Rh1BG222500

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0001282
Physical Location & Seq
Reverse (-)
34157204 .. 34167302
10099 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG222500.1

Sequence Viewer

Length: 1407 bp
ATGTCGTCGTCGTCGTCATCGGAGGAGGAAGCAGACTCTGAATCTCCGATCAGCATGTCATCGTCTTCGTCGTCGTCGTCGGCCTCATCGGAGAAGAAGGAAACTCTGTTGTTTCATGTGGAGCTGAACGAAGTCCGTCTTCTCCAGAAGGCCTTGAGACCCCTGTGTGAGACTGCCTACTTCCCGGACGACATTTCCCTCGCCGGAAAGATCCGCGACTCCGGACACTTCTGCATATCTCCGGACGGGGTTCTTATCGCCTCCACAACCTCTTTCCCGCCTCATCTTGACGCCTATCTCGAACTGCCGCGTGAGACCTTCGCCGCCCTTCGCTGCCACAAGGACCATGTGCTCCGCCTCAACCTCCGCCTCTTGGAGGACCGAATCGCCGCCTCAACCACCGTCAACGGTTGTGATGGTGTTTCTGCATGCTTCTCCGGAGAGAAGTACAGGCACAGCGCCTTGCTCTATTCTCTGAGGGACAAGGAAGGTTTTGCCGTGCACAGCTCTGAGATACCGCTCATTTGCTCCTATCCCGGTTTTCTGGCGACATTAGATTGGTCAGATTACAGTTATCAACTCAGCATTGAGATGCCGACGGAGACTTTTAAGCAACTCATCAATTCTCTGAGCTTGTATGGGCTTGCAGTTCGTGCCACCGTGACAATTACTCAAATCATGTTCCGTGTAGTCAATGAGGAGGTTGTTCTGAGGGCAGAGGCTGAGGTCTACAAGATTCTGCGTTCGAGAAGTCGATATCCGTATTTGTTGGAATTTGGTCTCCATCAAAAGAGTGCATTCCTGAATGCGGCGAGTCTATCAGACAGAGTTAGGCTTCACCAGCTTTTGGATTTACGCACTGTGTTGGAGATATCAGTAGTGCTCACATACAATTCCATTAGAAGAGAAGAAGAGATCAAGAAAGAAGGAGAAGCCAGAGAGATCGAGATGGAGAGCCAGAAGAAGATGATTGTTGTTTTTGGGTTAGTTGCTGCTGTGTTTTTACAGCGTGTGGCGGCGGAGACATCGCATGTGGTGGGAGATAGCGTAGGCTGGACCATACCACAGAACGGCGCACAAGAGTATGTCACATGGGCGTCTGGTCAGAAGTTCGTAGTTGGTGATGTTCTGGTTTTCAGCTTCGCGACAAGCACACACGACGTGGTGGAAGTACCCAAAGCATCCTTCGACTCTTGCAGCTCCACCAACCCAATCGGCAGCACCATCACCACCGGCCCGGCCAACATCACTCTCACCTCCTCCGGCGAACACTACTACATCTGTACTTTCGGCCTCCACTGCGAGTCCGGCCAGAAGCTCGCCATCACCGTCTCAGCATCCGCCACATCACCCGGAGCCTCCCCTTCTGCACCATCTCTATCTACCAACCCTGACAACACGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

468

Amino Acids

51.31

Weight (kDa)

5.38

Isoelectric Point (pI)

50.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 352 - 438 1.6e-24 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 847
AccBSI CCGCTC 1 cut(s) 520
AccI GTMKAC 1 cut(s) 729
AccII CGCG 3 cut(s) 216, 310, 1145
AccIII TCCGGA 3 cut(s) 221, 241, 437
AclWI GGATC 1 cut(s) 205
AcoI YGGCCR 2 cut(s) 1239, 1309
