Belongs to the AB hydrolase superfamily. Lipase family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 20 |
| AarI | CACCTGC | 1 cut(s) | 1210 |
| AatII | GACGTC | 1 cut(s) | 1095 |
| Acc36I | ACCTGC | 1 cut(s) | 1210 |
| Acc65I | GGTACC | 1 cut(s) | 433 |
| AccB1I | GGYRCC | 1 cut(s) | 433 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 505, 1262 |
| AccII | CGCG | 3 cut(s) | 558, 675, 1248 |
| AciI | CCGC | 4 cut(s) | 556, 558, 780, 864 |
| AclI | AACGTT | 1 cut(s) | 13 |
| AcsI | RAATTY | 6 cut(s) | 39, 95, 393, 693, 872, 1230 |
| AcuI | CTGAAG | 3 cut(s) | 782, 899, 1139 |
| AcyI | GRCGYC | 2 cut(s) | 419, 1092 |
| AdeI | CACNNNGTG | 1 cut(s) | 355 |
| AfaI | GTAC | 5 cut(s) | 131, 435, 441, 569, 1022 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 505, 1262, 1345, 1485 |
| AgeI | ACCGGT | 1 cut(s) | 935 |
| AgsI | TTSAA | 7 cut(s) | 82, 94, 1290, 1396, 1435, 1455, 1508 |
| AjnI | CCWGG | 5 cut(s) | 132, 493, 891, 898, 1255 |
| AluBI | AGCT | 9 cut(s) | 183, 248, 521, 656, 760, 839, 1108, 1147, 1286 |
| AluI | AGCT | 9 cut(s) | 183, 248, 521, 656, 760, 839, 1108, 1147, 1286 |
| Alw26I | GTCTC | 5 cut(s) | 39, 291, 942, 1158, 1505 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 842, 1266 |
| AoxI | GGCC | 3 cut(s) | 496, 861, 1409 |
| ApoI | RAATTY | 6 cut(s) | 39, 95, 393, 693, 872, 1230 |
| AsiGI | ACCGGT | 1 cut(s) | 935 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1353 |
| Asp718I | GGTACC | 1 cut(s) | 433 |
| AspLEI | GCGC | 3 cut(s) | 675, 1248, 1250 |
| AspS9I | GGNCC | 2 cut(s) | 1410, 1428 |
| AsuHPI | GGTGA | 4 cut(s) | 367, 813, 925, 1208 |
| AvaII | GGWCC | 1 cut(s) | 1428 |
| BanI | GGYRCC | 1 cut(s) | 433 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 426, 458 |
| BbsI | GAAGAC | 1 cut(s) | 449 |
| BbvCI | CCTCAGC | 1 cut(s) | 858 |
| BccI | CCATC | 3 cut(s) | 362, 457, 461 |
| BciT130I | CCWGG | 5 cut(s) | 134, 495, 893, 900, 1257 |
| BciVI | GTATCC | 2 cut(s) | 57, 365 |
| BcoDI | GTCTC | 5 cut(s) | 39, 291, 942, 1158, 1505 |
| BfmI | CTRYAG | 1 cut(s) | 1235 |
| BfuAI | ACCTGC | 1 cut(s) | 1210 |
| BfuI | GTATCC | 2 cut(s) | 57, 365 |
| BlpI | GCTNAGC | 1 cut(s) | 1206 |
| BmcAI | AGTACT | 1 cut(s) | 441 |
| Bme1390I | CCNGG | 5 cut(s) | 134, 495, 893, 900, 1257 |
| Bme18I | GGWCC | 1 cut(s) | 1428 |
| BmgT120I | GGNCC | 2 cut(s) | 1410, 1428 |
| BmiI | GGNNCC | 6 cut(s) | 112, 226, 282, 435, 562, 897 |
| BmrFI | CCNGG | 5 cut(s) | 134, 495, 893, 900, 1257 |
| BmrI | ACTGGG | 1 cut(s) | 285 |
| BmsI | GCATC | 4 cut(s) | 253, 804, 1069, 1324 |
| BmuI | ACTGGG | 1 cut(s) | 285 |
| BpiI | GAAGAC | 1 cut(s) | 449 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1485, 1517 |
