FvH4_6g41960
ERF Family

Belongs to the AB hydrolase superfamily. Lipase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000628
Physical Location & Seq
Reverse (-)
32857987 .. 32859953
1967 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g41960.t1

Sequence Viewer

Length: 1224 bp
ATGGCGAACATTCTTACCTCCATTATTACAGTTGTTCTGTTCTGGGGATTGGCAGTTGGTACTAGAACAAAGCTATTCTCTGTCGAAGACAGCGTGGTTTCGGCAATGGCTCCATCCGGTAGCGATGGTATCTGTGCATCAATGGTTGTATCACAAGGCTACCCTTGTGAAGAACATACAGTAACAACACAAGATGGCTACATGCTCGGTATACAGAGAATTCCAGCTGGGAAGTCCAGGGAGACTTCCGGGAACAAGGTGCCGGTTCTTCTACAACATGGACTTCTTATGGATGGGATAACATGGCTGCTACTACCTCCAGACCAATCTTTGGCATTCATCTTGGCAGATAATGGGTATGAAGTGTGGATTGCCAATACCCGTGGAACCAAATATAGCCTTGGACATACATCACTCACCCCAGATGATCCGGCTTATTGGGAGTGGTCATGGGATGAACTGGTCGCTTATGACCTCCCGGCCGCATACCAATATGTGTATGATCAAGCAGGACAGAAGCTTCACTATGTTGGACATTCACTGGGAACTTTGATTGCTCTTGGTGCTTTTTCGGAAGACCAGCTAGTTAACATGTTGAGATCAGCTGTCTTACTTAGCCCAATTGCTCATGTTGGTCAGATAACCTCGCCCCTTGCGAAAACTGCTATTGAATACTTCATTGCTGAGGATTTGTACAAAGCAGGTTATAGAGAATTTGATCCAAAAGGACCATTGGTAGTCCAAGTTCTTAAGCAAATCTGTTCGAAACCAGGCGTTGACTGCACAGACTTGTTGACCTCTTTCACAGGCAAGAATTGCTGCTTAAATTCATCCATAGTGGACATTTTTCTAGACCACGAGCCTCAGGCAACTTCAATAAAAAACATGATCCACATCTCTCAGATGGTAAGAGACGGAAAAGTTGCGATGTTCGACTACAATGACAAGGAGGAGAATCAACAACACTACGGTCAGGACACTCCTCCAGAGTACAAAATGGCAAGCATTCCACATGACGTTCCTCTATTCCTCAGTTATGGAGGGGCAGATGCACTTTCTGATGTCCAAGATGTGCAGCTATTGCTCAACAGCCTCAAAGATCATGATGGAGATAAGCTTGTGGTTCAGTACAGAGATGATTACGCTCATGCAGATTTTGTAATGGGTGAAAATGCTAAGCAAGATGTGTATGATCCTCTCATGGGTTTCTTTAAACTTCAATGA

Protein Analysis

408

Amino Acids

44.96

Weight (kDa)

4.72

Isoelectric Point (pI)

