Rroxscaffold_2G00109150

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001282
Physical Location & Seq
Forward (+)
33267572 .. 33268702
1131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00109150.1

Sequence Viewer

Length: 573 bp
ATGGAGAGCCAGAAGAAGATAATGGTTGTTTTTGGGTTAGTTGCGGCTGTGTTTTTACAGTGTGTGGCGGCACAGACGACCCATGTGGTGGGAGGTAGCTTAGGCTGGAACATACCACCGACCGGTGCACAAGAGTATGTCACATGGGCGTCTGGTCAGAAGTTCGTAGTTGGTGATTTTCTGACTTTCAACTTCGCGACAGGCACACACGACGTGGTGGAAGTACCCAAAGCATCTTTCGACTCTTGCAGCTCCACCAACCAAATCGGCAGCATCATCAACACCGGCCCGGCCAACATCACTCTCACCCCCGCCGGCGAACACTACTACATCTGTACTTTCGGCAACCACTGCCAGTCCGGCCAGAAGCTCGCCATCACCGTCTTAGCATCCGCCACGTCACCCGGAGGCTCTCCTTCTGCACCACCTCCATCTCCTTCTGCACCATCTCCATCTACCACAGCAAACAACACGCCGGGGGTGACCACGGTGCCGCCGAGCTCTTCATCCCCTGCTGCTGGGGCTGGTTTCGTTTTGTGCTCGTTGTCCCTTGTAATGGGATTGTTCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

190

Amino Acids

19.34

Weight (kDa)

6.0

Isoelectric Point (pI)

