Belongs to the AB hydrolase superfamily. Lipase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 259 |
| AccB1I | GGYRCC | 1 cut(s) | 259 |
| AciI | CCGC | 2 cut(s) | 483, 1010 |
| AclWI | GGATC | 4 cut(s) | 422, 713, 883, 1187 |
| AcsI | RAATTY | 3 cut(s) | 219, 713, 826 |
| AcuI | CTGAAG | 1 cut(s) | 1202 |
| AfaI | GTAC | 4 cut(s) | 61, 261, 992, 1130 |
| AflII | CTTAAG | 1 cut(s) | 749 |
| AflIII | ACRYGT | 1 cut(s) | 591 |
| AgeI | ACCGGT | 1 cut(s) | 262 |
| AgsI | TTSAA | 1 cut(s) | 876 |
| AjnI | CCWGG | 1 cut(s) | 769 |
| AluBI | AGCT | 7 cut(s) | 73, 227, 520, 583, 605, 1078, 1117 |
| AluI | AGCT | 7 cut(s) | 73, 227, 520, 583, 605, 1078, 1117 |
| Alw26I | GTCTC | 1 cut(s) | 236 |
| AlwI | GGATC | 4 cut(s) | 422, 713, 883, 1187 |
| AoxI | GGCC | 1 cut(s) | 808 |
| ApeKI | GCWGC | 3 cut(s) | 307, 665, 819 |
| ApoI | RAATTY | 3 cut(s) | 219, 713, 826 |
| AsiGI | ACCGGT | 1 cut(s) | 262 |
| Asp718I | GGTACC | 1 cut(s) | 259 |
| AspLEI | GCGC | 1 cut(s) | 1054 |
| AspS9I | GGNCC | 1 cut(s) | 728 |
| AsuC2I | CCSGG | 2 cut(s) | 238, 250 |
| AsuHPI | GGTGA | 3 cut(s) | 409, 639, 1178 |
| AsuII | TTCGAA | 1 cut(s) | 84 |
| AvaII | GGWCC | 1 cut(s) | 728 |
| AxyI | CCTNAGG | 1 cut(s) | 864 |
| BanI | GGYRCC | 1 cut(s) | 259 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 252, 284 |
| BbsI | GAAGAC | 2 cut(s) | 93, 582 |
| BbvCI | CCTCAGC | 1 cut(s) | 684 |
| BbvI | GCAGC | 3 cut(s) | 294, 652, 806 |
| BccI | CCATC | 6 cut(s) | 119, 121, 188, 287, 908, 1100 |
| BciT130I | CCWGG | 1 cut(s) | 771 |
| BclI | TGATCA | 1 cut(s) | 502 |
| BcnI | CCSGG | 2 cut(s) | 238, 250 |
| BcoDI | GTCTC | 1 cut(s) | 236 |
| BfaI | CTAG | 3 cut(s) | 63, 584, 851 |
| BfmI | CTRYAG | 1 cut(s) | 27 |
| BfrI | CTTAAG | 1 cut(s) | 749 |
| BisI | GCNGC | 4 cut(s) | 308, 483, 666, 820 |
| BlsI | GCNGC | 4 cut(s) | 309, 484, 667, 821 |
| Bme1390I | CCNGG | 3 cut(s) | 238, 250, 771 |
| Bme18I | GGWCC | 1 cut(s) | 728 |
| BmgT120I | GGNCC | 1 cut(s) | 728 |
| BmiI | GGNNCC | 3 cut(s) | 111, 261, 388 |
| BmrFI | CCNGG | 3 cut(s) | 238, 250, 771 |
| BmrI | ACTGGG | 1 cut(s) | 551 |
| BmsI | GCATC | 2 cut(s) | 146, 1039 |
| BmuI | ACTGGG | 1 cut(s) | 551 |
| BpiI | GAAGAC | 2 cut(s) | 93, 582 |
| BpmI | CTGGAG | 2 cut(s) | 303, 969 |
| Bpu10I | CCTNAGC | 1 cut(s) | 684 |
