Rroxscaffold_2G00077450
ERF Family

Belongs to the AB hydrolase superfamily. Lipase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000628
Physical Location & Seq
Reverse (-)
986397 .. 990725
4329 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00077450.1

Sequence Viewer

Length: 1623 bp
ATGTCTCTGCGCAGTTTAGCTTTTTGCATGGTGATGGTTTTGGTTTTGGTGGTTGCACTTGCGCCTGGCGCGGTGGAGGGCTCGAGTCGGAAGCTTTTGGCTCAGGCAAATGCGGCTGTGGCTGCACAGGCGTACGGTATATGCAACGCCTTGGTAACTGTGCATGGTTACCAATGCCAAGAGATAACTGTGACTACCGATGATGGTTACATACTGAGCGTGCAGAGAATTGGTGGTGGTGGCACCGGGAAACCACCGGTGATAATTCAGCATGGTGTTCTTGTGGATGCAGCAGTCTGGCTGATGAACTCTCCGGATAAAAATCTGCCGTTGATTCTAGCTGATAGTGGATTTGATGTGTGGATGGCTAACACCCGAGGGACCAGATTTAGCCGACGTCATACCTCCATGGACCCCAGCGATCCGGGGACTTTGTTAGCATTGGCTTCGCTTTCAGAAGGGAAGCTAGTGGATCAGCTGAAATCAGTAGCTTTGCTAACTCCCATTGCTTACCTGACTCACATGAGTACACCAATATGTGCGGTTGGGGCCAGACTCTTTGCTGGCGAGATCATAACAGCATTTCGTTTTGCAGAATTCGATCCAAAAGGAGAGCCCGTGGCTGAATTCTTAGATCATCTATGTGCCACTCCAGGAGTTCCATGTTACGACATATTCTCAGTACTCACCGGCAAAAATTGTTGTCTCAATGCTTCCACTGTTGATCTCTTCTTGGATCATCAGCCTCAGTCAACATCAACCAAGAACCTGGTGCACCTGTCTCAAGTTGTACGAAATGGCATTTTGACAAAATACGACCACGGGAGATCGTACACTAATTATATGCACTATGGCTCATTTTTCGCTCCGCATTACGATCTTTCTAAAATTCCCAACAACCTGCCCATGTTCATCACCTACGGCGGCCAAGATGCGCTCTCCGATGAACTCGACGTGTTGCATTTACTTGATCATTTGAAGTCTCACGACGCGGGAAAGCTTGTGGTTGATTTCATCCCCGAGTATGCCCATTTCGATTTTGTCACGGGGATCAATGCCAAGGACCTTGTGTATAATAAAATTCCACACAAATCAGATGAACAATCACCAAAAAGTTTCCGAGCTTTGGTTGCAAAGAAAGGCTTGGCAGCACAACATGTAGTTGGGGGCAGCCAAGGTTGGGAAGAATCCACAGACTTCAACTCCTGGGCATCTGCTCAGAAATTCAAGGTTGGCGATCAACTTGTTTTCAAGTACACTTCGGGTTTGCACAGTGTCGTTGAACTACCTAACGAGAGTGCCTTCAAGAACTGTGACCTTGGGAGTGCACTGGACTCGAAGAAGGGAGGAAACGATGTGGTGAAGTTGAACAAGGCCGGTACAAGATATTTTGCTTGTGGTACTTTAGGCCACTGTGACCAAGGCATGAAGGTGAAGATCACTACTGCAGGAAGCACTGCAGCTTCTTCTCCGGCATCATCATCCTCGAACGATGATGGTTCTTCTTCAGATGCTTCTACCACTACTTCAACTACTTCTGCAGCTTCTGCCAACTTCAATTCTTTCGCTTTGTTCTTTGCGATGACTGCAGTATCGGCACTCGTTCTGGTTTATGTGTTTTGA

Protein Analysis

540

Amino Acids

57.77

Weight (kDa)

6.21

Isoelectric Point (pI)

31.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydro_lipase PF04083 51 - 103 1.2e-15 Partial alpha/beta-hydrolase lipase region
