RchiOBHm_Chr2g0135581

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001282
Physical Location & Seq
Reverse (-)
52690258 .. 52691462
1205 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50645

Sequence Viewer

Length: 552 bp
ATGGAGAGCCAGAAGAAGATGATGGTTGTTTTTGGGTTAGTTGCGGCTGTGTTTTTACAGTGTGTGGCGGCACAGACGACCCATGTGGTGGGAGATAGCGTAGGCTGGATCATACCACCGAACGGTGCACAACAGTATGTCACATGGGCGTCTGGTCAGAAGTTCGTAGTTGGTGATTTTCTGATTTTCAACTTCACGACAAACTCACACGACGTTCTGGAAGTACCCAAAGCATCGTTCGACTCTTGCAGCTCCACCAACCCAATTGGCAACATCATCACCACCGGCCCGGCCAACATCACTCTCACCTCCTCCGGCGAACACTACTACATCTGCACTTTCGGCCGCCACTGCCAGTCCGGCCAGAAGCTCGCCATCTCCGTCTCAGCATCCGCCACGTCACCCGGATCCTCCCCTTCTGCACCATCTCCATCTACCACAGCTGGCACCACGCCGGGGGTGACCGCGGTGCCGCCAAGCTCTTCATCCCCTGCTGTTGGGGCTGGTTTCGTTTTGTGCTCGTTGTCCCTTGTAATGGGATTGTTCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

183

Amino Acids

18.83

Weight (kDa)

6.37

Isoelectric Point (pI)

