Rh6CG159200

CCR4-associated factor 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000757
Physical Location & Seq
Forward (+)
20671683 .. 20674931
3249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG159200.1

Sequence Viewer

Length: 792 bp
ATGGCTTCTAAGAGTCTAATCAGGGCCGGCTACCGATTTGCTAGTTTCGACACCGAATTCCCCGGGACAGTGGTTAAATCCGAAATTCCGAAACATCTGATATCTCAAGCTGTTCCGGTTGATGTGTATAAGATGATGAAGACCAATGTGGACCAAACCCATATTATCCAATTGGGTCTTTCACTCTCTGACCCATTTGGGAATTTGCCGGTTTATGATGGTGCTTACTGTTGTTGGGAGTTTAATTTCAGTGATTTTGATGTGGACTGTGCTCAACATTTGAGGAATAGTGTCTCGATTGATGTCCTGAAGCGGCAGGGAATAGACTTTTCCGACAACAAATGCACTGGTATTTGTTCTGCAGACTTTGCTGAGGAGATTGAGAGGTCTGGGTTAGTGGAGTTGCTTCCTTGCGTGACTTGGGCTACCTTCCAGAGTGCATATGATTTTGGGTACTTGATAAAGATGTTCATTGGGAACAAGGAATTTCCAGAGGATATCAAGGAGTTCATGGGCAATGTGAAGACATATTTTGGACCGAATGTGTACGACATCAAGTACATGATCAAGTTCTGTGATGGACTGTTTGGCGGTTTGAATAGCGTGGCAGCAACCCTTGGTGTTGACTGTGTGGCTGGGAGTAGCCACCAGGCTGGTTCTGATAGTCTTTTGACACTGCAAACGTTTATCAAGATGCTCAAGATGGAGAAGAACAAAGGGAAAGAGCTCTGGGAAGAATGTGGAGGAGTTTTGTTTGGTTTAGGAAGCTTGGATTTCTGGCATCATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1 RVT_3

Protein Analysis

263

Amino Acids

29.35

Weight (kDa)