31.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydro_lipase PF04083 47 - 104 4.5e-21 Partial alpha/beta-hydrolase lipase region
Abhydrolase_1 PF00561 88 - 219 3.1e-08 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000628)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14180 AT5G14180 AT5G14180 AT5G14180 AT5G14180
fragaria_vesca FvH4_6g41960 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g53200
malus_domestica MD09G1013100.v1.1 MD09G1115500.v1.1 MD09G1115600.v1.1 MD09G1115800.v1.1 MD17G1013800.v1.1 MD17G1107400.v1.1
prunus_persica Prupe.3G208900_v2.0.a1 Prupe.3G208900_v2.0.a1 Prupe.3G304100_v2.0.a1
pyrus_communis pycom09g04000 pycom09g04020 pycom09g04040 pycom111g01090 pycom111g01110 pycom17g01090 pycom17g10010
rosa_chinensis RchiOBHm_Chr2g0157861 RchiOBHm_Chr2g0157881 RchiOBHm_Chr2g0175241 RchiOBHm_Chr2g0175251
rosa_laevigata RLG00000021032 RLG00000021033 RLG00000022314 RLG00000022315
rosa_multiflora Rmu_co8368271.1_g000001 Rmu_co8519319.1_g000001 Rmu_co8519319.1_g000002 Rmu_sc0002441.1_g000007 Rmu_sc0002441.1_g000008 Rmu_sc0010083.1_g000002 Rmu_sc0015795.1_g000001 Rmu_sc0015795.1_g000002
rosa_roxburghii Rroxscaffold_1G00036740 Rroxscaffold_2G00077450 Rroxscaffold_2G00077460 Rroxscaffold_2G00091880 Rroxscaffold_2G00091890 Rroxscaffold_2G00127210
rosa_rugosa Rorug02G0464200 Rorug02G0464200 Rorug02G0582000 Rorug02G0582100 Rorug02G0582100 Rorug02G0582100 Rorug02G0582200 Rorug02G0582200 Rorug02G0582200
rosa_samantha Rh2AG529400 Rh2AG529500 Rh2AG662400 Rh2AG662500 Rh2BG544000 Rh2BG544100 Rh2BG673600 Rh2BG673700 Rh2CG513700 Rh2CG513800 Rh2CG636800 Rh2DG552400
rosa_wichuraiana Rw2G043800 Rw2G043810 Rw2G054330 Rw2G054340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 692
AccB1I GGYRCC 1 cut(s) 259
AccB7I CCANNNNNTGG 1 cut(s) 331
AccI GTMKAC 1 cut(s) 211
AciI CCGC 1 cut(s) 483
AclWI GGATC 4 cut(s) 422, 713, 883, 1187
AcoI YGGCCR 1 cut(s) 480
AcsI RAATTY 3 cut(s) 219, 713, 826
AfaI GTAC 4 cut(s) 61, 695, 992, 1130
AfiI CCNNNNNNNGG 2 cut(s) 331, 1202
AflII CTTAAG 1 cut(s) 749
AflIII ACRYGT 1 cut(s) 591
AgsI TTSAA 3 cut(s) 671, 876, 1220
AjnI CCWGG 2 cut(s) 236, 769
AluBI AGCT 7 cut(s) 73, 227, 520, 583, 605, 1078, 1117
AluI AGCT 7 cut(s) 73, 227, 520, 583, 605, 1078, 1117
Alw26I GTCTC 2 cut(s) 236, 906
AlwI GGATC 4 cut(s) 422, 713, 883, 1187
AoxI GGCC 1 cut(s) 480
ApeKI GCWGC 3 cut(s) 307, 819, 1075
ApoI RAATTY 3 cut(s) 219, 713, 826
AspS9I GGNCC 1 cut(s) 728
AsuC2I CCSGG 2 cut(s) 250, 479
AsuHPI GGTGA 2 cut(s) 409, 1178
AsuII TTCGAA 1 cut(s) 764
AvaII GGWCC 1 cut(s) 728
AxyI CCTNAGG 1 cut(s) 864
BanI GGYRCC 1 cut(s) 259
BauI CACGAG 1 cut(s) 857
BbsI GAAGAC 2 cut(s) 93, 582
BbvCI CCTCAGC 1 cut(s) 684
BbvI GCAGC 3 cut(s) 294, 806, 1087
