RLG00000019470

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001282
Physical Location & Seq
Reverse (-)
52400773 .. 52408042
7270 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019470

Sequence Viewer

Length: 600 bp
ATGGAGAGCCAGAAGAAGACGATGAAGATTGTGGCAGCGCAGACGGCGCATGTGGTGGGAGGTAGCATAGGCTGGACCATACCACAGAACGGTGCTCAAGAGTATTTCACTTGGGCGTCTGGACAGAAGTTCCTGGTTGGTGATGTTCTGAGAAAACTTAACCCCCTTTTCGAAAATCCCAACGGAAATAACATCTCCTCCAACCCAATCAGCAGCACCATCACCATTGGTCCAACCAACGTTACTCTCACGTTTGCTGGCGACCACTATTATATCTGCACTCTCGGCAGCCATTGCCAGTCTGGACAAAAGATTGCCATTACCTTCTCAGCATCAGTTACATCACCTGGGGCCTCCCCTTCTGCACCAACTCCCGTATCCCCAATGCCACCAACTGCCACTACTACTCTTACCACACCTTCACCCACATCTAACGCCCCTTGCCCTCTAGTCCAAGCACCATCTCTGTCCACCAGAACCACCTGGTCCACAACTAGCACCACGCCGGGATCCACTGCGTTGCCCCCAAGCTCTTCTTCCCCTAACGTTAGGGCTGACTACCATCTTTATGATGGTGATGCATGCTGTGTACAAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

200

Amino Acids

20.69

Weight (kDa)

7.66

Isoelectric Point (pI)

