Rroxscaffold_2G00109140

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001282
Physical Location & Seq
Forward (+)
33236387 .. 33237887
1501 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00109140.1

Sequence Viewer

Length: 660 bp
ATGGAGAGCCAGAAGAAGATGATGGTTGTTTTTGGGTTTCTTGCGGCTGTGTTTTTGGAGAGTGTGGCGGCGCAAACGGCCCATGTGGTGGGAGGTAGCGTAGGCTGGACCATACCACAGAATGGTGCACAAGAGTATGTCACTTGGGCGTCTGGTCAGAAGTTCATAGTTGGTGATGTTCTAATCTTCAACTTCGCGACAGACACACATGACGTAGTGCAAGTACCCAAAGCATCGTTCGACTCATGCAGCTCCGCCAACCCAATCGGCAGTACCATCACCACGGGCCCGGCCAACATCACTCTCACAGCTACCGGCGACCACTACTACATCTGCACTTTCGGCCGCCACTGCCAGTCCGGCCAGAAGCTCGCCATCACCGTCTCAGCATCCGCCATGTCACCCGGAGCCTCCCCTTCTGCACCAACTCCTATGTCTCCACCTCCACCCACTACCACTACTACTCCTACCACGCCTTCACCCACATCTGACGCCCCATGCCGTCCGGTCCAAGCACCATCTCCGTCCACCAAAACCACGGGGCCCACAGCTAGCACCACACCAGGATCCACCGTGCTACCGCCGAGCTCTTCTTCCCCTGCCGTTGGGGCTGGTTTCGTTTTGTGCTTGCTGTCCCTTGTAATGGGATTGTTCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

219

Amino Acids

22.36

Weight (kDa)

6.38

Isoelectric Point (pI)