BccI CCATC 6 cut(s) 119, 121, 188, 287, 898, 1100
BciT130I CCWGG 2 cut(s) 238, 771
BclI TGATCA 1 cut(s) 502
BcnI CCSGG 2 cut(s) 250, 479
BcoDI GTCTC 2 cut(s) 236, 906
BfaI CTAG 3 cut(s) 63, 584, 851
BfrI CTTAAG 1 cut(s) 749
BfuAI ACCTGC 1 cut(s) 692
BisI GCNGC 4 cut(s) 308, 483, 820, 1076
BlpI GCTNAGC 1 cut(s) 1176
BlsI GCNGC 4 cut(s) 309, 484, 821, 1077
Bme1390I CCNGG 4 cut(s) 238, 250, 479, 771
Bme18I GGWCC 1 cut(s) 728
BmgT120I GGNCC 1 cut(s) 728
BmiI GGNNCC 3 cut(s) 111, 261, 388
BmrFI CCNGG 4 cut(s) 238, 250, 479, 771
BmrI ACTGGG 1 cut(s) 551
BmsI GCATC 2 cut(s) 146, 1039
BmuI ACTGGG 1 cut(s) 551
BpiI GAAGAC 2 cut(s) 93, 582
BpmI CTGGAG 2 cut(s) 303, 969
Bpu10I CCTNAGC 1 cut(s) 684
Bpu1102I GCTNAGC 1 cut(s) 1176
Bpu14I TTCGAA 1 cut(s) 764
BpuMI CCSGG 2 cut(s) 250, 479
BsaBI GATNNNNATC 1 cut(s) 893
BsaJI CCNNGG 3 cut(s) 237, 382, 400
BsaWI WCCGGW 1 cut(s) 116
Bsc4I CCNNNNNNNGG 2 cut(s) 331, 1202
Bse118I RCCGGY 1 cut(s) 262
Bse1I ACTGG 2 cut(s) 465, 546
Bse21I CCTNAGG 1 cut(s) 864
Bse3DI GCAATG 2 cut(s) 111, 678
Bse8I GATNNNNATC 1 cut(s) 893
BseBI CCWGG 2 cut(s) 238, 771
BseDI CCNNGG 3 cut(s) 237, 382, 400
BseGI GGATG 4 cut(s) 113, 298, 460, 830
BseJI GATNNNNATC 1 cut(s) 893
BseLI CCNNNNNNNGG 2 cut(s) 331, 1202
BseMI GCAATG 2 cut(s) 111, 678
BseMII CTCAG 4 cut(s) 675, 878, 914, 1045
BseNI ACTGG 2 cut(s) 465, 546
BseRI GAGGAG 2 cut(s) 965, 972
BseX3I CGGCCG 1 cut(s) 480
BseXI GCAGC 3 cut(s) 294, 806, 1087
BseYI CCCAGC 1 cut(s) 227
BsgI GTGCAG 2 cut(s) 766, 1094
Bsh1285I CGRYCG 1 cut(s) 483
BshFI GGCC 1 cut(s) 482
BshNI GGYRCC 1 cut(s) 259
BsiEI CGRYCG 1 cut(s) 483
BsiSI CCGG 5 cut(s) 117, 249, 263, 431, 479
BslI CCNNNNNNNGG 2 cut(s) 331, 1202
BsmAI GTCTC 2 cut(s) 236, 906
BsmBI CGTCTC 1 cut(s) 906
BsmI GAATGC 2 cut(s) 335, 1005
BsnI GGCC 1 cut(s) 482
Bsp119I TTCGAA 1 cut(s) 764
Bsp1407I TGTACA 1 cut(s) 693
Bsp143I GATC 7 cut(s) 427, 502, 599, 718, 888, 1099, 1192
Bsp1720I GCTNAGC 1 cut(s) 1176
BspACI CCGC 1 cut(s) 483
BspANI GGCC 1 cut(s) 482
BspCNI CTCAG 4 cut(s) 676, 877, 913, 1044
BspHI TCATGA 1 cut(s) 1102
BspLI GGNNCC 3 cut(s) 111, 261, 388
BspMI ACCTGC 1 cut(s) 692
BspPI GGATC 4 cut(s) 422, 713, 883, 1187
BspT104I TTCGAA 1 cut(s) 764
BspT107I GGYRCC 1 cut(s) 259
BspTI CTTAAG 1 cut(s) 749
BsrDI GCAATG 2 cut(s) 111, 678
BsrFI RCCGGY 1 cut(s) 262
BsrGI TGTACA 1 cut(s) 693
BsrI ACTGG 2 cut(s) 465, 546
BssAI RCCGGY 1 cut(s) 262
BssECI CCNNGG 3 cut(s) 237, 382, 400
BssMI GATC 7 cut(s) 427, 502, 599, 718, 888, 1099, 1192
BssNAI GTATAC 1 cut(s) 212
BssSI CACGAG 1 cut(s) 857
BssT1I CCWWGG 1 cut(s) 400
Bst1107I GTATAC 1 cut(s) 212
Bst2BI CACGAG 1 cut(s) 857
Bst2UI CCWGG 2 cut(s) 238, 771
Bst4CI ACNGT 3 cut(s) 31, 181, 971
BstAFI CTTAAG 1 cut(s) 749
BstAPI GCANNNNNTGC 2 cut(s) 816, 1081