| Bpu14I | TTCGAA | 1 cut(s) | 84 |
| BpuMI | CCSGG | 2 cut(s) | 238, 250 |
| BsaI | GGTCTC | 1 cut(s) | 236 |
| BsaJI | CCNNGG | 4 cut(s) | 237, 382, 400, 1030 |
| BsaWI | WCCGGW | 1 cut(s) | 262 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 267, 297 |
| Bse118I | RCCGGY | 1 cut(s) | 262 |
| Bse1I | ACTGG | 2 cut(s) | 490, 546 |
| Bse21I | CCTNAGG | 1 cut(s) | 864 |
| Bse3DI | GCAATG | 1 cut(s) | 678 |
| BseBI | CCWGG | 1 cut(s) | 771 |
| BseDI | CCNNGG | 4 cut(s) | 237, 382, 400, 1030 |
| BseGI | GGATG | 3 cut(s) | 298, 460, 830 |
| BseMI | GCAATG | 1 cut(s) | 678 |
| BseMII | CTCAG | 4 cut(s) | 220, 675, 878, 914 |
| BseNI | ACTGG | 2 cut(s) | 490, 546 |
| BseRI | GAGGAG | 1 cut(s) | 965 |
| BseXI | GCAGC | 3 cut(s) | 294, 652, 806 |
| BseYI | CCCAGC | 1 cut(s) | 478 |
| BsgI | GTGCAG | 1 cut(s) | 766 |
| BshFI | GGCC | 1 cut(s) | 810 |
| BshNI | GGYRCC | 1 cut(s) | 259 |
| BshTI | ACCGGT | 1 cut(s) | 262 |
| BsiSI | CCGG | 4 cut(s) | 237, 249, 263, 431 |
| BsmAI | GTCTC | 1 cut(s) | 236 |
| BsmI | GAATGC | 1 cut(s) | 335 |
| BsnI | GGCC | 1 cut(s) | 810 |
| Bso31I | GGTCTC | 1 cut(s) | 236 |
| Bsp119I | TTCGAA | 1 cut(s) | 84 |
| Bsp143I | GATC | 7 cut(s) | 427, 502, 599, 718, 888, 1099, 1192 |
| BspACI | CCGC | 2 cut(s) | 483, 1010 |
| BspANI | GGCC | 1 cut(s) | 810 |
| BspCNI | CTCAG | 4 cut(s) | 219, 676, 877, 913 |
| BspHI | TCATGA | 1 cut(s) | 1102 |
| BspLI | GGNNCC | 3 cut(s) | 111, 261, 388 |
| BspMAI | CTGCAG | 1 cut(s) | 31 |
| BspPI | GGATC | 4 cut(s) | 422, 713, 883, 1187 |
| BspT104I | TTCGAA | 1 cut(s) | 84 |
| BspT107I | GGYRCC | 1 cut(s) | 259 |
| BspTI | CTTAAG | 1 cut(s) | 749 |
| BspTNI | GGTCTC | 1 cut(s) | 236 |
| BsrDI | GCAATG | 1 cut(s) | 678 |
| BsrFI | RCCGGY | 1 cut(s) | 262 |
| BsrI | ACTGG | 2 cut(s) | 490, 546 |
| BssAI | RCCGGY | 1 cut(s) | 262 |
| BssECI | CCNNGG | 4 cut(s) | 237, 382, 400, 1030 |
| BssMI | GATC | 7 cut(s) | 427, 502, 599, 718, 888, 1099, 1192 |
| BssT1I | CCWWGG | 1 cut(s) | 400 |
| Bst2UI | CCWGG | 1 cut(s) | 771 |
| Bst4CI | ACNGT | 2 cut(s) | 181, 971 |
| Bst6I | CTCTTC | 1 cut(s) | 1031 |
| BstAFI | CTTAAG | 1 cut(s) | 749 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 662 |
| BstBI | TTCGAA | 1 cut(s) | 84 |
| BstC8I | GCNNGC | 1 cut(s) | 1003 |
