Rroxscaffold_2G00109160

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001282
Physical Location & Seq
Forward (+)
33272213 .. 33272943
731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00109160.1

Sequence Viewer

Length: 519 bp
ATGAAGTTAAAAGGAGGAGCAAGACAATTAGAGGACTTTCAACTCACAAACACGCACGATGTGGTAGAAGTACCCAAAGCATCATTCGACTCATGCAGCTCCTCCAACCCAATCGGCAGCACCATCACCAATGGTCCAGCCAACGTTACTCTCACGTCTGCCGGCGACAACTACTATATCTGCACTTTCGGCCGCCATTGCCGGTACGGACAAAAGCTCGCCATTACCGTCTCAGCATCCTCTACCCGGAGCCTCCCCTTCGCACCAACTCCCGTATCCTCAATGCCACCAACTACCACTACTACTCCTACCACACCTTCACCCACATCTAACGCCCTTGCCCTCGGGTCCAAGTACCATCTCCGTCCACCAAAACCACGGGGTCCACAACTAGCATCACGCCGGGATCCACCGCGTTGTCCCCAAGCGGCGGCCACCGAGGGTGTGCGGGTAGTGCCGCCGCACAAGGCCTCCGAAAATTGGAGGCCTCCGACGAGTTTCGAGTATATATCGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

172

Amino Acids

18.41

Weight (kDa)

9.65

Isoelectric Point (pI)

