MD17G1211100.v1.1

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001282
Physical Location & Seq
Forward (+)
25605523 .. 25606005
483 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1211100.v1.1.491

Sequence Viewer

Length: 483 bp
ATGTTTCTTAAATATTTTCCAGTTTCTAACTTCGCCACAAACGCACACGATGTACAAGAAGTACCCAAAGCATCATTCGACTCGTGTAGTTCGGAAAACCCAGTTAGCACAGCTATCACAACTGGCCCAGCAAACATCACTCCCACCTCTTCTAGCGACCACTACTACATTTGCACTTATGGCCGTCATTGCCAGTCGGGCCAGAAGCTAGCCATCACCGTCTCAGCTGCTTCCCCAGGCGCATCACCTTCTGTGCCACCACCGCCCTCTACCAGTAACACCCCTCCAACTACCCCTTCCCCCACATCAAATGACCCTGCTGCTTGCGCACCAGTCCCTGCTCTATCACCATCCATGAATGGGGGAGTACCCACTGTGAACATCACCCCGGGGTCTCAGCCTCCTCTGGATTCTTCTTCACCTGCTGTTTTGGCTAGTTTCACCTTGTCGGTCACGGCTCTTGTCGCAGGCTTGTTCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

161

Amino Acids

16.23

Weight (kDa)

5.3

Isoelectric Point (pI)