AcsI RAATTY 1 cut(s) 773
AcyI GRCGYC 2 cut(s) 291, 1097
AdeI CACNNNGTG 1 cut(s) 1162
AfaI GTAC 4 cut(s) 449, 1173, 1285, 1403
AfiI CCNNNNNNNGG 5 cut(s) 373, 376, 808, 847, 1070
AflIII ACRYGT 1 cut(s) 1398
AjiI CACGTC 1 cut(s) 1162
AjuI GAANNNNNNNTTGG 2 cut(s) 1169, 1201
AluBI AGCT 7 cut(s) 124, 507, 633, 844, 1140, 1200, 1318
AluI AGCT 7 cut(s) 124, 507, 633, 844, 1140, 1200, 1318
Alw21I GWGCWC 3 cut(s) 354, 504, 885
Alw26I GTCTC 7 cut(s) 151, 164, 308, 596, 785, 1016, 1336
Alw44I GTGCAC 1 cut(s) 500
AlwI GGATC 1 cut(s) 205
AlwNI CAGNNNCTG 2 cut(s) 38, 722
Aor13HI TCCGGA 3 cut(s) 221, 241, 437
AoxI GGCC 6 cut(s) 81, 150, 1234, 1239, 1291, 1309
ApaLI GTGCAC 1 cut(s) 500
ApeKI GCWGC 4 cut(s) 333, 992, 1197, 1218
ApoI RAATTY 1 cut(s) 773
AspLEI GCGC 2 cut(s) 461, 1076
AspS9I GGNCC 4 cut(s) 343, 379, 1056, 1235
AsuC2I CCSGG 4 cut(s) 185, 537, 1238, 1353
AsuHPI GGTGA 6 cut(s) 830, 1133, 1219, 1246, 1318, 1341
AvaII GGWCC 3 cut(s) 343, 379, 1056
BaeGI GKGCMC 1 cut(s) 504
BbsI GAAGAC 2 cut(s) 57, 131
Bbv12I GWGCWC 3 cut(s) 354, 504, 885
BbvCI CCTCAGC 1 cut(s) 723
BbvI GCAGC 4 cut(s) 320, 979, 1209, 1230
BccI CCATC 6 cut(s) 410, 792, 943, 1232, 1331, 1381
BceAI ACGGC 2 cut(s) 482, 1087
BcnI CCSGG 4 cut(s) 185, 537, 1238, 1353
BcoDI GTCTC 7 cut(s) 151, 164, 308, 596, 785, 1016, 1336
BfaI CTAG 1 cut(s) 1405
BfoI RGCGCY 1 cut(s) 462
BisI GCNGC 9 cut(s) 308, 324, 334, 390, 810, 993, 1017, 1198, 1219
BlsI GCNGC 9 cut(s) 309, 325, 335, 391, 811, 994, 1018, 1199, 1220
Bme1390I CCNGG 4 cut(s) 185, 537, 1238, 1353
Bme18I GGWCC 3 cut(s) 343, 379, 1056
BmgBI CACGTC 1 cut(s) 1162
BmgT120I GGNCC 4 cut(s) 343, 379, 1056, 1235
BmiI GGNNCC 1 cut(s) 1357
BmrFI CCNGG 4 cut(s) 185, 537, 1238, 1353
BmsI GCATC 3 cut(s) 582, 1190, 1346
BpiI GAAGAC 2 cut(s) 57, 131
BpmI CTGGAG 1 cut(s) 128
Bpu10I CCTNAGC 1 cut(s) 723
BpuEI CTTGAG 1 cut(s) 175
BpuMI CCSGG 4 cut(s) 185, 537, 1238, 1353
BsaAI YACGTR 1 cut(s) 1401
BsaHI GRCGYC 2 cut(s) 291, 1097
BsaI GGTCTC 3 cut(s) 151, 308, 785
BsaWI WCCGGW 3 cut(s) 221, 241, 437
BsaXI ACNNNNNCTCC 2 cut(s) 1032, 1062
