Rroxscaffold_1G00036740
ERF Family

Belongs to the AB hydrolase superfamily. Lipase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000628
Physical Location & Seq
Forward (+)
53668213 .. 53670914
2702 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00036740.1

Sequence Viewer

Length: 1158 bp
ATGGCCTTAGCTTTCTCTGCCTGCATTGGCGTTGTTCTATTCTGGGGATTGGCAGTTGGTACTAGAACAAAGCTATTCTCTTTCGAAGACAGCGTGGTTTCGGCAGTGGCTCCATCTGGTAGTGATGGTATCTGTGCATCAATGGTGCTATCACAAGGCTACCCTTGTGAAGAACATACAGTAACAACACAAGATGGCTACATACTCAGTATGCAGAGAATTCCAGCTGGTAAGTCCGGGGAGACCTCTGGGAACAAGGTACCGGTTCTTCTACAACATGGACTCCTCATGGATGGGATAACATGGCTGCTACTACCTCCAGACGAATCTTTGGCATTCATCTTGGCAGATAATGGGTATGAGGTGTGGATTGCCAATACCCGTGGAACCAAATATAGCCTTGGACATACATCACTCACCCCCGATGATCCGGCTTATTGGGAGTGGTCATGGGATGAATTGGTCGCTTATGACCTCCCAGCCGCATACCAGTATGTGCATGATCAAGCAGGACAGAAGCTTCACTATGTTGGACATTCACTGGGAACTTTGATTGCTCTTGGTGCTTTTTCAGAAGACCAGCCAGTTAACATGTTGAGATCAGCTGTCTTACTTAGCCCAATTGCTCATGTTGGTCAGATAACCTCACCCCTTGCAAAAACTGCTGCTGAAAACTTCATTGCTGAGGAAATCTGCTCCAAACCAGGCGCTGACTGCACAAACTTGATGACCTCTTTCACAGGCCAGAATTGCTGCTTAAATTCATCCATTACCGACATTTTTCTAGACCATGAGCCTCAGGCAACTTCAATCAAAAACATGGTCCACCTCGCTCAGATGATAAGAGATGGAAAAGTTGCGATGTTCGACTACAATGACAAGGAGGAGAACCAACAACACTACGGTCAGGACACCCCTCCAGAGTACAAAATGGCGAGCATACCGCATGACATTCCTCTCTTCCTCGGTTATGGAGGGGCAGATGCGCTTTCAGATGTCCAAGATGTGAAGCTATTGCTCAACAGCCTCAAAGATCATGATGGAGATAAGCTTGTGATTCAGTACATAGATGATTATGCTCATGCAGATTTTGTCATGGGTGAAAATGCTAAACAAGATGTGTATGATCCTCTCATGGCTTTCTTCAAACTTCAGTGA

Protein Analysis

385

Amino Acids

42.13

Weight (kDa)

4.58

Isoelectric Point (pI)