46.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 25 - 103 3.1e-09 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 2 cut(s) 240, 546
AclWI GGATC 2 cut(s) 504, 517
AcyI GRCGYC 1 cut(s) 116
AfaI GTAC 1 cut(s) 591
AfiI CCNNNNNNNGG 1 cut(s) 89
AjnI CCWGG 3 cut(s) 132, 346, 482
AjuI GAANNNNNNNTTGG 2 cut(s) 520, 552
AluBI AGCT 1 cut(s) 531
AluI AGCT 1 cut(s) 531
Alw21I GWGCWC 1 cut(s) 97
AlwI GGATC 2 cut(s) 504, 517
AoxI GGCC 1 cut(s) 351
ApeKI GCWGC 3 cut(s) 35, 213, 288
AspLEI GCGC 2 cut(s) 40, 49
AspS9I GGNCC 4 cut(s) 75, 230, 351, 486
AsuC2I CCSGG 1 cut(s) 507
AsuHPI GGTGA 5 cut(s) 152, 214, 336, 414, 587
AsuII TTCGAA 1 cut(s) 171
AvaII GGWCC 3 cut(s) 75, 230, 486
BaeI ACNNNNGTAYC 2 cut(s) 360, 393
BamHI GGATCC 1 cut(s) 509
BbsI GAAGAC 1 cut(s) 23
Bbv12I GWGCWC 1 cut(s) 97
BbvI GCAGC 3 cut(s) 47, 225, 300
BccI CCATC 4 cut(s) 227, 469, 566, 570
BceAI ACGGC 1 cut(s) 60
BciT130I CCWGG 3 cut(s) 134, 348, 484
BciVI GTATCC 1 cut(s) 388
BcnI CCSGG 1 cut(s) 507
BfaI CTAG 2 cut(s) 449, 495
BfuI GTATCC 1 cut(s) 388
BisI GCNGC 3 cut(s) 36, 214, 289
BlsI GCNGC 3 cut(s) 37, 215, 290
Bme1390I CCNGG 4 cut(s) 134, 348, 484, 507
Bme18I GGWCC 3 cut(s) 75, 230, 486
BmgT120I GGNCC 4 cut(s) 75, 230, 351, 486
BmiI GGNNCC 2 cut(s) 352, 511
BmrFI CCNGG 4 cut(s) 134, 348, 484, 507
BmsI GCATC 2 cut(s) 341, 568
BpiI GAAGAC 1 cut(s) 23
Bpu14I TTCGAA 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 81
BpuMI CCSGG 1 cut(s) 507
BsaHI GRCGYC 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 347
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 1 cut(s) 89
Bse1I ACTGG 1 cut(s) 298
Bse3DI GCAATG 1 cut(s) 292
BseBI CCWGG 3 cut(s) 134, 348, 484
BseDI CCNNGG 1 cut(s) 347
BseLI CCNNNNNNNGG 1 cut(s) 89
BseMI GCAATG 1 cut(s) 292
BseMII CTCAG 2 cut(s) 140, 342
BseNI ACTGG 1 cut(s) 298
BseRI GAGGAG 1 cut(s) 187
BseXI GCAGC 3 cut(s) 47, 225, 300
BsgI GTGCAG 2 cut(s) 262, 348
BshFI GGCC 1 cut(s) 353
BsiHKAI GWGCWC 1 cut(s) 97
BsiSI CCGG 1 cut(s) 506
BslI CCNNNNNNNGG 1 cut(s) 89
BsnI GGCC 1 cut(s) 353
Bsp119I TTCGAA 1 cut(s) 171
Bsp1286I GDGCHC 1 cut(s) 97
Bsp1407I TGTACA 1 cut(s) 589
Bsp143I GATC 1 cut(s) 509
BspANI GGCC 1 cut(s) 353
BspCNI CTCAG 2 cut(s) 141, 341
BspLI GGNNCC 2 cut(s) 352, 511
BspPI GGATC 2 cut(s) 504, 517
BspQI GCTCTTC 1 cut(s) 538
BspT104I TTCGAA 1 cut(s) 171
BsrDI GCAATG 1 cut(s) 292
BsrGI TGTACA 1 cut(s) 589
BsrI ACTGG 1 cut(s) 298
BssECI CCNNGG 1 cut(s) 347
BssMI GATC 1 cut(s) 509
BssNI GRCGYC 1 cut(s) 116
Bst2UI CCWGG 3 cut(s) 134, 348, 484
Bst4CI ACNGT 1 cut(s) 92
Bst6I CTCTTC 1 cut(s) 538
BstACI GRCGYC 1 cut(s) 116
BstAPI GCANNNNNTGC 1 cut(s) 294
BstAUI TGTACA 1 cut(s) 589
BstBI TTCGAA 1 cut(s) 171
BstC8I GCNNGC 2 cut(s) 259, 583
BstDEI CTNAG 2 cut(s) 149, 328
BstHHI GCGC 2 cut(s) 40, 49
BstKTI GATC 1 cut(s) 512
BstMBI GATC 1 cut(s) 509
BstMWI GCNNNNNNNGC 4 cut(s) 44, 46, 285, 294
BstNI CCWGG 3 cut(s) 134, 348, 484
BstNSI RCATGY 2 cut(s) 53, 585
BstSCI CCNGG 4 cut(s) 132, 346, 482, 505
BstV1I GCAGC 3 cut(s) 47, 225, 300
BstV2I GAAGAC 1 cut(s) 23
BstX2I RGATCY 1 cut(s) 509
BstYI RGATCY 1 cut(s) 509
BsuI GTATCC 1 cut(s) 388
BsuRI GGCC 1 cut(s) 353
BtsI GCAGTG 1 cut(s) 513
BtsIMutI CAGTG 1 cut(s) 513
Cac8I GCNNGC 2 cut(s) 259, 583
CfoI GCGC 2 cut(s) 40, 49
Cfr13I GGNCC 4 cut(s) 75, 230, 351, 486
CseI GACGC 1 cut(s) 105
CsiI ACCWGGT 1 cut(s) 482
Csp6I GTAC 1 cut(s) 590
CviAII CATG 2 cut(s) 50, 582
CviJI RGCY 6 cut(s) 9, 72, 291, 353, 531, 554
CviKI_1 RGCY 6 cut(s) 9, 72, 291, 353, 531, 554
CviQI GTAC 1 cut(s) 590
DdeI CTNAG 2 cut(s) 149, 328
DpnI GATC 1 cut(s) 511
DpnII GATC 1 cut(s) 509