53.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 41 - 122 1.1e-23 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 490
AccB7I CCANNNNNTGG 1 cut(s) 88
AccII CGCG 1 cut(s) 197
AciI CCGC 5 cut(s) 44, 68, 312, 393, 494
AcoI YGGCCR 2 cut(s) 291, 361
AcyI GRCGYC 1 cut(s) 149
AdeI CACNNNGTG 1 cut(s) 214
AfaI GTAC 2 cut(s) 225, 337
AfiI CCNNNNNNNGG 4 cut(s) 88, 122, 518, 556
AgeI ACCGGT 1 cut(s) 122
AgsI TTSAA 1 cut(s) 190
AjiI CACGTC 2 cut(s) 214, 399
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AluBI AGCT 4 cut(s) 99, 252, 370, 501
AluI AGCT 4 cut(s) 99, 252, 370, 501
Alw21I GWGCWC 3 cut(s) 130, 503, 542
Alw44I GTGCAC 1 cut(s) 126
AoxI GGCC 3 cut(s) 286, 291, 361
ApaLI GTGCAC 1 cut(s) 126
ApeKI GCWGC 3 cut(s) 249, 270, 515
AsiGI ACCGGT 1 cut(s) 122
AspS9I GGNCC 1 cut(s) 287
AsuC2I CCSGG 3 cut(s) 290, 405, 477
AsuHPI GGTGA 5 cut(s) 185, 298, 370, 393, 493
BaeGI GKGCMC 1 cut(s) 130
BanI GGYRCC 1 cut(s) 490
BanII GRGCYC 1 cut(s) 503
Bbv12I GWGCWC 3 cut(s) 130, 503, 542
BbvI GCAGC 3 cut(s) 261, 282, 502
BccI CCATC 4 cut(s) 383, 439, 454, 460
BcnI CCSGG 3 cut(s) 290, 405, 477
BglI GCCNNNNNGGC 1 cut(s) 360
BisI GCNGC 6 cut(s) 45, 69, 250, 271, 494, 516
BlsI GCNGC 6 cut(s) 46, 70, 251, 272, 495, 517
Bme1390I CCNGG 3 cut(s) 290, 405, 477
BmgBI CACGTC 2 cut(s) 214, 399
BmgT120I GGNCC 1 cut(s) 287
BmiI GGNNCC 1 cut(s) 492
BmrFI CCNGG 3 cut(s) 290, 405, 477
BmsI GCATC 3 cut(s) 242, 282, 398
Bpu10I CCTNAGC 1 cut(s) 100
BpuMI CCSGG 3 cut(s) 290, 405, 477
BsaHI GRCGYC 1 cut(s) 149
BsaJI CCNNGG 2 cut(s) 476, 486
BsaWI WCCGGW 1 cut(s) 122
Bsc4I CCNNNNNNNGG 4 cut(s) 88, 122, 518, 556
Bse118I RCCGGY 3 cut(s) 122, 284, 314
Bse1I ACTGG 1 cut(s) 355
BseDI CCNNGG 2 cut(s) 476, 486
BseGI GGATG 2 cut(s) 389, 506
BseLI CCNNNNNNNGG 4 cut(s) 88, 122, 518, 556
BseNI ACTGG 1 cut(s) 355
BseSI GKGCMC 1 cut(s) 130
BseXI GCAGC 3 cut(s) 261, 282, 502
BseYI CCCAGC 1 cut(s) 518
BsgI GTGCAG 2 cut(s) 405, 426
Bsh1236I CGCG 1 cut(s) 197
Bsh1285I CGRYCG 1 cut(s) 123
BshFI GGCC 3 cut(s) 288, 293, 363
BshNI GGYRCC 1 cut(s) 490
BshTI ACCGGT 1 cut(s) 122
BsiEI CGRYCG 1 cut(s) 123
BsiHKAI GWGCWC 3 cut(s) 130, 503, 542
BsiSI CCGG 7 cut(s) 123, 285, 290, 315, 360, 405, 476
BslFI GGGAC 1 cut(s) 532
BslI CCNNNNNNNGG 4 cut(s) 88, 122, 518, 556
BsmFI GGGAC 1 cut(s) 532
BsnI GGCC 3 cut(s) 288, 293, 363
Bsp1286I GDGCHC 3 cut(s) 130, 503, 542
Bsp68I TCGCGA 1 cut(s) 197
BspACI CCGC 5 cut(s) 44, 68, 312, 393, 494
BspANI GGCC 3 cut(s) 288, 293, 363
BspFNI CGCG 1 cut(s) 197
BspLI GGNNCC 1 cut(s) 492
BspQI GCTCTTC 1 cut(s) 508
BspT107I GGYRCC 1 cut(s) 490
BsrFI RCCGGY 3 cut(s) 122, 284, 314
BsrI ACTGG 1 cut(s) 355
BssAI RCCGGY 3 cut(s) 122, 284, 314
BssECI CCNNGG 2 cut(s) 476, 486
BssNI GRCGYC 1 cut(s) 149
Bst4CI ACNGT 3 cut(s) 60, 382, 490
Bst6I CTCTTC 1 cut(s) 508
BstACI GRCGYC 1 cut(s) 149
BstAPI GCANNNNNTGC 1 cut(s) 351
BstC8I GCNNGC 2 cut(s) 316, 372
BstDEI CTNAG 2 cut(s) 100, 385
BstDSI CCRYGG 1 cut(s) 486
BstEII GGTNACC 1 cut(s) 481
BstF5I GGATG 2 cut(s) 389, 506
BstFNI CGCG 1 cut(s) 197
BstMCI CGRYCG 1 cut(s) 123
BstMWI GCNNNNNNNGC 3 cut(s) 351, 360, 521
BstPI GGTNACC 1 cut(s) 481
BstSCI CCNGG 3 cut(s) 288, 403, 475
BstSLI GKGCMC 1 cut(s) 130
BstUI CGCG 1 cut(s) 197
BstV1I GCAGC 3 cut(s) 261, 282, 502
BsuRI GGCC 3 cut(s) 288, 293, 363
BtgI CCRYGG 1 cut(s) 486
BtrI CACGTC 2 cut(s) 214, 399
BtsCI GGATG 2 cut(s) 389, 506
BtsI GCAGTG 1 cut(s) 349
BtsIMutI CAGTG 2 cut(s) 65, 349
BtuMI TCGCGA 1 cut(s) 197
Cac8I GCNNGC 2 cut(s) 316, 372
Cfr10I RCCGGY 3 cut(s) 122, 284, 314
Cfr13I GGNCC 1 cut(s) 287
CseI GACGC 1 cut(s) 138
Csp6I GTAC 2 cut(s) 224, 336
CspAI ACCGGT 1 cut(s) 122
CviAII CATG 2 cut(s) 83, 144