28.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydro_lipase PF04083 47 - 104 1.1e-21 Partial alpha/beta-hydrolase lipase region
Abhydrolase_1 PF00561 88 - 209 5e-09 alpha/beta hydrolase fold
Hydrolase_4 PF12146 111 - 223 6.7e-06 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000628)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14180 AT5G14180 AT5G14180 AT5G14180 AT5G14180
fragaria_vesca FvH4_6g41960 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g53200
malus_domestica MD09G1013100.v1.1 MD09G1115500.v1.1 MD09G1115600.v1.1 MD09G1115800.v1.1 MD17G1013800.v1.1 MD17G1107400.v1.1
prunus_persica Prupe.3G208900_v2.0.a1 Prupe.3G208900_v2.0.a1 Prupe.3G304100_v2.0.a1
pyrus_communis pycom09g04000 pycom09g04020 pycom09g04040 pycom111g01090 pycom111g01110 pycom17g01090 pycom17g10010
rosa_chinensis RchiOBHm_Chr2g0157861 RchiOBHm_Chr2g0157881 RchiOBHm_Chr2g0175241 RchiOBHm_Chr2g0175251
rosa_laevigata RLG00000021032 RLG00000021033 RLG00000022314 RLG00000022315
rosa_multiflora Rmu_co8368271.1_g000001 Rmu_co8519319.1_g000001 Rmu_co8519319.1_g000002 Rmu_sc0002441.1_g000007 Rmu_sc0002441.1_g000008 Rmu_sc0010083.1_g000002 Rmu_sc0015795.1_g000001 Rmu_sc0015795.1_g000002
rosa_roxburghii Rroxscaffold_1G00036740 Rroxscaffold_2G00077450 Rroxscaffold_2G00077460 Rroxscaffold_2G00091880 Rroxscaffold_2G00091890 Rroxscaffold_2G00127210
rosa_rugosa Rorug02G0464200 Rorug02G0464200 Rorug02G0582000 Rorug02G0582100 Rorug02G0582100 Rorug02G0582100 Rorug02G0582200 Rorug02G0582200 Rorug02G0582200
rosa_samantha Rh2AG529400 Rh2AG529500 Rh2AG662400 Rh2AG662500 Rh2BG544000 Rh2BG544100 Rh2BG673600 Rh2BG673700 Rh2CG513700 Rh2CG513800 Rh2CG636800 Rh2DG552400
rosa_wichuraiana Rw2G043800 Rw2G043810 Rw2G054330 Rw2G054340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 259
AccB1I GGYRCC 1 cut(s) 259
AciI CCGC 2 cut(s) 483, 944
AclWI GGATC 2 cut(s) 422, 1121
AcsI RAATTY 2 cut(s) 219, 760
AcuI CTGAAG 1 cut(s) 1136
AfaI GTAC 4 cut(s) 61, 261, 926, 1064
AflIII ACRYGT 1 cut(s) 591
AgeI ACCGGT 1 cut(s) 262
AgsI TTSAA 2 cut(s) 810, 1147
AjnI CCWGG 1 cut(s) 703
AluBI AGCT 7 cut(s) 11, 73, 227, 520, 605, 1012, 1051
AluI AGCT 7 cut(s) 11, 73, 227, 520, 605, 1012, 1051
Alw26I GTCTC 1 cut(s) 236
AlwI GGATC 2 cut(s) 422, 1121
AlwNI CAGNNNCTG 1 cut(s) 710
AoxI GGCC 2 cut(s) 3, 742
ApeKI GCWGC 3 cut(s) 307, 665, 753
ApoI RAATTY 2 cut(s) 219, 760
ArsI GACNNNNNNTTYG 2 cut(s) 807, 839
AsiGI ACCGGT 1 cut(s) 262
Asp718I GGTACC 1 cut(s) 259
AspLEI GCGC 2 cut(s) 710, 988
AspS9I GGNCC 1 cut(s) 823
