Rh2CG362200

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0001282
Physical Location & Seq
Reverse (-)
49063465 .. 49068186
4722 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG362200.1

Sequence Viewer

Length: 777 bp
ATGGAAAACCAGAAGAAGACGATGGTTGTTTTTGGGTTACTTGCGGCTGTGTTTTTGGAGAGTGTGGCGGCGCAGACGGCCCATGTGGTGGGAGGTAGCGTAGGCTGGACCATACCACAGAATGGTGCACAAGAGTATGTCACTTGGGCGTCCGGTCAGAAGTTCATAGTTGGTGATGTTCTAACCTTCAACTTCGCGACAGACGCACACGACGTAGTGCAAGTACCCAAAGCATCATTCGACTCATGCAGCTCTGCCAACCCAATCGGCAGTACCTTCACCACGGGCCCGGCCAACATCACTCTCACATCTGCCGGCAACCACTACTACATCTGCACTTTCGGCCGCCACTGCCAGTCCGGCCAGAAGCTCGCCATCACCGTCTCAGCATCCGCCATGTCACCGGGAGCCTCCCCTTCTGCACCAACTCCTATACCTCCACCTCCACCCACTACCAGTACTACTCCTACCACGCCTTCACCCACATCTGATGCCCCTTGCCCTCCTGTCCAAGCACCATCTCCGTCCACCAAAACCACGGGGCCCACAGCCAGCACCACACCAGGATCCACCGTGTTGCCGCCGAGCTCTTCTTCCCCTGCCGTTGGGGCTGAAATAGATTGGGGAAGAGCTAGGGCACTGACCTTTGGCATTGGGTTGGAGAAGATGTTCATGGGGAAGAACAAGTCAAAACTACCAAAATTTAAACTTGAGGAGAGGTCGAACGAAGGGCCTGAACTCGTCGGATCCGCGGCTCGGGAGGCTTATGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

258

Amino Acids

26.64

Weight (kDa)