54.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 36 - 122 1.2e-25 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 88, 122
AccII CGCG 1 cut(s) 197
AciI CCGC 6 cut(s) 44, 68, 255, 346, 393, 581
AclWI GGATC 2 cut(s) 561, 574
AcoI YGGCCR 3 cut(s) 291, 343, 361
AcyI GRCGYC 2 cut(s) 149, 492
AfaI GTAC 2 cut(s) 225, 274
AfiI CCNNNNNNNGG 4 cut(s) 88, 122, 605, 643
AgsI TTSAA 1 cut(s) 190
AjnI CCWGG 1 cut(s) 562
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AluBI AGCT 5 cut(s) 252, 311, 370, 551, 588
AluI AGCT 5 cut(s) 252, 311, 370, 551, 588
Alw21I GWGCWC 2 cut(s) 130, 590
Alw26I GTCTC 2 cut(s) 388, 441
Alw44I GTGCAC 1 cut(s) 126
AlwI GGATC 2 cut(s) 561, 574
AoxI GGCC 6 cut(s) 78, 286, 291, 343, 361, 542
ApaI GGGCCC 2 cut(s) 290, 546
ApaLI GTGCAC 1 cut(s) 126
ApeKI GCWGC 1 cut(s) 249
AspLEI GCGC 1 cut(s) 73
AspS9I GGNCC 7 cut(s) 79, 108, 286, 287, 508, 542, 543
AsuC2I CCSGG 2 cut(s) 290, 405
AsuHPI GGTGA 5 cut(s) 185, 271, 370, 393, 471
AsuNHI GCTAGC 1 cut(s) 551
AvaII GGWCC 2 cut(s) 108, 508
BaeGI GKGCMC 3 cut(s) 130, 290, 546
BamHI GGATCC 1 cut(s) 566
BanII GRGCYC 3 cut(s) 290, 546, 590
Bbv12I GWGCWC 2 cut(s) 130, 590
BbvI GCAGC 1 cut(s) 261
BccI CCATC 4 cut(s) 16, 284, 383, 526
BceAI ACGGC 3 cut(s) 93, 486, 587
BciT130I CCWGG 1 cut(s) 564
BcnI CCSGG 2 cut(s) 290, 405
BcoDI GTCTC 2 cut(s) 388, 441
BfaI CTAG 1 cut(s) 552
BglI GCCNNNNNGGC 2 cut(s) 360, 608
BisI GCNGC 4 cut(s) 45, 69, 250, 346
BlsI GCNGC 4 cut(s) 46, 70, 251, 347
Bme1390I CCNGG 3 cut(s) 290, 405, 564
Bme18I GGWCC 2 cut(s) 108, 508
BmgT120I GGNCC 7 cut(s) 79, 108, 286, 287, 508, 542, 543
BmiI GGNNCC 5 cut(s) 288, 409, 543, 544, 568
BmrFI CCNGG 3 cut(s) 290, 405, 564
BmsI GCATC 2 cut(s) 242, 398
BmtI GCTAGC 1 cut(s) 555
BpuMI CCSGG 2 cut(s) 290, 405
BsaHI GRCGYC 2 cut(s) 149, 492
BsaJI CCNNGG 2 cut(s) 282, 537
BsaWI WCCGGW 1 cut(s) 505
BsaXI ACNNNNNCTCC 4 cut(s) 84, 114, 448, 478
Bsc4I CCNNNNNNNGG 4 cut(s) 88, 122, 605, 643
Bse118I RCCGGY 1 cut(s) 314
Bse1I ACTGG 1 cut(s) 355
BseBI CCWGG 1 cut(s) 564
BseDI CCNNGG 2 cut(s) 282, 537
BseGI GGATG 1 cut(s) 389
BseLI CCNNNNNNNGG 4 cut(s) 88, 122, 605, 643
BseMII CTCAG 1 cut(s) 399
BseNI ACTGG 1 cut(s) 355
BseSI GKGCMC 3 cut(s) 130, 290, 546
BseX3I CGGCCG 1 cut(s) 343
BseXI GCAGC 1 cut(s) 261
BsgI GTGCAG 2 cut(s) 319, 405
Bsh1236I CGCG 1 cut(s) 197
Bsh1285I CGRYCG 1 cut(s) 346
BshFI GGCC 6 cut(s) 80, 288, 293, 345, 363, 544
BsiEI CGRYCG 1 cut(s) 346
BsiHKAI GWGCWC 2 cut(s) 130, 590
BsiSI CCGG 5 cut(s) 290, 315, 360, 405, 506
BslFI GGGAC 1 cut(s) 619
BslI CCNNNNNNNGG 4 cut(s) 88, 122, 605, 643
BsmAI GTCTC 2 cut(s) 388, 441
BsmBI CGTCTC 1 cut(s) 388
BsmFI GGGAC 1 cut(s) 619
BsnI GGCC 6 cut(s) 80, 288, 293, 345, 363, 544
Bsp120I GGGCCC 2 cut(s) 286, 542
Bsp1286I GDGCHC 4 cut(s) 130, 290, 546, 590
Bsp143I GATC 1 cut(s) 566
Bsp68I TCGCGA 1 cut(s) 197
BspACI CCGC 6 cut(s) 44, 68, 255, 346, 393, 581