Bsc4I CCNNNNNNNGG 5 cut(s) 373, 376, 808, 847, 1070
Bse118I RCCGGY 1 cut(s) 1232
BseAI TCCGGA 3 cut(s) 221, 241, 437
BseGI GGATG 2 cut(s) 1181, 1337
BseLI CCNNNNNNNGG 5 cut(s) 373, 376, 808, 847, 1070
BseMII CTCAG 7 cut(s) 467, 501, 595, 620, 701, 714, 1347
BseRI GAGGAG 3 cut(s) 38, 713, 1249
BseSI GKGCMC 1 cut(s) 504
BseXI GCAGC 4 cut(s) 320, 979, 1209, 1230
BsgI GTGCAG 1 cut(s) 1353
Bsh1236I CGCG 3 cut(s) 216, 310, 1145
BshFI GGCC 6 cut(s) 83, 152, 1236, 1241, 1293, 1311
BsiHKAI GWGCWC 3 cut(s) 354, 504, 885
BslFI GGGAC 1 cut(s) 494
BslI CCNNNNNNNGG 5 cut(s) 373, 376, 808, 847, 1070
BsmAI GTCTC 7 cut(s) 151, 164, 308, 596, 785, 1016, 1336
BsmBI CGTCTC 1 cut(s) 1336
BsmFI GGGAC 1 cut(s) 494
BsmI GAATGC 2 cut(s) 797, 811
BsnI GGCC 6 cut(s) 83, 152, 1236, 1241, 1293, 1311
Bso31I GGTCTC 3 cut(s) 151, 308, 785
Bsp1286I GDGCHC 3 cut(s) 354, 504, 885
Bsp13I TCCGGA 3 cut(s) 221, 241, 437
Bsp143I GATC 4 cut(s) 48, 210, 915, 942
Bsp68I TCGCGA 1 cut(s) 1145
BspANI GGCC 6 cut(s) 83, 152, 1236, 1241, 1293, 1311
BspCNI CTCAG 7 cut(s) 468, 502, 594, 621, 702, 715, 1346
BspEI TCCGGA 3 cut(s) 221, 241, 437
BspFNI CGCG 3 cut(s) 216, 310, 1145
BspLI GGNNCC 1 cut(s) 1357
BspPI GGATC 1 cut(s) 205
BspTNI GGTCTC 3 cut(s) 151, 308, 785
BsrBI CCGCTC 1 cut(s) 520
BsrFI RCCGGY 1 cut(s) 1232
BssAI RCCGGY 1 cut(s) 1232
BssMI GATC 4 cut(s) 48, 210, 915, 942
BssNI GRCGYC 2 cut(s) 291, 1097
Bst4CI ACNGT 6 cut(s) 403, 410, 572, 661, 862, 1330
Bst6I CTCTTC 2 cut(s) 898, 906
BstACI GRCGYC 2 cut(s) 291, 1097
BstAPI GCANNNNNTGC 1 cut(s) 653
BstBAI YACGTR 1 cut(s) 1401
BstC8I GCNNGC 3 cut(s) 430, 645, 1320
BstDEI CTNAG 7 cut(s) 476, 510, 581, 629, 710, 723, 1333
BstENI CCTNNNNNAGG 1 cut(s) 374
BstF5I GGATG 2 cut(s) 1181, 1337
BstFNI CGCG 3 cut(s) 216, 310, 1145
BstH2I RGCGCY 1 cut(s) 462
BstHHI GCGC 2 cut(s) 461, 1076
BstKTI GATC 4 cut(s) 51, 213, 918, 945
BstMAI GTCTC 7 cut(s) 151, 164, 308, 596, 785, 1016, 1336
BstMBI GATC 4 cut(s) 48, 210, 915, 942
BstMWI GCNNNNNNNGC 4 cut(s) 653, 841, 1299, 1308
BstNSI RCATGY 3 cut(s) 58, 432, 1034