6.42

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 36 - 122 1.5e-25 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 88, 122
AccII CGCG 2 cut(s) 197, 752
AciI CCGC 7 cut(s) 44, 68, 346, 393, 581, 750, 752
AclWI GGATC 4 cut(s) 561, 574, 741, 754
AcoI YGGCCR 3 cut(s) 291, 343, 361
AcsI RAATTY 1 cut(s) 701
AcyI GRCGYC 1 cut(s) 149
AfaI GTAC 3 cut(s) 225, 274, 460
AfiI CCNNNNNNNGG 4 cut(s) 88, 122, 605, 756
AgsI TTSAA 1 cut(s) 190
AjnI CCWGG 1 cut(s) 562
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AloI GAACNNNNNNTCC 2 cut(s) 653, 685
AluBI AGCT 4 cut(s) 252, 370, 588, 632
AluI AGCT 4 cut(s) 252, 370, 588, 632
Alw21I GWGCWC 2 cut(s) 130, 590
Alw26I GTCTC 1 cut(s) 388
Alw44I GTGCAC 1 cut(s) 126
AlwI GGATC 4 cut(s) 561, 574, 741, 754
Ama87I CYCGRG 1 cut(s) 756
AoxI GGCC 7 cut(s) 78, 286, 291, 343, 361, 542, 731
ApaI GGGCCC 2 cut(s) 290, 546
ApaLI GTGCAC 1 cut(s) 126
ApeKI GCWGC 1 cut(s) 249
ApoI RAATTY 1 cut(s) 701
Asp700I GAANNNNTTC 1 cut(s) 668
AspLEI GCGC 1 cut(s) 73
AspS9I GGNCC 7 cut(s) 79, 108, 286, 287, 542, 543, 731
AsuC2I CCSGG 2 cut(s) 290, 405
AsuHPI GGTGA 5 cut(s) 185, 271, 370, 393, 471
AvaI CYCGRG 1 cut(s) 756
AvaII GGWCC 1 cut(s) 108
BaeGI GKGCMC 4 cut(s) 130, 290, 546, 640
BamHI GGATCC 2 cut(s) 566, 746
BanII GRGCYC 3 cut(s) 290, 546, 590
BbsI GAAGAC 1 cut(s) 23
Bbv12I GWGCWC 2 cut(s) 130, 590
BbvI GCAGC 1 cut(s) 261
BccI CCATC 3 cut(s) 16, 383, 526
BceAI ACGGC 2 cut(s) 93, 587
BciT130I CCWGG 1 cut(s) 564
BcnI CCSGG 2 cut(s) 290, 405
BcoDI GTCTC 1 cut(s) 388
BfaI CTAG 1 cut(s) 633
BglI GCCNNNNNGGC 2 cut(s) 360, 608
BisI GCNGC 6 cut(s) 45, 69, 250, 346, 581, 753
BlsI GCNGC 6 cut(s) 46, 70, 251, 347, 582, 754
BmcAI AGTACT 1 cut(s) 460
Bme1390I CCNGG 3 cut(s) 290, 405, 564
Bme18I GGWCC 1 cut(s) 108
BmeT110I CYCGRG 1 cut(s) 756
BmgT120I GGNCC 7 cut(s) 79, 108, 286, 287, 542, 543, 731
BmiI GGNNCC 6 cut(s) 288, 409, 543, 544, 568, 748
BmrFI CCNGG 3 cut(s) 290, 405, 564
BmsI GCATC 3 cut(s) 242, 398, 481
BpiI GAAGAC 1 cut(s) 23
BpuEI CTTGAG 1 cut(s) 731
BpuMI CCSGG 2 cut(s) 290, 405
BsaHI GRCGYC 1 cut(s) 149
BsaJI CCNNGG 3 cut(s) 282, 537, 750
BsaWI WCCGGW 1 cut(s) 152
BsaXI ACNNNNNCTCC 4 cut(s) 84, 114, 653, 683
Bsc4I CCNNNNNNNGG 4 cut(s) 88, 122, 605, 756
Bse118I RCCGGY 1 cut(s) 314
Bse1I ACTGG 2 cut(s) 355, 456
BseBI CCWGG 1 cut(s) 564
BseDI CCNNGG 3 cut(s) 282, 537, 750
BseGI GGATG 1 cut(s) 389
BseLI CCNNNNNNNGG 4 cut(s) 88, 122, 605, 756
BseMII CTCAG 1 cut(s) 399
BseNI ACTGG 2 cut(s) 355, 456
BseRI GAGGAG 1 cut(s) 728
