RLG00000022315
ERF Family

Belongs to the AB hydrolase superfamily. Lipase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000628
Physical Location & Seq
Forward (+)
82505605 .. 82508178
2574 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022315

Sequence Viewer

Length: 1221 bp
ATGGCTCTGCGCAGTTTAGCTTTTTGCCTGCTGATGGTTTTGGTTTCGGTGGTTGCACTTGAGCCTGGTGCGGTGGAGGGCTCGAGTCGGGGGCTTTTGGCTCAGGCAAATGCGGCTTTGGTGGCACAGGCGTGCGGTATATGCAAGGCCTTGGTGACTGTGCATGGTTACAAATGCCAAGAGATAACTGTGACTACCGACGATGGTTACATACTGAGCGTGCAGAGAATTGGTGGTGGTGGCGCCGGGAAACCACCGGTGATAATTCAGCATGGTATTCTTGCGGATGCAGCAGCCTGGCTGATGAACTCTCCGGATAAAAATCTGCCGTTAATTCTAGCTGATGGTGGATTTGATGTGTGGATGGCTAACACCAGAGGGACCAGATTTAGCCGGCGTCATACCTCCATGGACCCCAGCGATCCGAAATTTTGGAATTGGTCTTGGGATGAATTAGTGGCTCATGACCTACCAGCTGTTTTTAATTATGTGCACGGCCAAACAAAACAAAAGATTAACTATGTGGGTCATTCCCTGGGGACTTTGTTAGCATTGGCTTCGCTTTCAGAAGGGAAGCTAGTGGATCAGCTGAAATCAGTAGCTTTGCTAACTCCCATTGCTTACCTGACGCACATGAGTACACCAATTGGGGCCGCTGGGGCCAGACTCTTTGCTGGCGAGATCATAACAGCATTTCGCTTTGCAGAGTTCGATCCAAAAGGAGAGCCCGTGGCTGAATTCTTAGATCATCTATGTGCCACTCCAGGAGTTCCATGTTACGACATATTCTCAATACTCACCGGCAAAAATTGTTGTCTCAATGCTTCCACTGTTGATCTCTTCTTGGATCATCAGCCTCAGTCAACATCAACCAAGAACCTGGTGCACCTGTCTCAAGTTGTACGAAATGGCATTTTGACAAAATACGACCACGGGAGACCGTACACTAATTATATGCATTATGGCTCATTTTTCGCTCCGCATTATGATCTTTCTAAAATTCCCAACAACCTGCCCATGTTCATCACCTACGGTGGCCAAGATGCGCTCTCCGATGAACTTGACGTGTTGCATTTACTTGATCACTTGAAGTCTCACGACGCGGGAAAGCTTGTGGTTGATTTCATCCCCGAGTATGCCCATTTCGATTTTGTCATGGGGATCAATGCCAAGGACCTTGTGTATAATAAAGTGATTTCTTTCTTCAACCAGCAACAGTAA

Protein Analysis

407

Amino Acids

44.36

Weight (kDa)

6.07

Isoelectric Point (pI)

32.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydro_lipase PF04083 51 - 103 1.6e-16 Partial alpha/beta-hydrolase lipase region
Abhydrolase_1 PF00561 85 - 212 5.7e-11 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000628)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14180 AT5G14180 AT5G14180 AT5G14180 AT5G14180
fragaria_vesca FvH4_6g41960 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g53200
malus_domestica MD09G1013100.v1.1 MD09G1115500.v1.1 MD09G1115600.v1.1 MD09G1115800.v1.1 MD17G1013800.v1.1 MD17G1107400.v1.1