49.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 41 - 122 7.4e-25 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 446, 469
AccB7I CCANNNNNTGG 1 cut(s) 88
AccII CGCG 1 cut(s) 467
AciI CCGC 7 cut(s) 44, 68, 346, 393, 465, 467, 473
AclWI GGATC 3 cut(s) 116, 402, 415
AcoI YGGCCR 3 cut(s) 291, 343, 361
AcyI GRCGYC 1 cut(s) 149
AfaI GTAC 1 cut(s) 225
AfiI CCNNNNNNNGG 5 cut(s) 88, 122, 456, 497, 535
AgsI TTSAA 1 cut(s) 190
AjiI CACGTC 1 cut(s) 399
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AluBI AGCT 4 cut(s) 252, 370, 443, 480
AluI AGCT 4 cut(s) 252, 370, 443, 480
Alw21I GWGCWC 2 cut(s) 130, 521
Alw26I GTCTC 1 cut(s) 388
Alw44I GTGCAC 1 cut(s) 126
AlwI GGATC 3 cut(s) 116, 402, 415
AoxI GGCC 4 cut(s) 286, 291, 343, 361
ApaLI GTGCAC 1 cut(s) 126
ApeKI GCWGC 1 cut(s) 249
AspS9I GGNCC 1 cut(s) 287
AsuC2I CCSGG 3 cut(s) 290, 405, 456
AsuHPI GGTGA 5 cut(s) 185, 271, 298, 393, 472
BaeGI GKGCMC 1 cut(s) 130
BamHI GGATCC 1 cut(s) 407
BanI GGYRCC 2 cut(s) 446, 469
Bbv12I GWGCWC 2 cut(s) 130, 521
BbvI GCAGC 1 cut(s) 261
BccI CCATC 4 cut(s) 16, 383, 433, 439
BcnI CCSGG 3 cut(s) 290, 405, 456
BcoDI GTCTC 1 cut(s) 388
BglI GCCNNNNNGGC 1 cut(s) 360
BisI GCNGC 5 cut(s) 45, 69, 250, 346, 473
BlsI GCNGC 5 cut(s) 46, 70, 251, 347, 474
Bme1390I CCNGG 3 cut(s) 290, 405, 456
BmgBI CACGTC 1 cut(s) 399
BmgT120I GGNCC 1 cut(s) 287
BmiI GGNNCC 3 cut(s) 409, 448, 471
BmrFI CCNGG 3 cut(s) 290, 405, 456
BmsI GCATC 2 cut(s) 242, 398
BpuMI CCSGG 3 cut(s) 290, 405, 456
BsaHI GRCGYC 1 cut(s) 149
BsaJI CCNNGG 2 cut(s) 455, 465
BsaXI ACNNNNNCTCC 2 cut(s) 84, 114
Bsc4I CCNNNNNNNGG 5 cut(s) 88, 122, 456, 497, 535
Bse118I RCCGGY 1 cut(s) 284
Bse1I ACTGG 1 cut(s) 355
BseDI CCNNGG 2 cut(s) 455, 465
BseGI GGATG 2 cut(s) 389, 485
BseLI CCNNNNNNNGG 5 cut(s) 88, 122, 456, 497, 535
BseMII CTCAG 1 cut(s) 399
BseNI ACTGG 1 cut(s) 355
BseRI GAGGAG 1 cut(s) 301
BseSI GKGCMC 1 cut(s) 130
BseX3I CGGCCG 1 cut(s) 343
BseXI GCAGC 1 cut(s) 261
BsgI GTGCAG 2 cut(s) 319, 405
Bsh1236I CGCG 1 cut(s) 467
Bsh1285I CGRYCG 1 cut(s) 346
BshFI GGCC 4 cut(s) 288, 293, 345, 363
BshNI GGYRCC 2 cut(s) 446, 469
BsiEI CGRYCG 1 cut(s) 346
BsiHKAI GWGCWC 2 cut(s) 130, 521
BsiSI CCGG 6 cut(s) 285, 290, 315, 360, 405, 455
BslFI GGGAC 1 cut(s) 511
BslI CCNNNNNNNGG 5 cut(s) 88, 122, 456, 497, 535
BsmAI GTCTC 1 cut(s) 388
BsmBI CGTCTC 1 cut(s) 388
BsmFI GGGAC 1 cut(s) 511
BsnI GGCC 4 cut(s) 288, 293, 345, 363
Bsp1286I GDGCHC 2 cut(s) 130, 521
Bsp143I GATC 2 cut(s) 108, 407
BspACI CCGC 7 cut(s) 44, 68, 346, 393, 465, 467, 473
BspANI GGCC 4 cut(s) 288, 293, 345, 363
BspCNI CTCAG 1 cut(s) 398
BspFNI CGCG 1 cut(s) 467
BspLI GGNNCC 3 cut(s) 409, 448, 471
BspPI GGATC 3 cut(s) 116, 402, 415
BspQI GCTCTTC 1 cut(s) 487
BspT107I GGYRCC 2 cut(s) 446, 469
BsrFI RCCGGY 1 cut(s) 284
BsrI ACTGG 1 cut(s) 355
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 2 cut(s) 455, 465
BssMI GATC 2 cut(s) 108, 407
BssNI GRCGYC 1 cut(s) 149
Bst4CI ACNGT 3 cut(s) 60, 125, 135
Bst6I CTCTTC 1 cut(s) 487
BstACI GRCGYC 1 cut(s) 149
BstC8I GCNNGC 2 cut(s) 372, 445
BstDEI CTNAG 1 cut(s) 385
BstDSI CCRYGG 1 cut(s) 465
BstEII GGTNACC 1 cut(s) 460
BstF5I GGATG 2 cut(s) 389, 485
BstFNI CGCG 1 cut(s) 467
BstKTI GATC 2 cut(s) 111, 410
BstMAI GTCTC 1 cut(s) 388
BstMBI GATC 2 cut(s) 108, 407
BstMCI CGRYCG 1 cut(s) 346
BstMWI GCNNNNNNNGC 4 cut(s) 342, 351, 360, 500
BstPI GGTNACC 1 cut(s) 460
BstSCI CCNGG 3 cut(s) 288, 403, 454
BstSLI GKGCMC 1 cut(s) 130
BstUI CGCG 1 cut(s) 467
BstV1I GCAGC 1 cut(s) 261
BstX2I RGATCY 1 cut(s) 407
BstYI RGATCY 1 cut(s) 407
BstZI CGGCCG 1 cut(s) 343
BsuRI GGCC 4 cut(s) 288, 293, 345, 363
BtgI CCRYGG 1 cut(s) 465
BtrI CACGTC 1 cut(s) 399
BtsCI GGATG 2 cut(s) 389, 485
BtsI GCAGTG 1 cut(s) 349
BtsIMutI CAGTG 2 cut(s) 65, 349
