RLG00000019417

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001282
Physical Location & Seq
Forward (+)
51769932 .. 51770638
707 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019417

Sequence Viewer

Length: 537 bp
ATGGAGAGCCAGAAGAAGATGATGGTTGTTTTTGGGTTAGTTGCGGCTTTGTTTTTACAGTCTGATGCGGCAGTGACCCCCCATATGATAGACTGGAACATACCACTGACCGGTGCACAAGAGTATAAGACATGGGCGTCTTCTCAGAATTTCGGAGTTGGTGATAGTCTGATTTTCAAGTTCGGGAAAGACGTACACGACGTTACGGAAGTCCCCGAAAAAGTTTACGAAACTTGCAGCTTCGTCAACGATGTGATCAACGCCATCACAACCAGCCCGGCCACCATCATTCTCAACTCCGCCGGCATGCACTACTACACCTCTACTTCGGGCAACAACTGCCAGTCCGGCCAGAAGTTCGCCATCACCGTCTCAGCATCATCCGGCACGTCACCCGGATCCTCCCCTTCTGCACCATCTACATCTACCACAACTAACACCGTGCCGACCGGGGTGCTGCCGAGCTCTTCATCCCCTGCTGCTGGGGCTGGTTTCGTTTTGTGCTCGTTGTCCCTTGTAGTGGGATTGTTCGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

179

Amino Acids

18.51

Weight (kDa)

5.12

Isoelectric Point (pI)