Eam1104I CTCTTC 1 cut(s) 538
EarI CTCTTC 1 cut(s) 538
Eco47I GGWCC 3 cut(s) 75, 230, 486
EcoO109I RGGNCCY 1 cut(s) 351
EcoRII CCWGG 3 cut(s) 132, 346, 482
EcoT22I ATGCAT 1 cut(s) 583
FaeI CATG 2 cut(s) 53, 585
FaiI YATR 7 cut(s) 51, 68, 80, 273, 570, 583, 598
FalI AAGNNNNNCTT 2 cut(s) 520, 552
FatI CATG 2 cut(s) 49, 581
Fnu4HI GCNGC 3 cut(s) 36, 214, 289
Fsp4HI GCNGC 3 cut(s) 36, 214, 289
FspBI CTAG 2 cut(s) 449, 495
GlaI GCGC 2 cut(s) 39, 48
GluI GCNGC 3 cut(s) 36, 214, 289
HaeIII GGCC 1 cut(s) 353
HapII CCGG 1 cut(s) 506
HgaI GACGC 1 cut(s) 105
HhaI GCGC 2 cut(s) 40, 49
Hin1I GRCGYC 1 cut(s) 116
Hin1II CATG 2 cut(s) 53, 585
Hin6I GCGC 2 cut(s) 38, 47
HinP1I GCGC 2 cut(s) 38, 47
HpaII CCGG 1 cut(s) 506
HphI GGTGA 5 cut(s) 152, 214, 336, 414, 587
Hpy166II GTNNAC 3 cut(s) 471, 489, 590
Hpy188I TCNGA 1 cut(s) 150
Hpy188III TCNNGA 3 cut(s) 98, 120, 303
Hpy8I GTNNAC 3 cut(s) 471, 489, 590
HpyAV CCTTC 3 cut(s) 334, 369, 429
HpyCH4III ACNGT 1 cut(s) 92
HpyCH4IV ACGT 3 cut(s) 240, 251, 546
HpyCH4V TGCA 3 cut(s) 279, 365, 581
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 46, 285, 294
HpyF3I CTNAG 2 cut(s) 149, 328
HpySE526I ACGT 3 cut(s) 240, 251, 546
Hsp92I GRCGYC 1 cut(s) 116
Hsp92II CATG 2 cut(s) 53, 585
HspAI GCGC 2 cut(s) 38, 47
Kzo9I GATC 1 cut(s) 509
LguI GCTCTTC 1 cut(s) 538
Lsp1109I GCAGC 3 cut(s) 47, 225, 300
LweI GCATC 2 cut(s) 341, 568
MabI ACCWGGT 1 cut(s) 482
MaeI CTAG 2 cut(s) 449, 495
MaeII ACGT 3 cut(s) 240, 251, 546
MaeIII GTNAC 2 cut(s) 241, 337
MalI GATC 1 cut(s) 511
MboI GATC 1 cut(s) 509
MboII GAAGA 5 cut(s) 25, 28, 37, 525, 528
MflI RGATCY 1 cut(s) 509
MhlI GDGCHC 1 cut(s) 97
MmeI TCCRAC 2 cut(s) 225, 257
MnlI CCTC 4 cut(s) 53, 208, 364, 456
Mph1103I ATGCAT 1 cut(s) 583
MseI TTAA 1 cut(s) 159
MslI CAYNNNNRTG 1 cut(s) 567
MspI CCGG 1 cut(s) 506
MspR9I CCNGG 4 cut(s) 134, 348, 484, 507
MvaI CCWGG 3 cut(s) 134, 348, 484
MwoI GCNNNNNNNGC 4 cut(s) 44, 46, 285, 294
NciI CCSGG 1 cut(s) 507
NdeII GATC 1 cut(s) 509
NlaIII CATG 2 cut(s) 53, 585
NlaIV GGNNCC 2 cut(s) 352, 511
NmeAIII GCCGAG 1 cut(s) 264
NsiI ATGCAT 1 cut(s) 583
NspI RCATGY 2 cut(s) 53, 585
NspV TTCGAA 1 cut(s) 171
PaeI GCATGC 1 cut(s) 585
PciSI GCTCTTC 1 cut(s) 538
PkrI GCNGC 3 cut(s) 37, 215, 290
Psp1406I AACGTT 2 cut(s) 240, 546
Psp6I CCWGG 3 cut(s) 132, 346, 482
PspGI CCWGG 3 cut(s) 132, 346, 482
PspN4I GGNNCC 2 cut(s) 352, 511
PspPI GGNCC 4 cut(s) 75, 230, 351, 486
PsuI RGATCY 1 cut(s) 509
RsaI GTAC 1 cut(s) 591
RsaNI GTAC 1 cut(s) 590
RseI CAYNNNNRTG 1 cut(s) 567
SapI GCTCTTC 1 cut(s) 538
SaqAI TTAA 1 cut(s) 159
SatI GCNGC 3 cut(s) 36, 214, 289
Sau3AI GATC 1 cut(s) 509
Sau96I GGNCC 4 cut(s) 75, 230, 351, 486
ScrFI CCNGG 4 cut(s) 134, 348, 484, 507
SduI GDGCHC 1 cut(s) 97
SetI ASST 9 cut(s) 64, 243, 254, 326, 349, 421, 485, 533, 549
SexAI ACCWGGT 1 cut(s) 482
SfaNI GCATC 2 cut(s) 341, 568
SfuI TTCGAA 1 cut(s) 171
SinI GGWCC 3 cut(s) 75, 230, 486
SmiMI CAYNNNNRTG 1 cut(s) 567
SmlI CTYRAG 1 cut(s) 96
SmoI CTYRAG 1 cut(s) 96
SphI GCATGC 1 cut(s) 585
SspMI CTAG 2 cut(s) 449, 495
StyD4I CCNGG 4 cut(s) 132, 346, 482, 505
TaaI ACNGT 1 cut(s) 92
TaiI ACGT 3 cut(s) 243, 254, 549
TaqI TCGA 1 cut(s) 171
TatI WGTACW 1 cut(s) 589
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 1 cut(s) 520
TseI GCWGC 3 cut(s) 35, 213, 288
TspDTI ATGAA 1 cut(s) 38
TspGWI ACGGA 1 cut(s) 198
TspRI CASTG 1 cut(s) 520
VpaK11BI GGWCC 3 cut(s) 75, 230, 486
XceI RCATGY 2 cut(s) 53, 585
XcmI CCANNNNNNNNNTGG 2 cut(s) 299, 569
XspI CTAG 2 cut(s) 449, 495
Zsp2I ATGCAT 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.