Rorug02G0325400

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001282
Physical Location & Seq
Reverse (-)
39905971 .. 39911602
5632 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0325400.1

Sequence Viewer

Length: 1554 bp
ATGTTCAAGTCGAAGCTGCAGGAGCTGTGCCAGAAGAAGGCGTGGCCGTTACCGGAGTACAGCAGCAAGAAGGAGGGCCTCGATCACGACCCTCGTTTCTCAGCCACCGTCAAAATCAACGGCCTTGAATTCAACACTGTCGACCCCTTCAGATCCTCCAAGTTGGCCCAAAACGACGTCGCTAGGCTCGCCTTCGATCACTTCTCTGCCCCCGCTACCCGCCCCATTCCTTTCGATTTCCCTCAGCCGAGTCTTGTCGCTGTCACTCCCTCAGGACTGGGCACTGATCGGAGTTTTACAAACACACCACTGCAATTGCAAAAAACTCAAGAAACACATCAAGCTCCTCAGGTCAGTGTTAATGCAACAGATGGTGGTTCTCAAATCAGTGGTAATGCAACAGTTGATGGTTCTCAAGTCAGTGGTAATGCAACAGTTGCTGGTTTTAAGGTCAGTGGTAATGCAACAGTTTCCGGTTCTCAGGTCAGTGCAACAGGTTCCAGTTCTCAGATCAGTGGCAGAGCATTAGGTGTTGAAGGTGATAAGAGATCTAAAGATATACAACATTTGCATAAGAACCAGCTGCAGTGTTATGCTCAAAAGAGGAATCTCATTTTACCTGTCTACTCTTGTGAGCGGGAAGGTCCTCCTCATGCCGCTCGTTTCAAATGTACAGTAACAATTGATGGAAAAACTTATCAGGGTCAAGAATTTCTTCCCACTGTGAAGGATGCTGAACATGCAGCTGCGGAAGTTGCTTTGATCTCCCTGTTGCCAAATGGAGTCAAAGAAGATGATTCCGGTTTGTACAAGAACCTTTTACAAGAACTAATTCAAAAAGAAGGTCAACATGTACCACTCTATAGCACAAGGACATCTGGTGAACCTCACATGCTTACTTTTGTTTCAACTGTGGAGATTGGAGGAGAATCTTTTACCGGTCAAGTAGCAAGAAGCAAGAAGCTGGCAGAAGTGAGTGCAGCAAAGGTTGCCTATAATACTTTGAGAGATCGTAAGTCAAGTCAGATCCCATTGTTTCTGACCCAGGCTTCACAAGGGCAAGAAGGATTACAGTTCTTATCTTCGAATGCTCCATCATACATGGCTCCTGATCTACAACAACAAGTGAGACCTAACGCTCCCAAGATTTCAATTCCAAATTCAGTTACCCCACAAGAAAAGGAGATCAGCGTGGCTGCTGTGGGAACCAGTCATCAGTCTACAACTGTATCTTCAGGAGTTGATAATCGTACAGTAGGTGGAGGATTCACTTCATCTGGCACTGTACCTGGCCGTGTACTGAAGACTTCACGTTCTGCTTCCCCTGCATCCCAAAGTGGATCCTCTTCGACTTCGACTTATTTCTGTACTTCAAATATGGACTCTACTCTTCAGCCACCAGCGAAAAGAACTACATCATACTCTAACATGGTTGCTGTACATCCACGGATGTTGAATATGACACTCCCAGAAGACTGCGTTGTGTTGTCCCTTAGTGATGAAACTTGGGTTGCTGTATCGCCCATGAAGACAGAAGGTATAACAAATCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

517

Amino Acids

55.37

Weight (kDa)

8.57

Isoelectric Point (pI)

