pycom111g01110
ERF Family

Belongs to the AB hydrolase superfamily. Lipase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0000628
Physical Location & Seq
Reverse (-)
701846 .. 704054
2209 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g01110.7

Sequence Viewer

Length: 951 bp
ATGCAGGATGGAGTCACCTGGCTCTTGAATTCTCCGGACAGAAATCTGCCGTTGATTCTGGCGGACGACGGATTTGATGTGTGGATTGCTAACACCAGAGGGACCAGGTTCAGCAGGCGACATACTTCCATGGATCCCCGCGATCCGAAATTTTGGAATTGGTCTTGGGATGAACTGGCGGCTTTTGATCTACCAGCAGTGTTTGATTTCGTGTATGGCAAGGCAGGACAGAAGATCAATTATGTTGGCCATTCCATGGGAACTTTATTTGTATTGGCATCGTTATCAGAAGGGAAACTGGTGGACCAGATGAAATCAGCAGCCTTGCTAAGCCCCATTGCTTATTTGAGCCACATGAACACTGCACTTGGTGTGGCTGCTGCCAAAGCCTTTGTTGGTGAGATCACTACACTTTTCGGACTTGCGGAGTTCAACCCGAAAGGGGACCCAGTGGCTGAATTTCTGAAAGCTCTATGTGCTTATCCAGGAGTTTACTGCTATGACTTACTACAAGCAGTTACTGGCAAAAACTGCTGTCTCAATTCTTCCACAATCGATCTCTTCTTGGAGAATGAGCCTCAGTCAACATCCACCAAGAACATGGTGCACTTGGCTCAAACCGTACGAGATGGGAAGTTGGCAAAATATAACTACGGCAGACCAGACTTAAACTTGATGCATTATGGCGGATTTAACCCTCCAGTTTACAACCTCTCCAACATTCCCCATGACCTTCCTCTGTTCCTCAGCTACGGCGGCCAAGACGCGCTCTCCGATCTTCGCGACGTGGCGCGCTTACTGGATAGTCTAAAACTTCACGATGTGGGGAAGCTCACTGTTCAGTACATAGAGAATTATGCACATGCAGATTTCATCATGGGGTTGAATGCTAAGGACATTGTGTATAATCAAGTGACTGCATTTTTCAAGCAGCAACACCGATTTTACTAA

Protein Analysis

317

Amino Acids

35.31

Weight (kDa)

5.97

Isoelectric Point (pI)