77.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 10 - 68 1.5e-10 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 430
Acc16I TGCGCA 1 cut(s) 328
Acc36I ACCTGC 1 cut(s) 430
AciI CCGC 1 cut(s) 263
AcoI YGGCCR 1 cut(s) 181
AfaI GTAC 3 cut(s) 54, 63, 369
AfiI CCNNNNNNNGG 1 cut(s) 360
AjnI CCWGG 1 cut(s) 235
AjuI GAANNNNNNNTTGG 4 cut(s) 59, 91, 280, 312
AluBI AGCT 3 cut(s) 113, 208, 227
AluI AGCT 3 cut(s) 113, 208, 227
Alw26I GTCTC 2 cut(s) 226, 399
AlwNI CAGNNNCTG 1 cut(s) 338
Ama87I CYCGRG 1 cut(s) 388
AoxI GGCC 3 cut(s) 124, 181, 199
ApeKI GCWGC 2 cut(s) 227, 320
AspLEI GCGC 2 cut(s) 242, 329
AspS9I GGNCC 2 cut(s) 125, 199
AsuC2I CCSGG 2 cut(s) 389, 390
AsuHPI GGTGA 6 cut(s) 208, 237, 339, 376, 411, 433
AsuNHI GCTAGC 1 cut(s) 208
AvaI CYCGRG 1 cut(s) 388
BauI CACGAG 1 cut(s) 82
BbvI GCAGC 2 cut(s) 214, 307
BccI CCATC 2 cut(s) 221, 358
BceAI ACGGC 2 cut(s) 168, 471
BciT130I CCWGG 1 cut(s) 237
BcnI CCSGG 2 cut(s) 389, 390
BcoDI GTCTC 2 cut(s) 226, 399
BfaI CTAG 3 cut(s) 153, 209, 435
BfuAI ACCTGC 1 cut(s) 430
BglI GCCNNNNNGGC 1 cut(s) 198
BisI GCNGC 2 cut(s) 228, 321
BlsI GCNGC 2 cut(s) 229, 322
Bme1390I CCNGG 3 cut(s) 237, 389, 390
BmeT110I CYCGRG 1 cut(s) 388
BmgT120I GGNCC 2 cut(s) 125, 199
BmrFI CCNGG 3 cut(s) 237, 389, 390
BmrI ACTGGG 1 cut(s) 95
BmsI GCATC 2 cut(s) 80, 251
BmtI GCTAGC 1 cut(s) 212
BmuI ACTGGG 1 cut(s) 95
BpuMI CCSGG 2 cut(s) 389, 390
BsaI GGTCTC 1 cut(s) 399
BsaJI CCNNGG 4 cut(s) 235, 387, 388, 389
Bsc4I CCNNNNNNNGG 1 cut(s) 360
Bse1I ACTGG 6 cut(s) 20, 101, 127, 193, 273, 332
Bse3DI GCAATG 1 cut(s) 187
BseBI CCWGG 1 cut(s) 237
BseDI CCNNGG 4 cut(s) 235, 387, 388, 389
BseGI GGATG 1 cut(s) 350
BseLI CCNNNNNNNGG 1 cut(s) 360
BseMI GCAATG 1 cut(s) 187
BseMII CTCAG 2 cut(s) 237, 410
BseNI ACTGG 6 cut(s) 20, 101, 127, 193, 273, 332
BseRI GAGGAG 1 cut(s) 393
BseXI GCAGC 2 cut(s) 214, 307
BseYI CCCAGC 1 cut(s) 127
BshFI GGCC 3 cut(s) 126, 183, 201
BsiHKCI CYCGRG 1 cut(s) 388
BsiSI CCGG 1 cut(s) 389
BslFI GGGAC 1 cut(s) 320
BslI CCNNNNNNNGG 1 cut(s) 360
BsmAI GTCTC 2 cut(s) 226, 399
BsmBI CGTCTC 1 cut(s) 226
BsmFI GGGAC 1 cut(s) 320
BsnI GGCC 3 cut(s) 126, 183, 201
Bso31I GGTCTC 1 cut(s) 399
BsoBI CYCGRG 1 cut(s) 388
Bsp1407I TGTACA 1 cut(s) 52
BspACI CCGC 1 cut(s) 263
BspANI GGCC 3 cut(s) 126, 183, 201
BspCNI CTCAG 2 cut(s) 236, 409
BspMI ACCTGC 1 cut(s) 430
BspOI GCTAGC 1 cut(s) 212
BspTNI GGTCTC 1 cut(s) 399
BsrDI GCAATG 1 cut(s) 187
BsrGI TGTACA 1 cut(s) 52
BsrI ACTGG 6 cut(s) 20, 101, 127, 193, 273, 332
BssECI CCNNGG 4 cut(s) 235, 387, 388, 389
BssSI CACGAG 1 cut(s) 82
Bst2BI CACGAG 1 cut(s) 82
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 2 cut(s) 220, 376
Bst6I CTCTTC 1 cut(s) 154
BstAUI TGTACA 1 cut(s) 52
BstC8I GCNNGC 3 cut(s) 210, 325, 469
BstDEI CTNAG 2 cut(s) 223, 396
BstF5I GGATG 1 cut(s) 350
BstHHI GCGC 2 cut(s) 242, 329
BstMAI GTCTC 2 cut(s) 226, 399
BstMWI GCNNNNNNNGC 8 cut(s) 41, 180, 189, 198, 262, 326, 431, 464
BstNI CCWGG 1 cut(s) 237
BstSCI CCNGG 3 cut(s) 235, 387, 388
BstV1I GCAGC 2 cut(s) 214, 307
BsuRI GGCC 3 cut(s) 126, 183, 201
BtsCI GGATG 1 cut(s) 350
BtsIMutI CAGTG 1 cut(s) 372
BveI ACCTGC 1 cut(s) 430
Cac8I GCNNGC 3 cut(s) 210, 325, 469