44.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
dsrm PF00035 3 - 65 6.4e-10 Double-stranded RNA binding motif
dsrm PF00035 192 - 256 1.3e-13 Double-stranded RNA binding motif
dsrm PF00035 270 - 335 1.2e-13 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 180
AccBSI CCGCTC 2 cut(s) 637, 659
AccI GTMKAC 3 cut(s) 141, 624, 1220
AciI CCGC 5 cut(s) 213, 220, 637, 657, 749
AclWI GGATC 4 cut(s) 147, 1021, 1335, 1348
AcoI YGGCCR 2 cut(s) 44, 1291
AcsI RAATTY 3 cut(s) 128, 710, 1159
AcuI CTGAAG 4 cut(s) 133, 1218, 1322, 1376
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 9 cut(s) 59, 673, 809, 855, 1252, 1287, 1299, 1369, 1440
AfiI CCNNNNNNNGG 1 cut(s) 37
AflIII ACRYGT 1 cut(s) 850
AgeI ACCGGT 1 cut(s) 938
AjnI CCWGG 2 cut(s) 1044, 1288
AluBI AGCT 6 cut(s) 16, 25, 344, 583, 746, 964
AluI AGCT 6 cut(s) 16, 25, 344, 583, 746, 964
Alw26I GTCTC 1 cut(s) 1123
AlwI GGATC 4 cut(s) 147, 1021, 1335, 1348
AlwNI CAGNNNCTG 2 cut(s) 25, 440
AoxI GGCC 5 cut(s) 44, 76, 121, 165, 1291
ApeKI GCWGC 7 cut(s) 16, 63, 583, 743, 746, 980, 1196
ApoI RAATTY 3 cut(s) 128, 710, 1159
ArsI GACNNNNNNTTYG 2 cut(s) 829, 861
AsiGI ACCGGT 1 cut(s) 938
Asp700I GAANNNNTTC 2 cut(s) 714, 831
AspS9I GGNCC 3 cut(s) 76, 166, 644
AsuHPI GGTGA 2 cut(s) 551, 893
AsuII TTCGAA 1 cut(s) 1085
AvaII GGWCC 1 cut(s) 644
AxyI CCTNAGG 2 cut(s) 271, 348
BaeGI GKGCMC 1 cut(s) 284
BaeI ACNNNNGTAYC 2 cut(s) 1212, 1245
BamHI GGATCC 1 cut(s) 1340
BbsI GAAGAC 3 cut(s) 1310, 1479, 1535
BbvCI CCTCAGC 1 cut(s) 243
BbvI GCAGC 7 cut(s) 3, 75, 570, 733, 755, 992, 1183
BccI CCATC 4 cut(s) 365, 401, 680, 1102
BceAI ACGGC 3 cut(s) 31, 136, 1278
BciT130I CCWGG 2 cut(s) 1046, 1290
BcoDI GTCTC 1 cut(s) 1123
BfaI CTAG 1 cut(s) 183
BfmI CTRYAG 3 cut(s) 17, 584, 862
BglII AGATCT 1 cut(s) 548
BisI GCNGC 8 cut(s) 17, 64, 584, 657, 744, 747, 981, 1197
BlsI GCNGC 8 cut(s) 18, 65, 585, 658, 745, 748, 982, 1198
Bme1390I CCNGG 2 cut(s) 1046, 1290
Bme18I GGWCC 1 cut(s) 644
BmgT120I GGNCC 3 cut(s) 76, 166, 644
BmiI GGNNCC 4 cut(s) 499, 1107, 1207, 1342
BmrFI CCNGG 2 cut(s) 1046, 1290
BmrI ACTGGG 1 cut(s) 287
BmsI GCATC 2 cut(s) 721, 1337
BmuI ACTGGG 1 cut(s) 287
BpiI GAAGAC 3 cut(s) 1310, 1479, 1535
Bpu10I CCTNAGC 1 cut(s) 243
Bpu14I TTCGAA 1 cut(s) 1085
BpuEI CTTGAG 2 cut(s) 312, 399
BsaHI GRCGYC 1 cut(s) 177
BsaI GGTCTC 1 cut(s) 1123
BsaJI CCNNGG 2 cut(s) 1044, 1445
BsaWI WCCGGW 4 cut(s) 52, 473, 800, 938
Bsc4I CCNNNNNNNGG 1 cut(s) 37
Bse118I RCCGGY 1 cut(s) 938
Bse1I ACTGG 3 cut(s) 282, 501, 1209
Bse21I CCTNAGG 2 cut(s) 271, 348
