Rroxscaffold_2G00091890
ERF Family

Belongs to the AB hydrolase superfamily. Lipase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000628
Physical Location & Seq
Forward (+)
13410286 .. 13412244
1959 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00091890.1

Sequence Viewer

Length: 1230 bp
ATGAAAATGGCAAACATTCTTACCTCTATTACTGCAGTTGTTCTGTTCTGGGGATTGGCAGTTGGTACTAGAACAAAGCTATTCTCTTTCGAAGACAGCGTGGTTTCGGCAGTGGCTCCATCTGGTAGTGATGGTATCTGTGCATCAATGGTGCTATCACAAGGCTACCCTTGTGAAGAACATACAGTAACAACACAAGATGGCTACATACTCAGTATGCAGAGAATTCCAGCTGGTAAGTCCGGGGAGACCTCCGGGAACAAGGTACCGGTTCTTCTACAACATGGACTCCTTATGGATGGGATAACATGGCTGCTACTACCTCCAGACGAATCTTTGGCATTCAACTTGGCAGATAATGGGTATGAGGTGTGGATTGCCAATACCCGTGGAACCAAATATAGCCTTGGACATACATCACTCACCCCCGATGATCCGGCTTATTGGGAGTGGTCATGGGATGAATTGGTCGCTTATGACCTCCCAGCCGCATACCAGTATGTGCATGATCAAGCAGGACAGAAGCTTCACTATGTTGGACATTCACTGGGAACTTTGATTGCTCTTGGTGCTTTTTCAGAAGACCAGCTAGTTAACATGTTGAGATCAGCTGTCTTACTTAGCCCAATTGCTCATGTTGGTCAGATAACCTCACCCCTTGCAAAAACTGCTGCTGAAAACTTCATTGCTGAGGATTTATACAAATCAGGTTTTAGAGAATTTGATCCAAAAGGACCAATGATAGTCCAAGTTCTTAAGGAAATCTGCTCCAAACCAGGCGCTGACTGCACAAACTTGATGACCTCTTTCACAGGCCAGAATTGCTGCTTAAATTCATCCATTACCGACATTTTTCTAGACCATGAGCCTCAGGCAACTTCAATAAAAAACATGATCCACCTCGCTCAGATGATAAGAGATGGGAAAGTTGCGATGTTCGACTACAATGACAAGGAGGAGAACCAACAACACTACGGTCAGGACACCCCTCCAGAGTACAAAGTGGCGAGCATACCGCATGACATTCCTCTCTTCCTCGGTTATGGAGGGGCAGATGCGCTTTCTGATGTCCAAGATGTGAAGCTATTGCTCAACAGCCTCAAAGATCATGATGGAGATAAGCTTGTGATTCAGTACATAGATGATTATGCTCATGCAGATTTTGTCATGGGTGAAAATGCTAAACAAGATGTGTATGATCCTCTCATGGCTTTCTTCAAACTTCAGTGA

Protein Analysis

409

Amino Acids

44.99

Weight (kDa)

4.7

Isoelectric Point (pI)