BstSCI CCNGG 4 cut(s) 183, 535, 1236, 1351
BstSLI GKGCMC 1 cut(s) 504
BstUI CGCG 3 cut(s) 216, 310, 1145
BstV1I GCAGC 4 cut(s) 320, 979, 1209, 1230
BstV2I GAAGAC 2 cut(s) 57, 131
BstX2I RGATCY 1 cut(s) 210
BstYI RGATCY 1 cut(s) 210
BsuRI GGCC 6 cut(s) 83, 152, 1236, 1241, 1293, 1311
BtgZI GCGATG 1 cut(s) 1011
BtrI CACGTC 1 cut(s) 1162
BtsCI GGATG 2 cut(s) 1181, 1337
BtsI GCAGTG 1 cut(s) 1297
BtsIMutI CAGTG 2 cut(s) 858, 1297
BtuMI TCGCGA 1 cut(s) 1145
Cac8I GCNNGC 3 cut(s) 430, 645, 1320
CaiI CAGNNNCTG 2 cut(s) 38, 722
CfoI GCGC 2 cut(s) 461, 1076
Cfr10I RCCGGY 1 cut(s) 1232
Cfr13I GGNCC 4 cut(s) 343, 379, 1056, 1235
CseI GACGC 2 cut(s) 299, 1086
Csp6I GTAC 4 cut(s) 448, 1172, 1284, 1402
CviAII CATG 7 cut(s) 55, 116, 347, 429, 679, 1031, 1092
CviQI GTAC 4 cut(s) 448, 1172, 1284, 1402
DdeI CTNAG 7 cut(s) 476, 510, 581, 629, 710, 723, 1333
DpnI GATC 4 cut(s) 50, 212, 917, 944
DpnII GATC 4 cut(s) 48, 210, 915, 942
DraIII CACNNNGTG 1 cut(s) 1162
EaeI YGGCCR 2 cut(s) 1239, 1309
Eam1104I CTCTTC 2 cut(s) 898, 906
EarI CTCTTC 2 cut(s) 898, 906
EciI GGCGGA 4 cut(s) 344, 356, 1034, 1330
Eco147I AGGCCT 1 cut(s) 152
Eco31I GGTCTC 3 cut(s) 151, 308, 785
Eco32I GATATC 2 cut(s) 758, 873
Eco47I GGWCC 3 cut(s) 343, 379, 1056
EcoNI CCTNNNNNAGG 1 cut(s) 374
EcoRV GATATC 2 cut(s) 758, 873
Esp3I CGTCTC 1 cut(s) 1336
FaeI CATG 7 cut(s) 58, 119, 350, 432, 682, 1034, 1095
FalI AAGNNNNNCTT 2 cut(s) 123, 155
FaqI GGGAC 1 cut(s) 494
FatI CATG 7 cut(s) 54, 115, 346, 428, 678, 1030, 1091
FauI CCCGC 1 cut(s) 285
FblI GTMKAC 1 cut(s) 729
Fnu4HI GCNGC 9 cut(s) 308, 324, 334, 390, 810, 993, 1017, 1198, 1219
FokI GGATG 2 cut(s) 1168, 1324
Fsp4HI GCNGC 9 cut(s) 308, 324, 334, 390, 810, 993, 1017, 1198, 1219
FspBI CTAG 1 cut(s) 1405
GlaI GCGC 2 cut(s) 460, 1075
GluI GCNGC 9 cut(s) 308, 324, 334, 390, 810, 993, 1017, 1198, 1219
GsuI CTGGAG 1 cut(s) 128
HaeII RGCGCY 1 cut(s) 462
HaeIII GGCC 6 cut(s) 83, 152, 1236, 1241, 1293, 1311
HgaI GACGC 2 cut(s) 299, 1086
HhaI GCGC 2 cut(s) 461, 1076
Hin1I GRCGYC 2 cut(s) 291, 1097
Hin1II CATG 7 cut(s) 58, 119, 350, 432, 682, 1034, 1095
