Rh1DG249300

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001282
Physical Location & Seq
Reverse (-)
46321314 .. 46330730
9417 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG249300.1

Sequence Viewer

Length: 816 bp
ATGCCGACGGAGACTTTTAAGCAACTCATCAATTCTCTGAGCTTGTATGGGCTTGCAGTTCGTGCCACCGTGACAATTACTCAAATCATGTTCCGTGTAGTCAATGAGGAGGTTGTTCTGAGGGCAGAGGCTGAGGTCTACAAGATTCTGCGTTCGAGAAGTCGATATCCGTATTTGTTGGAATTTGGTCTCCATCAAAAGAGTGCATTCCTGAATGCGGCGAGTCTATCAGACAGAGTTAGGCTTCACCAGCTTTTGGATTTACGCACTGTGTTGGAGATATCAGTAGTGCTCACATACAATTCCATTAGAAGAGAAGAAGAGATCAAGAAAGAAGGAGAAGCCAGAGAGATCGAGATGGAGAGCCAGAAGAAGATGATTGTTGTTTTTGGGTTAGTTGCTGCTGTGTTTTTACAGCGTGTGGCGGCGGAGACATCGCATGTGGTGGGAGATAGCGTAGGCTGGACCATACCACAGAACGGCGCACAAGAGTATGTCACATGGGCGTCTGGTCAGAAGTTCGTAGTTGGTGATGTTCTGGTTTTCAGCTTCGCGACAAGCACACACGACGTGGTGGAAGTACCCAAAGCATCCTTCGACTCTTGCAGCTCCACCAACCCAATCGGCAGCACCATCACCACCGGCCCGGCCAACATCACTCTCACCTCCTCCGGCGAACACTACTACATCTGTACTTTCGGCCTCCACTGCGAGTCCGGCCAGAAGCTCGCCATCACCGTCTCAGCATCCGCCACATCACCCGGAGCCTCCCCTTCTGCACCATCTCTATCTACCAACCCTGACAACACGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

271

Amino Acids

29.66

Weight (kDa)

5.9

Isoelectric Point (pI)

