RLG00000019471

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001282
Physical Location & Seq
Reverse (-)
52422163 .. 52422880
718 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019471

Sequence Viewer

Length: 552 bp
ATGGAGAGCCAGAAGAAGATAATGGTTGTTTTTGGGTTAGTTGCGGCTGTGTTTTTACAGTGTGTGGCGGCACAGACGGTCCATGTGGTGGGAGATAGCGTAGGCTGGATCGTACCACCGAACGGTGCACAACAGTATGTCACATGGGCGTCTGGTCAGAAGTTCATAGTTGGTGATTTTCTGATTTTCAACTTCGCGACAAACTCACACGACGTTCTGGAAGTACCCAAAGCATCGTTCGACTCTTGCAGCTCTAACATCCCAATCGGCAGCACCATCAAGACCGGCCCGGCCAACATCACTCTCAACTCCACCGGCGAACACCACTACATCTGTACTATCCCCAACCACTGCCAGTCCGGCCAGAAGCTCGCCATCACCGTCTTAGCATCCGCCACGTCACCCGGAGGCTCTCCTTCTGCACCATCTCCATCTACCACAGCTAACAACACGCCGGGGGTGACCGCGGTGCCGCCGAGCTCTTCATCCCGGGCTGCTGGGGCTGGTTTCGTTTTGTGCTCGTTGTCCCTTGTAATGGGATTGTTCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

184

Amino Acids

18.85

Weight (kDa)

6.94

Isoelectric Point (pI)

