Prupe.3G304100_v2.0.a1
ERF Family

Belongs to the AB hydrolase superfamily. Lipase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000628
Physical Location & Seq
Forward (+)
26712703 .. 26715349
2647 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G304100.1

Sequence Viewer

Length: 1227 bp
ATGGCTGCAACCTTGGCTAGCTTTTGCCTCCTAGTCTTGGTGGCAGCCCTAGCACCTCATCGAGCTTACGCCTCCATTCGTAGCTCACTTGGCCACAAACGTGCTGCTGCTGCGGCTGCCCCAACCGTCGGTATATGTGCGTCTTCGGTGCTTGTATATGGCTACCAATGCCAGGAGCTTCAGGTGACAACCCAAGATGGTTACATACTGAGTTTGCAAAGAATCCCAGAAGGTCGTCATAGTAATGGGACTAAGAAGCCGCCGGTCTTAATTCAACATGGTGTCCTTGTGGATGGAGTCACCTGGCTCTTAAATTCTCCGGATAGAAATCTACCGTTGATTTTGGCTGATAATGGGTTTGATGTGTGGATTGCTAACACCAGAGGGACCAGGTTTAGCCGACGACATACTTCCCTGGACCCCTCCGATCCGAAATTTTGGAATTGGTCTTGGGATGAACTGGTGGCCTTTGACCTACCAGCTGTTTTTGATTTCGTGTATAGCAAAACAGGACAGAAGGTTAATTACGTGGGCCATTCACTGGGTACTTTGTTTGCATTGGCATCGTTATCAGATGGGAAACTGGTGGAGCAGATGAAGTCGGCAGCCTTGTTGAGCCCCATTGCTTATTTGAGTCACATGAACACTGCACTTGGCGTGGCTGCTGCAAAGGCCTTTGTTGGTGAGATCACTACGCTATTCGGTCTTGCCGAGTTCAATCCAAAAGGGGAGCCTGTGGCCCAATTTCTCAATGCTCTGTGTGTTTATCCAGGGGTTGACTGCTATGACTTACTAGAAGCAGTTACTGGAAAAAACTGCTGCCTCAATTCTTCCACTGTTGATCTCTTCCTGAAGAATGAGCCTCAGTCAACATCCACCAGGAACATGGTGCACTTGGCTCAAACTGTGCGAGATGGGGTGTTGGCAAAATACAACTACGGGAGACTAGACTACAATTTGATGCATTATGGGAAATTTAGCCCTCCAATTTATAACCTATCCAACATTCCCCATAACCTTCCTCTGTTCCTCAGCTATGGCGGCCGAGACTCGCTCTCTGATATTCGGGACGTGGAGCTTTTACTTGATATTCTTAAGCTTCATGATGTGGGCAAGCTGACTGTTCAGTACATAAAAGACTATGCACATGCAGACTTCATCATGGGGTTGAATGCCAAGGACATTGTGTACAATCAAGTTACTGCCTTTTTCCAGCAGCAACAATAA

Protein Analysis

409

Amino Acids

44.87

Weight (kDa)

6.85

Isoelectric Point (pI)