43.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 155 - 241 5.8e-25 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 256
AccI GTMKAC 1 cut(s) 138
AccII CGCG 1 cut(s) 554
AciI CCGC 4 cut(s) 218, 425, 428, 750
AcoI YGGCCR 2 cut(s) 648, 718
AcsI RAATTY 1 cut(s) 182
AcyI GRCGYC 1 cut(s) 506
AdeI CACNNNGTG 1 cut(s) 571
AfaI GTAC 3 cut(s) 582, 694, 812
AfiI CCNNNNNNNGG 3 cut(s) 217, 256, 479
AflIII ACRYGT 1 cut(s) 807
AjiI CACGTC 1 cut(s) 571
AjuI GAANNNNNNNTTGG 2 cut(s) 578, 610
AluBI AGCT 5 cut(s) 42, 253, 549, 609, 727
AluI AGCT 5 cut(s) 42, 253, 549, 609, 727
Alw21I GWGCWC 1 cut(s) 294
Alw26I GTCTC 4 cut(s) 5, 194, 425, 745
AlwNI CAGNNNCTG 1 cut(s) 131
AoxI GGCC 4 cut(s) 643, 648, 700, 718
ApeKI GCWGC 3 cut(s) 401, 606, 627
ApoI RAATTY 1 cut(s) 182
AspLEI GCGC 1 cut(s) 485
AspS9I GGNCC 2 cut(s) 465, 644
AsuC2I CCSGG 2 cut(s) 647, 762
AsuHPI GGTGA 6 cut(s) 239, 542, 628, 655, 727, 750
AvaII GGWCC 1 cut(s) 465
Bbv12I GWGCWC 1 cut(s) 294
BbvCI CCTCAGC 1 cut(s) 132
BbvI GCAGC 3 cut(s) 388, 618, 639
BccI CCATC 5 cut(s) 201, 352, 641, 740, 790
BceAI ACGGC 1 cut(s) 496
BcnI CCSGG 2 cut(s) 647, 762
BcoDI GTCTC 4 cut(s) 5, 194, 425, 745
BfaI CTAG 1 cut(s) 814
BisI GCNGC 5 cut(s) 219, 402, 426, 607, 628
BlsI GCNGC 5 cut(s) 220, 403, 427, 608, 629
Bme1390I CCNGG 2 cut(s) 647, 762
Bme18I GGWCC 1 cut(s) 465
BmgBI CACGTC 1 cut(s) 571
BmgT120I GGNCC 2 cut(s) 465, 644
BmiI GGNNCC 1 cut(s) 766
BmrFI CCNGG 2 cut(s) 647, 762
BmsI GCATC 2 cut(s) 599, 755
Bpu10I CCTNAGC 1 cut(s) 132
BpuMI CCSGG 2 cut(s) 647, 762
BsaAI YACGTR 1 cut(s) 810
BsaHI GRCGYC 1 cut(s) 506
BsaI GGTCTC 1 cut(s) 194
BsaXI ACNNNNNCTCC 2 cut(s) 441, 471
Bsc4I CCNNNNNNNGG 3 cut(s) 217, 256, 479
Bse118I RCCGGY 1 cut(s) 641
BseGI GGATG 2 cut(s) 590, 746
BseLI CCNNNNNNNGG 3 cut(s) 217, 256, 479
BseMII CTCAG 4 cut(s) 29, 110, 123, 756
BseRI GAGGAG 2 cut(s) 122, 658
BseXI GCAGC 3 cut(s) 388, 618, 639
BsgI GTGCAG 1 cut(s) 762
Bsh1236I CGCG 1 cut(s) 554
BshFI GGCC 4 cut(s) 645, 650, 702, 720
BsiHKAI GWGCWC 1 cut(s) 294
BsiSI CCGG 5 cut(s) 642, 647, 672, 717, 762
BslI CCNNNNNNNGG 3 cut(s) 217, 256, 479
BsmAI GTCTC 4 cut(s) 5, 194, 425, 745
BsmBI CGTCTC 1 cut(s) 745
BsmI GAATGC 2 cut(s) 206, 220
BsnI GGCC 4 cut(s) 645, 650, 702, 720
Bso31I GGTCTC 1 cut(s) 194
Bsp1286I GDGCHC 1 cut(s) 294
Bsp143I GATC 2 cut(s) 324, 351
Bsp68I TCGCGA 1 cut(s) 554
BspACI CCGC 4 cut(s) 218, 425, 428, 750
BspANI GGCC 4 cut(s) 645, 650, 702, 720
BspCNI CTCAG 4 cut(s) 30, 111, 124, 755
BspFNI CGCG 1 cut(s) 554
BspLI GGNNCC 1 cut(s) 766
BspTNI GGTCTC 1 cut(s) 194
BsrFI RCCGGY 1 cut(s) 641
BssAI RCCGGY 1 cut(s) 641