Cu_bind_like PF02298 394 - 476 6e-22 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000628)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14180 AT5G14180 AT5G14180 AT5G14180 AT5G14180
fragaria_vesca FvH4_6g41960 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g53200
malus_domestica MD09G1013100.v1.1 MD09G1115500.v1.1 MD09G1115600.v1.1 MD09G1115800.v1.1 MD17G1013800.v1.1 MD17G1107400.v1.1
prunus_persica Prupe.3G208900_v2.0.a1 Prupe.3G208900_v2.0.a1 Prupe.3G304100_v2.0.a1
pyrus_communis pycom09g04000 pycom09g04020 pycom09g04040 pycom111g01090 pycom111g01110 pycom17g01090 pycom17g10010
rosa_chinensis RchiOBHm_Chr2g0157861 RchiOBHm_Chr2g0157881 RchiOBHm_Chr2g0175241 RchiOBHm_Chr2g0175251
rosa_laevigata RLG00000021032 RLG00000021033 RLG00000022314 RLG00000022315
rosa_multiflora Rmu_co8368271.1_g000001 Rmu_co8519319.1_g000001 Rmu_co8519319.1_g000002 Rmu_sc0002441.1_g000007 Rmu_sc0002441.1_g000008 Rmu_sc0010083.1_g000002 Rmu_sc0015795.1_g000001 Rmu_sc0015795.1_g000002
rosa_roxburghii Rroxscaffold_1G00036740 Rroxscaffold_2G00077450 Rroxscaffold_2G00077460 Rroxscaffold_2G00091880 Rroxscaffold_2G00091890 Rroxscaffold_2G00127210
rosa_rugosa Rorug02G0464200 Rorug02G0464200 Rorug02G0582000 Rorug02G0582100 Rorug02G0582100 Rorug02G0582100 Rorug02G0582200 Rorug02G0582200 Rorug02G0582200
rosa_samantha Rh2AG529400 Rh2AG529500 Rh2AG662400 Rh2AG662500 Rh2BG544000 Rh2BG544100 Rh2BG673600 Rh2BG673700 Rh2CG513700 Rh2CG513800 Rh2CG636800 Rh2DG552400
rosa_wichuraiana Rw2G043800 Rw2G043810 Rw2G054330 Rw2G054340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 400
Acc16I TGCGCA 1 cut(s) 11
Acc36I ACCTGC 1 cut(s) 909
AccB1I GGYRCC 1 cut(s) 242
AccII CGCG 2 cut(s) 71, 992
AccIII TCCGGA 1 cut(s) 313
AciI CCGC 6 cut(s) 71, 113, 542, 869, 924, 992
AclWI GGATC 5 cut(s) 416, 480, 596, 744, 1058
AcoI YGGCCR 1 cut(s) 925
AcsI RAATTY 5 cut(s) 596, 626, 888, 1080, 1223
AcuI CTGAAG 1 cut(s) 1491
AcyI GRCGYC 1 cut(s) 397
AfaI GTAC 8 cut(s) 134, 529, 684, 792, 833, 1256, 1381, 1402
AfiI CCNNNNNNNGG 2 cut(s) 1126, 1180
AflIII ACRYGT 2 cut(s) 954, 1156
AgeI ACCGGT 1 cut(s) 256
AgsI TTSAA 9 cut(s) 979, 1201, 1228, 1252, 1283, 1306, 1369, 1530, 1558
AjiI CACGTC 1 cut(s) 955
AjnI CCWGG 4 cut(s) 64, 652, 768, 1205
Alw21I GWGCWC 2 cut(s) 777, 1330
Alw26I GTCTC 4 cut(s) 9, 710, 786, 987
Alw44I GTGCAC 2 cut(s) 773, 1326