53.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 16 - 71 3.9e-12 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 415
AciI CCGC 7 cut(s) 193, 413, 428, 431, 448, 458, 461
AclI AACGTT 1 cut(s) 144
AclWI GGATC 2 cut(s) 401, 414
AcoI YGGCCR 2 cut(s) 190, 432
AdeI CACNNNGTG 1 cut(s) 61
AfaI GTAC 3 cut(s) 72, 206, 356
AfiI CCNNNNNNNGG 3 cut(s) 246, 430, 480
AgsI TTSAA 1 cut(s) 41
AjiI CACGTC 1 cut(s) 156
AjuI GAANNNNNNNTTGG 2 cut(s) 68, 100
AluBI AGCT 2 cut(s) 99, 217
AluI AGCT 2 cut(s) 99, 217
Alw26I GTCTC 1 cut(s) 235
AlwI GGATC 2 cut(s) 401, 414
Ama87I CYCGRG 1 cut(s) 344
AoxI GGCC 4 cut(s) 190, 432, 468, 485
ApeKI GCWGC 2 cut(s) 96, 117
AspS9I GGNCC 3 cut(s) 134, 348, 383
AsuC2I CCSGG 2 cut(s) 247, 404
AsuHPI GGTGA 2 cut(s) 118, 312
AvaI CYCGRG 1 cut(s) 344
AvaII GGWCC 3 cut(s) 134, 348, 383
BaeI ACNNNNGTAYC 2 cut(s) 258, 291
BamHI GGATCC 1 cut(s) 406
BbvI GCAGC 2 cut(s) 108, 129
BccI CCATC 2 cut(s) 131, 366
BciVI GTATCC 1 cut(s) 286
BcnI CCSGG 2 cut(s) 247, 404
BcoDI GTCTC 1 cut(s) 235
BfaI CTAG 1 cut(s) 392
BfuI GTATCC 1 cut(s) 286
BisI GCNGC 7 cut(s) 97, 118, 193, 429, 432, 458, 461
BlsI GCNGC 7 cut(s) 98, 119, 194, 430, 433, 459, 462
Bme1390I CCNGG 2 cut(s) 247, 404
Bme18I GGWCC 3 cut(s) 134, 348, 383
BmeT110I CYCGRG 1 cut(s) 344
BmgBI CACGTC 1 cut(s) 156
BmgT120I GGNCC 3 cut(s) 134, 348, 383
BmiI GGNNCC 4 cut(s) 251, 349, 384, 408
BmrFI CCNGG 2 cut(s) 247, 404
BmsI GCATC 3 cut(s) 89, 245, 404
BpuMI CCSGG 2 cut(s) 247, 404
BsaJI CCNNGG 3 cut(s) 343, 377, 438
BsaXI ACNNNNNCTCC 4 cut(s) 289, 319, 455, 485
Bsc4I CCNNNNNNNGG 3 cut(s) 246, 430, 480
Bse118I RCCGGY 2 cut(s) 161, 201
Bse3DI GCAATG 1 cut(s) 196
BseDI CCNNGG 3 cut(s) 343, 377, 438
BseGI GGATG 1 cut(s) 236
BseLI CCNNNNNNNGG 3 cut(s) 246, 430, 480
BseMI GCAATG 1 cut(s) 196
BseMII CTCAG 1 cut(s) 246
BseRI GAGGAG 2 cut(s) 30, 91
BseX3I CGGCCG 1 cut(s) 190
BseXI GCAGC 2 cut(s) 108, 129
BsgI GTGCAG 1 cut(s) 166
Bsh1236I CGCG 1 cut(s) 415
Bsh1285I CGRYCG 1 cut(s) 193
BshFI GGCC 4 cut(s) 192, 434, 470, 487
BsiEI CGRYCG 1 cut(s) 193
BsiHKCI CYCGRG 1 cut(s) 344
BsiSI CCGG 4 cut(s) 162, 202, 247, 403
BslFI GGGAC 1 cut(s) 405
BslI CCNNNNNNNGG 3 cut(s) 246, 430, 480
BsmAI GTCTC 1 cut(s) 235
BsmBI CGTCTC 1 cut(s) 235
BsmFI GGGAC 1 cut(s) 405
BsnI GGCC 4 cut(s) 192, 434, 470, 487
BsoBI CYCGRG 1 cut(s) 344
Bsp143I GATC 1 cut(s) 406
BspACI CCGC 7 cut(s) 193, 413, 428, 431, 448, 458, 461
BspANI GGCC 4 cut(s) 192, 434, 470, 487
BspCNI CTCAG 1 cut(s) 245
BspFNI CGCG 1 cut(s) 415
BspLI GGNNCC 4 cut(s) 251, 349, 384, 408
BspPI GGATC 2 cut(s) 401, 414
BsrDI GCAATG 1 cut(s) 196
BsrFI RCCGGY 2 cut(s) 161, 201
BssAI RCCGGY 2 cut(s) 161, 201
BssECI CCNNGG 3 cut(s) 343, 377, 438
BssMI GATC 1 cut(s) 406
Bst4CI ACNGT 1 cut(s) 229
BstC8I GCNNGC 2 cut(s) 163, 219
BstDEI CTNAG 1 cut(s) 232
BstDSI CCRYGG 1 cut(s) 377
BstF5I GGATG 1 cut(s) 236
BstFNI CGCG 1 cut(s) 415
BstKTI GATC 1 cut(s) 409
BstMAI GTCTC 1 cut(s) 235
BstMBI GATC 1 cut(s) 406
BstMCI CGRYCG 1 cut(s) 193
BstMWI GCNNNNNNNGC 3 cut(s) 189, 198, 454
BstSCI CCNGG 2 cut(s) 245, 402
BstUI CGCG 1 cut(s) 415
BstV1I GCAGC 2 cut(s) 108, 129
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
BstZI CGGCCG 1 cut(s) 190
BsuI GTATCC 1 cut(s) 286
BsuRI GGCC 4 cut(s) 192, 434, 470, 487
BtgI CCRYGG 1 cut(s) 377
BtrI CACGTC 1 cut(s) 156
BtsCI GGATG 1 cut(s) 236
Cac8I GCNNGC 2 cut(s) 163, 219
Cfr10I RCCGGY 2 cut(s) 161, 201
Cfr13I GGNCC 3 cut(s) 134, 348, 383
Csp6I GTAC 3 cut(s) 71, 205, 355
CviAII CATG 1 cut(s) 93
CviJI RGCY 8 cut(s) 99, 140, 192, 217, 252, 434, 470, 487