BspANI GGCC 6 cut(s) 80, 288, 293, 345, 363, 544
BspCNI CTCAG 1 cut(s) 398
BspFNI CGCG 1 cut(s) 197
BspLI GGNNCC 5 cut(s) 288, 409, 543, 544, 568
BspOI GCTAGC 1 cut(s) 555
BspPI GGATC 2 cut(s) 561, 574
BspQI GCTCTTC 1 cut(s) 595
BsrFI RCCGGY 1 cut(s) 314
BsrI ACTGG 1 cut(s) 355
BssAI RCCGGY 1 cut(s) 314
BssECI CCNNGG 2 cut(s) 282, 537
BssMI GATC 1 cut(s) 566
BssNI GRCGYC 2 cut(s) 149, 492
Bst2UI CCWGG 1 cut(s) 564
Bst4CI ACNGT 2 cut(s) 382, 574
Bst6I CTCTTC 1 cut(s) 595
BstACI GRCGYC 2 cut(s) 149, 492
BstC8I GCNNGC 3 cut(s) 372, 553, 629
BstDEI CTNAG 1 cut(s) 385
BstDSI CCRYGG 2 cut(s) 282, 537
BstF5I GGATG 1 cut(s) 389
BstFNI CGCG 1 cut(s) 197
BstHHI GCGC 1 cut(s) 73
BstKTI GATC 1 cut(s) 569
BstMAI GTCTC 2 cut(s) 388, 441
BstMBI GATC 1 cut(s) 566
BstMCI CGRYCG 1 cut(s) 346
BstMWI GCNNNNNNNGC 5 cut(s) 77, 342, 351, 360, 608
BstNI CCWGG 1 cut(s) 564
BstSCI CCNGG 3 cut(s) 288, 403, 562
BstSLI GKGCMC 3 cut(s) 130, 290, 546
BstUI CGCG 1 cut(s) 197
BstV1I GCAGC 1 cut(s) 261
BstX2I RGATCY 1 cut(s) 566
BstYI RGATCY 1 cut(s) 566
BstZI CGGCCG 1 cut(s) 343
BsuRI GGCC 6 cut(s) 80, 288, 293, 345, 363, 544
BtgI CCRYGG 2 cut(s) 282, 537
BtsCI GGATG 1 cut(s) 389
BtsI GCAGTG 1 cut(s) 349
BtsIMutI CAGTG 1 cut(s) 349
BtuMI TCGCGA 1 cut(s) 197
Cac8I GCNNGC 3 cut(s) 372, 553, 629
CfoI GCGC 1 cut(s) 73
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 7 cut(s) 79, 108, 286, 287, 508, 542, 543
CseI GACGC 2 cut(s) 138, 500
Csp6I GTAC 2 cut(s) 224, 273
CviAII CATG 5 cut(s) 83, 209, 246, 397, 498
CviQI GTAC 2 cut(s) 224, 273
DdeI CTNAG 1 cut(s) 385
DpnI GATC 1 cut(s) 568
DpnII GATC 1 cut(s) 566
EaeI YGGCCR 3 cut(s) 291, 343, 361
EagI CGGCCG 1 cut(s) 343
Eam1104I CTCTTC 1 cut(s) 595
EarI CTCTTC 1 cut(s) 595
EciI GGCGGA 2 cut(s) 244, 382
Ecl136II GAGCTC 1 cut(s) 588
EclXI CGGCCG 1 cut(s) 343
Eco24I GRGCYC 3 cut(s) 290, 546, 590
Eco47I GGWCC 2 cut(s) 108, 508
Eco52I CGGCCG 1 cut(s) 343
Eco53kI GAGCTC 1 cut(s) 588
EcoICRI GAGCTC 1 cut(s) 588
EcoO109I RGGNCCY 1 cut(s) 542
EcoRII CCWGG 1 cut(s) 562
EcoT38I GRGCYC 3 cut(s) 290, 546, 590
Esp3I CGTCTC 1 cut(s) 388
FaeI CATG 5 cut(s) 86, 212, 249, 400, 501
FaiI YATR 9 cut(s) 84, 113, 138, 167, 210, 247, 398, 434, 499
FaqI GGGAC 1 cut(s) 619
FatI CATG 5 cut(s) 82, 208, 245, 396, 497
Fnu4HI GCNGC 4 cut(s) 45, 69, 250, 346
FokI GGATG 1 cut(s) 376
FriOI GRGCYC 3 cut(s) 290, 546, 590
Fsp4HI GCNGC 4 cut(s) 45, 69, 250, 346
FspBI CTAG 1 cut(s) 552
GlaI GCGC 1 cut(s) 72
GluI GCNGC 4 cut(s) 45, 69, 250, 346
HaeIII GGCC 6 cut(s) 80, 288, 293, 345, 363, 544
HapII CCGG 5 cut(s) 290, 315, 360, 405, 506
HgaI GACGC 2 cut(s) 138, 500
HhaI GCGC 1 cut(s) 73
Hin1I GRCGYC 2 cut(s) 149, 492
Hin1II CATG 5 cut(s) 86, 212, 249, 400, 501
Hin6I GCGC 1 cut(s) 71
HinP1I GCGC 1 cut(s) 71
HinfI GANTC 1 cut(s) 242
HpaII CCGG 5 cut(s) 290, 315, 360, 405, 506
HphI GGTGA 5 cut(s) 185, 271, 370, 393, 471
