RLG00000021033
ERF Family

Belongs to the AB hydrolase superfamily. Lipase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000628
Physical Location & Seq
Reverse (-)
71203912 .. 71205851
1940 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021033

Sequence Viewer

Length: 1233 bp
ATGAAGATGACGAACATTGGTACCCCTATCATTGTAGTTGTTCTGTTCTGGGCGTTGGCAGTTGGAACTCGAACAAAGCTGTTCTCTGAAGGAGATGGGATTGCTGTTTCGGCATTGGCTCCATCTGGTAGTGATGGCATTTGTGCATCAATGGTGGAAGCACAAGGCTATCCTTGTGAAGAACACACAGTGACAACAGAAGATGGCTATATACTCAGTATGCAGAGAATTCCAGGGGGTAAGTCTGGGAAGACATCAGGAAACAAGATACCGGTTCTTCTACAACATGGACTCCTCATGGATGGGATAACATGGCTGCTCCTACCTCCAGACCAAGCTTTGGCATTCCTCTTGGCGGATAATGGGTATGAGGTGTGGATTGCCAATACCCGTGGAACCCAATATAGCCTTGGCCATACATCACTCACACCCGATGATCCGGCTTATTGGGAATGGTCATGGGATGAATTGGTGGCTTATGACCTCTCGGCCACATACCAATATGTGCATAACCAAGCAGGACAGAAGATTCATTATGTTGGACATTCACTGGGAACTTTGATTGCTCTTGGTGCTTTTTCAAAAGACCAGCTAGTGAACATGTTGAGATCAGCTGTCTTACTTAGCCCAATTGCTCATGTTGGTCAGATGACCTCACCCCTTGCAAGAGCTGCTGCTGAAAACTTTATTGCTGAGGCTTTATACAAGGCAGGTTATAGAGAATTTAATCCACGATTAGAGGCAATAATCAAAGTTCTTAATGCATCATGTGCGAAATCAGGCGTTGACTGCACAAACCTCTTGACCTCTTTCACAGGCCAGAATTGCTGCTTAAATCCATCTATAGTACAAGTCTTTCTAGCTCATGAGCCTCAGGCATCTGCAACTAAAAACATGATCCATATCTCTCAGATGATAAGAGACGGAAAAGTTGCGATGTTTGACTACATTGACAAGGATGAGAACCAAAAACATTACGGGCAGGACACTCCTCCAGAGTACAAAATGGCAAGCATTCCGCATGACCTTCCTCTCTTCCTCAGTTACGGAGGGGCAGATGCGCTTTCTGATGTCCAAGATGTAAAGCTATTGCTCAACAGCCTCAAAGATCATGATGGAGATAAGCTTGTCGTTCAGTACAGAGATGATTATGCTCATGCTGATTTTGTTATGGGTGAAACTGCTAAGCGAGATGTTTATGATCCTCTCATGGCTTTCTTTAAACTTCAGTGA

Protein Analysis

411

Amino Acids

44.96

Weight (kDa)

5.24

Isoelectric Point (pI)