50.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 32 - 118 6.4e-21 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 136
AciI CCGC 3 cut(s) 44, 68, 300
AclWI GGATC 2 cut(s) 393, 406
AcoI YGGCCR 2 cut(s) 279, 349
AcsI RAATTY 1 cut(s) 148
AcyI GRCGYC 1 cut(s) 137
AfaI GTAC 1 cut(s) 195
AfiI CCNNNNNNNGG 3 cut(s) 110, 482, 520
AgeI ACCGGT 1 cut(s) 110
AgsI TTSAA 1 cut(s) 178
AjiI CACGTC 1 cut(s) 390
AluBI AGCT 2 cut(s) 240, 465
AluI AGCT 2 cut(s) 240, 465
Alw21I GWGCWC 3 cut(s) 118, 467, 506
Alw26I GTCTC 1 cut(s) 376
Alw44I GTGCAC 1 cut(s) 114
AlwI GGATC 2 cut(s) 393, 406
AoxI GGCC 2 cut(s) 279, 349
ApaLI GTGCAC 1 cut(s) 114
ApeKI GCWGC 3 cut(s) 237, 457, 479
ApoI RAATTY 1 cut(s) 148
AsiGI ACCGGT 1 cut(s) 110
AsuC2I CCSGG 3 cut(s) 278, 396, 451
AsuHPI GGTGA 3 cut(s) 173, 358, 384
BaeGI GKGCMC 1 cut(s) 118
BamHI GGATCC 1 cut(s) 398
BanII GRGCYC 1 cut(s) 467
BbsI GAAGAC 1 cut(s) 132
Bbv12I GWGCWC 3 cut(s) 118, 467, 506
BbvI GCAGC 3 cut(s) 249, 444, 466
BccI CCATC 5 cut(s) 16, 272, 293, 371, 424
BcgI CGANNNNNNTGC 2 cut(s) 436, 470
BclI TGATCA 1 cut(s) 255
BcnI CCSGG 3 cut(s) 278, 396, 451
BcoDI GTCTC 1 cut(s) 376
BglI GCCNNNNNGGC 1 cut(s) 348
BisI GCNGC 5 cut(s) 45, 69, 238, 458, 480
BlsI GCNGC 5 cut(s) 46, 70, 239, 459, 481
Bme1390I CCNGG 3 cut(s) 278, 396, 451
BmgBI CACGTC 1 cut(s) 390
BmiI GGNNCC 1 cut(s) 400
BmrFI CCNGG 3 cut(s) 278, 396, 451
BmsI GCATC 2 cut(s) 55, 386
BpiI GAAGAC 1 cut(s) 132
BpuMI CCSGG 3 cut(s) 278, 396, 451
BsaHI GRCGYC 1 cut(s) 137
BsaJI CCNNGG 1 cut(s) 450
BsaWI WCCGGW 1 cut(s) 110
Bsc4I CCNNNNNNNGG 3 cut(s) 110, 482, 520
Bse118I RCCGGY 2 cut(s) 110, 302
Bse1I ACTGG 2 cut(s) 98, 343
BseDI CCNNGG 1 cut(s) 450
BseGI GGATG 2 cut(s) 380, 470
BseLI CCNNNNNNNGG 3 cut(s) 110, 482, 520
BseMII CTCAG 2 cut(s) 158, 387
BseNI ACTGG 2 cut(s) 98, 343
BseSI GKGCMC 1 cut(s) 118
BseXI GCAGC 3 cut(s) 249, 444, 466
BseYI CCCAGC 1 cut(s) 482
BsgI GTGCAG 1 cut(s) 396
Bsh1285I CGRYCG 1 cut(s) 450
BshFI GGCC 2 cut(s) 281, 351
BshTI ACCGGT 1 cut(s) 110
BsiEI CGRYCG 1 cut(s) 450
BsiHKAI GWGCWC 3 cut(s) 118, 467, 506
BsiSI CCGG 7 cut(s) 111, 278, 303, 348, 384, 396, 450
BslFI GGGAC 2 cut(s) 197, 496
BslI CCNNNNNNNGG 3 cut(s) 110, 482, 520
BsmAI GTCTC 1 cut(s) 376
BsmBI CGTCTC 1 cut(s) 376
BsmFI GGGAC 2 cut(s) 197, 496
BsnI GGCC 2 cut(s) 281, 351
Bsp1286I GDGCHC 3 cut(s) 118, 467, 506
Bsp143I GATC 2 cut(s) 255, 398
BspACI CCGC 3 cut(s) 44, 68, 300
BspANI GGCC 2 cut(s) 281, 351
BspCNI CTCAG 2 cut(s) 157, 386
BspLI GGNNCC 1 cut(s) 400
