pycom111g01090
ERF Family

Belongs to the AB hydrolase superfamily. Lipase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0000628
Physical Location & Seq
Reverse (-)
691795 .. 692991
1197 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g01090.4

Sequence Viewer

Length: 633 bp
ATGAAATCAGCAGCCTTGCTAAGCCCCATTGCTTATTTGAGCCACATGAACACTGCACTTGGTGTGGCTGCTGCCAAAGCCTTTGTTGGTGAGATCACTACGCTTTTCGGACTTGCGGAGTTCAACCTGAAAGGGGACCCAGCGGCTCAATTTCTGAAAGCTCTATGCGCTTATCCATGGGTTTACTGCTATGACTTACTACAAGGACTTGCTGGCAAAAACTGCTGTCTCAATTATTCCACCATCGATCTCTTCTTGGAGAATGAGCCTCAGTCAACATCCACCAAGAACATGGTGCACTTGGCTCAAACTGTACGAGATGGGAAGTTGGCAAAATATAACTACGGCAGACCAGACTTAAACTTGATGCATTATGGCGGATTTAACCCTCCAGTTTACAATCTCTCCAACATTCCCCATGACCTTCCTCTGTTCCTCAGCTACGGCGGCCAAGACTCGCTCTCCGATCTTCGTGACGTGGCGCGCTTACTGGATAGTCTAAAACTTCACGATGTGGGGAAGCTCACTGTTCAGTACATAGAGAATTATGCACATGCAGATTTCATCATGGGGTTGAATGCTAAGGACATTGTGTATAGTCAAGTGACTGCATTTTTCAAGCAGCAACACTGA

Protein Analysis

211

Amino Acids

23.28

Weight (kDa)

6.36

Isoelectric Point (pI)

