Belongs to the AB hydrolase superfamily. Lipase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 577 |
| AccII | CGCG | 1 cut(s) | 360 |
| AciI | CCGC | 3 cut(s) | 102, 293, 360 |
| AclWI | GGATC | 2 cut(s) | 306, 398 |
| AcsI | RAATTY | 2 cut(s) | 195, 493 |
| AcuI | CTGAAG | 1 cut(s) | 258 |
| AfaI | GTAC | 1 cut(s) | 583 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 207, 577 |
| AgsI | TTSAA | 1 cut(s) | 51 |
| AluBI | AGCT | 3 cut(s) | 76, 305, 440 |
| AluI | AGCT | 3 cut(s) | 76, 305, 440 |
| Alw26I | GTCTC | 2 cut(s) | 119, 215 |
| AlwI | GGATC | 2 cut(s) | 306, 398 |
| ApeKI | GCWGC | 3 cut(s) | 59, 76, 440 |
| ApoI | RAATTY | 2 cut(s) | 195, 493 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 317, 349 |
| AsuHPI | GGTGA | 2 cut(s) | 227, 521 |
| BanII | GRGCYC | 1 cut(s) | 261 |
| BbsI | GAAGAC | 1 cut(s) | 174 |
| BbvI | GCAGC | 3 cut(s) | 63, 71, 427 |
| BccI | CCATC | 2 cut(s) | 92, 92 |
| BcoDI | GTCTC | 2 cut(s) | 119, 215 |
| BfaI | CTAG | 1 cut(s) | 372 |
| BisI | GCNGC | 4 cut(s) | 60, 77, 103, 441 |
| BlsI | GCNGC | 4 cut(s) | 61, 78, 104, 442 |
| BmrI | ACTGGG | 1 cut(s) | 424 |
| BmsI | GCATC | 1 cut(s) | 259 |
| BmuI | ACTGGG | 1 cut(s) | 424 |
| BpiI | GAAGAC | 1 cut(s) | 174 |
| BsaJI | CCNNGG | 4 cut(s) | 181, 201, 516, 576 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 207, 577 |
| Bse1I | ACTGG | 2 cut(s) | 419, 425 |
| BseDI | CCNNGG | 4 cut(s) | 181, 201, 516, 576 |
| BseGI | GGATG | 3 cut(s) | 177, 274, 436 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 207, 577 |
| BseNI | ACTGG | 2 cut(s) | 419, 425 |
| BseXI | GCAGC | 3 cut(s) | 63, 71, 427 |
| Bsh1236I | CGCG | 1 cut(s) | 360 |
| BsiSI | CCGG | 3 cut(s) | 296, 407, 455 |
| BslFI | GGGAC | 3 cut(s) | 219, 376, 431 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 207, 577 |
| BsmAI | GTCTC | 2 cut(s) | 119, 215 |
| BsmFI | GGGAC | 3 cut(s) | 219, 376, 431 |
| BsmI | GAATGC | 1 cut(s) | 511 |
| Bsp1286I | GDGCHC | 1 cut(s) | 261 |
| Bsp143I | GATC | 3 cut(s) | 298, 403, 448 |
| Bsp19I | CCATGG | 1 cut(s) | 576 |
| BspACI | CCGC | 3 cut(s) | 102, 293, 360 |
| BspCNI | CTCAG | 1 cut(s) | 612 |
| BspFNI | CGCG | 1 cut(s) | 360 |
| BspPI | GGATC | 2 cut(s) | 306, 398 |
| BsrI | ACTGG | 2 cut(s) | 419, 425 |
| BssECI | CCNNGG | 4 cut(s) | 181, 201, 516, 576 |
| BssMI | GATC | 3 cut(s) | 298, 403, 448 |
| BssT1I | CCWWGG | 3 cut(s) | 201, 516, 576 |
