pycom09g04000
ERF Family

Belongs to the AB hydrolase superfamily. Lipase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000628
Physical Location & Seq
Forward (+)
2975208 .. 2976016
809 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g04000.4

Sequence Viewer

Length: 489 bp
ATGGTGGCAACACAAGGCTATGCTTGCGAAGAACACACGGTGACAACACAAGATGGATATATTCTCAGCATGCAGAGAATTCCAGCGGGGAAGTCAGGGGGGCTGTCGGGGAAGAGGGTACCGGTACTTCTGCAACACGGTCTTCTGATGGATGGGATAACATGGGTGCTGCTACGTCCAGGCCAATCTTTGGCTTTCCTCTTAGCTGACAACGGGTATGAGGTGTGGATTGGAAACACCCGCGGAACCAAATACAGCCTCGGTCACACATCACTCAGTCCTGATGATCCGGCTTATTGGGAATGGTCATGGGATGAACTTGTCGCTTATGATCTCCCGGCTACTTTCCAGTATGTGCGAGATCAAACCAGGCAGAAAATTCATTACATTGGACATTCGCTGGTGCATTTCACAGACTCAGCAGCTTTCTTCTCTTCTGCAAACATTTTAAACAGTATATTAAACTTTTGGTGGAATTTTAAAGTATAA

Protein Analysis

163

Amino Acids

18.29

Weight (kDa)

6.02

Isoelectric Point (pI)