29.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000628)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14180 AT5G14180 AT5G14180 AT5G14180 AT5G14180
fragaria_vesca FvH4_6g41960 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g41980 FvH4_6g53200
malus_domestica MD09G1013100.v1.1 MD09G1115500.v1.1 MD09G1115600.v1.1 MD09G1115800.v1.1 MD17G1013800.v1.1 MD17G1107400.v1.1
prunus_persica Prupe.3G208900_v2.0.a1 Prupe.3G208900_v2.0.a1 Prupe.3G304100_v2.0.a1
pyrus_communis pycom09g04000 pycom09g04020 pycom09g04040 pycom111g01090 pycom111g01110 pycom17g01090 pycom17g10010
rosa_chinensis RchiOBHm_Chr2g0157861 RchiOBHm_Chr2g0157881 RchiOBHm_Chr2g0175241 RchiOBHm_Chr2g0175251
rosa_laevigata RLG00000021032 RLG00000021033 RLG00000022314 RLG00000022315
rosa_multiflora Rmu_co8368271.1_g000001 Rmu_co8519319.1_g000001 Rmu_co8519319.1_g000002 Rmu_sc0002441.1_g000007 Rmu_sc0002441.1_g000008 Rmu_sc0010083.1_g000002 Rmu_sc0015795.1_g000001 Rmu_sc0015795.1_g000002
rosa_roxburghii Rroxscaffold_1G00036740 Rroxscaffold_2G00077450 Rroxscaffold_2G00077460 Rroxscaffold_2G00091880 Rroxscaffold_2G00091890 Rroxscaffold_2G00127210
rosa_rugosa Rorug02G0464200 Rorug02G0464200 Rorug02G0582000 Rorug02G0582100 Rorug02G0582100 Rorug02G0582100 Rorug02G0582200 Rorug02G0582200 Rorug02G0582200
rosa_samantha Rh2AG529400 Rh2AG529500 Rh2AG662400 Rh2AG662500 Rh2BG544000 Rh2BG544100 Rh2BG673600 Rh2BG673700 Rh2CG513700 Rh2CG513800 Rh2CG636800 Rh2DG552400
rosa_wichuraiana Rw2G043800 Rw2G043810 Rw2G054330 Rw2G054340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 993
AccB7I CCANNNNNTGG 1 cut(s) 541
AccIII TCCGGA 1 cut(s) 319
AciI CCGC 3 cut(s) 113, 260, 1041
AclWI GGATC 1 cut(s) 422
AcoI YGGCCR 2 cut(s) 91, 1042
AcsI RAATTY 3 cut(s) 313, 434, 974
AcuI CTGAAG 2 cut(s) 164, 872
AfaI GTAC 3 cut(s) 547, 1130, 1190
AfiI CCNNNNNNNGG 4 cut(s) 37, 128, 172, 541
AflII CTTAAG 1 cut(s) 1094
AgsI TTSAA 3 cut(s) 275, 718, 1171
AjiI CACGTC 1 cut(s) 1072
AjnI CCWGG 6 cut(s) 171, 302, 389, 414, 769, 878
AleI CACNNNNGTG 1 cut(s) 99
AluBI AGCT 9 cut(s) 21, 65, 84, 178, 482, 1035, 1078, 1099, 1117
AluI AGCT 9 cut(s) 21, 65, 84, 178, 482, 1035, 1078, 1099, 1117
Alw21I GWGCWC 1 cut(s) 894
Alw26I GTCTC 2 cut(s) 937, 1041
Alw44I GTGCAC 1 cut(s) 890
AlwI GGATC 1 cut(s) 422
AlwNI CAGNNNCTG 1 cut(s) 806
Aor13HI TCCGGA 1 cut(s) 319
AoxI GGCC 6 cut(s) 91, 465, 532, 672, 738, 1042