BseSI GKGCMC 4 cut(s) 130, 290, 546, 640
BseX3I CGGCCG 1 cut(s) 343
BseXI GCAGC 1 cut(s) 261
BsgI GTGCAG 2 cut(s) 319, 405
Bsh1236I CGCG 2 cut(s) 197, 752
Bsh1285I CGRYCG 1 cut(s) 346
BshFI GGCC 7 cut(s) 80, 288, 293, 345, 363, 544, 733
BsiEI CGRYCG 1 cut(s) 346
BsiHKAI GWGCWC 2 cut(s) 130, 590
BsiHKCI CYCGRG 1 cut(s) 756
BsiSI CCGG 5 cut(s) 153, 290, 315, 360, 404
BslI CCNNNNNNNGG 4 cut(s) 88, 122, 605, 756
BsmAI GTCTC 1 cut(s) 388
BsmBI CGTCTC 1 cut(s) 388
BsnI GGCC 7 cut(s) 80, 288, 293, 345, 363, 544, 733
BsoBI CYCGRG 1 cut(s) 756
Bsp120I GGGCCC 2 cut(s) 286, 542
Bsp1286I GDGCHC 5 cut(s) 130, 290, 546, 590, 640
Bsp143I GATC 2 cut(s) 566, 746
Bsp68I TCGCGA 1 cut(s) 197
BspACI CCGC 7 cut(s) 44, 68, 346, 393, 581, 750, 752
BspANI GGCC 7 cut(s) 80, 288, 293, 345, 363, 544, 733
BspCNI CTCAG 1 cut(s) 398
BspFNI CGCG 2 cut(s) 197, 752
BspLI GGNNCC 6 cut(s) 288, 409, 543, 544, 568, 748
BspPI GGATC 4 cut(s) 561, 574, 741, 754
BspQI GCTCTTC 2 cut(s) 595, 622
BsrFI RCCGGY 1 cut(s) 314
BsrI ACTGG 2 cut(s) 355, 456
BssAI RCCGGY 1 cut(s) 314
BssECI CCNNGG 3 cut(s) 282, 537, 750
BssMI GATC 2 cut(s) 566, 746
BssNI GRCGYC 1 cut(s) 149
Bst2UI CCWGG 1 cut(s) 564
Bst4CI ACNGT 2 cut(s) 382, 574
Bst6I CTCTTC 2 cut(s) 595, 622
BstACI GRCGYC 1 cut(s) 149
BstC8I GCNNGC 3 cut(s) 316, 372, 553
BstDEI CTNAG 1 cut(s) 385
BstDSI CCRYGG 3 cut(s) 282, 537, 750
BstF5I GGATG 1 cut(s) 389
BstFNI CGCG 2 cut(s) 197, 752
BstHHI GCGC 1 cut(s) 73
BstKTI GATC 2 cut(s) 569, 749
BstMAI GTCTC 1 cut(s) 388
BstMBI GATC 2 cut(s) 566, 746
BstMCI CGRYCG 1 cut(s) 346
BstMWI GCNNNNNNNGC 7 cut(s) 77, 203, 342, 351, 360, 608, 761
BstNI CCWGG 1 cut(s) 564
BstSCI CCNGG 3 cut(s) 288, 403, 562
BstSLI GKGCMC 4 cut(s) 130, 290, 546, 640
BstUI CGCG 2 cut(s) 197, 752
BstV1I GCAGC 1 cut(s) 261
BstV2I GAAGAC 1 cut(s) 23
BstX2I RGATCY 2 cut(s) 566, 746
BstYI RGATCY 2 cut(s) 566, 746
BstZI CGGCCG 1 cut(s) 343
BsuRI GGCC 7 cut(s) 80, 288, 293, 345, 363, 544, 733
BtgI CCRYGG 3 cut(s) 282, 537, 750
BtsCI GGATG 1 cut(s) 389
BtsI GCAGTG 1 cut(s) 349
BtsIMutI CAGTG 2 cut(s) 349, 638
BtuMI TCGCGA 1 cut(s) 197
Cac8I GCNNGC 3 cut(s) 316, 372, 553
CfoI GCGC 1 cut(s) 73
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 7 cut(s) 79, 108, 286, 287, 542, 543, 731
Cfr42I CCGCGG 1 cut(s) 753
CseI GACGC 2 cut(s) 138, 212
Csp6I GTAC 3 cut(s) 224, 273, 459
CviAII CATG 4 cut(s) 83, 246, 397, 673
CviQI GTAC 3 cut(s) 224, 273, 459
DdeI CTNAG 1 cut(s) 385
DpnI GATC 2 cut(s) 568, 748
DpnII GATC 2 cut(s) 566, 746