33.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000628)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14180 AT5G14180 AT5G14180 AT5G14180 AT5G14180
fragaria_vesca FvH4_6g41960 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g53200
malus_domestica MD09G1013100.v1.1 MD09G1115500.v1.1 MD09G1115600.v1.1 MD09G1115800.v1.1 MD17G1013800.v1.1 MD17G1107400.v1.1
prunus_persica Prupe.3G208900_v2.0.a1 Prupe.3G208900_v2.0.a1 Prupe.3G304100_v2.0.a1
pyrus_communis pycom09g04000 pycom09g04020 pycom09g04040 pycom111g01090 pycom111g01110 pycom17g01090 pycom17g10010
rosa_chinensis RchiOBHm_Chr2g0157861 RchiOBHm_Chr2g0157881 RchiOBHm_Chr2g0175241 RchiOBHm_Chr2g0175251
rosa_laevigata RLG00000021032 RLG00000021033 RLG00000022314 RLG00000022315
rosa_multiflora Rmu_co8368271.1_g000001 Rmu_co8519319.1_g000001 Rmu_co8519319.1_g000002 Rmu_sc0002441.1_g000007 Rmu_sc0002441.1_g000008 Rmu_sc0010083.1_g000002 Rmu_sc0015795.1_g000001 Rmu_sc0015795.1_g000002
rosa_roxburghii Rroxscaffold_1G00036740 Rroxscaffold_2G00077450 Rroxscaffold_2G00077460 Rroxscaffold_2G00091880 Rroxscaffold_2G00091890 Rroxscaffold_2G00127210
rosa_rugosa Rorug02G0464200 Rorug02G0464200 Rorug02G0582000 Rorug02G0582100 Rorug02G0582100 Rorug02G0582100 Rorug02G0582200 Rorug02G0582200 Rorug02G0582200
rosa_samantha Rh2AG529400 Rh2AG529500 Rh2AG662400 Rh2AG662500 Rh2BG544000 Rh2BG544100 Rh2BG673600 Rh2BG673700 Rh2CG513700 Rh2CG513800 Rh2CG636800 Rh2DG552400
rosa_wichuraiana Rw2G043800 Rw2G043810 Rw2G054330 Rw2G054340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 118
AccB1I GGYRCC 1 cut(s) 118
AccB7I CCANNNNNTGG 1 cut(s) 190
AccII CGCG 1 cut(s) 243
AciI CCGC 3 cut(s) 86, 241, 243
AclWI GGATC 1 cut(s) 281
AcsI RAATTY 3 cut(s) 78, 378, 475
AdeI CACNNNGTG 1 cut(s) 40
AfaI GTAC 2 cut(s) 120, 126
AfiI CCNNNNNNNGG 1 cut(s) 190
AgeI ACCGGT 1 cut(s) 121
AjnI CCWGG 2 cut(s) 178, 368
AluBI AGCT 2 cut(s) 206, 425
AluI AGCT 2 cut(s) 206, 425
AlwI GGATC 1 cut(s) 281
AoxI GGCC 1 cut(s) 181
ApeKI GCWGC 2 cut(s) 169, 422
ApoI RAATTY 3 cut(s) 78, 378, 475
AsiGI ACCGGT 1 cut(s) 121
Asp718I GGTACC 1 cut(s) 118
AsuC2I CCSGG 1 cut(s) 338
AsuHPI GGTGA 1 cut(s) 52
BanI GGYRCC 1 cut(s) 118
BarI GAAGNNNNNNTAC 2 cut(s) 111, 143
BbsI GAAGAC 1 cut(s) 134
BbvI GCAGC 2 cut(s) 156, 434
BccI CCATC 3 cut(s) 47, 142, 146
BciT130I CCWGG 2 cut(s) 180, 370
BcnI CCSGG 1 cut(s) 338
BisI GCNGC 2 cut(s) 170, 423
BlsI GCNGC 2 cut(s) 171, 424
Bme1390I CCNGG 3 cut(s) 180, 338, 370
BmiI GGNNCC 2 cut(s) 120, 247
BmrFI CCNGG 3 cut(s) 180, 338, 370
BpiI GAAGAC 1 cut(s) 134
BpuMI CCSGG 1 cut(s) 338
BsaJI CCNNGG 2 cut(s) 241, 259
BsaWI WCCGGW 1 cut(s) 121
Bsc4I CCNNNNNNNGG 1 cut(s) 190
Bse118I RCCGGY 1 cut(s) 121
Bse1I ACTGG 1 cut(s) 349
BseBI CCWGG 2 cut(s) 180, 370
BseDI CCNNGG 2 cut(s) 241, 259
BseGI GGATG 2 cut(s) 157, 319
BseLI CCNNNNNNNGG 1 cut(s) 190
BseMII CTCAG 3 cut(s) 79, 289, 432
BseNI ACTGG 1 cut(s) 349
BseXI GCAGC 2 cut(s) 156, 434
Bsh1236I CGCG 1 cut(s) 243
BshFI GGCC 1 cut(s) 183
BshNI GGYRCC 1 cut(s) 118
BshTI ACCGGT 1 cut(s) 121
BsiSI CCGG 3 cut(s) 122, 290, 338
BslI CCNNNNNNNGG 1 cut(s) 190
BsnI GGCC 1 cut(s) 183
Bsp143I GATC 3 cut(s) 286, 331, 361
BspACI CCGC 3 cut(s) 86, 241, 243
BspANI GGCC 1 cut(s) 183
BspCNI CTCAG 3 cut(s) 78, 288, 431
BspFNI CGCG 1 cut(s) 243
BspLI GGNNCC 2 cut(s) 120, 247
BspPI GGATC 1 cut(s) 281
BspT107I GGYRCC 1 cut(s) 118
BsrFI RCCGGY 1 cut(s) 121
BsrI ACTGG 1 cut(s) 349
BssAI RCCGGY 1 cut(s) 121
BssECI CCNNGG 2 cut(s) 241, 259
BssMI GATC 3 cut(s) 286, 331, 361
Bst2UI CCWGG 2 cut(s) 180, 370
Bst4CI ACNGT 3 cut(s) 40, 140, 455
Bst6I CTCTTC 2 cut(s) 107, 439
BstC8I GCNNGC 2 cut(s) 25, 71
BstDEI CTNAG 4 cut(s) 65, 202, 275, 418