BspPI GGATC 2 cut(s) 393, 406
BspQI GCTCTTC 1 cut(s) 472
BsrFI RCCGGY 2 cut(s) 110, 302
BsrI ACTGG 2 cut(s) 98, 343
BssAI RCCGGY 2 cut(s) 110, 302
BssECI CCNNGG 1 cut(s) 450
BssMI GATC 2 cut(s) 255, 398
BssNI GRCGYC 1 cut(s) 137
Bst4CI ACNGT 3 cut(s) 60, 370, 442
Bst6I CTCTTC 1 cut(s) 472
BstACI GRCGYC 1 cut(s) 137
BstAPI GCANNNNNTGC 1 cut(s) 339
BstC8I GCNNGC 2 cut(s) 304, 308
BstDEI CTNAG 2 cut(s) 144, 373
BstF5I GGATG 2 cut(s) 380, 470
BstKTI GATC 2 cut(s) 258, 401
BstMAI GTCTC 1 cut(s) 376
BstMBI GATC 2 cut(s) 255, 398
BstMCI CGRYCG 1 cut(s) 450
BstMWI GCNNNNNNNGC 3 cut(s) 339, 348, 485
BstNSI RCATGY 1 cut(s) 310
BstSCI CCNGG 3 cut(s) 276, 394, 449
BstSLI GKGCMC 1 cut(s) 118
BstV1I GCAGC 3 cut(s) 249, 444, 466
BstV2I GAAGAC 1 cut(s) 132
BstX2I RGATCY 1 cut(s) 398
BstYI RGATCY 1 cut(s) 398
BsuRI GGCC 2 cut(s) 281, 351
BtrI CACGTC 1 cut(s) 390
BtsCI GGATG 2 cut(s) 380, 470
BtsI GCAGTG 1 cut(s) 78
BtsIMutI CAGTG 2 cut(s) 78, 104
Cac8I GCNNGC 2 cut(s) 304, 308
Cfr10I RCCGGY 2 cut(s) 110, 302
CseI GACGC 1 cut(s) 126
Csp6I GTAC 1 cut(s) 194
CspAI ACCGGT 1 cut(s) 110
CviAII CATG 2 cut(s) 132, 307
CviJI RGCY 8 cut(s) 9, 47, 240, 276, 281, 351, 465, 488
CviKI_1 RGCY 8 cut(s) 9, 47, 240, 276, 281, 351, 465, 488
CviQI GTAC 1 cut(s) 194
DdeI CTNAG 2 cut(s) 144, 373
DpnI GATC 2 cut(s) 257, 400
DpnII GATC 2 cut(s) 255, 398
DrdI GACNNNNNNGTC 1 cut(s) 136
DseDI GACNNNNNNGTC 1 cut(s) 136
EaeI YGGCCR 2 cut(s) 279, 349
Eam1104I CTCTTC 1 cut(s) 472
EarI CTCTTC 1 cut(s) 472
EciI GGCGGA 1 cut(s) 289
Ecl136II GAGCTC 1 cut(s) 465
Eco24I GRGCYC 1 cut(s) 467
Eco53kI GAGCTC 1 cut(s) 465
EcoICRI GAGCTC 1 cut(s) 465
EcoT38I GRGCYC 1 cut(s) 467
Esp3I CGTCTC 1 cut(s) 376
FaeI CATG 2 cut(s) 135, 310
FaiI YATR 6 cut(s) 84, 86, 101, 126, 133, 308
FaqI GGGAC 2 cut(s) 197, 496
FatI CATG 2 cut(s) 131, 306
FauNDI CATATG 1 cut(s) 84
FbaI TGATCA 1 cut(s) 255
Fnu4HI GCNGC 5 cut(s) 45, 69, 238, 458, 480
FokI GGATG 2 cut(s) 367, 457
FriOI GRGCYC 1 cut(s) 467
Fsp4HI GCNGC 5 cut(s) 45, 69, 238, 458, 480
GluI GCNGC 5 cut(s) 45, 69, 238, 458, 480
GsaI CCCAGC 1 cut(s) 486
HaeIII GGCC 2 cut(s) 281, 351
HapII CCGG 7 cut(s) 111, 278, 303, 348, 384, 396, 450
HgaI GACGC 1 cut(s) 126
Hin1I GRCGYC 1 cut(s) 137
Hin1II CATG 2 cut(s) 135, 310
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HpaII CCGG 7 cut(s) 111, 278, 303, 348, 384, 396, 450
HphI GGTGA 3 cut(s) 173, 358, 384
Hpy166II GTNNAC 4 cut(s) 116, 196, 226, 247
Hpy188I TCNGA 4 cut(s) 64, 147, 155, 171
Hpy188III TCNNGA 1 cut(s) 184