DraI TTTAAA 1 cut(s) 706
EaeI YGGCCR 3 cut(s) 291, 343, 361
EagI CGGCCG 1 cut(s) 343
Eam1104I CTCTTC 2 cut(s) 595, 622
EarI CTCTTC 2 cut(s) 595, 622
EciI GGCGGA 1 cut(s) 382
Ecl136II GAGCTC 1 cut(s) 588
EclXI CGGCCG 1 cut(s) 343
Eco24I GRGCYC 3 cut(s) 290, 546, 590
Eco47I GGWCC 1 cut(s) 108
Eco52I CGGCCG 1 cut(s) 343
Eco53kI GAGCTC 1 cut(s) 588
Eco88I CYCGRG 1 cut(s) 756
EcoICRI GAGCTC 1 cut(s) 588
EcoO109I RGGNCCY 2 cut(s) 542, 731
EcoRII CCWGG 1 cut(s) 562
EcoT38I GRGCYC 3 cut(s) 290, 546, 590
Esp3I CGTCTC 1 cut(s) 388
FaeI CATG 4 cut(s) 86, 249, 400, 676
FaiI YATR 9 cut(s) 84, 113, 138, 167, 247, 398, 434, 674, 768
FatI CATG 4 cut(s) 82, 245, 396, 672
Fnu4HI GCNGC 6 cut(s) 45, 69, 250, 346, 581, 753
FokI GGATG 1 cut(s) 376
FriOI GRGCYC 3 cut(s) 290, 546, 590
Fsp4HI GCNGC 6 cut(s) 45, 69, 250, 346, 581, 753
FspBI CTAG 1 cut(s) 633
GlaI GCGC 1 cut(s) 72
GluI GCNGC 6 cut(s) 45, 69, 250, 346, 581, 753
HaeIII GGCC 7 cut(s) 80, 288, 293, 345, 363, 544, 733
HapII CCGG 5 cut(s) 153, 290, 315, 360, 404
HgaI GACGC 2 cut(s) 138, 212
HhaI GCGC 1 cut(s) 73
Hin1I GRCGYC 1 cut(s) 149
Hin1II CATG 4 cut(s) 86, 249, 400, 676
Hin6I GCGC 1 cut(s) 71
HinP1I GCGC 1 cut(s) 71
HinfI GANTC 1 cut(s) 242
HpaII CCGG 5 cut(s) 153, 290, 315, 360, 404
HphI GGTGA 5 cut(s) 185, 271, 370, 393, 471
Hpy166II GTNNAC 2 cut(s) 128, 528
Hpy188I TCNGA 3 cut(s) 159, 490, 746
Hpy188III TCNNGA 2 cut(s) 196, 758
Hpy8I GTNNAC 2 cut(s) 128, 528
Hpy99I CGWCG 2 cut(s) 215, 746
HpyAV CCTTC 5 cut(s) 196, 286, 426, 486, 722
HpyCH4III ACNGT 2 cut(s) 382, 574
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 5 cut(s) 128, 220, 249, 336, 422
HpyF10VI GCNNNNNNNGC 7 cut(s) 77, 203, 342, 351, 360, 608, 761
HpyF3I CTNAG 1 cut(s) 385
HpySE526I ACGT 1 cut(s) 213
Hsp92I GRCGYC 1 cut(s) 149
Hsp92II CATG 4 cut(s) 86, 249, 400, 676
HspAI GCGC 1 cut(s) 71
KroI GCCGGC 1 cut(s) 314
KroNI GCCGGC 1 cut(s) 316
KspI CCGCGG 1 cut(s) 753
Kzo9I GATC 2 cut(s) 566, 746
LguI GCTCTTC 2 cut(s) 595, 622
LmnI GCTCC 1 cut(s) 407
Lsp1109I GCAGC 1 cut(s) 261
LweI GCATC 3 cut(s) 242, 398, 481
MaeI CTAG 1 cut(s) 633
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 3 cut(s) 36, 139, 399
MalI GATC 2 cut(s) 568, 748
MboI GATC 2 cut(s) 566, 746
MboII GAAGA 7 cut(s) 25, 28, 582, 585, 639, 676, 691
MflI RGATCY 2 cut(s) 566, 746
MhlI GDGCHC 5 cut(s) 130, 290, 546, 590, 640
MluCI AATT 1 cut(s) 701
MlyI GAGTC 1 cut(s) 236
MmeI TCCRAC 2 cut(s) 639, 724
MnlI CCTC 8 cut(s) 86, 421, 447, 453, 513, 706, 711, 754
MroNI GCCGGC 1 cut(s) 314