| BpmI | CTGGAG | 2 cut(s) | 477, 999 |
| Bpu10I | CCTNAGC | 1 cut(s) | 858 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1206 |
| BpuEI | CTTGAG | 1 cut(s) | 790 |
| BsaHI | GRCGYC | 2 cut(s) | 419, 1092 |
| BsaJI | CCNNGG | 4 cut(s) | 556, 574, 805, 1424 |
| BsaWI | WCCGGW | 2 cut(s) | 683, 935 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 505, 1262, 1345, 1485 |
| Bse118I | RCCGGY | 1 cut(s) | 935 |
| Bse1I | ACTGG | 4 cut(s) | 291, 437, 664, 1016 |
| Bse3DI | GCAATG | 1 cut(s) | 969 |
| BseBI | CCWGG | 5 cut(s) | 134, 495, 893, 900, 1257 |
| BseDI | CCNNGG | 4 cut(s) | 556, 574, 805, 1424 |
| BseGI | GGATG | 3 cut(s) | 268, 472, 634 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 505, 1262, 1345, 1485 |
| BseMI | GCAATG | 1 cut(s) | 969 |
| BseMII | CTCAG | 4 cut(s) | 394, 604, 849, 1075 |
| BseNI | ACTGG | 4 cut(s) | 291, 437, 664, 1016 |
| BsePI | GCGCGC | 1 cut(s) | 1246 |
| BseRI | GAGGAG | 2 cut(s) | 1002, 1086 |
| BseYI | CCCAGC | 2 cut(s) | 652, 715 |
| BsgI | GTGCAG | 4 cut(s) | 278, 895, 1238, 1244 |
| Bsh1236I | CGCG | 3 cut(s) | 558, 675, 1248 |
| Bsh1285I | CGRYCG | 1 cut(s) | 106 |
| BshFI | GGCC | 3 cut(s) | 498, 863, 1411 |
| BshNI | GGYRCC | 1 cut(s) | 433 |
| BshTI | ACCGGT | 1 cut(s) | 935 |
| BsiEI | CGRYCG | 1 cut(s) | 106 |
| BsiSI | CCGG | 2 cut(s) | 684, 936 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 505, 1262, 1345, 1485 |
| BsmAI | GTCTC | 5 cut(s) | 39, 291, 942, 1158, 1505 |
| BsmBI | CGTCTC | 3 cut(s) | 291, 942, 1158 |
| BsmI | GAATGC | 2 cut(s) | 509, 1035 |
| BsnI | GGCC | 3 cut(s) | 498, 863, 1411 |
| Bsp1407I | TGTACA | 1 cut(s) | 1020 |
| Bsp143I | GATC | 4 cut(s) | 601, 646, 676, 773 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1206 |
| BspACI | CCGC | 4 cut(s) | 556, 558, 780, 864 |
| BspANI | GGCC | 3 cut(s) | 498, 863, 1411 |
| BspCNI | CTCAG | 4 cut(s) | 393, 603, 850, 1074 |
| BspFNI | CGCG | 3 cut(s) | 558, 675, 1248 |
| BspHI | TCATGA | 1 cut(s) | 1132 |
| BspLI | GGNNCC | 6 cut(s) | 112, 226, 282, 435, 562, 897 |
| BspMAI | CTGCAG | 1 cut(s) | 1239 |
| BspMI | ACCTGC | 1 cut(s) | 1210 |
| BspT107I | GGYRCC | 1 cut(s) | 433 |
| BsrDI | GCAATG | 1 cut(s) | 969 |
| BsrFI | RCCGGY | 1 cut(s) | 935 |
| BsrGI | TGTACA | 1 cut(s) | 1020 |
| BsrI | ACTGG | 4 cut(s) | 291, 437, 664, 1016 |
| BssAI | RCCGGY | 1 cut(s) | 935 |
| BssECI | CCNNGG | 4 cut(s) | 556, 574, 805, 1424 |
| BssHII | GCGCGC | 1 cut(s) | 1246 |
| BssMI | GATC | 4 cut(s) | 601, 646, 676, 773 |
| BssNI | GRCGYC | 2 cut(s) | 419, 1092 |
| BssT1I | CCWWGG | 1 cut(s) | 805 |
| Bst2UI | CCWGG | 5 cut(s) | 134, 495, 893, 900, 1257 |
| Bst4CI | ACNGT | 4 cut(s) | 104, 355, 455, 1363 |