ApaLI GTGCAC 1 cut(s) 890
ApoI RAATTY 3 cut(s) 313, 434, 974
AspS9I GGNCC 4 cut(s) 387, 418, 532, 739
AsuHPI GGTGA 3 cut(s) 196, 292, 695
AsuNHI GCTAGC 1 cut(s) 17
AvaII GGWCC 2 cut(s) 387, 418
BaeGI GKGCMC 1 cut(s) 894
BalI TGGCCA 1 cut(s) 93
BanII GRGCYC 1 cut(s) 620
BarI GAAGNNNNNNTAC 2 cut(s) 509, 541
BbsI GAAGAC 1 cut(s) 135
Bbv12I GWGCWC 1 cut(s) 894
BbvCI CCTCAGC 1 cut(s) 1031
BbvI GCAGC 9 cut(s) 56, 91, 94, 97, 103, 617, 649, 652, 806
BccI CCATC 4 cut(s) 191, 287, 569, 908
BcgI CGANNNNNNTGC 2 cut(s) 546, 580
BciT130I CCWGG 6 cut(s) 173, 304, 391, 416, 771, 880
BcoDI GTCTC 2 cut(s) 937, 1041
BfaI CTAG 5 cut(s) 18, 32, 50, 794, 947
BfrI CTTAAG 1 cut(s) 1094
Bme1390I CCNGG 6 cut(s) 173, 304, 391, 416, 771, 880
Bme18I GGWCC 2 cut(s) 387, 418
BmgBI CACGTC 1 cut(s) 1072
BmgT120I GGNCC 4 cut(s) 387, 418, 532, 739
BmiI GGNNCC 3 cut(s) 388, 420, 732
BmrFI CCNGG 6 cut(s) 173, 304, 391, 416, 771, 880
BmrI ACTGGG 1 cut(s) 551
BmsI GCATC 2 cut(s) 572, 951
BmtI GCTAGC 1 cut(s) 21
BmuI ACTGGG 1 cut(s) 551
BpiI GAAGAC 1 cut(s) 135
BplI GAGNNNNNCTC 2 cut(s) 1038, 1070
Bpu10I CCTNAGC 1 cut(s) 1031
BsaAI YACGTR 1 cut(s) 529
BsaBI GATNNNNATC 1 cut(s) 327
BsaJI CCNNGG 4 cut(s) 12, 414, 770, 1176
BsaWI WCCGGW 1 cut(s) 319
Bsc4I CCNNNNNNNGG 4 cut(s) 37, 128, 172, 541
Bse118I RCCGGY 1 cut(s) 262
Bse1I ACTGG 4 cut(s) 465, 546, 588, 811
Bse3DI GCAATG 1 cut(s) 621
Bse8I GATNNNNATC 1 cut(s) 327
BseAI TCCGGA 1 cut(s) 319
BseBI CCWGG 6 cut(s) 173, 304, 391, 416, 771, 880
BseDI CCNNGG 4 cut(s) 12, 414, 770, 1176
BseGI GGATG 3 cut(s) 298, 460, 872
BseJI GATNNNNATC 1 cut(s) 327
BseLI CCNNNNNNNGG 4 cut(s) 37, 128, 172, 541
BseMI GCAATG 1 cut(s) 621
BseMII CTCAG 3 cut(s) 200, 878, 1045
BseNI ACTGG 4 cut(s) 465, 546, 588, 811
BseSI GKGCMC 1 cut(s) 894
BseX3I CGGCCG 1 cut(s) 1042
BseXI GCAGC 9 cut(s) 56, 91, 94, 97, 103, 617, 649, 652, 806
BsgI GTGCAG 1 cut(s) 633
Bsh1285I CGRYCG 1 cut(s) 1045
BshFI GGCC 6 cut(s) 93, 467, 534, 674, 740, 1044
BsiEI CGRYCG 1 cut(s) 1045
BsiHKAI GWGCWC 1 cut(s) 894
BsiSI CCGG 2 cut(s) 263, 320
BslFI GGGAC 3 cut(s) 262, 400, 1082
BslI CCNNNNNNNGG 4 cut(s) 37, 128, 172, 541
BsmAI GTCTC 2 cut(s) 937, 1041
BsmFI GGGAC 3 cut(s) 262, 400, 1082
BsmI GAATGC 1 cut(s) 1177
BsnI GGCC 6 cut(s) 93, 467, 534, 674, 740, 1044
Bsp1286I GDGCHC 2 cut(s) 620, 894
Bsp13I TCCGGA 1 cut(s) 319
Bsp1407I TGTACA 1 cut(s) 1188