| Bst4CI | ACNGT | 2 cut(s) | 173, 210 |
| Bst6I | CTCTTC | 1 cut(s) | 213 |
| BstC8I | GCNNGC | 2 cut(s) | 188, 348 |
| BstDEI | CTNAG | 2 cut(s) | 72, 599 |
| BstDSI | CCRYGG | 1 cut(s) | 576 |
| BstF5I | GGATG | 3 cut(s) | 177, 274, 436 |
| BstFNI | CGCG | 1 cut(s) | 360 |
| BstKTI | GATC | 3 cut(s) | 301, 406, 451 |
| BstMAI | GTCTC | 2 cut(s) | 119, 215 |
| BstMBI | GATC | 3 cut(s) | 298, 403, 448 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 108, 141 |
| BstUI | CGCG | 1 cut(s) | 360 |
| BstV1I | GCAGC | 3 cut(s) | 63, 71, 427 |
| BstV2I | GAAGAC | 1 cut(s) | 174 |
| BtgI | CCRYGG | 1 cut(s) | 576 |
| BtsCI | GGATG | 3 cut(s) | 177, 274, 436 |
| Cac8I | GCNNGC | 2 cut(s) | 188, 348 |
| CseI | GACGC | 1 cut(s) | 556 |
| Csp6I | GTAC | 1 cut(s) | 582 |
| CviAII | CATG | 4 cut(s) | 254, 279, 426, 577 |
| CviQI | GTAC | 1 cut(s) | 582 |
| DdeI | CTNAG | 2 cut(s) | 72, 599 |
| DpnI | GATC | 3 cut(s) | 300, 405, 450 |
| DpnII | GATC | 3 cut(s) | 298, 403, 448 |
| Eam1104I | CTCTTC | 1 cut(s) | 213 |
| EarI | CTCTTC | 1 cut(s) | 213 |
| EciI | GGCGGA | 1 cut(s) | 282 |
| Eco130I | CCWWGG | 3 cut(s) | 201, 516, 576 |
| Eco24I | GRGCYC | 1 cut(s) | 261 |
| Eco57I | CTGAAG | 1 cut(s) | 258 |
| EcoRI | GAATTC | 1 cut(s) | 195 |
| EcoT14I | CCWWGG | 3 cut(s) | 201, 516, 576 |
| EcoT38I | GRGCYC | 1 cut(s) | 261 |
| ErhI | CCWWGG | 3 cut(s) | 201, 516, 576 |
| FaeI | CATG | 4 cut(s) | 257, 282, 429, 580 |
| FaqI | GGGAC | 3 cut(s) | 219, 376, 431 |
| FatI | CATG | 4 cut(s) | 253, 278, 425, 576 |
| FauI | CCCGC | 1 cut(s) | 353 |
| Fnu4HI | GCNGC | 4 cut(s) | 60, 77, 103, 441 |
| FokI | GGATG | 3 cut(s) | 164, 281, 443 |
| FriOI | GRGCYC | 1 cut(s) | 261 |
| Fsp4HI | GCNGC | 4 cut(s) | 60, 77, 103, 441 |
| FspBI | CTAG | 1 cut(s) | 372 |
| GluI | GCNGC | 4 cut(s) | 60, 77, 103, 441 |
| HapII | CCGG | 3 cut(s) | 296, 407, 455 |
| HgaI | GACGC | 1 cut(s) | 556 |
| Hin1II | CATG | 4 cut(s) | 257, 282, 429, 580 |
| HindIII | AAGCTT | 1 cut(s) | 303 |
| HpaII | CCGG | 3 cut(s) | 296, 407, 455 |
| HphI | GGTGA | 2 cut(s) | 227, 521 |
| Hpy166II | GTNNAC | 5 cut(s) | 380, 475, 571, 584, 596 |
| Hpy8I | GTNNAC | 5 cut(s) | 380, 475, 571, 584, 596 |
| HpyAV | CCTTC | 1 cut(s) | 21 |
| HpyCH4III | ACNGT | 2 cut(s) | 173, 210 |
| HpyCH4V | TGCA | 4 cut(s) | 59, 79, 111, 190 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 108, 141 |
| HpyF3I | CTNAG | 2 cut(s) | 72, 599 |