CaiI CAGNNNCTG 1 cut(s) 338
CfoI GCGC 2 cut(s) 242, 329
Cfr13I GGNCC 2 cut(s) 125, 199
Cfr9I CCCGGG 1 cut(s) 388
Csp6I GTAC 3 cut(s) 53, 62, 368
CviAII CATG 1 cut(s) 355
CviQI GTAC 3 cut(s) 53, 62, 368
DdeI CTNAG 2 cut(s) 223, 396
EaeI YGGCCR 1 cut(s) 181
Eam1104I CTCTTC 1 cut(s) 154
EarI CTCTTC 1 cut(s) 154
Eco31I GGTCTC 1 cut(s) 399
Eco88I CYCGRG 1 cut(s) 388
EcoRII CCWGG 1 cut(s) 235
Esp3I CGTCTC 1 cut(s) 226
FaeI CATG 1 cut(s) 358
FaiI YATR 2 cut(s) 180, 356
FaqI GGGAC 1 cut(s) 320
FatI CATG 1 cut(s) 354
Fnu4HI GCNGC 2 cut(s) 228, 321
FokI GGATG 1 cut(s) 337
Fsp4HI GCNGC 2 cut(s) 228, 321
FspBI CTAG 3 cut(s) 153, 209, 435
FspI TGCGCA 1 cut(s) 328
GlaI GCGC 2 cut(s) 241, 328
GluI GCNGC 2 cut(s) 228, 321
GsaI CCCAGC 1 cut(s) 131
HaeIII GGCC 3 cut(s) 126, 183, 201
HapII CCGG 1 cut(s) 389
HhaI GCGC 2 cut(s) 242, 329
Hin1II CATG 1 cut(s) 358
Hin6I GCGC 2 cut(s) 240, 327
HinP1I GCGC 2 cut(s) 240, 327
HinfI GANTC 2 cut(s) 80, 410
HpaII CCGG 1 cut(s) 389
HphI GGTGA 6 cut(s) 208, 237, 339, 376, 411, 433
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 1 cut(s) 94
Hpy188III TCNNGA 1 cut(s) 407
Hpy8I GTNNAC 1 cut(s) 379
HpyAV CCTTC 2 cut(s) 258, 306
HpyCH4III ACNGT 2 cut(s) 220, 376
HpyCH4V TGCA 1 cut(s) 174
HpyF10VI GCNNNNNNNGC 8 cut(s) 41, 180, 189, 198, 262, 326, 431, 464
HpyF3I CTNAG 2 cut(s) 223, 396
Hsp92II CATG 1 cut(s) 358
HspAI GCGC 2 cut(s) 240, 327
Lsp1109I GCAGC 2 cut(s) 214, 307
LweI GCATC 2 cut(s) 80, 251
MaeI CTAG 3 cut(s) 153, 209, 435
MaeIII GTNAC 2 cut(s) 275, 451
MboII GAAGA 3 cut(s) 141, 405, 408
MlyI GAGTC 1 cut(s) 74
MmeI TCCRAC 1 cut(s) 311
MnlI CCTC 5 cut(s) 157, 277, 294, 411, 414
MseI TTAA 1 cut(s) 9
MspA1I CMGCKG 1 cut(s) 227
MspI CCGG 1 cut(s) 389
MspR9I CCNGG 3 cut(s) 237, 389, 390
MvaI CCWGG 1 cut(s) 237
MwoI GCNNNNNNNGC 8 cut(s) 41, 180, 189, 198, 262, 326, 431, 464
NciI CCSGG 2 cut(s) 389, 390
NheI GCTAGC 1 cut(s) 208
NlaIII CATG 1 cut(s) 358
NmuCI GTSAC 1 cut(s) 451
NsbI TGCGCA 1 cut(s) 328
PaqCI CACCTGC 1 cut(s) 430
PfeI GAWTC 1 cut(s) 410
PkrI GCNGC 2 cut(s) 229, 322
PleI GAGTC 1 cut(s) 74
PpsI GAGTC 1 cut(s) 74
Psp6I CCWGG 1 cut(s) 235
PspFI CCCAGC 1 cut(s) 127
PspGI CCWGG 1 cut(s) 235
PspPI GGNCC 2 cut(s) 125, 199
PstNI CAGNNNCTG 1 cut(s) 338
PvuII CAGCTG 1 cut(s) 227
RsaI GTAC 3 cut(s) 54, 63, 369
RsaNI GTAC 3 cut(s) 53, 62, 368
SaqAI TTAA 1 cut(s) 9
SatI GCNGC 2 cut(s) 228, 321
Sau96I GGNCC 2 cut(s) 125, 199
SchI GAGTC 1 cut(s) 74
ScrFI CCNGG 3 cut(s) 237, 389, 390
SetI ASST 7 cut(s) 115, 149, 210, 229, 250, 424, 446
SfaNI GCATC 2 cut(s) 80, 251
SmaI CCCGGG 1 cut(s) 390
SsiI CCGC 1 cut(s) 263
SspI AATATT 1 cut(s) 14
SspMI CTAG 3 cut(s) 153, 209, 435
StyD4I CCNGG 3 cut(s) 235, 387, 388
TaaI ACNGT 2 cut(s) 220, 376
TaqI TCGA 1 cut(s) 78
TaqII GACCGA 1 cut(s) 439
TatI WGTACW 1 cut(s) 52
TfiI GAWTC 1 cut(s) 410
Tru1I TTAA 1 cut(s) 9
Tru9I TTAA 1 cut(s) 9
TscAI CASTG 1 cut(s) 379
TseFI GTSAC 1 cut(s) 451
TseI GCWGC 2 cut(s) 227, 320
Tsp45I GTSAC 1 cut(s) 451
TspDTI ATGAA 1 cut(s) 371
TspMI CCCGGG 1 cut(s) 388
TspRI CASTG 1 cut(s) 379
XmaI CCCGGG 1 cut(s) 388
XspI CTAG 3 cut(s) 153, 209, 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.