FvH4_6g29770

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001282
Physical Location & Seq
Reverse (-)
23005905 .. 23006984
1080 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g29770.t1

Sequence Viewer

Length: 663 bp
ATGGAGAGCCAGAGGAAGATGATGGTCGTTTTTGGGTTAGTCGCGGCTGTGTTTTTACACAGCGTGACAGCGCAGACGGCACATGTGGTGGGAGGTAGCATAGGTTGGATGATACCACAGAATGGTGCGCAAGAGTATGTCACTTGGGCTTCTGGTCAGGAGTTCATAGTTGGTGATACCCTAATTTTCAACTTTGCGACGGACACACACGACGTAGTGCAAGTGCCCAAAGCATCTTTCGACTCATGCAGCTCCGCTAACCCAATAGGCAGCAGCATCACCACCGGTCCGGCCAACATCACTCTCACAACCACCGGCGACCACTACTACATATGCACTTACGGCCGACACTGCCAGTCCGGCCAGAAGCTCGCCATCACTGTCTCAGCATCCGCGACGTCACCCGGAGCCTCCCCTTCAGCACCAACTCCCACGTCCCCAGCGCCACCCACTACCACTACTCCTCCTACCACCCCTTCACCCACATCTGACGCCCCTTGCCCTCCGATCCAGGCACCATCTCCGTCCACCACTACCATGGGGCCCACAACTAGCATAACGCCGGGATCCACCGCTGTGCCACCGAGTTCTTCTAATTCCCCTGCGGTTGAGGCTGGTCTCGTTTTGTGCTTCTTGTCCCTTGCAATGGTACTTTTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

221

Amino Acids

22.49

Weight (kDa)

5.28

Isoelectric Point (pI)