BssMI GATC 2 cut(s) 324, 351
BssNI GRCGYC 1 cut(s) 506
Bst4CI ACNGT 3 cut(s) 70, 271, 739
Bst6I CTCTTC 2 cut(s) 307, 315
BstACI GRCGYC 1 cut(s) 506
BstAPI GCANNNNNTGC 1 cut(s) 62
BstBAI YACGTR 1 cut(s) 810
BstC8I GCNNGC 2 cut(s) 54, 729
BstDEI CTNAG 4 cut(s) 38, 119, 132, 742
BstF5I GGATG 2 cut(s) 590, 746
BstFNI CGCG 1 cut(s) 554
BstHHI GCGC 1 cut(s) 485
BstKTI GATC 2 cut(s) 327, 354
BstMAI GTCTC 4 cut(s) 5, 194, 425, 745
BstMBI GATC 2 cut(s) 324, 351
BstMWI GCNNNNNNNGC 4 cut(s) 62, 250, 708, 717
BstNSI RCATGY 1 cut(s) 443
BstSCI CCNGG 2 cut(s) 645, 760
BstUI CGCG 1 cut(s) 554
BstV1I GCAGC 3 cut(s) 388, 618, 639
BsuRI GGCC 4 cut(s) 645, 650, 702, 720
BtgZI GCGATG 1 cut(s) 420
BtrI CACGTC 1 cut(s) 571
BtsCI GGATG 2 cut(s) 590, 746
BtsI GCAGTG 1 cut(s) 706
BtsIMutI CAGTG 2 cut(s) 267, 706
BtuMI TCGCGA 1 cut(s) 554
Cac8I GCNNGC 2 cut(s) 54, 729
CaiI CAGNNNCTG 1 cut(s) 131
CfoI GCGC 1 cut(s) 485
Cfr10I RCCGGY 1 cut(s) 641
Cfr13I GGNCC 2 cut(s) 465, 644
CseI GACGC 1 cut(s) 495
Csp6I GTAC 3 cut(s) 581, 693, 811
CviAII CATG 3 cut(s) 88, 440, 501
CviQI GTAC 3 cut(s) 581, 693, 811
DdeI CTNAG 4 cut(s) 38, 119, 132, 742
DpnI GATC 2 cut(s) 326, 353
DpnII GATC 2 cut(s) 324, 351
DraIII CACNNNGTG 1 cut(s) 571
EaeI YGGCCR 2 cut(s) 648, 718
Eam1104I CTCTTC 2 cut(s) 307, 315
EarI CTCTTC 2 cut(s) 307, 315
EciI GGCGGA 2 cut(s) 443, 739
Eco31I GGTCTC 1 cut(s) 194
Eco32I GATATC 2 cut(s) 167, 282
Eco47I GGWCC 1 cut(s) 465
EcoRV GATATC 2 cut(s) 167, 282
Esp3I CGTCTC 1 cut(s) 745
FaeI CATG 3 cut(s) 91, 443, 504
FaiI YATR 7 cut(s) 48, 89, 298, 441, 470, 495, 502
FatI CATG 3 cut(s) 87, 439, 500
FblI GTMKAC 1 cut(s) 138
Fnu4HI GCNGC 5 cut(s) 219, 402, 426, 607, 628
FokI GGATG 2 cut(s) 577, 733
Fsp4HI GCNGC 5 cut(s) 219, 402, 426, 607, 628
FspBI CTAG 1 cut(s) 814
GlaI GCGC 1 cut(s) 484
GluI GCNGC 5 cut(s) 219, 402, 426, 607, 628
HaeIII GGCC 4 cut(s) 645, 650, 702, 720
HapII CCGG 5 cut(s) 642, 647, 672, 717, 762
HgaI GACGC 1 cut(s) 495
HhaI GCGC 1 cut(s) 485
Hin1I GRCGYC 1 cut(s) 506
Hin1II CATG 3 cut(s) 91, 443, 504
Hin6I GCGC 1 cut(s) 483
HinP1I GCGC 1 cut(s) 483
HinfI GANTC 4 cut(s) 145, 223, 599, 713
HpaII CCGG 5 cut(s) 642, 647, 672, 717, 762
HphI GGTGA 6 cut(s) 239, 542, 628, 655, 727, 750
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 4 cut(s) 39, 120, 232, 516
Hpy188III TCNNGA 5 cut(s) 156, 211, 328, 355, 553
Hpy8I GTNNAC 1 cut(s) 139
Hpy99I CGWCG 2 cut(s) 10, 572
HpyAV CCTTC 3 cut(s) 329, 604, 783
HpyCH4III ACNGT 3 cut(s) 70, 271, 739
HpyCH4IV ACGT 2 cut(s) 570, 809
HpyCH4V TGCA 4 cut(s) 56, 206, 606, 779