Hin6I GCGC 2 cut(s) 459, 1074
HinP1I GCGC 2 cut(s) 459, 1074
HincII GTYRAC 1 cut(s) 406
HindII GTYRAC 1 cut(s) 406
HinfI GANTC 8 cut(s) 35, 41, 218, 384, 736, 814, 1190, 1304
HphI GGTGA 6 cut(s) 830, 1133, 1219, 1246, 1318, 1341
Hpy166II GTNNAC 3 cut(s) 406, 502, 730
Hpy8I GTNNAC 3 cut(s) 406, 502, 730
Hpy99I CGWCG 9 cut(s) 10, 13, 16, 73, 76, 79, 82, 601, 1163
HpyAV CCTTC 8 cut(s) 91, 142, 328, 338, 482, 920, 1195, 1374
HpyCH4III ACNGT 6 cut(s) 403, 410, 572, 661, 862, 1330
HpyCH4IV ACGT 2 cut(s) 1161, 1400
HpyCH4V TGCA 7 cut(s) 234, 428, 502, 647, 797, 1197, 1370
HpyF10VI GCNNNNNNNGC 4 cut(s) 653, 841, 1299, 1308
HpyF3I CTNAG 7 cut(s) 476, 510, 581, 629, 710, 723, 1333
HpySE526I ACGT 2 cut(s) 1161, 1400
Hsp92I GRCGYC 2 cut(s) 291, 1097
Hsp92II CATG 7 cut(s) 58, 119, 350, 432, 682, 1034, 1095
HspAI GCGC 2 cut(s) 459, 1074
Kpn2I TCCGGA 3 cut(s) 221, 241, 437
Kzo9I GATC 4 cut(s) 48, 210, 915, 942
LmnI GCTCC 5 cut(s) 121, 357, 533, 1205, 1355
Lsp1109I GCAGC 4 cut(s) 320, 979, 1209, 1230
LweI GCATC 3 cut(s) 582, 1190, 1346
MaeI CTAG 1 cut(s) 1405
MaeII ACGT 2 cut(s) 1161, 1400
MaeIII GTNAC 2 cut(s) 661, 1087
MalI GATC 4 cut(s) 50, 212, 917, 944
MbiI CCGCTC 1 cut(s) 520
MboI GATC 4 cut(s) 48, 210, 915, 942
MboII GAAGA 8 cut(s) 57, 106, 131, 915, 920, 923, 973, 976
MflI RGATCY 1 cut(s) 210
MhlI GDGCHC 3 cut(s) 354, 504, 885
MluCI AATT 4 cut(s) 622, 666, 773, 892
MlyI GAGTC 5 cut(s) 29, 212, 823, 1184, 1313
MmeI TCCRAC 2 cut(s) 750, 846
MroI TCCGGA 3 cut(s) 221, 241, 437
MseI TTAA 1 cut(s) 609
MslI CAYNNNNRTG 1 cut(s) 590
MspR9I CCNGG 4 cut(s) 185, 537, 1238, 1353
Mva1269I GAATGC 2 cut(s) 797, 811
MvnI CGCG 3 cut(s) 216, 310, 1145
MwoI GCNNNNNNNGC 4 cut(s) 653, 841, 1299, 1308
NciI CCSGG 4 cut(s) 185, 537, 1238, 1353
NdeII GATC 4 cut(s) 48, 210, 915, 942
NlaIII CATG 7 cut(s) 58, 119, 350, 432, 682, 1034, 1095
NlaIV GGNNCC 1 cut(s) 1357
NmuCI GTSAC 2 cut(s) 661, 1087
NruI TCGCGA 1 cut(s) 1145
NspI RCATGY 3 cut(s) 58, 432, 1034
PaeI GCATGC 1 cut(s) 432
PceI AGGCCT 1 cut(s) 152
PcsI WCGNNNNNNNCGW 5 cut(s) 11, 68, 74, 297, 1326