| Hsp92II | CATG | 4 cut(s) | 257, 282, 429, 580 |
| Kzo9I | GATC | 3 cut(s) | 298, 403, 448 |
| LpnPI | CCDG | 9 cut(s) | 116, 198, 252, 309, 400, 406, 420, 468, 471 |
| Lsp1109I | GCAGC | 3 cut(s) | 63, 71, 427 |
| LweI | GCATC | 1 cut(s) | 259 |
| MaeI | CTAG | 1 cut(s) | 372 |
| MaeIII | GTNAC | 3 cut(s) | 157, 173, 246 |
| MalI | GATC | 3 cut(s) | 300, 405, 450 |
| MboI | GATC | 3 cut(s) | 298, 403, 448 |
| MboII | GAAGA | 3 cut(s) | 179, 230, 443 |
| MhlI | GDGCHC | 1 cut(s) | 261 |
| MluCI | AATT | 4 cut(s) | 195, 434, 493, 534 |
| MmeI | TCCRAC | 2 cut(s) | 102, 534 |
| MnlI | CCTC | 2 cut(s) | 191, 597 |
| MseI | TTAA | 3 cut(s) | 263, 464, 610 |
| MspI | CCGG | 3 cut(s) | 296, 407, 455 |
| Mva1269I | GAATGC | 1 cut(s) | 511 |
| MvnI | CGCG | 1 cut(s) | 360 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 108, 141 |
| NcoI | CCATGG | 1 cut(s) | 576 |
| NdeII | GATC | 3 cut(s) | 298, 403, 448 |
| NlaIII | CATG | 4 cut(s) | 257, 282, 429, 580 |
| NmeAIII | GCCGAG | 1 cut(s) | 162 |
| NmuCI | GTSAC | 1 cut(s) | 173 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 93 |
| PctI | GAATGC | 1 cut(s) | 511 |
| PflFI | GACNNNGTC | 1 cut(s) | 367 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 577 |
| PkrI | GCNGC | 4 cut(s) | 61, 78, 104, 442 |
| PsyI | GACNNNGTC | 1 cut(s) | 367 |
| RsaI | GTAC | 1 cut(s) | 583 |
| RsaNI | GTAC | 1 cut(s) | 582 |
| SaqAI | TTAA | 3 cut(s) | 263, 464, 610 |
| SatI | GCNGC | 4 cut(s) | 60, 77, 103, 441 |
| Sau3AI | GATC | 3 cut(s) | 298, 403, 448 |
| SduI | GDGCHC | 1 cut(s) | 261 |
| SetI | ASST | 5 cut(s) | 78, 307, 442, 534, 589 |
| SfaNI | GCATC | 1 cut(s) | 259 |
| Sse9I | AATT | 4 cut(s) | 195, 434, 493, 534 |
| SsiI | CCGC | 3 cut(s) | 102, 293, 360 |
| SspI | AATATT | 2 cut(s) | 526, 562 |
| SspMI | CTAG | 1 cut(s) | 372 |
| StyI | CCWWGG | 3 cut(s) | 201, 516, 576 |
| TaaI | ACNGT | 2 cut(s) | 173, 210 |
| TaqI | TCGA | 3 cut(s) | 14, 87, 96 |
| TasI | AATT | 4 cut(s) | 195, 434, 493, 534 |
| TauI | GCSGC | 1 cut(s) | 105 |
| Tru1I | TTAA | 3 cut(s) | 263, 464, 610 |
| Tru9I | TTAA | 3 cut(s) | 263, 464, 610 |
| TseFI | GTSAC | 1 cut(s) | 173 |
| TseI | GCWGC | 3 cut(s) | 59, 76, 440 |
| Tsp45I | GTSAC | 1 cut(s) | 173 |
| TspDTI | ATGAA | 1 cut(s) | 447 |
| Tth111I | GACNNNGTC | 1 cut(s) | 367 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 577 |
| XapI | RAATTY | 2 cut(s) | 195, 493 |
| XspI | CTAG | 1 cut(s) | 372 |