AlwI GGATC 5 cut(s) 416, 480, 596, 744, 1058
AlwNI CAGNNNCTG 1 cut(s) 1547
Ama87I CYCGRG 3 cut(s) 82, 375, 1019
Aor13HI TCCGGA 1 cut(s) 313
AoxI GGCC 4 cut(s) 549, 925, 1374, 1408
ApaLI GTGCAC 2 cut(s) 773, 1326
ApeKI GCWGC 6 cut(s) 122, 290, 1148, 1170, 1460, 1541
ApoI RAATTY 5 cut(s) 596, 626, 888, 1080, 1223
AsiGI ACCGGT 1 cut(s) 256
AspLEI GCGC 4 cut(s) 12, 64, 71, 937
AspS9I GGNCC 4 cut(s) 381, 412, 549, 1063
AsuC2I CCSGG 2 cut(s) 247, 426
AsuHPI GGTGA 7 cut(s) 43, 271, 679, 907, 1098, 1372, 1444
AvaI CYCGRG 3 cut(s) 82, 375, 1019
AvaII GGWCC 3 cut(s) 381, 412, 1063
BaeGI GKGCMC 2 cut(s) 777, 1330
BaeI ACNNNNGTAYC 2 cut(s) 1575, 1608
BanI GGYRCC 1 cut(s) 242
BanII GRGCYC 2 cut(s) 83, 618
Bbv12I GWGCWC 2 cut(s) 777, 1330
BbvI GCAGC 6 cut(s) 109, 302, 1160, 1182, 1472, 1553
BccI CCATC 4 cut(s) 28, 197, 358, 1490
BceAI ACGGC 2 cut(s) 313, 937
BcgI CGANNNNNNTGC 4 cut(s) 429, 463, 1317, 1351
BciT130I CCWGG 4 cut(s) 66, 654, 770, 1207
BclI TGATCA 1 cut(s) 970
BcnI CCSGG 2 cut(s) 247, 426
BcoDI GTCTC 4 cut(s) 9, 710, 786, 987
BfaI CTAG 2 cut(s) 338, 467
BfmI CTRYAG 4 cut(s) 1446, 1458, 1539, 1587
BfuAI ACCTGC 1 cut(s) 909
BisI GCNGC 8 cut(s) 114, 123, 291, 925, 1149, 1171, 1461, 1542
BlsI GCNGC 8 cut(s) 115, 124, 292, 926, 1150, 1172, 1462, 1543
BmcAI AGTACT 1 cut(s) 684
Bme1390I CCNGG 6 cut(s) 66, 247, 426, 654, 770, 1207
Bme18I GGWCC 3 cut(s) 381, 412, 1063
BmeT110I CYCGRG 3 cut(s) 82, 375, 1019
BmgBI CACGTC 1 cut(s) 955
BmgT120I GGNCC 4 cut(s) 381, 412, 549, 1063
BmiI GGNNCC 4 cut(s) 244, 382, 414, 550
BmrFI CCNGG 6 cut(s) 66, 247, 426, 654, 770, 1207
BmsI GCATC 5 cut(s) 277, 922, 1220, 1484, 1501
BpmI CTGGAG 1 cut(s) 636
Bpu10I CCTNAGC 1 cut(s) 102
BpuEI CTTGAG 1 cut(s) 768
BpuMI CCSGG 2 cut(s) 247, 426
BsaBI GATNNNNATC 1 cut(s) 321
BsaHI GRCGYC 1 cut(s) 397
BsaWI WCCGGW 2 cut(s) 256, 313
BsaXI ACNNNNNCTCC 4 cut(s) 603, 633, 1187, 1217
Bsc4I CCNNNNNNNGG 2 cut(s) 1126, 1180
Bse118I RCCGGY 3 cut(s) 256, 689, 1376
Bse1I ACTGG 1 cut(s) 1335
Bse3DI GCAATG 1 cut(s) 504
Bse8I GATNNNNATC 1 cut(s) 321
BseAI TCCGGA 1 cut(s) 313
BseBI CCWGG 4 cut(s) 66, 654, 770, 1207
BseGI GGATG 4 cut(s) 292, 369, 1014, 1481
BseJI GATNNNNATC 1 cut(s) 321
BseLI CCNNNNNNNGG 2 cut(s) 1126, 1180
BseMI GCAATG 1 cut(s) 504