MroXI GAANNNNTTC 1 cut(s) 668
MseI TTAA 1 cut(s) 705
MspA1I CMGCKG 1 cut(s) 752
MspI CCGG 5 cut(s) 153, 290, 315, 360, 404
MspR9I CCNGG 3 cut(s) 290, 405, 564
MvaI CCWGG 1 cut(s) 564
MvnI CGCG 2 cut(s) 197, 752
MwoI GCNNNNNNNGC 7 cut(s) 77, 203, 342, 351, 360, 608, 761
NaeI GCCGGC 1 cut(s) 316
NciI CCSGG 2 cut(s) 290, 405
NdeII GATC 2 cut(s) 566, 746
NgoMIV GCCGGC 1 cut(s) 314
NlaIII CATG 4 cut(s) 86, 249, 400, 676
NlaIV GGNNCC 6 cut(s) 288, 409, 543, 544, 568, 748
NmeAIII GCCGAG 1 cut(s) 609
NmuCI GTSAC 2 cut(s) 139, 399
NruI TCGCGA 1 cut(s) 197
PciSI GCTCTTC 2 cut(s) 595, 622
PcsI WCGNNNNNNNCGW 2 cut(s) 210, 378
PdiI GCCGGC 1 cut(s) 316
PdmI GAANNNNTTC 1 cut(s) 668
PflMI CCANNNNNTGG 2 cut(s) 88, 122
PkrI GCNGC 6 cut(s) 46, 70, 251, 347, 582, 754
PleI GAGTC 1 cut(s) 236
PpsI GAGTC 1 cut(s) 236
Psp124BI GAGCTC 1 cut(s) 590
Psp6I CCWGG 1 cut(s) 562
PspGI CCWGG 1 cut(s) 562
PspN4I GGNNCC 6 cut(s) 288, 409, 543, 544, 568, 748
PspOMI GGGCCC 2 cut(s) 286, 542
PspPI GGNCC 7 cut(s) 79, 108, 286, 287, 542, 543, 731
PsuI RGATCY 2 cut(s) 566, 746
RruI TCGCGA 1 cut(s) 197
RsaI GTAC 3 cut(s) 225, 274, 460
RsaNI GTAC 3 cut(s) 224, 273, 459
SacI GAGCTC 1 cut(s) 590
SacII CCGCGG 1 cut(s) 753
SapI GCTCTTC 2 cut(s) 595, 622
SaqAI TTAA 1 cut(s) 705
SatI GCNGC 6 cut(s) 45, 69, 250, 346, 581, 753
Sau3AI GATC 2 cut(s) 566, 746
Sau96I GGNCC 7 cut(s) 79, 108, 286, 287, 542, 543, 731
ScaI AGTACT 1 cut(s) 460
SchI GAGTC 1 cut(s) 236
ScrFI CCNGG 3 cut(s) 290, 405, 564
SduI GDGCHC 5 cut(s) 130, 290, 546, 590, 640
SfaNI GCATC 3 cut(s) 242, 398, 481
Sfr303I CCGCGG 1 cut(s) 753
SgrBI CCGCGG 1 cut(s) 753
SinI GGWCC 1 cut(s) 108
SmlI CTYRAG 1 cut(s) 710
SmoI CTYRAG 1 cut(s) 710
Sse9I AATT 1 cut(s) 701
SsiI CCGC 7 cut(s) 44, 68, 346, 393, 581, 750, 752
SspMI CTAG 1 cut(s) 633
SstI GAGCTC 1 cut(s) 590
StyD4I CCNGG 3 cut(s) 288, 403, 562
TaaI ACNGT 2 cut(s) 382, 574
TaiI ACGT 1 cut(s) 216
TaqI TCGA 2 cut(s) 240, 722
TasI AATT 1 cut(s) 701
TatI WGTACW 1 cut(s) 458
TauI GCSGC 5 cut(s) 47, 71, 348, 583, 755
Tru1I TTAA 1 cut(s) 705
Tru9I TTAA 1 cut(s) 705
TscAI CASTG 2 cut(s) 356, 645
TseFI GTSAC 2 cut(s) 139, 399
TseI GCWGC 1 cut(s) 249
Tsp45I GTSAC 2 cut(s) 139, 399
TspDTI ATGAA 2 cut(s) 154, 661
TspGWI ACGGA 1 cut(s) 513
TspRI CASTG 2 cut(s) 356, 645
Van91I CCANNNNNTGG 2 cut(s) 88, 122
VneI GTGCAC 1 cut(s) 126
VpaK11BI GGWCC 1 cut(s) 108
XapI RAATTY 1 cut(s) 701
XmnI GAANNNNTTC 1 cut(s) 668
XspI CTAG 1 cut(s) 633
ZrmI AGTACT 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.