| BstDEI | CTNAG | 5 cut(s) | 206, 614, 684, 864, 900 |
| BstDSI | CCRYGG | 1 cut(s) | 382 |
| BstF5I | GGATG | 3 cut(s) | 298, 460, 830 |
| BstHHI | GCGC | 1 cut(s) | 1054 |
| BstKTI | GATC | 7 cut(s) | 430, 505, 602, 721, 891, 1102, 1195 |
| BstMAI | GTCTC | 1 cut(s) | 236 |
| BstMBI | GATC | 7 cut(s) | 427, 502, 599, 718, 888, 1099, 1192 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 563, 662, 780, 816 |
| BstNI | CCWGG | 1 cut(s) | 771 |
| BstNSI | RCATGY | 1 cut(s) | 595 |
| BstSCI | CCNGG | 3 cut(s) | 236, 248, 769 |
| BstSFI | CTRYAG | 1 cut(s) | 27 |
| BstV1I | GCAGC | 3 cut(s) | 294, 652, 806 |
| BstV2I | GAAGAC | 2 cut(s) | 93, 582 |
| Bsu36I | CCTNAGG | 1 cut(s) | 864 |
| BsuRI | GGCC | 1 cut(s) | 810 |
| BtgI | CCRYGG | 1 cut(s) | 382 |
| BtgZI | GCGATG | 1 cut(s) | 941 |
| BtsCI | GGATG | 3 cut(s) | 298, 460, 830 |
| BtsI | GCAGTG | 1 cut(s) | 111 |
| BtsIMutI | CAGTG | 2 cut(s) | 111, 539 |
| Cac8I | GCNNGC | 1 cut(s) | 1003 |
| CciI | TCATGA | 1 cut(s) | 1102 |
| CfoI | GCGC | 1 cut(s) | 1054 |
| Cfr10I | RCCGGY | 1 cut(s) | 262 |
| Cfr13I | GGNCC | 1 cut(s) | 728 |
| Csp6I | GTAC | 4 cut(s) | 60, 260, 991, 1129 |
| CspAI | ACCGGT | 1 cut(s) | 262 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 364, 399 |
| CviQI | GTAC | 4 cut(s) | 60, 260, 991, 1129 |
| DdeI | CTNAG | 5 cut(s) | 206, 614, 684, 864, 900 |
| DpnI | GATC | 7 cut(s) | 429, 504, 601, 720, 890, 1101, 1194 |
| DpnII | GATC | 7 cut(s) | 427, 502, 599, 718, 888, 1099, 1192 |
| Eam1104I | CTCTTC | 1 cut(s) | 1031 |
| EarI | CTCTTC | 1 cut(s) | 1031 |
| Eco130I | CCWWGG | 1 cut(s) | 400 |
| Eco31I | GGTCTC | 1 cut(s) | 236 |
| Eco47I | GGWCC | 1 cut(s) | 728 |
| Eco57I | CTGAAG | 1 cut(s) | 1202 |
| Eco81I | CCTNAGG | 1 cut(s) | 864 |
| EcoRI | GAATTC | 1 cut(s) | 219 |
| EcoRII | CCWGG | 1 cut(s) | 769 |
| EcoT14I | CCWWGG | 1 cut(s) | 400 |
| ErhI | CCWWGG | 1 cut(s) | 400 |
| FbaI | TGATCA | 1 cut(s) | 502 |
| Fnu4HI | GCNGC | 4 cut(s) | 308, 483, 666, 820 |
| FokI | GGATG | 3 cut(s) | 305, 467, 817 |
| Fsp4HI | GCNGC | 4 cut(s) | 308, 483, 666, 820 |
| FspBI | CTAG | 3 cut(s) | 63, 584, 851 |
| GlaI | GCGC | 1 cut(s) | 1053 |
| GluI | GCNGC | 4 cut(s) | 308, 483, 666, 820 |
| GsaI | CCCAGC | 1 cut(s) | 482 |
| GsuI | CTGGAG | 2 cut(s) | 303, 969 |