BseMII CTCAG 5 cut(s) 116, 206, 693, 761, 1232
BseNI ACTGG 1 cut(s) 1335
BseSI GKGCMC 2 cut(s) 777, 1330
BseXI GCAGC 6 cut(s) 109, 302, 1160, 1182, 1472, 1553
BseYI CCCAGC 1 cut(s) 416
BsgI GTGCAG 2 cut(s) 108, 242
Bsh1236I CGCG 2 cut(s) 71, 992
BshFI GGCC 4 cut(s) 551, 927, 1376, 1410
BshNI GGYRCC 1 cut(s) 242
BshTI ACCGGT 1 cut(s) 256
BsiHKAI GWGCWC 2 cut(s) 777, 1330
BsiHKCI CYCGRG 3 cut(s) 82, 375, 1019
BsiSI CCGG 7 cut(s) 246, 257, 314, 425, 690, 1377, 1472
BsiWI CGTACG 1 cut(s) 132
BslFI GGGAC 2 cut(s) 394, 442
BslI CCNNNNNNNGG 2 cut(s) 1126, 1180
BsmAI GTCTC 4 cut(s) 9, 710, 786, 987
BsmFI GGGAC 2 cut(s) 394, 442
BsnI GGCC 4 cut(s) 551, 927, 1376, 1410
BsoBI CYCGRG 3 cut(s) 82, 375, 1019
Bsp1286I GDGCHC 4 cut(s) 83, 618, 777, 1330
Bsp13I TCCGGA 1 cut(s) 313
Bsp19I CCATGG 1 cut(s) 408
BspACI CCGC 6 cut(s) 71, 113, 542, 869, 924, 992
BspANI GGCC 4 cut(s) 551, 927, 1376, 1410
BspCNI CTCAG 5 cut(s) 115, 207, 692, 760, 1231
BspEI TCCGGA 1 cut(s) 313
BspFNI CGCG 2 cut(s) 71, 992
BspLI GGNNCC 4 cut(s) 244, 382, 414, 550
BspMAI CTGCAG 4 cut(s) 1450, 1462, 1543, 1591
BspMI ACCTGC 1 cut(s) 909
BspPI GGATC 5 cut(s) 416, 480, 596, 744, 1058
BspT107I GGYRCC 1 cut(s) 242
BsrDI GCAATG 1 cut(s) 504
BsrFI RCCGGY 3 cut(s) 256, 689, 1376
BsrI ACTGG 1 cut(s) 1335
BssAI RCCGGY 3 cut(s) 256, 689, 1376
BssNI GRCGYC 1 cut(s) 397
BssT1I CCWWGG 6 cut(s) 150, 408, 1059, 1174, 1318, 1420
Bst2UI CCWGG 4 cut(s) 66, 654, 770, 1207
Bst4CI ACNGT 7 cut(s) 137, 160, 190, 721, 1274, 1313, 1415
Bst6I CTCTTC 1 cut(s) 734
BstACI GRCGYC 1 cut(s) 397
BstAPI GCANNNNNTGC 1 cut(s) 1547
BstC8I GCNNGC 2 cut(s) 221, 565
BstDEI CTNAG 6 cut(s) 102, 215, 631, 679, 747, 1218
BstDSI CCRYGG 3 cut(s) 408, 618, 820
BstEII GGTNACC 1 cut(s) 167
BstF5I GGATG 4 cut(s) 292, 369, 1014, 1481
BstFNI CGCG 2 cut(s) 71, 992
BstHHI GCGC 4 cut(s) 12, 64, 71, 937
BstMAI GTCTC 4 cut(s) 9, 710, 786, 987
BstNI CCWGG 4 cut(s) 66, 654, 770, 1207
BstNSI RCATGY 1 cut(s) 1160
BstPI GGTNACC 1 cut(s) 167
BstSCI CCNGG 6 cut(s) 64, 245, 424, 652, 768, 1205
BstSFI CTRYAG 4 cut(s) 1446, 1458, 1539, 1587
BstSLI GKGCMC 2 cut(s) 777, 1330
BstUI CGCG 2 cut(s) 71, 992
BstV1I GCAGC 6 cut(s) 109, 302, 1160, 1182, 1472, 1553
BstXI CCANNNNNNTGG 1 cut(s) 769
BsuRI GGCC 4 cut(s) 551, 927, 1376, 1410