CviQI GTAC 2 cut(s) 224, 336
DdeI CTNAG 2 cut(s) 100, 385
DraIII CACNNNGTG 1 cut(s) 214
EaeI YGGCCR 2 cut(s) 291, 361
Eam1104I CTCTTC 1 cut(s) 508
EarI CTCTTC 1 cut(s) 508
EciI GGCGGA 1 cut(s) 382
Ecl136II GAGCTC 1 cut(s) 501
Eco24I GRGCYC 1 cut(s) 503
Eco53kI GAGCTC 1 cut(s) 501
Eco91I GGTNACC 1 cut(s) 481
EcoICRI GAGCTC 1 cut(s) 501
EcoO65I GGTNACC 1 cut(s) 481
EcoT38I GRGCYC 1 cut(s) 503
FaeI CATG 2 cut(s) 86, 147
FaiI YATR 4 cut(s) 84, 113, 138, 145
FaqI GGGAC 1 cut(s) 532
FatI CATG 2 cut(s) 82, 143
FauI CCCGC 1 cut(s) 319
Fnu4HI GCNGC 6 cut(s) 45, 69, 250, 271, 494, 516
FokI GGATG 2 cut(s) 376, 493
FriOI GRGCYC 1 cut(s) 503
Fsp4HI GCNGC 6 cut(s) 45, 69, 250, 271, 494, 516
GluI GCNGC 6 cut(s) 45, 69, 250, 271, 494, 516
GsaI CCCAGC 1 cut(s) 522
HaeIII GGCC 3 cut(s) 288, 293, 363
HapII CCGG 7 cut(s) 123, 285, 290, 315, 360, 405, 476
HgaI GACGC 1 cut(s) 138
Hin1I GRCGYC 1 cut(s) 149
Hin1II CATG 2 cut(s) 86, 147
HinfI GANTC 1 cut(s) 242
HpaII CCGG 7 cut(s) 123, 285, 290, 315, 360, 405, 476
HphI GGTGA 5 cut(s) 185, 298, 370, 393, 493
Hpy166II GTNNAC 1 cut(s) 128
Hpy188I TCNGA 2 cut(s) 159, 183
Hpy188III TCNNGA 1 cut(s) 196
Hpy8I GTNNAC 1 cut(s) 128
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 2 cut(s) 426, 447
HpyCH4III ACNGT 3 cut(s) 60, 382, 490
HpyCH4IV ACGT 2 cut(s) 213, 398
HpyCH4V TGCA 4 cut(s) 128, 249, 422, 443
HpyF10VI GCNNNNNNNGC 3 cut(s) 351, 360, 521
HpyF3I CTNAG 2 cut(s) 100, 385
HpySE526I ACGT 2 cut(s) 213, 398
Hsp92I GRCGYC 1 cut(s) 149
Hsp92II CATG 2 cut(s) 86, 147
KroI GCCGGC 1 cut(s) 314
KroNI GCCGGC 1 cut(s) 316
LguI GCTCTTC 1 cut(s) 508
LmnI GCTCC 1 cut(s) 257
Lsp1109I GCAGC 3 cut(s) 261, 282, 502
LweI GCATC 3 cut(s) 242, 282, 398
MaeII ACGT 2 cut(s) 213, 398
MaeIII GTNAC 3 cut(s) 139, 399, 481
MboII GAAGA 3 cut(s) 25, 28, 495
MhlI GDGCHC 3 cut(s) 130, 503, 542
MlyI GAGTC 1 cut(s) 236
MnlI CCTC 3 cut(s) 86, 401, 438
MreI CGCCGGCG 1 cut(s) 314
MroNI GCCGGC 1 cut(s) 314
MspI CCGG 7 cut(s) 123, 285, 290, 315, 360, 405, 476
MspR9I CCNGG 3 cut(s) 290, 405, 477
MvnI CGCG 1 cut(s) 197
MwoI GCNNNNNNNGC 3 cut(s) 351, 360, 521
NaeI GCCGGC 1 cut(s) 316
NciI CCSGG 3 cut(s) 290, 405, 477
NgoMIV GCCGGC 1 cut(s) 314
NlaIII CATG 2 cut(s) 86, 147
NlaIV GGNNCC 1 cut(s) 492
NmeAIII GCCGAG 1 cut(s) 522
NmuCI GTSAC 3 cut(s) 139, 399, 481
NruI TCGCGA 1 cut(s) 197
PciSI GCTCTTC 1 cut(s) 508
PcsI WCGNNNNNNNCGW 2 cut(s) 378, 494
PdiI GCCGGC 1 cut(s) 316
PflMI CCANNNNNTGG 1 cut(s) 88
PinAI ACCGGT 1 cut(s) 122
PkrI GCNGC 6 cut(s) 46, 70, 251, 272, 495, 517
PleI GAGTC 1 cut(s) 236
PpsI GAGTC 1 cut(s) 236
Psp124BI GAGCTC 1 cut(s) 503
PspEI GGTNACC 1 cut(s) 481
PspFI CCCAGC 1 cut(s) 518
PspN4I GGNNCC 1 cut(s) 492
PspPI GGNCC 1 cut(s) 287
RruI TCGCGA 1 cut(s) 197
RsaI GTAC 2 cut(s) 225, 337
RsaNI GTAC 2 cut(s) 224, 336
SacI GAGCTC 1 cut(s) 503
SapI GCTCTTC 1 cut(s) 508
SatI GCNGC 6 cut(s) 45, 69, 250, 271, 494, 516
Sau96I GGNCC 1 cut(s) 287
SchI GAGTC 1 cut(s) 236
ScrFI CCNGG 3 cut(s) 290, 405, 477
SduI GDGCHC 3 cut(s) 130, 503, 542
SetI ASST 8 cut(s) 97, 101, 216, 254, 372, 401, 430, 503
SfaNI GCATC 3 cut(s) 242, 282, 398
SgrAI CRCCGGYG 1 cut(s) 314
SsiI CCGC 5 cut(s) 44, 68, 312, 393, 494
SstI GAGCTC 1 cut(s) 503
StyD4I CCNGG 3 cut(s) 288, 403, 475
TaaI ACNGT 3 cut(s) 60, 382, 490
TaiI ACGT 2 cut(s) 216, 401
TaqI TCGA 1 cut(s) 240
TatI WGTACW 1 cut(s) 335
TauI GCSGC 3 cut(s) 47, 71, 496
TscAI CASTG 2 cut(s) 65, 356
TseFI GTSAC 3 cut(s) 139, 399, 481
TseI GCWGC 3 cut(s) 249, 270, 515
Tsp45I GTSAC 3 cut(s) 139, 399, 481
TspDTI ATGAA 1 cut(s) 495
TspRI CASTG 2 cut(s) 65, 356
Van91I CCANNNNNTGG 1 cut(s) 88
VneI GTGCAC 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.