CviKI_1 RGCY 8 cut(s) 99, 140, 192, 217, 252, 434, 470, 487
CviQI GTAC 3 cut(s) 71, 205, 355
DdeI CTNAG 1 cut(s) 232
DpnI GATC 1 cut(s) 408
DpnII GATC 1 cut(s) 406
DraIII CACNNNGTG 1 cut(s) 61
EaeI YGGCCR 2 cut(s) 190, 432
EagI CGGCCG 1 cut(s) 190
EclXI CGGCCG 1 cut(s) 190
Eco147I AGGCCT 2 cut(s) 470, 487
Eco47I GGWCC 3 cut(s) 134, 348, 383
Eco52I CGGCCG 1 cut(s) 190
Eco88I CYCGRG 1 cut(s) 344
Esp3I CGTCTC 1 cut(s) 235
FaeI CATG 1 cut(s) 96
FaiI YATR 5 cut(s) 94, 177, 507, 509, 517
FaqI GGGAC 1 cut(s) 405
FatI CATG 1 cut(s) 92
FauI CCCGC 1 cut(s) 441
Fnu4HI GCNGC 7 cut(s) 97, 118, 193, 429, 432, 458, 461
FokI GGATG 1 cut(s) 223
Fsp4HI GCNGC 7 cut(s) 97, 118, 193, 429, 432, 458, 461
FspBI CTAG 1 cut(s) 392
GluI GCNGC 7 cut(s) 97, 118, 193, 429, 432, 458, 461
HaeIII GGCC 4 cut(s) 192, 434, 470, 487
HapII CCGG 4 cut(s) 162, 202, 247, 403
Hin1II CATG 1 cut(s) 96
HinfI GANTC 1 cut(s) 89
HpaII CCGG 4 cut(s) 162, 202, 247, 403
HphI GGTGA 2 cut(s) 118, 312
Hpy166II GTNNAC 2 cut(s) 368, 386
Hpy188I TCNGA 2 cut(s) 475, 492
Hpy8I GTNNAC 2 cut(s) 368, 386
Hpy99I CGWCG 1 cut(s) 496
HpyAV CCTTC 2 cut(s) 268, 327
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4IV ACGT 2 cut(s) 144, 155
HpyCH4V TGCA 2 cut(s) 96, 183
HpyF10VI GCNNNNNNNGC 3 cut(s) 189, 198, 454
HpyF3I CTNAG 1 cut(s) 232
HpySE526I ACGT 2 cut(s) 144, 155
Hsp92II CATG 1 cut(s) 96
KroI GCCGGC 1 cut(s) 161
KroNI GCCGGC 1 cut(s) 163
Kzo9I GATC 1 cut(s) 406
LmnI GCTCC 3 cut(s) 17, 104, 249
LpnPI CCDG 5 cut(s) 150, 175, 215, 260, 416
Lsp1109I GCAGC 2 cut(s) 108, 129
LweI GCATC 3 cut(s) 89, 245, 404
MaeI CTAG 1 cut(s) 392
MaeII ACGT 2 cut(s) 144, 155
MaeIII GTNAC 1 cut(s) 145
MalI GATC 1 cut(s) 408
MboI GATC 1 cut(s) 406
MflI RGATCY 1 cut(s) 406
MluCI AATT 2 cut(s) 26, 478
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 2 cut(s) 129, 515
MroNI GCCGGC 1 cut(s) 161
MseI TTAA 1 cut(s) 8
MspI CCGG 4 cut(s) 162, 202, 247, 403
MspR9I CCNGG 2 cut(s) 247, 404
MvnI CGCG 1 cut(s) 415
MwoI GCNNNNNNNGC 3 cut(s) 189, 198, 454
NaeI GCCGGC 1 cut(s) 163
NciI CCSGG 2 cut(s) 247, 404
NdeII GATC 1 cut(s) 406
NgoMIV GCCGGC 1 cut(s) 161
NlaIII CATG 1 cut(s) 96
NlaIV GGNNCC 4 cut(s) 251, 349, 384, 408
PceI AGGCCT 2 cut(s) 470, 487
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PdiI GCCGGC 1 cut(s) 163
PkrI GCNGC 7 cut(s) 98, 119, 194, 430, 433, 459, 462
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
Psp1406I AACGTT 1 cut(s) 144
PspN4I GGNNCC 4 cut(s) 251, 349, 384, 408
PspPI GGNCC 3 cut(s) 134, 348, 383
PsuI RGATCY 1 cut(s) 406
RsaI GTAC 3 cut(s) 72, 206, 356
RsaNI GTAC 3 cut(s) 71, 205, 355
SaqAI TTAA 1 cut(s) 8
SatI GCNGC 7 cut(s) 97, 118, 193, 429, 432, 458, 461
Sau3AI GATC 1 cut(s) 406
Sau96I GGNCC 3 cut(s) 134, 348, 383
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 2 cut(s) 247, 404
SetI ASST 5 cut(s) 101, 147, 158, 219, 319
SfaNI GCATC 3 cut(s) 89, 245, 404
SinI GGWCC 3 cut(s) 134, 348, 383
Sse9I AATT 2 cut(s) 26, 478
SseBI AGGCCT 2 cut(s) 470, 487
SsiI CCGC 7 cut(s) 193, 413, 428, 431, 448, 458, 461
SspMI CTAG 1 cut(s) 392
StuI AGGCCT 2 cut(s) 470, 487
StyD4I CCNGG 2 cut(s) 245, 402
TaaI ACNGT 1 cut(s) 229
TaiI ACGT 2 cut(s) 147, 158
TaqI TCGA 2 cut(s) 87, 501
TasI AATT 2 cut(s) 26, 478
TauI GCSGC 5 cut(s) 195, 431, 434, 460, 463
Tru1I TTAA 1 cut(s) 8
Tru9I TTAA 1 cut(s) 8
TseI GCWGC 2 cut(s) 96, 117
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 222, 353
VpaK11BI GGWCC 3 cut(s) 134, 348, 383
XspI CTAG 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.