| HaeIII | GGCC | 1 cut(s) | 810 |
| HapII | CCGG | 4 cut(s) | 237, 249, 263, 431 |
| HhaI | GCGC | 1 cut(s) | 1054 |
| Hin6I | GCGC | 1 cut(s) | 1052 |
| HinP1I | GCGC | 1 cut(s) | 1052 |
| HincII | GTYRAC | 2 cut(s) | 589, 778 |
| HindII | GTYRAC | 2 cut(s) | 589, 778 |
| HindIII | AAGCTT | 2 cut(s) | 518, 1115 |
| HinfI | GANTC | 3 cut(s) | 282, 326, 1123 |
| HpaI | GTTAAC | 1 cut(s) | 589 |
| HpaII | CCGG | 4 cut(s) | 237, 249, 263, 431 |
| HphI | GGTGA | 3 cut(s) | 409, 639, 1178 |
| Hpy166II | GTNNAC | 2 cut(s) | 589, 778 |
| Hpy188I | TCNGA | 4 cut(s) | 574, 639, 903, 1060 |
| Hpy188III | TCNNGA | 5 cut(s) | 320, 851, 974, 986, 1103 |
| Hpy8I | GTNNAC | 2 cut(s) | 589, 778 |
| HpyCH4III | ACNGT | 2 cut(s) | 181, 971 |
| HpyCH4V | TGCA | 7 cut(s) | 29, 137, 214, 499, 656, 783, 1151 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 563, 662, 780, 816 |
| HpyF3I | CTNAG | 5 cut(s) | 206, 614, 684, 864, 900 |
| HspAI | GCGC | 1 cut(s) | 1052 |
| KpnI | GGTACC | 1 cut(s) | 263 |
| Ksp22I | TGATCA | 1 cut(s) | 502 |
| KspAI | GTTAAC | 1 cut(s) | 589 |
| Kzo9I | GATC | 7 cut(s) | 427, 502, 599, 718, 888, 1099, 1192 |
| LmnI | GCTCC | 2 cut(s) | 115, 767 |
| Lsp1109I | GCAGC | 3 cut(s) | 294, 652, 806 |
| LweI | GCATC | 2 cut(s) | 146, 1039 |
| MaeI | CTAG | 3 cut(s) | 63, 584, 851 |
| MaeIII | GTNAC | 1 cut(s) | 181 |
| MalI | GATC | 7 cut(s) | 429, 504, 601, 720, 890, 1101, 1194 |
| MboI | GATC | 7 cut(s) | 427, 502, 599, 718, 888, 1099, 1192 |
| MboII | GAAGA | 5 cut(s) | 98, 182, 260, 587, 1018 |
| MfeI | CAATTG | 1 cut(s) | 621 |
| MluCI | AATT | 6 cut(s) | 219, 458, 621, 713, 814, 826 |
| MlyI | GAGTC | 1 cut(s) | 276 |
| MmeI | TCCRAC | 1 cut(s) | 511 |
| MseI | TTAA | 3 cut(s) | 588, 750, 824 |
| MspA1I | CMGCKG | 2 cut(s) | 227, 605 |
| MspCI | CTTAAG | 1 cut(s) | 749 |
| MspI | CCGG | 4 cut(s) | 237, 249, 263, 431 |
| MspR9I | CCNGG | 3 cut(s) | 238, 250, 771 |
| MunI | CAATTG | 1 cut(s) | 621 |
| Mva1269I | GAATGC | 1 cut(s) | 335 |
| MvaI | CCWGG | 1 cut(s) | 771 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 563, 662, 780, 816 |
| NciI | CCSGG | 2 cut(s) | 238, 250 |
| NdeII | GATC | 7 cut(s) | 427, 502, 599, 718, 888, 1099, 1192 |
| NlaIV | GGNNCC | 3 cut(s) | 111, 261, 388 |
| NspI | RCATGY | 1 cut(s) | 595 |
