Rorug02G0325600

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001282
Physical Location & Seq
Reverse (-)
39943867 .. 39944700
834 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0325600.1

Sequence Viewer

Length: 726 bp
ATGGGGACACTTGAGGCTTTTGATCAGAGGTTAATGAGTCATGATGAACTATCTGAAAAAGCTTTTCAAACACTGAGTCTTAGCTCAAATAAGAAGAATGATGAGCCATCTAGTTCTAATTCAAAAAAGGGAAACCAAAAGATGAAGAAGAATTGGAAAAACAGAGGCAAGAAGTGGGAAGAAAAACCAAAGTGTACAAATTGTCACAGGTTTGGTCATGTCAAGAAGGATTGTAACTTTAAAGGCAATCAACAAGCAAACTGCATTGAAGAACAGTGTGATACCAACATATTCTCTGTAAGCCATGATGTAATTACACAAGTGAGTGAGAATGTGTGGTTTGTTGATAGTGCTTGCAGTAATCATATGATTGCGAATGCTAATCTTCTGTATGATATTGACTACAATAGCATCATTAAAGTGAAGATGGGAAATGGAATGTTGGTTGACACAAAAGGCAAAGGAAGCATTGCGGTGGAAACCAAAAATGGAAAAATGTTCATCAAGGATGTAATATTAGTTCCAGATCTTGATTCAAATTTGCTAAGTATTGGTCAGCTCATTGAGCACTCATATCACCTACACTTTGGTGACAATACTTGCAAGATTTTCGAGAGAGGGAGCAGCAAGAAACTGATGGTAGAAGGTTTTCCTTTGACTCACAAATATGCAATAGAGAGTGCAAGAAAAATGGAGCTACAGGATGATCCTTGGCTCTGGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

241

Amino Acids

27.49

Weight (kDa)

8.09

Isoelectric Point (pI)