28.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000628)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14180 AT5G14180 AT5G14180 AT5G14180 AT5G14180
fragaria_vesca FvH4_6g41960 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g53200
malus_domestica MD09G1013100.v1.1 MD09G1115500.v1.1 MD09G1115600.v1.1 MD09G1115800.v1.1 MD17G1013800.v1.1 MD17G1107400.v1.1
prunus_persica Prupe.3G208900_v2.0.a1 Prupe.3G208900_v2.0.a1 Prupe.3G304100_v2.0.a1
pyrus_communis pycom09g04000 pycom09g04020 pycom09g04040 pycom111g01090 pycom111g01110 pycom17g01090 pycom17g10010
rosa_chinensis RchiOBHm_Chr2g0157861 RchiOBHm_Chr2g0157881 RchiOBHm_Chr2g0175241 RchiOBHm_Chr2g0175251
rosa_laevigata RLG00000021032 RLG00000021033 RLG00000022314 RLG00000022315
rosa_multiflora Rmu_co8368271.1_g000001 Rmu_co8519319.1_g000001 Rmu_co8519319.1_g000002 Rmu_sc0002441.1_g000007 Rmu_sc0002441.1_g000008 Rmu_sc0010083.1_g000002 Rmu_sc0015795.1_g000001 Rmu_sc0015795.1_g000002
rosa_roxburghii Rroxscaffold_1G00036740 Rroxscaffold_2G00077450 Rroxscaffold_2G00077460 Rroxscaffold_2G00091880 Rroxscaffold_2G00091890 Rroxscaffold_2G00127210
rosa_rugosa Rorug02G0464200 Rorug02G0464200 Rorug02G0582000 Rorug02G0582100 Rorug02G0582100 Rorug02G0582100 Rorug02G0582200 Rorug02G0582200 Rorug02G0582200
rosa_samantha Rh2AG529400 Rh2AG529500 Rh2AG662400 Rh2AG662500 Rh2BG544000 Rh2BG544100 Rh2BG673600 Rh2BG673700 Rh2CG513700 Rh2CG513800 Rh2CG636800 Rh2DG552400
rosa_wichuraiana Rw2G043800 Rw2G043810 Rw2G054330 Rw2G054340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 256
AccII CGCG 4 cut(s) 141, 767, 783, 793
AccIII TCCGGA 1 cut(s) 34
AciI CCGC 6 cut(s) 62, 139, 179, 425, 687, 756
AclWI GGATC 3 cut(s) 128, 137, 141
AcoI YGGCCR 2 cut(s) 247, 757
AcsI RAATTY 3 cut(s) 28, 149, 458
AdeI CACNNNGTG 2 cut(s) 371, 823
AfaI GTAC 2 cut(s) 624, 845
AfiI CCNNNNNNNGG 2 cut(s) 256, 442
AgsI TTSAA 4 cut(s) 28, 433, 886, 928
AjiI CACGTC 1 cut(s) 787
AjnI CCWGG 3 cut(s) 17, 104, 484
AluBI AGCT 3 cut(s) 470, 750, 832
AluI AGCT 3 cut(s) 470, 750, 832
Alw21I GWGCWC 1 cut(s) 609
Alw26I GTCTC 1 cut(s) 542
Alw44I GTGCAC 1 cut(s) 605
AlwI GGATC 3 cut(s) 128, 137, 141
AlwNI CAGNNNCTG 2 cut(s) 455, 521
Aor13HI TCCGGA 1 cut(s) 34
AoxI GGCC 2 cut(s) 247, 757
ApaLI GTGCAC 1 cut(s) 605
ApeKI GCWGC 4 cut(s) 320, 377, 380, 931
ApoI RAATTY 3 cut(s) 28, 149, 458