Cac8I GCNNGC 2 cut(s) 372, 445
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 1 cut(s) 287
Cfr42I CCGCGG 1 cut(s) 468
CseI GACGC 1 cut(s) 138
Csp6I GTAC 1 cut(s) 224
CviAII CATG 2 cut(s) 83, 144
CviQI GTAC 1 cut(s) 224
DdeI CTNAG 1 cut(s) 385
DpnI GATC 2 cut(s) 110, 409
DpnII GATC 2 cut(s) 108, 407
EaeI YGGCCR 3 cut(s) 291, 343, 361
EagI CGGCCG 1 cut(s) 343
Eam1104I CTCTTC 1 cut(s) 487
EarI CTCTTC 1 cut(s) 487
EciI GGCGGA 1 cut(s) 382
EclXI CGGCCG 1 cut(s) 343
Eco52I CGGCCG 1 cut(s) 343
Eco91I GGTNACC 1 cut(s) 460
EcoO65I GGTNACC 1 cut(s) 460
Esp3I CGTCTC 1 cut(s) 388
FaeI CATG 2 cut(s) 86, 147
FaiI YATR 4 cut(s) 84, 113, 138, 145
FaqI GGGAC 1 cut(s) 511
FatI CATG 2 cut(s) 82, 143
Fnu4HI GCNGC 5 cut(s) 45, 69, 250, 346, 473
FokI GGATG 2 cut(s) 376, 472
Fsp4HI GCNGC 5 cut(s) 45, 69, 250, 346, 473
GluI GCNGC 5 cut(s) 45, 69, 250, 346, 473
HaeIII GGCC 4 cut(s) 288, 293, 345, 363
HapII CCGG 6 cut(s) 285, 290, 315, 360, 405, 455
HgaI GACGC 1 cut(s) 138
Hin1I GRCGYC 1 cut(s) 149
Hin1II CATG 2 cut(s) 86, 147
HinfI GANTC 1 cut(s) 242
HpaII CCGG 6 cut(s) 285, 290, 315, 360, 405, 455
HphI GGTGA 5 cut(s) 185, 271, 298, 393, 472
Hpy166II GTNNAC 1 cut(s) 128
Hpy188I TCNGA 2 cut(s) 159, 183
Hpy188III TCNNGA 2 cut(s) 196, 218
Hpy8I GTNNAC 1 cut(s) 128
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 1 cut(s) 426
HpyCH4III ACNGT 3 cut(s) 60, 125, 135
HpyCH4IV ACGT 2 cut(s) 213, 398
HpyCH4V TGCA 4 cut(s) 128, 249, 336, 422
HpyF10VI GCNNNNNNNGC 4 cut(s) 342, 351, 360, 500
HpyF3I CTNAG 1 cut(s) 385
HpySE526I ACGT 2 cut(s) 213, 398
Hsp92I GRCGYC 1 cut(s) 149
Hsp92II CATG 2 cut(s) 86, 147
KspI CCGCGG 1 cut(s) 468
Kzo9I GATC 2 cut(s) 108, 407
LguI GCTCTTC 1 cut(s) 487
LmnI GCTCC 1 cut(s) 257
Lsp1109I GCAGC 1 cut(s) 261
LweI GCATC 2 cut(s) 242, 398
MaeII ACGT 2 cut(s) 213, 398
MaeIII GTNAC 3 cut(s) 139, 399, 460
MalI GATC 2 cut(s) 110, 409
MboI GATC 2 cut(s) 108, 407
MboII GAAGA 3 cut(s) 25, 28, 474
MfeI CAATTG 1 cut(s) 264
MflI RGATCY 1 cut(s) 407
MhlI GDGCHC 2 cut(s) 130, 521
MluCI AATT 1 cut(s) 264
MlyI GAGTC 1 cut(s) 236
MnlI CCTC 3 cut(s) 319, 322, 421
MspA1I CMGCKG 2 cut(s) 443, 467
MspI CCGG 6 cut(s) 285, 290, 315, 360, 405, 455
MspR9I CCNGG 3 cut(s) 290, 405, 456
MunI CAATTG 1 cut(s) 264
MvnI CGCG 1 cut(s) 467
MwoI GCNNNNNNNGC 4 cut(s) 342, 351, 360, 500
NciI CCSGG 3 cut(s) 290, 405, 456
NdeII GATC 2 cut(s) 108, 407
NlaIII CATG 2 cut(s) 86, 147
NlaIV GGNNCC 3 cut(s) 409, 448, 471
NmuCI GTSAC 3 cut(s) 139, 399, 460
PciSI GCTCTTC 1 cut(s) 487
PcsI WCGNNNNNNNCGW 1 cut(s) 378
PflMI CCANNNNNTGG 1 cut(s) 88
PkrI GCNGC 5 cut(s) 46, 70, 251, 347, 474
PleI GAGTC 1 cut(s) 236
PpsI GAGTC 1 cut(s) 236
PspEI GGTNACC 1 cut(s) 460
PspN4I GGNNCC 3 cut(s) 409, 448, 471
PspPI GGNCC 1 cut(s) 287
PsuI RGATCY 1 cut(s) 407
PvuII CAGCTG 1 cut(s) 443
RsaI GTAC 1 cut(s) 225
RsaNI GTAC 1 cut(s) 224
SacII CCGCGG 1 cut(s) 468
SapI GCTCTTC 1 cut(s) 487
SatI GCNGC 5 cut(s) 45, 69, 250, 346, 473
Sau3AI GATC 2 cut(s) 108, 407
Sau96I GGNCC 1 cut(s) 287
SchI GAGTC 1 cut(s) 236
ScrFI CCNGG 3 cut(s) 290, 405, 456
SduI GDGCHC 2 cut(s) 130, 521
SetI ASST 7 cut(s) 216, 254, 311, 372, 401, 445, 482
SfaNI GCATC 2 cut(s) 242, 398
Sfr303I CCGCGG 1 cut(s) 468
SgrBI CCGCGG 1 cut(s) 468
Sse9I AATT 1 cut(s) 264
SsiI CCGC 7 cut(s) 44, 68, 346, 393, 465, 467, 473
StyD4I CCNGG 3 cut(s) 288, 403, 454
TaaI ACNGT 3 cut(s) 60, 125, 135
TaiI ACGT 2 cut(s) 216, 401
TaqI TCGA 1 cut(s) 240
TasI AATT 1 cut(s) 264
TauI GCSGC 4 cut(s) 47, 71, 348, 475
TscAI CASTG 2 cut(s) 65, 356
TseFI GTSAC 3 cut(s) 139, 399, 460
TseI GCWGC 1 cut(s) 249
Tsp45I GTSAC 3 cut(s) 139, 399, 460
TspDTI ATGAA 1 cut(s) 474
TspGWI ACGGA 1 cut(s) 370
TspRI CASTG 2 cut(s) 65, 356
Van91I CCANNNNNTGG 1 cut(s) 88
VneI GTGCAC 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.