30.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000628)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14180 AT5G14180 AT5G14180 AT5G14180 AT5G14180
fragaria_vesca FvH4_6g41960 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g53200
malus_domestica MD09G1013100.v1.1 MD09G1115500.v1.1 MD09G1115600.v1.1 MD09G1115800.v1.1 MD17G1013800.v1.1 MD17G1107400.v1.1
prunus_persica Prupe.3G208900_v2.0.a1 Prupe.3G208900_v2.0.a1 Prupe.3G304100_v2.0.a1
pyrus_communis pycom09g04000 pycom09g04020 pycom09g04040 pycom111g01090 pycom111g01110 pycom17g01090 pycom17g10010
rosa_chinensis RchiOBHm_Chr2g0157861 RchiOBHm_Chr2g0157881 RchiOBHm_Chr2g0175241 RchiOBHm_Chr2g0175251
rosa_laevigata RLG00000021032 RLG00000021033 RLG00000022314 RLG00000022315
rosa_multiflora Rmu_co8368271.1_g000001 Rmu_co8519319.1_g000001 Rmu_co8519319.1_g000002 Rmu_sc0002441.1_g000007 Rmu_sc0002441.1_g000008 Rmu_sc0010083.1_g000002 Rmu_sc0015795.1_g000001 Rmu_sc0015795.1_g000002
rosa_roxburghii Rroxscaffold_1G00036740 Rroxscaffold_2G00077450 Rroxscaffold_2G00077460 Rroxscaffold_2G00091880 Rroxscaffold_2G00091890 Rroxscaffold_2G00127210
rosa_rugosa Rorug02G0464200 Rorug02G0464200 Rorug02G0582000 Rorug02G0582100 Rorug02G0582100 Rorug02G0582100 Rorug02G0582200 Rorug02G0582200 Rorug02G0582200
rosa_samantha Rh2AG529400 Rh2AG529500 Rh2AG662400 Rh2AG662500 Rh2BG544000 Rh2BG544100 Rh2BG673600 Rh2BG673700 Rh2CG513700 Rh2CG513800 Rh2CG636800 Rh2DG552400
rosa_wichuraiana Rw2G043800 Rw2G043810 Rw2G054330 Rw2G054340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 484
AciI CCGC 4 cut(s) 116, 143, 378, 447
AcoI YGGCCR 1 cut(s) 448
AdeI CACNNNGTG 2 cut(s) 62, 514
AfaI GTAC 2 cut(s) 315, 536
AfiI CCNNNNNNNGG 1 cut(s) 133
AgsI TTSAA 3 cut(s) 124, 577, 619
AjiI CACGTC 1 cut(s) 478
AluBI AGCT 3 cut(s) 161, 441, 523
AluI AGCT 3 cut(s) 161, 441, 523
Alw21I GWGCWC 1 cut(s) 300
Alw26I GTCTC 1 cut(s) 233
Alw44I GTGCAC 1 cut(s) 296
AoxI GGCC 1 cut(s) 448
ApaLI GTGCAC 1 cut(s) 296
ApeKI GCWGC 4 cut(s) 11, 68, 71, 622
AspLEI GCGC 3 cut(s) 170, 484, 486
AspS9I GGNCC 1 cut(s) 136
AsuHPI GGTGA 1 cut(s) 101
AvaII GGWCC 1 cut(s) 136
BaeGI GKGCMC 1 cut(s) 300
Bbv12I GWGCWC 1 cut(s) 300
BbvCI CCTCAGC 1 cut(s) 437
BbvI GCAGC 3 cut(s) 23, 55, 58
BccI CCATC 2 cut(s) 251, 314
BceAI ACGGC 2 cut(s) 361, 460
BcoDI GTCTC 1 cut(s) 233
BisI GCNGC 6 cut(s) 12, 69, 72, 144, 448, 623
BlpI GCTNAGC 1 cut(s) 20
BlsI GCNGC 6 cut(s) 13, 70, 73, 145, 449, 624
Bme18I GGWCC 1 cut(s) 136
BmgBI CACGTC 1 cut(s) 478
BmgT120I GGNCC 1 cut(s) 136
BmiI GGNNCC 2 cut(s) 137, 138
BmsI GCATC 1 cut(s) 357
BpmI CTGGAG 1 cut(s) 375
Bpu10I CCTNAGC 2 cut(s) 437, 582
Bpu1102I GCTNAGC 1 cut(s) 20
Bsa29I ATCGAT 1 cut(s) 246
BsaJI CCNNGG 1 cut(s) 176
BsaXI ACNNNNNCTCC 4 cut(s) 389, 419, 446, 476
Bsc4I CCNNNNNNNGG 1 cut(s) 133
Bse1I ACTGG 2 cut(s) 392, 495
Bse3DI GCAATG 1 cut(s) 27
BseCI ATCGAT 1 cut(s) 246
BseDI CCNNGG 1 cut(s) 176
BseGI GGATG 1 cut(s) 278