ArsI GACNNNNNNTTYG 2 cut(s) 56, 88
AspLEI GCGC 3 cut(s) 769, 793, 795
AspS9I GGNCC 3 cut(s) 102, 304, 445
AsuHPI GGTGA 2 cut(s) 7, 410
AvaII GGWCC 3 cut(s) 102, 304, 445
BaeGI GKGCMC 1 cut(s) 609
BalI TGGCCA 1 cut(s) 249
BamHI GGATCC 1 cut(s) 133
Bbv12I GWGCWC 1 cut(s) 609
BbvCI CCTCAGC 1 cut(s) 746
BbvI GCAGC 4 cut(s) 332, 364, 367, 943
BccI CCATC 2 cut(s) 2, 623
BceAI ACGGC 3 cut(s) 34, 670, 769
BciT130I CCWGG 3 cut(s) 19, 106, 486
BcoDI GTCTC 1 cut(s) 542
BisI GCNGC 6 cut(s) 180, 321, 378, 381, 757, 932
BlpI GCTNAGC 1 cut(s) 329
BlsI GCNGC 6 cut(s) 181, 322, 379, 382, 758, 933
Bme1390I CCNGG 3 cut(s) 19, 106, 486
Bme18I GGWCC 3 cut(s) 102, 304, 445
BmgBI CACGTC 1 cut(s) 787
BmgT120I GGNCC 3 cut(s) 102, 304, 445
BmiI GGNNCC 4 cut(s) 103, 135, 446, 447
BmrFI CCNGG 3 cut(s) 19, 106, 486
BmrI ACTGGG 1 cut(s) 443
BmsI GCATC 2 cut(s) 287, 666
BmuI ACTGGG 1 cut(s) 443
BpmI CTGGAG 1 cut(s) 684
Bpu10I CCTNAGC 2 cut(s) 746, 891
Bpu1102I GCTNAGC 1 cut(s) 329
Bsa29I ATCGAT 1 cut(s) 555
BsaJI CCNNGG 2 cut(s) 129, 255
BsaWI WCCGGW 1 cut(s) 34
BsaXI ACNNNNNCTCC 4 cut(s) 698, 728, 755, 785
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 442
Bse1I ACTGG 6 cut(s) 180, 303, 449, 526, 701, 804
Bse3DI GCAATG 1 cut(s) 336
BseAI TCCGGA 1 cut(s) 34
BseBI CCWGG 3 cut(s) 19, 106, 486
BseCI ATCGAT 1 cut(s) 555
BseDI CCNNGG 2 cut(s) 129, 255
BseGI GGATG 3 cut(s) 13, 175, 587
BseLI CCNNNNNNNGG 2 cut(s) 256, 442
BseMI GCAATG 1 cut(s) 336
BseMII CTCAG 2 cut(s) 593, 760
BseNI ACTGG 6 cut(s) 180, 303, 449, 526, 701, 804
BsePI GCGCGC 1 cut(s) 791
BseSI GKGCMC 1 cut(s) 609
BseXI GCAGC 4 cut(s) 332, 364, 367, 943
BsgI GTGCAG 1 cut(s) 348
Bsh1236I CGCG 4 cut(s) 141, 767, 783, 793
BshFI GGCC 2 cut(s) 249, 759
BshVI ATCGAT 1 cut(s) 555
BsiHKAI GWGCWC 1 cut(s) 609
BsiSI CCGG 1 cut(s) 35
BsiWI CGTACG 1 cut(s) 622
BslFI GGGAC 2 cut(s) 115, 458
BslI CCNNNNNNNGG 2 cut(s) 256, 442
BsmAI GTCTC 1 cut(s) 542
BsmFI GGGAC 2 cut(s) 115, 458
BsmI GAATGC 1 cut(s) 892
BsnI GGCC 2 cut(s) 249, 759
Bsp1286I GDGCHC 1 cut(s) 609
Bsp13I TCCGGA 1 cut(s) 34
Bsp143I GATC 7 cut(s) 133, 142, 187, 234, 402, 556, 775
Bsp1720I GCTNAGC 1 cut(s) 329
Bsp19I CCATGG 2 cut(s) 129, 255
Bsp68I TCGCGA 1 cut(s) 783
BspACI CCGC 6 cut(s) 62, 139, 179, 425, 687, 756
BspANI GGCC 2 cut(s) 249, 759
BspCNI CTCAG 2 cut(s) 592, 759
BspDI ATCGAT 1 cut(s) 555