BtgI CCRYGG 3 cut(s) 408, 618, 820
BtgZI GCGATG 1 cut(s) 1595
BtrI CACGTC 1 cut(s) 955
BtsCI GGATG 4 cut(s) 292, 369, 1014, 1481
BtsI GCAGTG 1 cut(s) 1455
BtsIMutI CAGTG 5 cut(s) 717, 1279, 1328, 1411, 1455
BveI ACCTGC 1 cut(s) 909
Cac8I GCNNGC 2 cut(s) 221, 565
CaiI CAGNNNCTG 1 cut(s) 1547
CfoI GCGC 4 cut(s) 12, 64, 71, 937
Cfr10I RCCGGY 3 cut(s) 256, 689, 1376
Cfr13I GGNCC 4 cut(s) 381, 412, 549, 1063
CseI GACGC 1 cut(s) 998
CsiI ACCWGGT 1 cut(s) 768
Csp6I GTAC 8 cut(s) 133, 528, 683, 791, 832, 1255, 1380, 1401
CspAI ACCGGT 1 cut(s) 256
CviAII CATG 9 cut(s) 28, 164, 272, 409, 523, 663, 907, 1157, 1426
CviQI GTAC 8 cut(s) 133, 528, 683, 791, 832, 1255, 1380, 1401
DdeI CTNAG 6 cut(s) 102, 215, 631, 679, 747, 1218
EaeI YGGCCR 1 cut(s) 925
Eam1104I CTCTTC 1 cut(s) 734
EarI CTCTTC 1 cut(s) 734
Eco130I CCWWGG 6 cut(s) 150, 408, 1059, 1174, 1318, 1420
Eco24I GRGCYC 2 cut(s) 83, 618
Eco47I GGWCC 3 cut(s) 381, 412, 1063
Eco57I CTGAAG 1 cut(s) 1491
Eco88I CYCGRG 3 cut(s) 82, 375, 1019
Eco91I GGTNACC 1 cut(s) 167
EcoO109I RGGNCCY 1 cut(s) 1063
EcoO65I GGTNACC 1 cut(s) 167
EcoRI GAATTC 2 cut(s) 596, 626
EcoRII CCWGG 4 cut(s) 64, 652, 768, 1205
EcoT14I CCWWGG 6 cut(s) 150, 408, 1059, 1174, 1318, 1420
EcoT38I GRGCYC 2 cut(s) 83, 618
ErhI CCWWGG 6 cut(s) 150, 408, 1059, 1174, 1318, 1420
FaeI CATG 9 cut(s) 31, 167, 275, 412, 526, 666, 910, 1160, 1429
FalI AAGNNNNNCTT 2 cut(s) 1127, 1159
FaqI GGGAC 2 cut(s) 394, 442
FatI CATG 9 cut(s) 27, 163, 271, 408, 522, 662, 906, 1156, 1425
FauI CCCGC 1 cut(s) 985
FbaI TGATCA 1 cut(s) 970
Fnu4HI GCNGC 8 cut(s) 114, 123, 291, 925, 1149, 1171, 1461, 1542
FokI GGATG 4 cut(s) 299, 376, 1001, 1468
FriOI GRGCYC 2 cut(s) 83, 618
Fsp4HI GCNGC 8 cut(s) 114, 123, 291, 925, 1149, 1171, 1461, 1542
FspBI CTAG 2 cut(s) 338, 467
FspI TGCGCA 1 cut(s) 11
GlaI GCGC 4 cut(s) 11, 63, 70, 936
GluI GCNGC 8 cut(s) 114, 123, 291, 925, 1149, 1171, 1461, 1542
GsaI CCCAGC 1 cut(s) 420
GsuI CTGGAG 1 cut(s) 636
HaeIII GGCC 4 cut(s) 551, 927, 1376, 1410
HapII CCGG 7 cut(s) 246, 257, 314, 425, 690, 1377, 1472
HgaI GACGC 1 cut(s) 998
HhaI GCGC 4 cut(s) 12, 64, 71, 937
Hin1I GRCGYC 1 cut(s) 397
Hin1II CATG 9 cut(s) 31, 167, 275, 412, 526, 666, 910, 1160, 1429
Hin6I GCGC 4 cut(s) 10, 62, 69, 935