51.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 36 - 122 2.1e-25 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 401
Acc16I TGCGCA 1 cut(s) 129
AccB1I GGYRCC 1 cut(s) 514
AccB7I CCANNNNNTGG 1 cut(s) 122
AccII CGCG 2 cut(s) 44, 395
AciI CCGC 5 cut(s) 44, 255, 393, 573, 605
AclWI GGATC 3 cut(s) 502, 561, 574
AcoI YGGCCR 3 cut(s) 291, 343, 361
AcuI CTGAAG 1 cut(s) 402
AcyI GRCGYC 2 cut(s) 398, 492
AdeI CACNNNGTG 1 cut(s) 64
AfaI GTAC 1 cut(s) 651
AfiI CCNNNNNNNGG 2 cut(s) 122, 646
AflIII ACRYGT 1 cut(s) 82
AgeI ACCGGT 1 cut(s) 284
AgsI TTSAA 1 cut(s) 190
AjiI CACGTC 1 cut(s) 435
AjnI CCWGG 1 cut(s) 510
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AleI CACNNNNGTG 1 cut(s) 575
AluBI AGCT 2 cut(s) 252, 370
AluI AGCT 2 cut(s) 252, 370
Alw26I GTCTC 2 cut(s) 388, 623
AlwI GGATC 3 cut(s) 502, 561, 574
AoxI GGCC 4 cut(s) 291, 343, 361, 542
ApaI GGGCCC 1 cut(s) 546
ApeKI GCWGC 3 cut(s) 249, 270, 273
AsiGI ACCGGT 1 cut(s) 284
AspLEI GCGC 3 cut(s) 73, 130, 445
AspS9I GGNCC 3 cut(s) 287, 542, 543
AsuC2I CCSGG 2 cut(s) 405, 564
AsuHPI GGTGA 4 cut(s) 185, 271, 393, 471
AvaII GGWCC 1 cut(s) 287
BaeGI GKGCMC 2 cut(s) 228, 546
BaeI ACNNNNGTAYC 1 cut(s) 641
BamHI GGATCC 1 cut(s) 566
BanI GGYRCC 1 cut(s) 514
BanII GRGCYC 1 cut(s) 546
BbvI GCAGC 3 cut(s) 261, 282, 285
BccI CCATC 3 cut(s) 16, 383, 526
BceAI ACGGC 2 cut(s) 93, 358
BciT130I CCWGG 1 cut(s) 512
BcnI CCSGG 2 cut(s) 405, 564
BcoDI GTCTC 2 cut(s) 388, 623
BfaI CTAG 1 cut(s) 552
BfoI RGCGCY 1 cut(s) 446
BglI GCCNNNNNGGC 1 cut(s) 360
BisI GCNGC 4 cut(s) 45, 250, 271, 274
BlsI GCNGC 4 cut(s) 46, 251, 272, 275
Bme1390I CCNGG 3 cut(s) 405, 512, 564
Bme18I GGWCC 1 cut(s) 287
BmgBI CACGTC 1 cut(s) 435
BmgT120I GGNCC 3 cut(s) 287, 542, 543
BmiI GGNNCC 5 cut(s) 409, 516, 543, 544, 568
BmrFI CCNGG 3 cut(s) 405, 512, 564
BmsI GCATC 3 cut(s) 242, 285, 398
BpuMI CCSGG 2 cut(s) 405, 564
BsaHI GRCGYC 2 cut(s) 398, 492
BsaI GGTCTC 1 cut(s) 623
BsaJI CCNNGG 1 cut(s) 537
BsaWI WCCGGW 1 cut(s) 284
BsaXI ACNNNNNCTCC 4 cut(s) 445, 448, 475, 478
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 646
Bse118I RCCGGY 2 cut(s) 284, 314
Bse1I ACTGG 1 cut(s) 355
Bse3DI GCAATG 1 cut(s) 651
BseBI CCWGG 1 cut(s) 512
BseDI CCNNGG 1 cut(s) 537
BseGI GGATG 2 cut(s) 114, 389
BseLI CCNNNNNNNGG 2 cut(s) 122, 646
BseMI GCAATG 1 cut(s) 651
BseMII CTCAG 1 cut(s) 399
BseNI ACTGG 1 cut(s) 355
BseRI GAGGAG 1 cut(s) 453
BseSI GKGCMC 2 cut(s) 228, 546
BseX3I CGGCCG 1 cut(s) 343
BseXI GCAGC 3 cut(s) 261, 282, 285
BseYI CCCAGC 1 cut(s) 439
Bsh1236I CGCG 2 cut(s) 44, 395
Bsh1285I CGRYCG 1 cut(s) 346
BshFI GGCC 4 cut(s) 293, 345, 363, 544
BshNI GGYRCC 1 cut(s) 514
BshTI ACCGGT 1 cut(s) 284
BsiEI CGRYCG 1 cut(s) 346
BsiSI CCGG 6 cut(s) 285, 290, 315, 360, 405, 563
BslFI GGGAC 2 cut(s) 421, 622
BslI CCNNNNNNNGG 2 cut(s) 122, 646
BsmAI GTCTC 2 cut(s) 388, 623
BsmFI GGGAC 2 cut(s) 421, 622
BsnI GGCC 4 cut(s) 293, 345, 363, 544
Bso31I GGTCTC 1 cut(s) 623
Bsp120I GGGCCC 1 cut(s) 542
Bsp1286I GDGCHC 2 cut(s) 228, 546
Bsp143I GATC 2 cut(s) 507, 566
Bsp19I CCATGG 1 cut(s) 537
BspACI CCGC 5 cut(s) 44, 255, 393, 573, 605
BspANI GGCC 4 cut(s) 293, 345, 363, 544