prunus_persica Prupe.3G208900_v2.0.a1 Prupe.3G208900_v2.0.a1 Prupe.3G304100_v2.0.a1
pyrus_communis pycom09g04000 pycom09g04020 pycom09g04040 pycom111g01090 pycom111g01110 pycom17g01090 pycom17g10010
rosa_chinensis RchiOBHm_Chr2g0157861 RchiOBHm_Chr2g0157881 RchiOBHm_Chr2g0175241 RchiOBHm_Chr2g0175251
rosa_laevigata RLG00000021032 RLG00000021033 RLG00000022314 RLG00000022315
rosa_multiflora Rmu_co8368271.1_g000001 Rmu_co8519319.1_g000001 Rmu_co8519319.1_g000002 Rmu_sc0002441.1_g000007 Rmu_sc0002441.1_g000008 Rmu_sc0010083.1_g000002 Rmu_sc0015795.1_g000001 Rmu_sc0015795.1_g000002
rosa_roxburghii Rroxscaffold_1G00036740 Rroxscaffold_2G00077450 Rroxscaffold_2G00077460 Rroxscaffold_2G00091880 Rroxscaffold_2G00091890 Rroxscaffold_2G00127210
rosa_rugosa Rorug02G0464200 Rorug02G0464200 Rorug02G0582000 Rorug02G0582100 Rorug02G0582100 Rorug02G0582100 Rorug02G0582200 Rorug02G0582200 Rorug02G0582200
rosa_samantha Rh2AG529400 Rh2AG529500 Rh2AG662400 Rh2AG662500 Rh2BG544000 Rh2BG544100 Rh2BG673600 Rh2BG673700 Rh2CG513700 Rh2CG513800 Rh2CG636800 Rh2DG552400
rosa_wichuraiana Rw2G043800 Rw2G043810 Rw2G054330 Rw2G054340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 11
Acc36I ACCTGC 1 cut(s) 1020
AccB1I GGYRCC 1 cut(s) 242
AccII CGCG 1 cut(s) 1103
AccIII TCCGGA 1 cut(s) 313
AciI CCGC 7 cut(s) 71, 113, 135, 284, 654, 980, 1103
AclWI GGATC 5 cut(s) 416, 591, 707, 855, 1169
AcoI YGGCCR 2 cut(s) 496, 1036
AcsI RAATTY 3 cut(s) 428, 737, 999
AcyI GRCGYC 2 cut(s) 243, 397
AfaI GTAC 3 cut(s) 640, 903, 944
AfiI CCNNNNNNNGG 1 cut(s) 34
AflIII ACRYGT 1 cut(s) 1065
AgeI ACCGGT 1 cut(s) 256
AgsI TTSAA 2 cut(s) 1090, 1207
AjiI CACGTC 1 cut(s) 1066
AjnI CCWGG 5 cut(s) 64, 296, 534, 763, 879
AleI CACNNNNGTG 1 cut(s) 130
AluBI AGCT 7 cut(s) 20, 341, 476, 577, 589, 602, 1111
AluI AGCT 7 cut(s) 20, 341, 476, 577, 589, 602, 1111
Alw21I GWGCWC 2 cut(s) 495, 888
Alw26I GTCTC 4 cut(s) 821, 897, 931, 1098
Alw44I GTGCAC 2 cut(s) 491, 884
AlwI GGATC 5 cut(s) 416, 591, 707, 855, 1169
Ama87I CYCGRG 2 cut(s) 82, 1130
Aor13HI TCCGGA 1 cut(s) 313
AoxI GGCC 5 cut(s) 147, 496, 651, 660, 1036