BstAUI TGTACA 1 cut(s) 693
BstBI TTCGAA 1 cut(s) 764
BstC8I GCNNGC 1 cut(s) 1003
BstDEI CTNAG 6 cut(s) 614, 684, 864, 900, 1031, 1176
BstDSI CCRYGG 1 cut(s) 382
BstF5I GGATG 4 cut(s) 113, 298, 460, 830
BstKTI GATC 7 cut(s) 430, 505, 602, 721, 891, 1102, 1195
BstMAI GTCTC 2 cut(s) 236, 906
BstMBI GATC 7 cut(s) 427, 502, 599, 718, 888, 1099, 1192
BstMCI CGRYCG 1 cut(s) 483
BstMWI GCNNNNNNNGC 5 cut(s) 563, 662, 780, 816, 1081
BstNI CCWGG 2 cut(s) 238, 771
BstNSI RCATGY 2 cut(s) 205, 595
BstSCI CCNGG 4 cut(s) 236, 248, 477, 769
BstV1I GCAGC 3 cut(s) 294, 806, 1087
BstV2I GAAGAC 2 cut(s) 93, 582
BstZ17I GTATAC 1 cut(s) 212
BstZI CGGCCG 1 cut(s) 480
Bsu36I CCTNAGG 1 cut(s) 864
BsuRI GGCC 1 cut(s) 482
BtgI CCRYGG 1 cut(s) 382
BtgZI GCGATG 2 cut(s) 138, 941
BtsCI GGATG 4 cut(s) 113, 298, 460, 830
BtsIMutI CAGTG 1 cut(s) 539
BveI ACCTGC 1 cut(s) 692
Cac8I GCNNGC 1 cut(s) 1003
CciI TCATGA 1 cut(s) 1102
Cfr10I RCCGGY 1 cut(s) 262
Cfr13I GGNCC 1 cut(s) 728
Csp6I GTAC 4 cut(s) 60, 694, 991, 1129
CspCI CAANNNNNGTGG 2 cut(s) 364, 399
CviQI GTAC 4 cut(s) 60, 694, 991, 1129
DdeI CTNAG 6 cut(s) 614, 684, 864, 900, 1031, 1176
DpnI GATC 7 cut(s) 429, 504, 601, 720, 890, 1101, 1194
DpnII GATC 7 cut(s) 427, 502, 599, 718, 888, 1099, 1192
DraI TTTAAA 1 cut(s) 1213
EaeI YGGCCR 1 cut(s) 480
EagI CGGCCG 1 cut(s) 480
EclXI CGGCCG 1 cut(s) 480
Eco130I CCWWGG 1 cut(s) 400
Eco47I GGWCC 1 cut(s) 728
Eco52I CGGCCG 1 cut(s) 480
Eco81I CCTNAGG 1 cut(s) 864
EcoRI GAATTC 1 cut(s) 219
EcoRII CCWGG 2 cut(s) 236, 769
EcoT14I CCWWGG 1 cut(s) 400
ErhI CCWWGG 1 cut(s) 400
Esp3I CGTCTC 1 cut(s) 906
FbaI TGATCA 1 cut(s) 502
FblI GTMKAC 1 cut(s) 211
Fnu4HI GCNGC 4 cut(s) 308, 483, 820, 1076
FokI GGATG 4 cut(s) 100, 305, 467, 817
Fsp4HI GCNGC 4 cut(s) 308, 483, 820, 1076
FspBI CTAG 3 cut(s) 63, 584, 851
GluI GCNGC 4 cut(s) 308, 483, 820, 1076
GsaI CCCAGC 1 cut(s) 231
GsuI CTGGAG 2 cut(s) 303, 969
HaeIII GGCC 1 cut(s) 482
HapII CCGG 5 cut(s) 117, 249, 263, 431, 479
HincII GTYRAC 3 cut(s) 589, 778, 795
HindII GTYRAC 3 cut(s) 589, 778, 795
HindIII AAGCTT 2 cut(s) 518, 1115
HinfI GANTC 1 cut(s) 955
HpaI GTTAAC 1 cut(s) 589
HpaII CCGG 5 cut(s) 117, 249, 263, 431, 479
HphI GGTGA 2 cut(s) 409, 1178
Hpy166II GTNNAC 5 cut(s) 212, 589, 778, 795, 841
Hpy188I TCNGA 4 cut(s) 574, 639, 903, 1060
Hpy188III TCNNGA 5 cut(s) 320, 851, 974, 986, 1103
Hpy8I GTNNAC 5 cut(s) 212, 589, 778, 795, 841
HpyCH4III ACNGT 3 cut(s) 31, 181, 971
HpyCH4IV ACGT 1 cut(s) 1017
HpyCH4V TGCA 5 cut(s) 137, 783, 1052, 1075, 1151
HpyF10VI GCNNNNNNNGC 5 cut(s) 563, 662, 780, 816, 1081
HpyF3I CTNAG 6 cut(s) 614, 684, 864, 900, 1031, 1176
HpySE526I ACGT 1 cut(s) 1017
Ksp22I TGATCA 1 cut(s) 502
KspAI GTTAAC 1 cut(s) 589
Kzo9I GATC 7 cut(s) 427, 502, 599, 718, 888, 1099, 1192