BspEI TCCGGA 1 cut(s) 34
BspFNI CGCG 4 cut(s) 141, 767, 783, 793
BspLI GGNNCC 4 cut(s) 103, 135, 446, 447
BspPI GGATC 3 cut(s) 128, 137, 141
BsrDI GCAATG 1 cut(s) 336
BsrI ACTGG 6 cut(s) 180, 303, 449, 526, 701, 804
BssECI CCNNGG 2 cut(s) 129, 255
BssHII GCGCGC 1 cut(s) 791
BssMI GATC 7 cut(s) 133, 142, 187, 234, 402, 556, 775
BssT1I CCWWGG 2 cut(s) 129, 255
Bst2UI CCWGG 3 cut(s) 19, 106, 486
Bst4CI ACNGT 2 cut(s) 622, 838
Bst6I CTCTTC 1 cut(s) 566
BstAPI GCANNNNNTGC 1 cut(s) 531
BstC8I GCNNGC 2 cut(s) 116, 793
BstDEI CTNAG 4 cut(s) 329, 579, 746, 891
BstDSI CCRYGG 2 cut(s) 129, 255
BstF5I GGATG 3 cut(s) 13, 175, 587
BstFNI CGCG 4 cut(s) 141, 767, 783, 793
BstHHI GCGC 3 cut(s) 769, 793, 795
BstKTI GATC 7 cut(s) 136, 145, 190, 237, 405, 559, 778
BstMAI GTCTC 1 cut(s) 542
BstMBI GATC 7 cut(s) 133, 142, 187, 234, 402, 556, 775
BstMWI GCNNNNNNNGC 4 cut(s) 386, 476, 531, 756
BstNI CCWGG 3 cut(s) 19, 106, 486
BstNSI RCATGY 1 cut(s) 866
BstSCI CCNGG 3 cut(s) 17, 104, 484
BstSLI GKGCMC 1 cut(s) 609
BstUI CGCG 4 cut(s) 141, 767, 783, 793
BstV1I GCAGC 4 cut(s) 332, 364, 367, 943
BstX2I RGATCY 1 cut(s) 133
BstXI CCANNNNNNTGG 1 cut(s) 601
BstYI RGATCY 1 cut(s) 133
Bsu15I ATCGAT 1 cut(s) 555
BsuRI GGCC 2 cut(s) 249, 759
BsuTUI ATCGAT 1 cut(s) 555
BtgI CCRYGG 2 cut(s) 129, 255
BtrI CACGTC 1 cut(s) 787
BtsCI GGATG 3 cut(s) 13, 175, 587
BtsI GCAGTG 2 cut(s) 204, 360
BtsIMutI CAGTG 4 cut(s) 204, 360, 456, 834
BtuMI TCGCGA 1 cut(s) 783
Cac8I GCNNGC 2 cut(s) 116, 793
CaiI CAGNNNCTG 2 cut(s) 455, 521
CfoI GCGC 3 cut(s) 769, 793, 795
Cfr13I GGNCC 3 cut(s) 102, 304, 445
ClaI ATCGAT 1 cut(s) 555
CseI GACGC 1 cut(s) 773
CsiI ACCWGGT 1 cut(s) 104
Csp6I GTAC 2 cut(s) 623, 844
CviAII CATG 7 cut(s) 130, 256, 355, 601, 728, 863, 877
CviQI GTAC 2 cut(s) 623, 844
DdeI CTNAG 4 cut(s) 329, 579, 746, 891
DpnI GATC 7 cut(s) 135, 144, 189, 236, 404, 558, 777
DpnII GATC 7 cut(s) 133, 142, 187, 234, 402, 556, 775
DraIII CACNNNGTG 2 cut(s) 371, 823
EaeI YGGCCR 2 cut(s) 247, 757
Eam1104I CTCTTC 1 cut(s) 566
EarI CTCTTC 1 cut(s) 566
EciI GGCGGA 2 cut(s) 77, 702
Eco130I CCWWGG 2 cut(s) 129, 255
Eco47I GGWCC 3 cut(s) 102, 304, 445
EcoO109I RGGNCCY 1 cut(s) 445
EcoRI GAATTC 1 cut(s) 28
EcoRII CCWGG 3 cut(s) 17, 104, 484
EcoT14I CCWWGG 2 cut(s) 129, 255
EcoT22I ATGCAT 1 cut(s) 681
ErhI CCWWGG 2 cut(s) 129, 255
FaeI CATG 7 cut(s) 133, 259, 358, 604, 731, 866, 880