ApaLI GTGCAC 2 cut(s) 491, 884
ApeKI GCWGC 2 cut(s) 290, 293
ApoI RAATTY 3 cut(s) 428, 737, 999
AsiGI ACCGGT 1 cut(s) 256
AspLEI GCGC 3 cut(s) 12, 245, 1048
AspS9I GGNCC 5 cut(s) 381, 412, 651, 660, 1174
AsuC2I CCSGG 1 cut(s) 247
AsuHPI GGTGA 4 cut(s) 166, 271, 790, 1018
AvaI CYCGRG 2 cut(s) 82, 1130
AvaII GGWCC 3 cut(s) 381, 412, 1174
BaeGI GKGCMC 2 cut(s) 495, 888
BalI TGGCCA 1 cut(s) 1038
BanI GGYRCC 1 cut(s) 242
BanII GRGCYC 2 cut(s) 83, 729
Bbv12I GWGCWC 2 cut(s) 495, 888
BbvI GCAGC 2 cut(s) 302, 305
BccI CCATC 4 cut(s) 28, 197, 338, 358
BceAI ACGGC 2 cut(s) 313, 511
BcgI CGANNNNNNTGC 2 cut(s) 540, 574
BciT130I CCWGG 5 cut(s) 66, 298, 536, 765, 881
BclI TGATCA 1 cut(s) 1081
BcnI CCSGG 1 cut(s) 247
BcoDI GTCTC 4 cut(s) 821, 897, 931, 1098
BfaI CTAG 2 cut(s) 338, 578
BfoI RGCGCY 1 cut(s) 246
BfuAI ACCTGC 1 cut(s) 1020
BglI GCCNNNNNGGC 1 cut(s) 659
BisI GCNGC 4 cut(s) 114, 291, 294, 654
BlsI GCNGC 4 cut(s) 115, 292, 295, 655
Bme1390I CCNGG 6 cut(s) 66, 247, 298, 536, 765, 881
Bme18I GGWCC 3 cut(s) 381, 412, 1174
BmeT110I CYCGRG 2 cut(s) 82, 1130
BmgBI CACGTC 1 cut(s) 1066
BmgT120I GGNCC 5 cut(s) 381, 412, 651, 660, 1174
BmiI GGNNCC 5 cut(s) 244, 382, 414, 652, 661
BmrFI CCNGG 6 cut(s) 66, 247, 298, 536, 765, 881
BmsI GCATC 2 cut(s) 277, 1033
BpmI CTGGAG 1 cut(s) 747
Bpu10I CCTNAGC 1 cut(s) 102
BpuEI CTTGAG 2 cut(s) 80, 879
BpuMI CCSGG 1 cut(s) 247
BsaBI GATNNNNATC 1 cut(s) 321
BsaHI GRCGYC 2 cut(s) 243, 397
BsaI GGTCTC 1 cut(s) 931
BsaJI CCNNGG 7 cut(s) 150, 408, 534, 535, 729, 931, 1170
BsaWI WCCGGW 2 cut(s) 256, 313
BsaXI ACNNNNNCTCC 2 cut(s) 714, 744
Bsc4I CCNNNNNNNGG 1 cut(s) 34
Bse118I RCCGGY 3 cut(s) 256, 393, 800
Bse3DI GCAATG 1 cut(s) 615
Bse8I GATNNNNATC 1 cut(s) 321
BseAI TCCGGA 1 cut(s) 313
BseBI CCWGG 5 cut(s) 66, 298, 536, 765, 881
BseDI CCNNGG 7 cut(s) 150, 408, 534, 535, 729, 931, 1170
BseGI GGATG 4 cut(s) 292, 369, 454, 1125
BseJI GATNNNNATC 1 cut(s) 321
BseLI CCNNNNNNNGG 1 cut(s) 34
BseMI GCAATG 1 cut(s) 615
BseMII CTCAG 3 cut(s) 116, 206, 872
BseSI GKGCMC 2 cut(s) 495, 888
BseXI GCAGC 2 cut(s) 302, 305
BseYI CCCAGC 2 cut(s) 416, 656