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 250, 708, 717
HpyF3I CTNAG 4 cut(s) 38, 119, 132, 742
HpySE526I ACGT 2 cut(s) 570, 809
Hsp92I GRCGYC 1 cut(s) 506
Hsp92II CATG 3 cut(s) 91, 443, 504
HspAI GCGC 1 cut(s) 483
Kzo9I GATC 2 cut(s) 324, 351
LmnI GCTCC 2 cut(s) 614, 764
Lsp1109I GCAGC 3 cut(s) 388, 618, 639
LweI GCATC 2 cut(s) 599, 755
MaeI CTAG 1 cut(s) 814
MaeII ACGT 2 cut(s) 570, 809
MaeIII GTNAC 2 cut(s) 70, 496
MalI GATC 2 cut(s) 326, 353
MboI GATC 2 cut(s) 324, 351
MboII GAAGA 5 cut(s) 324, 329, 332, 382, 385
MhlI GDGCHC 1 cut(s) 294
MluCI AATT 4 cut(s) 31, 75, 182, 301
MlyI GAGTC 3 cut(s) 232, 593, 722
MmeI TCCRAC 2 cut(s) 159, 255
MnlI CCTC 9 cut(s) 100, 103, 114, 121, 127, 676, 679, 713, 778
MseI TTAA 1 cut(s) 18
MspI CCGG 5 cut(s) 642, 647, 672, 717, 762
MspR9I CCNGG 2 cut(s) 647, 762
Mva1269I GAATGC 2 cut(s) 206, 220
MvnI CGCG 1 cut(s) 554
MwoI GCNNNNNNNGC 4 cut(s) 62, 250, 708, 717
NciI CCSGG 2 cut(s) 647, 762
NdeII GATC 2 cut(s) 324, 351
NlaIII CATG 3 cut(s) 91, 443, 504
NlaIV GGNNCC 1 cut(s) 766
NmuCI GTSAC 2 cut(s) 70, 496
NruI TCGCGA 1 cut(s) 554
NspI RCATGY 1 cut(s) 443
PcsI WCGNNNNNNNCGW 1 cut(s) 735
PctI GAATGC 2 cut(s) 206, 220
PfeI GAWTC 1 cut(s) 145
PflMI CCANNNNNTGG 1 cut(s) 256
PkrI GCNGC 5 cut(s) 220, 403, 427, 608, 629
PleI GAGTC 3 cut(s) 231, 593, 721
PpsI GAGTC 3 cut(s) 231, 593, 721
Ppu21I YACGTR 1 cut(s) 810
PspN4I GGNNCC 1 cut(s) 766
PspPI GGNCC 2 cut(s) 465, 644
PstNI CAGNNNCTG 1 cut(s) 131
RruI TCGCGA 1 cut(s) 554
RsaI GTAC 3 cut(s) 582, 694, 812
RsaNI GTAC 3 cut(s) 581, 693, 811
SaqAI TTAA 1 cut(s) 18
SatI GCNGC 5 cut(s) 219, 402, 426, 607, 628
Sau3AI GATC 2 cut(s) 324, 351
Sau96I GGNCC 2 cut(s) 465, 644
SchI GAGTC 3 cut(s) 232, 593, 722
ScrFI CCNGG 2 cut(s) 647, 762
SduI GDGCHC 1 cut(s) 294
SfaNI GCATC 2 cut(s) 599, 755
SinI GGWCC 1 cut(s) 465
Sse9I AATT 4 cut(s) 31, 75, 182, 301
SsiI CCGC 4 cut(s) 218, 425, 428, 750
SspMI CTAG 1 cut(s) 814
StyD4I CCNGG 2 cut(s) 645, 760
TaaI ACNGT 3 cut(s) 70, 271, 739
TaiI ACGT 2 cut(s) 573, 812
TaqI TCGA 4 cut(s) 155, 163, 354, 597
TasI AATT 4 cut(s) 31, 75, 182, 301
TatI WGTACW 1 cut(s) 692
TauI GCSGC 2 cut(s) 221, 428
TfiI GAWTC 1 cut(s) 145
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TscAI CASTG 2 cut(s) 274, 713
TseFI GTSAC 2 cut(s) 70, 496
TseI GCWGC 3 cut(s) 401, 606, 627
Tsp45I GTSAC 2 cut(s) 70, 496
TspGWI ACGGA 3 cut(s) 23, 83, 159
TspRI CASTG 2 cut(s) 274, 713
Van91I CCANNNNNTGG 1 cut(s) 256
VpaK11BI GGWCC 1 cut(s) 465
XapI RAATTY 1 cut(s) 182
XceI RCATGY 1 cut(s) 443
XmiI GTMKAC 1 cut(s) 138
XspI CTAG 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.