FaqI GGGAC 2 cut(s) 115, 458
FatI CATG 7 cut(s) 129, 255, 354, 600, 727, 862, 876
FauI CCCGC 1 cut(s) 146
Fnu4HI GCNGC 6 cut(s) 180, 321, 378, 381, 757, 932
FokI GGATG 3 cut(s) 20, 182, 574
Fsp4HI GCNGC 6 cut(s) 180, 321, 378, 381, 757, 932
GlaI GCGC 3 cut(s) 768, 792, 794
GluI GCNGC 6 cut(s) 180, 321, 378, 381, 757, 932
GsuI CTGGAG 1 cut(s) 684
HaeIII GGCC 2 cut(s) 249, 759
HapII CCGG 1 cut(s) 35
HgaI GACGC 1 cut(s) 773
HhaI GCGC 3 cut(s) 769, 793, 795
Hin1II CATG 7 cut(s) 133, 259, 358, 604, 731, 866, 880
Hin6I GCGC 3 cut(s) 767, 791, 793
HinP1I GCGC 3 cut(s) 767, 791, 793
HincII GTYRAC 1 cut(s) 585
HindII GTYRAC 1 cut(s) 585
HinfI GANTC 2 cut(s) 12, 55
HpaII CCGG 1 cut(s) 35
HphI GGTGA 2 cut(s) 7, 410
Hpy166II GTNNAC 5 cut(s) 304, 493, 585, 607, 706
Hpy188I TCNGA 5 cut(s) 147, 289, 419, 465, 775
Hpy188III TCNNGA 4 cut(s) 25, 35, 782, 818
Hpy8I GTNNAC 5 cut(s) 304, 493, 585, 607, 706
Hpy99I CGWCG 2 cut(s) 71, 788
HpyAV CCTTC 2 cut(s) 284, 743
HpyCH4III ACNGT 2 cut(s) 622, 838
HpyCH4IV ACGT 1 cut(s) 786
HpyCH4V TGCA 7 cut(s) 4, 365, 607, 679, 860, 866, 920
HpyF10VI GCNNNNNNNGC 4 cut(s) 386, 476, 531, 756
HpyF3I CTNAG 4 cut(s) 329, 579, 746, 891
HpySE526I ACGT 1 cut(s) 786
Hsp92II CATG 7 cut(s) 133, 259, 358, 604, 731, 866, 880
HspAI GCGC 3 cut(s) 767, 791, 793
KflI GGGWCCC 1 cut(s) 445
Kpn2I TCCGGA 1 cut(s) 34
Kzo9I GATC 7 cut(s) 133, 142, 187, 234, 402, 556, 775
Lsp1109I GCAGC 4 cut(s) 332, 364, 367, 943
LweI GCATC 2 cut(s) 287, 666
MabI ACCWGGT 1 cut(s) 104
MaeII ACGT 1 cut(s) 786
MaeIII GTNAC 3 cut(s) 13, 517, 913
MalI GATC 7 cut(s) 135, 144, 189, 236, 404, 558, 777
MboI GATC 7 cut(s) 133, 142, 187, 234, 402, 556, 775
MboII GAAGA 4 cut(s) 244, 537, 553, 770
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 1 cut(s) 609
MlsI TGGCCA 1 cut(s) 249
MluCI AATT 7 cut(s) 28, 149, 157, 238, 458, 541, 853
MluNI TGGCCA 1 cut(s) 249
MlyI GAGTC 1 cut(s) 21
MmeI TCCRAC 1 cut(s) 741
MnlI CCTC 6 cut(s) 92, 588, 708, 722, 747, 755
Mox20I TGGCCA 1 cut(s) 249
Mph1103I ATGCAT 1 cut(s) 681
MroI TCCGGA 1 cut(s) 34
MscI TGGCCA 1 cut(s) 249
MseI TTAA 2 cut(s) 668, 693
Msp20I TGGCCA 1 cut(s) 249
MspI CCGG 1 cut(s) 35
MspR9I CCNGG 3 cut(s) 19, 106, 486
Mva1269I GAATGC 1 cut(s) 892
MvaI CCWGG 3 cut(s) 19, 106, 486
MvnI CGCG 4 cut(s) 141, 767, 783, 793
MwoI GCNNNNNNNGC 4 cut(s) 386, 476, 531, 756