20.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydro_lipase PF04083 49 - 107 6.4e-22 Partial alpha/beta-hydrolase lipase region
Abhydrolase_1 PF00561 91 - 229 3.8e-10 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 92 - 232 3.4e-07 Alpha/beta hydrolase family
Hydrolase_4 PF12146 113 - 227 3.3e-06 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000628)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14180 AT5G14180 AT5G14180 AT5G14180 AT5G14180
fragaria_vesca FvH4_6g41960 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g53200
malus_domestica MD09G1013100.v1.1 MD09G1115500.v1.1 MD09G1115600.v1.1 MD09G1115800.v1.1 MD17G1013800.v1.1 MD17G1107400.v1.1
prunus_persica Prupe.3G208900_v2.0.a1 Prupe.3G208900_v2.0.a1 Prupe.3G304100_v2.0.a1
pyrus_communis pycom09g04000 pycom09g04020 pycom09g04040 pycom111g01090 pycom111g01110 pycom17g01090 pycom17g10010
rosa_chinensis RchiOBHm_Chr2g0157861 RchiOBHm_Chr2g0157881 RchiOBHm_Chr2g0175241 RchiOBHm_Chr2g0175251
rosa_laevigata RLG00000021032 RLG00000021033 RLG00000022314 RLG00000022315
rosa_multiflora Rmu_co8368271.1_g000001 Rmu_co8519319.1_g000001 Rmu_co8519319.1_g000002 Rmu_sc0002441.1_g000007 Rmu_sc0002441.1_g000008 Rmu_sc0010083.1_g000002 Rmu_sc0015795.1_g000001 Rmu_sc0015795.1_g000002
rosa_roxburghii Rroxscaffold_1G00036740 Rroxscaffold_2G00077450 Rroxscaffold_2G00077460 Rroxscaffold_2G00091880 Rroxscaffold_2G00091890 Rroxscaffold_2G00127210
rosa_rugosa Rorug02G0464200 Rorug02G0464200 Rorug02G0582000 Rorug02G0582100 Rorug02G0582100 Rorug02G0582100 Rorug02G0582200 Rorug02G0582200 Rorug02G0582200
rosa_samantha Rh2AG529400 Rh2AG529500 Rh2AG662400 Rh2AG662500 Rh2BG544000 Rh2BG544100 Rh2BG673600 Rh2BG673700 Rh2CG513700 Rh2CG513800 Rh2CG636800 Rh2DG552400
rosa_wichuraiana Rw2G043800 Rw2G043810 Rw2G054330 Rw2G054340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 701
Acc65I GGTACC 1 cut(s) 20
AccB1I GGYRCC 1 cut(s) 20
AccB7I CCANNNNNTGG 1 cut(s) 340
AciI CCGC 2 cut(s) 356, 1019
AclWI GGATC 3 cut(s) 431, 892, 1196
AcoI YGGCCR 2 cut(s) 412, 489
AcsI RAATTY 2 cut(s) 228, 722
AcuI CTGAAG 2 cut(s) 108, 1211
AdeI CACNNNGTG 1 cut(s) 190
AfaI GTAC 4 cut(s) 22, 849, 1001, 1139
AfiI CCNNNNNNNGG 2 cut(s) 340, 355
AflIII ACRYGT 1 cut(s) 600
AgeI ACCGGT 1 cut(s) 271
AgsI TTSAA 1 cut(s) 582
AjnI CCWGG 1 cut(s) 232
AluBI AGCT 8 cut(s) 79, 338, 592, 614, 671, 863, 1087, 1126
AluI AGCT 8 cut(s) 79, 338, 592, 614, 671, 863, 1087, 1126
Alw26I GTCTC 1 cut(s) 915
AlwI GGATC 3 cut(s) 431, 892, 1196
AoxI GGCC 3 cut(s) 412, 489, 817
ApeKI GCWGC 4 cut(s) 316, 671, 674, 828