5.04

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 11 - 119 2.7e-06 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 313, 591
AclI AACGTT 1 cut(s) 683
AcsI RAATTY 4 cut(s) 56, 84, 202, 485
AcuI CTGAAG 1 cut(s) 329
AfaI GTAC 3 cut(s) 455, 548, 560
AgsI TTSAA 1 cut(s) 598
AjnI CCWGG 1 cut(s) 648
AluBI AGCT 3 cut(s) 110, 727, 768
AluI AGCT 3 cut(s) 110, 727, 768
Alw21I GWGCWC 2 cut(s) 274, 729
Alw26I GTCTC 1 cut(s) 298
Ama87I CYCGRG 1 cut(s) 62
AoxI GGCC 1 cut(s) 24
ApeKI GCWGC 1 cut(s) 608
ApoI RAATTY 4 cut(s) 56, 84, 202, 485
AspS9I GGNCC 3 cut(s) 24, 151, 536
AsuC2I CCSGG 2 cut(s) 63, 64
AvaI CYCGRG 1 cut(s) 62
AvaII GGWCC 2 cut(s) 151, 536
BanII GRGCYC 1 cut(s) 729
BbsI GAAGAC 2 cut(s) 146, 530
Bbv12I GWGCWC 2 cut(s) 274, 729
BbvCI CCTCAGC 1 cut(s) 372
BbvI GCAGC 1 cut(s) 620
BccI CCATC 3 cut(s) 212, 572, 697
BciT130I CCWGG 1 cut(s) 650
BclI TGATCA 1 cut(s) 564
BcnI CCSGG 2 cut(s) 63, 64
BcoDI GTCTC 1 cut(s) 298
BfaI CTAG 1 cut(s) 42
BfmI CTRYAG 1 cut(s) 360
BisI GCNGC 2 cut(s) 314, 609
BlsI GCNGC 2 cut(s) 315, 610
Bme1390I CCNGG 3 cut(s) 63, 64, 650
Bme18I GGWCC 2 cut(s) 151, 536
BmeT110I CYCGRG 1 cut(s) 62
BmgT120I GGNCC 3 cut(s) 24, 151, 536
BmrFI CCNGG 3 cut(s) 63, 64, 650
BmsI GCATC 1 cut(s) 684
BpiI GAAGAC 2 cut(s) 146, 530
Bpu10I CCTNAGC 1 cut(s) 372
BpuEI CTTGAG 2 cut(s) 90, 683
BpuMI CCSGG 2 cut(s) 63, 64
BsaJI CCNNGG 3 cut(s) 61, 62, 616
BsaWI WCCGGW 1 cut(s) 115
Bse118I RCCGGY 2 cut(s) 26, 208
Bse1I ACTGG 1 cut(s) 352
Bse3DI GCAATG 1 cut(s) 523
BseBI CCWGG 1 cut(s) 650
BseDI CCNNGG 3 cut(s) 61, 62, 616
BseMI GCAATG 1 cut(s) 523
BseMII CTCAG 1 cut(s) 363
BseNI ACTGG 1 cut(s) 352
BseRI GAGGAG 2 cut(s) 389, 759
BseXI GCAGC 1 cut(s) 620
BseYI CCCAGC 1 cut(s) 635
BshFI GGCC 1 cut(s) 26
BsiHKAI GWGCWC 2 cut(s) 274, 729
BsiHKCI CYCGRG 1 cut(s) 62
BsiSI CCGG 4 cut(s) 27, 63, 116, 209
BslFI GGGAC 1 cut(s) 79
BsmAI GTCTC 1 cut(s) 298
BsmFI GGGAC 1 cut(s) 79
BsnI GGCC 1 cut(s) 26
BsoBI CYCGRG 1 cut(s) 62
Bsp1286I GDGCHC 2 cut(s) 274, 729
Bsp143I GATC 1 cut(s) 564
BspACI CCGC 2 cut(s) 313, 591
BspANI GGCC 1 cut(s) 26
BspCNI CTCAG 1 cut(s) 364
BspMAI CTGCAG 1 cut(s) 364
BsrDI GCAATG 1 cut(s) 523
BsrFI RCCGGY 2 cut(s) 26, 208
BsrI ACTGG 1 cut(s) 352
BssAI RCCGGY 2 cut(s) 26, 208
BssECI CCNNGG 3 cut(s) 61, 62, 616
BssMI GATC 1 cut(s) 564
BssT1I CCWWGG 1 cut(s) 616
Bst2UI CCWGG 1 cut(s) 650
Bst4CI ACNGT 5 cut(s) 70, 230, 269, 585, 629