HinP1I GCGC 4 cut(s) 10, 62, 69, 935
HincII GTYRAC 1 cut(s) 753
HindII GTYRAC 1 cut(s) 753
HindIII AAGCTT 2 cut(s) 92, 998
HinfI GANTC 6 cut(s) 85, 334, 517, 555, 1187, 1334
HpaII CCGG 7 cut(s) 246, 257, 314, 425, 690, 1377, 1472
HphI GGTGA 7 cut(s) 43, 271, 679, 907, 1098, 1372, 1444
Hpy166II GTNNAC 6 cut(s) 530, 753, 775, 834, 1257, 1328
Hpy188I TCNGA 7 cut(s) 90, 457, 943, 1096, 1121, 1221, 1510
Hpy188III TCNNGA 3 cut(s) 314, 986, 1306
Hpy8I GTNNAC 6 cut(s) 530, 753, 775, 834, 1257, 1328
Hpy99I CGWCG 3 cut(s) 399, 956, 992
HpyAV CCTTC 4 cut(s) 452, 1312, 1336, 1423
HpyCH4III ACNGT 7 cut(s) 137, 160, 190, 721, 1274, 1313, 1415
HpyCH4IV ACGT 2 cut(s) 397, 954
HpyF3I CTNAG 6 cut(s) 102, 215, 631, 679, 747, 1218
HpySE526I ACGT 2 cut(s) 397, 954
Hsp92I GRCGYC 1 cut(s) 397
Hsp92II CATG 9 cut(s) 31, 167, 275, 412, 526, 666, 910, 1160, 1429
HspAI GCGC 4 cut(s) 10, 62, 69, 935
Kpn2I TCCGGA 1 cut(s) 313
Ksp22I TGATCA 1 cut(s) 970
LmnI GCTCC 1 cut(s) 871
Lsp1109I GCAGC 6 cut(s) 109, 302, 1160, 1182, 1472, 1553
LweI GCATC 5 cut(s) 277, 922, 1220, 1484, 1501
MabI ACCWGGT 1 cut(s) 768
MaeI CTAG 2 cut(s) 338, 467
MaeII ACGT 2 cut(s) 397, 954
MaeIII GTNAC 8 cut(s) 154, 167, 190, 206, 665, 1042, 1313, 1415
MboII GAAGA 7 cut(s) 721, 1196, 1351, 1447, 1458, 1494, 1497
MhlI GDGCHC 4 cut(s) 83, 618, 777, 1330
MlyI GAGTC 4 cut(s) 94, 511, 549, 1328
MmeI TCCRAC 1 cut(s) 68
MnlI CCTC 6 cut(s) 70, 371, 415, 756, 1340, 1495
MroI TCCGGA 1 cut(s) 313
MslI CAYNNNNRTG 4 cut(s) 32, 535, 642, 1430
MspA1I CMGCKG 1 cut(s) 478
MspI CCGG 7 cut(s) 246, 257, 314, 425, 690, 1377, 1472
MspR9I CCNGG 6 cut(s) 66, 247, 426, 654, 770, 1207
MvaI CCWGG 4 cut(s) 66, 654, 770, 1207
MvnI CGCG 2 cut(s) 71, 992
NciI CCSGG 2 cut(s) 247, 426
NcoI CCATGG 1 cut(s) 408
NlaIII CATG 9 cut(s) 31, 167, 275, 412, 526, 666, 910, 1160, 1429
NlaIV GGNNCC 4 cut(s) 244, 382, 414, 550
NmuCI GTSAC 4 cut(s) 190, 1042, 1313, 1415
NsbI TGCGCA 1 cut(s) 11
NspI RCATGY 1 cut(s) 1160
PaeR7I CTCGAG 1 cut(s) 82
PciI ACATGT 1 cut(s) 1156
PfeI GAWTC 2 cut(s) 334, 1187
Pfl23II CGTACG 1 cut(s) 132
PfoI TCCNGGA 1 cut(s) 652
PinAI ACCGGT 1 cut(s) 256
PkrI GCNGC 8 cut(s) 115, 124, 292, 926, 1150, 1172, 1462, 1543
PleI GAGTC 4 cut(s) 93, 511, 549, 1328
PpsI GAGTC 4 cut(s) 93, 511, 549, 1328