Hpy8I GTNNAC 4 cut(s) 116, 196, 226, 247
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 1 cut(s) 417
HpyCH4III ACNGT 3 cut(s) 60, 370, 442
HpyCH4IV ACGT 3 cut(s) 192, 201, 389
HpyCH4V TGCA 4 cut(s) 116, 237, 310, 413
HpyF10VI GCNNNNNNNGC 3 cut(s) 339, 348, 485
HpyF3I CTNAG 2 cut(s) 144, 373
HpySE526I ACGT 3 cut(s) 192, 201, 389
Hsp92I GRCGYC 1 cut(s) 137
Hsp92II CATG 2 cut(s) 135, 310
KroI GCCGGC 1 cut(s) 302
KroNI GCCGGC 1 cut(s) 304
Ksp22I TGATCA 1 cut(s) 255
Kzo9I GATC 2 cut(s) 255, 398
LguI GCTCTTC 1 cut(s) 472
Lsp1109I GCAGC 3 cut(s) 249, 444, 466
LweI GCATC 2 cut(s) 55, 386
MaeII ACGT 3 cut(s) 192, 201, 389
MaeIII GTNAC 3 cut(s) 73, 202, 390
MalI GATC 2 cut(s) 257, 400
MboI GATC 2 cut(s) 255, 398
MboII GAAGA 4 cut(s) 25, 28, 132, 459
MflI RGATCY 1 cut(s) 398
MhlI GDGCHC 3 cut(s) 118, 467, 506
MluCI AATT 1 cut(s) 148
MnlI CCTC 2 cut(s) 331, 412
MroNI GCCGGC 1 cut(s) 302
MspI CCGG 7 cut(s) 111, 278, 303, 348, 384, 396, 450
MspR9I CCNGG 3 cut(s) 278, 396, 451
MwoI GCNNNNNNNGC 3 cut(s) 339, 348, 485
NaeI GCCGGC 1 cut(s) 304
NciI CCSGG 3 cut(s) 278, 396, 451
NdeI CATATG 1 cut(s) 84
NdeII GATC 2 cut(s) 255, 398
NgoMIV GCCGGC 1 cut(s) 302
NlaIII CATG 2 cut(s) 135, 310
NlaIV GGNNCC 1 cut(s) 400
NmeAIII GCCGAG 1 cut(s) 486
NmuCI GTSAC 2 cut(s) 73, 390
NspI RCATGY 1 cut(s) 310
PaeI GCATGC 1 cut(s) 310
PciSI GCTCTTC 1 cut(s) 472
PcsI WCGNNNNNNNCGW 3 cut(s) 189, 198, 366
PdiI GCCGGC 1 cut(s) 304
PinAI ACCGGT 1 cut(s) 110
PkrI GCNGC 5 cut(s) 46, 70, 239, 459, 481
Psp124BI GAGCTC 1 cut(s) 467
PspFI CCCAGC 1 cut(s) 482
PspN4I GGNNCC 1 cut(s) 400
PsuI RGATCY 1 cut(s) 398
RsaI GTAC 1 cut(s) 195
RsaNI GTAC 1 cut(s) 194
SacI GAGCTC 1 cut(s) 467
SapI GCTCTTC 1 cut(s) 472
SatI GCNGC 5 cut(s) 45, 69, 238, 458, 480
Sau3AI GATC 2 cut(s) 255, 398
ScrFI CCNGG 3 cut(s) 278, 396, 451
SduI GDGCHC 3 cut(s) 118, 467, 506
SetI ASST 6 cut(s) 195, 204, 242, 323, 392, 467
SfaNI GCATC 2 cut(s) 55, 386
SphI GCATGC 1 cut(s) 310
Sse9I AATT 1 cut(s) 148
SsiI CCGC 3 cut(s) 44, 68, 300
SstI GAGCTC 1 cut(s) 467
StyD4I CCNGG 3 cut(s) 276, 394, 449
TaaI ACNGT 3 cut(s) 60, 370, 442
TaiI ACGT 3 cut(s) 195, 204, 392
TasI AATT 1 cut(s) 148
TauI GCSGC 2 cut(s) 47, 71
TscAI CASTG 2 cut(s) 78, 111
TseFI GTSAC 2 cut(s) 73, 390
TseI GCWGC 3 cut(s) 237, 457, 479
Tsp45I GTSAC 2 cut(s) 73, 390
TspDTI ATGAA 1 cut(s) 459
TspGWI ACGGA 1 cut(s) 221
TspRI CASTG 2 cut(s) 78, 111
VneI GTGCAC 1 cut(s) 114
XapI RAATTY 1 cut(s) 148
XceI RCATGY 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.