BspCNI CTCAG 1 cut(s) 398
BspFNI CGCG 2 cut(s) 44, 395
BspLI GGNNCC 5 cut(s) 409, 516, 543, 544, 568
BspPI GGATC 3 cut(s) 502, 561, 574
BspT107I GGYRCC 1 cut(s) 514
BspTNI GGTCTC 1 cut(s) 623
BsrDI GCAATG 1 cut(s) 651
BsrFI RCCGGY 2 cut(s) 284, 314
BsrI ACTGG 1 cut(s) 355
BssAI RCCGGY 2 cut(s) 284, 314
BssECI CCNNGG 1 cut(s) 537
BssMI GATC 2 cut(s) 507, 566
BssNI GRCGYC 2 cut(s) 398, 492
BssT1I CCWWGG 1 cut(s) 537
Bst2UI CCWGG 1 cut(s) 512
Bst4CI ACNGT 1 cut(s) 382
BstACI GRCGYC 2 cut(s) 398, 492
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 1 cut(s) 385
BstDSI CCRYGG 1 cut(s) 537
BstF5I GGATG 2 cut(s) 114, 389
BstFNI CGCG 2 cut(s) 44, 395
BstH2I RGCGCY 1 cut(s) 446
BstHHI GCGC 3 cut(s) 73, 130, 445
BstKTI GATC 2 cut(s) 510, 569
BstMAI GTCTC 2 cut(s) 388, 623
BstMBI GATC 2 cut(s) 507, 566
BstMCI CGRYCG 1 cut(s) 346
BstMWI GCNNNNNNNGC 5 cut(s) 77, 342, 351, 360, 611
BstNI CCWGG 1 cut(s) 512
BstNSI RCATGY 1 cut(s) 86
BstSCI CCNGG 3 cut(s) 403, 510, 562
BstSLI GKGCMC 2 cut(s) 228, 546
BstUI CGCG 2 cut(s) 44, 395
BstV1I GCAGC 3 cut(s) 261, 282, 285
BstX2I RGATCY 1 cut(s) 566
BstXI CCANNNNNNTGG 1 cut(s) 538
BstYI RGATCY 1 cut(s) 566
BstZI CGGCCG 1 cut(s) 343
BsuRI GGCC 4 cut(s) 293, 345, 363, 544
BtgI CCRYGG 1 cut(s) 537
BtrI CACGTC 1 cut(s) 435
BtsCI GGATG 2 cut(s) 114, 389
BtsI GCAGTG 1 cut(s) 349
BtsIMutI CAGTG 2 cut(s) 349, 378
Cac8I GCNNGC 1 cut(s) 372
CfoI GCGC 3 cut(s) 73, 130, 445
Cfr10I RCCGGY 2 cut(s) 284, 314
Cfr13I GGNCC 3 cut(s) 287, 542, 543
CpoI CGGWCCG 1 cut(s) 287
CseI GACGC 1 cut(s) 500
Csp6I GTAC 1 cut(s) 650
CspAI ACCGGT 1 cut(s) 284
CspI CGGWCCG 1 cut(s) 287
CviAII CATG 3 cut(s) 83, 246, 538
CviQI GTAC 1 cut(s) 650
DdeI CTNAG 1 cut(s) 385
DpnI GATC 2 cut(s) 509, 568
DpnII GATC 2 cut(s) 507, 566
DraIII CACNNNGTG 1 cut(s) 64
EaeI YGGCCR 3 cut(s) 291, 343, 361
EagI CGGCCG 1 cut(s) 343
EclXI CGGCCG 1 cut(s) 343
Eco130I CCWWGG 1 cut(s) 537
Eco24I GRGCYC 1 cut(s) 546
Eco31I GGTCTC 1 cut(s) 623
Eco47I GGWCC 1 cut(s) 287
Eco52I CGGCCG 1 cut(s) 343
Eco57I CTGAAG 1 cut(s) 402
EcoO109I RGGNCCY 1 cut(s) 542
EcoRII CCWGG 1 cut(s) 510
EcoT14I CCWWGG 1 cut(s) 537
EcoT38I GRGCYC 1 cut(s) 546
ErhI CCWWGG 1 cut(s) 537
FaeI CATG 3 cut(s) 86, 249, 541
FaiI YATR 9 cut(s) 84, 101, 138, 167, 247, 332, 334, 539, 557
FaqI GGGAC 2 cut(s) 421, 622
FatI CATG 3 cut(s) 82, 245, 537
FauNDI CATATG 1 cut(s) 332
Fnu4HI GCNGC 4 cut(s) 45, 250, 271, 274
FokI GGATG 2 cut(s) 121, 376
FriOI GRGCYC 1 cut(s) 546
Fsp4HI GCNGC 4 cut(s) 45, 250, 271, 274
FspBI CTAG 1 cut(s) 552
FspI TGCGCA 1 cut(s) 129
GlaI GCGC 3 cut(s) 72, 129, 444
GluI GCNGC 4 cut(s) 45, 250, 271, 274
GsaI CCCAGC 1 cut(s) 443
HaeII RGCGCY 1 cut(s) 446
HaeIII GGCC 4 cut(s) 293, 345, 363, 544
HapII CCGG 6 cut(s) 285, 290, 315, 360, 405, 563
HgaI GACGC 1 cut(s) 500
HhaI GCGC 3 cut(s) 73, 130, 445
Hin1I GRCGYC 2 cut(s) 398, 492
Hin1II CATG 3 cut(s) 86, 249, 541
Hin6I GCGC 3 cut(s) 71, 128, 443
HinP1I GCGC 3 cut(s) 71, 128, 443
HinfI GANTC 1 cut(s) 242
HpaII CCGG 6 cut(s) 285, 290, 315, 360, 405, 563
HphI GGTGA 4 cut(s) 185, 271, 393, 471
Hpy166II GTNNAC 1 cut(s) 528
Hpy188I TCNGA 2 cut(s) 490, 507