Bsp143I GATC 3 cut(s) 427, 687, 841
BspACI CCGC 3 cut(s) 113, 260, 1041
BspANI GGCC 6 cut(s) 93, 467, 534, 674, 740, 1044
BspCNI CTCAG 3 cut(s) 201, 877, 1044
BspEI TCCGGA 1 cut(s) 319
BspHI TCATGA 1 cut(s) 1102
BspLI GGNNCC 3 cut(s) 388, 420, 732
BspOI GCTAGC 1 cut(s) 21
BspPI GGATC 1 cut(s) 422
BspTI CTTAAG 1 cut(s) 1094
BsrDI GCAATG 1 cut(s) 621
BsrFI RCCGGY 1 cut(s) 262
BsrGI TGTACA 1 cut(s) 1188
BsrI ACTGG 4 cut(s) 465, 546, 588, 811
BssAI RCCGGY 1 cut(s) 262
BssECI CCNNGG 4 cut(s) 12, 414, 770, 1176
BssMI GATC 3 cut(s) 427, 687, 841
BssT1I CCWWGG 2 cut(s) 12, 1176
Bst2UI CCWGG 6 cut(s) 173, 304, 391, 416, 771, 880
Bst4CI ACNGT 5 cut(s) 127, 336, 838, 907, 1123
Bst6I CTCTTC 1 cut(s) 851
BstAFI CTTAAG 1 cut(s) 1094
BstAUI TGTACA 1 cut(s) 1188
BstBAI YACGTR 1 cut(s) 529
BstC8I GCNNGC 2 cut(s) 19, 1115
BstDEI CTNAG 4 cut(s) 209, 252, 864, 1031
BstF5I GGATG 3 cut(s) 298, 460, 872
BstKTI GATC 3 cut(s) 430, 690, 844
BstMAI GTCTC 2 cut(s) 937, 1041
BstMBI GATC 3 cut(s) 427, 687, 841
BstMCI CGRYCG 1 cut(s) 1045
BstMWI GCNNNNNNNGC 9 cut(s) 14, 50, 90, 110, 113, 116, 168, 671, 1041
BstNI CCWGG 6 cut(s) 173, 304, 391, 416, 771, 880
BstNSI RCATGY 1 cut(s) 1151
BstSCI CCNGG 6 cut(s) 171, 302, 389, 414, 769, 878
BstSLI GKGCMC 1 cut(s) 894
BstV1I GCAGC 9 cut(s) 56, 91, 94, 97, 103, 617, 649, 652, 806
BstV2I GAAGAC 1 cut(s) 135
BstXI CCANNNNNNTGG 1 cut(s) 886
BstZI CGGCCG 1 cut(s) 1042
BsuRI GGCC 6 cut(s) 93, 467, 534, 674, 740, 1044
BtrI CACGTC 1 cut(s) 1072
BtsCI GGATG 3 cut(s) 298, 460, 872
BtsI GCAGTG 1 cut(s) 645
BtsIMutI CAGTG 3 cut(s) 539, 645, 834
Cac8I GCNNGC 2 cut(s) 19, 1115
CaiI CAGNNNCTG 1 cut(s) 806
CciI TCATGA 1 cut(s) 1102
Cfr10I RCCGGY 1 cut(s) 262
Cfr13I GGNCC 4 cut(s) 387, 418, 532, 739
CseI GACGC 1 cut(s) 129
CsiI ACCWGGT 1 cut(s) 389
Csp6I GTAC 3 cut(s) 546, 1129, 1189
CviAII CATG 6 cut(s) 278, 640, 886, 1103, 1148, 1162
CviQI GTAC 3 cut(s) 546, 1129, 1189
DdeI CTNAG 4 cut(s) 209, 252, 864, 1031
DpnI GATC 3 cut(s) 429, 689, 843
DpnII GATC 3 cut(s) 427, 687, 841
EaeI YGGCCR 2 cut(s) 91, 1042
EagI CGGCCG 1 cut(s) 1042
Eam1104I CTCTTC 1 cut(s) 851
EarI CTCTTC 1 cut(s) 851
EclXI CGGCCG 1 cut(s) 1042
Eco130I CCWWGG 2 cut(s) 12, 1176
Eco147I AGGCCT 1 cut(s) 674
Eco24I GRGCYC 1 cut(s) 620
Eco47I GGWCC 2 cut(s) 387, 418
Eco52I CGGCCG 1 cut(s) 1042
Eco57I CTGAAG 2 cut(s) 164, 872
EcoRII CCWGG 6 cut(s) 171, 302, 389, 414, 769, 878
