RchiOBHm_Chr2g0157861
ERF Family

Belongs to the AB hydrolase superfamily. Lipase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000628
Physical Location & Seq
Reverse (-)
74217062 .. 74218618
1557 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52658

Sequence Viewer

Length: 744 bp
ATGGCCTTAGCTTTCTCTGCTTGCATTGGCGTTGTTCTATTCTGGGGATTGGCAGTTGGTACTAGAACAAAGCTATTCTCTTTCGAAGACAGCATGGTTTCAGCAGTGGCTCCATCTGGTAGTGATGGTATCTGTGCATCAATGGTGCTATCACAAGGCTACCCTTGTGAAGAACATACAGTAACAACACAAGATGGCTACATACTCAGTATGCAGAGAATTCCAGCTGGTAAGTCCGGGGAGACCTCCGGGAATAAGGTACCGGTTCTTCTACAACATGGACTCCTTATGGATGGGATAACATGGCTGCTACTACCTCCAGACGAATCTTTGGCATTCATCTTGGCAGATAATGGGTATGAGGTGTGGATTGCCAATACCCGTGGAACCAAATATAGCCTTGGACATACATCACTCACCCCCGATGATCCGGCTTATTGGGAGTGGTCATGGGATGAATTGGTCGCTTATGACCTCCCAGCCGCATACCAGTATGTGCATGATCAAGCAGGACAGAAGCTTCACTATGTTGGACATTCACTGGGAACTTTGATTGCTCTTGGTGCTTTTTCAGAAGACCAGCTAGTTAACATGTTGAGATCAGCTGTCTTACTTAGCCCAATTGCTCATGTTGGTCAGATAACCTCACCCCTTGCAAAAACTGCTGCTGAAAACTTCATTGCTGAGGTTAGAGTAATTCCTTCCATCTATATTAGCATATCCGGTAGAAACAATAGTCTTTGA

Protein Analysis

247

Amino Acids

26.65

Weight (kDa)

4.89

Isoelectric Point (pI)