41.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 36 - 122 1.2e-24 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 469
AccB7I CCANNNNNTGG 1 cut(s) 88
AccII CGCG 2 cut(s) 197, 467
AciI CCGC 6 cut(s) 44, 68, 393, 465, 467, 473
AclWI GGATC 1 cut(s) 116
AcoI YGGCCR 2 cut(s) 291, 361
AcyI GRCGYC 1 cut(s) 149
AfaI GTAC 3 cut(s) 114, 225, 337
AfiI CCNNNNNNNGG 3 cut(s) 88, 122, 535
AgsI TTSAA 1 cut(s) 190
AjiI CACGTC 1 cut(s) 399
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AluBI AGCT 4 cut(s) 252, 370, 443, 480
AluI AGCT 4 cut(s) 252, 370, 443, 480
Alw21I GWGCWC 3 cut(s) 130, 482, 521
Alw44I GTGCAC 1 cut(s) 126
AlwI GGATC 1 cut(s) 116
Ama87I CYCGRG 1 cut(s) 489
AoxI GGCC 3 cut(s) 286, 291, 361
ApaLI GTGCAC 1 cut(s) 126
ApeKI GCWGC 3 cut(s) 249, 270, 494
AspS9I GGNCC 2 cut(s) 79, 287
AsuC2I CCSGG 5 cut(s) 290, 405, 456, 490, 491
AsuHPI GGTGA 4 cut(s) 185, 370, 393, 472
AvaI CYCGRG 1 cut(s) 489
AvaII GGWCC 1 cut(s) 79
BaeGI GKGCMC 1 cut(s) 130
BanI GGYRCC 1 cut(s) 469
BanII GRGCYC 1 cut(s) 482
Bbv12I GWGCWC 3 cut(s) 130, 482, 521
BbvI GCAGC 3 cut(s) 261, 282, 481
BccI CCATC 4 cut(s) 284, 383, 433, 439
BcnI CCSGG 5 cut(s) 290, 405, 456, 490, 491
BglI GCCNNNNNGGC 1 cut(s) 360
BisI GCNGC 6 cut(s) 45, 69, 250, 271, 473, 495
BlsI GCNGC 6 cut(s) 46, 70, 251, 272, 474, 496
Bme1390I CCNGG 5 cut(s) 290, 405, 456, 490, 491
Bme18I GGWCC 1 cut(s) 79
BmeT110I CYCGRG 1 cut(s) 489
BmgBI CACGTC 1 cut(s) 399
BmgT120I GGNCC 2 cut(s) 79, 287
BmiI GGNNCC 1 cut(s) 471
BmrFI CCNGG 5 cut(s) 290, 405, 456, 490, 491
BmsI GCATC 2 cut(s) 242, 398
BpuMI CCSGG 5 cut(s) 290, 405, 456, 490, 491
BsaHI GRCGYC 1 cut(s) 149
BsaJI CCNNGG 3 cut(s) 455, 465, 489
BsaXI ACNNNNNCTCC 2 cut(s) 84, 114
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 122, 535
Bse118I RCCGGY 2 cut(s) 284, 314
Bse1I ACTGG 1 cut(s) 355
BseDI CCNNGG 3 cut(s) 455, 465, 489
BseGI GGATG 3 cut(s) 258, 389, 485
BseLI CCNNNNNNNGG 3 cut(s) 88, 122, 535
BseNI ACTGG 1 cut(s) 355
BseSI GKGCMC 1 cut(s) 130
BseXI GCAGC 3 cut(s) 261, 282, 481
BseYI CCCAGC 1 cut(s) 497
BsgI GTGCAG 1 cut(s) 405
Bsh1236I CGCG 2 cut(s) 197, 467
BshFI GGCC 3 cut(s) 288, 293, 363
BshNI GGYRCC 1 cut(s) 469
BsiHKAI GWGCWC 3 cut(s) 130, 482, 521
BsiHKCI CYCGRG 1 cut(s) 489
BsiSI CCGG 7 cut(s) 285, 290, 315, 360, 405, 455, 490
BslFI GGGAC 1 cut(s) 511
BslI CCNNNNNNNGG 3 cut(s) 88, 122, 535
BsmFI GGGAC 1 cut(s) 511
BsnI GGCC 3 cut(s) 288, 293, 363
BsoBI CYCGRG 1 cut(s) 489
Bsp1286I GDGCHC 3 cut(s) 130, 482, 521
Bsp143I GATC 1 cut(s) 108
Bsp68I TCGCGA 1 cut(s) 197
BspACI CCGC 6 cut(s) 44, 68, 393, 465, 467, 473