| BstACI | GRCGYC | 2 cut(s) | 419, 1092 |
| BstAPI | GCANNNNNTGC | 4 cut(s) | 1216, 1234, 1243, 1266 |
| BstAUI | TGTACA | 1 cut(s) | 1020 |
| BstC8I | GCNNGC | 1 cut(s) | 1248 |
| BstDEI | CTNAG | 7 cut(s) | 380, 517, 590, 788, 858, 1061, 1206 |
| BstDSI | CCRYGG | 1 cut(s) | 556 |
| BstF5I | GGATG | 3 cut(s) | 268, 472, 634 |
| BstFNI | CGCG | 3 cut(s) | 558, 675, 1248 |
| BstHHI | GCGC | 3 cut(s) | 675, 1248, 1250 |
| BstKTI | GATC | 4 cut(s) | 604, 649, 679, 776 |
| BstMAI | GTCTC | 5 cut(s) | 39, 291, 942, 1158, 1505 |
| BstMBI | GATC | 4 cut(s) | 601, 646, 676, 773 |
| BstMCI | CGRYCG | 1 cut(s) | 106 |
| BstNI | CCWGG | 5 cut(s) | 134, 495, 893, 900, 1257 |
| BstNSI | RCATGY | 1 cut(s) | 757 |
| BstSCI | CCNGG | 5 cut(s) | 132, 493, 891, 898, 1255 |
| BstSFI | CTRYAG | 1 cut(s) | 1235 |
| BstUI | CGCG | 3 cut(s) | 558, 675, 1248 |
| BstV2I | GAAGAC | 1 cut(s) | 449 |
| BsuI | GTATCC | 2 cut(s) | 57, 365 |
| BsuRI | GGCC | 3 cut(s) | 498, 863, 1411 |
| BtgI | CCRYGG | 1 cut(s) | 556 |
| BtsCI | GGATG | 3 cut(s) | 268, 472, 634 |
| BtsI | GCAGTG | 1 cut(s) | 1267 |
| BtsIMutI | CAGTG | 5 cut(s) | 298, 1023, 1267, 1368, 1519 |
| BveI | ACCTGC | 1 cut(s) | 1210 |
| Cac8I | GCNNGC | 1 cut(s) | 1248 |
| CaiI | CAGNNNCTG | 2 cut(s) | 842, 1266 |
| CciI | TCATGA | 1 cut(s) | 1132 |
| CfoI | GCGC | 3 cut(s) | 675, 1248, 1250 |
| Cfr10I | RCCGGY | 1 cut(s) | 935 |
| Cfr13I | GGNCC | 2 cut(s) | 1410, 1428 |
| Cfr42I | CCGCGG | 1 cut(s) | 559 |
| CseI | GACGC | 1 cut(s) | 408 |
| Csp6I | GTAC | 5 cut(s) | 130, 434, 440, 568, 1021 |
| CspAI | ACCGGT | 1 cut(s) | 935 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 300, 335 |
| CviAII | CATG | 6 cut(s) | 58, 477, 754, 1133, 1178, 1192 |
| CviQI | GTAC | 5 cut(s) | 130, 434, 440, 568, 1021 |
| DdeI | CTNAG | 7 cut(s) | 380, 517, 590, 788, 858, 1061, 1206 |
| DpnI | GATC | 4 cut(s) | 603, 648, 678, 775 |
| DpnII | GATC | 4 cut(s) | 601, 646, 676, 773 |
| DraIII | CACNNNGTG | 1 cut(s) | 355 |
| Eco130I | CCWWGG | 1 cut(s) | 805 |
| Eco47I | GGWCC | 1 cut(s) | 1428 |
| Eco57I | CTGAAG | 3 cut(s) | 782, 899, 1139 |
| EcoRI | GAATTC | 1 cut(s) | 393 |
| EcoRII | CCWGG | 5 cut(s) | 132, 493, 891, 898, 1255 |
| EcoT14I | CCWWGG | 1 cut(s) | 805 |
| ErhI | CCWWGG | 1 cut(s) | 805 |
| Esp3I | CGTCTC | 3 cut(s) | 291, 942, 1158 |
| FaeI | CATG | 6 cut(s) | 61, 480, 757, 1136, 1181, 1195 |
| FatI | CATG | 6 cut(s) | 57, 476, 753, 1132, 1177, 1191 |
| FauI | CCCGC | 1 cut(s) | 563 |
| FokI | GGATG | 3 cut(s) | 275, 479, 641 |
| GlaI | GCGC | 3 cut(s) | 674, 1247, 1249 |
| GsaI | CCCAGC | 2 cut(s) | 656, 719 |
| GsuI | CTGGAG | 2 cut(s) | 477, 999 |
| HaeIII | GGCC | 3 cut(s) | 498, 863, 1411 |
| HapII | CCGG | 2 cut(s) | 684, 936 |