BsgI GTGCAG 1 cut(s) 242
Bsh1236I CGCG 1 cut(s) 1103
BshFI GGCC 5 cut(s) 149, 498, 653, 662, 1038
BshNI GGYRCC 1 cut(s) 242
BshTI ACCGGT 1 cut(s) 256
BsiHKAI GWGCWC 2 cut(s) 495, 888
BsiHKCI CYCGRG 2 cut(s) 82, 1130
BsiSI CCGG 5 cut(s) 246, 257, 314, 394, 801
BslFI GGGAC 2 cut(s) 394, 553
BslI CCNNNNNNNGG 1 cut(s) 34
BsmAI GTCTC 4 cut(s) 821, 897, 931, 1098
BsmFI GGGAC 2 cut(s) 394, 553
BsnI GGCC 5 cut(s) 149, 498, 653, 662, 1038
Bso31I GGTCTC 1 cut(s) 931
BsoBI CYCGRG 2 cut(s) 82, 1130
Bsp1286I GDGCHC 4 cut(s) 83, 495, 729, 888
Bsp13I TCCGGA 1 cut(s) 313
Bsp19I CCATGG 1 cut(s) 408
BspACI CCGC 7 cut(s) 71, 113, 135, 284, 654, 980, 1103
BspANI GGCC 5 cut(s) 149, 498, 653, 662, 1038
BspCNI CTCAG 3 cut(s) 115, 207, 871
BspEI TCCGGA 1 cut(s) 313
BspFNI CGCG 1 cut(s) 1103
BspHI TCATGA 1 cut(s) 463
BspLI GGNNCC 5 cut(s) 244, 382, 414, 652, 661
BspMI ACCTGC 1 cut(s) 1020
BspPI GGATC 5 cut(s) 416, 591, 707, 855, 1169
BspT107I GGYRCC 1 cut(s) 242
BspTNI GGTCTC 1 cut(s) 931
BsrDI GCAATG 1 cut(s) 615
BsrFI RCCGGY 3 cut(s) 256, 393, 800
BssAI RCCGGY 3 cut(s) 256, 393, 800
BssECI CCNNGG 7 cut(s) 150, 408, 534, 535, 729, 931, 1170
BssNI GRCGYC 2 cut(s) 243, 397
BssT1I CCWWGG 3 cut(s) 150, 408, 1170
Bst2UI CCWGG 5 cut(s) 66, 298, 536, 765, 881
Bst4CI ACNGT 6 cut(s) 160, 190, 832, 942, 1034, 1218
Bst6I CTCTTC 1 cut(s) 845
BstACI GRCGYC 2 cut(s) 243, 397
BstC8I GCNNGC 5 cut(s) 29, 133, 221, 395, 676
BstDEI CTNAG 4 cut(s) 102, 215, 742, 858
BstDSI CCRYGG 3 cut(s) 408, 729, 931
BstF5I GGATG 4 cut(s) 292, 369, 454, 1125
BstFNI CGCG 1 cut(s) 1103
BstH2I RGCGCY 1 cut(s) 246
BstHHI GCGC 3 cut(s) 12, 245, 1048
BstMAI GTCTC 4 cut(s) 821, 897, 931, 1098
BstMWI GCNNNNNNNGC 5 cut(s) 113, 122, 141, 290, 659
BstNI CCWGG 5 cut(s) 66, 298, 536, 765, 881
BstSCI CCNGG 6 cut(s) 64, 245, 296, 534, 763, 879
BstSLI GKGCMC 2 cut(s) 495, 888
BstUI CGCG 1 cut(s) 1103
BstV1I GCAGC 2 cut(s) 302, 305
BstXI CCANNNNNNTGG 1 cut(s) 880
BsuRI GGCC 5 cut(s) 149, 498, 653, 662, 1038
BtgI CCRYGG 3 cut(s) 408, 729, 931
BtrI CACGTC 1 cut(s) 1066
BtsCI GGATG 4 cut(s) 292, 369, 454, 1125
BtsIMutI CAGTG 1 cut(s) 828
BveI ACCTGC 1 cut(s) 1020
