Rh2BG544000
ERF Family

Belongs to the AB hydrolase superfamily. Lipase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000628
Physical Location & Seq
Reverse (-)
75237959 .. 75240110
2152 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG544000.1

Sequence Viewer

Length: 918 bp
ATGGCAAACATTCTTACCTCTATTACTGCAGTTGTTCTATTCTGGGGATTGGCAGTTGGTACTAGAACAAGGCTATTCTCTTTCGAAGACAGCATGGTTTCAGCAGTGGCTCCATCTGGTAGTGATGGTATCTGTGCATCAATGGTGCTATCACAAGGCTACCCTTGTGAAGAACATACAGTAACAACACAAGATGGCTACATACTCAGTATGCAGAGAATTCCAGCTGGTAAGTCCGGGGAGACCTCCGGGAATAAGGTACCGGTTCTTCTACAACATGGACTCCTTATGGATGGGATAACATGGCTGCTACTACCTCCAGACGAATCTTTGGCATTCATCTTGGCAGATAATGGGTATGAGGTGTGGATTGCCAATACCCGTGGAACCAAATATAGCCTTGGACATACATCACTCACCCCCGATGATCCGGCTTATTGGGAGTGGTCATGGGATGAATTGGTCGCTTATGACCTCCCAGCCGCATACCAGTATGTGCATGATCAAGCAGGACAGAAGCTTCACTATGTTGGACATTCACTGGGAACTTTGATTGCTCTTGGTGCTTTTTCAGAAGACCAGCTAGTTAACATGTTGAGATCAGCTGTCTTACTTAGCCCAATTGCTCATGTTGGTCAGATAACCTCACCCCTTGCAAAAACTGCTGCTGAAAACTTCATTGCTGAGGATTTATACAAATCAGGTTTTAGAGAATTTGATCCAAAAGGACCAATGATAGTCCAAGTTCTTAAGGAAATCTGCTCCAAACCAGGCGTTGACTGCACAAACTTGATGACCTCTTTCACAGGCCAGAATTGCTGCTTAAATTCATCCATTACCGACATTTTTCTAGACCATGAGCCTCAGGCAACTTCAATAAAAAACATGATCCACCTCGCTCAGAGTAAGTGTTCCTAA

Protein Analysis

305

Amino Acids

33.17

Weight (kDa)

4.91

Isoelectric Point (pI)