| NspV | TTCGAA | 1 cut(s) | 84 |
| PagI | TCATGA | 1 cut(s) | 1102 |
| PciI | ACATGT | 1 cut(s) | 591 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 90 |
| PctI | GAATGC | 1 cut(s) | 335 |
| PfeI | GAWTC | 2 cut(s) | 326, 1123 |
| PfoI | TCCNGGA | 1 cut(s) | 248 |
| PinAI | ACCGGT | 1 cut(s) | 262 |
| PkrI | GCNGC | 4 cut(s) | 309, 484, 667, 821 |
| PleI | GAGTC | 1 cut(s) | 276 |
| PpsI | GAGTC | 1 cut(s) | 276 |
| PscI | ACATGT | 1 cut(s) | 591 |
| Psp6I | CCWGG | 1 cut(s) | 769 |
| PspFI | CCCAGC | 1 cut(s) | 478 |
| PspGI | CCWGG | 1 cut(s) | 769 |
| PspN4I | GGNNCC | 3 cut(s) | 111, 261, 388 |
| PspPI | GGNCC | 1 cut(s) | 728 |
| PstI | CTGCAG | 1 cut(s) | 31 |
| PvuII | CAGCTG | 2 cut(s) | 227, 605 |
| RsaI | GTAC | 4 cut(s) | 61, 261, 992, 1130 |
| RsaNI | GTAC | 4 cut(s) | 60, 260, 991, 1129 |
| SaqAI | TTAA | 3 cut(s) | 588, 750, 824 |
| SatI | GCNGC | 4 cut(s) | 308, 483, 666, 820 |
| Sau3AI | GATC | 7 cut(s) | 427, 502, 599, 718, 888, 1099, 1192 |
| Sau96I | GGNCC | 1 cut(s) | 728 |
| SchI | GAGTC | 1 cut(s) | 276 |
| ScrFI | CCNGG | 3 cut(s) | 238, 250, 771 |
| SfaNI | GCATC | 2 cut(s) | 146, 1039 |
| SfcI | CTRYAG | 1 cut(s) | 27 |
| SfuI | TTCGAA | 1 cut(s) | 84 |
| SinI | GGWCC | 1 cut(s) | 728 |
| SmlI | CTYRAG | 1 cut(s) | 749 |
| SmoI | CTYRAG | 1 cut(s) | 749 |
| Sse9I | AATT | 6 cut(s) | 219, 458, 621, 713, 814, 826 |
| SsiI | CCGC | 2 cut(s) | 483, 1010 |
| SspMI | CTAG | 3 cut(s) | 63, 584, 851 |
| StyD4I | CCNGG | 3 cut(s) | 236, 248, 769 |
| StyI | CCWWGG | 1 cut(s) | 400 |
| TaaI | ACNGT | 2 cut(s) | 181, 971 |
| TaqI | TCGA | 2 cut(s) | 84, 933 |
| TasI | AATT | 6 cut(s) | 219, 458, 621, 713, 814, 826 |
| TatI | WGTACW | 2 cut(s) | 990, 1128 |
| TauI | GCSGC | 1 cut(s) | 485 |
| TfiI | GAWTC | 2 cut(s) | 326, 1123 |
| Tru1I | TTAA | 3 cut(s) | 588, 750, 824 |
| Tru9I | TTAA | 3 cut(s) | 588, 750, 824 |
| TscAI | CASTG | 2 cut(s) | 111, 546 |
| TseI | GCWGC | 3 cut(s) | 307, 665, 819 |
| TspDTI | ATGAA | 4 cut(s) | 328, 471, 667, 819 |
| TspRI | CASTG | 2 cut(s) | 111, 546 |
| Vha464I | CTTAAG | 1 cut(s) | 749 |
| VpaK11BI | GGWCC | 1 cut(s) | 728 |
| XapI | RAATTY | 3 cut(s) | 219, 713, 826 |
| XbaI | TCTAGA | 1 cut(s) | 850 |
| XceI | RCATGY | 1 cut(s) | 595 |
| XspI | CTAG | 3 cut(s) | 63, 584, 851 |