NcoI CCATGG 2 cut(s) 129, 255
NdeII GATC 7 cut(s) 133, 142, 187, 234, 402, 556, 775
NlaIII CATG 7 cut(s) 133, 259, 358, 604, 731, 866, 880
NlaIV GGNNCC 4 cut(s) 103, 135, 446, 447
NmuCI GTSAC 2 cut(s) 13, 913
NruI TCGCGA 1 cut(s) 783
NsiI ATGCAT 1 cut(s) 681
NspI RCATGY 1 cut(s) 866
PauI GCGCGC 1 cut(s) 791
PcsI WCGNNNNNNNCGW 1 cut(s) 771
PctI GAATGC 1 cut(s) 892
PfeI GAWTC 1 cut(s) 55
Pfl23II CGTACG 1 cut(s) 622
PflMI CCANNNNNTGG 1 cut(s) 256
PfoI TCCNGGA 1 cut(s) 484
PkrI GCNGC 6 cut(s) 181, 322, 379, 382, 758, 933
PleI GAGTC 1 cut(s) 20
PpsI GAGTC 1 cut(s) 20
PpuMI RGGWCCY 1 cut(s) 445
Psp5II RGGWCCY 1 cut(s) 445
Psp6I CCWGG 3 cut(s) 17, 104, 484
PspGI CCWGG 3 cut(s) 17, 104, 484
PspLI CGTACG 1 cut(s) 622
PspN4I GGNNCC 4 cut(s) 103, 135, 446, 447
PspPI GGNCC 3 cut(s) 102, 304, 445
PspPPI RGGWCCY 1 cut(s) 445
PstNI CAGNNNCTG 2 cut(s) 455, 521
PsuI RGATCY 1 cut(s) 133
PteI GCGCGC 1 cut(s) 791
RruI TCGCGA 1 cut(s) 783
RsaI GTAC 2 cut(s) 624, 845
RsaNI GTAC 2 cut(s) 623, 844
SaqAI TTAA 2 cut(s) 668, 693
SatI GCNGC 6 cut(s) 180, 321, 378, 381, 757, 932
Sau3AI GATC 7 cut(s) 133, 142, 187, 234, 402, 556, 775
Sau96I GGNCC 3 cut(s) 102, 304, 445
SchI GAGTC 1 cut(s) 21
ScrFI CCNGG 3 cut(s) 19, 106, 486
SduI GDGCHC 1 cut(s) 609
SetI ASST 8 cut(s) 20, 110, 472, 714, 735, 752, 789, 834
SexAI ACCWGGT 1 cut(s) 104
SfaNI GCATC 2 cut(s) 287, 666
SinI GGWCC 3 cut(s) 102, 304, 445
Sse9I AATT 7 cut(s) 28, 149, 157, 238, 458, 541, 853
SsiI CCGC 6 cut(s) 62, 139, 179, 425, 687, 756
StyD4I CCNGG 3 cut(s) 17, 104, 484
StyI CCWWGG 2 cut(s) 129, 255
TaaI ACNGT 2 cut(s) 622, 838
TaiI ACGT 1 cut(s) 789
TaqI TCGA 1 cut(s) 555
TasI AATT 7 cut(s) 28, 149, 157, 238, 458, 541, 853
TatI WGTACW 1 cut(s) 843
TauI GCSGC 2 cut(s) 182, 759
TfiI GAWTC 1 cut(s) 55
Tru1I TTAA 2 cut(s) 668, 693
Tru9I TTAA 2 cut(s) 668, 693
TscAI CASTG 4 cut(s) 204, 367, 456, 841
TseFI GTSAC 2 cut(s) 13, 913
TseI GCWGC 4 cut(s) 320, 377, 380, 931
Tsp45I GTSAC 2 cut(s) 13, 913
TspDTI ATGAA 4 cut(s) 186, 326, 371, 862
TspGWI ACGGA 1 cut(s) 84
TspRI CASTG 4 cut(s) 204, 367, 456, 841
Van91I CCANNNNNTGG 1 cut(s) 256
VneI GTGCAC 1 cut(s) 605
VpaK11BI GGWCC 3 cut(s) 102, 304, 445
XapI RAATTY 3 cut(s) 28, 149, 458
XceI RCATGY 1 cut(s) 866
XcmI CCANNNNNNNNNTGG 1 cut(s) 598
Zsp2I ATGCAT 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.