24.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydro_lipase PF04083 49 - 106 1.2e-21 Partial alpha/beta-hydrolase lipase region
Abhydrolase_1 PF00561 90 - 216 4.5e-10 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000628)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14180 AT5G14180 AT5G14180 AT5G14180 AT5G14180
fragaria_vesca FvH4_6g41960 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g53200
malus_domestica MD09G1013100.v1.1 MD09G1115500.v1.1 MD09G1115600.v1.1 MD09G1115800.v1.1 MD17G1013800.v1.1 MD17G1107400.v1.1
prunus_persica Prupe.3G208900_v2.0.a1 Prupe.3G208900_v2.0.a1 Prupe.3G304100_v2.0.a1
pyrus_communis pycom09g04000 pycom09g04020 pycom09g04040 pycom111g01090 pycom111g01110 pycom17g01090 pycom17g10010
rosa_chinensis RchiOBHm_Chr2g0157861 RchiOBHm_Chr2g0157881 RchiOBHm_Chr2g0175241 RchiOBHm_Chr2g0175251
rosa_laevigata RLG00000021032 RLG00000021033 RLG00000022314 RLG00000022315
rosa_multiflora Rmu_co8368271.1_g000001 Rmu_co8519319.1_g000001 Rmu_co8519319.1_g000002 Rmu_sc0002441.1_g000007 Rmu_sc0002441.1_g000008 Rmu_sc0010083.1_g000002 Rmu_sc0015795.1_g000001 Rmu_sc0015795.1_g000002
rosa_roxburghii Rroxscaffold_1G00036740 Rroxscaffold_2G00077450 Rroxscaffold_2G00077460 Rroxscaffold_2G00091880 Rroxscaffold_2G00091890 Rroxscaffold_2G00127210
rosa_rugosa Rorug02G0464200 Rorug02G0464200 Rorug02G0582000 Rorug02G0582100 Rorug02G0582100 Rorug02G0582100 Rorug02G0582200 Rorug02G0582200 Rorug02G0582200
rosa_samantha Rh2AG529400 Rh2AG529500 Rh2AG662400 Rh2AG662500 Rh2BG544000 Rh2BG544100 Rh2BG673600 Rh2BG673700 Rh2CG513700 Rh2CG513800 Rh2CG636800 Rh2DG552400
rosa_wichuraiana Rw2G043800 Rw2G043810 Rw2G054330 Rw2G054340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 265
AccB1I GGYRCC 1 cut(s) 265
AciI CCGC 2 cut(s) 489, 1016
AclWI GGATC 4 cut(s) 428, 719, 889, 1193
AcsI RAATTY 3 cut(s) 225, 719, 832
AcuI CTGAAG 1 cut(s) 1208
AfaI GTAC 4 cut(s) 67, 267, 998, 1136
AflII CTTAAG 1 cut(s) 755
AflIII ACRYGT 1 cut(s) 597
AgeI ACCGGT 1 cut(s) 268
AgsI TTSAA 3 cut(s) 346, 882, 1219
AjnI CCWGG 1 cut(s) 775
AluBI AGCT 7 cut(s) 79, 233, 526, 589, 611, 1084, 1123
AluI AGCT 7 cut(s) 79, 233, 526, 589, 611, 1084, 1123
Alw26I GTCTC 1 cut(s) 242
AlwI GGATC 4 cut(s) 428, 719, 889, 1193
AlwNI CAGNNNCTG 1 cut(s) 782
AoxI GGCC 1 cut(s) 814
ApeKI GCWGC 3 cut(s) 313, 671, 825
ApoI RAATTY 3 cut(s) 225, 719, 832
AsiGI ACCGGT 1 cut(s) 268
Asp718I GGTACC 1 cut(s) 265
AspLEI GCGC 2 cut(s) 782, 1060
AspS9I GGNCC 1 cut(s) 734
AsuC2I CCSGG 2 cut(s) 244, 256
AsuHPI GGTGA 3 cut(s) 415, 645, 1184
AsuII TTCGAA 1 cut(s) 90
AvaII GGWCC 1 cut(s) 734
AxyI CCTNAGG 1 cut(s) 870
