Rroxscaffold_4G00301440

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001282
Physical Location & Seq
Forward (+)
21819361 .. 21820410
1050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00301440.1

Sequence Viewer

Length: 459 bp
ATGGAGAGCCAGAAGAAGATGATTGTTGTTTTTGGGTTAGTTGCTGCTGTGTTTTTACAGTGTGTGGCGGCGGAGACATCGCATGTGGTGGGAGATAGCTTAGGCTGGACCATACCACAGAACGGCGCACAAGAGTATGCCACTTGGGCGTCTGGTCAGAAGTTCGTAGTTGGTGATGTTCTGGTTTTCAGCTTCGTGACAGGCACACACGACGTGGTGGAAGTACCCAAAGCATCCTTCGATTCTTGCATCTCCACCAGCCCAATCGACAGCACCATCACCACCGGCCCGGCCAACATCACTCTTACCTCCTCCGGCGAACACTACTACATCTGTACTTTCGGCCTCCACTGCGAGTCCGGTCAGAAGCTCGCCATCACGGTCTCAGCATCCGCCACATCACCCGGAGACTCCCCTTCTGCACCATCTCTAACCACCGACCCTGACGACACGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

152

Amino Acids

15.9

Weight (kDa)

4.4

Isoelectric Point (pI)

44.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 36 - 122 6.2e-25 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 68, 71, 393
AcoI YGGCCR 1 cut(s) 291
AcyI GRCGYC 1 cut(s) 149
AdeI CACNNNGTG 1 cut(s) 214
AfaI GTAC 3 cut(s) 225, 337, 455
AfiI CCNNNNNNNGG 1 cut(s) 122
AflIII ACRYGT 1 cut(s) 450
AjiI CACGTC 1 cut(s) 214
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AluBI AGCT 3 cut(s) 99, 192, 370
AluI AGCT 3 cut(s) 99, 192, 370
Alw26I GTCTC 3 cut(s) 68, 388, 402
AoxI GGCC 3 cut(s) 286, 291, 343
ApeKI GCWGC 1 cut(s) 44
AspLEI GCGC 1 cut(s) 128
AspS9I GGNCC 2 cut(s) 108, 287
AsuC2I CCSGG 2 cut(s) 290, 405
AsuHPI GGTGA 3 cut(s) 185, 271, 393
AvaII GGWCC 1 cut(s) 108
BbvI GCAGC 1 cut(s) 31
BccI CCATC 3 cut(s) 284, 383, 433
BceAI ACGGC 1 cut(s) 139
BcnI CCSGG 2 cut(s) 290, 405
BcoDI GTCTC 3 cut(s) 68, 388, 402
BfaI CTAG 1 cut(s) 457
BglI GCCNNNNNGGC 1 cut(s) 146
BisI GCNGC 2 cut(s) 45, 69
BlsI GCNGC 2 cut(s) 46, 70
Bme1390I CCNGG 2 cut(s) 290, 405
Bme18I GGWCC 1 cut(s) 108
BmgBI CACGTC 1 cut(s) 214
BmgT120I GGNCC 2 cut(s) 108, 287
BmrFI CCNGG 2 cut(s) 290, 405
BmsI GCATC 3 cut(s) 242, 258, 398
Bpu10I CCTNAGC 1 cut(s) 100
BpuMI CCSGG 2 cut(s) 290, 405
BsaAI YACGTR 1 cut(s) 453
BsaHI GRCGYC 1 cut(s) 149
BsaI GGTCTC 1 cut(s) 388
BsaWI WCCGGW 1 cut(s) 359
Bsc4I CCNNNNNNNGG 1 cut(s) 122
Bse118I RCCGGY 1 cut(s) 284
BseGI GGATG 2 cut(s) 233, 389
BseLI CCNNNNNNNGG 1 cut(s) 122
BseMII CTCAG 1 cut(s) 399
BseRI GAGGAG 1 cut(s) 301
BseXI GCAGC 1 cut(s) 31
BsgI GTGCAG 1 cut(s) 405
BshFI GGCC 3 cut(s) 288, 293, 345
BsiSI CCGG 5 cut(s) 285, 290, 315, 360, 405
BslI CCNNNNNNNGG 1 cut(s) 122
BsmAI GTCTC 3 cut(s) 68, 388, 402
BsnI GGCC 3 cut(s) 288, 293, 345
Bso31I GGTCTC 1 cut(s) 388
BspACI CCGC 3 cut(s) 68, 71, 393
BspANI GGCC 3 cut(s) 288, 293, 345
BspCNI CTCAG 1 cut(s) 398
BspTNI GGTCTC 1 cut(s) 388
BsrFI RCCGGY 1 cut(s) 284
BssAI RCCGGY 1 cut(s) 284
BssNI GRCGYC 1 cut(s) 149
Bst4CI ACNGT 2 cut(s) 60, 382
BstACI GRCGYC 1 cut(s) 149
BstBAI YACGTR 1 cut(s) 453
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 2 cut(s) 100, 385
BstF5I GGATG 2 cut(s) 233, 389
BstHHI GCGC 1 cut(s) 128
BstMAI GTCTC 3 cut(s) 68, 388, 402
BstMWI GCNNNNNNNGC 2 cut(s) 146, 351
BstNSI RCATGY 1 cut(s) 86
BstSCI CCNGG 2 cut(s) 288, 403
BstV1I GCAGC 1 cut(s) 31
BsuRI GGCC 3 cut(s) 288, 293, 345
