FvH4_7g03580

DNAJ heat shock N-terminal domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001405
Physical Location & Seq
Forward (+)
3962783 .. 3964158
1376 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g03580.t1

Sequence Viewer

Length: 594 bp
ATGGCAAGCAGTGACTTACTTCCTCAAGATCCTCTCGTCAACCTGACCAAGTTGGCTGCTGTGAAGGCACGAGATACTGCTGAAGAGCTTTTCAAGCTTCAATACTTCGAGATGGCGATCATGCAGGCCATGGTGGCCAAAGAATTCGACCCCGATTTGCAAGGAGTTGATCACTTAATCCTAGCGTACCAGATCCATAAAGCCGTGGCAGAGAAGAAAGATCGGTACCATGTTCTTGGCTTCCAACAGGTTACTGACTATGTAACCCTCAAGAAACAATACAAAGAATTGGCTCGCTTTGTGCACCCAGACAAGAACAGTTCTGTTGCTGCAGAAGGGGCTTTCAAGCATGTCAAAGCGGCATGGGACTTTCTCTCTGACCCTGCCAAAAAGAAGGCCTATGACAAGTCGTTAAGGACTCCGCAGTTTCAAGGGTCCAATGTCAAGAACACTCGCAAGAGGGCTTGCCCTAAGGGGAAGACAGCCACTACTGCTGGAGATGGTCTTTTCAAGAAGACTATCAAGATAATGAGGACAACAGCTGCAGGCCAAAATACCTGTTGTATGAGGATATCTGTTTGCAGGATTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

198

Amino Acids

22.07

Weight (kDa)

9.61

Isoelectric Point (pI)