EcoT14I CCWWGG 2 cut(s) 12, 1176
EcoT22I ATGCAT 1 cut(s) 966
EcoT38I GRGCYC 1 cut(s) 620
ErhI CCWWGG 2 cut(s) 12, 1176
FaeI CATG 6 cut(s) 281, 643, 889, 1106, 1151, 1165
FaqI GGGAC 3 cut(s) 262, 400, 1082
FatI CATG 6 cut(s) 277, 639, 885, 1102, 1147, 1161
FokI GGATG 3 cut(s) 305, 467, 859
FriOI GRGCYC 1 cut(s) 620
FspBI CTAG 5 cut(s) 18, 32, 50, 794, 947
HaeIII GGCC 6 cut(s) 93, 467, 534, 674, 740, 1044
HapII CCGG 2 cut(s) 263, 320
HgaI GACGC 1 cut(s) 129
Hin1II CATG 6 cut(s) 281, 643, 889, 1106, 1151, 1165
HincII GTYRAC 2 cut(s) 778, 870
HindII GTYRAC 2 cut(s) 778, 870
HindIII AAGCTT 1 cut(s) 1097
HinfI GANTC 4 cut(s) 222, 297, 634, 1049
HpaII CCGG 2 cut(s) 263, 320
HphI GGTGA 3 cut(s) 196, 292, 695
Hpy166II GTNNAC 4 cut(s) 778, 870, 892, 1189
Hpy188I TCNGA 4 cut(s) 427, 432, 574, 1060
Hpy188III TCNNGA 4 cut(s) 320, 850, 1067, 1103
Hpy8I GTNNAC 4 cut(s) 778, 870, 892, 1189
Hpy99I CGWCG 2 cut(s) 131, 405
HpyAV CCTTC 3 cut(s) 224, 511, 1028
HpyCH4III ACNGT 5 cut(s) 127, 336, 838, 907, 1123
HpyCH4IV ACGT 3 cut(s) 100, 528, 1071
HpyCH4V TGCA 9 cut(s) 8, 217, 557, 650, 668, 892, 964, 1145, 1151
HpyF10VI GCNNNNNNNGC 9 cut(s) 14, 50, 90, 110, 113, 116, 168, 671, 1041
HpyF3I CTNAG 4 cut(s) 209, 252, 864, 1031
HpySE526I ACGT 3 cut(s) 100, 528, 1071
Hsp92II CATG 6 cut(s) 281, 643, 889, 1106, 1151, 1165
Kpn2I TCCGGA 1 cut(s) 319
Kzo9I GATC 3 cut(s) 427, 687, 841
LmnI GCTCC 4 cut(s) 175, 589, 730, 1075
Lsp1109I GCAGC 9 cut(s) 56, 91, 94, 97, 103, 617, 649, 652, 806
LweI GCATC 2 cut(s) 572, 951
MabI ACCWGGT 1 cut(s) 389
MaeI CTAG 5 cut(s) 18, 32, 50, 794, 947
MaeII ACGT 3 cut(s) 100, 528, 1071
MaeIII GTNAC 6 cut(s) 184, 200, 298, 635, 802, 1198
MalI GATC 3 cut(s) 429, 689, 843
MboI GATC 3 cut(s) 427, 687, 841
MboII GAAGA 4 cut(s) 135, 822, 838, 865
MhlI GDGCHC 2 cut(s) 620, 894
MlsI TGGCCA 1 cut(s) 93
MluNI TGGCCA 1 cut(s) 93
MlyI GAGTC 3 cut(s) 306, 643, 1043
MmeI TCCRAC 1 cut(s) 1026
Mox20I TGGCCA 1 cut(s) 93
Mph1103I ATGCAT 1 cut(s) 966
MroI TCCGGA 1 cut(s) 319
MscI TGGCCA 1 cut(s) 93
MseI TTAA 4 cut(s) 269, 311, 522, 1095
MslI CAYNNNNRTG 2 cut(s) 99, 243
Msp20I TGGCCA 1 cut(s) 93
MspA1I CMGCKG 1 cut(s) 482
MspCI CTTAAG 1 cut(s) 1094
MspI CCGG 2 cut(s) 263, 320
MspR9I CCNGG 6 cut(s) 173, 304, 391, 416, 771, 880
Mva1269I GAATGC 1 cut(s) 1177
MvaI CCWGG 6 cut(s) 173, 304, 391, 416, 771, 880
MwoI GCNNNNNNNGC 9 cut(s) 14, 50, 90, 110, 113, 116, 168, 671, 1041