BseLI CCNNNNNNNGG 1 cut(s) 133
BseMI GCAATG 1 cut(s) 27
BseMII CTCAG 2 cut(s) 284, 451
BseNI ACTGG 2 cut(s) 392, 495
BsePI GCGCGC 1 cut(s) 482
BseSI GKGCMC 1 cut(s) 300
BseXI GCAGC 3 cut(s) 23, 55, 58
BseYI CCCAGC 1 cut(s) 139
BsgI GTGCAG 1 cut(s) 39
Bsh1236I CGCG 1 cut(s) 484
BshFI GGCC 1 cut(s) 450
BshVI ATCGAT 1 cut(s) 246
BsiHKAI GWGCWC 1 cut(s) 300
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 1 cut(s) 133
BsmAI GTCTC 1 cut(s) 233
BsmFI GGGAC 1 cut(s) 149
BsmI GAATGC 1 cut(s) 583
BsnI GGCC 1 cut(s) 450
Bsp1286I GDGCHC 1 cut(s) 300
Bsp143I GATC 3 cut(s) 93, 247, 466
Bsp1720I GCTNAGC 1 cut(s) 20
Bsp19I CCATGG 1 cut(s) 176
BspACI CCGC 4 cut(s) 116, 143, 378, 447
BspANI GGCC 1 cut(s) 450
BspCNI CTCAG 2 cut(s) 283, 450
BspDI ATCGAT 1 cut(s) 246
BspFNI CGCG 1 cut(s) 484
BspLI GGNNCC 2 cut(s) 137, 138
BsrDI GCAATG 1 cut(s) 27
BsrI ACTGG 2 cut(s) 392, 495
BssECI CCNNGG 1 cut(s) 176
BssHII GCGCGC 1 cut(s) 482
BssMI GATC 3 cut(s) 93, 247, 466
BssT1I CCWWGG 1 cut(s) 176
Bst4CI ACNGT 2 cut(s) 313, 529
Bst6I CTCTTC 1 cut(s) 257
BstAPI GCANNNNNTGC 1 cut(s) 222
BstC8I GCNNGC 2 cut(s) 214, 484
BstDEI CTNAG 4 cut(s) 20, 270, 437, 582
BstDSI CCRYGG 1 cut(s) 176
BstF5I GGATG 1 cut(s) 278
BstFNI CGCG 1 cut(s) 484
BstHHI GCGC 3 cut(s) 170, 484, 486
BstKTI GATC 3 cut(s) 96, 250, 469
BstMAI GTCTC 1 cut(s) 233
BstMBI GATC 3 cut(s) 93, 247, 466
BstMWI GCNNNNNNNGC 4 cut(s) 77, 167, 222, 447
BstNSI RCATGY 1 cut(s) 557
BstSLI GKGCMC 1 cut(s) 300
BstUI CGCG 1 cut(s) 484
BstV1I GCAGC 3 cut(s) 23, 55, 58
BstXI CCANNNNNNTGG 1 cut(s) 292
Bsu15I ATCGAT 1 cut(s) 246
BsuRI GGCC 1 cut(s) 450
BsuTUI ATCGAT 1 cut(s) 246
BtgI CCRYGG 1 cut(s) 176
BtrI CACGTC 1 cut(s) 478
BtsCI GGATG 1 cut(s) 278
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 3 cut(s) 51, 525, 628
Cac8I GCNNGC 2 cut(s) 214, 484
CfoI GCGC 3 cut(s) 170, 484, 486
Cfr13I GGNCC 1 cut(s) 136
ClaI ATCGAT 1 cut(s) 246
Csp6I GTAC 2 cut(s) 314, 535
CviAII CATG 6 cut(s) 46, 177, 292, 419, 554, 568
CviQI GTAC 2 cut(s) 314, 535
DdeI CTNAG 4 cut(s) 20, 270, 437, 582
DpnI GATC 3 cut(s) 95, 249, 468
DpnII GATC 3 cut(s) 93, 247, 466
DraIII CACNNNGTG 2 cut(s) 62, 514
EaeI YGGCCR 1 cut(s) 448
Eam1104I CTCTTC 1 cut(s) 257
EarI CTCTTC 1 cut(s) 257
EciI GGCGGA 1 cut(s) 393
Eco130I CCWWGG 1 cut(s) 176
Eco47I GGWCC 1 cut(s) 136
EcoO109I RGGNCCY 1 cut(s) 136
EcoT14I CCWWGG 1 cut(s) 176
EcoT22I ATGCAT 1 cut(s) 372
ErhI CCWWGG 1 cut(s) 176
FaeI CATG 6 cut(s) 49, 180, 295, 422, 557, 571
FaqI GGGAC 1 cut(s) 149
FatI CATG 6 cut(s) 45, 176, 291, 418, 553, 567
Fnu4HI GCNGC 6 cut(s) 12, 69, 72, 144, 448, 623
FokI GGATG 1 cut(s) 265
Fsp4HI GCNGC 6 cut(s) 12, 69, 72, 144, 448, 623
GlaI GCGC 3 cut(s) 169, 483, 485
GluI GCNGC 6 cut(s) 12, 69, 72, 144, 448, 623
GsaI CCCAGC 1 cut(s) 143
GsuI CTGGAG 1 cut(s) 375
HaeIII GGCC 1 cut(s) 450
HhaI GCGC 3 cut(s) 170, 484, 486
Hin1II CATG 6 cut(s) 49, 180, 295, 422, 557, 571