BtgZI GCGATG 1 cut(s) 63
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 2 cut(s) 233, 389
BtsI GCAGTG 1 cut(s) 349
BtsIMutI CAGTG 2 cut(s) 65, 349
Cac8I GCNNGC 1 cut(s) 372
CfoI GCGC 1 cut(s) 128
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 2 cut(s) 108, 287
CseI GACGC 1 cut(s) 138
Csp6I GTAC 3 cut(s) 224, 336, 454
CviAII CATG 1 cut(s) 83
CviJI RGCY 9 cut(s) 9, 99, 105, 192, 261, 288, 293, 345, 370
CviKI_1 RGCY 9 cut(s) 9, 99, 105, 192, 261, 288, 293, 345, 370
CviQI GTAC 3 cut(s) 224, 336, 454
DdeI CTNAG 2 cut(s) 100, 385
DraIII CACNNNGTG 1 cut(s) 214
EaeI YGGCCR 1 cut(s) 291
EciI GGCGGA 2 cut(s) 86, 382
Eco31I GGTCTC 1 cut(s) 388
Eco47I GGWCC 1 cut(s) 108
FaeI CATG 1 cut(s) 86
FaiI YATR 3 cut(s) 84, 113, 138
FatI CATG 1 cut(s) 82
Fnu4HI GCNGC 2 cut(s) 45, 69
FokI GGATG 2 cut(s) 220, 376
Fsp4HI GCNGC 2 cut(s) 45, 69
FspBI CTAG 1 cut(s) 457
GlaI GCGC 1 cut(s) 127
GluI GCNGC 2 cut(s) 45, 69
HaeIII GGCC 3 cut(s) 288, 293, 345
HapII CCGG 5 cut(s) 285, 290, 315, 360, 405
HgaI GACGC 1 cut(s) 138
HhaI GCGC 1 cut(s) 128
Hin1I GRCGYC 1 cut(s) 149
Hin1II CATG 1 cut(s) 86
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HinfI GANTC 3 cut(s) 242, 356, 410
HpaII CCGG 5 cut(s) 285, 290, 315, 360, 405
HphI GGTGA 3 cut(s) 185, 271, 393
Hpy188I TCNGA 2 cut(s) 159, 366
Hpy188III TCNNGA 1 cut(s) 196
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 2 cut(s) 247, 426
HpyCH4III ACNGT 2 cut(s) 60, 382
HpyCH4IV ACGT 2 cut(s) 213, 452
HpyCH4V TGCA 2 cut(s) 249, 422
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 351
HpyF3I CTNAG 2 cut(s) 100, 385
HpySE526I ACGT 2 cut(s) 213, 452
Hsp92I GRCGYC 1 cut(s) 149
Hsp92II CATG 1 cut(s) 86
HspAI GCGC 1 cut(s) 126
Lsp1109I GCAGC 1 cut(s) 31
LweI GCATC 3 cut(s) 242, 258, 398
MaeI CTAG 1 cut(s) 457
MaeII ACGT 2 cut(s) 213, 452
MaeIII GTNAC 1 cut(s) 196
MboII GAAGA 2 cut(s) 25, 28
MlyI GAGTC 2 cut(s) 365, 404
MnlI CCTC 3 cut(s) 319, 322, 356
MspI CCGG 5 cut(s) 285, 290, 315, 360, 405
MspR9I CCNGG 2 cut(s) 290, 405
MwoI GCNNNNNNNGC 2 cut(s) 146, 351
NciI CCSGG 2 cut(s) 290, 405
NlaIII CATG 1 cut(s) 86
NmuCI GTSAC 1 cut(s) 196
NspI RCATGY 1 cut(s) 86
PfeI GAWTC 1 cut(s) 242
PkrI GCNGC 2 cut(s) 46, 70
PleI GAGTC 2 cut(s) 364, 404
PpsI GAGTC 2 cut(s) 364, 404
Ppu21I YACGTR 1 cut(s) 453
PspPI GGNCC 2 cut(s) 108, 287
RsaI GTAC 3 cut(s) 225, 337, 455
RsaNI GTAC 3 cut(s) 224, 336, 454
SatI GCNGC 2 cut(s) 45, 69
Sau96I GGNCC 2 cut(s) 108, 287
SchI GAGTC 2 cut(s) 365, 404
ScrFI CCNGG 2 cut(s) 290, 405
SetI ASST 6 cut(s) 101, 194, 216, 311, 372, 455
SfaNI GCATC 3 cut(s) 242, 258, 398
SinI GGWCC 1 cut(s) 108
SsiI CCGC 3 cut(s) 68, 71, 393
SspMI CTAG 1 cut(s) 457
StyD4I CCNGG 2 cut(s) 288, 403
TaaI ACNGT 2 cut(s) 60, 382
TaiI ACGT 2 cut(s) 216, 455
TaqI TCGA 2 cut(s) 240, 267
TatI WGTACW 1 cut(s) 335
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 1 cut(s) 242
TscAI CASTG 2 cut(s) 65, 356
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 1 cut(s) 44
Tsp45I GTSAC 1 cut(s) 196
TspRI CASTG 2 cut(s) 65, 356
VpaK11BI GGWCC 1 cut(s) 108
XceI RCATGY 1 cut(s) 86
XspI CTAG 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.