27.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydro_lipase PF04083 47 - 104 7.7e-22 Partial alpha/beta-hydrolase lipase region
Abhydrolase_1 PF00561 88 - 214 3.8e-09 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000628)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14180 AT5G14180 AT5G14180 AT5G14180 AT5G14180
fragaria_vesca FvH4_6g41960 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g53200
malus_domestica MD09G1013100.v1.1 MD09G1115500.v1.1 MD09G1115600.v1.1 MD09G1115800.v1.1 MD17G1013800.v1.1 MD17G1107400.v1.1
prunus_persica Prupe.3G208900_v2.0.a1 Prupe.3G208900_v2.0.a1 Prupe.3G304100_v2.0.a1
pyrus_communis pycom09g04000 pycom09g04020 pycom09g04040 pycom111g01090 pycom111g01110 pycom17g01090 pycom17g10010
rosa_chinensis RchiOBHm_Chr2g0157861 RchiOBHm_Chr2g0157881 RchiOBHm_Chr2g0175241 RchiOBHm_Chr2g0175251
rosa_laevigata RLG00000021032 RLG00000021033 RLG00000022314 RLG00000022315
rosa_multiflora Rmu_co8368271.1_g000001 Rmu_co8519319.1_g000001 Rmu_co8519319.1_g000002 Rmu_sc0002441.1_g000007 Rmu_sc0002441.1_g000008 Rmu_sc0010083.1_g000002 Rmu_sc0015795.1_g000001 Rmu_sc0015795.1_g000002
rosa_roxburghii Rroxscaffold_1G00036740 Rroxscaffold_2G00077450 Rroxscaffold_2G00077460 Rroxscaffold_2G00091880 Rroxscaffold_2G00091890 Rroxscaffold_2G00127210
rosa_rugosa Rorug02G0464200 Rorug02G0464200 Rorug02G0582000 Rorug02G0582100 Rorug02G0582100 Rorug02G0582100 Rorug02G0582200 Rorug02G0582200 Rorug02G0582200
rosa_samantha Rh2AG529400 Rh2AG529500 Rh2AG662400 Rh2AG662500 Rh2BG544000 Rh2BG544100 Rh2BG673600 Rh2BG673700 Rh2CG513700 Rh2CG513800 Rh2CG636800 Rh2DG552400
rosa_wichuraiana Rw2G043800 Rw2G043810 Rw2G054330 Rw2G054340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 259
AccB1I GGYRCC 1 cut(s) 259
AciI CCGC 1 cut(s) 483
AclWI GGATC 3 cut(s) 422, 713, 883
AcsI RAATTY 3 cut(s) 219, 713, 826
AfaI GTAC 2 cut(s) 61, 261
AflII CTTAAG 1 cut(s) 749
AflIII ACRYGT 1 cut(s) 591
AgeI ACCGGT 1 cut(s) 262
AgsI TTSAA 1 cut(s) 876
AjnI CCWGG 1 cut(s) 769
AluBI AGCT 4 cut(s) 227, 520, 583, 605
AluI AGCT 4 cut(s) 227, 520, 583, 605
Alw26I GTCTC 1 cut(s) 236
AlwI GGATC 3 cut(s) 422, 713, 883
AoxI GGCC 1 cut(s) 808
ApeKI GCWGC 3 cut(s) 307, 665, 819
ApoI RAATTY 3 cut(s) 219, 713, 826
AsiGI ACCGGT 1 cut(s) 262
Asp718I GGTACC 1 cut(s) 259
AspS9I GGNCC 1 cut(s) 728
AsuC2I CCSGG 2 cut(s) 238, 250
AsuHPI GGTGA 2 cut(s) 409, 639
AsuII TTCGAA 1 cut(s) 84
AvaII GGWCC 1 cut(s) 728