Hpy188III TCNNGA 1 cut(s) 158
Hpy8I GTNNAC 1 cut(s) 528
Hpy99I CGWCG 3 cut(s) 202, 215, 400
HpyAV CCTTC 2 cut(s) 426, 486
HpyCH4III ACNGT 1 cut(s) 382
HpyCH4IV ACGT 3 cut(s) 213, 398, 434
HpyCH4V TGCA 4 cut(s) 220, 249, 336, 644
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 342, 351, 360, 611
HpyF3I CTNAG 1 cut(s) 385
HpySE526I ACGT 3 cut(s) 213, 398, 434
Hsp92I GRCGYC 2 cut(s) 398, 492
Hsp92II CATG 3 cut(s) 86, 249, 541
HspAI GCGC 3 cut(s) 71, 128, 443
Kzo9I GATC 2 cut(s) 507, 566
LmnI GCTCC 2 cut(s) 257, 407
Lsp1109I GCAGC 3 cut(s) 261, 282, 285
LweI GCATC 3 cut(s) 242, 285, 398
MaeI CTAG 1 cut(s) 552
MaeII ACGT 3 cut(s) 213, 398, 434
MaeIII GTNAC 3 cut(s) 64, 139, 399
MalI GATC 2 cut(s) 509, 568
MboI GATC 2 cut(s) 507, 566
MboII GAAGA 2 cut(s) 28, 582
MflI RGATCY 1 cut(s) 566
MhlI GDGCHC 2 cut(s) 228, 546
MluCI AATT 2 cut(s) 183, 595
MlyI GAGTC 1 cut(s) 236
MmeI TCCRAC 1 cut(s) 86
MnlI CCTC 6 cut(s) 6, 86, 421, 474, 513, 604
MslI CAYNNNNRTG 2 cut(s) 536, 575
MspA1I CMGCKG 1 cut(s) 575
MspI CCGG 6 cut(s) 285, 290, 315, 360, 405, 563
MspR9I CCNGG 3 cut(s) 405, 512, 564
MvaI CCWGG 1 cut(s) 512
MvnI CGCG 2 cut(s) 44, 395
MwoI GCNNNNNNNGC 5 cut(s) 77, 342, 351, 360, 611
NciI CCSGG 2 cut(s) 405, 564
NcoI CCATGG 1 cut(s) 537
NdeI CATATG 1 cut(s) 332
NdeII GATC 2 cut(s) 507, 566
NlaIII CATG 3 cut(s) 86, 249, 541
NlaIV GGNNCC 5 cut(s) 409, 516, 543, 544, 568
NmuCI GTSAC 3 cut(s) 64, 139, 399
NsbI TGCGCA 1 cut(s) 129
NspI RCATGY 1 cut(s) 86
OliI CACNNNNGTG 1 cut(s) 575
PciI ACATGT 1 cut(s) 82
PflMI CCANNNNNTGG 1 cut(s) 122
PinAI ACCGGT 1 cut(s) 284
PkrI GCNGC 4 cut(s) 46, 251, 272, 275
PleI GAGTC 1 cut(s) 236
PpsI GAGTC 1 cut(s) 236
PscI ACATGT 1 cut(s) 82
Psp6I CCWGG 1 cut(s) 510
PspFI CCCAGC 1 cut(s) 439
PspGI CCWGG 1 cut(s) 510
PspN4I GGNNCC 5 cut(s) 409, 516, 543, 544, 568
PspOMI GGGCCC 1 cut(s) 542
PspPI GGNCC 3 cut(s) 287, 542, 543
PsuI RGATCY 1 cut(s) 566
RsaI GTAC 1 cut(s) 651
RsaNI GTAC 1 cut(s) 650
RseI CAYNNNNRTG 2 cut(s) 536, 575
Rsr2I CGGWCCG 1 cut(s) 287
RsrII CGGWCCG 1 cut(s) 287
SatI GCNGC 4 cut(s) 45, 250, 271, 274
Sau3AI GATC 2 cut(s) 507, 566
Sau96I GGNCC 3 cut(s) 287, 542, 543
SchI GAGTC 1 cut(s) 236
ScrFI CCNGG 3 cut(s) 405, 512, 564
SduI GDGCHC 2 cut(s) 228, 546
SetI ASST 7 cut(s) 97, 106, 216, 254, 372, 401, 437
SfaNI GCATC 3 cut(s) 242, 285, 398
SgrAI CRCCGGYG 1 cut(s) 314
SinI GGWCC 1 cut(s) 287
SmiMI CAYNNNNRTG 2 cut(s) 536, 575
Sse9I AATT 2 cut(s) 183, 595
SsiI CCGC 5 cut(s) 44, 255, 393, 573, 605
SspMI CTAG 1 cut(s) 552
StyD4I CCNGG 3 cut(s) 403, 510, 562
StyI CCWWGG 1 cut(s) 537
TaaI ACNGT 1 cut(s) 382
TaiI ACGT 3 cut(s) 216, 401, 437
TaqI TCGA 1 cut(s) 240
TasI AATT 2 cut(s) 183, 595
TauI GCSGC 1 cut(s) 47
TscAI CASTG 2 cut(s) 356, 385
TseFI GTSAC 3 cut(s) 64, 139, 399
TseI GCWGC 3 cut(s) 249, 270, 273
Tsp45I GTSAC 3 cut(s) 64, 139, 399
TspDTI ATGAA 1 cut(s) 154
TspGWI ACGGA 2 cut(s) 215, 513
TspRI CASTG 2 cut(s) 356, 385
Van91I CCANNNNNTGG 1 cut(s) 122
VpaK11BI GGWCC 1 cut(s) 287
XceI RCATGY 1 cut(s) 86
XcmI CCANNNNNNNNNTGG 1 cut(s) 535
XspI CTAG 1 cut(s) 552
ZraI GACGTC 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.