26.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydro_lipase PF04083 47 - 104 5.4e-22 Partial alpha/beta-hydrolase lipase region
Abhydrolase_1 PF00561 88 - 209 2.9e-09 alpha/beta hydrolase fold
Hydrolase_4 PF12146 111 - 224 7.4e-06 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000628)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14180 AT5G14180 AT5G14180 AT5G14180 AT5G14180
fragaria_vesca FvH4_6g41960 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g53200
malus_domestica MD09G1013100.v1.1 MD09G1115500.v1.1 MD09G1115600.v1.1 MD09G1115800.v1.1 MD17G1013800.v1.1 MD17G1107400.v1.1
prunus_persica Prupe.3G208900_v2.0.a1 Prupe.3G208900_v2.0.a1 Prupe.3G304100_v2.0.a1
pyrus_communis pycom09g04000 pycom09g04020 pycom09g04040 pycom111g01090 pycom111g01110 pycom17g01090 pycom17g10010
rosa_chinensis RchiOBHm_Chr2g0157861 RchiOBHm_Chr2g0157881 RchiOBHm_Chr2g0175241 RchiOBHm_Chr2g0175251
rosa_laevigata RLG00000021032 RLG00000021033 RLG00000022314 RLG00000022315
rosa_multiflora Rmu_co8368271.1_g000001 Rmu_co8519319.1_g000001 Rmu_co8519319.1_g000002 Rmu_sc0002441.1_g000007 Rmu_sc0002441.1_g000008 Rmu_sc0010083.1_g000002 Rmu_sc0015795.1_g000001 Rmu_sc0015795.1_g000002
rosa_roxburghii Rroxscaffold_1G00036740 Rroxscaffold_2G00077450 Rroxscaffold_2G00077460 Rroxscaffold_2G00091880 Rroxscaffold_2G00091890 Rroxscaffold_2G00127210
rosa_rugosa Rorug02G0464200 Rorug02G0464200 Rorug02G0582000 Rorug02G0582100 Rorug02G0582100 Rorug02G0582100 Rorug02G0582200 Rorug02G0582200 Rorug02G0582200
rosa_samantha Rh2AG529400 Rh2AG529500 Rh2AG662400 Rh2AG662500 Rh2BG544000 Rh2BG544100 Rh2BG673600 Rh2BG673700 Rh2CG513700 Rh2CG513800 Rh2CG636800 Rh2DG552400
rosa_wichuraiana Rw2G043800 Rw2G043810 Rw2G054330 Rw2G054340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 259
AccB1I GGYRCC 1 cut(s) 259
AciI CCGC 1 cut(s) 483
AclWI GGATC 1 cut(s) 422
AcsI RAATTY 1 cut(s) 219
AfaI GTAC 2 cut(s) 61, 261
AflIII ACRYGT 1 cut(s) 591
AgeI ACCGGT 1 cut(s) 262
AluBI AGCT 6 cut(s) 11, 73, 227, 520, 583, 605
AluI AGCT 6 cut(s) 11, 73, 227, 520, 583, 605
Alw26I GTCTC 1 cut(s) 236
AlwI GGATC 1 cut(s) 422
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 307, 665
ApoI RAATTY 1 cut(s) 219
AsiGI ACCGGT 1 cut(s) 262
Asp718I GGTACC 1 cut(s) 259
AsuC2I CCSGG 2 cut(s) 238, 250
AsuHPI GGTGA 2 cut(s) 409, 639
AsuII TTCGAA 1 cut(s) 84
BanI GGYRCC 1 cut(s) 259
BarI GAAGNNNNNNTAC 2 cut(s) 252, 284
BbsI GAAGAC 2 cut(s) 93, 582
BbvCI CCTCAGC 1 cut(s) 684
BbvI GCAGC 2 cut(s) 294, 652
BccI CCATC 5 cut(s) 119, 121, 188, 287, 713
BclI TGATCA 1 cut(s) 502
BcnI CCSGG 2 cut(s) 238, 250
BcoDI GTCTC 1 cut(s) 236
BfaI CTAG 2 cut(s) 63, 584
BisI GCNGC 3 cut(s) 308, 483, 666
BlsI GCNGC 3 cut(s) 309, 484, 667
Bme1390I CCNGG 2 cut(s) 238, 250
BmiI GGNNCC 3 cut(s) 111, 261, 388
BmrFI CCNGG 2 cut(s) 238, 250