PctI GAATGC 2 cut(s) 797, 811
PfeI GAWTC 3 cut(s) 41, 384, 736
PflMI CCANNNNNTGG 1 cut(s) 847
PfoI TCCNGGA 1 cut(s) 183
PkrI GCNGC 9 cut(s) 309, 325, 335, 391, 811, 994, 1018, 1199, 1220
PleI GAGTC 5 cut(s) 29, 212, 822, 1184, 1312
PpsI GAGTC 5 cut(s) 29, 212, 822, 1184, 1312
Ppu21I YACGTR 1 cut(s) 1401
PspN4I GGNNCC 1 cut(s) 1357
PspPI GGNCC 4 cut(s) 343, 379, 1056, 1235
PstNI CAGNNNCTG 2 cut(s) 38, 722
PsuI RGATCY 1 cut(s) 210
RruI TCGCGA 1 cut(s) 1145
RsaI GTAC 4 cut(s) 449, 1173, 1285, 1403
RsaNI GTAC 4 cut(s) 448, 1172, 1284, 1402
RseI CAYNNNNRTG 1 cut(s) 590
SaqAI TTAA 1 cut(s) 609
SatI GCNGC 9 cut(s) 308, 324, 334, 390, 810, 993, 1017, 1198, 1219
Sau3AI GATC 4 cut(s) 48, 210, 915, 942
Sau96I GGNCC 4 cut(s) 343, 379, 1056, 1235
SchI GAGTC 5 cut(s) 29, 212, 823, 1184, 1313
ScrFI CCNGG 4 cut(s) 185, 537, 1238, 1353
SduI GDGCHC 3 cut(s) 354, 504, 885
SfaNI GCATC 3 cut(s) 582, 1190, 1346
SinI GGWCC 3 cut(s) 343, 379, 1056
SmiMI CAYNNNNRTG 1 cut(s) 590
SmlI CTYRAG 1 cut(s) 154
SmoI CTYRAG 1 cut(s) 154
SphI GCATGC 1 cut(s) 432
Sse9I AATT 4 cut(s) 622, 666, 773, 892
SseBI AGGCCT 1 cut(s) 152
SspMI CTAG 1 cut(s) 1405
StuI AGGCCT 1 cut(s) 152
StyD4I CCNGG 4 cut(s) 183, 535, 1236, 1351
TaaI ACNGT 6 cut(s) 403, 410, 572, 661, 862, 1330
TaiI ACGT 2 cut(s) 1164, 1403
TaqI TCGA 5 cut(s) 300, 746, 754, 945, 1188
TaqII GACCGA 1 cut(s) 396
TasI AATT 4 cut(s) 622, 666, 773, 892
TatI WGTACW 2 cut(s) 447, 1283
TauI GCSGC 5 cut(s) 310, 326, 392, 812, 1019
TfiI GAWTC 3 cut(s) 41, 384, 736
Tru1I TTAA 1 cut(s) 609
Tru9I TTAA 1 cut(s) 609
TscAI CASTG 2 cut(s) 865, 1304
TseFI GTSAC 2 cut(s) 661, 1087
TseI GCWGC 4 cut(s) 333, 992, 1197, 1218
Tsp45I GTSAC 2 cut(s) 661, 1087
TspDTI ATGAA 1 cut(s) 104
TspGWI ACGGA 4 cut(s) 125, 614, 674, 750
TspRI CASTG 2 cut(s) 865, 1304
Van91I CCANNNNNTGG 1 cut(s) 847
VneI GTGCAC 1 cut(s) 500
VpaK11BI GGWCC 3 cut(s) 343, 379, 1056
XagI CCTNNNNNAGG 1 cut(s) 374
XapI RAATTY 1 cut(s) 773
XceI RCATGY 3 cut(s) 58, 432, 1034
XmiI GTMKAC 1 cut(s) 729
XspI CTAG 1 cut(s) 1405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.