BspANI GGCC 3 cut(s) 288, 293, 363
BspFNI CGCG 2 cut(s) 197, 467
BspLI GGNNCC 1 cut(s) 471
BspPI GGATC 1 cut(s) 116
BspQI GCTCTTC 1 cut(s) 487
BspT107I GGYRCC 1 cut(s) 469
BsrFI RCCGGY 2 cut(s) 284, 314
BsrI ACTGG 1 cut(s) 355
BssAI RCCGGY 2 cut(s) 284, 314
BssECI CCNNGG 3 cut(s) 455, 465, 489
BssMI GATC 1 cut(s) 108
BssNI GRCGYC 1 cut(s) 149
Bst4CI ACNGT 5 cut(s) 60, 79, 125, 135, 382
Bst6I CTCTTC 1 cut(s) 487
BstACI GRCGYC 1 cut(s) 149
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 1 cut(s) 385
BstDSI CCRYGG 1 cut(s) 465
BstEII GGTNACC 1 cut(s) 460
BstF5I GGATG 3 cut(s) 258, 389, 485
BstFNI CGCG 2 cut(s) 197, 467
BstKTI GATC 1 cut(s) 111
BstMBI GATC 1 cut(s) 108
BstMWI GCNNNNNNNGC 2 cut(s) 360, 500
BstPI GGTNACC 1 cut(s) 460
BstSCI CCNGG 5 cut(s) 288, 403, 454, 488, 489
BstSLI GKGCMC 1 cut(s) 130
BstUI CGCG 2 cut(s) 197, 467
BstV1I GCAGC 3 cut(s) 261, 282, 481
BsuRI GGCC 3 cut(s) 288, 293, 363
BtgI CCRYGG 1 cut(s) 465
BtrI CACGTC 1 cut(s) 399
BtsCI GGATG 3 cut(s) 258, 389, 485
BtsI GCAGTG 1 cut(s) 349
BtsIMutI CAGTG 2 cut(s) 65, 349
BtuMI TCGCGA 1 cut(s) 197
Cac8I GCNNGC 1 cut(s) 372
Cfr10I RCCGGY 2 cut(s) 284, 314
Cfr13I GGNCC 2 cut(s) 79, 287
Cfr42I CCGCGG 1 cut(s) 468
Cfr9I CCCGGG 1 cut(s) 489
CseI GACGC 1 cut(s) 138
Csp6I GTAC 3 cut(s) 113, 224, 336
CviAII CATG 2 cut(s) 83, 144
CviQI GTAC 3 cut(s) 113, 224, 336
DdeI CTNAG 1 cut(s) 385
DpnI GATC 1 cut(s) 110
DpnII GATC 1 cut(s) 108
EaeI YGGCCR 2 cut(s) 291, 361
Eam1104I CTCTTC 1 cut(s) 487
EarI CTCTTC 1 cut(s) 487
EciI GGCGGA 1 cut(s) 382
Ecl136II GAGCTC 1 cut(s) 480
Eco24I GRGCYC 1 cut(s) 482
Eco47I GGWCC 1 cut(s) 79
Eco53kI GAGCTC 1 cut(s) 480
Eco88I CYCGRG 1 cut(s) 489
Eco91I GGTNACC 1 cut(s) 460
EcoICRI GAGCTC 1 cut(s) 480
EcoO65I GGTNACC 1 cut(s) 460
EcoT38I GRGCYC 1 cut(s) 482
FaeI CATG 2 cut(s) 86, 147
FaiI YATR 4 cut(s) 84, 138, 145, 167
FaqI GGGAC 1 cut(s) 511
FatI CATG 2 cut(s) 82, 143
Fnu4HI GCNGC 6 cut(s) 45, 69, 250, 271, 473, 495
FokI GGATG 3 cut(s) 245, 376, 472
FriOI GRGCYC 1 cut(s) 482
Fsp4HI GCNGC 6 cut(s) 45, 69, 250, 271, 473, 495
GluI GCNGC 6 cut(s) 45, 69, 250, 271, 473, 495
GsaI CCCAGC 1 cut(s) 501
HaeIII GGCC 3 cut(s) 288, 293, 363
HapII CCGG 7 cut(s) 285, 290, 315, 360, 405, 455, 490
HgaI GACGC 1 cut(s) 138
Hin1I GRCGYC 1 cut(s) 149
Hin1II CATG 2 cut(s) 86, 147
HinfI GANTC 1 cut(s) 242
HpaII CCGG 7 cut(s) 285, 290, 315, 360, 405, 455, 490
HphI GGTGA 4 cut(s) 185, 370, 393, 472
Hpy166II GTNNAC 1 cut(s) 128
Hpy188I TCNGA 2 cut(s) 159, 183
Hpy188III TCNNGA 3 cut(s) 196, 218, 280
Hpy8I GTNNAC 1 cut(s) 128
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 1 cut(s) 426