ApoI RAATTY 2 cut(s) 228, 722
AsiGI ACCGGT 1 cut(s) 271
Asp718I GGTACC 1 cut(s) 20
AspLEI GCGC 1 cut(s) 1063
AsuHPI GGTGA 2 cut(s) 648, 1187
AxyI CCTNAGG 1 cut(s) 873
BaeI ACNNNNGTAYC 1 cut(s) 37
BalI TGGCCA 1 cut(s) 414
BanI GGYRCC 1 cut(s) 20
BbsI GAAGAC 1 cut(s) 257
BbvCI CCTCAGC 1 cut(s) 693
BbvI GCAGC 4 cut(s) 303, 658, 661, 815
BccI CCATC 7 cut(s) 89, 128, 130, 197, 296, 847, 1109
BciT130I CCWGG 1 cut(s) 234
BcoDI GTCTC 1 cut(s) 915
BfaI CTAG 2 cut(s) 593, 860
BfmI CTRYAG 1 cut(s) 843
BfuAI ACCTGC 1 cut(s) 701
BisI GCNGC 4 cut(s) 317, 672, 675, 829
BlpI GCTNAGC 1 cut(s) 1185
BlsI GCNGC 4 cut(s) 318, 673, 676, 830
Bme1390I CCNGG 1 cut(s) 234
BmiI GGNNCC 3 cut(s) 22, 120, 397
BmrFI CCNGG 1 cut(s) 234
BmrI ACTGGG 1 cut(s) 560
BmsI GCATC 4 cut(s) 155, 773, 887, 1048
BmuI ACTGGG 1 cut(s) 560
BpiI GAAGAC 1 cut(s) 257
BpmI CTGGAG 2 cut(s) 312, 978
Bpu10I CCTNAGC 1 cut(s) 693
Bpu1102I GCTNAGC 1 cut(s) 1185
BsaBI GATNNNNATC 1 cut(s) 902
BsaJI CCNNGG 3 cut(s) 233, 391, 409
BsaWI WCCGGW 1 cut(s) 271
BsaXI ACNNNNNCTCC 2 cut(s) 276, 306
Bsc4I CCNNNNNNNGG 2 cut(s) 340, 355
Bse118I RCCGGY 1 cut(s) 271
Bse1I ACTGG 1 cut(s) 555
Bse21I CCTNAGG 1 cut(s) 873
Bse8I GATNNNNATC 1 cut(s) 902
BseBI CCWGG 1 cut(s) 234
BseDI CCNNGG 3 cut(s) 233, 391, 409
BseGI GGATG 3 cut(s) 307, 469, 964
BseJI GATNNNNATC 1 cut(s) 902
BseLI CCNNNNNNNGG 2 cut(s) 340, 355
BseMII CTCAG 5 cut(s) 229, 684, 887, 923, 1054
BseNI ACTGG 1 cut(s) 555
BseRI GAGGAG 2 cut(s) 284, 981
BseXI GCAGC 4 cut(s) 303, 658, 661, 815
BsgI GTGCAG 1 cut(s) 775
BshFI GGCC 3 cut(s) 414, 491, 819
BshNI GGYRCC 1 cut(s) 20
BshTI ACCGGT 1 cut(s) 271
BsiSI CCGG 2 cut(s) 272, 440
BslI CCNNNNNNNGG 2 cut(s) 340, 355
BsmAI GTCTC 1 cut(s) 915
BsmBI CGTCTC 1 cut(s) 915
BsmI GAATGC 2 cut(s) 344, 1014
BsnI GGCC 3 cut(s) 414, 491, 819
Bsp143I GATC 5 cut(s) 436, 608, 897, 1108, 1201
Bsp1720I GCTNAGC 1 cut(s) 1185
BspACI CCGC 2 cut(s) 356, 1019
BspANI GGCC 3 cut(s) 414, 491, 819
BspCNI CTCAG 5 cut(s) 228, 685, 886, 922, 1053
BspHI TCATGA 2 cut(s) 865, 1111
BspLI GGNNCC 3 cut(s) 22, 120, 397
BspMI ACCTGC 1 cut(s) 701
BspPI GGATC 3 cut(s) 431, 892, 1196
BspT107I GGYRCC 1 cut(s) 20
BsrFI RCCGGY 1 cut(s) 271
BsrI ACTGG 1 cut(s) 555
BssAI RCCGGY 1 cut(s) 271
BssECI CCNNGG 3 cut(s) 233, 391, 409
BssMI GATC 5 cut(s) 436, 608, 897, 1108, 1201
BssT1I CCWWGG 1 cut(s) 409
Bst2UI CCWGG 1 cut(s) 234
Bst4CI ACNGT 1 cut(s) 190
Bst6I CTCTTC 1 cut(s) 1040
BstAPI GCANNNNNTGC 2 cut(s) 671, 770
BstC8I GCNNGC 1 cut(s) 1012
BstDEI CTNAG 7 cut(s) 215, 623, 693, 873, 909, 1040, 1185
