Rh1BG222100

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0001282
Physical Location & Seq
Reverse (-)
34139599 .. 34140175
577 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG222100.1

Sequence Viewer

Length: 444 bp
ATGGAGAGCCAGAAGAAGATGATTGTTGTTTTTGGGTTAGTTGCTGCTGTGTTTTTACAGTGTGTGGCGGCGGAGACATCGCATGTGGTGGGAGATAGCGTAGGCTGGACCATACCACAGAACGGCGCACAAGAGTATGTCACATGGGCGTCTGGTCAGAAGTTCGTAGTTGGTGATGTTCTGGTTTTCAGCTTCGCGACAAGCACACACGACGTGGTGGAAGTACCCAAAGCATCCTTCGACTCTTGCAGCTCCACCAACCCAATCGGCAGCACCATCACCACCGGCCCGGCCAACATCACTCTCACCTCCTCCGGCGAACACTACTACATCTGTACTTTCGGCCTCCACTGCGAGTCCGGCCAGAAGCTCGCCATCACCGTCTCAGCATCCGCCTCCCCTTCTGCACCATCTCTATCTACCAACCCTGACAACACGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

147

Amino Acids

15.39

Weight (kDa)

4.88

Isoelectric Point (pI)

34.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 36 - 122 1.4e-25 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 197
AciI CCGC 3 cut(s) 68, 71, 393
AcoI YGGCCR 2 cut(s) 291, 361
AcyI GRCGYC 1 cut(s) 149
AdeI CACNNNGTG 1 cut(s) 214
AfaI GTAC 3 cut(s) 225, 337, 440
AfiI CCNNNNNNNGG 1 cut(s) 122
AflIII ACRYGT 1 cut(s) 435
AjiI CACGTC 1 cut(s) 214
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AluBI AGCT 3 cut(s) 192, 252, 370
AluI AGCT 3 cut(s) 192, 252, 370
Alw26I GTCTC 2 cut(s) 68, 388
AoxI GGCC 4 cut(s) 286, 291, 343, 361
ApeKI GCWGC 3 cut(s) 44, 249, 270
AspLEI GCGC 1 cut(s) 128
AspS9I GGNCC 2 cut(s) 108, 287
AsuC2I CCSGG 1 cut(s) 290
AsuHPI GGTGA 4 cut(s) 185, 271, 298, 370
AvaII GGWCC 1 cut(s) 108
BbvI GCAGC 3 cut(s) 31, 261, 282
BccI CCATC 3 cut(s) 284, 383, 418
BceAI ACGGC 1 cut(s) 139
BcnI CCSGG 1 cut(s) 290
BcoDI GTCTC 2 cut(s) 68, 388
BfaI CTAG 1 cut(s) 442
BisI GCNGC 4 cut(s) 45, 69, 250, 271
BlsI GCNGC 4 cut(s) 46, 70, 251, 272
Bme1390I CCNGG 1 cut(s) 290
Bme18I GGWCC 1 cut(s) 108
BmgBI CACGTC 1 cut(s) 214
BmgT120I GGNCC 2 cut(s) 108, 287
BmrFI CCNGG 1 cut(s) 290
BmsI GCATC 2 cut(s) 242, 398
BpuMI CCSGG 1 cut(s) 290
BsaAI YACGTR 1 cut(s) 438
BsaHI GRCGYC 1 cut(s) 149
BsaXI ACNNNNNCTCC 2 cut(s) 84, 114
Bsc4I CCNNNNNNNGG 1 cut(s) 122
Bse118I RCCGGY 1 cut(s) 284
BseGI GGATG 2 cut(s) 233, 389
BseLI CCNNNNNNNGG 1 cut(s) 122
BseMII CTCAG 1 cut(s) 399
BseRI GAGGAG 1 cut(s) 301
BseXI GCAGC 3 cut(s) 31, 261, 282
BsgI GTGCAG 1 cut(s) 390
Bsh1236I CGCG 1 cut(s) 197
BshFI GGCC 4 cut(s) 288, 293, 345, 363
BsiSI CCGG 4 cut(s) 285, 290, 315, 360
BslI CCNNNNNNNGG 1 cut(s) 122
BsmAI GTCTC 2 cut(s) 68, 388
BsmBI CGTCTC 1 cut(s) 388
BsnI GGCC 4 cut(s) 288, 293, 345, 363
Bsp68I TCGCGA 1 cut(s) 197
BspACI CCGC 3 cut(s) 68, 71, 393
BspANI GGCC 4 cut(s) 288, 293, 345, 363
BspCNI CTCAG 1 cut(s) 398
BspFNI CGCG 1 cut(s) 197
BsrFI RCCGGY 1 cut(s) 284
BssAI RCCGGY 1 cut(s) 284
BssNI GRCGYC 1 cut(s) 149
Bst4CI ACNGT 2 cut(s) 60, 382
BstACI GRCGYC 1 cut(s) 149
BstBAI YACGTR 1 cut(s) 438
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 1 cut(s) 385
BstF5I GGATG 2 cut(s) 233, 389
BstFNI CGCG 1 cut(s) 197
BstHHI GCGC 1 cut(s) 128
BstMAI GTCTC 2 cut(s) 68, 388
BstMWI GCNNNNNNNGC 2 cut(s) 351, 360
BstNSI RCATGY 1 cut(s) 86
BstSCI CCNGG 1 cut(s) 288
BstUI CGCG 1 cut(s) 197
BstV1I GCAGC 3 cut(s) 31, 261, 282
BsuRI GGCC 4 cut(s) 288, 293, 345, 363
BtgZI GCGATG 1 cut(s) 63