22.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 75 - 135 2.8e-16 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 225
AccB1I GGYRCC 1 cut(s) 225
AciI CCGC 2 cut(s) 359, 422
AclWI GGATC 2 cut(s) 23, 187
AcoI YGGCCR 1 cut(s) 135
AcsI RAATTY 1 cut(s) 143
AcuI CTGAAG 1 cut(s) 102
AfaI GTAC 2 cut(s) 188, 227
AgsI TTSAA 5 cut(s) 94, 101, 346, 431, 511
AluBI AGCT 3 cut(s) 88, 97, 542
AluI AGCT 3 cut(s) 88, 97, 542
Alw21I GWGCWC 1 cut(s) 306
Alw44I GTGCAC 1 cut(s) 302
AlwI GGATC 2 cut(s) 23, 187
AoxI GGCC 4 cut(s) 126, 135, 396, 547
ApaLI GTGCAC 1 cut(s) 302
ApeKI GCWGC 3 cut(s) 56, 329, 542
ApoI RAATTY 1 cut(s) 143
Asp718I GGTACC 1 cut(s) 225
AspS9I GGNCC 1 cut(s) 435
AvaII GGWCC 1 cut(s) 435
AxyI CCTNAGG 1 cut(s) 471
BaeGI GKGCMC 1 cut(s) 306
BalI TGGCCA 1 cut(s) 137
BanI GGYRCC 1 cut(s) 225
BauI CACGAG 1 cut(s) 69
BbsI GAAGAC 2 cut(s) 485, 521
Bbv12I GWGCWC 1 cut(s) 306
BbvI GCAGC 3 cut(s) 43, 316, 529
BccI CCATC 2 cut(s) 106, 494
BceAI ACGGC 1 cut(s) 188
BclI TGATCA 1 cut(s) 169
BfaI CTAG 1 cut(s) 182
BfmI CTRYAG 2 cut(s) 330, 543
BglI GCCNNNNNGGC 1 cut(s) 134
BisI GCNGC 4 cut(s) 57, 330, 360, 543
BlsI GCNGC 4 cut(s) 58, 331, 361, 544
Bme18I GGWCC 1 cut(s) 435
BmgT120I GGNCC 1 cut(s) 435
BmiI GGNNCC 2 cut(s) 227, 436
BpiI GAAGAC 2 cut(s) 485, 521
BpmI CTGGAG 1 cut(s) 516
BpuEI CTTGAG 2 cut(s) 9, 254
BsaBI GATNNNNATC 1 cut(s) 116
BsaJI CCNNGG 2 cut(s) 129, 204
Bse21I CCTNAGG 1 cut(s) 471
Bse8I GATNNNNATC 1 cut(s) 116
BseDI CCNNGG 2 cut(s) 129, 204
BseJI GATNNNNATC 1 cut(s) 116
BseSI GKGCMC 1 cut(s) 306
BseXI GCAGC 3 cut(s) 43, 316, 529
BshFI GGCC 4 cut(s) 128, 137, 398, 549
BshNI GGYRCC 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 306
BslFI GGGAC 1 cut(s) 380
BsmFI GGGAC 1 cut(s) 380
BsnI GGCC 4 cut(s) 128, 137, 398, 549
Bsp1286I GDGCHC 1 cut(s) 306
Bsp143I GATC 5 cut(s) 28, 117, 169, 192, 220
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 2 cut(s) 359, 422
BspANI GGCC 4 cut(s) 128, 137, 398, 549
BspLI GGNNCC 2 cut(s) 227, 436
BspMAI CTGCAG 2 cut(s) 334, 547
BspPI GGATC 2 cut(s) 23, 187
BspQI GCTCTTC 1 cut(s) 78
BspT107I GGYRCC 1 cut(s) 225
BssECI CCNNGG 2 cut(s) 129, 204
BssMI GATC 5 cut(s) 28, 117, 169, 192, 220
BssSI CACGAG 1 cut(s) 69
BssT1I CCWWGG 1 cut(s) 129
Bst2BI CACGAG 1 cut(s) 69
Bst4CI ACNGT 1 cut(s) 320
Bst6I CTCTTC 1 cut(s) 78
BstC8I GCNNGC 5 cut(s) 7, 126, 295, 466, 547
BstDEI CTNAG 1 cut(s) 471
BstDSI CCRYGG 2 cut(s) 129, 204
BstKTI GATC 5 cut(s) 31, 120, 172, 195, 223
BstMBI GATC 5 cut(s) 28, 117, 169, 192, 220
BstMWI GCNNNNNNNGC 5 cut(s) 65, 94, 134, 338, 491
BstNSI RCATGY 1 cut(s) 353
BstSFI CTRYAG 2 cut(s) 330, 543
BstSLI GKGCMC 1 cut(s) 306
BstV1I GCAGC 3 cut(s) 43, 316, 529
BstV2I GAAGAC 2 cut(s) 485, 521
BstX2I RGATCY 2 cut(s) 28, 192
BstXI CCANNNNNNTGG 1 cut(s) 236
BstYI RGATCY 2 cut(s) 28, 192
Bsu36I CCTNAGG 1 cut(s) 471
BsuRI GGCC 4 cut(s) 128, 137, 398, 549
BtgI CCRYGG 2 cut(s) 129, 204
BtsI GCAGTG 1 cut(s) 16
BtsIMutI CAGTG 1 cut(s) 16
Cac8I GCNNGC 5 cut(s) 7, 126, 295, 466, 547
Cfr13I GGNCC 1 cut(s) 435
Csp6I GTAC 2 cut(s) 187, 226
CviAII CATG 5 cut(s) 121, 130, 230, 350, 363
CviQI GTAC 2 cut(s) 187, 226
DdeI CTNAG 1 cut(s) 471
DpnI GATC 5 cut(s) 30, 119, 171, 194, 222
DpnII GATC 5 cut(s) 28, 117, 169, 192, 220
EaeI YGGCCR 1 cut(s) 135
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
Eco130I CCWWGG 1 cut(s) 129
Eco147I AGGCCT 1 cut(s) 398
Eco32I GATATC 1 cut(s) 573
Eco47I GGWCC 1 cut(s) 435