AxyI CCTNAGG 1 cut(s) 864
BanI GGYRCC 1 cut(s) 259
BarI GAAGNNNNNNTAC 2 cut(s) 252, 284
BbsI GAAGAC 2 cut(s) 93, 582
BbvCI CCTCAGC 1 cut(s) 684
BbvI GCAGC 3 cut(s) 294, 652, 806
BccI CCATC 4 cut(s) 119, 121, 188, 287
BciT130I CCWGG 1 cut(s) 771
BclI TGATCA 1 cut(s) 502
BcnI CCSGG 2 cut(s) 238, 250
BcoDI GTCTC 1 cut(s) 236
BfaI CTAG 3 cut(s) 63, 584, 851
BfmI CTRYAG 1 cut(s) 27
BfrI CTTAAG 1 cut(s) 749
BisI GCNGC 4 cut(s) 308, 483, 666, 820
BlsI GCNGC 4 cut(s) 309, 484, 667, 821
Bme1390I CCNGG 3 cut(s) 238, 250, 771
Bme18I GGWCC 1 cut(s) 728
BmgT120I GGNCC 1 cut(s) 728
BmiI GGNNCC 3 cut(s) 111, 261, 388
BmrFI CCNGG 3 cut(s) 238, 250, 771
BmrI ACTGGG 1 cut(s) 551
BmsI GCATC 1 cut(s) 146
BmuI ACTGGG 1 cut(s) 551
BpiI GAAGAC 2 cut(s) 93, 582
BpmI CTGGAG 1 cut(s) 303
Bpu10I CCTNAGC 1 cut(s) 684
Bpu14I TTCGAA 1 cut(s) 84
BpuMI CCSGG 2 cut(s) 238, 250
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 3 cut(s) 237, 382, 400
BsaWI WCCGGW 1 cut(s) 262
BsaXI ACNNNNNCTCC 2 cut(s) 267, 297
Bse118I RCCGGY 1 cut(s) 262
Bse1I ACTGG 2 cut(s) 490, 546
Bse21I CCTNAGG 1 cut(s) 864
Bse3DI GCAATG 1 cut(s) 678
BseBI CCWGG 1 cut(s) 771
BseDI CCNNGG 3 cut(s) 237, 382, 400
BseGI GGATG 3 cut(s) 298, 460, 830
BseMI GCAATG 1 cut(s) 678
BseMII CTCAG 4 cut(s) 220, 675, 878, 914
BseNI ACTGG 2 cut(s) 490, 546
BseXI GCAGC 3 cut(s) 294, 652, 806
BseYI CCCAGC 1 cut(s) 478
BsgI GTGCAG 1 cut(s) 766
BshFI GGCC 1 cut(s) 810
BshNI GGYRCC 1 cut(s) 259
BshTI ACCGGT 1 cut(s) 262
BsiSI CCGG 4 cut(s) 237, 249, 263, 431
BsmAI GTCTC 1 cut(s) 236
BsmI GAATGC 1 cut(s) 335
BsnI GGCC 1 cut(s) 810
Bso31I GGTCTC 1 cut(s) 236
Bsp119I TTCGAA 1 cut(s) 84
Bsp143I GATC 5 cut(s) 427, 502, 599, 718, 888
BspACI CCGC 1 cut(s) 483
BspANI GGCC 1 cut(s) 810
BspCNI CTCAG 4 cut(s) 219, 676, 877, 913
BspLI GGNNCC 3 cut(s) 111, 261, 388
BspMAI CTGCAG 1 cut(s) 31
BspPI GGATC 3 cut(s) 422, 713, 883
BspT104I TTCGAA 1 cut(s) 84
BspT107I GGYRCC 1 cut(s) 259
BspTI CTTAAG 1 cut(s) 749
BspTNI GGTCTC 1 cut(s) 236
BsrDI GCAATG 1 cut(s) 678
BsrFI RCCGGY 1 cut(s) 262
BsrI ACTGG 2 cut(s) 490, 546
BssAI RCCGGY 1 cut(s) 262
BssECI CCNNGG 3 cut(s) 237, 382, 400
BssMI GATC 5 cut(s) 427, 502, 599, 718, 888
BssT1I CCWWGG 1 cut(s) 400
Bst2UI CCWGG 1 cut(s) 771
Bst4CI ACNGT 1 cut(s) 181
BstAFI CTTAAG 1 cut(s) 749
BstAPI GCANNNNNTGC 1 cut(s) 662
BstBI TTCGAA 1 cut(s) 84
BstDEI CTNAG 5 cut(s) 206, 614, 684, 864, 900
BstDSI CCRYGG 1 cut(s) 382
BstF5I GGATG 3 cut(s) 298, 460, 830