Cac8I GCNNGC 5 cut(s) 29, 133, 221, 395, 676
CciI TCATGA 1 cut(s) 463
CfoI GCGC 3 cut(s) 12, 245, 1048
Cfr10I RCCGGY 3 cut(s) 256, 393, 800
Cfr13I GGNCC 5 cut(s) 381, 412, 651, 660, 1174
CseI GACGC 3 cut(s) 386, 637, 1109
CsiI ACCWGGT 1 cut(s) 879
Csp6I GTAC 3 cut(s) 639, 902, 943
CspAI ACCGGT 1 cut(s) 256
CviAII CATG 8 cut(s) 164, 272, 409, 464, 634, 774, 1018, 1156
CviQI GTAC 3 cut(s) 639, 902, 943
DdeI CTNAG 4 cut(s) 102, 215, 742, 858
DinI GGCGCC 1 cut(s) 244
EaeI YGGCCR 2 cut(s) 496, 1036
Eam1104I CTCTTC 1 cut(s) 845
EarI CTCTTC 1 cut(s) 845
Eco130I CCWWGG 3 cut(s) 150, 408, 1170
Eco147I AGGCCT 1 cut(s) 149
Eco24I GRGCYC 2 cut(s) 83, 729
Eco31I GGTCTC 1 cut(s) 931
Eco47I GGWCC 3 cut(s) 381, 412, 1174
Eco88I CYCGRG 2 cut(s) 82, 1130
EcoO109I RGGNCCY 1 cut(s) 1174
EcoRI GAATTC 1 cut(s) 737
EcoRII CCWGG 5 cut(s) 64, 296, 534, 763, 879
EcoT14I CCWWGG 3 cut(s) 150, 408, 1170
EcoT22I ATGCAT 1 cut(s) 960
EcoT38I GRGCYC 2 cut(s) 83, 729
EgeI GGCGCC 1 cut(s) 244
EheI GGCGCC 1 cut(s) 244
ErhI CCWWGG 3 cut(s) 150, 408, 1170
FaeI CATG 8 cut(s) 167, 275, 412, 467, 637, 777, 1021, 1159
FaqI GGGAC 2 cut(s) 394, 553
FatI CATG 8 cut(s) 163, 271, 408, 463, 633, 773, 1017, 1155
FauI CCCGC 1 cut(s) 1096
FbaI TGATCA 1 cut(s) 1081
Fnu4HI GCNGC 4 cut(s) 114, 291, 294, 654
FokI GGATG 4 cut(s) 299, 376, 461, 1112
FriOI GRGCYC 2 cut(s) 83, 729
Fsp4HI GCNGC 4 cut(s) 114, 291, 294, 654
FspBI CTAG 2 cut(s) 338, 578
FspI TGCGCA 1 cut(s) 11
GlaI GCGC 3 cut(s) 11, 244, 1047
GluI GCNGC 4 cut(s) 114, 291, 294, 654
GsaI CCCAGC 2 cut(s) 420, 660
GsuI CTGGAG 1 cut(s) 747
HaeII RGCGCY 1 cut(s) 246
HaeIII GGCC 5 cut(s) 149, 498, 653, 662, 1038
HapII CCGG 5 cut(s) 246, 257, 314, 394, 801
HgaI GACGC 3 cut(s) 386, 637, 1109
HhaI GCGC 3 cut(s) 12, 245, 1048
Hin1I GRCGYC 2 cut(s) 243, 397
Hin1II CATG 8 cut(s) 167, 275, 412, 467, 637, 777, 1021, 1159
Hin6I GCGC 3 cut(s) 10, 243, 1046
HinP1I GCGC 3 cut(s) 10, 243, 1046
HincII GTYRAC 1 cut(s) 864
HindII GTYRAC 1 cut(s) 864
HindIII AAGCTT 1 cut(s) 1109
HinfI GANTC 2 cut(s) 85, 666
HpaII CCGG 5 cut(s) 246, 257, 314, 394, 801
HphI GGTGA 4 cut(s) 166, 271, 790, 1018