Eco57I CTGAAG 1 cut(s) 102
Eco81I CCTNAGG 1 cut(s) 471
EcoRI GAATTC 1 cut(s) 143
EcoRV GATATC 1 cut(s) 573
EcoT14I CCWWGG 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 129
FaeI CATG 5 cut(s) 124, 133, 233, 353, 366
FaiI YATR 9 cut(s) 122, 131, 198, 231, 261, 351, 364, 402, 566
FaqI GGGAC 1 cut(s) 380
FatI CATG 5 cut(s) 120, 129, 229, 349, 362
FbaI TGATCA 1 cut(s) 169
Fnu4HI GCNGC 4 cut(s) 57, 330, 360, 543
Fsp4HI GCNGC 4 cut(s) 57, 330, 360, 543
FspBI CTAG 1 cut(s) 182
GluI GCNGC 4 cut(s) 57, 330, 360, 543
GsuI CTGGAG 1 cut(s) 516
HaeIII GGCC 4 cut(s) 128, 137, 398, 549
Hin1II CATG 5 cut(s) 124, 133, 233, 353, 366
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 95
HinfI GANTC 1 cut(s) 418
Hpy166II GTNNAC 2 cut(s) 40, 304
Hpy188I TCNGA 1 cut(s) 379
Hpy188III TCNNGA 6 cut(s) 26, 109, 271, 445, 511, 523
Hpy8I GTNNAC 2 cut(s) 40, 304
HpyAV CCTTC 3 cut(s) 58, 329, 388
HpyCH4III ACNGT 1 cut(s) 320
HpyCH4V TGCA 6 cut(s) 124, 160, 304, 332, 545, 582
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 94, 134, 338, 491
HpyF3I CTNAG 1 cut(s) 471
Hsp92II CATG 5 cut(s) 124, 133, 233, 353, 366
KpnI GGTACC 1 cut(s) 229
Ksp22I TGATCA 1 cut(s) 169
Kzo9I GATC 5 cut(s) 28, 117, 169, 192, 220
LguI GCTCTTC 1 cut(s) 78
Lsp1109I GCAGC 3 cut(s) 43, 316, 529
MaeI CTAG 1 cut(s) 182
MaeIII GTNAC 3 cut(s) 11, 250, 262
MalI GATC 5 cut(s) 30, 119, 171, 194, 222
MboI GATC 5 cut(s) 28, 117, 169, 192, 220
MboII GAAGA 4 cut(s) 95, 226, 490, 526
MflI RGATCY 2 cut(s) 28, 192
MhlI GDGCHC 1 cut(s) 306
MlsI TGGCCA 1 cut(s) 137
MluCI AATT 2 cut(s) 143, 287
MluNI TGGCCA 1 cut(s) 137
MlyI GAGTC 1 cut(s) 412
MmeI TCCRAC 1 cut(s) 268
MnlI CCTC 6 cut(s) 33, 42, 278, 453, 525, 561
Mox20I TGGCCA 1 cut(s) 137
MscI TGGCCA 1 cut(s) 137
MseI TTAA 2 cut(s) 176, 413
Msp20I TGGCCA 1 cut(s) 137
MspA1I CMGCKG 1 cut(s) 542
MwoI GCNNNNNNNGC 5 cut(s) 65, 94, 134, 338, 491
NcoI CCATGG 1 cut(s) 129
NdeII GATC 5 cut(s) 28, 117, 169, 192, 220
NlaIII CATG 5 cut(s) 124, 133, 233, 353, 366
NlaIV GGNNCC 2 cut(s) 227, 436
NmuCI GTSAC 1 cut(s) 11
NspI RCATGY 1 cut(s) 353
PceI AGGCCT 1 cut(s) 398
PciSI GCTCTTC 1 cut(s) 78
PkrI GCNGC 4 cut(s) 58, 331, 361, 544
PleI GAGTC 1 cut(s) 412
PpsI GAGTC 1 cut(s) 412
PspN4I GGNNCC 2 cut(s) 227, 436
PspPI GGNCC 1 cut(s) 435
PstI CTGCAG 2 cut(s) 334, 547
PsuI RGATCY 2 cut(s) 28, 192
PvuII CAGCTG 1 cut(s) 542
RsaI GTAC 2 cut(s) 188, 227
RsaNI GTAC 2 cut(s) 187, 226
SapI GCTCTTC 1 cut(s) 78
SaqAI TTAA 2 cut(s) 176, 413
SatI GCNGC 4 cut(s) 57, 330, 360, 543
Sau3AI GATC 5 cut(s) 28, 117, 169, 192, 220
Sau96I GGNCC 1 cut(s) 435
SchI GAGTC 1 cut(s) 412
SduI GDGCHC 1 cut(s) 306
SetI ASST 6 cut(s) 45, 90, 99, 252, 544, 560
SfcI CTRYAG 2 cut(s) 330, 543
SfiI GGCCNNNNNGGCC 1 cut(s) 134
SinI GGWCC 1 cut(s) 435
SmlI CTYRAG 2 cut(s) 24, 269
SmoI CTYRAG 2 cut(s) 24, 269
Sse9I AATT 2 cut(s) 143, 287
SseBI AGGCCT 1 cut(s) 398
SsiI CCGC 2 cut(s) 359, 422
SspMI CTAG 1 cut(s) 182
StuI AGGCCT 1 cut(s) 398
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 1 cut(s) 320
TaqI TCGA 2 cut(s) 108, 147
TasI AATT 2 cut(s) 143, 287
TauI GCSGC 1 cut(s) 362
Tru1I TTAA 2 cut(s) 176, 413
Tru9I TTAA 2 cut(s) 176, 413
TscAI CASTG 1 cut(s) 16
TseFI GTSAC 1 cut(s) 11
TseI GCWGC 3 cut(s) 56, 329, 542
Tsp45I GTSAC 1 cut(s) 11
TspRI CASTG 1 cut(s) 16
VneI GTGCAC 1 cut(s) 302
VpaK11BI GGWCC 1 cut(s) 435
XapI RAATTY 1 cut(s) 143
XceI RCATGY 1 cut(s) 353
XspI CTAG 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.