NdeII GATC 3 cut(s) 427, 687, 841
NheI GCTAGC 1 cut(s) 17
NlaIII CATG 6 cut(s) 281, 643, 889, 1106, 1151, 1165
NlaIV GGNNCC 3 cut(s) 388, 420, 732
NmeAIII GCCGAG 2 cut(s) 736, 1070
NmuCI GTSAC 3 cut(s) 184, 298, 635
NsiI ATGCAT 1 cut(s) 966
NspI RCATGY 1 cut(s) 1151
OliI CACNNNNGTG 1 cut(s) 99
PagI TCATGA 1 cut(s) 1102
PceI AGGCCT 1 cut(s) 674
PcsI WCGNNNNNNNCGW 1 cut(s) 708
PctI GAATGC 1 cut(s) 1177
PfeI GAWTC 1 cut(s) 222
PflMI CCANNNNNTGG 1 cut(s) 541
PleI GAGTC 3 cut(s) 305, 642, 1043
PpsI GAGTC 3 cut(s) 305, 642, 1043
Ppu21I YACGTR 1 cut(s) 529
PsiI TTATAA 1 cut(s) 993
Psp6I CCWGG 6 cut(s) 171, 302, 389, 414, 769, 878
PspGI CCWGG 6 cut(s) 171, 302, 389, 414, 769, 878
PspN4I GGNNCC 3 cut(s) 388, 420, 732
PspPI GGNCC 4 cut(s) 387, 418, 532, 739
PstNI CAGNNNCTG 1 cut(s) 806
PvuII CAGCTG 1 cut(s) 482
RsaI GTAC 3 cut(s) 547, 1130, 1190
RsaNI GTAC 3 cut(s) 546, 1129, 1189
RseI CAYNNNNRTG 2 cut(s) 99, 243
SaqAI TTAA 4 cut(s) 269, 311, 522, 1095
Sau3AI GATC 3 cut(s) 427, 687, 841
Sau96I GGNCC 4 cut(s) 387, 418, 532, 739
SchI GAGTC 3 cut(s) 306, 643, 1043
ScrFI CCNGG 6 cut(s) 173, 304, 391, 416, 771, 880
SduI GDGCHC 2 cut(s) 620, 894
SexAI ACCWGGT 1 cut(s) 389
SfaNI GCATC 2 cut(s) 572, 951
SinI GGWCC 2 cut(s) 387, 418
SmiMI CAYNNNNRTG 2 cut(s) 99, 243
SmlI CTYRAG 1 cut(s) 1094
SmoI CTYRAG 1 cut(s) 1094
SseBI AGGCCT 1 cut(s) 674
SsiI CCGC 3 cut(s) 113, 260, 1041
SspMI CTAG 5 cut(s) 18, 32, 50, 794, 947
StuI AGGCCT 1 cut(s) 674
StyD4I CCNGG 6 cut(s) 171, 302, 389, 414, 769, 878
StyI CCWWGG 2 cut(s) 12, 1176
TaaI ACNGT 5 cut(s) 127, 336, 838, 907, 1123
TaiI ACGT 3 cut(s) 103, 531, 1074
TaqI TCGA 1 cut(s) 61
TaqII GACCGA 1 cut(s) 692
TatI WGTACW 2 cut(s) 1128, 1188
TauI GCSGC 3 cut(s) 116, 262, 1044
TfiI GAWTC 1 cut(s) 222
Tru1I TTAA 4 cut(s) 269, 311, 522, 1095
Tru9I TTAA 4 cut(s) 269, 311, 522, 1095
TscAI CASTG 3 cut(s) 546, 652, 841
TseFI GTSAC 3 cut(s) 184, 298, 635
Tsp45I GTSAC 3 cut(s) 184, 298, 635
TspDTI ATGAA 5 cut(s) 471, 611, 656, 1091, 1147
TspRI CASTG 3 cut(s) 546, 652, 841
Van91I CCANNNNNTGG 1 cut(s) 541
Vha464I CTTAAG 1 cut(s) 1094
VneI GTGCAC 1 cut(s) 890
VpaK11BI GGWCC 2 cut(s) 387, 418
XapI RAATTY 3 cut(s) 313, 434, 974
XceI RCATGY 1 cut(s) 1151
XcmI CCANNNNNNNNNTGG 1 cut(s) 883
XspI CTAG 5 cut(s) 18, 32, 50, 794, 947
Zsp2I ATGCAT 1 cut(s) 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.