Hpy166II GTNNAC 5 cut(s) 493, 641, 864, 886, 945
Hpy188I TCNGA 3 cut(s) 426, 568, 1054
Hpy188III TCNNGA 3 cut(s) 314, 464, 1097
Hpy8I GTNNAC 5 cut(s) 493, 641, 864, 886, 945
Hpy99I CGWCG 2 cut(s) 203, 1103
HpyAV CCTTC 1 cut(s) 563
HpyCH4III ACNGT 6 cut(s) 160, 190, 832, 942, 1034, 1218
HpyCH4IV ACGT 1 cut(s) 1065
HpyF10VI GCNNNNNNNGC 5 cut(s) 113, 122, 141, 290, 659
HpyF3I CTNAG 4 cut(s) 102, 215, 742, 858
HpySE526I ACGT 1 cut(s) 1065
Hsp92I GRCGYC 2 cut(s) 243, 397
Hsp92II CATG 8 cut(s) 167, 275, 412, 467, 637, 777, 1021, 1159
HspAI GCGC 3 cut(s) 10, 243, 1046
KasI GGCGCC 1 cut(s) 242
Kpn2I TCCGGA 1 cut(s) 313
KroI GCCGGC 1 cut(s) 393
KroNI GCCGGC 1 cut(s) 395
Ksp22I TGATCA 1 cut(s) 1081
LmnI GCTCC 1 cut(s) 982
Lsp1109I GCAGC 2 cut(s) 302, 305
LweI GCATC 2 cut(s) 277, 1033
MabI ACCWGGT 1 cut(s) 879
MaeI CTAG 2 cut(s) 338, 578
MaeII ACGT 1 cut(s) 1065
MaeIII GTNAC 5 cut(s) 154, 167, 190, 206, 776
MboII GAAGA 2 cut(s) 832, 1195
MfeI CAATTG 1 cut(s) 645
MhlI GDGCHC 4 cut(s) 83, 495, 729, 888
MlsI TGGCCA 1 cut(s) 1038
MluNI TGGCCA 1 cut(s) 1038
Mly113I GGCGCC 1 cut(s) 243
MlyI GAGTC 2 cut(s) 94, 660
MnlI CCTC 4 cut(s) 70, 371, 415, 867
Mox20I TGGCCA 1 cut(s) 1038
Mph1103I ATGCAT 1 cut(s) 960
MroI TCCGGA 1 cut(s) 313
MroNI GCCGGC 1 cut(s) 393
MscI TGGCCA 1 cut(s) 1038
MseI TTAA 3 cut(s) 332, 483, 516
MslI CAYNNNNRTG 2 cut(s) 130, 753
Msp20I TGGCCA 1 cut(s) 1038
MspA1I CMGCKG 3 cut(s) 476, 589, 656
MspI CCGG 5 cut(s) 246, 257, 314, 394, 801
MspR9I CCNGG 6 cut(s) 66, 247, 298, 536, 765, 881
MunI CAATTG 1 cut(s) 645
MvaI CCWGG 5 cut(s) 66, 298, 536, 765, 881
MvnI CGCG 1 cut(s) 1103
MwoI GCNNNNNNNGC 5 cut(s) 113, 122, 141, 290, 659
NaeI GCCGGC 1 cut(s) 395
NarI GGCGCC 1 cut(s) 243
NciI CCSGG 1 cut(s) 247
NcoI CCATGG 1 cut(s) 408
NgoMIV GCCGGC 1 cut(s) 393
NlaIII CATG 8 cut(s) 167, 275, 412, 467, 637, 777, 1021, 1159
NlaIV GGNNCC 5 cut(s) 244, 382, 414, 652, 661
NmuCI GTSAC 2 cut(s) 154, 190
NsbI TGCGCA 1 cut(s) 11
NsiI ATGCAT 1 cut(s) 960
OliI CACNNNNGTG 1 cut(s) 130
PaeR7I CTCGAG 1 cut(s) 82
PagI TCATGA 1 cut(s) 463
PasI CCCWGGG 1 cut(s) 535
PceI AGGCCT 1 cut(s) 149