38.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 113 - 192 5e-22 Pol polyprotein, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 473
AclWI GGATC 1 cut(s) 701
AcsI RAATTY 1 cut(s) 538
AfaI GTAC 1 cut(s) 196
AgsI TTSAA 4 cut(s) 68, 123, 269, 537
AleI CACNNNNGTG 1 cut(s) 588
AluBI AGCT 4 cut(s) 62, 84, 559, 697
AluI AGCT 4 cut(s) 62, 84, 559, 697
Alw21I GWGCWC 1 cut(s) 570
AlwI GGATC 1 cut(s) 701
ApeKI GCWGC 1 cut(s) 624
ApoI RAATTY 1 cut(s) 538
ArsI GACNNNNNNTTYG 2 cut(s) 61, 93
Asp700I GAANNNNTTC 1 cut(s) 648
AsuHPI GGTGA 2 cut(s) 569, 602
Bbv12I GWGCWC 1 cut(s) 570
BbvI GCAGC 1 cut(s) 636
BccI CCATC 3 cut(s) 115, 421, 631
BcgI CGANNNNNNTGC 2 cut(s) 592, 626
BclI TGATCA 1 cut(s) 22
BfaI CTAG 1 cut(s) 111
BfmI CTRYAG 1 cut(s) 698
BglII AGATCT 1 cut(s) 526
BisI GCNGC 1 cut(s) 625
BlsI GCNGC 1 cut(s) 626
BmsI GCATC 1 cut(s) 420
BpuEI CTTGAG 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 710
Bse3DI GCAATG 1 cut(s) 468
BseDI CCNNGG 1 cut(s) 710
BseGI GGATG 2 cut(s) 514, 709
BseMI GCAATG 1 cut(s) 468
BseMII CTCAG 1 cut(s) 65
BseXI GCAGC 1 cut(s) 636
BsiHKAI GWGCWC 1 cut(s) 570
BslFI GGGAC 1 cut(s) 19
BsmFI GGGAC 1 cut(s) 19
BsmI GAATGC 1 cut(s) 382
Bsp1286I GDGCHC 1 cut(s) 570
Bsp1407I TGTACA 1 cut(s) 194
Bsp143I GATC 3 cut(s) 22, 526, 706
BspACI CCGC 1 cut(s) 473
BspCNI CTCAG 1 cut(s) 66
BspHI TCATGA 1 cut(s) 40
BspPI GGATC 1 cut(s) 701
BsrDI GCAATG 1 cut(s) 468
BsrGI TGTACA 1 cut(s) 194
BssECI CCNNGG 1 cut(s) 710
BssMI GATC 3 cut(s) 22, 526, 706
BssT1I CCWWGG 1 cut(s) 710
Bst4CI ACNGT 1 cut(s) 276
BstAUI TGTACA 1 cut(s) 194
BstC8I GCNNGC 1 cut(s) 355
BstDEI CTNAG 3 cut(s) 74, 80, 545
BstF5I GGATG 2 cut(s) 514, 709
BstKTI GATC 3 cut(s) 25, 529, 709
BstMBI GATC 3 cut(s) 22, 526, 706
BstMWI GCNNNNNNNGC 2 cut(s) 465, 565
BstSFI CTRYAG 1 cut(s) 698
BstV1I GCAGC 1 cut(s) 636
BstX2I RGATCY 1 cut(s) 526
BstYI RGATCY 1 cut(s) 526
BtsCI GGATG 2 cut(s) 514, 709
BtsIMutI CAGTG 2 cut(s) 71, 281
Cac8I GCNNGC 1 cut(s) 355
CciI TCATGA 1 cut(s) 40
Csp6I GTAC 1 cut(s) 195
CviAII CATG 3 cut(s) 41, 218, 305
CviJI RGCY 8 cut(s) 17, 62, 84, 106, 303, 559, 697, 715
CviKI_1 RGCY 8 cut(s) 17, 62, 84, 106, 303, 559, 697, 715
CviQI GTAC 1 cut(s) 195
DdeI CTNAG 3 cut(s) 74, 80, 545
DpnI GATC 3 cut(s) 24, 528, 708
DpnII GATC 3 cut(s) 22, 526, 706
DraI TTTAAA 1 cut(s) 241
Eco130I CCWWGG 1 cut(s) 710
EcoT14I CCWWGG 1 cut(s) 710
ErhI CCWWGG 1 cut(s) 710
FaeI CATG 3 cut(s) 44, 221, 308
FaiI YATR 9 cut(s) 42, 219, 290, 306, 366, 368, 393, 574, 669
FaqI GGGAC 1 cut(s) 19
FatI CATG 3 cut(s) 40, 217, 304
FauNDI CATATG 1 cut(s) 366
FbaI TGATCA 1 cut(s) 22
Fnu4HI GCNGC 1 cut(s) 625
FokI GGATG 2 cut(s) 521, 716
Fsp4HI GCNGC 1 cut(s) 625
FspBI CTAG 1 cut(s) 111
GluI GCNGC 1 cut(s) 625