BstAPI GCANNNNNTGC 1 cut(s) 368
BstC8I GCNNGC 1 cut(s) 28
BstDEI CTNAG 2 cut(s) 9, 372
BstKTI GATC 1 cut(s) 567
BstMAI GTCTC 1 cut(s) 298
BstMBI GATC 1 cut(s) 564
BstMWI GCNNNNNNNGC 1 cut(s) 368
BstNI CCWGG 1 cut(s) 650
BstSCI CCNGG 3 cut(s) 61, 62, 648
BstSFI CTRYAG 1 cut(s) 360
BstV1I GCAGC 1 cut(s) 620
BstV2I GAAGAC 2 cut(s) 146, 530
BstXI CCANNNNNNTGG 1 cut(s) 653
BsuRI GGCC 1 cut(s) 26
BtsI GCAGTG 1 cut(s) 674
BtsIMutI CAGTG 4 cut(s) 75, 256, 345, 674
Cac8I GCNNGC 1 cut(s) 28
Cfr10I RCCGGY 2 cut(s) 26, 208
Cfr13I GGNCC 3 cut(s) 24, 151, 536
Cfr9I CCCGGG 1 cut(s) 62
Csp6I GTAC 3 cut(s) 454, 547, 559
CviAII CATG 2 cut(s) 511, 562
CviQI GTAC 3 cut(s) 454, 547, 559
DdeI CTNAG 2 cut(s) 9, 372
DpnI GATC 1 cut(s) 566
DpnII GATC 1 cut(s) 564
Ecl136II GAGCTC 1 cut(s) 727
Eco130I CCWWGG 1 cut(s) 616
Eco24I GRGCYC 1 cut(s) 729
Eco32I GATATC 2 cut(s) 102, 499
Eco47I GGWCC 2 cut(s) 151, 536
Eco53kI GAGCTC 1 cut(s) 727
Eco57I CTGAAG 1 cut(s) 329
Eco88I CYCGRG 1 cut(s) 62
EcoICRI GAGCTC 1 cut(s) 727
EcoRI GAATTC 1 cut(s) 56
EcoRII CCWGG 1 cut(s) 648
EcoRV GATATC 2 cut(s) 102, 499
EcoT14I CCWWGG 1 cut(s) 616
EcoT38I GRGCYC 1 cut(s) 729
ErhI CCWWGG 1 cut(s) 616
FaeI CATG 2 cut(s) 514, 565
FaiI YATR 8 cut(s) 129, 162, 216, 442, 444, 512, 529, 563
FaqI GGGAC 1 cut(s) 79
FatI CATG 2 cut(s) 510, 561
FauNDI CATATG 1 cut(s) 442
FbaI TGATCA 1 cut(s) 564
Fnu4HI GCNGC 2 cut(s) 314, 609
FriOI GRGCYC 1 cut(s) 729
Fsp4HI GCNGC 2 cut(s) 314, 609
FspBI CTAG 1 cut(s) 42
GluI GCNGC 2 cut(s) 314, 609
GsaI CCCAGC 1 cut(s) 639
HaeIII GGCC 1 cut(s) 26
HapII CCGG 4 cut(s) 27, 63, 116, 209
Hin1II CATG 2 cut(s) 514, 565
HincII GTYRAC 1 cut(s) 625
HindII GTYRAC 1 cut(s) 625
HindIII AAGCTT 1 cut(s) 766
HinfI GANTC 1 cut(s) 13
HpaII CCGG 4 cut(s) 27, 63, 116, 209
Hpy166II GTNNAC 4 cut(s) 151, 265, 547, 625
Hpy188I TCNGA 6 cut(s) 82, 90, 99, 190, 334, 661
Hpy188III TCNNGA 6 cut(s) 295, 307, 433, 491, 691, 700
Hpy8I GTNNAC 4 cut(s) 151, 265, 547, 625
HpyAV CCTTC 1 cut(s) 439
HpyCH4III ACNGT 5 cut(s) 70, 230, 269, 585, 629
HpyCH4IV ACGT 1 cut(s) 683
HpyCH4V TGCA 4 cut(s) 345, 362, 440, 679
HpyF10VI GCNNNNNNNGC 1 cut(s) 368
HpyF3I CTNAG 2 cut(s) 9, 372
HpySE526I ACGT 1 cut(s) 683
Hsp92II CATG 2 cut(s) 514, 565
KroI GCCGGC 1 cut(s) 26
KroNI GCCGGC 1 cut(s) 28
Ksp22I TGATCA 1 cut(s) 564
Kzo9I GATC 1 cut(s) 564
Lsp1109I GCAGC 1 cut(s) 620
LweI GCATC 1 cut(s) 684