BmrI ACTGGG 1 cut(s) 551
BmsI GCATC 1 cut(s) 146
BmuI ACTGGG 1 cut(s) 551
BpiI GAAGAC 2 cut(s) 93, 582
BpmI CTGGAG 1 cut(s) 303
Bpu10I CCTNAGC 2 cut(s) 7, 684
Bpu14I TTCGAA 1 cut(s) 84
BpuMI CCSGG 2 cut(s) 238, 250
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 3 cut(s) 237, 382, 400
BsaWI WCCGGW 2 cut(s) 262, 722
BsaXI ACNNNNNCTCC 2 cut(s) 267, 297
Bse118I RCCGGY 1 cut(s) 262
Bse1I ACTGG 2 cut(s) 490, 546
Bse3DI GCAATG 1 cut(s) 678
BseDI CCNNGG 3 cut(s) 237, 382, 400
BseGI GGATG 2 cut(s) 298, 460
BseMI GCAATG 1 cut(s) 678
BseMII CTCAG 2 cut(s) 220, 675
BseNI ACTGG 2 cut(s) 490, 546
BseXI GCAGC 2 cut(s) 294, 652
BseYI CCCAGC 1 cut(s) 478
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 259
BshTI ACCGGT 1 cut(s) 262
BsiSI CCGG 5 cut(s) 237, 249, 263, 431, 723
BsmAI GTCTC 1 cut(s) 236
BsmI GAATGC 1 cut(s) 335
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 236
Bsp119I TTCGAA 1 cut(s) 84
Bsp143I GATC 3 cut(s) 427, 502, 599
BspACI CCGC 1 cut(s) 483
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 219, 676
BspLI GGNNCC 3 cut(s) 111, 261, 388
BspPI GGATC 1 cut(s) 422
BspT104I TTCGAA 1 cut(s) 84
BspT107I GGYRCC 1 cut(s) 259
BspTNI GGTCTC 1 cut(s) 236
BsrDI GCAATG 1 cut(s) 678
BsrFI RCCGGY 1 cut(s) 262
BsrI ACTGG 2 cut(s) 490, 546
BssAI RCCGGY 1 cut(s) 262
BssECI CCNNGG 3 cut(s) 237, 382, 400
BssMI GATC 3 cut(s) 427, 502, 599
BssT1I CCWWGG 1 cut(s) 400
Bst4CI ACNGT 1 cut(s) 181
BstAPI GCANNNNNTGC 1 cut(s) 662
BstBI TTCGAA 1 cut(s) 84
BstC8I GCNNGC 1 cut(s) 22
BstDEI CTNAG 4 cut(s) 7, 206, 614, 684
BstDSI CCRYGG 1 cut(s) 382
BstF5I GGATG 2 cut(s) 298, 460
BstKTI GATC 3 cut(s) 430, 505, 602
BstMAI GTCTC 1 cut(s) 236
BstMBI GATC 3 cut(s) 427, 502, 599
BstMWI GCNNNNNNNGC 3 cut(s) 17, 563, 662
BstNSI RCATGY 1 cut(s) 595
BstSCI CCNGG 2 cut(s) 236, 248
BstV1I GCAGC 2 cut(s) 294, 652
BstV2I GAAGAC 2 cut(s) 93, 582
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 382
BtsCI GGATG 2 cut(s) 298, 460
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 2 cut(s) 111, 539
Cac8I GCNNGC 1 cut(s) 22
Cfr10I RCCGGY 1 cut(s) 262
Csp6I GTAC 2 cut(s) 60, 260
CspAI ACCGGT 1 cut(s) 262
CspCI CAANNNNNGTGG 2 cut(s) 364, 399
CviAII CATG 7 cut(s) 94, 278, 303, 450, 500, 592, 629
CviQI GTAC 2 cut(s) 60, 260
DdeI CTNAG 4 cut(s) 7, 206, 614, 684
DpnI GATC 3 cut(s) 429, 504, 601
DpnII GATC 3 cut(s) 427, 502, 599
Eco130I CCWWGG 1 cut(s) 400
Eco31I GGTCTC 1 cut(s) 236
EcoRI GAATTC 1 cut(s) 219
EcoT14I CCWWGG 1 cut(s) 400
ErhI CCWWGG 1 cut(s) 400
FaeI CATG 7 cut(s) 97, 281, 306, 453, 503, 595, 632
FatI CATG 7 cut(s) 93, 277, 302, 449, 499, 591, 628
FbaI TGATCA 1 cut(s) 502
Fnu4HI GCNGC 3 cut(s) 308, 483, 666
FokI GGATG 2 cut(s) 305, 467
Fsp4HI GCNGC 3 cut(s) 308, 483, 666
FspBI CTAG 2 cut(s) 63, 584
GluI GCNGC 3 cut(s) 308, 483, 666
GsaI CCCAGC 1 cut(s) 482
GsuI CTGGAG 1 cut(s) 303