AsuC2I CCSGG 1 cut(s) 238
AsuHPI GGTGA 3 cut(s) 409, 639, 1112
AsuII TTCGAA 1 cut(s) 84
AvaII GGWCC 1 cut(s) 823
AxyI CCTNAGG 1 cut(s) 798
BanI GGYRCC 1 cut(s) 259
BarI GAAGNNNNNNTAC 2 cut(s) 252, 284
BbsI GAAGAC 2 cut(s) 93, 582
BbvCI CCTCAGC 1 cut(s) 684
BbvI GCAGC 3 cut(s) 294, 652, 740
BccI CCATC 6 cut(s) 119, 121, 188, 287, 842, 1034
BciT130I CCWGG 1 cut(s) 705
BclI TGATCA 1 cut(s) 502
BcnI CCSGG 1 cut(s) 238
BcoDI GTCTC 1 cut(s) 236
BfaI CTAG 2 cut(s) 63, 785
BfoI RGCGCY 1 cut(s) 711
BisI GCNGC 4 cut(s) 308, 483, 666, 754
BlsI GCNGC 4 cut(s) 309, 484, 667, 755
Bme1390I CCNGG 2 cut(s) 238, 705
Bme18I GGWCC 1 cut(s) 823
BmgT120I GGNCC 1 cut(s) 823
BmiI GGNNCC 3 cut(s) 111, 261, 388
BmrFI CCNGG 2 cut(s) 238, 705
BmrI ACTGGG 1 cut(s) 551
BmsI GCATC 2 cut(s) 146, 973
BmuI ACTGGG 1 cut(s) 551
BpiI GAAGAC 2 cut(s) 93, 582
BpmI CTGGAG 2 cut(s) 303, 903
Bpu10I CCTNAGC 2 cut(s) 7, 684
Bpu14I TTCGAA 1 cut(s) 84
BpuMI CCSGG 1 cut(s) 238
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 4 cut(s) 237, 382, 400, 964
BsaWI WCCGGW 1 cut(s) 262
BsaXI ACNNNNNCTCC 2 cut(s) 267, 297
Bse118I RCCGGY 1 cut(s) 262
Bse1I ACTGG 3 cut(s) 490, 546, 584
Bse21I CCTNAGG 1 cut(s) 798
Bse3DI GCAATG 1 cut(s) 678
BseBI CCWGG 1 cut(s) 705
BseDI CCNNGG 4 cut(s) 237, 382, 400, 964
BseGI GGATG 3 cut(s) 298, 460, 764
BseMI GCAATG 1 cut(s) 678
BseMII CTCAG 4 cut(s) 220, 675, 812, 848
BseNI ACTGG 3 cut(s) 490, 546, 584
BseRI GAGGAG 2 cut(s) 275, 899
BseXI GCAGC 3 cut(s) 294, 652, 740
BseYI CCCAGC 1 cut(s) 478
BsgI GTGCAG 1 cut(s) 700
BshFI GGCC 2 cut(s) 5, 744
BshNI GGYRCC 1 cut(s) 259
BshTI ACCGGT 1 cut(s) 262
BsiSI CCGG 3 cut(s) 237, 263, 431
BsmAI GTCTC 1 cut(s) 236
BsmI GAATGC 1 cut(s) 335
BsnI GGCC 2 cut(s) 5, 744
Bso31I GGTCTC 1 cut(s) 236
Bsp119I TTCGAA 1 cut(s) 84
Bsp143I GATC 5 cut(s) 427, 502, 599, 1033, 1126
BspACI CCGC 2 cut(s) 483, 944
BspANI GGCC 2 cut(s) 5, 744
BspCNI CTCAG 4 cut(s) 219, 676, 811, 847
BspHI TCATGA 1 cut(s) 1036
BspLI GGNNCC 3 cut(s) 111, 261, 388
BspPI GGATC 2 cut(s) 422, 1121
BspT104I TTCGAA 1 cut(s) 84
BspT107I GGYRCC 1 cut(s) 259
BspTNI GGTCTC 1 cut(s) 236
BsrDI GCAATG 1 cut(s) 678
BsrFI RCCGGY 1 cut(s) 262
BsrI ACTGG 3 cut(s) 490, 546, 584
BssAI RCCGGY 1 cut(s) 262
BssECI CCNNGG 4 cut(s) 237, 382, 400, 964
BssMI GATC 5 cut(s) 427, 502, 599, 1033, 1126
BssT1I CCWWGG 1 cut(s) 400
Bst2UI CCWGG 1 cut(s) 705
Bst4CI ACNGT 2 cut(s) 181, 905
Bst6I CTCTTC 1 cut(s) 965
BstAPI GCANNNNNTGC 1 cut(s) 662
BstBI TTCGAA 1 cut(s) 84
BstC8I GCNNGC 2 cut(s) 22, 937