BstDSI CCRYGG 1 cut(s) 241
BstF5I GGATG 2 cut(s) 157, 319
BstFNI CGCG 1 cut(s) 243
BstKTI GATC 3 cut(s) 289, 334, 364
BstMBI GATC 3 cut(s) 286, 331, 361
BstMWI GCNNNNNNNGC 1 cut(s) 24
BstNI CCWGG 2 cut(s) 180, 370
BstNSI RCATGY 1 cut(s) 73
BstSCI CCNGG 3 cut(s) 178, 336, 368
BstUI CGCG 1 cut(s) 243
BstV1I GCAGC 2 cut(s) 156, 434
BstV2I GAAGAC 1 cut(s) 134
BsuRI GGCC 1 cut(s) 183
BtgI CCRYGG 1 cut(s) 241
BtsCI GGATG 2 cut(s) 157, 319
Cac8I GCNNGC 2 cut(s) 25, 71
Cfr10I RCCGGY 1 cut(s) 121
Cfr42I CCGCGG 1 cut(s) 244
Csp6I GTAC 2 cut(s) 119, 125
CspAI ACCGGT 1 cut(s) 121
CviAII CATG 3 cut(s) 70, 162, 309
CviJI RGCY 9 cut(s) 18, 103, 183, 194, 206, 258, 293, 341, 425
CviKI_1 RGCY 9 cut(s) 18, 103, 183, 194, 206, 258, 293, 341, 425
CviQI GTAC 2 cut(s) 119, 125
DdeI CTNAG 4 cut(s) 65, 202, 275, 418
DpnI GATC 3 cut(s) 288, 333, 363
DpnII GATC 3 cut(s) 286, 331, 361
DraI TTTAAA 2 cut(s) 450, 481
DraIII CACNNNGTG 1 cut(s) 40
Eam1104I CTCTTC 2 cut(s) 107, 439
EarI CTCTTC 2 cut(s) 107, 439
EcoRI GAATTC 1 cut(s) 78
EcoRII CCWGG 2 cut(s) 178, 368
FaeI CATG 3 cut(s) 73, 165, 312
FatI CATG 3 cut(s) 69, 161, 308
FauI CCCGC 2 cut(s) 79, 248
Fnu4HI GCNGC 2 cut(s) 170, 423
FokI GGATG 2 cut(s) 164, 326
Fsp4HI GCNGC 2 cut(s) 170, 423
GluI GCNGC 2 cut(s) 170, 423
HaeIII GGCC 1 cut(s) 183
HapII CCGG 3 cut(s) 122, 290, 338
Hin1II CATG 3 cut(s) 73, 165, 312
HinfI GANTC 1 cut(s) 416
HpaII CCGG 3 cut(s) 122, 290, 338
HphI GGTGA 1 cut(s) 52
Hpy188I TCNGA 1 cut(s) 147
Hpy188III TCNNGA 1 cut(s) 281
HpyCH4III ACNGT 3 cut(s) 40, 140, 455
HpyCH4IV ACGT 1 cut(s) 175
HpyCH4V TGCA 4 cut(s) 73, 133, 406, 440
HpyF10VI GCNNNNNNNGC 1 cut(s) 24
HpyF3I CTNAG 4 cut(s) 65, 202, 275, 418
HpySE526I ACGT 1 cut(s) 175
Hsp92II CATG 3 cut(s) 73, 165, 312
KpnI GGTACC 1 cut(s) 122
KspI CCGCGG 1 cut(s) 244
Kzo9I GATC 3 cut(s) 286, 331, 361
Lsp1109I GCAGC 2 cut(s) 156, 434
MaeII ACGT 1 cut(s) 175
MaeIII GTNAC 2 cut(s) 40, 263
MalI GATC 3 cut(s) 288, 333, 363
MboI GATC 3 cut(s) 286, 331, 361
MboII GAAGA 5 cut(s) 41, 124, 134, 421, 426
MluCI AATT 3 cut(s) 78, 378, 475
MlyI GAGTC 1 cut(s) 410
MnlI CCTC 4 cut(s) 108, 209, 214, 269
MseI TTAA 3 cut(s) 449, 461, 480
MspA1I CMGCKG 2 cut(s) 86, 243
MspI CCGG 3 cut(s) 122, 290, 338
MspR9I CCNGG 3 cut(s) 180, 338, 370
MvaI CCWGG 2 cut(s) 180, 370
MvnI CGCG 1 cut(s) 243
MwoI GCNNNNNNNGC 1 cut(s) 24
NciI CCSGG 1 cut(s) 338
NdeII GATC 3 cut(s) 286, 331, 361
NlaIII CATG 3 cut(s) 73, 165, 312
NlaIV GGNNCC 2 cut(s) 120, 247
NmuCI GTSAC 2 cut(s) 40, 263
NspI RCATGY 1 cut(s) 73
PaeI GCATGC 1 cut(s) 73
PflMI CCANNNNNTGG 1 cut(s) 190
PinAI ACCGGT 1 cut(s) 121
PkrI GCNGC 2 cut(s) 171, 424
PleI GAGTC 1 cut(s) 410
PpsI GAGTC 1 cut(s) 410
Psp6I CCWGG 2 cut(s) 178, 368
PspGI CCWGG 2 cut(s) 178, 368
PspN4I GGNNCC 2 cut(s) 120, 247
RsaI GTAC 2 cut(s) 120, 126
RsaNI GTAC 2 cut(s) 119, 125
SacII CCGCGG 1 cut(s) 244
SaqAI TTAA 3 cut(s) 449, 461, 480
SatI GCNGC 2 cut(s) 170, 423
Sau3AI GATC 3 cut(s) 286, 331, 361
SchI GAGTC 1 cut(s) 410
ScrFI CCNGG 3 cut(s) 180, 338, 370
SetI ASST 4 cut(s) 178, 208, 225, 427
Sfr303I CCGCGG 1 cut(s) 244
SgrBI CCGCGG 1 cut(s) 244
SphI GCATGC 1 cut(s) 73
Sse9I AATT 3 cut(s) 78, 378, 475
SsiI CCGC 3 cut(s) 86, 241, 243
StyD4I CCNGG 3 cut(s) 178, 336, 368
TaaI ACNGT 3 cut(s) 40, 140, 455
TaiI ACGT 1 cut(s) 178
TaqII GACCGA 1 cut(s) 251
TasI AATT 3 cut(s) 78, 378, 475
Tru1I TTAA 3 cut(s) 449, 461, 480
Tru9I TTAA 3 cut(s) 449, 461, 480
TseFI GTSAC 2 cut(s) 40, 263
TseI GCWGC 2 cut(s) 169, 422
Tsp45I GTSAC 2 cut(s) 40, 263
TspDTI ATGAA 2 cut(s) 330, 371
Van91I CCANNNNNTGG 1 cut(s) 190
XapI RAATTY 3 cut(s) 78, 378, 475
XceI RCATGY 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.