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 2 cut(s) 233, 389
BtsI GCAGTG 1 cut(s) 349
BtsIMutI CAGTG 2 cut(s) 65, 349
BtuMI TCGCGA 1 cut(s) 197
Cac8I GCNNGC 1 cut(s) 372
CfoI GCGC 1 cut(s) 128
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 2 cut(s) 108, 287
CseI GACGC 1 cut(s) 138
Csp6I GTAC 3 cut(s) 224, 336, 439
CviAII CATG 2 cut(s) 83, 144
CviJI RGCY 9 cut(s) 9, 105, 192, 252, 288, 293, 345, 363, 370
CviKI_1 RGCY 9 cut(s) 9, 105, 192, 252, 288, 293, 345, 363, 370
CviQI GTAC 3 cut(s) 224, 336, 439
DdeI CTNAG 1 cut(s) 385
DraIII CACNNNGTG 1 cut(s) 214
EaeI YGGCCR 2 cut(s) 291, 361
EciI GGCGGA 2 cut(s) 86, 382
Eco47I GGWCC 1 cut(s) 108
Esp3I CGTCTC 1 cut(s) 388
FaeI CATG 2 cut(s) 86, 147
FaiI YATR 4 cut(s) 84, 113, 138, 145
FatI CATG 2 cut(s) 82, 143
Fnu4HI GCNGC 4 cut(s) 45, 69, 250, 271
FokI GGATG 2 cut(s) 220, 376
Fsp4HI GCNGC 4 cut(s) 45, 69, 250, 271
FspBI CTAG 1 cut(s) 442
GlaI GCGC 1 cut(s) 127
GluI GCNGC 4 cut(s) 45, 69, 250, 271
HaeIII GGCC 4 cut(s) 288, 293, 345, 363
HapII CCGG 4 cut(s) 285, 290, 315, 360
HgaI GACGC 1 cut(s) 138
HhaI GCGC 1 cut(s) 128
Hin1I GRCGYC 1 cut(s) 149
Hin1II CATG 2 cut(s) 86, 147
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HinfI GANTC 2 cut(s) 242, 356
HpaII CCGG 4 cut(s) 285, 290, 315, 360
HphI GGTGA 4 cut(s) 185, 271, 298, 370
Hpy188I TCNGA 1 cut(s) 159
Hpy188III TCNNGA 1 cut(s) 196
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 2 cut(s) 247, 411
HpyCH4III ACNGT 2 cut(s) 60, 382
HpyCH4IV ACGT 2 cut(s) 213, 437
HpyCH4V TGCA 2 cut(s) 249, 407
HpyF10VI GCNNNNNNNGC 2 cut(s) 351, 360
HpyF3I CTNAG 1 cut(s) 385
HpySE526I ACGT 2 cut(s) 213, 437
Hsp92I GRCGYC 1 cut(s) 149
Hsp92II CATG 2 cut(s) 86, 147
HspAI GCGC 1 cut(s) 126
LmnI GCTCC 1 cut(s) 257
LpnPI CCDG 9 cut(s) 23, 91, 138, 167, 298, 303, 328, 373, 377
Lsp1109I GCAGC 3 cut(s) 31, 261, 282
LweI GCATC 2 cut(s) 242, 398
MaeI CTAG 1 cut(s) 442
MaeII ACGT 2 cut(s) 213, 437
MaeIII GTNAC 1 cut(s) 139
MboII GAAGA 2 cut(s) 25, 28
MlyI GAGTC 2 cut(s) 236, 365
MnlI CCTC 4 cut(s) 319, 322, 356, 406
MspI CCGG 4 cut(s) 285, 290, 315, 360
MspR9I CCNGG 1 cut(s) 290
MvnI CGCG 1 cut(s) 197
MwoI GCNNNNNNNGC 2 cut(s) 351, 360
NciI CCSGG 1 cut(s) 290
NlaIII CATG 2 cut(s) 86, 147
NmuCI GTSAC 1 cut(s) 139
NruI TCGCGA 1 cut(s) 197
NspI RCATGY 1 cut(s) 86
PcsI WCGNNNNNNNCGW 1 cut(s) 378
PkrI GCNGC 4 cut(s) 46, 70, 251, 272
PleI GAGTC 2 cut(s) 236, 364
PpsI GAGTC 2 cut(s) 236, 364
Ppu21I YACGTR 1 cut(s) 438
PspPI GGNCC 2 cut(s) 108, 287
RruI TCGCGA 1 cut(s) 197
RsaI GTAC 3 cut(s) 225, 337, 440
RsaNI GTAC 3 cut(s) 224, 336, 439
SatI GCNGC 4 cut(s) 45, 69, 250, 271
Sau96I GGNCC 2 cut(s) 108, 287
SchI GAGTC 2 cut(s) 236, 365
ScrFI CCNGG 1 cut(s) 290
SetI ASST 6 cut(s) 194, 216, 254, 311, 372, 440
SfaNI GCATC 2 cut(s) 242, 398
SinI GGWCC 1 cut(s) 108
SsiI CCGC 3 cut(s) 68, 71, 393
SspMI CTAG 1 cut(s) 442
StyD4I CCNGG 1 cut(s) 288
TaaI ACNGT 2 cut(s) 60, 382
TaiI ACGT 2 cut(s) 216, 440
TaqI TCGA 1 cut(s) 240
TatI WGTACW 1 cut(s) 335
TauI GCSGC 1 cut(s) 71
TscAI CASTG 2 cut(s) 65, 356
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 3 cut(s) 44, 249, 270
Tsp45I GTSAC 1 cut(s) 139
TspRI CASTG 2 cut(s) 65, 356
VpaK11BI GGWCC 1 cut(s) 108
XceI RCATGY 1 cut(s) 86
XspI CTAG 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.