BstDSI CCRYGG 1 cut(s) 391
BstF5I GGATG 3 cut(s) 307, 469, 964
BstHHI GCGC 1 cut(s) 1063
BstKTI GATC 5 cut(s) 439, 611, 900, 1111, 1204
BstMAI GTCTC 1 cut(s) 915
BstMBI GATC 5 cut(s) 436, 608, 897, 1108, 1201
BstMWI GCNNNNNNNGC 6 cut(s) 110, 572, 671, 770, 789, 825
BstNI CCWGG 1 cut(s) 234
BstNSI RCATGY 1 cut(s) 604
BstSCI CCNGG 1 cut(s) 232
BstSFI CTRYAG 1 cut(s) 843
BstV1I GCAGC 4 cut(s) 303, 658, 661, 815
BstV2I GAAGAC 1 cut(s) 257
Bsu36I CCTNAGG 1 cut(s) 873
BsuRI GGCC 3 cut(s) 414, 491, 819
BtgI CCRYGG 1 cut(s) 391
BtgZI GCGATG 1 cut(s) 950
BtsCI GGATG 3 cut(s) 307, 469, 964
BtsIMutI CAGTG 2 cut(s) 195, 548
BveI ACCTGC 1 cut(s) 701
Cac8I GCNNGC 1 cut(s) 1012
CciI TCATGA 2 cut(s) 865, 1111
CfoI GCGC 1 cut(s) 1063
Cfr10I RCCGGY 1 cut(s) 271
Csp6I GTAC 4 cut(s) 21, 848, 1000, 1138
CspAI ACCGGT 1 cut(s) 271
CspCI CAANNNNNGTGG 2 cut(s) 373, 408
CviQI GTAC 4 cut(s) 21, 848, 1000, 1138
DdeI CTNAG 7 cut(s) 215, 623, 693, 873, 909, 1040, 1185
DpnI GATC 5 cut(s) 438, 610, 899, 1110, 1203
DpnII GATC 5 cut(s) 436, 608, 897, 1108, 1201
DraI TTTAAA 1 cut(s) 1222
DraIII CACNNNGTG 1 cut(s) 190
EaeI YGGCCR 2 cut(s) 412, 489
Eam1104I CTCTTC 1 cut(s) 1040
EarI CTCTTC 1 cut(s) 1040
EciI GGCGGA 1 cut(s) 371
Eco130I CCWWGG 1 cut(s) 409
Eco57I CTGAAG 2 cut(s) 108, 1211
Eco81I CCTNAGG 1 cut(s) 873
EcoRI GAATTC 1 cut(s) 228
EcoRII CCWGG 1 cut(s) 232
EcoT14I CCWWGG 1 cut(s) 409
EcoT22I ATGCAT 1 cut(s) 766
ErhI CCWWGG 1 cut(s) 409
Esp3I CGTCTC 1 cut(s) 915
Fnu4HI GCNGC 4 cut(s) 317, 672, 675, 829
FokI GGATG 3 cut(s) 314, 476, 971
Fsp4HI GCNGC 4 cut(s) 317, 672, 675, 829
FspBI CTAG 2 cut(s) 593, 860
GlaI GCGC 1 cut(s) 1062
GluI GCNGC 4 cut(s) 317, 672, 675, 829
GsuI CTGGAG 2 cut(s) 312, 978
HaeIII GGCC 3 cut(s) 414, 491, 819
HapII CCGG 2 cut(s) 272, 440
HhaI GCGC 1 cut(s) 1063
Hin6I GCGC 1 cut(s) 1061
HinP1I GCGC 1 cut(s) 1061
HincII GTYRAC 1 cut(s) 787
HindII GTYRAC 1 cut(s) 787
HindIII AAGCTT 2 cut(s) 336, 1124
HinfI GANTC 2 cut(s) 291, 529
HpaII CCGG 2 cut(s) 272, 440
HphI GGTGA 2 cut(s) 648, 1187
Hpy166II GTNNAC 2 cut(s) 598, 787
Hpy188I TCNGA 4 cut(s) 88, 648, 912, 1069
Hpy188III TCNNGA 6 cut(s) 258, 329, 802, 866, 995, 1112
Hpy8I GTNNAC 2 cut(s) 598, 787
HpyAV CCTTC 2 cut(s) 83, 1037
HpyCH4III ACNGT 1 cut(s) 190
HpyCH4V TGCA 7 cut(s) 146, 223, 508, 665, 764, 792, 884
HpyF10VI GCNNNNNNNGC 6 cut(s) 110, 572, 671, 770, 789, 825
HpyF3I CTNAG 7 cut(s) 215, 623, 693, 873, 909, 1040, 1185
HspAI GCGC 1 cut(s) 1061
KpnI GGTACC 1 cut(s) 24
Kzo9I GATC 5 cut(s) 436, 608, 897, 1108, 1201
LmnI GCTCC 2 cut(s) 124, 324
Lsp1109I GCAGC 4 cut(s) 303, 658, 661, 815