Hin6I GCGC 3 cut(s) 168, 482, 484
HinP1I GCGC 3 cut(s) 168, 482, 484
HincII GTYRAC 1 cut(s) 276
HindII GTYRAC 1 cut(s) 276
HinfI GANTC 1 cut(s) 455
HphI GGTGA 1 cut(s) 101
Hpy166II GTNNAC 4 cut(s) 184, 276, 298, 397
Hpy188I TCNGA 3 cut(s) 110, 156, 466
Hpy188III TCNNGA 2 cut(s) 473, 509
Hpy8I GTNNAC 4 cut(s) 184, 276, 298, 397
HpyAV CCTTC 1 cut(s) 434
HpyCH4III ACNGT 2 cut(s) 313, 529
HpyCH4IV ACGT 1 cut(s) 477
HpyCH4V TGCA 6 cut(s) 56, 298, 370, 551, 557, 611
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 167, 222, 447
HpyF3I CTNAG 4 cut(s) 20, 270, 437, 582
HpySE526I ACGT 1 cut(s) 477
Hsp92II CATG 6 cut(s) 49, 180, 295, 422, 557, 571
HspAI GCGC 3 cut(s) 168, 482, 484
KflI GGGWCCC 1 cut(s) 136
Kzo9I GATC 3 cut(s) 93, 247, 466
LpnPI CCDG 6 cut(s) 140, 153, 198, 366, 405, 476
Lsp1109I GCAGC 3 cut(s) 23, 55, 58
LweI GCATC 1 cut(s) 357
MaeII ACGT 1 cut(s) 477
MaeIII GTNAC 2 cut(s) 473, 604
MalI GATC 3 cut(s) 95, 249, 468
MboI GATC 3 cut(s) 93, 247, 466
MboII GAAGA 2 cut(s) 244, 461
MhlI GDGCHC 1 cut(s) 300
MluCI AATT 3 cut(s) 149, 232, 544
MlyI GAGTC 1 cut(s) 449
MmeI TCCRAC 1 cut(s) 432
MnlI CCTC 4 cut(s) 279, 399, 438, 446
Mph1103I ATGCAT 1 cut(s) 372
MseI TTAA 2 cut(s) 359, 384
MspA1I CMGCKG 1 cut(s) 143
Mva1269I GAATGC 1 cut(s) 583
MvnI CGCG 1 cut(s) 484
MwoI GCNNNNNNNGC 4 cut(s) 77, 167, 222, 447
NcoI CCATGG 1 cut(s) 176
NdeII GATC 3 cut(s) 93, 247, 466
NlaIII CATG 6 cut(s) 49, 180, 295, 422, 557, 571
NlaIV GGNNCC 2 cut(s) 137, 138
NmuCI GTSAC 2 cut(s) 473, 604
NsiI ATGCAT 1 cut(s) 372
NspI RCATGY 1 cut(s) 557
PauI GCGCGC 1 cut(s) 482
PctI GAATGC 1 cut(s) 583
PkrI GCNGC 6 cut(s) 13, 70, 73, 145, 449, 624
PleI GAGTC 1 cut(s) 449
PpsI GAGTC 1 cut(s) 449
PpuMI RGGWCCY 1 cut(s) 136
Psp5II RGGWCCY 1 cut(s) 136
PspFI CCCAGC 1 cut(s) 139
PspN4I GGNNCC 2 cut(s) 137, 138
PspPI GGNCC 1 cut(s) 136
PspPPI RGGWCCY 1 cut(s) 136
PteI GCGCGC 1 cut(s) 482
RsaI GTAC 2 cut(s) 315, 536
RsaNI GTAC 2 cut(s) 314, 535
SaqAI TTAA 2 cut(s) 359, 384
SatI GCNGC 6 cut(s) 12, 69, 72, 144, 448, 623
Sau3AI GATC 3 cut(s) 93, 247, 466
Sau96I GGNCC 1 cut(s) 136
SchI GAGTC 1 cut(s) 449
SduI GDGCHC 1 cut(s) 300
SetI ASST 6 cut(s) 129, 163, 426, 443, 480, 525
SfaNI GCATC 1 cut(s) 357
SinI GGWCC 1 cut(s) 136
Sse9I AATT 3 cut(s) 149, 232, 544
SsiI CCGC 4 cut(s) 116, 143, 378, 447
StyI CCWWGG 1 cut(s) 176
TaaI ACNGT 2 cut(s) 313, 529
TaiI ACGT 1 cut(s) 480
TaqI TCGA 1 cut(s) 246
TasI AATT 3 cut(s) 149, 232, 544
TatI WGTACW 1 cut(s) 534
TauI GCSGC 2 cut(s) 146, 450
Tru1I TTAA 2 cut(s) 359, 384
Tru9I TTAA 2 cut(s) 359, 384
TscAI CASTG 2 cut(s) 58, 532
TseFI GTSAC 2 cut(s) 473, 604
TseI GCWGC 4 cut(s) 11, 68, 71, 622
Tsp45I GTSAC 2 cut(s) 473, 604
TspDTI ATGAA 3 cut(s) 17, 62, 553
TspRI CASTG 2 cut(s) 58, 532
VneI GTGCAC 1 cut(s) 296
VpaK11BI GGWCC 1 cut(s) 136
XceI RCATGY 1 cut(s) 557
XcmI CCANNNNNNNNNTGG 1 cut(s) 289
Zsp2I ATGCAT 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.