MaeI CTAG 1 cut(s) 42
MaeII ACGT 1 cut(s) 683
MaeIII GTNAC 1 cut(s) 415
MalI GATC 1 cut(s) 566
MboI GATC 1 cut(s) 564
MboII GAAGA 4 cut(s) 151, 535, 721, 746
MfeI CAATTG 1 cut(s) 170
MhlI GDGCHC 2 cut(s) 274, 729
MluCI AATT 6 cut(s) 56, 84, 170, 202, 244, 485
MlyI GAGTC 1 cut(s) 22
MmeI TCCRAC 1 cut(s) 357
MnlI CCTC 5 cut(s) 276, 367, 378, 487, 737
MroNI GCCGGC 1 cut(s) 26
MseI TTAA 2 cut(s) 75, 243
MspI CCGG 4 cut(s) 27, 63, 116, 209
MspR9I CCNGG 3 cut(s) 63, 64, 650
MunI CAATTG 1 cut(s) 170
MvaI CCWGG 1 cut(s) 650
MwoI GCNNNNNNNGC 1 cut(s) 368
NaeI GCCGGC 1 cut(s) 28
NciI CCSGG 2 cut(s) 63, 64
NdeI CATATG 1 cut(s) 442
NdeII GATC 1 cut(s) 564
NgoMIV GCCGGC 1 cut(s) 26
NlaIII CATG 2 cut(s) 514, 565
NmuCI GTSAC 1 cut(s) 415
PdiI GCCGGC 1 cut(s) 28
PkrI GCNGC 2 cut(s) 315, 610
PleI GAGTC 1 cut(s) 21
PpsI GAGTC 1 cut(s) 21
Psp124BI GAGCTC 1 cut(s) 729
Psp1406I AACGTT 1 cut(s) 683
Psp6I CCWGG 1 cut(s) 648
PspFI CCCAGC 1 cut(s) 635
PspGI CCWGG 1 cut(s) 648
PspPI GGNCC 3 cut(s) 24, 151, 536
PstI CTGCAG 1 cut(s) 364
RsaI GTAC 3 cut(s) 455, 548, 560
RsaNI GTAC 3 cut(s) 454, 547, 559
SacI GAGCTC 1 cut(s) 729
SaqAI TTAA 2 cut(s) 75, 243
SatI GCNGC 2 cut(s) 314, 609
Sau3AI GATC 1 cut(s) 564
Sau96I GGNCC 3 cut(s) 24, 151, 536
SchI GAGTC 1 cut(s) 22
ScrFI CCNGG 3 cut(s) 63, 64, 650
SduI GDGCHC 2 cut(s) 274, 729
SetI ASST 6 cut(s) 112, 389, 431, 686, 729, 770
SfaNI GCATC 1 cut(s) 684
SfcI CTRYAG 1 cut(s) 360
SinI GGWCC 2 cut(s) 151, 536
SmaI CCCGGG 1 cut(s) 64
SmlI CTYRAG 2 cut(s) 105, 698
SmoI CTYRAG 2 cut(s) 105, 698
Sse9I AATT 6 cut(s) 56, 84, 170, 202, 244, 485
SsiI CCGC 2 cut(s) 313, 591
SspMI CTAG 1 cut(s) 42
SstI GAGCTC 1 cut(s) 729
StyD4I CCNGG 3 cut(s) 61, 62, 648
StyI CCWWGG 1 cut(s) 616
TaaI ACNGT 5 cut(s) 70, 230, 269, 585, 629
TaiI ACGT 1 cut(s) 686
TaqI TCGA 2 cut(s) 48, 296
TaqII GACCGA 1 cut(s) 553
TasI AATT 6 cut(s) 56, 84, 170, 202, 244, 485
TatI WGTACW 1 cut(s) 558
TauI GCSGC 1 cut(s) 316
Tru1I TTAA 2 cut(s) 75, 243
Tru9I TTAA 2 cut(s) 75, 243
TscAI CASTG 4 cut(s) 75, 256, 352, 681
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 1 cut(s) 608
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 3 cut(s) 152, 460, 499
TspMI CCCGGG 1 cut(s) 62
TspRI CASTG 4 cut(s) 75, 256, 352, 681
VpaK11BI GGWCC 2 cut(s) 151, 536
XapI RAATTY 4 cut(s) 56, 84, 202, 485
XmaI CCCGGG 1 cut(s) 62
XspI CTAG 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.