PdiI GCCGGC 1 cut(s) 395
PfoI TCCNGGA 1 cut(s) 763
PinAI ACCGGT 1 cut(s) 256
PkrI GCNGC 4 cut(s) 115, 292, 295, 655
PleI GAGTC 2 cut(s) 93, 660
PluTI GGCGCC 1 cut(s) 246
PpsI GAGTC 2 cut(s) 93, 660
PpuMI RGGWCCY 1 cut(s) 1174
Psp5II RGGWCCY 1 cut(s) 1174
Psp6I CCWGG 5 cut(s) 64, 296, 534, 763, 879
PspFI CCCAGC 2 cut(s) 416, 656
PspGI CCWGG 5 cut(s) 64, 296, 534, 763, 879
PspN4I GGNNCC 5 cut(s) 244, 382, 414, 652, 661
PspPI GGNCC 5 cut(s) 381, 412, 651, 660, 1174
PspPPI RGGWCCY 1 cut(s) 1174
PspXI VCTCGAGB 1 cut(s) 82
PvuII CAGCTG 2 cut(s) 476, 589
RsaI GTAC 3 cut(s) 640, 903, 944
RsaNI GTAC 3 cut(s) 639, 902, 943
RseI CAYNNNNRTG 2 cut(s) 130, 753
SaqAI TTAA 3 cut(s) 332, 483, 516
SatI GCNGC 4 cut(s) 114, 291, 294, 654
Sau96I GGNCC 5 cut(s) 381, 412, 651, 660, 1174
SchI GAGTC 2 cut(s) 94, 660
ScrFI CCNGG 6 cut(s) 66, 247, 298, 536, 765, 881
SduI GDGCHC 4 cut(s) 83, 495, 729, 888
SexAI ACCWGGT 1 cut(s) 879
SfaNI GCATC 2 cut(s) 277, 1033
SfiI GGCCNNNNNGGCC 1 cut(s) 659
SfoI GGCGCC 1 cut(s) 244
Sfr274I CTCGAG 1 cut(s) 82
SgrAI CRCCGGYG 1 cut(s) 256
SinI GGWCC 3 cut(s) 381, 412, 1174
SlaI CTCGAG 1 cut(s) 82
SmiMI CAYNNNNRTG 2 cut(s) 130, 753
SmlI CTYRAG 3 cut(s) 59, 82, 894
SmoI CTYRAG 3 cut(s) 59, 82, 894
SseBI AGGCCT 1 cut(s) 149
SsiI CCGC 7 cut(s) 71, 113, 135, 284, 654, 980, 1103
SspDI GGCGCC 1 cut(s) 242
SspMI CTAG 2 cut(s) 338, 578
StuI AGGCCT 1 cut(s) 149
StyD4I CCNGG 6 cut(s) 64, 245, 296, 534, 763, 879
StyI CCWWGG 3 cut(s) 150, 408, 1170
TaaI ACNGT 6 cut(s) 160, 190, 832, 942, 1034, 1218
TaiI ACGT 1 cut(s) 1068
TaqI TCGA 3 cut(s) 83, 711, 1146
TatI WGTACW 1 cut(s) 638
TauI GCSGC 2 cut(s) 116, 656
Tru1I TTAA 3 cut(s) 332, 483, 516
Tru9I TTAA 3 cut(s) 332, 483, 516
TscAI CASTG 1 cut(s) 835
TseFI GTSAC 2 cut(s) 154, 190
TseI GCWGC 2 cut(s) 290, 293
Tsp45I GTSAC 2 cut(s) 154, 190
TspDTI ATGAA 5 cut(s) 320, 465, 1012, 1071, 1114
TspRI CASTG 1 cut(s) 835
VneI GTGCAC 2 cut(s) 491, 884
VpaK11BI GGWCC 3 cut(s) 381, 412, 1174
XapI RAATTY 3 cut(s) 428, 737, 999
XhoI CTCGAG 1 cut(s) 82
XspI CTAG 2 cut(s) 338, 578
Zsp2I ATGCAT 1 cut(s) 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.