BanI GGYRCC 1 cut(s) 265
BarI GAAGNNNNNNTAC 2 cut(s) 258, 290
BbsI GAAGAC 2 cut(s) 99, 588
BbvCI CCTCAGC 1 cut(s) 690
BbvI GCAGC 3 cut(s) 300, 658, 812
BccI CCATC 6 cut(s) 125, 127, 194, 293, 914, 1106
BciT130I CCWGG 1 cut(s) 777
BclI TGATCA 1 cut(s) 508
BcnI CCSGG 2 cut(s) 244, 256
BcoDI GTCTC 1 cut(s) 242
BfaI CTAG 3 cut(s) 69, 590, 857
BfmI CTRYAG 1 cut(s) 33
BfoI RGCGCY 1 cut(s) 783
BfrI CTTAAG 1 cut(s) 755
BisI GCNGC 4 cut(s) 314, 489, 672, 826
BlsI GCNGC 4 cut(s) 315, 490, 673, 827
Bme1390I CCNGG 3 cut(s) 244, 256, 777
Bme18I GGWCC 1 cut(s) 734
BmgT120I GGNCC 1 cut(s) 734
BmiI GGNNCC 3 cut(s) 117, 267, 394
BmrFI CCNGG 3 cut(s) 244, 256, 777
BmrI ACTGGG 1 cut(s) 557
BmsI GCATC 2 cut(s) 152, 1045
BmuI ACTGGG 1 cut(s) 557
BpiI GAAGAC 2 cut(s) 99, 588
BpmI CTGGAG 2 cut(s) 309, 975
Bpu10I CCTNAGC 1 cut(s) 690
Bpu14I TTCGAA 1 cut(s) 90
BpuMI CCSGG 2 cut(s) 244, 256
BsaI GGTCTC 1 cut(s) 242
BsaJI CCNNGG 4 cut(s) 243, 388, 406, 1036
BsaWI WCCGGW 1 cut(s) 268
BsaXI ACNNNNNCTCC 2 cut(s) 273, 303
Bse118I RCCGGY 1 cut(s) 268
Bse1I ACTGG 2 cut(s) 496, 552
Bse21I CCTNAGG 1 cut(s) 870
Bse3DI GCAATG 1 cut(s) 684
BseBI CCWGG 1 cut(s) 777
BseDI CCNNGG 4 cut(s) 243, 388, 406, 1036
BseGI GGATG 3 cut(s) 304, 466, 836
BseMI GCAATG 1 cut(s) 684
BseMII CTCAG 4 cut(s) 226, 681, 884, 920
BseNI ACTGG 2 cut(s) 496, 552
BseRI GAGGAG 1 cut(s) 971
BseXI GCAGC 3 cut(s) 300, 658, 812
BseYI CCCAGC 1 cut(s) 484
BsgI GTGCAG 1 cut(s) 772
BshFI GGCC 1 cut(s) 816
BshNI GGYRCC 1 cut(s) 265
BshTI ACCGGT 1 cut(s) 268
BsiSI CCGG 4 cut(s) 243, 255, 269, 437
BsmAI GTCTC 1 cut(s) 242
BsmI GAATGC 1 cut(s) 341
BsnI GGCC 1 cut(s) 816
Bso31I GGTCTC 1 cut(s) 242
Bsp119I TTCGAA 1 cut(s) 90
Bsp143I GATC 7 cut(s) 433, 508, 605, 724, 894, 1105, 1198
BspACI CCGC 2 cut(s) 489, 1016
BspANI GGCC 1 cut(s) 816
BspCNI CTCAG 4 cut(s) 225, 682, 883, 919
BspHI TCATGA 1 cut(s) 1108
BspLI GGNNCC 3 cut(s) 117, 267, 394
BspMAI CTGCAG 1 cut(s) 37
BspPI GGATC 4 cut(s) 428, 719, 889, 1193
BspT104I TTCGAA 1 cut(s) 90
BspT107I GGYRCC 1 cut(s) 265
BspTI CTTAAG 1 cut(s) 755
BspTNI GGTCTC 1 cut(s) 242
BsrDI GCAATG 1 cut(s) 684
BsrFI RCCGGY 1 cut(s) 268
BsrI ACTGG 2 cut(s) 496, 552
BssAI RCCGGY 1 cut(s) 268
BssECI CCNNGG 4 cut(s) 243, 388, 406, 1036
BssMI GATC 7 cut(s) 433, 508, 605, 724, 894, 1105, 1198
BssT1I CCWWGG 1 cut(s) 406
Bst2UI CCWGG 1 cut(s) 777
Bst4CI ACNGT 2 cut(s) 187, 977
Bst6I CTCTTC 1 cut(s) 1037
BstAFI CTTAAG 1 cut(s) 755
BstAPI GCANNNNNTGC 1 cut(s) 668
BstBI TTCGAA 1 cut(s) 90
BstC8I GCNNGC 1 cut(s) 1009
BstDEI CTNAG 5 cut(s) 212, 620, 690, 870, 906
BstDSI CCRYGG 1 cut(s) 388
BstF5I GGATG 3 cut(s) 304, 466, 836
BstH2I RGCGCY 1 cut(s) 783
