) 225, 232, 315, 563
TaqII GACCGA 1 cut(s) 820
TatI WGTACW 2 cut(s) 536, 825
TauI GCSGC 3 cut(s) 63, 156, 583
TfiI GAWTC 1 cut(s) 103
Tru1I TTAA 3 cut(s) 342, 510, 864
Tru9I TTAA 3 cut(s) 342, 510, 864
TscAI CASTG 5 cut(s) 342, 523, 619, 745, 948
TspDTI ATGAA 3 cut(s) 419, 766, 848
TspMI CCCGGG 1 cut(s) 329
TspRI CASTG 5 cut(s) 342, 523, 619, 745, 948
VpaK11BI GGWCC 2 cut(s) 418, 803
XapI RAATTY 4 cut(s) 21, 323, 351, 469
XmaI CCCGGG 1 cut(s) 329
XspI CTAG 1 cut(s) 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.