| HgaI | GACGC | 1 cut(s) | 408 |
| HhaI | GCGC | 3 cut(s) | 675, 1248, 1250 |
| Hin1I | GRCGYC | 2 cut(s) | 419, 1092 |
| Hin1II | CATG | 6 cut(s) | 61, 480, 757, 1136, 1181, 1195 |
| Hin6I | GCGC | 3 cut(s) | 673, 1246, 1248 |
| HinP1I | GCGC | 3 cut(s) | 673, 1246, 1248 |
| HincII | GTYRAC | 1 cut(s) | 907 |
| HindII | GTYRAC | 1 cut(s) | 907 |
| HindIII | AAGCTT | 1 cut(s) | 1145 |
| HinfI | GANTC | 4 cut(s) | 32, 212, 303, 1371 |
| HpaII | CCGG | 2 cut(s) | 684, 936 |
| HphI | GGTGA | 4 cut(s) | 367, 813, 925, 1208 |
| Hpy166II | GTNNAC | 3 cut(s) | 907, 933, 1021 |
| Hpy188I | TCNGA | 6 cut(s) | 462, 578, 813, 879, 1090, 1331 |
| Hpy188III | TCNNGA | 4 cut(s) | 36, 596, 1040, 1133 |
| Hpy8I | GTNNAC | 3 cut(s) | 907, 933, 1021 |
| Hpy99I | CGWCG | 3 cut(s) | 424, 641, 1094 |
| HpyAV | CCTTC | 4 cut(s) | 874, 1058, 1364, 1441 |
| HpyCH4III | ACNGT | 4 cut(s) | 104, 355, 455, 1363 |
| HpyCH4IV | ACGT | 2 cut(s) | 13, 1092 |
| HpyF3I | CTNAG | 7 cut(s) | 380, 517, 590, 788, 858, 1061, 1206 |
| HpySE526I | ACGT | 2 cut(s) | 13, 1092 |
| Hsp92I | GRCGYC | 2 cut(s) | 419, 1092 |
| Hsp92II | CATG | 6 cut(s) | 61, 480, 757, 1136, 1181, 1195 |
| HspAI | GCGC | 3 cut(s) | 673, 1246, 1248 |
| KpnI | GGTACC | 1 cut(s) | 437 |
| KspI | CCGCGG | 1 cut(s) | 559 |
| Kzo9I | GATC | 4 cut(s) | 601, 646, 676, 773 |
| LmnI | GCTCC | 3 cut(s) | 286, 1073, 1274 |
| LweI | GCATC | 4 cut(s) | 253, 804, 1069, 1324 |
| MaeII | ACGT | 2 cut(s) | 13, 1092 |
| MaeIII | GTNAC | 4 cut(s) | 355, 486, 1064, 1298 |
| MalI | GATC | 4 cut(s) | 603, 648, 678, 775 |
| MboI | GATC | 4 cut(s) | 601, 646, 676, 773 |
| MboII | GAAGA | 5 cut(s) | 169, 356, 449, 775, 1443 |
| MfeI | CAATTG | 1 cut(s) | 89 |
| MlyI | GAGTC | 2 cut(s) | 41, 221 |
| MmeI | TCCRAC | 4 cut(s) | 202, 685, 1113, 1296 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1353 |
| MseI | TTAA | 3 cut(s) | 179, 1367, 1389 |
| MslI | CAYNNNNRTG | 1 cut(s) | 938 |
| MspA1I | CMGCKG | 3 cut(s) | 558, 760, 839 |
| MspI | CCGG | 2 cut(s) | 684, 936 |
| MspR9I | CCNGG | 5 cut(s) | 134, 495, 893, 900, 1257 |
| MunI | CAATTG | 1 cut(s) | 89 |
| Mva1269I | GAATGC | 2 cut(s) | 509, 1035 |
| MvaI | CCWGG | 5 cut(s) | 134, 495, 893, 900, 1257 |
| MvnI | CGCG | 3 cut(s) | 558, 675, 1248 |
| NdeII | GATC | 4 cut(s) | 601, 646, 676, 773 |
| NlaIII | CATG | 6 cut(s) | 61, 480, 757, 1136, 1181, 1195 |
| NlaIV | GGNNCC | 6 cut(s) | 112, 226, 282, 435, 562, 897 |
| NmuCI | GTSAC | 2 cut(s) | 355, 1298 |
| NspI | RCATGY | 1 cut(s) | 757 |
| PagI | TCATGA | 1 cut(s) | 1132 |
| PaqCI | CACCTGC | 1 cut(s) | 1210 |
| PauI | GCGCGC | 1 cut(s) | 1246 |
| PctI | GAATGC | 2 cut(s) | 509, 1035 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1353 |