BstHHI GCGC 2 cut(s) 782, 1060
BstKTI GATC 7 cut(s) 436, 511, 608, 727, 897, 1108, 1201
BstMAI GTCTC 1 cut(s) 242
BstMBI GATC 7 cut(s) 433, 508, 605, 724, 894, 1105, 1198
BstMWI GCNNNNNNNGC 4 cut(s) 569, 668, 786, 822
BstNI CCWGG 1 cut(s) 777
BstNSI RCATGY 1 cut(s) 601
BstSCI CCNGG 3 cut(s) 242, 254, 775
BstSFI CTRYAG 1 cut(s) 33
BstV1I GCAGC 3 cut(s) 300, 658, 812
BstV2I GAAGAC 2 cut(s) 99, 588
Bsu36I CCTNAGG 1 cut(s) 870
BsuRI GGCC 1 cut(s) 816
BtgI CCRYGG 1 cut(s) 388
BtgZI GCGATG 1 cut(s) 947
BtsCI GGATG 3 cut(s) 304, 466, 836
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 2 cut(s) 117, 545
Cac8I GCNNGC 1 cut(s) 1009
CaiI CAGNNNCTG 1 cut(s) 782
CciI TCATGA 1 cut(s) 1108
CfoI GCGC 2 cut(s) 782, 1060
Cfr10I RCCGGY 1 cut(s) 268
Cfr13I GGNCC 1 cut(s) 734
Csp6I GTAC 4 cut(s) 66, 266, 997, 1135
CspAI ACCGGT 1 cut(s) 268
CspCI CAANNNNNGTGG 2 cut(s) 370, 405
CviQI GTAC 4 cut(s) 66, 266, 997, 1135
DdeI CTNAG 5 cut(s) 212, 620, 690, 870, 906
DpnI GATC 7 cut(s) 435, 510, 607, 726, 896, 1107, 1200
DpnII GATC 7 cut(s) 433, 508, 605, 724, 894, 1105, 1198
Eam1104I CTCTTC 1 cut(s) 1037
EarI CTCTTC 1 cut(s) 1037
Eco130I CCWWGG 1 cut(s) 406
Eco31I GGTCTC 1 cut(s) 242
Eco47I GGWCC 1 cut(s) 734
Eco57I CTGAAG 1 cut(s) 1208
Eco81I CCTNAGG 1 cut(s) 870
EcoRI GAATTC 1 cut(s) 225
EcoRII CCWGG 1 cut(s) 775
EcoT14I CCWWGG 1 cut(s) 406
ErhI CCWWGG 1 cut(s) 406
FbaI TGATCA 1 cut(s) 508
Fnu4HI GCNGC 4 cut(s) 314, 489, 672, 826
FokI GGATG 3 cut(s) 311, 473, 823
Fsp4HI GCNGC 4 cut(s) 314, 489, 672, 826
FspBI CTAG 3 cut(s) 69, 590, 857
GlaI GCGC 2 cut(s) 781, 1059
GluI GCNGC 4 cut(s) 314, 489, 672, 826
GsaI CCCAGC 1 cut(s) 488
GsuI CTGGAG 2 cut(s) 309, 975
HaeII RGCGCY 1 cut(s) 783
HaeIII GGCC 1 cut(s) 816
HapII CCGG 4 cut(s) 243, 255, 269, 437
HhaI GCGC 2 cut(s) 782, 1060
Hin6I GCGC 2 cut(s) 780, 1058
HinP1I GCGC 2 cut(s) 780, 1058
HincII GTYRAC 1 cut(s) 595
HindII GTYRAC 1 cut(s) 595
HindIII AAGCTT 2 cut(s) 524, 1121
HinfI GANTC 3 cut(s) 288, 332, 1129
HpaI GTTAAC 1 cut(s) 595
HpaII CCGG 4 cut(s) 243, 255, 269, 437
HphI GGTGA 3 cut(s) 415, 645, 1184
Hpy166II GTNNAC 1 cut(s) 595
Hpy188I TCNGA 4 cut(s) 580, 645, 909, 1066
Hpy188III TCNNGA 5 cut(s) 326, 857, 980, 992, 1109
Hpy8I GTNNAC 1 cut(s) 595
HpyCH4III ACNGT 2 cut(s) 187, 977
HpyCH4V TGCA 7 cut(s) 35, 143, 220, 505, 662, 789, 1157
HpyF10VI GCNNNNNNNGC 4 cut(s) 569, 668, 786, 822
HpyF3I CTNAG 5 cut(s) 212, 620, 690, 870, 906
HspAI GCGC 2 cut(s) 780, 1058
KpnI GGTACC 1 cut(s) 269
Ksp22I TGATCA 1 cut(s) 508
KspAI GTTAAC 1 cut(s) 595
Kzo9I GATC 7 cut(s) 433, 508, 605, 724, 894, 1105, 1198
LmnI GCTCC 2 cut(s) 121, 773
Lsp1109I GCAGC 3 cut(s) 300, 658, 812