HpyCH4III ACNGT 5 cut(s) 60, 79, 125, 135, 382
HpyCH4IV ACGT 2 cut(s) 213, 398
HpyCH4V TGCA 3 cut(s) 128, 249, 422
HpyF10VI GCNNNNNNNGC 2 cut(s) 360, 500
HpyF3I CTNAG 1 cut(s) 385
HpySE526I ACGT 2 cut(s) 213, 398
Hsp92I GRCGYC 1 cut(s) 149
Hsp92II CATG 2 cut(s) 86, 147
KspI CCGCGG 1 cut(s) 468
Kzo9I GATC 1 cut(s) 108
LguI GCTCTTC 1 cut(s) 487
Lsp1109I GCAGC 3 cut(s) 261, 282, 481
LweI GCATC 2 cut(s) 242, 398
MaeII ACGT 2 cut(s) 213, 398
MaeIII GTNAC 3 cut(s) 139, 399, 460
MalI GATC 1 cut(s) 110
MboI GATC 1 cut(s) 108
MboII GAAGA 3 cut(s) 25, 28, 474
MhlI GDGCHC 3 cut(s) 130, 482, 521
MlyI GAGTC 1 cut(s) 236
MnlI CCTC 1 cut(s) 401
MspA1I CMGCKG 1 cut(s) 467
MspI CCGG 7 cut(s) 285, 290, 315, 360, 405, 455, 490
MspR9I CCNGG 5 cut(s) 290, 405, 456, 490, 491
MvnI CGCG 2 cut(s) 197, 467
MwoI GCNNNNNNNGC 2 cut(s) 360, 500
NciI CCSGG 5 cut(s) 290, 405, 456, 490, 491
NdeII GATC 1 cut(s) 108
NlaIII CATG 2 cut(s) 86, 147
NlaIV GGNNCC 1 cut(s) 471
NmeAIII GCCGAG 1 cut(s) 501
NmuCI GTSAC 3 cut(s) 139, 399, 460
NruI TCGCGA 1 cut(s) 197
PciSI GCTCTTC 1 cut(s) 487
PcsI WCGNNNNNNNCGW 1 cut(s) 378
PflMI CCANNNNNTGG 1 cut(s) 88
PkrI GCNGC 6 cut(s) 46, 70, 251, 272, 474, 496
PleI GAGTC 1 cut(s) 236
PpsI GAGTC 1 cut(s) 236
Psp124BI GAGCTC 1 cut(s) 482
PspEI GGTNACC 1 cut(s) 460
PspFI CCCAGC 1 cut(s) 497
PspN4I GGNNCC 1 cut(s) 471
PspPI GGNCC 2 cut(s) 79, 287
RruI TCGCGA 1 cut(s) 197
RsaI GTAC 3 cut(s) 114, 225, 337
RsaNI GTAC 3 cut(s) 113, 224, 336
SacI GAGCTC 1 cut(s) 482
SacII CCGCGG 1 cut(s) 468
SapI GCTCTTC 1 cut(s) 487
SatI GCNGC 6 cut(s) 45, 69, 250, 271, 473, 495
Sau3AI GATC 1 cut(s) 108
Sau96I GGNCC 2 cut(s) 79, 287
SchI GAGTC 1 cut(s) 236
ScrFI CCNGG 5 cut(s) 290, 405, 456, 490, 491
SduI GDGCHC 3 cut(s) 130, 482, 521
SetI ASST 6 cut(s) 216, 254, 372, 401, 445, 482
SfaNI GCATC 2 cut(s) 242, 398
Sfr303I CCGCGG 1 cut(s) 468
SgrAI CRCCGGYG 1 cut(s) 314
SgrBI CCGCGG 1 cut(s) 468
SinI GGWCC 1 cut(s) 79
SmaI CCCGGG 1 cut(s) 491
SsiI CCGC 6 cut(s) 44, 68, 393, 465, 467, 473
SstI GAGCTC 1 cut(s) 482
StyD4I CCNGG 5 cut(s) 288, 403, 454, 488, 489
TaaI ACNGT 5 cut(s) 60, 79, 125, 135, 382
TaiI ACGT 2 cut(s) 216, 401
TaqI TCGA 1 cut(s) 240
TatI WGTACW 1 cut(s) 335
TauI GCSGC 3 cut(s) 47, 71, 475
TscAI CASTG 2 cut(s) 65, 356
TseFI GTSAC 3 cut(s) 139, 399, 460
TseI GCWGC 3 cut(s) 249, 270, 494
Tsp45I GTSAC 3 cut(s) 139, 399, 460
TspDTI ATGAA 2 cut(s) 154, 474
TspMI CCCGGG 1 cut(s) 489
TspRI CASTG 2 cut(s) 65, 356
Van91I CCANNNNNTGG 1 cut(s) 88
VneI GTGCAC 1 cut(s) 126
VpaK11BI GGWCC 1 cut(s) 79
XmaI CCCGGG 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.