BseBI CCWGG 2 cut(s) 1046, 1290
BseDI CCNNGG 2 cut(s) 1044, 1445
BseGI GGATG 4 cut(s) 736, 1328, 1441, 1455
BseLI CCNNNNNNNGG 1 cut(s) 37
BseMII CTCAG 6 cut(s) 114, 257, 285, 362, 494, 521
BseNI ACTGG 3 cut(s) 282, 501, 1209
BseRI GAGGAG 3 cut(s) 336, 639, 939
BseSI GKGCMC 1 cut(s) 284
BseXI GCAGC 7 cut(s) 3, 75, 570, 733, 755, 992, 1183
BsgI GTGCAG 1 cut(s) 999
BshFI GGCC 5 cut(s) 46, 78, 123, 167, 1293
BshTI ACCGGT 1 cut(s) 938
BsiSI CCGG 4 cut(s) 53, 474, 801, 939
BslFI GGGAC 1 cut(s) 1474
BslI CCNNNNNNNGG 1 cut(s) 37
BsmAI GTCTC 1 cut(s) 1123
BsmFI GGGAC 1 cut(s) 1474
BsmI GAATGC 1 cut(s) 1093
BsnI GGCC 5 cut(s) 46, 78, 123, 167, 1293
Bso31I GGTCTC 1 cut(s) 1123
Bsp119I TTCGAA 1 cut(s) 1085
Bsp1286I GDGCHC 1 cut(s) 284
Bsp1407I TGTACA 3 cut(s) 671, 807, 1438
BspACI CCGC 5 cut(s) 213, 220, 637, 657, 749
BspANI GGCC 5 cut(s) 46, 78, 123, 167, 1293
BspCNI CTCAG 6 cut(s) 113, 256, 284, 361, 493, 520
BspLI GGNNCC 4 cut(s) 499, 1107, 1207, 1342
BspMAI CTGCAG 2 cut(s) 21, 588
BspPI GGATC 4 cut(s) 147, 1021, 1335, 1348
BspT104I TTCGAA 1 cut(s) 1085
BspTNI GGTCTC 1 cut(s) 1123
BsrBI CCGCTC 2 cut(s) 637, 659
BsrFI RCCGGY 1 cut(s) 938
BsrGI TGTACA 3 cut(s) 671, 807, 1438
BsrI ACTGG 3 cut(s) 282, 501, 1209
BssAI RCCGGY 1 cut(s) 938
BssECI CCNNGG 2 cut(s) 1044, 1445
BssNI GRCGYC 1 cut(s) 177
Bst2UI CCWGG 2 cut(s) 1046, 1290
Bst6I CTCTTC 2 cut(s) 1351, 1395
BstACI GRCGYC 1 cut(s) 177
BstAPI GCANNNNNTGC 2 cut(s) 437, 989
BstAUI TGTACA 3 cut(s) 671, 807, 1438
BstBI TTCGAA 1 cut(s) 1085
BstC8I GCNNGC 2 cut(s) 189, 966
BstDEI CTNAG 7 cut(s) 100, 243, 271, 348, 480, 507, 1493
BstDSI CCRYGG 1 cut(s) 1445
BstF5I GGATG 4 cut(s) 736, 1328, 1441, 1455
BstMAI GTCTC 1 cut(s) 1123
BstMWI GCNNNNNNNGC 7 cut(s) 22, 188, 437, 740, 755, 989, 1325
BstNI CCWGG 2 cut(s) 1046, 1290
BstNSI RCATGY 3 cut(s) 743, 854, 895
BstSCI CCNGG 2 cut(s) 1044, 1288
BstSFI CTRYAG 3 cut(s) 17, 584, 862
BstSLI GKGCMC 1 cut(s) 284
BstV1I GCAGC 7 cut(s) 3, 75, 570, 733, 755, 992, 1183
BstV2I GAAGAC 3 cut(s) 1310, 1479, 1535
BstX2I RGATCY 4 cut(s) 152, 548, 1026, 1340
BstYI RGATCY 4 cut(s) 152, 548, 1026, 1340
Bsu36I CCTNAGG 2 cut(s) 271, 348
BsuRI GGCC 5 cut(s) 46, 78, 123, 167, 1293
BtgI CCRYGG 1 cut(s) 1445
BtsCI GGATG 4 cut(s) 736, 1328, 1441, 1455
BtsI GCAGTG 2 cut(s) 308, 593
Cac8I GCNNGC 2 cut(s) 189, 966
CaiI CAGNNNCTG 2 cut(s) 25, 440
Cfr10I RCCGGY 1 cut(s) 938
Cfr13I GGNCC 3 cut(s) 76, 166, 644
Csp6I GTAC 9 cut(s) 58, 672, 808, 854, 1251, 1286, 1298, 1368, 1439
CspAI ACCGGT 1 cut(s) 938
CspCI CAANNNNNGTGG 4 cut(s) 297, 332, 1434, 1469