LmnI GCTCC 1 cut(s) 115
Lsp1109I GCAGC 3 cut(s) 294, 806, 1087
LweI GCATC 2 cut(s) 146, 1039
MaeI CTAG 3 cut(s) 63, 584, 851
MaeII ACGT 1 cut(s) 1017
MaeIII GTNAC 1 cut(s) 181
MalI GATC 7 cut(s) 429, 504, 601, 720, 890, 1101, 1194
MboI GATC 7 cut(s) 427, 502, 599, 718, 888, 1099, 1192
MboII GAAGA 4 cut(s) 98, 182, 260, 587
MfeI CAATTG 1 cut(s) 621
MluCI AATT 5 cut(s) 219, 621, 713, 814, 826
MmeI TCCRAC 1 cut(s) 511
MseI TTAA 4 cut(s) 588, 750, 824, 1212
MspA1I CMGCKG 2 cut(s) 227, 605
MspCI CTTAAG 1 cut(s) 749
MspI CCGG 5 cut(s) 117, 249, 263, 431, 479
MspR9I CCNGG 4 cut(s) 238, 250, 479, 771
MunI CAATTG 1 cut(s) 621
Mva1269I GAATGC 2 cut(s) 335, 1005
MvaI CCWGG 2 cut(s) 238, 771
MwoI GCNNNNNNNGC 5 cut(s) 563, 662, 780, 816, 1081
NciI CCSGG 2 cut(s) 250, 479
NdeII GATC 7 cut(s) 427, 502, 599, 718, 888, 1099, 1192
NlaIV GGNNCC 3 cut(s) 111, 261, 388
NspI RCATGY 2 cut(s) 205, 595
NspV TTCGAA 1 cut(s) 764
PagI TCATGA 1 cut(s) 1102
PciI ACATGT 1 cut(s) 591
PcsI WCGNNNNNNNCGW 2 cut(s) 90, 653
PctI GAATGC 2 cut(s) 335, 1005
PfeI GAWTC 1 cut(s) 955
PflMI CCANNNNNTGG 1 cut(s) 331
PfoI TCCNGGA 1 cut(s) 248
PkrI GCNGC 4 cut(s) 309, 484, 821, 1077
PscI ACATGT 1 cut(s) 591
Psp6I CCWGG 2 cut(s) 236, 769
PspFI CCCAGC 1 cut(s) 227
PspGI CCWGG 2 cut(s) 236, 769
PspN4I GGNNCC 3 cut(s) 111, 261, 388
PspPI GGNCC 1 cut(s) 728
PsrI GAACNNNNNNTAC 2 cut(s) 729, 761
PvuII CAGCTG 2 cut(s) 227, 605
RsaI GTAC 4 cut(s) 61, 695, 992, 1130
RsaNI GTAC 4 cut(s) 60, 694, 991, 1129
SaqAI TTAA 4 cut(s) 588, 750, 824, 1212
SatI GCNGC 4 cut(s) 308, 483, 820, 1076
Sau3AI GATC 7 cut(s) 427, 502, 599, 718, 888, 1099, 1192
Sau96I GGNCC 1 cut(s) 728
ScrFI CCNGG 4 cut(s) 238, 250, 479, 771
SfaNI GCATC 2 cut(s) 146, 1039
SfuI TTCGAA 1 cut(s) 764
SinI GGWCC 1 cut(s) 728
SmlI CTYRAG 1 cut(s) 749
SmoI CTYRAG 1 cut(s) 749
Sse9I AATT 5 cut(s) 219, 621, 713, 814, 826
SsiI CCGC 1 cut(s) 483
SspMI CTAG 3 cut(s) 63, 584, 851
StyD4I CCNGG 4 cut(s) 236, 248, 477, 769
StyI CCWWGG 1 cut(s) 400
TaaI ACNGT 3 cut(s) 31, 181, 971
TaiI ACGT 1 cut(s) 1020
TaqI TCGA 3 cut(s) 84, 764, 933
TasI AATT 5 cut(s) 219, 621, 713, 814, 826
TatI WGTACW 3 cut(s) 693, 990, 1128
TauI GCSGC 1 cut(s) 485
TfiI GAWTC 1 cut(s) 955
Tru1I TTAA 4 cut(s) 588, 750, 824, 1212
Tru9I TTAA 4 cut(s) 588, 750, 824, 1212
TscAI CASTG 1 cut(s) 546
TseI GCWGC 3 cut(s) 307, 819, 1075
TspDTI ATGAA 5 cut(s) 328, 375, 471, 667, 819
TspGWI ACGGA 1 cut(s) 930
TspRI CASTG 1 cut(s) 546
Van91I CCANNNNNTGG 1 cut(s) 331
Vha464I CTTAAG 1 cut(s) 749
VpaK11BI GGWCC 1 cut(s) 728
XapI RAATTY 3 cut(s) 219, 713, 826
XbaI TCTAGA 1 cut(s) 850
XceI RCATGY 2 cut(s) 205, 595
XmiI GTMKAC 1 cut(s) 211
XspI CTAG 3 cut(s) 63, 584, 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.