Hin1II CATG 3 cut(s) 44, 221, 308
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HindIII AAGCTT 1 cut(s) 60
HinfI GANTC 4 cut(s) 37, 76, 533, 658
HphI GGTGA 2 cut(s) 569, 602
Hpy166II GTNNAC 2 cut(s) 195, 448
Hpy188I TCNGA 2 cut(s) 27, 55
Hpy188III TCNNGA 5 cut(s) 41, 223, 524, 530, 613
Hpy8I GTNNAC 2 cut(s) 195, 448
HpyAV CCTTC 2 cut(s) 220, 638
HpyCH4III ACNGT 1 cut(s) 276
HpyCH4V TGCA 5 cut(s) 264, 357, 603, 671, 683
HpyF10VI GCNNNNNNNGC 2 cut(s) 465, 565
HpyF3I CTNAG 3 cut(s) 74, 80, 545
Hsp92II CATG 3 cut(s) 44, 221, 308
Ksp22I TGATCA 1 cut(s) 22
Kzo9I GATC 3 cut(s) 22, 526, 706
LmnI GCTCC 2 cut(s) 621, 694
LpnPI CCDG 4 cut(s) 193, 537, 686, 703
Lsp1109I GCAGC 1 cut(s) 636
LweI GCATC 1 cut(s) 420
MaeI CTAG 1 cut(s) 111
MaeIII GTNAC 3 cut(s) 203, 233, 590
MalI GATC 3 cut(s) 24, 528, 708
MboI GATC 3 cut(s) 22, 526, 706
MboII GAAGA 7 cut(s) 106, 157, 160, 191, 281, 377, 436
MflI RGATCY 1 cut(s) 526
MhlI GDGCHC 1 cut(s) 570
MluCI AATT 5 cut(s) 118, 151, 199, 312, 538
MlyI GAGTC 3 cut(s) 46, 85, 652
MnlI CCTC 4 cut(s) 7, 21, 158, 611
MroXI GAANNNNTTC 1 cut(s) 648
MseI TTAA 3 cut(s) 32, 240, 417
MslI CAYNNNNRTG 4 cut(s) 419, 473, 588, 666
Mva1269I GAATGC 1 cut(s) 382
MwoI GCNNNNNNNGC 2 cut(s) 465, 565
NdeI CATATG 1 cut(s) 366
NdeII GATC 3 cut(s) 22, 526, 706
NlaIII CATG 3 cut(s) 44, 221, 308
NmuCI GTSAC 2 cut(s) 203, 590
OliI CACNNNNGTG 1 cut(s) 588
PagI TCATGA 1 cut(s) 40
PctI GAATGC 1 cut(s) 382
PdmI GAANNNNTTC 1 cut(s) 648
PfeI GAWTC 1 cut(s) 533
PkrI GCNGC 1 cut(s) 626
PleI GAGTC 3 cut(s) 45, 84, 652
PpsI GAGTC 3 cut(s) 45, 84, 652
PsrI GAACNNNNNNTAC 2 cut(s) 504, 536
PsuI RGATCY 1 cut(s) 526
RsaI GTAC 1 cut(s) 196
RsaNI GTAC 1 cut(s) 195
RseI CAYNNNNRTG 4 cut(s) 419, 473, 588, 666
SaqAI TTAA 3 cut(s) 32, 240, 417
SatI GCNGC 1 cut(s) 625
Sau3AI GATC 3 cut(s) 22, 526, 706
SchI GAGTC 3 cut(s) 46, 85, 652
SduI GDGCHC 1 cut(s) 570
SetI ASST 8 cut(s) 32, 64, 86, 212, 561, 582, 649, 699
SfaNI GCATC 1 cut(s) 420
SfcI CTRYAG 1 cut(s) 698
SmiMI CAYNNNNRTG 4 cut(s) 419, 473, 588, 666
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
Sse9I AATT 5 cut(s) 118, 151, 199, 312, 538
SsiI CCGC 1 cut(s) 473
SspI AATATT 1 cut(s) 516
SspMI CTAG 1 cut(s) 111
StyI CCWWGG 1 cut(s) 710
TaaI ACNGT 1 cut(s) 276
TaqI TCGA 1 cut(s) 612
TasI AATT 5 cut(s) 118, 151, 199, 312, 538
TatI WGTACW 1 cut(s) 194
TfiI GAWTC 1 cut(s) 533
Tru1I TTAA 3 cut(s) 32, 240, 417
Tru9I TTAA 3 cut(s) 32, 240, 417
TscAI CASTG 2 cut(s) 78, 281
TseFI GTSAC 2 cut(s) 203, 590
TseI GCWGC 1 cut(s) 624
Tsp45I GTSAC 2 cut(s) 203, 590
TspDTI ATGAA 3 cut(s) 60, 158, 490
TspRI CASTG 2 cut(s) 78, 281
XapI RAATTY 1 cut(s) 538
XmnI GAANNNNTTC 1 cut(s) 648
XspI CTAG 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.