CviAII CATG 7 cut(s) 653, 740, 851, 892, 1102, 1429, 1525
CviQI GTAC 9 cut(s) 58, 672, 808, 854, 1251, 1286, 1298, 1368, 1439
DdeI CTNAG 7 cut(s) 100, 243, 271, 348, 480, 507, 1493
EaeI YGGCCR 2 cut(s) 44, 1291
Eam1104I CTCTTC 2 cut(s) 1351, 1395
EarI CTCTTC 2 cut(s) 1351, 1395
Eco31I GGTCTC 1 cut(s) 1123
Eco47I GGWCC 1 cut(s) 644
Eco57I CTGAAG 4 cut(s) 133, 1218, 1322, 1376
Eco81I CCTNAGG 2 cut(s) 271, 348
EcoO109I RGGNCCY 2 cut(s) 76, 644
EcoRI GAATTC 1 cut(s) 128
EcoRII CCWGG 2 cut(s) 1044, 1288
FaeI CATG 7 cut(s) 656, 743, 854, 895, 1105, 1432, 1528
FalI AAGNNNNNCTT 2 cut(s) 699, 731
FaqI GGGAC 1 cut(s) 1474
FatI CATG 7 cut(s) 652, 739, 850, 891, 1101, 1428, 1524
FauI CCCGC 3 cut(s) 220, 227, 630
FblI GTMKAC 3 cut(s) 141, 624, 1220
Fnu4HI GCNGC 8 cut(s) 17, 64, 584, 657, 744, 747, 981, 1197
FokI GGATG 4 cut(s) 743, 1315, 1428, 1462
Fsp4HI GCNGC 8 cut(s) 17, 64, 584, 657, 744, 747, 981, 1197
FspBI CTAG 1 cut(s) 183
GluI GCNGC 8 cut(s) 17, 64, 584, 657, 744, 747, 981, 1197
HaeIII GGCC 5 cut(s) 46, 78, 123, 167, 1293
HapII CCGG 4 cut(s) 53, 474, 801, 939
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 7 cut(s) 656, 743, 854, 895, 1105, 1432, 1528
HincII GTYRAC 2 cut(s) 142, 848
HindII GTYRAC 2 cut(s) 142, 848
HinfI GANTC 7 cut(s) 250, 607, 783, 797, 929, 1266, 1382
HpaII CCGG 4 cut(s) 53, 474, 801, 939
HphI GGTGA 2 cut(s) 551, 893
Hpy166II GTNNAC 6 cut(s) 142, 625, 848, 884, 1221, 1298
Hpy188I TCNGA 5 cut(s) 152, 291, 510, 1026, 1041
Hpy188III TCNNGA 6 cut(s) 86, 273, 329, 707, 1109, 1236
Hpy8I GTNNAC 6 cut(s) 142, 625, 848, 884, 1221, 1298
Hpy99I CGWCG 2 cut(s) 179, 182
HpyCH4IV ACGT 2 cut(s) 177, 1312
HpyF10VI GCNNNNNNNGC 7 cut(s) 22, 188, 437, 740, 755, 989, 1325
HpyF3I CTNAG 7 cut(s) 100, 243, 271, 348, 480, 507, 1493
HpySE526I ACGT 2 cut(s) 177, 1312
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 7 cut(s) 656, 743, 854, 895, 1105, 1432, 1528
LmnI GCTCC 5 cut(s) 22, 349, 1096, 1111, 1144
Lsp1109I GCAGC 7 cut(s) 3, 75, 570, 733, 755, 992, 1183
LweI GCATC 2 cut(s) 721, 1337
MaeI CTAG 1 cut(s) 183
MaeII ACGT 2 cut(s) 177, 1312
MaeIII GTNAC 4 cut(s) 48, 262, 676, 1165
MbiI CCGCTC 2 cut(s) 637, 659
MfeI CAATTG 2 cut(s) 314, 681
MflI RGATCY 4 cut(s) 152, 548, 1026, 1340
MhlI GDGCHC 1 cut(s) 284
MluCI AATT 7 cut(s) 128, 314, 681, 710, 831, 1152, 1159
MlyI GAGTC 3 cut(s) 259, 792, 1376
MroXI GAANNNNTTC 2 cut(s) 714, 831
MseI TTAA 2 cut(s) 360, 447
MspA1I CMGCKG 2 cut(s) 583, 746
MspI CCGG 4 cut(s) 53, 474, 801, 939
MspR9I CCNGG 2 cut(s) 1046, 1290