BstDEI CTNAG 6 cut(s) 7, 206, 614, 684, 798, 834
BstDSI CCRYGG 1 cut(s) 382
BstF5I GGATG 3 cut(s) 298, 460, 764
BstH2I RGCGCY 1 cut(s) 711
BstHHI GCGC 2 cut(s) 710, 988
BstKTI GATC 5 cut(s) 430, 505, 602, 1036, 1129
BstMAI GTCTC 1 cut(s) 236
BstMBI GATC 5 cut(s) 427, 502, 599, 1033, 1126
BstMWI GCNNNNNNNGC 5 cut(s) 17, 563, 662, 714, 750
BstNI CCWGG 1 cut(s) 705
BstNSI RCATGY 1 cut(s) 595
BstSCI CCNGG 2 cut(s) 236, 703
BstV1I GCAGC 3 cut(s) 294, 652, 740
BstV2I GAAGAC 2 cut(s) 93, 582
Bsu36I CCTNAGG 1 cut(s) 798
BsuRI GGCC 2 cut(s) 5, 744
BtgI CCRYGG 1 cut(s) 382
BtgZI GCGATG 1 cut(s) 875
BtsCI GGATG 3 cut(s) 298, 460, 764
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 2 cut(s) 111, 539
Cac8I GCNNGC 2 cut(s) 22, 937
CaiI CAGNNNCTG 1 cut(s) 710
CciI TCATGA 1 cut(s) 1036
CfoI GCGC 2 cut(s) 710, 988
Cfr10I RCCGGY 1 cut(s) 262
Cfr13I GGNCC 1 cut(s) 823
Csp6I GTAC 4 cut(s) 60, 260, 925, 1063
CspAI ACCGGT 1 cut(s) 262
CspCI CAANNNNNGTGG 2 cut(s) 364, 399
CviQI GTAC 4 cut(s) 60, 260, 925, 1063
DdeI CTNAG 6 cut(s) 7, 206, 614, 684, 798, 834
DpnI GATC 5 cut(s) 429, 504, 601, 1035, 1128
DpnII GATC 5 cut(s) 427, 502, 599, 1033, 1126
Eam1104I CTCTTC 1 cut(s) 965
EarI CTCTTC 1 cut(s) 965
Eco130I CCWWGG 1 cut(s) 400
Eco31I GGTCTC 1 cut(s) 236
Eco47I GGWCC 1 cut(s) 823
Eco57I CTGAAG 1 cut(s) 1136
Eco81I CCTNAGG 1 cut(s) 798
EcoRI GAATTC 1 cut(s) 219
EcoRII CCWGG 1 cut(s) 703
EcoT14I CCWWGG 1 cut(s) 400
ErhI CCWWGG 1 cut(s) 400
FbaI TGATCA 1 cut(s) 502
Fnu4HI GCNGC 4 cut(s) 308, 483, 666, 754
FokI GGATG 3 cut(s) 305, 467, 751
Fsp4HI GCNGC 4 cut(s) 308, 483, 666, 754
FspBI CTAG 2 cut(s) 63, 785
GlaI GCGC 2 cut(s) 709, 987
GluI GCNGC 4 cut(s) 308, 483, 666, 754
GsaI CCCAGC 1 cut(s) 482
GsuI CTGGAG 2 cut(s) 303, 903
HaeII RGCGCY 1 cut(s) 711
HaeIII GGCC 2 cut(s) 5, 744
HapII CCGG 3 cut(s) 237, 263, 431
HhaI GCGC 2 cut(s) 710, 988
Hin6I GCGC 2 cut(s) 708, 986
HinP1I GCGC 2 cut(s) 708, 986
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HindIII AAGCTT 2 cut(s) 518, 1049
HinfI GANTC 3 cut(s) 282, 326, 1057
HpaI GTTAAC 1 cut(s) 589
HpaII CCGG 3 cut(s) 237, 263, 431
HphI GGTGA 3 cut(s) 409, 639, 1112
Hpy166II GTNNAC 2 cut(s) 589, 826
Hpy188I TCNGA 4 cut(s) 574, 639, 837, 994
Hpy188III TCNNGA 5 cut(s) 320, 785, 908, 920, 1037
Hpy8I GTNNAC 2 cut(s) 589, 826
HpyCH4III ACNGT 2 cut(s) 181, 905
HpyCH4V TGCA 7 cut(s) 24, 137, 214, 499, 656, 717, 1085
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 563, 662, 714, 750
HpyF3I CTNAG 6 cut(s) 7, 206, 614, 684, 798, 834
HspAI GCGC 2 cut(s) 708, 986