LweI GCATC 2 cut(s) 152, 1045
MaeI CTAG 3 cut(s) 69, 590, 857
MaeIII GTNAC 1 cut(s) 187
MalI GATC 7 cut(s) 435, 510, 607, 726, 896, 1107, 1200
MboI GATC 7 cut(s) 433, 508, 605, 724, 894, 1105, 1198
MboII GAAGA 6 cut(s) 104, 188, 266, 593, 1024, 1207
MfeI CAATTG 1 cut(s) 627
MluCI AATT 6 cut(s) 225, 464, 627, 719, 820, 832
MlyI GAGTC 1 cut(s) 282
MmeI TCCRAC 1 cut(s) 517
MseI TTAA 3 cut(s) 594, 756, 830
MspA1I CMGCKG 2 cut(s) 233, 611
MspCI CTTAAG 1 cut(s) 755
MspI CCGG 4 cut(s) 243, 255, 269, 437
MspR9I CCNGG 3 cut(s) 244, 256, 777
MunI CAATTG 1 cut(s) 627
Mva1269I GAATGC 1 cut(s) 341
MvaI CCWGG 1 cut(s) 777
MwoI GCNNNNNNNGC 4 cut(s) 569, 668, 786, 822
NciI CCSGG 2 cut(s) 244, 256
NdeII GATC 7 cut(s) 433, 508, 605, 724, 894, 1105, 1198
NlaIV GGNNCC 3 cut(s) 117, 267, 394
NspI RCATGY 1 cut(s) 601
NspV TTCGAA 1 cut(s) 90
PagI TCATGA 1 cut(s) 1108
PciI ACATGT 1 cut(s) 597
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PctI GAATGC 1 cut(s) 341
PfeI GAWTC 2 cut(s) 332, 1129
PfoI TCCNGGA 1 cut(s) 254
PinAI ACCGGT 1 cut(s) 268
PkrI GCNGC 4 cut(s) 315, 490, 673, 827
PleI GAGTC 1 cut(s) 282
PpsI GAGTC 1 cut(s) 282
PscI ACATGT 1 cut(s) 597
Psp6I CCWGG 1 cut(s) 775
PspFI CCCAGC 1 cut(s) 484
PspGI CCWGG 1 cut(s) 775
PspN4I GGNNCC 3 cut(s) 117, 267, 394
PspPI GGNCC 1 cut(s) 734
PstI CTGCAG 1 cut(s) 37
PstNI CAGNNNCTG 1 cut(s) 782
PvuII CAGCTG 2 cut(s) 233, 611
RsaI GTAC 4 cut(s) 67, 267, 998, 1136
RsaNI GTAC 4 cut(s) 66, 266, 997, 1135
SaqAI TTAA 3 cut(s) 594, 756, 830
SatI GCNGC 4 cut(s) 314, 489, 672, 826
Sau3AI GATC 7 cut(s) 433, 508, 605, 724, 894, 1105, 1198
Sau96I GGNCC 1 cut(s) 734
SchI GAGTC 1 cut(s) 282
ScrFI CCNGG 3 cut(s) 244, 256, 777
SfaNI GCATC 2 cut(s) 152, 1045
SfcI CTRYAG 1 cut(s) 33
SfuI TTCGAA 1 cut(s) 90
SinI GGWCC 1 cut(s) 734
SmlI CTYRAG 1 cut(s) 755
SmoI CTYRAG 1 cut(s) 755
Sse9I AATT 6 cut(s) 225, 464, 627, 719, 820, 832
SsiI CCGC 2 cut(s) 489, 1016
SspMI CTAG 3 cut(s) 69, 590, 857
StyD4I CCNGG 3 cut(s) 242, 254, 775
StyI CCWWGG 1 cut(s) 406
TaaI ACNGT 2 cut(s) 187, 977
TaqI TCGA 2 cut(s) 90, 939
TasI AATT 6 cut(s) 225, 464, 627, 719, 820, 832
TatI WGTACW 2 cut(s) 996, 1134
TauI GCSGC 1 cut(s) 491
TfiI GAWTC 2 cut(s) 332, 1129
Tru1I TTAA 3 cut(s) 594, 756, 830
Tru9I TTAA 3 cut(s) 594, 756, 830
TscAI CASTG 2 cut(s) 117, 552
TseI GCWGC 3 cut(s) 313, 671, 825
TspDTI ATGAA 4 cut(s) 17, 477, 673, 825
TspRI CASTG 2 cut(s) 117, 552
Vha464I CTTAAG 1 cut(s) 755
VpaK11BI GGWCC 1 cut(s) 734
XapI RAATTY 3 cut(s) 225, 719, 832
XbaI TCTAGA 1 cut(s) 856
XceI RCATGY 1 cut(s) 601
XspI CTAG 3 cut(s) 69, 590, 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.