LweI GCATC 4 cut(s) 155, 773, 887, 1048
MaeI CTAG 2 cut(s) 593, 860
MaeIII GTNAC 2 cut(s) 190, 1043
MalI GATC 5 cut(s) 438, 610, 899, 1110, 1203
MboI GATC 5 cut(s) 436, 608, 897, 1108, 1201
MboII GAAGA 7 cut(s) 16, 191, 212, 262, 269, 538, 1027
MfeI CAATTG 1 cut(s) 630
MlsI TGGCCA 1 cut(s) 414
MluCI AATT 5 cut(s) 228, 467, 630, 722, 823
MluNI TGGCCA 1 cut(s) 414
MlyI GAGTC 1 cut(s) 285
MmeI TCCRAC 2 cut(s) 43, 520
Mox20I TGGCCA 1 cut(s) 414
Mph1103I ATGCAT 1 cut(s) 766
MscI TGGCCA 1 cut(s) 414
MseI TTAA 4 cut(s) 726, 759, 833, 1221
Msp20I TGGCCA 1 cut(s) 414
MspA1I CMGCKG 1 cut(s) 614
MspI CCGG 2 cut(s) 272, 440
MspR9I CCNGG 1 cut(s) 234
MunI CAATTG 1 cut(s) 630
Mva1269I GAATGC 2 cut(s) 344, 1014
MvaI CCWGG 1 cut(s) 234
MwoI GCNNNNNNNGC 6 cut(s) 110, 572, 671, 770, 789, 825
NdeII GATC 5 cut(s) 436, 608, 897, 1108, 1201
NlaIV GGNNCC 3 cut(s) 22, 120, 397
NmeAIII GCCGAG 1 cut(s) 467
NmuCI GTSAC 1 cut(s) 190
NsiI ATGCAT 1 cut(s) 766
NspI RCATGY 1 cut(s) 604
PagI TCATGA 2 cut(s) 865, 1111
PciI ACATGT 1 cut(s) 600
PctI GAATGC 2 cut(s) 344, 1014
PfeI GAWTC 1 cut(s) 529
PflMI CCANNNNNTGG 1 cut(s) 340
PinAI ACCGGT 1 cut(s) 271
PkrI GCNGC 4 cut(s) 318, 673, 676, 830
PleI GAGTC 1 cut(s) 285
PpsI GAGTC 1 cut(s) 285
PscI ACATGT 1 cut(s) 600
Psp6I CCWGG 1 cut(s) 232
PspGI CCWGG 1 cut(s) 232
PspN4I GGNNCC 3 cut(s) 22, 120, 397
PvuII CAGCTG 1 cut(s) 614
RsaI GTAC 4 cut(s) 22, 849, 1001, 1139
RsaNI GTAC 4 cut(s) 21, 848, 1000, 1138
SaqAI TTAA 4 cut(s) 726, 759, 833, 1221
SatI GCNGC 4 cut(s) 317, 672, 675, 829
Sau3AI GATC 5 cut(s) 436, 608, 897, 1108, 1201
SchI GAGTC 1 cut(s) 285
ScrFI CCNGG 1 cut(s) 234
SfaNI GCATC 4 cut(s) 155, 773, 887, 1048
SfcI CTRYAG 1 cut(s) 843
Sse9I AATT 5 cut(s) 228, 467, 630, 722, 823
SsiI CCGC 2 cut(s) 356, 1019
SspMI CTAG 2 cut(s) 593, 860
StyD4I CCNGG 1 cut(s) 232
StyI CCWWGG 1 cut(s) 409
TaaI ACNGT 1 cut(s) 190
TaqI TCGA 1 cut(s) 70
TasI AATT 5 cut(s) 228, 467, 630, 722, 823
TatI WGTACW 3 cut(s) 847, 999, 1137
TfiI GAWTC 1 cut(s) 529
Tru1I TTAA 4 cut(s) 726, 759, 833, 1221
Tru9I TTAA 4 cut(s) 726, 759, 833, 1221
TscAI CASTG 2 cut(s) 195, 555
TseFI GTSAC 1 cut(s) 190
TseI GCWGC 4 cut(s) 316, 671, 674, 828
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 3 cut(s) 17, 480, 521
TspGWI ACGGA 2 cut(s) 939, 1062
TspRI CASTG 2 cut(s) 195, 555
Van91I CCANNNNNTGG 1 cut(s) 340
XapI RAATTY 2 cut(s) 228, 722
XceI RCATGY 1 cut(s) 604
XcmI CCANNNNNNNNNTGG 1 cut(s) 407
XspI CTAG 2 cut(s) 593, 860
Zsp2I ATGCAT 1 cut(s) 766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.