BstKTI GATC 5 cut(s) 430, 505, 602, 721, 891
BstMAI GTCTC 1 cut(s) 236
BstMBI GATC 5 cut(s) 427, 502, 599, 718, 888
BstMWI GCNNNNNNNGC 4 cut(s) 563, 662, 780, 816
BstNI CCWGG 1 cut(s) 771
BstNSI RCATGY 1 cut(s) 595
BstSCI CCNGG 3 cut(s) 236, 248, 769
BstSFI CTRYAG 1 cut(s) 27
BstV1I GCAGC 3 cut(s) 294, 652, 806
BstV2I GAAGAC 2 cut(s) 93, 582
Bsu36I CCTNAGG 1 cut(s) 864
BsuRI GGCC 1 cut(s) 810
BtgI CCRYGG 1 cut(s) 382
BtsCI GGATG 3 cut(s) 298, 460, 830
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 2 cut(s) 111, 539
Cfr10I RCCGGY 1 cut(s) 262
Cfr13I GGNCC 1 cut(s) 728
Csp6I GTAC 2 cut(s) 60, 260
CspAI ACCGGT 1 cut(s) 262
CspCI CAANNNNNGTGG 2 cut(s) 364, 399
CviAII CATG 9 cut(s) 94, 278, 303, 450, 500, 592, 629, 857, 886
CviQI GTAC 2 cut(s) 60, 260
DdeI CTNAG 5 cut(s) 206, 614, 684, 864, 900
DpnI GATC 5 cut(s) 429, 504, 601, 720, 890
DpnII GATC 5 cut(s) 427, 502, 599, 718, 888
Eco130I CCWWGG 1 cut(s) 400
Eco31I GGTCTC 1 cut(s) 236
Eco47I GGWCC 1 cut(s) 728
Eco81I CCTNAGG 1 cut(s) 864
EcoRI GAATTC 1 cut(s) 219
EcoRII CCWGG 1 cut(s) 769
EcoT14I CCWWGG 1 cut(s) 400
ErhI CCWWGG 1 cut(s) 400
FaeI CATG 9 cut(s) 97, 281, 306, 453, 503, 595, 632, 860, 889
FatI CATG 9 cut(s) 93, 277, 302, 449, 499, 591, 628, 856, 885
FbaI TGATCA 1 cut(s) 502
Fnu4HI GCNGC 4 cut(s) 308, 483, 666, 820
FokI GGATG 3 cut(s) 305, 467, 817
Fsp4HI GCNGC 4 cut(s) 308, 483, 666, 820
FspBI CTAG 3 cut(s) 63, 584, 851
GluI GCNGC 4 cut(s) 308, 483, 666, 820
GsaI CCCAGC 1 cut(s) 482
GsuI CTGGAG 1 cut(s) 303
HaeIII GGCC 1 cut(s) 810
HapII CCGG 4 cut(s) 237, 249, 263, 431
Hin1II CATG 9 cut(s) 97, 281, 306, 453, 503, 595, 632, 860, 889
HincII GTYRAC 2 cut(s) 589, 778
HindII GTYRAC 2 cut(s) 589, 778
HindIII AAGCTT 1 cut(s) 518
HinfI GANTC 2 cut(s) 282, 326
HpaI GTTAAC 1 cut(s) 589
HpaII CCGG 4 cut(s) 237, 249, 263, 431
HphI GGTGA 2 cut(s) 409, 639
Hpy166II GTNNAC 2 cut(s) 589, 778
Hpy188I TCNGA 3 cut(s) 574, 639, 903
Hpy188III TCNNGA 2 cut(s) 320, 851
Hpy8I GTNNAC 2 cut(s) 589, 778
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4V TGCA 6 cut(s) 29, 137, 214, 499, 656, 783
HpyF10VI GCNNNNNNNGC 4 cut(s) 563, 662, 780, 816
HpyF3I CTNAG 5 cut(s) 206, 614, 684, 864, 900
Hsp92II CATG 9 cut(s) 97, 281, 306, 453, 503, 595, 632, 860, 889
KpnI GGTACC 1 cut(s) 263
Ksp22I TGATCA 1 cut(s) 502
KspAI GTTAAC 1 cut(s) 589
Kzo9I GATC 5 cut(s) 427, 502, 599, 718, 888
LmnI GCTCC 2 cut(s) 115, 767
Lsp1109I GCAGC 3 cut(s) 294, 652, 806