Hpy166II GTNNAC 2 cut(s) 128, 528
Hpy188I TCNGA 2 cut(s) 159, 490
Hpy188III TCNNGA 1 cut(s) 196
Hpy8I GTNNAC 2 cut(s) 128, 528
HpyAV CCTTC 2 cut(s) 426, 486
HpyCH4III ACNGT 2 cut(s) 382, 574
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 5 cut(s) 128, 220, 249, 336, 422
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 342, 351, 360, 608
HpyF3I CTNAG 1 cut(s) 385
HpySE526I ACGT 1 cut(s) 213
Hsp92I GRCGYC 2 cut(s) 149, 492
Hsp92II CATG 5 cut(s) 86, 212, 249, 400, 501
HspAI GCGC 1 cut(s) 71
Kzo9I GATC 1 cut(s) 566
LguI GCTCTTC 1 cut(s) 595
LmnI GCTCC 2 cut(s) 257, 407
Lsp1109I GCAGC 1 cut(s) 261
LweI GCATC 2 cut(s) 242, 398
MaeI CTAG 1 cut(s) 552
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 2 cut(s) 139, 399
MalI GATC 1 cut(s) 568
MboI GATC 1 cut(s) 566
MboII GAAGA 5 cut(s) 25, 28, 178, 582, 585
MflI RGATCY 1 cut(s) 566
MhlI GDGCHC 4 cut(s) 130, 290, 546, 590
MlyI GAGTC 1 cut(s) 236
MnlI CCTC 3 cut(s) 86, 421, 453
MspI CCGG 5 cut(s) 290, 315, 360, 405, 506
MspR9I CCNGG 3 cut(s) 290, 405, 564
MvaI CCWGG 1 cut(s) 564
MvnI CGCG 1 cut(s) 197
MwoI GCNNNNNNNGC 5 cut(s) 77, 342, 351, 360, 608
NciI CCSGG 2 cut(s) 290, 405
NdeII GATC 1 cut(s) 566
NheI GCTAGC 1 cut(s) 551
NlaIII CATG 5 cut(s) 86, 212, 249, 400, 501
NlaIV GGNNCC 5 cut(s) 288, 409, 543, 544, 568
NmeAIII GCCGAG 1 cut(s) 609
NmuCI GTSAC 2 cut(s) 139, 399
NruI TCGCGA 1 cut(s) 197
PciSI GCTCTTC 1 cut(s) 595
PcsI WCGNNNNNNNCGW 1 cut(s) 378
PflMI CCANNNNNTGG 2 cut(s) 88, 122
PkrI GCNGC 4 cut(s) 46, 70, 251, 347
PleI GAGTC 1 cut(s) 236
PpsI GAGTC 1 cut(s) 236
Psp124BI GAGCTC 1 cut(s) 590
Psp6I CCWGG 1 cut(s) 562
PspGI CCWGG 1 cut(s) 562
PspN4I GGNNCC 5 cut(s) 288, 409, 543, 544, 568
PspOMI GGGCCC 2 cut(s) 286, 542
PspPI GGNCC 7 cut(s) 79, 108, 286, 287, 508, 542, 543
PsuI RGATCY 1 cut(s) 566
RruI TCGCGA 1 cut(s) 197
RsaI GTAC 2 cut(s) 225, 274
RsaNI GTAC 2 cut(s) 224, 273
SacI GAGCTC 1 cut(s) 590
SapI GCTCTTC 1 cut(s) 595
SatI GCNGC 4 cut(s) 45, 69, 250, 346
Sau3AI GATC 1 cut(s) 566
Sau96I GGNCC 7 cut(s) 79, 108, 286, 287, 508, 542, 543
SchI GAGTC 1 cut(s) 236
ScrFI CCNGG 3 cut(s) 290, 405, 564
SduI GDGCHC 4 cut(s) 130, 290, 546, 590
SetI ASST 8 cut(s) 97, 216, 254, 313, 372, 445, 553, 590
SfaNI GCATC 2 cut(s) 242, 398
SinI GGWCC 2 cut(s) 108, 508
SsiI CCGC 6 cut(s) 44, 68, 255, 346, 393, 581
SspMI CTAG 1 cut(s) 552
SstI GAGCTC 1 cut(s) 590
StyD4I CCNGG 3 cut(s) 288, 403, 562
TaaI ACNGT 2 cut(s) 382, 574
TaiI ACGT 1 cut(s) 216
TaqI TCGA 1 cut(s) 240
TauI GCSGC 3 cut(s) 47, 71, 348
TscAI CASTG 1 cut(s) 356
TseFI GTSAC 2 cut(s) 139, 399
TseI GCWGC 1 cut(s) 249
Tsp45I GTSAC 2 cut(s) 139, 399
TspDTI ATGAA 1 cut(s) 154
TspGWI ACGGA 1 cut(s) 513
TspRI CASTG 1 cut(s) 356
Van91I CCANNNNNTGG 2 cut(s) 88, 122
VneI GTGCAC 1 cut(s) 126
VpaK11BI GGWCC 2 cut(s) 108, 508
XspI CTAG 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.