PpuMI RGGWCCY 1 cut(s) 1063
PscI ACATGT 1 cut(s) 1156
Psp5II RGGWCCY 1 cut(s) 1063
Psp6I CCWGG 4 cut(s) 64, 652, 768, 1205
PspEI GGTNACC 1 cut(s) 167
PspFI CCCAGC 1 cut(s) 416
PspGI CCWGG 4 cut(s) 64, 652, 768, 1205
PspLI CGTACG 1 cut(s) 132
PspN4I GGNNCC 4 cut(s) 244, 382, 414, 550
PspPI GGNCC 4 cut(s) 381, 412, 549, 1063
PspPPI RGGWCCY 1 cut(s) 1063
PspXI VCTCGAGB 1 cut(s) 82
PstI CTGCAG 4 cut(s) 1450, 1462, 1543, 1591
PstNI CAGNNNCTG 1 cut(s) 1547
PvuII CAGCTG 1 cut(s) 478
RsaI GTAC 8 cut(s) 134, 529, 684, 792, 833, 1256, 1381, 1402
RsaNI GTAC 8 cut(s) 133, 528, 683, 791, 832, 1255, 1380, 1401
RseI CAYNNNNRTG 4 cut(s) 32, 535, 642, 1430
SatI GCNGC 8 cut(s) 114, 123, 291, 925, 1149, 1171, 1461, 1542
Sau96I GGNCC 4 cut(s) 381, 412, 549, 1063
ScaI AGTACT 1 cut(s) 684
SchI GAGTC 4 cut(s) 94, 511, 549, 1328
ScrFI CCNGG 6 cut(s) 66, 247, 426, 654, 770, 1207
SduI GDGCHC 4 cut(s) 83, 618, 777, 1330
SexAI ACCWGGT 1 cut(s) 768
SfaNI GCATC 5 cut(s) 277, 922, 1220, 1484, 1501
SfcI CTRYAG 4 cut(s) 1446, 1458, 1539, 1587
Sfr274I CTCGAG 1 cut(s) 82
SgrAI CRCCGGYG 1 cut(s) 256
SinI GGWCC 3 cut(s) 381, 412, 1063
SlaI CTCGAG 1 cut(s) 82
SmiMI CAYNNNNRTG 4 cut(s) 32, 535, 642, 1430
SmlI CTYRAG 2 cut(s) 82, 783
SmoI CTYRAG 2 cut(s) 82, 783
SsiI CCGC 6 cut(s) 71, 113, 542, 869, 924, 992
SspMI CTAG 2 cut(s) 338, 467
StyD4I CCNGG 6 cut(s) 64, 245, 424, 652, 768, 1205
StyI CCWWGG 6 cut(s) 150, 408, 1059, 1174, 1318, 1420
TaaI ACNGT 7 cut(s) 137, 160, 190, 721, 1274, 1313, 1415
TaiI ACGT 2 cut(s) 400, 957
TaqI TCGA 6 cut(s) 83, 600, 951, 1035, 1337, 1487
TatI WGTACW 3 cut(s) 527, 682, 1254
TauI GCSGC 2 cut(s) 116, 927
TfiI GAWTC 2 cut(s) 334, 1187
TscAI CASTG 5 cut(s) 724, 1279, 1335, 1418, 1462
TseFI GTSAC 4 cut(s) 190, 1042, 1313, 1415
TseI GCWGC 6 cut(s) 122, 290, 1148, 1170, 1460, 1541
Tsp45I GTSAC 4 cut(s) 190, 1042, 1313, 1415
TspDTI ATGAA 6 cut(s) 320, 901, 960, 1003, 1113, 1442
TspRI CASTG 5 cut(s) 724, 1279, 1335, 1418, 1462
VneI GTGCAC 2 cut(s) 773, 1326
VpaK11BI GGWCC 3 cut(s) 381, 412, 1063
XapI RAATTY 5 cut(s) 596, 626, 888, 1080, 1223
XceI RCATGY 1 cut(s) 1160
XhoI CTCGAG 1 cut(s) 82
XspI CTAG 2 cut(s) 338, 467
ZraI GACGTC 1 cut(s) 398
ZrmI AGTACT 1 cut(s) 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.