LweI GCATC 1 cut(s) 146
MaeI CTAG 3 cut(s) 63, 584, 851
MaeIII GTNAC 1 cut(s) 181
MalI GATC 5 cut(s) 429, 504, 601, 720, 890
MboI GATC 5 cut(s) 427, 502, 599, 718, 888
MboII GAAGA 4 cut(s) 98, 182, 260, 587
MfeI CAATTG 1 cut(s) 621
MluCI AATT 6 cut(s) 219, 458, 621, 713, 814, 826
MlyI GAGTC 1 cut(s) 276
MmeI TCCRAC 1 cut(s) 511
MseI TTAA 3 cut(s) 588, 750, 824
MspA1I CMGCKG 2 cut(s) 227, 605
MspCI CTTAAG 1 cut(s) 749
MspI CCGG 4 cut(s) 237, 249, 263, 431
MspR9I CCNGG 3 cut(s) 238, 250, 771
MunI CAATTG 1 cut(s) 621
Mva1269I GAATGC 1 cut(s) 335
MvaI CCWGG 1 cut(s) 771
MwoI GCNNNNNNNGC 4 cut(s) 563, 662, 780, 816
NciI CCSGG 2 cut(s) 238, 250
NdeII GATC 5 cut(s) 427, 502, 599, 718, 888
NlaIII CATG 9 cut(s) 97, 281, 306, 453, 503, 595, 632, 860, 889
NlaIV GGNNCC 3 cut(s) 111, 261, 388
NspI RCATGY 1 cut(s) 595
NspV TTCGAA 1 cut(s) 84
PciI ACATGT 1 cut(s) 591
PctI GAATGC 1 cut(s) 335
PfeI GAWTC 1 cut(s) 326
PfoI TCCNGGA 1 cut(s) 248
PinAI ACCGGT 1 cut(s) 262
PkrI GCNGC 4 cut(s) 309, 484, 667, 821
PleI GAGTC 1 cut(s) 276
PpsI GAGTC 1 cut(s) 276
PscI ACATGT 1 cut(s) 591
Psp6I CCWGG 1 cut(s) 769
PspFI CCCAGC 1 cut(s) 478
PspGI CCWGG 1 cut(s) 769
PspN4I GGNNCC 3 cut(s) 111, 261, 388
PspPI GGNCC 1 cut(s) 728
PstI CTGCAG 1 cut(s) 31
PvuII CAGCTG 2 cut(s) 227, 605
RsaI GTAC 2 cut(s) 61, 261
RsaNI GTAC 2 cut(s) 60, 260
SaqAI TTAA 3 cut(s) 588, 750, 824
SatI GCNGC 4 cut(s) 308, 483, 666, 820
Sau3AI GATC 5 cut(s) 427, 502, 599, 718, 888
Sau96I GGNCC 1 cut(s) 728
SchI GAGTC 1 cut(s) 276
ScrFI CCNGG 3 cut(s) 238, 250, 771
SfaNI GCATC 1 cut(s) 146
SfcI CTRYAG 1 cut(s) 27
SfuI TTCGAA 1 cut(s) 84
SinI GGWCC 1 cut(s) 728
SmlI CTYRAG 1 cut(s) 749
SmoI CTYRAG 1 cut(s) 749
Sse9I AATT 6 cut(s) 219, 458, 621, 713, 814, 826
SsiI CCGC 1 cut(s) 483
SspMI CTAG 3 cut(s) 63, 584, 851
StyD4I CCNGG 3 cut(s) 236, 248, 769
StyI CCWWGG 1 cut(s) 400
TaaI ACNGT 1 cut(s) 181
TaqI TCGA 1 cut(s) 84
TasI AATT 6 cut(s) 219, 458, 621, 713, 814, 826
TauI GCSGC 1 cut(s) 485
TfiI GAWTC 1 cut(s) 326
Tru1I TTAA 3 cut(s) 588, 750, 824
Tru9I TTAA 3 cut(s) 588, 750, 824
TscAI CASTG 2 cut(s) 111, 546
TseI GCWGC 3 cut(s) 307, 665, 819
TspDTI ATGAA 4 cut(s) 328, 471, 667, 819
TspRI CASTG 2 cut(s) 111, 546
Vha464I CTTAAG 1 cut(s) 749
VpaK11BI GGWCC 1 cut(s) 728
XapI RAATTY 3 cut(s) 219, 713, 826
XbaI TCTAGA 1 cut(s) 850
XceI RCATGY 1 cut(s) 595
XspI CTAG 3 cut(s) 63, 584, 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.