HaeIII GGCC 1 cut(s) 5
HapII CCGG 5 cut(s) 237, 249, 263, 431, 723
Hin1II CATG 7 cut(s) 97, 281, 306, 453, 503, 595, 632
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HindIII AAGCTT 1 cut(s) 518
HinfI GANTC 2 cut(s) 282, 326
HpaI GTTAAC 1 cut(s) 589
HpaII CCGG 5 cut(s) 237, 249, 263, 431, 723
HphI GGTGA 2 cut(s) 409, 639
Hpy166II GTNNAC 1 cut(s) 589
Hpy188I TCNGA 2 cut(s) 574, 639
Hpy188III TCNNGA 1 cut(s) 320
Hpy8I GTNNAC 1 cut(s) 589
HpyAV CCTTC 1 cut(s) 711
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4V TGCA 5 cut(s) 24, 137, 214, 499, 656
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 563, 662
HpyF3I CTNAG 4 cut(s) 7, 206, 614, 684
Hsp92II CATG 7 cut(s) 97, 281, 306, 453, 503, 595, 632
KpnI GGTACC 1 cut(s) 263
Ksp22I TGATCA 1 cut(s) 502
KspAI GTTAAC 1 cut(s) 589
Kzo9I GATC 3 cut(s) 427, 502, 599
LmnI GCTCC 1 cut(s) 115
Lsp1109I GCAGC 2 cut(s) 294, 652
LweI GCATC 1 cut(s) 146
MaeI CTAG 2 cut(s) 63, 584
MaeIII GTNAC 1 cut(s) 181
MalI GATC 3 cut(s) 429, 504, 601
MboI GATC 3 cut(s) 427, 502, 599
MboII GAAGA 4 cut(s) 98, 182, 260, 587
MfeI CAATTG 1 cut(s) 621
MluCI AATT 4 cut(s) 219, 458, 621, 696
MlyI GAGTC 1 cut(s) 276
MmeI TCCRAC 1 cut(s) 511
MnlI CCTC 6 cut(s) 256, 327, 355, 485, 655, 679
MseI TTAA 1 cut(s) 588
MspA1I CMGCKG 2 cut(s) 227, 605
MspI CCGG 5 cut(s) 237, 249, 263, 431, 723
MspR9I CCNGG 2 cut(s) 238, 250
MunI CAATTG 1 cut(s) 621
Mva1269I GAATGC 1 cut(s) 335
MwoI GCNNNNNNNGC 3 cut(s) 17, 563, 662
NciI CCSGG 2 cut(s) 238, 250
NdeII GATC 3 cut(s) 427, 502, 599
NlaIII CATG 7 cut(s) 97, 281, 306, 453, 503, 595, 632
NlaIV GGNNCC 3 cut(s) 111, 261, 388
NspI RCATGY 1 cut(s) 595
NspV TTCGAA 1 cut(s) 84
PciI ACATGT 1 cut(s) 591
PctI GAATGC 1 cut(s) 335
PfeI GAWTC 1 cut(s) 326
PfoI TCCNGGA 1 cut(s) 248
PinAI ACCGGT 1 cut(s) 262
PkrI GCNGC 3 cut(s) 309, 484, 667
PleI GAGTC 1 cut(s) 276
PpsI GAGTC 1 cut(s) 276
PscI ACATGT 1 cut(s) 591
PspFI CCCAGC 1 cut(s) 478
PspN4I GGNNCC 3 cut(s) 111, 261, 388
PvuII CAGCTG 2 cut(s) 227, 605
RsaI GTAC 2 cut(s) 61, 261
RsaNI GTAC 2 cut(s) 60, 260
SaqAI TTAA 1 cut(s) 588
SatI GCNGC 3 cut(s) 308, 483, 666
Sau3AI GATC 3 cut(s) 427, 502, 599
SchI GAGTC 1 cut(s) 276
ScrFI CCNGG 2 cut(s) 238, 250
SfaNI GCATC 1 cut(s) 146
SfuI TTCGAA 1 cut(s) 84
Sse9I AATT 4 cut(s) 219, 458, 621, 696
SsiI CCGC 1 cut(s) 483
SspMI CTAG 2 cut(s) 63, 584
StyD4I CCNGG 2 cut(s) 236, 248
StyI CCWWGG 1 cut(s) 400
TaaI ACNGT 1 cut(s) 181
TaqI TCGA 1 cut(s) 84
TasI AATT 4 cut(s) 219, 458, 621, 696
TauI GCSGC 1 cut(s) 485
TfiI GAWTC 1 cut(s) 326
Tru1I TTAA 1 cut(s) 588
Tru9I TTAA 1 cut(s) 588
TscAI CASTG 2 cut(s) 111, 546
TseI GCWGC 2 cut(s) 307, 665
TspDTI ATGAA 3 cut(s) 328, 471, 667
TspRI CASTG 2 cut(s) 111, 546
XapI RAATTY 1 cut(s) 219
XceI RCATGY 1 cut(s) 595
XspI CTAG 2 cut(s) 63, 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.