| PfeI | GAWTC | 2 cut(s) | 303, 1371 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 505, 1262 |
| PinAI | ACCGGT | 1 cut(s) | 935 |
| PleI | GAGTC | 2 cut(s) | 40, 220 |
| PpsI | GAGTC | 2 cut(s) | 40, 220 |
| PsiI | TTATAA | 1 cut(s) | 20 |
| Psp1406I | AACGTT | 1 cut(s) | 13 |
| Psp6I | CCWGG | 5 cut(s) | 132, 493, 891, 898, 1255 |
| PspFI | CCCAGC | 2 cut(s) | 652, 715 |
| PspGI | CCWGG | 5 cut(s) | 132, 493, 891, 898, 1255 |
| PspN4I | GGNNCC | 6 cut(s) | 112, 226, 282, 435, 562, 897 |
| PspPI | GGNCC | 2 cut(s) | 1410, 1428 |
| PstI | CTGCAG | 1 cut(s) | 1239 |
| PstNI | CAGNNNCTG | 2 cut(s) | 842, 1266 |
| PteI | GCGCGC | 1 cut(s) | 1246 |
| PvuII | CAGCTG | 2 cut(s) | 760, 839 |
| RsaI | GTAC | 5 cut(s) | 131, 435, 441, 569, 1022 |
| RsaNI | GTAC | 5 cut(s) | 130, 434, 440, 568, 1021 |
| RseI | CAYNNNNRTG | 1 cut(s) | 938 |
| SacII | CCGCGG | 1 cut(s) | 559 |
| SaqAI | TTAA | 3 cut(s) | 179, 1367, 1389 |
| Sau3AI | GATC | 4 cut(s) | 601, 646, 676, 773 |
| Sau96I | GGNCC | 2 cut(s) | 1410, 1428 |
| ScaI | AGTACT | 1 cut(s) | 441 |
| SchI | GAGTC | 2 cut(s) | 41, 221 |
| ScrFI | CCNGG | 5 cut(s) | 134, 495, 893, 900, 1257 |
| SfaNI | GCATC | 4 cut(s) | 253, 804, 1069, 1324 |
| SfcI | CTRYAG | 1 cut(s) | 1235 |
| Sfr303I | CCGCGG | 1 cut(s) | 559 |
| SgrBI | CCGCGG | 1 cut(s) | 559 |
| SinI | GGWCC | 1 cut(s) | 1428 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 938 |
| SmlI | CTYRAG | 1 cut(s) | 769 |
| SmoI | CTYRAG | 1 cut(s) | 769 |
| SsiI | CCGC | 4 cut(s) | 556, 558, 780, 864 |
| StyD4I | CCNGG | 5 cut(s) | 132, 493, 891, 898, 1255 |
| StyI | CCWWGG | 1 cut(s) | 805 |
| TaaI | ACNGT | 4 cut(s) | 104, 355, 455, 1363 |
| TaiI | ACGT | 2 cut(s) | 16, 1095 |
| TaqI | TCGA | 4 cut(s) | 37, 106, 870, 1125 |
| TaqII | GACCGA | 1 cut(s) | 1416 |
| TatI | WGTACW | 3 cut(s) | 439, 567, 1020 |
| TauI | GCSGC | 2 cut(s) | 782, 866 |
| TfiI | GAWTC | 2 cut(s) | 303, 1371 |
| Tru1I | TTAA | 3 cut(s) | 179, 1367, 1389 |
| Tru9I | TTAA | 3 cut(s) | 179, 1367, 1389 |
| TscAI | CASTG | 5 cut(s) | 298, 1023, 1267, 1368, 1519 |
| TseFI | GTSAC | 2 cut(s) | 355, 1298 |
| Tsp45I | GTSAC | 2 cut(s) | 355, 1298 |
| TspDTI | ATGAA | 4 cut(s) | 23, 170, 645, 1130 |
| TspGWI | ACGGA | 6 cut(s) | 205, 273, 315, 966, 1083, 1152 |
| TspRI | CASTG | 5 cut(s) | 298, 1023, 1267, 1368, 1519 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 505, 1262 |
| VpaK11BI | GGWCC | 1 cut(s) | 1428 |
| XapI | RAATTY | 6 cut(s) | 39, 95, 393, 693, 872, 1230 |
| XceI | RCATGY | 1 cut(s) | 757 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1353 |
| ZraI | GACGTC | 1 cut(s) | 1093 |
| ZrmI | AGTACT | 1 cut(s) | 441 |