MunI CAATTG 2 cut(s) 314, 681
Mva1269I GAATGC 1 cut(s) 1093
MvaI CCWGG 2 cut(s) 1046, 1290
MwoI GCNNNNNNNGC 7 cut(s) 22, 188, 437, 740, 755, 989, 1325
NlaIII CATG 7 cut(s) 656, 743, 854, 895, 1105, 1432, 1528
NlaIV GGNNCC 4 cut(s) 499, 1107, 1207, 1342
NmeAIII GCCGAG 1 cut(s) 273
NmuCI GTSAC 1 cut(s) 262
NspI RCATGY 3 cut(s) 743, 854, 895
NspV TTCGAA 1 cut(s) 1085
PciI ACATGT 1 cut(s) 850
PctI GAATGC 1 cut(s) 1093
PdmI GAANNNNTTC 2 cut(s) 714, 831
PfeI GAWTC 4 cut(s) 607, 797, 929, 1266
PinAI ACCGGT 1 cut(s) 938
PkrI GCNGC 8 cut(s) 18, 65, 585, 658, 745, 748, 982, 1198
PleI GAGTC 3 cut(s) 258, 791, 1376
PpsI GAGTC 3 cut(s) 258, 791, 1376
PpuMI RGGWCCY 1 cut(s) 644
PscI ACATGT 1 cut(s) 850
Psp5II RGGWCCY 1 cut(s) 644
Psp6I CCWGG 2 cut(s) 1044, 1288
PspGI CCWGG 2 cut(s) 1044, 1288
PspN4I GGNNCC 4 cut(s) 499, 1107, 1207, 1342
PspPI GGNCC 3 cut(s) 76, 166, 644
PspPPI RGGWCCY 1 cut(s) 644
PstI CTGCAG 2 cut(s) 21, 588
PstNI CAGNNNCTG 2 cut(s) 25, 440
PsuI RGATCY 4 cut(s) 152, 548, 1026, 1340
PvuII CAGCTG 2 cut(s) 583, 746
RsaI GTAC 9 cut(s) 59, 673, 809, 855, 1252, 1287, 1299, 1369, 1440
RsaNI GTAC 9 cut(s) 58, 672, 808, 854, 1251, 1286, 1298, 1368, 1439
SalI GTCGAC 1 cut(s) 140
SaqAI TTAA 2 cut(s) 360, 447
SatI GCNGC 8 cut(s) 17, 64, 584, 657, 744, 747, 981, 1197
Sau96I GGNCC 3 cut(s) 76, 166, 644
SchI GAGTC 3 cut(s) 259, 792, 1376
ScrFI CCNGG 2 cut(s) 1046, 1290
SduI GDGCHC 1 cut(s) 284
SfaNI GCATC 2 cut(s) 721, 1337
SfcI CTRYAG 3 cut(s) 17, 584, 862
SfuI TTCGAA 1 cut(s) 1085
SinI GGWCC 1 cut(s) 644
SmlI CTYRAG 2 cut(s) 327, 414
SmoI CTYRAG 2 cut(s) 327, 414
Sse9I AATT 7 cut(s) 128, 314, 681, 710, 831, 1152, 1159
SsiI CCGC 5 cut(s) 213, 220, 637, 657, 749
SspMI CTAG 1 cut(s) 183
StyD4I CCNGG 2 cut(s) 1044, 1288
TaiI ACGT 2 cut(s) 180, 1315
TaqI TCGA 8 cut(s) 11, 81, 141, 195, 234, 1085, 1349, 1355
TasI AATT 7 cut(s) 128, 314, 681, 710, 831, 1152, 1159
TatI WGTACW 6 cut(s) 57, 671, 807, 1297, 1367, 1438
TauI GCSGC 1 cut(s) 659
TfiI GAWTC 4 cut(s) 607, 797, 929, 1266
Tru1I TTAA 2 cut(s) 360, 447
Tru9I TTAA 2 cut(s) 360, 447
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 7 cut(s) 16, 63, 583, 743, 746, 980, 1196
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 3 cut(s) 1263, 1515, 1541
TspGWI ACGGA 1 cut(s) 1462
VpaK11BI GGWCC 1 cut(s) 644
XapI RAATTY 3 cut(s) 128, 710, 1159
XceI RCATGY 3 cut(s) 743, 854, 895
XmiI GTMKAC 3 cut(s) 141, 624, 1220
XmnI GAANNNNTTC 2 cut(s) 714, 831
XspI CTAG 1 cut(s) 183
ZraI GACGTC 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.