KpnI GGTACC 1 cut(s) 263
Ksp22I TGATCA 1 cut(s) 502
KspAI GTTAAC 1 cut(s) 589
Kzo9I GATC 5 cut(s) 427, 502, 599, 1033, 1126
LmnI GCTCC 2 cut(s) 115, 701
Lsp1109I GCAGC 3 cut(s) 294, 652, 740
LweI GCATC 2 cut(s) 146, 973
MaeI CTAG 2 cut(s) 63, 785
MaeIII GTNAC 1 cut(s) 181
MalI GATC 5 cut(s) 429, 504, 601, 1035, 1128
MboI GATC 5 cut(s) 427, 502, 599, 1033, 1126
MboII GAAGA 6 cut(s) 98, 182, 260, 587, 952, 1135
MfeI CAATTG 1 cut(s) 621
MluCI AATT 5 cut(s) 219, 458, 621, 748, 760
MlyI GAGTC 1 cut(s) 276
MmeI TCCRAC 1 cut(s) 511
MseI TTAA 2 cut(s) 588, 758
MspA1I CMGCKG 2 cut(s) 227, 605
MspI CCGG 3 cut(s) 237, 263, 431
MspR9I CCNGG 2 cut(s) 238, 705
MunI CAATTG 1 cut(s) 621
Mva1269I GAATGC 1 cut(s) 335
MvaI CCWGG 1 cut(s) 705
MwoI GCNNNNNNNGC 5 cut(s) 17, 563, 662, 714, 750
NciI CCSGG 1 cut(s) 238
NdeII GATC 5 cut(s) 427, 502, 599, 1033, 1126
NlaIV GGNNCC 3 cut(s) 111, 261, 388
NspI RCATGY 1 cut(s) 595
NspV TTCGAA 1 cut(s) 84
PagI TCATGA 1 cut(s) 1036
PciI ACATGT 1 cut(s) 591
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PctI GAATGC 1 cut(s) 335
PfeI GAWTC 2 cut(s) 326, 1057
PinAI ACCGGT 1 cut(s) 262
PkrI GCNGC 4 cut(s) 309, 484, 667, 755
PleI GAGTC 1 cut(s) 276
PpsI GAGTC 1 cut(s) 276
PscI ACATGT 1 cut(s) 591
Psp6I CCWGG 1 cut(s) 703
PspFI CCCAGC 1 cut(s) 478
PspGI CCWGG 1 cut(s) 703
PspN4I GGNNCC 3 cut(s) 111, 261, 388
PspPI GGNCC 1 cut(s) 823
PstNI CAGNNNCTG 1 cut(s) 710
PvuII CAGCTG 2 cut(s) 227, 605
RsaI GTAC 4 cut(s) 61, 261, 926, 1064
RsaNI GTAC 4 cut(s) 60, 260, 925, 1063
SaqAI TTAA 2 cut(s) 588, 758
SatI GCNGC 4 cut(s) 308, 483, 666, 754
Sau3AI GATC 5 cut(s) 427, 502, 599, 1033, 1126
Sau96I GGNCC 1 cut(s) 823
SchI GAGTC 1 cut(s) 276
ScrFI CCNGG 2 cut(s) 238, 705
SfaNI GCATC 2 cut(s) 146, 973
SfuI TTCGAA 1 cut(s) 84
SinI GGWCC 1 cut(s) 823
Sse9I AATT 5 cut(s) 219, 458, 621, 748, 760
SsiI CCGC 2 cut(s) 483, 944
SspMI CTAG 2 cut(s) 63, 785
StyD4I CCNGG 2 cut(s) 236, 703
StyI CCWWGG 1 cut(s) 400
TaaI ACNGT 2 cut(s) 181, 905
TaqI TCGA 2 cut(s) 84, 867
TasI AATT 5 cut(s) 219, 458, 621, 748, 760
TatI WGTACW 2 cut(s) 924, 1062
TauI GCSGC 1 cut(s) 485
TfiI GAWTC 2 cut(s) 326, 1057
Tru1I TTAA 2 cut(s) 588, 758
Tru9I TTAA 2 cut(s) 588, 758
TscAI CASTG 2 cut(s) 111, 546
TseI GCWGC 3 cut(s) 307, 665, 753
TspDTI ATGAA 4 cut(s) 328, 471, 667, 753
TspRI CASTG 2 cut(s) 111, 546
VpaK11BI GGWCC 1 cut(s) 823
XapI RAATTY 2 cut(s) 219, 760
XbaI TCTAGA 1 cut(s) 784
XceI RCATGY 1 cut(s) 595
XspI CTAG 2 cut(s) 63, 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.